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General Minkowski type and related inequalities for seminormed fuzzy integrals published in

Sahand Communications in Mathematical Analysis

Published by University of Maragheh
Bayaz Daraby, Fatemeh Ghadimi Minkowski type inequalities for the seminormed fuzzy integrals on abstract spaces are studied in a rather general form. Also related inequalities to Minkowski type inequality for the seminormed fuzzy integrals on abstract spaces are studied. Several examples are given to illustrate the validity of theorems. Some results on Chebyshev and Minkowski type inequalities are obtained.
A Study on Ethical Implications in Different Business Functional Applications published in

International Journal of Research Review in Engineering and Management

Published by IJRREM
S.Prasanna, S. Abishek Abstract Business ethics is dealing with the judgment revolves around right or wrong, good or bad. Besides every business people need to conduct their business operations without any impartial treatment of one with another. This research paper focuses on different form of ethical implications under various functional areas such as marketing, human resources, production, systems etc. Any business has to consider all aspects of ethical practices in order to survive in the competitive market place. There are number of theories focuses on ethical form of functional applications to satisfy the stakeholders at large. The customer are the backbone for any business operations, hence the business need to be fair and loyal with their target audience. Corporate social responsibility (CSR) plays vital role in attaining sustainable position in the market. Besides different functional heads need to realize the value of ethical implications in their business strategies and practices. Keywords: Judgment, impartial treatment, functional areas, stakeholders.
Biocontrol of Amaranthus retroflexus and Rumes crispus by NLP phytotoxine, a selective bioherbicide published in

Journal of Applied Biotechnology Reports

Published by Baqiyatallah University of Medical Sciences
Fatemeh Shakeri, Ali Mohammad Latifi, Morteza Mirzaei, Hamid Babavalian, Fatemeh Hashemlou Non-beneficial and harmful weeds are plants that are unwanted, outside their home farms are growing and have the potential to exceed. This study was done in order to screening fungai and isolating NLP phytotoxine from them for selective biocontrol of Amaranthus retroflexus and Rumes crispus as a dicot, common and chemical herbicide resistance weeds. NLPs are effective just on dicot plants. Contaminated soil and dicotyledons plants were Collected from different regions of Iran. after collecting and culturing them, The effect of Supernatant from fungal cultures, was asseyd by spraying of 5 µl /cm3 of it mid 20 µl tween-20 on leaves of Amaranthus retroflexus, Rumes crispus and wheat as negative control that were cultured in MS media and pots in 3 replications with completely randomized design in laboratory and research green house of baqiatallah university. The effects were assessed according to numbering method. Finally, the QAT5and G7-1strains was selected from 9 top strains, because was more destructive than others on Amaranthus retroflexus and Rumes crispus respectively from necrosis to cell death with number 4 according to numbring method and has non-harmful effect on the wheat (Triticum aestivum). SDS-page results showed phytotoxine that was produced by QAT5 strain was a protein and this from G7-1 was non-protein. For better result on SDS-page protein was concentrated using by ammonium sulfat method, but about G7-1 again this outcomewas repeated. The protein purification of QAT5strain using FPLC showed the presence of a protein with about 24 kDa like other family members of this protein. Considering this fact that these phytotoxines according to the result had similarity features to what founded befor about NLPs, they are recommended as biocontrol factor of these weeds insteade of chemical herbicides.
A Multidisciplinary Approach to the Functional and Esthetic Rehabilitation of a Patient with Bulimia Nervosa: A Clinical Report published in

International Journal of Dentistry and Oral Science (IJDOS)

Published by SciDoc Publishers
Pantzari F1*, Kamposiora P2, Papavasiliou G2 Treatment of a patient with bulimia nervosa is a challenge for the dental clinician. The oral manifestations of bulimic behavior, which include sore and inflamed throat, swollen salivary glands, tooth wear and decay, require a multidisciplinary approach. This clinical report illustrates the treatment of a 22-year-old female patient with history of bulimia nervosa and dentition with extended signs of erosion. It included thorough diagnosis with the use of screening tool BITE (Bulimic Investigatory Test, Edinburgh) and initial evaluation of tooth wear with the TWI (Tooth Wear Index). Crown lengthening and prosthetic rehabilitation with all- ceramic restorations on anterior teeth and metal- ceramic single crowns on posterior teeth was the treatment of choice.
A Patient with a Rare Chromosome Deletion Presents with Evidence of Spontaneous Resolution of Primary Congenital Glaucoma - Special Issue published in

International Journal of Ophthalmology & Eye Science (IJOES)

Published by SciDoc Publishers
Walker K Background: Deletions of chromosome 6q25 are very rare. Patients with these deletions present with variable characteristics depending on the location, size and break points of the deletion. In the literature it has been associated with developmental delays, brain abnormalities, hearing impairments, as well as other variable anomalies. There have been ocular abnormalities reported including retinal pigment irregularities and strabismus. Depending on their impairments, these patients can have a normal life expectancy. To our knowledge there have been no reports of congenital glaucoma with thischromosome deletion. Case Report: A 19 month old Hispanic male was referred to our clinic by his infant development teacher, who noticed that he was not visually engaged during therapy. Hewas born with a rare chromosome deletion of 6q25. He is severely developmentally delayed in all areas. He also has absence seizures for which he is prescribed Keppra and Phenobarbital. He had a previous eye examination at 3 weeks of age. In reviewing those records, he presented with a chief complaint of watery eyes OU. He was diagnosed with having blond fundus with all findings being within normal limits. The plan was to follow up in 6 weeks. The parents elected not to return for their 6 week appointment, due to dissatisfaction with the previous visit. His examination at our clinic reveled large cornea’sOU, restricted visual field OS worse than OD, obvious preference for use of his right eye, HaabStriae OS>OD, andcup to disc ratios of 0.80/0.80 OD, 0.95/0.95 OS. IOP measured by Tonopen was 18 mmHg OD and 13 mmHg OS. The patient was referred for evaluation and treatment with a pediatric ophthalmologist. Conclusion: To the best of our knowledge this is the first case that has been reported of congenital glaucoma in the presence of this rare deletion. The initial symptoms of epiphora, which can be a sign of congenital glaucoma, were not addressed in this patient’s first eye examination. While this case was an atypical presentation of congenital glaucoma, with normal IOP, there are indications that the IOP was previously elevated, indicating possible resolution of condition.
A Review on Engineering of Organophosphorus Hydrolase (OPH) Enzyme published in

Journal of Applied Biotechnology Reports

Published by Baqiyatallah University of Medical Sciences
Gholamreza Farnoosh, Ali Mohammad Latifi Organophosphorus chemicals are compounds which have been used as pesticides and insecticides in agriculture. They’re also used as nervous agents and have raised many problems for human and environment. Among the most important methods of decontamination from these compounds are biodegradation methods. Using OPH enzyme in degradation the mentioned compounds is seen as one of the desirable ways, but low activity and specification and low thermostability are among factors significantly decreasing the optimal application of this enzyme. Using methods of protein engineering based on the alteration of specific protein positions in order to improve the activity, specification and thermostability are some common ways used currently. Numerous studies have been done to increase activity and thermostability of OPH enzyme with alteration of some special amino acids the result of which was an increase against different substrates. OPH enzyme active site connected to substrates that consisted of three large, small and releasing packets were one of the goal areas of changing amino acids used by researchers to improve engineered activities. Among other ways of making enzymes more rigid and stable were bending loops by replacing Proline, creating disulfide bonds, ionic bonds by replacing charged amino acids.
A Systematic Review of Invasive Fungal Infection Treatment With Voriconazole And Caspofungin Among Chinese Population published in

Published by
Feng S1, Long Y2, Tan SC3*, Chen C4 Background: Voriconazole has been available in China for 10 years. In this time, there has been clinical studies published on the Chinese population. Objective: This study aimed to conduct a systematic review for both English and Chinese literature on the clinical efficacy and safety of voriconazole versus caspofungin in treating Chinese patients with invasive fungal infection (IFI). Methods: Both English and Chinese terms of “voriconazole” and “infection” were searched accordingly for clinical studies conducted on populations in China, Taiwan, Hong Kong and Macau in several databases. Identified abstracts were filtered independently by two researchers to select eligible studies. A third researcher was consulted for any disagreement. Jadad scale and modified Newcastle-Ottawa tool were used to assess the study quality of the selected studies. Results: Two RCTs and 10 retrospective observational studies were included in the systematic review. Conflicting trends were reported on effective treatment rate in the RCTs. 8 observational studies demonstrated that voriconazole had similar or higher effective treatment rate than caspofungin. In terms of mortality, a lower rate was generally observed among patients treated with voriconazole than those who were treated with caspofungin (0 to 26% vs. 14.3 to 50%) in 5 of the 6 studies that reported mortality rates. Conclusion: The trend of higher effective treatment rates and lower mortality rates of voriconazole compared to caspofungin were observed among Chinese patients with IFI. However, given the small sample size, low study quality, different definitions of efficacy and presence of varied underlying comorbidities in the reviewed studies, the results need to be interpreted with caution.
Analysis of Psycho Social Stresses In Family History of Suicide Victims by Suicidal Hanging published in

Published by
Malini. S1, Roy A2* The third causes of death among youths worldwide is suicide. Suicide rate in India is approximately 11.4 per 1 lakh in male and 8.0 per 1 lakh in female. In Indian study predominant suicidal victims are males, literates, married and from rural background. Aim of the present study is to asses Psychological factors, socio demographic characteristic in relation to family history of suicide associated with complete suicide by hanging. Out of 128 suicidal cases 80 cases which meet the criteria were selected for the present study. Data were collected using semi structured performer for the demographic, psychological, social, tying pattern. Knot analysis for type and handedness were analysed for family history and without the family history of suicide cases. More than 60% of suicide victims were in the age group of 15 to 34 yrs. Male outnumbered females in this study. Psychosocial stress were found in 71% of suicide victims. Granny or Reef knots were the most preferred used for suicidal hanging with family history of suicide. Using right hand proved left hemisphere dominance. Specific focus on identifying psychosocial factors, demographic variable, and increase risk of suicide can be identified at an early stage. The present study indicates that Suicide prevention strategies through family education programme can prevent the rate of suicide with family history and without family history of suicide.
Anesthetic Management of a Patient with Polycythemia Vera for Nephrectomy published in

Published by
Sethi S*, Kumari K Polycythemia Vera (PV) is a chronic myeloproliferative disease, characterized by erythrocytosis and hyperviscosity. A high proportion of PV surgeries are complicated by thrombosis (7.7%) or by a major hemorrhage (7.3%), increasing morbidity and mortality among these patients. We report a 28-year-old male patient with PV and renal cell carcinoma posted for nephrectomy. He was found to have a hematocrit of 68% on routine work-up. Preoperatively he was phlebotomized 4 times to decrease hematocrit to 48%. Perianesthetic considerations and anesthetic management of this patient are discussed. Pretreatment of PV with phlebotomy, adequate hydration, and recognition of thrombotic and bleeding problems decreases perioperative morbidity and mortality and are the keystones in the successful anesthetic management of such patients.
Assessment of Emergency Medicine Resident Accuracy and Effect of a Training Intervention in the Determination of Cervical Size and Effacement Using a Standardized Cervical Training and Examination Model published in

Mathews Journal of Emergency Medicine

Published by Mathews International Publishers LLC
James Espinosa, Department of Emergency Medicine, Rowan University SOM Kennedy University Hospital, 18 East Laurel Road, Stratford, NJ 08084, USA Women who present to the Emergency Department in their second or third trimester complaining of abdominal pain or cramps require an expeditious exam and determination of their status of possible labor. The discernment of cervical size is a core physical finding in assessing the progress of labor. The objectives of this study were to determine the accuracy of emergency medicine residents in determination of cervical dilation and effacement using a standardized cervical training model before and after completion of a training module. The results of this study suggest that a training intervention, using a standardized cervical dilatation and effacement model and associated training module, is associated with a statistically significant improvement in post-test scores in comparison to pre-test scores. It would appear that the training module itself was the prime determinant of this change. Such an increase in accuracy would logically allow an emergency resident to more accurately convey a patient’s status, to convey this status to an obstetric physician, and more importantly, make a suitable decision on transferring a patient to definitive care in a safe and timely manner. Future research could involve testing the duration and durability of the training effect.
Cleaning Effectiveness of EndoActivator Irrigation After Single-File and Multi-File Instrumentation Systems published in

International Journal of Dentistry and Oral Science (IJDOS)

Published by SciDoc Publishers
Salman MI* Objective: To compare cleaning effectiveness of two reciprocating single-file systems with ProTaper rotary instruments after one minute EndoActivator irrigation. Methods: Thirty three extracted human maxillary and mandibular single rooted human teeth were divided into three groups. Canals were prepared to the same apical size (25/.08) using Reciproc (Group I), WaveOne (Group II) or F2 ProTaper (Group III). Then all canals received additional one minute activation by EndoActivator size 25/.04. Roots were split and examined with SEM, the presence of debris and smear layer on coronal, middle and apical thirds was evaluated. Data were analyzed with the Kruskal-Wallis and Man-Whitney tests. Results: One minute EndoActivator irrigation after ProTaper instrumentation achieved significantly better results (P < 0.05) than after instrumentation with either WaveOne or Reciproc. No significant differences were obtained comparing instrumentation with Reciproc or WaveOne (P > 0.05). Conclusion: EndoActivator irrigation after a multi-file rotary instrumentation resulted in less debris and smear layer than single-file rotary systems.
Clinical, Biochemical and Bacteriological Investigation of Pneumonia in Calves with Special Reference to Alpha-1-Acid Glycoprotein Response published in

Published by
Almujalli AM1, El-Deeb WM1,2*,Eljalii EM1, Fouda TA1, AlBlwy M3 In order to investigate clinical and biochemical parameters in calves with bovine respiratory disease (BRD), twenty-five Holstein calves with clinical picture of BRD were selected to this investigation. Ten clinically healthy calves were selected as a control group. Blood, nasal and bronchoalveolar lavage were obtained from all calves under investigation. Complete blood parameters picture were investigated. Serum total protein, albumin, Triglyceride (TAG), High Density Lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), Total cholesterol, Alanine Amino Transferase (ALT), Aspartate Amino Transferase (AST), and Alkaline Phosphatase (ALP) were examined. Alpha-1-acid glycoprotein (AGP) was estimated in all calves under investigation. Bacteriological examination showed Pasteurella spp in 18 calves and Escherichia coli in seven calves. The laboratory results revealed a significant (P ? 0.05) increase in the levels of white blood cells and neutrophilia in calves with pneumonia when compared with control groups. Moreover, there was a significant (P ? 0.05) increase in the values of TAG, VLDL-c, LDL-c, ALT, AST, ALP and AGP with significant (P ? 0.05) decrease in the levels of total protein, albumin, cholesterol, HDL-c in calves with BRD when compared with control ones. From the present study, it could be concluded that AGP and lipoprotein profile could be used as diagnostic markers for BRD in calves.
Community Psychology Linking Individuals and Communities into a Scientific Psychological Framework: An Integrative Approach published in

International Journal of Translation & Community Medicine (IJTCM)

Published by SciDoc Publishers
Ganaie, S.A1*, Shah S.A1, Nahvi N.I1, Chat A.N1 Community psychology is a scientific discipline within the broad field of psychology which deals with mental health and social welfare issues of the community taking a holistic, systems-based approach to understanding behavior and how people fit in to society, much like related fields such as sociology and social psychology. Community psychology tends to be more centered on applying psychological and social knowledge to solving problems, creating real-world solutions and taking immediate action. Community psychologists primarily work in agency settings. Whether working in community health clinics involving in counseling practices and mental health work, or working in government or large social service agencies and doing research on existing social problems or planning and implementing grass roots social service programs. Their work is primarily with the marginalized and less-advantaged areas of society and those who struggle with poverty and discrimination amongst many other social ills. The primary purpose of a these psychologists is to strive for the wellbeing of an individual and society as a whole and to prevent issues from growing and treating them if they exit. The main focus of community psychologists are empowerment, social justice and wellness and prevention programs in community. These are all very broad areas of work that educate citizens to help themselves, their families and their communities to improve both their present and future. Despite the progress that has been made in Community Psychology since 1960s there is still much more improvement to be made. Societies are becoming increasingly diverse and with the continuing economic fluctuations many groups are becoming more and more marginalized. Community psychologists are working hand-in-hand with community members to identify and rectify problems as they arise and will continue to increase our knowledge regarding the society and improve the prosperity, health, well-being and lifestyles of society. Researchers have proposed one conceptual model for community crisis intervention for its development.
Comparative Effects Of Processing On The Cyanide Content Of Manihot Esculenta , Glycine Max And Zea Mays published in

International Journal of Food Science, Nutrition and Dietetics (IJFS)

Published by SciDoc Publishers
Onyeike E.N, Nwaichi E.O*, Ibigomie C.E The effects of varying processing treatments on the cyanide content of Manihot Esculenta,Zea Mays and Glycine Max were determined using picrate kit method and the following mean concentrations in ppm were obtained: 0.10, 0.00, 0.00, 0.00, 0.01, 0.00, 0.02,0.00, and 0.00 for Cassava, Garri, Fufu, Tapioka, Soybean, Vitamilk, Raw maize, Roasted maize, and Boiled maize respectively. There were statistically significant differences (P ? 0.05) among all raw samples analysed for the observed phytotoxin (Cyanide) levels. The results obtained from the processed and unprocessed food products generally showed a marked difference (P ? 0.05) in cyanide levels between raw and processed food products and this implies that food processing has a marked effect on the cyanide content of different food types indicating success in degradation of cyanide by heat. Heat treatments therefore reduced the cyanide content (approximate-ly 100%) in tested food crops thereby making them suitable and safer for consumption outside creating variety.
Comparison of Retention Forces of Different Fabrication Methods of Co-Cr Crowns: Pre-sintered and Milled, Cast and Electroforming Secondary Crowns with Different Taper Angles - Special Issue published in

International Journal of Dentistry and Oral Science (IJDOS)

Published by SciDoc Publishers
Wagner C1, Stock V1, Merk S1, Schmidlin PR2, Roos M3, Eichberger M1, Stawarczyk B1* To investigate the retention forces of differently fabricated secondary crowns made of Co-Cr alloy and electroforming secondary crowns considering different taper angles. Cobalt-chromium primary crowns with 0°, 1° and 2° taper angles were fabricated. Secondary crowns were made either by i) amilling and sintering, ii) castingor iii) electro-forming process. Pull-off tests were performed and data were analyzed by parametric statistics (p<0.05). With regard to the different taper angles, Co-Cr milled and cast groups, no impact on retention force was observed. Within the electroforming group, primary crowns with a taper angle of 1° showed a higher retention force than crowns with angles of 0° or 2°. With respect to the secondary crowns, primary crowns with a taper angle of 1° showed no impact on the results. Within the taper angles of 0° and 2°, the electroforming group exhibited lower retention forces than cast or milled ones. In the 0° taper angle group, milled secondary crowns displayed higher values than cast ones.
Determination of Maltose in Honey published in

International Journal of Food Science, Nutrition and Dietetics (IJFS)

Published by SciDoc Publishers
Fujita I Honey is an excellent nutritious food and is widely produced and marketed. However, there have been complaints that some marketed honey is adulterated with maltose. We quantified glucose, fructose, maltose and sucrose contained in honey products in the marketplace, and showed that some had been adulterated.
Determination of T-2 Mycotoxin Fusarium strains by HPLC with fluorescence detector published in

Journal of Applied Biotechnology Reports

Published by Baqiyatallah University of Medical Sciences
Reza Kachuei, Sassan Rezaie, Mohammad Hossein Yadegari, Naser Safaie, Abdol-Amir Allameh, Mohammad-Ali Aref-poor, Abbas-Ali Imani Fooladi, Majid Riazipour T-2 toxin is the most poisonous trichothecene produced by Fusarium species especiallyF.sporotrichioides. T-2 toxin is a biological contaminant in a number of agricultural commodities that can cause severe diseases among humans and animals and even lead to death. The aim of the current study is the analysis of T-2 mycotoxin in Fusarium species by high-performance liquid chromatography (HPLC) combined with florescence detection and derivatization with 1-antroylnitrile (1-AN). Totally, 11Fusariumisolates and reference strains were studied. The isolates were tested for the T-2 toxin production after growing on rice substrate followed by using specific “Multisep 225 Trich Clean up columns” purification. In this study, T-2 toxin production was ranged from 197.05ug/kg to 8503.07ug/kg. This is the first study of T-2 toxin analysis by HPLC-F in Iran.
Determination of T-2 Mycotoxin Fusarium strains by HPLC with fluorescence detector published in

Journal of Applied Biotechnology Reports

Published by Baqiyatallah University of Medical Sciences
Reza Kachuei, Sassan Rezaie, Mohammad Hossein Yadegari, Naser Safaie, Abdol-Amir Allameh, Mohammad-Ali Aref-poor, Abbas-Ali Imani Fooladi, Majid Riazipour T-2 toxin is the most poisonous trichothecene produced by Fusarium species especiallyF.sporotrichioides. T-2 toxin is a biological contaminant in a number of agricultural commodities that can cause severe diseases among humans and animals and even lead to death. The aim of the current study is the analysis of T-2 mycotoxin in Fusarium species by high-performance liquid chromatography (HPLC) combined with florescence detection and derivatization with 1-antroylnitrile (1-AN). Totally, 11Fusariumisolates and reference strains were studied. The isolates were tested for the T-2 toxin production after growing on rice substrate followed by using specific “Multisep 225 Trich Clean up columns” purification. In this study, T-2 toxin production was ranged from 197.05ug/kg to 8503.07ug/kg. This is the first study of T-2 toxin analysis by HPLC-F in Iran.
Development of an In-House Enzyme-Linked Immunosorbent Assay Based on Helicobacter Pylori Sonicate Whole Cell Antigen for Diagnosis of Gastroduodenal Ulcer Disease in Karachi, Pakistan published in

Published by
Faisal Aziz1*, Yasmeen Taj 2, Shahana Urooj. Kazmi 1 Helicobacter pylori (H. pylori) is a causative agent of gastritis, gastroduodenal ulcers and gastric adenocarcinoma. Improper diagnostic facilities are responsible for the increasing incidence of H. pylori infections in Pakistan. ELISA is a noninvasive, less expensive assay for diagnosis of H. pylori . ELISA performance is mainly based on the H. pylori strain and nature of antigen. In this study, a total of 214 gastritis and ulcer patient’s serum samples were screened for anti- H. pylori IgG antibody. A 96-well plate coated with 10 ?g/ml sonicate antigen and hundred-fold diluted patient’s serum was allowed to react. After extensive washing with buffer, 1:2,000 diluted conjugated secondary antibody was added. Later substrate was added to observe positivity by measuring the intensity of color. Statistical analyses were performed, and p value of < 0.01 was taken as significant; 84 % male patients and 96 % female patients, respectively, tested positive for H. pylori , while agewise distribution was 35–45 years males (40 %) and 35–45 years females (73 %) were found highest number of H. pylori infected patients. In-house ELISA based on sonicate whole cell antigen (sELISA) showed a sensitivity of 98 %, specificity of 100 %, accuracy 98 % and ? value 0.906 with significant correlation R—0.956; p < 0.0001. We conclude that ELISA for H. pylori sero-diagnostic infection should be based on the local strain for better sensitivity and specificity. sELISA is better and reliable diagnostic assay for the diagnosis of H. pylori infection in gastric patients of Karachi, Pakistan.
Difficult Airway Management In Patients With Severe Post Burn Contracture of Neck published in

Published by
Sarvesh1*, Shalini Chaudhary2 Severe anterior post-burn contracture of the neck poses the anaesthesiologist some difficult problems and results in difficult intubation when extension of the atlanto-occipital joint is impaired. Such patients must therefore have the post-burn contracture scar released before other procedures are performed, in order to ensure airway control. The fiber optic bronchoscope is considered safe and reliable in the difficult endotracheal intubation. Fiber optic bronchoscopy is felt to be the technique of choice when intubation is difficult especially when there are contractures or deformities of the larynx, manidible, or cervical spine.
Does Mood Affect Self-Concept? Analysis through a Natural Semantic Networks Based Approach published in

Published by
PE Flores Kanter*, LA Medrano, H Conn Currently, one of the most developed areas of research concerns the elucidation of the relationship between affect and cognition. The main objective of this experimental study is to examine the effects of mood on self-concept, or more precisely, on the elaboration or conceptualization of the self-concept in-the-moment. A useful technique that has recently started to be used in research concerning self-concept was used: The Natural Semantic Networks Technique. The data was analyzed using a graphics tracing technique, which allows an accurate visualization of the network elements (i.e. self-concept defining words) and of the centrality that the elements have in that network. The results support the hypothesis that mood affects in-the-moment judgments of self-concept.
Drug Design And Analysis InSilico of Sapelenin G, an Acyclic Triterpenoid as Potential Anti-Inflammatory published in

International Journal of Clinical Pharmacology & Toxicology (IJCPT)

Published by SciDoc Publishers
Ngabireng Marie. Claude1*, Menye Cyrille2, Kouam F.Simeon3, Ntede N .Hyppolite4, Tagoudjeu Jacques1, Awono Onana1 Diverse non-steriodal anti-inflammatory drugs and COX-2 inhibitors are a class of drugs which selectively inhibit COX-2, provide relief from pain and inflammation. However, they lack anti-thrombotic activity and hence lead to cardiovascular and renal liabilities apart from gastrointestinal irritation. To ameliorate this situation, research can be foccuss on the products originating from natural products that could offer better relief from inflammation than the currently used commercial drugs. Aspirin blocks the cyclooxygenase enzyme (COX 1, 2) which is involved in the ring closure and the addition of O2 to arachidonic acid, converting it to prostaglandins (which induce inflammation, pain and fever). The present study is undertaken to analyse the docking efficacy of aspirin with the target molecule (2AW1), to assess the best ligand for inhibiting COX and to analyze the docking program by Arguslab. Substituting aspirin ligand by sapelenin G, the finding suggests that sapelenin G is a better ligand than aspirin. It satisfies Lipinski Rule of 5. The PASS (Prediction of Activity Spectra for Substances) prediction results show that Sapelenin G has an anti-inflammatory activity. Toxicity estimations of Sapelenin G using Toxtree on humans and based on the Cramer rules, Verhaar, Structural Alerts for Reactivity in Toxtree (START) biodegradability, eye irritation/corrosion and skin irritation/corrosion fell into class 3, 5, 1, 2 and 1, respectively. Application of the Benigni-Bossa method showed that this compound is negative for genotoxic carcinogenicity and negative for nongenotoxic carcinogenicity. The cytochrome P-450 mediated drug metabolism is negative for Sapelenin G only in SMARTCyp.Rank2.sites of metabolism, and it fell into unreactive group of compounds by Michael addition. A skin sensitization evaluation reveals that the compound has no skin sensitization alert identified, moreover, Kroes Threshold of Toxicological Concern (TTC) decision tree reveals that the Substance would not be expected to be a safety concern.
ECODESIGN CRITERIA FOR COMPOSITE MATERIALS AND PRODUCTS published in

Journal of Fundamental and Applied Sciences

Published by University of El Oued
B. Attaf According to sustainable development principles, the design of a composite product must be in compliance with the new regulations and standards in terms of Health protection (H) and Environmental preservation (E) besides Quality assurance (Q). With this argument as an objective, our contribution aims to innovate and develop new methodologies providing sustainable design of new generation of composite products through the consideration of the three balanced key aspects: H, E and Q. To achieve these requirements, we have defined and developed new criteria in the form of eco-coefficients, which can later on be implemented into mechanical characterization tests, in advanced composite formulations and associated constitutive equations. They can also be integrated into future finite-element computer programs to assess and improve the performance of the H-E-Q interrelated function.
Effect of post-polymerization heat treatment on a denture base acrylic resin: histopathological analysis in rats - Special Issue published in

International Journal of Dentistry and Oral Science (IJDOS)

Published by SciDoc Publishers
Meister LM1, Kovalik AC1, Pellissari CV2, Bail M1, Campagnoli EB1, Jorge JH2*, Campanha NH1 Aims: This work examined the histological effects, on the rat palatal mucosa, of a denture base acrylic resin, submitted or not to a post-polymerization heat-treatment. Methods: Fifteen adult female Wistar rats, with sixty days old, weighting 150 g – 250 g were divided in G1: animals being maintained under the same conditions as the experimental groups following described, but without the use acrylic palatal plates (control group); G2: use of heat-polymerized acrylic resin palatal plates made of Lucitone 550; G3: use of palatal plates identical to G2, but subjected to a post-polymerization treatment in a water bath at 55°C for 60 min. The plates covered all the palate and were fixed in the molar region with light-cured resin, thus being kept there for 14 days. After the sacrifice, the palate was removed, fixed in formaldehyde 10% and decalcified with EDTA. Sections were stained using haematoxylin and eosin. Images in duplicate were made from the central region of the cuts, to measure the thickness (?m) of the keratin layers (TKC), epithelium total (TET) and connective tissue (TCC). Statistical analyses were carried out by one-way ANOVA and Tukey post-tests (?=0.05). Results: According to the results there was significant difference in the thickness of keratin between G2 and G3, with G1 having the intermediate value and similar to the other groups. There was a significant difference in the connective tissue with G3
Effects of missense R84Q mutation on human Pyrroline-5-carboxylate synthase enzyme properties, an in-silico analysis published in

Journal of Applied Biotechnology Reports

Published by Baqiyatallah University of Medical Sciences
Maryam Zare, Faranak Hadi, Zarrin Minuchehr, Jafar Amani, Ali Hatef Salmanian Mammalian ?-(1)-Pyrroline-5-carboxylate synthase (P5CS) enzyme catalyzes the coupled phosphorylation and reduction-conversion of glutamate to ?-(1)-pyrroline-5-carboxylate (P5C), a critical step in the proline, ornithine, citrulline and arginine biosynthesis. In plants and mammals, P5CS consists of two separate enzymatic domains: N-terminal ?-glutamyl kinase (?-GK) and C-terminal ?-glutamyl phosphate reductase (?–GPR). Hyperammonemia has been reported as a new inborn disorder, with a range of clinical symptoms which is associated with a reduced synthesis of proline, ornithine, citruline and arginine. A missense mutation, R84Q, which alters the conserved residue in ?-GK domain, is responsible for this disorder. In this study using in-silico approaches as a new bioinformatics method, sequence analysis was performed and the tertiary structure of ?-GK domain of human P5CS, which includes the R84Q missense mutation, was predicted and the mutation effects on structural and functional features of P5CS enzyme were analyzed. Our analysis showed that this substitution has an affect on the molecular surface accessibility and total energy of the modeled structure. We conclude that this mutation results in a reduced activity of P5CS enzyme and an impaired synthesis of these amino acids.
Effects of Ranitidine on Insulin and Lime - Induced Gastric Secretion in Albinowistar Rats published in

International Journal of Clinical Pharmacology & Toxicology (IJCPT)

Published by SciDoc Publishers
Nwaichi, E. O.1, Gwotmut, M. D.2 and Ossai, J.3 Purpose: To study the possible effect (s) of a relative H2-receptor blocker, ranitidine on lime and insulin-induced gastric secretion in male and female albino rats. Methods: The rats were divided into 3 groups of lime juice, insulin and control in triplicates after 24hr starvation to empty the stomachand were canulated (oesophageal, tracheal and gastric) using Gosh and Schild method. Using N saline, the acid content of the effluentwas recorded. The 1st group of rats was perfused with lime solution (25% v/v, 50%v/v, 75% v/v and 95% v/v) and was used to modify the secreto-ry rates of the parietal cells and the stomach effluent was collected 3 times in 30 minutes. In same man -ner, the 2nd group was perfused with 40 IU/ kg insulin. The 3rd group (control) had no lime nor insulin. Ranitidine was administered (2.5ml ) intramuscularly and the results noted. Results: The mean basal secretion significantly (P? 0.05) increased from 22.82 ±4.6mMol/L/hr to 52.94 ±10.23mMol/L/hr, while 2.5ml ranitidine (Zantac)injected intramuscularly decreased the basal stimula-tion from 52.94 ±10.23mMol/L/hr to34.77 ± 5.09 mMol/L/hr. Insulin (40 IU/kg) was administered in-travenously to the 2nd group of rats, and the mean basal secretion increased from 8.01±0.75 mMol/L/hr to10.00±0.71 mMol/L/hr. Ranitidine was administered intramuscularly and that caused a significant decrease in the insulin stimulation from 10.00±0.71 mMol/L/hr to 8.01±0.75 mMol/L/hr. Conclusion: Results obtained showed marked higher gastric secretion in females than in males, although stead increase was observed for both insulin and lime inducement.Generally, results obtained from this study indicated, a statistically significant decrease in both lime juice and insulin-induced gastric secretion by Ranitidine, and hencesubmits Ranitidine as a possible potent drug for peptic ulcer treatment.
Enhancing iron oxidation efficiency by a native strain of Acidithiobacillus ferrooxidans via response surface methodology, and characterization of proteins involved in metal resistance by proteomic approach published in

Journal of Applied Biotechnology Reports

Published by Baqiyatallah University of Medical Sciences
Fahimeh Nemati, Daryoush Arabian, Rasool Khalilzadeh, Fazin Abbaspour Aghdam The effects of different factors on growth and bio-oxidation efficiency of a native strain of Acidithiobacillus ferrooxidans have been evaluated by the utilization of response surface methodology, RSM. Medium pH and iron concentration were found to be the most significant factors while temperature and ammonia concentrations had the least weight within the ranges investigated. Optimum operational conditions for maximizing Fe2+ oxidation were found to be 31 oC, 7 g/Liron concentration, 4.5 g/Ltotal ammonium salt concentration and medium pH 1.85. Maximum recovery of 98% of Zinc is the main outcome of results as observed at 7 g/Lof Fe2+, under optimized experimental conditions. The response of a bacterial strain to metals toxicity also studied. The isolate showed good resistance to most of the toxic metals. The proteomics approach was used to identify the differentially expressed proteins under heavy metal stress. Four of the differentially expressed proteins were identified as major outer membrane protein of A. ferrooxidans, ribulose large bisphosphate carboxylase subunit, and holo synthase.
Erupted Complex Odontoma Mimicking a Mandibular Second Molar published in

International Journal of Dentistry and Oral Science (IJDOS)

Published by SciDoc Publishers
Almeida LE1*, Andrade MO2, de Oliveira Filho MA2, Trevilatto PC3, Doetzer AD3 Complex odontoma (CO) is considered one of the most common odontogenic lesions, composed by a miscellaneous of dental tissue such as enamel, dentin, pulp and sometimes cementum. They may interfere with the eruption of an associated tooth, being more prevalent in the posterior mandible. CO has been rarely reported as erupted, being considered an intraosseous lesion. This is a case report of a 17-year-old male with a benign fibro-osseous lesion consistent with CO that was located at the left second molar region, above the crown of the impacted mandibular second molar tooth. The lesion was surgically removed, and the tooth had to be extracted, since there was no indication that it could erupt naturally or with orthodontic traction. The histopathological examination confirmed the diagnosis of CO and after 6 months complete bone formation was observed radiographically. An early diagnosis will provide a better treatment option, avoiding tooth extraction or a more damaging surgery.
Facial Palsy after Carotid Endarterectomy & Difficult Intubation during Subsequent CABG Surgery: An Impact of the Cranial Nerve Injury on Airway Management published in

Published by
Won K. Chee Cranial nerve injuries can occur during carotid endarterectomy from extensive dissection and prolonged retraction.The nerve injuries canimpactboth the airway reflexand the anatomical contour to cause a difficulty with subsequent airway management for the anesthesiologists. A careful examination of the airway after the carotid procedure is critical for recognizing the subtle signs of cranial nerve injuries.
GC-MS Analysis of Chemical Components of Taxus Chinensis Var . Mairei Seeds published in

Published by
GAO HanDong 1*,ZHANG YanJie2,LU ShunBao2 The extracts of petroleum ether, methanol, ethyl acetate and ether from Taxus chinensis var. mairei seeds were analyzed by GC-MS, and relative contents were determined using a normalized method. 56 peaks were obtained from extracts of spermoderm and 79 peaks from endosperm of T. chinensis var. mairei. Among these peaks, 37 chemical constituents were gained, and 24 compounds of which were identified (64.86%). There were 35 chemical constituents with content more than 1%, accounting for 94.59% of the total of all chemical constituents. 32 peaks were gained from petroleum ether extract, and 7 peaks were identified (21.88%) with content more than 1%. 36 peaks were gained from methanol extracts, and 14 peaks were identified (38.89%), 13 of which with content more than 1%, accounting for 92.86% of the total of all chemical constituents. 34 peaks were gained from ethyl acetate extracts, while 10 peaks were identified (29.41%) with content more than 1%. 33 peaks were gained from aether extracts, and 11 peaks were identified (33.33%). T. chinensis var. mairei seeds have various bio-active ingredients and a higher value for development and utilization as a medicinal plant.
Investigating the Effect of Dimercaprol on Phytoremediation of Lead by Zea mays, published in

Journal of Applied Biotechnology Reports

Published by Baqiyatallah University of Medical Sciences
Mohammad Amin Sharif, Ali Mohammad Latifi, Mohammad Ali Amani, Fatemeh Shakeri, Morteza Mirzaei, Parvin Noroozimoghadam Application of plants to remove heavy metals from soil (phytoremediation) is expanding due to its cost-effectiveness as compared to conventional methods and it has shown a great potential. Since contaminants such as Pb have a limited bioavailability in the soil, methods to facilitate their translocation to the shoots and roots of plants are required for efficient phytoremediation. The present pots experiment were to investigate the effect of Dimercaprolchelator with different concentration (0, 1.5, 3 mmol Dimercaprolchelator kg-1). Also different ranges of Pb (100, 200 mg Pb kg-1) and a control group were investigated for the amount of Pb accumulation, by corn (Zea mays), sunflower (Helianthus annuus) and mustard (Sinapis arvensis). The results showed that the amount of Pb accumulation increased as the Pb concentration was increased. Also the results of this experiment showed that the addition of Dimercaprolchelatoris most likely to increase the bioavailability of Pb and consequently the accumulation of this heavy metal in the shoots. The highest accumulation of Pb was noticed with the highest does of Dimercaprolchelator (3 mmol Dimercaprolchelator kg-1) and Pb (200 mg Pb kg-1) in the shoots of Helianthus annuus.
Investigation of the Effect of Substrate Conditions on Electron Transfer of Glucose Oxidase published in

Journal of Applied Biotechnology Reports

Published by Baqiyatallah University of Medical Sciences
Khadijeh Eskandari, Sharareh Sajjadi, Amir-Homayon Keihan, Mehdi Kamali, Jamal Rashidiani, Zahra safiri Direct electron transferring of glucose oxidase was investigated on reduced graphene and graphene oxide templates. The direct electrochemistry glucose oxidase on graphene showed a cyclic voltammograms corresponding to the FAD/FADH2 redox couple with an anodic, cathodic and formal potential of -430, -460 and -445 mV, respectively in 0.1 M phosphate buffer solution and air saturated condition for similarity of in vivo usage. The cyclic voltammograms of glucose oxidase on graphene is reversible. Also, the voltammograms results show, the current intensity of glucose oxidase on graphene is high, due to fast electron transferring. Moreover, the linear rang concentration of glucose on graphene are 0.4–9 µM. These studies make useful insight into the enzyme immobilization on nanoparticles for biosensors and bio-fuel cell preparation.
Light Emitting Diode in Comparison to Halogen Curing Technology: Microshear Bond Strength of Dental Composite Resin Restorative Material published in

International Journal of Dentistry and Oral Science (IJDOS)

Published by SciDoc Publishers
Hammouda IM1,2*, Beyari MM3 The aim of this study was to compare the micro-shear bond strength of composite resin cured with halogen visible light and blue light emitting diode curing units. Flat enamel surfaces of 50 segments of teeth were prepared. 50 cylindrical composite resin specimens were bonded to the prepared teeth segments. Specimens were divided into 5 groups, three groups were cured for 10, 20 and 40 seconds using blue light emitting diode and 2 groups were cured for 20 and 40 seconds using halogen light. Specimens were submitted to micro-shear bond test at 1mm / min cross-head speed. Mean micro-shear bond strengths were analyzed by analysis of variance (One-way ANOVA) and Tukey's test at P<0.05. At 40 seconds curing, the halogen light produced significantly higher bond strength than that of blue light emitting diode. No significant difference was found between both lights at 20 seconds. There were no significant differences in bond strength ratios between 10 and 20 seconds blue light emitting diode, and 20 seconds halogen light. At 40 seconds, the blue light emitting diode produced significantly higher bond strength than that of blue light emitting diode at10 and 20 seconds, and halogen light at 20 seconds. It was concluded that despite the higher micro-shear bond strength of halogen light at 40 seconds, blue light emitting diode curing unit provided sufficient output to exceed minimum requirements in terms of composites’ micro-shear bond strength according to ISO 11405, 1994. During incremental application of composite resin, 10 seconds blue light emitting diode curing time is sufficient to produce acceptable micro-shear bond strength values.
Low Vision Rehabilitation in the Glaucomas - Special Issue published in

International Journal of Ophthalmology & Eye Science (IJOES)

Published by SciDoc Publishers
McAllister B*, Rebecca Kammer -
LQR Controller for Kufasat published in

Journal of Kufa physics

Published by Physics Department /Faculty of Science /University of Kufa
Mohammed Chessab Mahdi, Mohammed Jaafar AL-Bermani Abstract In this paper, Linear Quadratic Regulator (LQR)controller is applied to the attitude stabilization control of Kufasat. Using the linearized equations of motion for a rigid body in space, the linearized stability, effectiveness and robustness of a linear quadratic regulator (LQR) control design were compared with that of a Proportional-Integral-Derivative (PID) control design. The detailed design procedure of the LQR controller is presented. Simulation results show that precise attitude control is accomplished and the time of satellite maneuver is shortened in spite of the uncertainty in the system.
Maintaining Metabolically Healthy Overweight and Risk of Incident Type 2 Diabetes or Cardiovascular Disease - Special Issue published in

Published by
Hashimoto Y1, Tanaka M1, Senmaru T1, Asano M1, Yamazaki M1, Oda Y1, Toda H2, Nakamura N1, Fukui M1* Background: Recent studies reveal that metabolically healthy obesity, which is free from the metabolic complications of obesity at present, is a risk of incident diabetes or cardiovascular disease (CVD). It may be because many metabolically healthy obesity individuals transit to metabolically abnormal obesity over time. However, the association between maintaining metabolically healthy obesity and incident diabetes or CVD is still unclear. Methods: 2190 participants without diabetes and/or CVD were examined at baseline and at 5-year follow-up, using a retrospective cohort study design. Metabolically abnormal was defined as having ? 2 of metabolic abnormalities, hypertension, hypertriglyceridemia, low HDL cholesterol and impaired fasting glucose. Overweight was defined as BMI ? 23 kg/ m2, which is recommended by WHO for Asian. Adjusted odds ratio (OR) and 95% confidence interval (CI) for incident diabetes or CVD at follow-up were estimated adjusting for potential confounders, compared to maintaining metabolically healthy non-overweight (MHNO). Results: At the follow-up examination, 82 participants developed diabetes. Crude incident proportions of diabetes were 0.6% in maintained MHNO (case/N = 5/859) and 1.3% in maintained metabolically healthy overweight (MHO)(4/314). Adjusted OR for incident diabetes of maintaining MHO was 1.71 (95% CI 0.42-6.57, P = 0.438). At the follow-up examination, 51 participants developed CVD. Crude incident proportions of CVD were 0.8% in maintained MHNO (7/859) and 1.6% in maintained MHO (5/314). Adjusted OR for incident CVD of maintaining MHO was 1.42 (95% CI 0.41-4.56, P = 0.565). Conclusions: Maintaining MHO phenotype was not associated with higher risk of incident diabetes or CVD.
MANAGEMENT OF A NTI - TUBERCULAR THERAPY IN DUCED HEPATITIS AND TUBERCULOUS MENINGITIS COMPLIC ATIONS IN A YOUNG ADULT PATIENT: A CASE REPORT published in

INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES

Published by Dr. CHANDRASEKHARA RAO BARU
Kandimalla. Chaitanya * , Bandlamudi. Yasaswini, Sudhakar R, Minu K George TTuberculous Meningitis (TBM) is the most dr eaded form of tuberculosis (TB) with Central Nervous system involvement which has very high morbidity and mortality rate. It is noted in 5 to 10% of extra pulmonary TB cases, and accounts for approximately 1% of all Tuberculosis cases. Multi - drug regimen i ncludes Isoniazid, Rifampin, Ethambutol, Pyrazinamide and Streptomycin along with adjunctive treatment with corticosteroids is required in the effective management of Tuberculosis meningitis. Anti - Tuberculosis Therapy induced Hepatotoxicity is the well - kno wn side effect of several drugs which are used for the treatment of TB and it is found to be 2% to 28%. We report a case of ATT induced hepatitis (Isoniazid - Rifampin) along with tuberculosis meningitis complications such as grade II Obstructive Hydrocephal us and hyponatremia and it is successful management with Inj.Mannitol and Inj.Dexamethasone. Key Words: Tuberculous Meningitis, ATT induced Hepatitis, Hydrocephalus, Hyponatremia
Metabolic Syndrome in Basic Research: How to Study It published in

International Journal of Food Science, Nutrition and Dietetics (IJFS)

Published by SciDoc Publishers
F.T. Borges1,2 , N. Schor1* -
Metabolism Of L929 Cells After Contact With Acrylic Resins. Part 2: Soft Relines - Special Issue published in

International Journal of Dentistry and Oral Science (IJDOS)

Published by SciDoc Publishers
Jorge JH*, Silva CRC, Pavarina AC, Amaya MI, Masetti P, Pellissari CV Objective: The aim of this study was evaluating the cytotoxicity of resilient relining materials used in Brazil, according to the time of water storage and heat treatment. Material and Methods: The specimens were made measuring 14 mm in diameter and 1.2 mm thick. Twelve samples of each material were prepared and divided into four groups (n = 3): Group 1: assessment of cytotoxicity immediately after the samples making; Group 2: assessment of cytotoxicity after storage of the samples in distilled water at 37° C for 24 hours; Group 3: assessment of cytotoxicity after storage of the samples in distilled water at 37° C for 48 hours; Group 4: cytotoxicity after soaking the samples in water at 55° C for 10 minutes. To prepare the extracts, 3 samples of each group were placed into vials containing 3 mL of culture medium and stored at 37° C for 24 hours. L929 cells were used and the MTT test was performed. The results were subjected to two-factor factorial analysis of variance (ANOVA) at the level of 5% significance. In addition, the materials were classified according to the cytotoxic effect: non-cytotoxic, slightly cytotoxic, moderately cytotoxic, and strongly cytotoxic. Results: The Dentuflex reliner was considered slightly cytotoxic. The other resins, compared to the control group, were classified as non-cytotoxic. Storage in water for 24 or 48 hours did not affect the cytotoxicity of lining materials tested. Conclusion: The heat-treatment reduced the number of viable cells, and Soft Comfort and Dentuflex resins were classified as slightly and moderately cytotoxic, respectively.
Microarray Data Analysis for Detection and Classification of Viral Infection published in

Journal of Applied Biotechnology Reports

Published by Baqiyatallah University of Medical Sciences
Khadijeh Nazari, Ali Karami, Nezameddin Mahdavi Amiri, Fatemeh Pourali DNA microarrays consist of collection of DNA microscopic spots that In order to form an array attached to a solid surface such as glass, plastic or silicon chip. The pieces of fixed DNA considered as a searcher. In this technology it is possible to test sample against thousands probes for specific genes. With this ability, arrays accelerate the biological investigations, gene finding, molecular detection and disease diagnosis. Microarray technology can be seen as a continued development of southern blotting. The most important stage in this technology is data analysis. To analysis such large data whit high degree of confidence and reliability needs reliable bioinformatics tools. Infectious diseases still is major problem for human. One of the most important application of microarray technology is the possibility of testing for the presence of thousands micro-organism in environmental and clinical samples only in a single excrement. Thereby we take an important step in rapid and accurate detection of infectious diseases. Here, we present E-Predict algorithm and DetectiV package that is based on species identification in microarray. We demonstrate the application of E-Predict and DetectiV for viral detection in a large publicly available dataset and show that DetectiV performs better than E-Predict. DetectiV is implemented as a package for R - powerful, open source software for statistical programming - that containing visualization, normalization and significance testing functions.
Microbiological Considerations for Probiotic Supplemented Foods published in

Published by
S. Sarkar* Functional properties of probiotics coupled with consumer’s inclination towards healthful foods have projected probiotics as a new ingredient in functional food market. Probiotic containing foods exhibits diverse health benefits and the starter cultures employed for formulation of probiotic supplemented food must possess certain prerequisite characteristics to exhibit prophylactic properties. Probiotic containing foods available in the market are often of poor quality and did not meet the desired level of viable microorganisms, required for exhibiting health benefits. In the present article an endeavor has been made to highlight the significance of probiotic viability and their population for exhibiting health benefits and the quality of probiotic containing foods available in the global market and prerequisites for identity of a product as a probiotic food have also been delineated. Production of probiotic supplemented food with prophylactic is emerging to build-up consumer’s confidence for long-term sustainability of probiotic food industries.
Microcytic Anemia Still a Health Problem in the Third Millennium published in

International Journal of Translation & Community Medicine (IJTCM)

Published by SciDoc Publishers
Eloisa Urrechaga1*, Silvia Izquierdo2, Jesus F Escanero3 -
Molecular Detection of blaTEM and blaSHF in Diarrhoegenic Escherichia coli Isolated from Egyptian Children published in

Published by
Maysaa El SZ1*, Elewa A1, Mansour AK2 Introduction: Escherichia coli (E.coli) is a common bacterial pathogen for acute diarrhea in children. The aim of the present study was to determine the prevalence mainly of TEM and SHV-beta lactamase-encoding genes responsible for extended spectrum beta lactamase (ESBL) production amongst diarrheagenic E. coli species as these strains are reported to have been increased in recent years. Methodology: The study included children with acute diarrhea presented in Mansoura University children hospital during the period from January 2011 till Jun 2014. In the microbiological laboratory stool samples were cultured and the isolated colonies were identified by standard biochemical reactions and by multiplex polymerase chain reaction (PCR) for identification of diarrheagenic E.coli strains. In vitro susceptibility testing of all isolates was performed using the discs diffusion method. E.coli isolates were subjected to testing to detect the possible presence of SHV, and TEM genes by conventional PCR. Results and Conclusion: The study included 600 children presented as acute diarrhea. Acute diarrhea caused by E.coli was detected in 160 samples. ESBL genes either TEM or SHF was detected in 31.1% of isolated E.coli. The commonest gene was TEM (22.5%), then SHF (10%). Combined genes were detected only in 2 isolates, table 6. The commonest resistance pattern of E.coli harboring ESBL genes cefepime (100%), cefazolin (96%) and cefotaxime (96%) and for non beta lactams the commonest was for ciprofloxacin (88%), amikacin and tobramycin (20% for each), Table7. From this study we can conclude that extended beta lactamase production is common among diarrheagenic Escherichia coli isolated from children below 5 years. The bla-TEM is the common genetic mechanism for extended beta lactamase production in these isolates followed by bla-SHF.
Moving The Field of Health Behaviour Research Forward with Genuine Application and Cross-Culture Assessment of Established Theoretical Models and Techniques published in

International Journal of Translation & Community Medicine (IJTCM)

Published by SciDoc Publishers
Bai Li -
Mujeres: Limitaciones supuestas vs capacidades reales published in

Religación. Revista de Ciencias Sociales y Humanidades

Published by RELIGACIÓN-RGN. Centro de Investigaciones en Ciencias Sociales y Humanidades desde América Latina
Hernan Eduardo Díaz Rubiano Las acciones de solidaridad, cuidado y reconocimiento del otro, que generalmente son asumidas por las mujeres por la demarcación de roles dentro de la familia, son vistas muchas veces como debilidades más que como capacidades. Esta situación limita las posibilidades de realización plena tanto en lo familiar como en lo laboral y las pone en desventaja frente a los hombres, exclusivamente por el solo hecho de ser mujeres. Los conceptos de virtud, cuidado y capacidad se tejen en este texto con las vivencias de una maestra que busca realizarse profesionalmente al mismo tiempo que disfruta con plenitud de su rol de madre y esposa.
Nature as an Ideal Rhythm Model for Optimal Cardiovascular Physiology and Health published in

Published by
Akbar Nikkhah -
Oral Fluid and Driving Under the Inluence of Drugs (Duid): A Brief Review published in

Published by
A Arroyo1*, MT Marrón1, MJ Leal2, C Vidal1 This review deals with different aspects of driving under the influence of drugs (DUID).Cannabis, cocaine, opiates, amphetamines and methamphetamines are the most prevalent illegal drugs detected in the surveys carried out by the police and involved in traffic accidents. The use of oral fluid matrix to detect drugged drivers as well as pharmacokinetics of illegal drugs in oral fluid, correlation with blood, times of elimination and on-site drugs tests has been considered. A brief description of the marketed on-site test devices is provided. The relationship between drug use and impairment driving, and articles published in the specialized literature have been consulted. Data has been obtained from MEDLINE base date during the last years. Although oral fluid is a suitable fluid for analysis and the a steady progress has been done along the time, in the devices for screening, more effort must be achieved regarding to its performance and reliability regarding to parameters as sensibility specificity and recovery, specially for cannabis, the most prevalent drug detected. Standardized protocols referring to sample collection, established cut-offs, analytical confirmatory methods are issues that may be established to avoid erroneous results and interpretation. At present, legislation around the world using the oral fluid in legal procedure is not well defined but some countries are applying these methods to sanction drivers. In Spain, in Catalonia region, qualitative results are being utilized to fine drivers and courts are admitting the proofs.
Production and Purification of Polyclonal Antibodies against Diphtheria Toxin published in

Journal of Applied Biotechnology Reports

Published by Baqiyatallah University of Medical Sciences
Mohammad Ali Arefpour Torabi, Gholam Reza Olad, Shahram Nazarian, Jafar Salimian, Samaneh Khodi, Mohamad Javad Bagheripour Diphtheria is a fatal disease caused by exotoxin of Corynebacterium diphtheria. This toxin consists of two chains, catalytic chain (A) and binding (B) chain. By binding chain (B), the toxin binds to its receptor on numerous body cells such as myocardial, kidney and peripheral nerve cells. After entering, catalytic chain (A) inhibits protein synthesis and finally can cause cell death. At this time, the toxoid form of diphtheria toxin is used as vaccine. The aim of this study was the immunological analysis of the mutated synthetic catalytic subunit of diphtheria toxin in laboratory animals as a vaccine candidate, in addition to polyclonal antibody production and purification against diphtheria toxin. For this purpose the Dtx recombinant protein (with two mutant: A158G and G52E) was expressed using pET28a/DtxA plasmid in E. coli Bl21DE3 host. Then, recombinant protein, as a candidate vaccine, was extracted and purified. After evaluating and confirming the protein by SDS-PAGE and western blotting, immunization carried out in laboratory animals. Finally, followed by antibody titration by ELISA, antibody purification performed as well.The mutated recombinant protein prepared from an optimized expression was extracted and purified. Then, this protein was confirmed by SDS-PAGE and western blotting. ELISA results showed a satisfactory immunization of animals by this protein. Polyclonal antibody production and purification against diphtheria toxin was performed by G protein column and confirmed by ELISA. ELISA results showed a high titer of polyclonal antibody against diphtheria toxin in animal's serum after immunization by recombinant DTx protein.
Rapid Detection of Vibrio Cholerae by Polymerase Chain Reaction based on Nanotechnology published in

Journal of Applied Biotechnology Reports

Published by Baqiyatallah University of Medical Sciences
Gholam Reza Herfehdoost, Mahdi Kamali, Hamid Reza Javadi, Davood Zolfagary, Ali Choopani, Bahareh Ghasemi, Saeideh Hossaini Quick identification of Vibrio Cholerae in epidemics is important, on the other hand; conventional methods are time-consuming and costly. The aim of this study was to develop a rapid, inexpensive and high sensitivity method for quick identification of Vibrio Cholerae. For this purpose we designed a PCR detection based on magnetic nanoparticles for identification of bacterial DNA by PCR Dynabead. So we used the biotinylated Probe for binding to DNA extracted from Vibrio Cholerae and other bacterial species (Salmonella, Shigella, Pseudomonas, E.coli) Using magnetic bead isolated with magnetic field, the Vibrio genome-specific primers (HlyA) for pathogen detection (PCR) was used. The results showed specific band was just for Vibrio Cholera (PCR positive); therefore designed probe was specific for Vibrio Cholerae. According to the findings, this study is characterized the high sensitivity of PCR using biotin-containing probes for DNA of Vibrio Cholera in contrast to the traditional methods.
Religious Orientation and Academic Stress Among University Students published in

Published by
Bhat SA The present study examined the extent to which religiosity, operationalized as intrinsic and extrinsic religious orientation was related to academic stress in self-report measures among 100 (40 male and 60 female) students from Kashmir University. Pearson’s product method was used to find the results. The results indicated that intrinsic religious orientation has a significant negative relation with facets of academic stress such as group study stress, time management stress and stress due to peers. In other words it can be said that more intrinsic the person is lesser the amount of academic stress he faced. Similarly a positive correlation was found between extrinsic religious orientation and five of the seven facets of academic stress namely, Result Stress, Group Study Stress, Peer Stress, Time Management Stress and Self Inflicted Stress. In other words it means that more an individual is extrinsic higher the scores are in academic stress facets mentioned above.
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