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Annals of Tropical Medicine & Public Health

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1 Comparing the techniques of laminectomy , Abdulnaser Abdulqader Salih
CL- shortcut for classical laminectomy- is the standard lumbar spine stenosis (LSS) treatment and succeeds in 60.87% of patients. The goal of our study is to compare the clinical results of Transspinous- Split Laminectomy TSSL, which reduces paraspinal muscle destruction, and protects the alignment of vertebrates. The data consist of 50 patients which are divided to group A with 25 patients who treatment via transspinous split laminectomy and group B with 25 patients who treatment via classic laminectomy. The two groups were administered by the same surgeon and the process technique is chosen randomly. The sample does not contain pregnant patients and does not have any infectious diseases, malignant infections, previous treatment of spinal fractures or disc hemiation, and they have previous injury in the treatment of iatrogenic, spondylosis, spondylosis or radiation-documented instability.  The incision lenght and amount of hemorrhage were matched to group A and group B. The length of the incision was shorter in group A. Avoided circumference dissecting muscles in group A reduces the amount of hemorrhage. Paraspinal muscle atrophy (PMA) rating was lower in group A. The minimal muscle atropy is scheduled during dissection of minimal muscular atrophy in TSSL technique. The reduction of the values of postoperative Oswestry pain grade of patients compared to another group clinically verifies that adequate decompression has been alleviated. The smaller size of the incision, the less bleeding during the operation and the injury of the paraspinal muscle results in less pain after the surgery, and thus recovering faster. The minimally invasive method (TSSL) is a good alternative to the conventional of the traditional laminectomy.
2 Development of X-ray application in cancer treatment and the use of medical imaging techniques , Marwa H Abbas
Role of radiotherapy in palliative care of cancer patients. Radiotherapy plays an important role on palliative care of cancer patients. This type of therapy accounts for about 30 to 50% of all radiation delivered in a medical ward. The goal is to improve the patient's quality of life by providing rapid relief of symptoms which depends of each specific localization. The cause of the symptoms must be carefully identifed (agreement between radiological and clinical findings) in order to establish the precise target for radiation. Radiation dose and fractionation should be adapted to the patient's general status and expected duration of life in order to limit the duration of treatment and secondary effects
3 Correlation of FNAC lymph node cytology with CD4 count in HIV seropositive adults , Mukherjee Sumana, Mukhopadhyay Keya, Bhattacharya Pranab
Context: Lymphoid tissues are common targets of HIV infection. FNAC is the initial investigation of choice in these cases. Aim: To evaluate the usefulness of FNAC in HIV positive lymphadenopathy in our center Methods and Material: FNAC was performed in 155 HIV positive patients presenting with lymphadenopathy. Smears were stained with Giemsa, ZN, and PAS/Grocotts/PAP according to cytological findings. Statistical analysis: The data was analysed using the T Test. Results: Tuberculous lymphadenitis was the most common diagnosis (44%). Smear positive was found in 29% cases. Necrotizing granuloma and smear positivity was signficantly higher in cases with CD4 count less than 200. Reactive hyperplasia was significantly higher in the CD4 less than 200 category Conclusion: FNAC is very useful and gives specific diagnosis in most cases of HIV lymphadenopathy. Lower CD4 count significantly increase the smear positivity for AFB.
4 RnBeads 2.0: Comprehensive analysis of DNA Methylation Data , Methaq Hadi Lafta, Lekshmi Gangadhar
DNA methylation is a widely investigated mark with importance roles in development and disease. High-throughput assays enable genome-scale DNA methylation in large number of samples. Here, we desribe a new version of our RnBeads software-an R/Bioconductor package that implements start-to-finish analysis workflows for infinium microarray and various types of bisulfite sequencing RnBead 2.0 (https://rnbeads.org) provides additional data types and analysis methods, new functionality for interpreting DNA methylation difference, improved usability with a novel graphic user interface, and better use of computational resources. We demontrate RnBeads 2.0 in four re-runnable use cases focusing on cell differentiation and cancer
5 Dental Ergonomics: Awareness and practices among dental undergraduates in Saudi Arabia , Zakirulla M, AmerahBedah Muraih, Amjad Ali Al-Whlan, Ohood Saeed Al-merei, Rasha Hussain Al-zahrani, AbdulazizBedahAlshahrani
Aim: The present study aims to assess knowledge, practices, and conditions of work place regarding ergonomics among dental students in abha city, Saudi Arabia. Materials and Methods: A cross-sectional study was carried out on the sample size of 156 dental students (Males 91 & Females 68) from 5th, 6th year and internship year of the BDS program (Students involving clinical courses) in College of Dentistry, King Khalid University, Abha, Saudi Arabia. The survey was close ended, self administered questionnaire that focused on various positions while working was used as the data collection method. They survey was collected and organized into Microsoft Excell spreadsheets (Microsoft Inc., USA) , and was statistically analyzed utilizingg the Statistical  test used here was the chi-square and P vales less than 0.05 were considered to be statistically significant. Results: When studnets were asked about this question, 92% of students agreed with sitting dentistry, 5% practised standing dentistry, and 3% were not sure. Student's interest in learning and exercising he correct working posture: 93% of students said yes. Majority of students were interested in learning the right working posture. When the students were asked about the importance of proper posture in dental clinics, 90% of students said yes. Conclusion: Findings confirmed that there is a positive awareness of ergonomics among dental students. There is no statistical difference between males and females in knowledge and behaviour related to ergnomic principle among dental students.
6 Safety Disposal of Electrophoresis Gels and PCR contaminate with Ethidium Bromide and alternative methods , Methaq Hadi Lafta, Amal Raqib Shamran
Electrophoresis gel is commonly used in molecular biology laboratories for the identification of nucleic acid (DNA or RNA) and proteins. This gel typically be agarose-based. This electrophoresis process utilized an organic fluorescence dye or inorganic such as silva ( which is EPA regulated material) to stain DNA or protein. Waste of the DNA identification process much be managed and disposed in a manner to protect public health and the environment. Purpose: To ensure safe, prudent disposal as well as reduce the amount of hazardous waste Material: This can be accompanied by choosing less toxic materials and work practical methods that minimize the quantity of waste generated as well as the toxicity of the waste material itself. In cases where safer materials or work practices cannot be employed, waste collection methods and regulatory agency requirement are followed. Background: There are a number of different protocol and alternative dyes used in the preparation of electrophoresis gels. Gels can be cast with or without dyes. The DNA/proteinc can be stained by adding the dye to the sample before electrophoresis; the gel can be placed in a dye solution after electrophoresis has been completed. Waste management: Waste disposal requirement will vary depending on the dye used and the methodology used to stain the cells. The electrophoresis waste of the DNA or protein product identification process must be managed and disposed in a manner that is consistent with the committment it has to protect public health and the environment. The following provides Lab managers/ generators with the proper procedures for managing and disposing electrophoresis-assocated gel wastes
7 In vitro Chick chorioallantoic membrane study of chitosan capped 5-fluorouracil conjugated gold nanoparticles , Akhila Rajan, PK Praseetha, Methaq Hadi Lafta, VN Ariharan, ST Gopu Kumar
Introduction: Chitosan is a linear polysaccharide composed of randomly distributed ß-(1→4)–linked D–Glucosamine (DE acetylated unit) and N–D–Glucosamine (acetylated unit). It is made by treating the chitin shells of shrimps and other crustaceans with an alkaline substance, like sodium hydroxide. Chitosan has a number of commercial and possible biomedical uses. Chitosan's properties also allow it to be used in transdermal drug delivery. Aim: The aim of the work is to deliver therapeutic compound to the desirable site in the treatment of diseases. Methods: Chitosan nanoparticles (NPs) was prepared using the ionin deletion method. 5' Fluorouracil (5'FU) loaded Chitosan nanoparticle (5FCN) was synthesized and tested for its angiogenic activity on  cell lines by a chick chorioallantoic membrane (CAM) assay. ConclusionL The angiogenic activity of 5-FU loaded chitosan nanoparticles provided a platofrm for treating cancer with biopolymer nanomaterials. The combination of nanoparticles with Chitosan in the form of Nano composite matrices provided the highsurface area required to achieve a high loading of enzymes, drugs, and a compatible micro-environment to facilitate stability
8 Application: Coating of Nanoparticles to minimizing harmful environmental pollution by chemical fungicide , Rana Ibrahim Khaleel
Field experiment carried out for evaluationusing nano-silver solutions to reduce environmental pollution by fungicide. Four concentrations of AgNPs (100 ppm, 50 ppm, 25 ppm, and 12.5 pmm) were prepared by diluting the original stock solution with distilled water. From the concentration were ready used in coated orange fruit compared with fungicide and uncoated fruit as control treatment. The fruits were kept in box carton (40x40x30cm) and incubated under room conditions for 28 days to study the formation to green mold and black fungal and shelf life for fruit stored. The result showed high infection recorded in uncoated fruit compared with other treatment with values reached ( 86.66 and 91.1) for green modl and black respectively, while ppm recorded completely inhibition infection (0%) for fungal decay index. Also coating fruit with 100 and 50 ppm recorded significant different reached (3.8 and 3.9%) after four weeks from storage. Nanotechnology applied could play a fundamental role for this purpose and research in packaging fresh fruit and minmized environmental pollution by chemical fungicide
9 Genetic detection of hepatitis B virus by PCR technique among children under 7 years in Hilla city, Iraq , Amal Raqib Shamran, Tsahel H Al Dulaimi, Ammar S Khamis, Rula Dhahir Abdulmohsin
Hepatitis disease is mean the inflammatory and destruction of the liver. Its commonly caused by viral infection, Hepatitis B Virus (HBV) is one of the main resone of liver destruction, it spread when people come in contact with the blood, open sores, or body fluids of someone who has the (HBV), 3.5% of global population is chronic infected with HBV although the incidence of HBV infections is decreasing owing to vaccination and to a lesser extent. Hepatitis is a serious public health problem distressing many of people worldwide. Inadequate data is available on this issue in Iraq. This study was carry out with the aim of determining the genetic sequencing in human and risk factors of hepatitis Bvirus (HBV) among the general population and among blood donors. Methods: Blood samples from volunteers; have been detected the hepatitis-gen by PCR ,we see that the 48 out of 250 children patients ( 19.2% ) among 5months to 5 years , in this study population the biggest percentage were 45.83 % in aged 3-5 years while the second percent was 31.25 % in aged month-1year,while it was 12.5% in age 1-3 years and 10.41% in age 5-7 years, table (1) ,in this study we see that male were 22 out of 48 ,45% while the femal were 26 out of 48 ,55%.
10 Comparison of APGAR Score in neonates born after elective spinal versus general anaethesia , Idrees Jameel Khalaf
BACKGROUND: ten point Apgar score has been used to compare the effect of spinal anesthesia versus general anesthesia on Apgar score of neonates born by elective caesarean section. STUDY DESIGN: Randomized prospective study. SETTING: This study was conducted in the Department of pediatrics-Haweejha general Hospital/Kirkuk/Iraq., between December2017toJune2018. SUBJECTS AND METHODS: In this study was achieved on sixteen women bestowing for Elective lower segment caesarean section. Thirty mothers were given general anaesthesia and other 30 mothers received spinal anaesthesia. The Apgar score was recorded at 1 minute and 5 minute interim after each delivery. RESULTS: Out of thirty mothers, who received general anaesthesia, 25 patients (82.3%) give birth to neonates having Apgar score ≤ 6 at one minute after birth and the remaining 5 neonates (17.7%) had Apgar score of ≥7. On the other hand out of 30 mothers who received spinal anaesthesia only 10 mothers give birth to neonate having Apgar score ≤ 6 at one minute after birth, who improved at 5 minutes interval, and their Apgar score were ≥7.It had been found that those neonates who were born under G.A were ten folds more likely to have Apgar score less than or equivalent to 6 at first minute compared to those with spinal anaesthesia, the odds ratio=10 and 96%assurance interval of the odds ratio (2.95-35) and p=0.00025 which is highly significant, G.A had greater risk on newborn at the first minute.
11 Frequency of norovirus in Egyptian children with gastroenteritis and coninfection with rotavirus , Nabil Abd El-Aziz Mohsen, Ayman Emil Eskander, Azza Ahmed Aboul Enein, Carolyne Morco Ibrahim, Mohammed Ali Abd El-Hafiz
Pediatric gastroenteritis is a major cause of childhood mortality and morbidity worldwide, especially in developing countries. Diarrhea can be caused by a variety of different pathogens including bacteria, viruses and parasites. Among the viruses; rotavirus has been extensively studied. As the rotavirus vaccination coverage improves, the causative agent’s shift may be more towards the Calciviruses (Norovirus, Sapovirus). The present paper aimsmainly providing useful epidemiological data on the gastroenteritis associated with norovirus and rotavirus among Egyptian children aged less than five years and to determine the frequency and confection with these two viral agents. In the present paper, and from Jan., 2016 to Dec., 2016, a hundred stool specimens obtained from Egyptian children of age less thanfive years had been attended the outpatient clinic of pediatric gastroenterology unit of Children Hospital, Cairo University were tested for rotavirus and norovirus using enzyme-linked immunosorbent assay. The results showed that the isolated Rota virus infection was 38%, isolated norovirus infection was 7% and the combined norovirus and rotavirus confection was 5%. 67.5% of rotavirus cases were males and 72% of cases aged less than one year while 58% of norovirus were females and 58% of cases aged above one year. Therefore, rotavirus is still the most frequent cause of acute viral gastroenteritis in Egypt. In spite of low prevalence of the norovirus, it is found in Egypt and is considered the second most frequent cause of viral gastroenteritis. Perhaps with time and improvement in the means of investigations, the real norovirus prevalence in Egypt may be elucidated.
12 Resistance of Trypanosoma congolense forest isolates to isometamedium chloride and diminazene aceturate in West Democratic Republic of Congo , Kabamba MW, Sumbu J, Lombe B, Tsilenge G, Badibanga D, Natoro C, Telamanu E, Nsalambi S, Malekani J, Mamoudou A, Pyana P, Domy P, Masumu J
Resistance to trypanocides is increasing rapidly in sub-Saharan African countries where African animal trypanosomiasis occurs. An investigation was carried out over a four-year period to assess trypanosome resistance to trypanocides in the western Democratic Republic of Congo (DRC) using the standard mouse test. Eight strains of Trypanosoma congolense Forest, two from Kimwenza and one from N'Djili Brasseries in the City of Kinshasa Province, three from Mbanza Ngungu in the Province of Central Kongo and two from Mushie in the Province of Mayi Ndombe, were isolated from pigs and cattle. Following the amplification in mice, 105trypanosomes of each strain were inoculated in three groups of six NMRI mice. Twenty-four hours later, the first group was treated with 1g of isometamidium chloride (ISM)/kg BW/mouse, the second group was treated with 20mg of diminazene aceturate (DA)/kg BW/mouse while the third group was left untreated and served as control. All these mice were followed up for the development of parasitemia for a period of two months. At the end of the experiment, only the N'Djili Brasseries strain was resistant to ISM at a dose of 1 mg / kg bw. When multidose test was applied, no resistance was observed in groups of mice treated with high doses (from 2 to 20g ISM/kg bw/mouse). This study shows for the first time the occurrence of trypanosome resistance strains to ISM in DRC. The DRC becomes the 20th sub-Saharan country of the chemoresistance of trypanosomes to trypanocides has been reported.
13 Hemophagocytic lymphohistiocytosis in a Nigerian child: A review of the literature, Adaobi Uzoamaka Solarin, Montunrayo Oluwabukola Adekunle, Rita Obiageli Obaze, Omodele Oluwayemisi Jagun, Daniel Ogbaro
Hemophagocytic lymphohistiocytosis (HLH) is a rare, life-threatening, underdiagnosed hematologic disorder that occurs from unregulated immune activation, extreme inflammation, and tissue damage. The major cause of mortality in HLH is a delay in diagnosis and prompt initiation of treatment which occurs due to the variability of the clinical manifestation of this disease entity. We present a case of a 17-year-old adolescent who presented with fever, painful facial swelling that partially resolved with the initial use of intravenous dexamethasone. Diagnosis of HLH was finally made and he had complete resolution of symptoms following appropriate treatment. With careful search, there is no report of HLH in Nigeria and the possibility of missed diagnosis and underdiagnosis cannot be ruled out. This case report of HLH is the first in Nigeria and due to the rarity of the disease the need to raise more awareness is imperative as a high index of suspicion is required for diagnosis.
14 Changing Trends of lower respiratory tract pathogens: A five year study , Shenoy Padmaja A, Purandare Mrughandha K, Chawla Kiran
Context: Lower respiratory tract infections (LRTIs) are one of the most common problems encountered by physicians posing huge impact on health care system. Among the diverse respiratory bacterial etiological agents, Gram-negative bacilli predominate in developing countries. Antimicrobial resistance among the respiratory pathogens is a raising concern leading to prolonged hospital stay and high morbidity/mortality. Aims: The aim of the study was to determine the common bacterial aetiological agents causing LRTIs and to study their changing trends of antibiotic resistance pattern. Settings and design: Retrospective study was conducted from January 2012 to December 2016 in Department of Microbiology, tertiary care teaching hospital, Manipal. Methods and materials: Respiratory specimens including Broncho alveolar Lavage (BAL), Endotracheal (ET) aspirate and sputum samples were processed for Gram stain and culture. The respiratory pathogens were identified and were subjected to antibiotic susceptibility testing following standard techniques. Statistical analysis used: Obtained results were analysed by SPSS version 16. Changing trends of antibiotic resistance was studied by linear regression. Results: Of the 22095 samples received, significant bacterial growth was observed in 6766 (30.6%) specimens. Gram-negatives (5581, 82.5%) outnumbered the Gram- positive isolates (1156, 17%). Among the Gram-negative bacilli (5581, 82.5%), non-fermenters were (3297, 59%) predominantly isolated followed by members of Enterobacteriaceae (1622, 29%). Among the non-fermenters, Acinetobacter baumannii was the frequent isolate (1696, 51.4%) showing significant increasing trends of resistance to cefoperazone-sulbactam (R2= 0.85 and P value 0.03) and maximum increase in antibiotic resistance was observed in the year 2016 (77%). Conclusion: Significant upsurge in the resistance trends of common antibiotics in LRTIs calls for judicious usage of antibiotics & strict infection control practices.
15 A descriptive cross-sectional survey among the International Islamic University Malaysia’s student on e-waste generation and public health problem in Malaysia, AK Azad, MA Islam, NK Sunzida
Objective: The main aim of the survey was to assess the respondent’s opinion on the factors influencing the generation of e-waste and its impact on public health in Malaysia. Methods: The research project involved a cross-sectional survey of International Islamic University’s students conducted in 2017 at Kuantan campus in the Pahang state, Malaysia. Along with info on demographic individualities, data were collected on several factors (agreement with questionnaire items regarding) using a 4-point Likert scale, with 4 representing “agree”, “strongly agree”, “disagree” and “strongly disagree,” respectively. Results: A total of 296 students completed the questionnaire. The age group of 18-22 covers 46.96% of all respondents and 61.82% was females. In ethnicity of respondents, 86.82% Malay and 30.07% were from rural area and rest of them were urban area. The volume of e-waste is raised due to the excess supplies of electrical appliances (71%), update their devices (65%.), additional electrical devices (59%), keeping the unused electrical appliances (71%), wholesalers selling bad quality appliances (45%), mechanic should not add and drop of their devices (71%). Regrettably 83% of the respondents doesn’t have appropriate knowledge of e-waste. 51% students agreed and 38% strongly agreed that there is no noticeable campaign on e-waste for public awareness. According to the 93% students’ opinion, individuals, the suppliers, industries and even the institutions are not aware to reduce e-waste. But a little 20% think that government does provide facilities for electrical device disposal. In public health perspective, e-waste contains a lot of hazardous chemical (strongly agreed 77% and rest 23% agreed). Fortunately, the serious health hazards led by e-waste (86% strongly agreed and 14% agreed). E-waste leads to infertile land, cancer, lung diseases and so others strongly agreed 70% and agreed 22% respectively. Conclusion: Findings suggest to organize the awareness programme in institutional, organization and educational institutional level to reduce generation of e-waste and its risk.
16 Clinical and Functional Parallels of severe uncontrolled ashtma in children , Natalya L. Potapova, Inessa N Gaymolenko
Context: Over the past 20 years in the Russian Federation the prevalence rate of asthma symptoms was 5.3% in 13- 14 year-old adolescents and 4.7% in7-8 year-old children. Lack of control or partial control over the symptoms of the disease is recorded in more than half of the patients and is the risk of adverse outcomes. The purpose is toidentify the markers of severe uncontrolled progression of bronchial asthma in children. Place, time and rationale: The study was conducted in the Regional Children’s Clinical Hospital of Chita in 2015-2017. Materials and methods: In total 112 children aged 1 to 18 years were surveyed. For an in-depth study, 68 children with wellcontrolled asthma and 32 patients with uncontrolled asthma progression were selected. The statistical analysis used: Comparison of independent groups by the Mann-Whitney test, Spearman’s correlation coefficient and Cramer’s contingency coefficient, calculation of the odds ratio (OR) were used as statistic processing techniques. Differences were considered statistically significant at p
17 Association betweeen ABO blood group and Diabetes Mellitus , Khansaa A Ibrahim Albaroodi, Zahraa S Hatef, Basheer Akeel Al-Ali, Shuaib AlAhmed
Background: Many epidemiological studies discussed the linkage between ABO blood group and the risk of developing diabetes mellitus. Aim: This study aimed to evaluate the association between ABO blood group and diabetes mellitus. Methods: This study was a cross-sectional study, conducted at endocrinology and Diabetic center in AL-Hussein Teaching Hospital in Karbala, Iraq. Participants were randomly selected and assigned to either healthy group or to patients with diabetes group. The study was carried out from November 2018 until January 2019; The collected data were analysed using the SPSS (version 20) software package (SPSS Inc., Chicago, IL).Results: 244 participants were included in the study, 157 of them were diabetic patients and 87 subjects were healthy. Non -significant association between ABO blood group and type 2 diabetes where the p value was > 0.05. However, significant differences between blood groups in terms of Fasting Blood Sugar were found; and participants with blood group AB+ have strong relation with Fasting Blood Sugar. Conclusion: Findings of this study suggested an existing relationship between blood group AB+ and Fasting Blood Sugar; but, these findings was not confirmed by a significant association between diabetes mellitus and ABO blood groups that raise a need for future researches to be done.
18 Pllatelet-rich Autoplasma effect on intestinal anastomosis regeneration in  rabbits , Berik Nuraddinovich DZHUMABEKOV, Auyeskhan DZHUMABEKOV, Dzhumagaly Kazybaevich ISMAILOV, Talgat Alishovich BAITILEUOV, Ildar Rafisovich FAKHRADYIEV
Background: The failure of the intestinal anastomosis remains one of the main problems in gastrointestinal surgery. Modern principles for solving this problem are focused on the cellular level of regeneration using platelet-rich plasma (PRP). In this study, we evaluated the effect of PRP and experimentally compared the effectiveness of various application methods in intestinal anastomosis regeneration in rabbits. Materials and Methods: Platelet-rich autoplasma was obtained from 81 rabbits, which were used to study two methods of applying PRP (injection into the muscle layer / soaking in the intestinal wall) in comparison with the control group on the end-to-end small-intestinal anastomosis. A morphological study of intestinal anastomoses was performed after 24, 48, 72 hours, as well as in 7 days. Evaluation of deformation and strength characteristics of the anastomosis was carried out on the 7th day. Results: Analysis of the histological and morphometric data of the study showed that the regeneration between the samples of intestinal anastomosis treated with PRP soaking and injections into the intestinal wall is more favorable in comparison with the control group, which, in turn, was determined by the quantitative ratio of inflammatory infiltrate, fibroblast proliferation, neoangiogenesis, and collagen deposition. PRP soaking in the intestinal wall significantly increased the number of adhesions battles. The resulting conglomerate adhesions contributed to severe deformation and narrowing of more than ½ of the lumen in the intestinal tube. Analysis of the deformation and strength characteristics of the formed anastomoses also showed significantly high values of the rupture strength of the anastomoses treated with PRP, in contrast with the control group. Conclusion: PRP soaking and injection into the intestinal wall can have a positive effect on the regeneration of the intestinal anastomosis. PRPinjection into the muscular layer of the intestinal wall significantly reduces the development of adhesions in comparison with the PRP-saturated group of intestinal anastomoses. Further research is needed to clarify the optimal method of PRP application, which will improve the regeneration of the intestinal anastomosis.
19 Assessment of left ventricular function by echocardiography in sample of Iraqi patients with lymphoma treated with doxorubicin , Abbas Al-Sharifi, Hayam Taher Faisal
Background: Lymphoma is one of the most common hematological malignancies, which can be treated effectively by Anthracyline –based chemotherapy. Early detection of subclinical Doxorubicin cardio toxicity is a continuing challenge in clinical oncology. Methods has been done to minimize cardiotoxicity include serial monitoring of cardiac function or empiric doxorubicin dose limitation. Objective of study: Detection of cardiac effect of doxorubicin in patients with lymphomaand determine the relationship between cumulative dose of anthracylcline and late cardiotoxicity Patients and methods: 50 patients (40 patients with Non-Hodgkin lymphoma and 10 patients with Hodgkin S lymphoma) were enrolled in prospective hospital based study in Hematological disease center and Baghdad Medical city, from 1st of May 2016 till 1st of September 2016, their age ranged from 16 years to 70 years old .All patients had been treated accordingly either by CHOP (C ; Cyclophosphamide. H ; Doxorubicin Hydrochloride O, Vincristine, Prednisolone) or by ABVD regimens (A: adriamycin (doxorubicin) B; bleommycin; V; vinblastine D;dacarbazine) .Patients were examined by Echocardiography, looking for cardiac dimensions ,ejection fraction and pulses-wave Doppler for any left ventricular diastolic dysfunction.Cumulative dose of Doxorubicin ,sex age ,type of lymphoma .smoking and duration of treatment were evaluated as a potential risk factors for the development of cardio toxicity. Results:Total of 50 patients collected their age ranged between 16 and 70 years old with mean of 48.8 ± 15.54 years , there were 21(42%) male and 29(58%) female , 10 patients had Hodgkin’s lymphoma, treated by ABVD regimens and 40 patients had Non-Hodgkin’s lymphoma,they were treated by CHOP regimens.The duration of the treatment varies between one year to 11 years of treatment with a mean of 4.4 ± 2.66 years duration, the total dose of Doxorubicin ranging from 400 – 680 mg/m2 with a mean of 554 ± 89.45 mg/m2.Of the 50 patients,28(56%) developed LVH, 24(48%) developed left ventricular diastolic dysfunction and 12(24%) developed left ventricular systolic dysfunction.Significant correlation was found between the ejection fraction and dose of the doxorubicin ( p value < 0.01) .Significant correlation between the type of lymphoma ( p value < 0.001) , duration of treatment ( p value 0.001) and the dose of Doxorubicin(p value 0.012) with development of LVH also has been noted Conclusion The incidence of cardio toxicity is significant in lymphoma patientstreated by one of regimens that contain doxorubicin.The incidence is related to the dose of doxorubicin.The longer period of treatment with doxorubicin the higher chance to get cardio toxicity
20 The relationship of serum Cyclophilin-A level with Glycated haemoglobin and its role in the early prediction of coronary artery disease in Iraqi patients with type 2 Diabetes Mellitus , Dunia Tahseen Nema Al-Aridhi, Zainab A Razak Al-Sharifi, Muthanna Al-Quraishi, Khalid I H Allehibi
Background Cyclophilin-A is a protein, secreted from the endothelial cell and vascular smooth muscle cells in response to the reactive oxygen species (ROS),it has peptidyl-prolyl cis-trans isomerase activity (PPIA).It is a novel biochemical marker, which is associated with type 2 diabetes mellitus (T2DM) and coronary artery disease (CAD).Type 2 diabetes mellitus is a heterogeneous syndrome affecting many people in the world; it has a variety of complications that lead to serious problems such as coronary artery disease (CAD). T2DM can be diagnosed efficiently by glycated haemoglobin (HbA1c), which reflects the state of blood glucose in last three months, so it is considered as a good indicator of long-term glycemic control according to the recommendation of the American Diabetes Association (ADM). Objective To assess the correlation of Cyclophilin-A with the HbA1c, and to detect the role of Cyclophilin-A in the metabolic complications of T2DM, like coronary atherosclerosis, and to evaluate the reliability of this marker in prediction and identification of CAD early in type 2 diabetic patients when the preventive measures and treatment facilities are effective. Methods One hundred sixty-one volunteers aged (40-60 year), and their body mass index (20- 25Kg/m2). They were divided into six groups [two control groups (Ia, Ib), two diabetic groups (IIa, 1Ib) and two coronary artery disease groups (IIIa, IIIb)]. These volunteers were diagnosed by endocrinologist and cardiologist, they are distributed among the groups as follows: [(Ia = control (neg. cath. without DM, 28 persons), Ib = control (apparently healthy, 25 persons), IIa = DM (neg. cath., 30 person), IIb = DM (depending on history, 25 person), IIIa = CAD (without DM, 20 person), IIIb = (CAD + DM, 33 persons)]. The parameters that measured in this study were (plasma glucose, Glycated haemoglobin, serum lipid profile and serum Cyclophilin-A, all these parameters were measured in the fasting state for each group. Also, the relationship was found between Cyclophilin-A and Glycated haemoglobin for [total control groups, total DM groups, CAD without DM group, and CAD with DM group]. Results Cyclophilin-A levels were a significant elevated in (IIa, IIb, IIIa and IIIb ) groups as compared with control groups (Ia, Ib), and the highest level is showed in CAD+ DM group (IIIb). Moreover, there is a significant positive correlation between serum Cyclophilin-A and HbA1c in the [total DM groups (IIa +IIb), P =6.74E-09, r = 0.687] and in[CAD + DM group (IIIb), P =0.00369, r = 0.491], but the results did not show a significant correlation in the [total control groups (Ia+Ib) and CAD without the DM group (IIIa)].The present findings showed significantly elevated in the means of FPG level, HbA1c, serum cholesterol level, in groups (IIa, IIb and IIIb) as compared with control groups (Ia, Ib) except in (IIIa) group there was no significant difference. In addition, there was a significant increase in the mean serum levels of triglyceride, VLDL and LDL for (IIa,IIb, IIIa and IIIb ) groups than in the control groups. While there was a significant decrease in mean serum level of HDL for groups (IIa,IIb, IIIa and IIIb) than in the control groups (Ia, Ib). Besides, there was a significant decrease in serum HDL level in CAD groups (IIIa, IIIb) than in DM groups (IIa, 1Ib). Conclusion The highest level of serum Cyclophilin-A in diabetic patients with CAD and its positive relationship with HbA1c can indicate the role of Cyclophilin-A for predicting CAD in type 2 diabetic patients.
21 Food poisoning: Bacterial isolation and knowledge regarding food-handle with Anti-Haemolytic Activity of vinegar , Nada Khazal Hindi, Zainab Adil Ghani Chabuck, Thikra Adnan Jawad Banimuslem, Jameel Ali Kadhim
Food poisoning is a sickness that is due to consumption of water or food contaminated with bacteria themselves and/or their toxins, or with viruses, parasites, or because of chemicals. the cross-sectional study of 100 food-handlers by conducting face to face interview by questionnaire, and take samples from food, tools and workers, at November 2017 to March 2018 in Al-Hilla city, to get information about the practices, attitudes and knowledge of the food-handlers regarding food safety. Antimicrobial and bacterial haemolytic activities were evaluated before and after the usage of vinegar. The commonest category of age group among food workers was 21-30 years (55%). The highest percentage of bacterial isolated from restaurants and workers were S. epidermidis, S. aureus followed by E. feacalis and E. coli. The educational level indicated that the largest groups among workers (41%) have only primary education. The 93% of workers have no learned or enter food safety courses. Most cases of food poisoning (about 50% of the total cases) were attributed to improper handling of food by food workers. Vinegar inhibits bacterial growth and haemolysis at concentrations 30% and 40% but not at 10% and 20%. Young workers do not have a good level of education or enter food safety courses. Most bacterial isolates were found on the skin, nose and gastrointestinal tract which indicates their responsibility on food poisoning cases due to poor personal hygiene of the workers, and poor usage of hygiene protective devices. Also vinegar showed effective antimicrobial and antihaemolytic actions that can be used as an antiseptic by its addition to food equipments during washing and cleaning.
22 Knowledge assessment of female students of Southern Technical University in Basrah-Iraq, regarding breast cancer and breast self-examination , Majid Abdulwahab Maatook, Abdul Kareem M Jawad, Rajaa Ahmed Mahmoud
Background: Globally, epidemiological statistics provide an evidence that breast cancer is the second common type of cancers and definitely the most common type among women with a fraction of 25% of the total registered cancer cases.And by reviewing the worldwide mortality rates, it is among the top ten killers worldwide with an estimated increase in the number of deaths from 7.4 million in 2004 to 83.2 million in 2015. With such an increasing burden of the disease over time, a huge necessity emerged to conduct more studies on breast cancer knowledge, early detection practices, in addition to the assessment of the disease risk factors and preventive measures. The study objective: To assess the knowledge of female students of the Southern Technical University in Basrah regarding breast cancer (signs, symptoms, breast self-examination and ways of prevention). Methodology of the study: It is a cross-sectional study that included 250 female students of the Southern Technical University in Basra with the age between 18-24 years during the year 2018. Results: The study found that 66.8% of the female students know some or all the signs and symptoms of breast cancer compared to 33.2% of them who did not know any sign or symptom of the disease. 62.8% of the students included in the study did not know that breast cancer is a preventable disease. More than half of the sample (55.6%) did not hear about breast self-examination. In addition, the majority of those who knew about breast self-examination mentioned that the importance of breast self-examination is early detection of breast cancer. While the rest of the study population thought that it is important only for reassurance. Conclusions and recommendations: The study concluded that more health education training and awareness sessions on breast self-examination technique should target students at an early age with a special focus on university students.
23 The Antibacterial Effect of bioactive compounds extracted from Cassia didymobotrya (Fresenius) Irwin & Barneby against Some Pathogenic Bacteria , Hussein J Hussein, Nebras Mohammed Sahi, Ali Malik Saad, Huda Jasim Altameme
To detect the impact on some gram negative and gram positive pathogenic bacteria of crudely extract phenolic, alkaloid and terpenoid compounds of Cassia didymobotrya.Cassia didymobotrya antibacterial action was achieved in vitro by agar well diffusion methods against certain gram negative and gram positive bacterial pathogenic bacteria .Results showed that active Cassia didymobotry compounds have a large variety of antibacterial actiontoward negative gram and positive gram bacteria.This investagation shows that we could assume the impact of bioactive compounds on different pathogenic forms of life at different concentrations in cassia didymobotrya.Cassia didymobothria leaves alkaloids were shown to have increasinglypowerful antibacterial action for Escherichia coli, Pseudomonas aeruginosa, Streptococcus mitis and Lactobacilus acidophilusexcept Staphylococcus aureus. While terpenoid compounds it was increasingly powerful to all except Lactobacillus acidophilus, but Staphylococcus aureus and Streptococcus mitis completely resistant to phenolic compounds.
24 Prevalence of lung cancer in non smoker patients attending Al-Diwaniyah Teaching Hospital , Thimar Malik Khudhair, Hassan Raji Jilla'ab
Background: Smoking is a well known risk factor that is implicated in a number of human malignant disorders. Aim of the study: To study the prevalence of lung cancer in a sample of Iraqi non smoker patients and the main risk factor implicated in such malignant tumor. Patients and Methods: In this hospital based study, in order to explore the prevalence rate of bronchogenic carcinoma in non smoker patients, it retrospectively collected data about those patients for the last four years, starting from January 2016 through June 2019. This information was retrieved from patients’ records that are already present in oncology and respiratory units in Al-Diwaniyah teaching hospital, Al-Diwaniyah province, Mid-Euphrates region of Iraq. The following variables were included in the questionnaire form: Age, gender, occupation, education level, residency, socioeconomic status, marital status history of smoking, history of second hand smoking, number of household smokers, type of smoking, history of alcoholism, history of chronic illness, the main presenting clinical features and clinical features of carcinoma and finding in examination as well as those obtained from investigations. Results: In the current study, the prevalence rate of all cases of lung cancer during the period of study was 61 out of 403 (15.1 %). the rate of lung cancer in none smokers, in the current study, was 39.3 %. Old age individuals were the main age group affected and there was female predilection. There was also no significant difference in the distribution of patients and control subjects according to residency, occupation, level of education marital status and socioeconomic status (P > 0.05). In the current study, the prevalence rate of passive smoking in patients was significantly higher than that in control subjects, 70.8 % versus 40 %. Moreover, the number of household smokers was significantly higher inpatients group than in control group. Conclusion: Lung cancer in non smokers appears to be significantly correlated with passive smoking in addition to increase incidence in old age and women.
25 The effect of Essential oils on Mutlit-drugs resistant Pseudomonas aeruginosa (MDRPA) , Lubna Abdulazeem, Hadeel Alaa Alrubaei, Farah Tareq Al-Alaq, Yasir H Al-Mawlah, Ali Shakir Obed
One hundred twenty five isolate of Pseudomonas aeruginosa isolated from different clinical specimens of patient’s in Al-Hilla, General teaching hospital, in Babylon, Iraq. These samples are fully characterized & identified by standard bacteriological procedures, used biochemical test & VITEK2 system compact. P. aeruginosa is intrinsically resistant to variety of antimicrobials & can develop resistance during anti-pseudomonal chemotherapy both of which compromise treatment of infections caused by this organism. Antimicrobial susceptibility pattern was carried out by the Kirby- Bauer disk diffusion method as per CLSI guidelines. Susceptibility test for anti-pseudomonas drugs show multi-resistant to most drugs except colistin and there was resistance to Ampcillin (80%) Carbenicillin (98%) Chloramphenicol (99%) Rifampin (97%), Imipenem (35%) mcropenem(36%), Tetracycline(89%) and colistin(0%). Traditional medicines are trusted as a source of potential antimicrobial agent thus essential oil from source like medical plants, herbs are used. The purpose of this study was to evaluate the antibacterial properties of essential oil such as: Syzygium aromaticcum (clove oil), Allium sativum (Garlic oil), Origanum majorana (majora oil), and Cinnamonumn zeylanicum (cinnamon oil). These oils expressed antimicrobial activity against clinical isolated of P. aeruginosa. Cinnamon oil had strong inhibitory against MDR strains are compared with other oil
26 Using PCR & RFLP techniques to detect the relationship between mutations (C677T & A1298C) of MTHFR gene and homocysteine level of heart patients, Mohammad Mutlag Salih, Akeel Hussein Ali
The aim of this study was to assess the level of homocysteine in patients with myocardial infarction compared to healthy patients. The genotype of the MTHFR gene, which causes high homocysteine level in myocardial infarction, was collected from 25 healthy individuals (control group) and (50) patients with myocardial infarction who were admitted to the intensive care unit. Those who suffer from myocardial infarction due to elevated homocysteine levels. Genomic DNA was extracted from the blood for study samples to determine the genetic mutation using specific primers and using The PCR-RFLP techniques, the study showed that the levels of homocysteine in blood plasma in people with myocardial infarction were significantly higher compared to control group. The study showed the relationship between the genetic diversity of the A1298C, C677T, and MTHFR genes. The results showed that the ratio The C677T allele did not show a significant difference between patients and healthy patients while A1298C allele percentage showed a statistically significant difference between the patients and the healthy at the probability level (P≤0.05) in the studied society. The genes of the enzyme A1298C, C677T, MTHFR showed additional mutations in patients with myocardial infarction Patients (Saladin community) compared with control group.
27 Genetic polymorphisms of ATP6 gene in vitiligo patients , Israa Harjan  Mohsen, Hadell Alaa Al-Rrubaei, Suad Ghali Kadhim Alahmed, Mona Najah Al Terehi
ATP6 gene represent mitochondrial gene that play important role in oxidative phosphorylation process which is important for ATP production and the occurrence of mutations in ATP6 gene and associated genes may disrupt mitochondrial oxidative phosphorylation and this correlated with a different diseases.This study attempt to detect the association between polymorphisms in ATP6 gene and vitiligo. The results showed the presence of deletion in ATP6 gene was (16%) of patients group while in control group was (5%) while the normal pattern was ( 84%) in patients and (95%) in control group.The deletion in ATP6 gene may associate with the loss of melanin from melanocyte in vitiligo patients and this needs more investigation.
28 Improve MRI in the diagnosis of neurological diseases , Marwa H Abbas, Zena N Al-Qudsi, Layth M Karim
MRI contrast agents are molecules to improve the quality of diagnoses by medical imaging magnetic resonance. The currently marketed agents are characterized by pharmacokinetics at so-called interstitial distribution, that is, after the injection intravascularly; the agent splits into every extracellular space. These agents allow detection of tumors brain, breast osteo-articular pathologies, and allow perform the imaging of the vessels called procedure angiography (Figure 1).
29 A study on measuring patients' satisfaction using SERVQUAL Model in Melaka private hospital , Lizwa Rashis, Amira Azman, Sue Pei Shin, Samer Ali Shami
There are many kinds of sectors or industries in a country. However, healthcare industry is important as it is the driver for the financial development and economic growth. Therefore, the quality of services delivered by the healthcare industry particularly in the private hospital should be excellent in order for the patients to be happy with the service quality , which can help in building customer loyalty and eventually provide profitability to the private hospital. The aim of this research is to describe the significant influences in the quality of services that affect patients’ satisfaction using SERVQUAL Model in the private hospital. The outcomes of this research paper would be able to represent whether the patients in the private hospital are more concerned with quality of services. In this study, five independent variables which are tangibility, empathy, assurance, reliability and responsiveness of the quality of services in measuring satisfaction of the patients in the private hospital. Next, secondary data such as literature review was also used in this paper. The proposed research framework is developed after understanding and interpreting the information from the literature review from different perspectives of researchers. Besides, primary data of this research is collected from 150 questionnaires among patients in a private hospital using probability sampling techniques which is the cluster sampling technique through drop and collect questionnaires distribution. The findings of this research should contribute to organization and government to identify the importance of private hospital of Malaysia for economic growth and improvement of the healthcare industry itself.
30 Factors affecting Students Continue Intention to Use MOOCs, Benefits and Drawbacks: A review Paper from the UAE Context, Salem Abdullah Aldahmani, Samer Ali Al-Shami, Muhamad AfzamimanAripin
In the twenty-first century, universities have misplaced their monopoly of the production and transmission of knowledge. They face the assignment of adapting to the needs of society, which can be summarized in three key aspects: economy, science and school development. The use of information communication technology has become the main concern for almost all educational institutions due to its cost efficiency that makes it affordable for all students regardless their economic condition and time effectiveness regardless the physical location. These magnificent advantages motivated not only educational institutions but also the ministries of education in most of the countries to adopt this technology as a key driver for socioeconomic development and illiteracy eradication. The UAE has its own agenda in adopting MOOCs in education system and at the heart of them is improve the quality of education. Despite, these advantages, many challenges still surround this vital technology. Thus, this research is a review paper on the introduction of MOOCs, its advantages and challenges and the trend for future research from the UAE perspective.
31 Therapeutic drug monitoring of cytotoxic drugs , Qutaiba Ahmad Al Khames Aga, Yazan A Bataineh, HalaMousa Sbaih
The majority of anticancer drugs are recognized with narrow therapeutic index, the area under the plasma levels versus time curve (AUC) is the common pharmacokinetic (PK) parameter which utilizes specifically for cytotoxic drugs. TDM approach in these drugs has never been completely applied due to different reasons, for example, the use of combination chemotherapies for different malignant tumors, and the behavior of intracellularcompounds; it’s possible to eliminate these limitations by using specific concentrations of cytotoxic drugs and measure AUC after certain conditions. In this review article, we discussed the common TDM parameters, methods of analysis, and some of drug interactions for a group of cytotoxic drugs.
32 The Advantages and Challenges of mobile health service quality system in UAE and future research , Halima Alblooshi, Samer Ali Al-Shami, Safiah Sidek
Innovations in mobile technology (mhealth) have brought remote healthcare management to the forefront of advanced medical care. The ability to record the cardiac activity of a patient at home has been available for some time. Due to the role of mhealth in improving the quality of health services in a timeresponsiveness and cost efficiency, the UAE health authority emphasized to the importance of mhealth and many hospitals introduced it with aiming to improve the quality and gain a competitive advantage. Even though the UAE is a leading country in health management, several challenges still surround their operation and impede the development of mhealth. Therefore, this paper aims to identify the potential advantages and the challenges that encounter mhealth development in the context of UAE. The finding of this research emphasized that the UAE health industry has the potential to develop their health quality through the development of the quality system of mhealth based on customers’ perspectives that meets customers’ satisfaction and continue intention to use mhealth
33 Genotyping of Glutathione-S-Transferase M and T in vitiligo patients , Noora M Hameed, Hadeel Alaa Al-Rrubaei, Zeena H Alwan
Vitiligo has become one of the important diseases which cause loss of skin pigments. Present study was carried out to investigate whether vitiligo is associated with GST genes polymorphisms in Hilla city. Blood DNA was extracted from patients and control then multiplex PCR was used to detect polymorphisms of GSTT and GSTM. The results of present study show that there were four genotyping in present study tow genes appeared together GSTM with GSTT, single gene GSTT only or GSTM and nor GSTT neither GSTM (null),The GSTT and GSTM appeared in 8 patients and 12 control at significant differences (od 3.9231, CI95% 1.3066 - 11.7793, P value 0.0148), GSTT was normal in 11 patients and 10 control at significant differences also (od 6.9697 CI 95% 1.5916 - 30.5199 P 0.010), GSTM was normal in 9 of patients while didn’t appeared as single gene in control (od 0.2180, CI 95% 0.0101 - 4.7164 P 0.331) both genes didn’t amplified for 14 patients and 3 control. The current study concluded that there was strong correlation between GSTT and GSTM polymorphisms with vitiligo disease.
34 Puemothorax: One year experience in tersm of dianosis, treatment and complication , Yaser Aamer Eisa Alhaibi
Background: The term ‘Pneumothorax’ denotes air in the pleural space, i.e., the space between the chest wall and the lung. Pneumothorax is generally classified as spontaneous (primary or secondary), which occurs without preceding trauma; traumatic, which occurs as a result of direct or indirect trauma and iatrogenic. Pneumothorax can be diagnosed by the history and physical examination in slender patients who have had acute onset of chest pain and dyspnea, and confirmed with a chest radiograph visualizing the visceral pleural line, however, the diagnosis of tension pneumothorax must be made clinically, because there is not enough time for imaging studies. Treatment options are: observation, needle aspiration, Tube Thoracostomy, Tube Thoracostomy with Instillation of a Sclerosing Agent, surgery. Method: Cross sectional study with analytic elements was performed by retrospective collection of data from 30 patients, at Alkadhmiya teaching Hospital, over the year of 2016 Objective: To know the most common cause of pneumothorax, what modality of treatment that is most commonly used in managing patients with pneumothorax, whether it is associated with complications or not, and the amount of time patients usually spend in hospital for follow up. Results: Trauma was the commonest cause of pneumothorax (67%).Usually patients who were admitted to the hospital didn’t have comorbidities (43%), however, (27%) of them were smokers, (30%) had tension pneumothorax.(87%) of the patients were treated by an underwater seal chest drainage system. Most of the patients (63%) stayed less than six days at hospitalAlmost all of the patients (93%) had no complications. Conclusion: Trauma was the most common cause for pneumothorax, affecting males more than females who are usually young adults.Patients most commonly treated by Chest Tube drainage,almost all of the patients didn’t have complications afterwards, and patients usually stay less than seven days at hospital for follow up.
35 Causes of Iron Deficiency anemia in patients sample attending the hematology outpatient clinic of Baghdad teaching hospital, Iraq, 2018 , Alaadin Sahham Naji, Luma Issa Hammoodi, Mustafa Ahmed Abdul-Lateef
Cross-sectional study was carried out on 104 patients (89 women and 15 men) in the Hematology outpatient clinic of Baghdad Teaching Hospital. The data was obtained from the subjects by an interview using a structured questionnaire with reviewing their medical records. Iron Deficiency Anemia was define as Hemoglobin level less than 13 g/dL in men, less than 12 g/dL in non-pregnant women or less than 11 g/dL in pregnant women, serum Ferritin less than 15 and MCV µg/L less than 78 fL Results: The mean of age of our study population was 36.2 ± 14.2 years old. Blood loss accounted for 69.3% of all cases, Malabsorption: 13.5%, Pregnancy: 11.5% and iron deficient Diet: 1.9%. In females, Gynecological related conditions accounted for 62.9% of the cases. In males, gastrointestinal blood loss accounted for 60% of the cases. There was significantly association between gender and gastrointestinal blood loss (P value = 0.001) and Malabsorption (P value = 0.015) as causes for Iron deficiency anemia. Conclusion: Blood loss was the leading cause of Iron Deficiency Anemia in both men and women, followed by Malabsorption. There is a significant association between gender with gastrointestinal blood loss and Malabsorption as causes of Iron deficiency anemia, both of them being more prevalent in males than in females. On the other hand, no association was observed between these causes with age categories. Gastrointestinal bleeding was the most common cause of Iron deficiency anemia in males, while in females the most common cause was menorrhagia.
36 Attitudes and Practices Associated with Blood Donation among Iraqi Donors Attending the Iraqi National Blood Bank, Baghdad, Iraq, Alaadin Sahham Naji, Luma Issa Hammoodi, Aiya Laith Kadhim, Dina Mohammed Abd al-Majeed, Rafal Mohammed Mandoo
The donation of blood by voluntary non-remunerated blood donors is defined as being very important for the safety and sustainability of national blood supplies. In Iraq voluntary donors are the only source of blood donation. Blood is the only oxygen transporter in the body and is crucial in saving lives. So far, there is no real substitute for human blood .Therefore, blood donation by humans will continue to be the major source for blood and blood components. Blood donation is truly ‘a gift of life’ that a healthy individual can give to others in their community, who are sick or injured Aim of the Study: Determine the attitude and practices towards voluntary blood donation, describe previous experiences and barriers to blood donation and what factors that play a role in the recruitment and willingness of donors to donate blood voluntarily Methodology: This is descriptive cross-sectional survey design (from 1st July 2017 to 1st October 2017). Data was collected by interview questionnaire from 500 blood donors. Subjects were selected because of their convenient accessibility and proximity to the researchers therefore a convenient sampling design was utilized. The interview questionnaire contained 21 questions divided into three sections to obtain data regarding general information. The information included religion, background education, occupation, economic status, whether he or she donated blood before, the motive behind donation, and attitude regarding blood donation. Results: The study was conducted on 500 participants’ attendee to Iraq National Center Blood Bank, 477(95.4%) were males and 23(4.6%) were females. The most frequent age groups of donor between 26-35 (42.4%) years old. The educational qualifications were primary school graduate (25.4%) and intermediate school graduate (24.6%), with only (19.2%) having a bachelor's degree. The majority stated that they are freelancers (49.4%) and (40.2%) were employees. Out of the 500 participants there was 407(81.4%) who stated that they had donated blood before, the percentage of those who had donated once before in their lifetime was (16.7%) and about (67.1%) had donated more than once The main reason for donation was the sense of responsibility in about 41.5%, while 23.6% was to help a relative\ or a friend, and some reported their reasons were because of a certain medical condition (22.4%), the remaining reported not having a specific reason (5.7%), religious\spiritual reasons (4.9%), and blood donation campaigns (2%). Of the 93 (18.6%) who had never donated blood before, (74.2%) reported that not having an opportunity to donate was their main reason, while 12.9% stated that they were not eligible to donate because of a certain medical condition. For attitudes of the donors towards voluntary blood donation; it shows that almost all the participants (99.2%) believed that voluntary blood donation is important and that it could probably save a life. The study revealed that almost half of the study subject (42.4%) did not have any information about the medical and social benefits of blood donation, but 58.6% claimed to have some information about that. Conclusion: This study revealed the domination of male gender as blood donors who came from the vicinity, and the positive attitude was derived from the Iraqi donor's altruism were the main factors that influenced the recruitment of voluntary blood donors
37 Video Qualitative Analysis of Soccer place-kick , Abdullah Hussein Al-Lami, Rashad Abbas Fadhil, Haider Ali Hadi
Biomechanical analysis of any sporting activity provides both quantitative and qualitative information about the event. This present work aims to examine the sequences of different body segments before, through and after side place-kick. Ten students taking advanced course at the Physical Education Department alerted University agreed to take part in this study.Qualitative investigation of the position of the non-kicking foot, degree of trunk lean back, the position of the kicking knee in relation to the ball and the pattern of the follow-through is presented, and showed two different patterns among players in positions of the body segments before, through and after the kick.
38 Impact of using training loads with biorhythm on the performance of kinetic series Conkday for karate players, Mohannad Nazar Kzar, Retha Abd Alhussein Hadi, Ameen Salih Ateya
Karate sport is considered one of the individual combat sports which has been the subject of scientific development, characterized by a variety of basic offensive methods between punching, hitting and kicking, which requires defensive and offensive performances with arms and legs strong and fast up to the skill time in the performance of less than one second and high intensity and repeated in a time In Kata, the performance of the skill and the constant and immobile muscular contractions continue in performance over the Kata period, which can range from 60 - 90 seconds depending on the type of kata and the school to which it belongs. The researcher considers the need to search for new methods and approaches to increase the effectiveness of the training programs, in addition to not increase the training loads, but also to make maximum use of the potential of the training case for the player and benefit from applied biological sciences in developing the level of training for the player and the reflection on the level of special achievement (Armstrong, 1998).Objective of the research: To know the effect of training loads according to the nature of the biorhythm on the level of performance of the kinetic series of Kata Conkday Karate. Objective of the research: To know the effect of training loads according to the nature of the biorhythm track on the level of performance of the kinetic series of Kata Conkday Karate. The research hypothesis: There are statistically significant differences between the values of pre- post measurements of the level of performance of the kinetic series, which is oriented according to biorhythm track.
39 The effect of use computer technology in the process of mental training in learning some skills of volleyball for juniors, Samir Yousif Mutib, Mazin Hadi Kzar, Ahmed Yousif Mutib, Ali Abdulkareem Dohan
There is a weakness and difficulty in forming a model image for the skill according to performance requirement for players during the imagination in the mental training process and this is reflected significantly on the player's performance during the match as well as weakness in performance of the basic skills of the juniors. So the researchers want to get solution for problem by activate the process of creating the mental image of the model of skill during the mental training of the players using the displays provided by the computer like normal-motion, slow-motion, (3D) photos and videos addition to the biomechanical analyses. The aim of study is know the effect of use computer technology in the development of some volleyball skills of players. The hypothesis of research is that there is a positive effect of use of computer technology in development of some volleyball skills of players. The researchers used the experimental method by design the equal groups. The sample included the juniors (14-16 years) in Hashemiea Club / Babylon city, their number is (24). The data was analyzed by using the statistical program SPSS. The researchers reached to number of conclusions were, the most important of which was the positive effect of computer features in activate the process of forming the mental image of the skill during the mental training, as well as having a positive effect on developing the performance of some basic skills players.
40 The Impact of Exercises in the Style of Competition on Some Hormones and Variables of the Immune System in the Blood for the Category of Badminton Players who are under 17 Years, Mohannad N K Al-Swidy, Maher AHH Al-Alwani
The aim of this research is to identify the impact of exercises in the style of competition on some hormones , like (TSH, T4, T3) and the variables in the immune system in the blood(i.e. the total number of white blood cells, neural cells, acid cells, basal cells, lymphocytes and single- core cells) for the flying feather category of players who are under 17 years.The researchers use the curriculum demo on a sample which consists of (6) players who represent the National Iraqi team of flying feather for the category of players under 17 years. In addition, they use the means and the tools for gathering information as: scientific sources syringes, pipes, a box for drawing, collecting and saving blood, electroscope to analyze the hormones. The researchers reach to numbers of conclusions and they are as follows. First, there is a negative impact of the effort exercises in the style of competition on some variables in the immune system in the blood (i.e. the total number of the white blood cells, neural cells, acid cells, basal cells, lymphocytes and single- core cells) of the badminton players. Furthermore, there is a positive impact of the exercises in the style of competition, on some blood hormones (T3, T4, TSH) for the badminton category of players under 17 years.The recommendations are: adopting the results of the research and make use of them in recognizing the variables in the immune system that take place before and after the exercises in the style of competition, as well as, adopting the results of the research and make use of them in knowing the functional indicators (hormones) before and after the exercises in the style of competition.
41 The Effectiveness of Teaching Using the Marzano Model for Learning Dimensions in Increasing Cognitive Achievement and Learning Some Basic Skills among Students in Tennis, Mazin Hadi Kzar, Dhafer Namous Khalaf
In order to verify the effectiveness of teaching using the Marzano model for the dimensions of learning in increasing cognitive achievement and learning some basic skills in tennis, the researchers used the experimental method on the research sample of (30 students of the third stage in the Department of Physical Education and Sports Sciences - Faculty of Al-Mustaqbal University College and after the implementation of the field procedures to search and get the findings. The two researchers reached the most important conclusions: The Marzano model of the dimensions of learning provides an integrated educational system as it is concerned with presenting concepts and ideas at the beginning of each lesson in the form of cognitive maps and conceptual schemes that illustrate the relationship between the skilled Basic question and the importance of interdependence between them. The researchers recommend the use of the Marzano model in teaching the other basic skills in tennis and other materials as well as conducting research and similar studies using modern teaching models, which proved effective in teaching the various sports materials and the different stages of the college.
42 The Effect of a Rehabilitation Program on the Development of Certain Physical Abilities of Volleyball Players with Outwards Bowed Legs (Genu Varum), Tahseen Abdul Kareem, Mazin Hadi Kzar, Samaher Salman Alwan
The different types of limb deformities, including outward bowed legs (genu varum), are mainly a result of osteoporosis due to an unrecovered vitamin D deficiency during childhood, environmental pollution, genetic disorders, improper medication use, congenetical malformations, certain cases of obesity, or Blount's disease, whose symptoms may not appear until adolescence. Since genu varum results in thin and brattle bones, certain rehabilitation programs are required to resolve the leg deformity. If such correction treatment is left out, the symptoms may develop into osteoarthritis and the breakdown of the joint cartilage, which makes it even more difficult to do any physical leg exercises. The rehabilitation process builds up muscular abilities of the thighs, knees, and lower legs which are essential to perform the rehabilitation activities by means of various supporting tools and light weights that reinforce to the correction of the volleyball players' leg deformity. This all lead to the main aim of the research, namely to develop a rehabilitation program to treat or, at least, lessen the curvature of the knees and to improve certain physical abilities at volleyball players who suffer from genu varum. The researchers adopted the experimental approach to solve the research's problem. The research sample is represented by (8) volleyball players who suffer from outward bowed knees. The rehabilitation program used by the researchers over a period of two months, under the supervision of medical specialists, included various rehabilitation exercises, supporting tools, and several medical tests. The researchers concluded the following: The rehabilitation program set up by the researchers affected the volleyball players positively as it corrected the leg's curvature more to a certain extent. And the rehabilitation program has a positive influence on the development of certain physical abilities that contributed to the correction of the volleyball players' leg deformity.
43 The speed of motor response and its relationship to successful attack for players (Iraq, UAE, and Tunisia) fencing, Mazin Hadi Kzar, Dhafer Namoo Al-Taie
It is obvious to adopt the successful attack of the fencing player to the speed of his response to changing situations which occur in the game, and to identify the type of relationship between the speed of the motor response and the successful attack of the young fencing players and participants in the Arab Championship held in Cairo in 2018. The researcher used the descriptive approach in the method of associative relations, after applying the test to the research sample of (12) new players representing teams (Iraq, UAE and Tunisia), and got their results players in the championship of the summary results which were statistically treated, and the researcher reached the most important concluded, The speed of the motor response is critical to the successful attack by the young fencing players through the moral relationship between them. The researcher recommends the need to pay attention to training the speed of the motor response according to the scientific bases and included in the training programs for trainers. As an important ability for the fencing player. And other research on the relationship of physical, motor, skill and planning capabilities among them for Arab players and all levels and compare them to the world champions.
44 The physical effort influence according to the level of mct1 gene in the recovery of lactic acid in basketball players, Mohammed H Shaalan, Rashad Abbas Fadhil, Haider Ali Hadi
The study aimed to discover the genetic different of basketball players Al-Hilla Sports Club for the sports season (2017-2018) after practice a physical effort by the lactic test(MLSS) in the speed of recovery and of lactic acid. The research was limited the sample (12) players from Al-Hilla Sports Club. The first stage was the knowledge of the genetic diversity of the players. The first level of the gene (5 players), the middle level (3 players), the minimum level of the gene (4 players) and the second stage The experiment was to measure the variable (lactic acid) for all levels of the gene and to detect the players who had a rapid elimination of the results of lactic acid. The experiment lasted for 2 months. The most important conclusions were the high level of the mct1 gene in the rapid elimination of lactic acid in the working muscles.
45 The effect of hypermedia program in generalization of motor program of shooting skill in basketball for students, Mazin Hadi Kzar, Samir Yousif Mutib, Maher Abdel-Hamza, Haider Na'eem Alabbosi
One of the reasons that led the researchers to do this research is that there is weakness in students’ performance of shooting skill in different types according to playing requirements, because of the learning process that is used only to teach students in one case and in constant conditions and this indicates a case of weakness in learning in general and in generalization of motor program of shooting in particular, so, to treat this weakness, the researchers suggested the use of good learning method to overcome those problems, the one of important means is hypermedia. The first aim was the preparation of hypermedia programs for shooting skill of basketball, while the second aim is to know the effect of hypermedia program in generalization of motor program of shooting skill. The hypotheses of the research were that there is a significant effect of hypermedia on generalization of motor program for shooting skill. The research population was identified by the students of the second stage in the College of Physical Education and Sport Sciences, Wasit University for the academic year 2014 – 2015. The research sample was selected by a simple randomly method which consisted of (30) students divided into two groups (experimental group and control group by 15 students per group).The researchers used many standardized tests for shooting skill of basketball and they performed the pre-tests and after that applied the learning curriculum, which followed by a post- tests to get on the research data that has been analyzed statistically using statistical program (SPSS).It reached a set of conclusions. The most important was the use of hypermedia program helped in develop and generalize the motor program for shooting skill of basketball.
46 Effect of some exercises in development of turning phase and the performance in 100 m freestyle of youth swimmers aged15-16 years, Mazin Hadi Kzar, Hammoudi Mahmoud Ismail
This research was to examine the effects of some exercises to improve the turning phase in 100 m free-style performance. The sample of the research was 16 swimmers selected randomly and assigned into two groups, the first as experimental, which executed the exercise arranged by the researcher, the other was control group, which used the usual exercise selected by their trainer. Both groups were tested pre & post training in 100m free-style swim, under same conditions. After 12 weeks the researcher found that both groups had an improvement in 100m free-style swim performance, but with different ratio. Yet the group which used the researcher exercises had a significant value than the other. The researcher recommends using the exercises concerning turning phase to improve the performance in 100m free-style.
47 Comparing the effect of eggshell membrane, olive oil, or castor oil as a skin anti-aging agent , Basima Jasim Mohammed, Shaimaa Abbas Sabeeh, Ali Habeeb Jaber, Orooba Meteaba Faja
Background and aims: Skin aging is a life-annoying problem especially for women that needs to act so that appearance of signs of aging can be delayed as long as possible. Here, the current work was intended to discover the effects of eggshell membrane (EM), olive oil (OO), or castor oil (CO) as a skin anti-aging agent (SAAA). Materials and methods: The thirty-day study included the use of randomly-divided four groups of 20 rabbits, T1 (oral-administered with freshly egg-extracted EM 2gm/day), T2 (oral-administered with OO 5ml/day), T3 (oral-administered with CO 5ml/day), and T4 (nothing supplied). After the experiment was done, the parameters measured for detecting the effectiveness of the substances were component of skin amino acid (glycine, alanine, proline, and hydroxyproline). In addition, histopathological examination of the rabbit skin was conducted for 2 time points (one week and six weeks after starting of the experiment). Results: The results of T1 showed significant (p˂0.01) higher percentages of components of amino acid in the rabbit skin than those from the other groups. Moreover, the histopathological examination revealed that T1 demonstrated the strongest proliferation of epidermal cells in the wrinkle of rabbit skin for the two time points when compared to the other groups. Conclusion: The current study demonstrated the promising effects of the eggshell membrane as a skin anti-aging agent.
48 Treating mice using improved pomegranate peel techniques in the treatment of parasite , Marwab Thamer Abbas Al Saadi, Noor Thamer Abbas Al Asadi, Zina Tahsin Ali
Pomrgranate (Punica granatum) was evaluated for treating infected mice. The current study included the test of the efficiency of the water and alcohol extract of pomegranate peel. Experimentally infected mice were stimulated with the water and alcohol extracts of the pomegranate peel with a concentration of 15.657 giardia lamblia.Amalgam / mouse and 21,893 mg / rat, respectively, and one dose daily. The therapeutic efficacy of the extracts studied was compared with metronidazole which was given at a dose of 0.393 mg / rat / day. The results of the present study showed the efficacy of both metronidazole and pomegranate peel extracts studied in the treatment of parasitic infection. All treatments were fully effective and effective in treatment (100%), but varied in the time required for complete treatment.
49 A new record of three bdelloids from Babylon province-Iraq , Sara Khalid, Aljaryan Adi Jassim, Abd Al-Rezzaq
There are a few classification study of Rotifer in Iraq which classified some bdelloids in fresh water, but this is the first study of bdelloids collected from soil and sediment habitats at five different site in Babylon province – Iraq. This study add three Bdelloids species (1Philodina22acuticornis,4Murray,51902 ,6Philodina 7plena 8(9Bryce0, 118942) and3Philodina roseola Ehrenberg, 41832) to Iraqi fauna with scanning electron microscopy study for its trophi.
50 Role of antibiotics in perianal abscess , Suhad Kahdum Ali
Purpose: In this study, type of organism was discovered in the surrounding anal abscesses and its sensitivity towards antibiotics was also evaluated. Sixteen diseases were reported, as was the study of skin rupture or thrombocytopenia during this study. Using povidone iodine and alcohol the skin was purified by using a needle syringe 5 cc, the identified parasite was examined and sent for antibiotic susceptibility tests. Also common aerobic and anaerobic culture test was utilized Results: The rates of growth of anaerobic and aerobic bacteria recorded (100%, 87%) were severe. Aerobic bacteria, Escherichia (13/16), Eubacteria spp. (4/16), intestinal pulmonary bacteria (4/16), Citrobacter freundii (2/16), enteric intestinal Enterogens serogroup D (1/16), and Staphylococcus aureus (1/16) were identified. The antibiotic sensitivity rate was determined for E.Coli to be, 85.6%, 68.2%, and 31.8% Sivazo-Lin to Amoxicillin-Clavolamic Acid, Penicillin, Antibiotics. The rate of resistance of amikacin was 33%, E. coli resistance to gentamicin was 30%, and antibiotic sensitivity rates for anaerobic bacteria were 100%, 79.6%, 72.4% and 58.1% for the ampicillin-sulbactam, proteosol, clindamycin, and penicillin, respectively. The rate of fistula development in the follow-up of the disease for 12 months was 28% and 36% in patients with acute airway infections. Conclusion: The main alternative to oral antibiotics has been demonstrated in this study to treat the symptoms of fistula. The drug should be antiviral plus an increase in the proportion of cefazolin. In the case of aminoglycosides, it is used in severe cases. Amikacin is suggested to be administered because our findings show increase levels resistance of gentamicin
51 Influences of sound loudness on the registration of vertical dimensions , Rafah Habib Abdul Amir
Aim of study: 1- To evaluate the effect of sound loudness on the vertical dimensions, and compare it with the swallowing. 2- To evaluate the effect of sound loudnesson the closest speaking space. Materials and methods: 160 Iraqi students consisting of 50% males and 50% females aged ranging from 18-21 years with certain criteria were included in this study; the rest vertical dimension was measured by using the "M" sound method in twointensities of sounds (whispering and normal speech). The closest speaking space was measured by using the S sound in two intensities (normal and whispering) in the same manner as the measurement of the M sound. Results: a. Swallowing method give free-way space varied between 1—4 mm, majority of sample is of 2-3 mm. b. Phonetic method (whispering) give free-way space varies between 1-6 mm (male) and 1-8 mm (female), majority of sample was of 2-4 mm. c. Phonetic method (normal speaking)give free-way space varies between 1-7 mm (male) and 1-8 mm (female) majority of the sample is of 2-4 mm. Conclusions: 1-it has been found that phonetic method even whispering give rest vertical dimension greater than swallowing method, and this comes in contradiction to the assumption of joint B.
52 Evaluation of male and female rugae area of Iraqi population using 3D EXO-CAm scanning EXO-CAD designing software , Eman Alayah, Sara Mualla
Aim of study: To evaluate rugae area pattern, average length, depth of different samples of different Iraqi population and compare the parameters between males and females. Materials and methods: 60 students at Alturath University selected for this study (30 males and 30 females), age ranged between 18-25 years with definite criteria as (no missing teeth, no crowding, and no oral defects), impressions were taken with alginate from which stone models were fabricated. Each cast was scanned with EXO CAD scanner, 3D image of the casts investigated using the EXOCAD measurement tools software. The length of the rugae area, the patterns of rugae and symmetry of the primary rugae between the right and left wings of palate were checked. Results: two tailed T test used for statistical analysis. The two tested groups showed highly significant difference from each other both regarding the length and depth of rugae area. The means of each type of rugae area was measured and compared. V. CONCLUSION: Within the limitation of this study it can be concluded that 1. There is a significant difference between Iraqi males and females regarding the rugae area length and depth with females having the longer and the deeper rugae area. 2. Curved form was the predominant in females and the straight form counted the highest in number in males. 3. Both groups possessed more primary rugae area than secondary or fragmentary. 4- Each individual had different rugae area pattern from the others and can be used in human identification and sex differentiation. Yet, further studies of cross-sectional nature are recommended to discern the methodology of using the palatal rugae area in forensic gender recognition.
53 What is the role of governance in the health national innovation system? A Logic model approach , Hesam Seyedin, Majid Heydari, Azadeh Ahmadzadeh Ghasab,  Omdi Khosravizadeh
Background: Government as the most important components of innovation system has an important role in the efficiency of Health National Innovation System (HNIS). This study was conducted to identify the role of the government in the Iran's HNIS. Materials and Methods: The study was conducted by Focus Group Discussion (FGD). Results were arranged by logic model approach in categories of inputs, process, outputs and outcomes. Results: HNIS inputs that government has role included: availability of credits and financial resources, policies and programs, innovative public and private organizations, industries, innovative human capital and supply of technical knowledge. In HNIS activities: resource allocation, support of innovation, human capital empowerment, interaction and communication with industries and international organizations, managing of developmental programs, monitoring and control of innovation system. In HNIS outputs: supportive programs, guidelines, regulation and memorandum of interagency cooperation, innovation policy and innovative education programs. In early outcome: efficiently resource allocation policies and guidelines, increasing inter-sectorial cooperation, increasing motivation of organizations to conclude agreements with international institutions and improving organizations' attitudes to innovation projects as a source of income. In intermediate outcomes: implementation of innovative policies, constant monitoring of the implementation of innovative policies, optimum use of funds, strengthening innovation supporter institutions, sustainable collaboration with industry and commercialization of new ideas. In late outcome: improve public health, economic boom, create wealth by using new ideas, increase satisfaction, transform ideas into products and expand the culture of innovation in society and organizations. Conclusions: Government has various roles in Iran's HNIS. By identifying the role of government at various levels of HNIS can measure its performance. Better understanding of the government performance will lead to formulation of better policies and programs
54 The effect of long-term storage on mechanical and structural properties of glass and glass-ceramics  mixed with radioactive waste , Al-Dafaee, Thuraya Amir, Al-Mashhadani , Asia H
In an attempt to ascertain a suitable method for immobilizing long term disposal of radioactive solid waste (Strontium oxideofmolecular weight 5%) a verification method was adopted in two types of borosilicate glass (c-type) ( glass and glass-ceramics). To investigate the effect of Gamma-ray on mechanical properties of glass material and possible leaching, a Scanning Electronic Microscope (SEM) technique was adopted. The collected data showed that storage of radioactive nuclear waste in glass-ceramics could be safe for long time storing.
55 Role of breast feeding in protection against myocardial infarction in Iraqi women , Israa F Jaafar, Safa Muntadher Fawzi
Abstract Background: Heart disease remains the leading cause of death for women in the world. Multiple lifestyle factors contribute to heart disease risk. In addition, hypertension, high cholesterol and diabetes increase the risk for myocardial infarction. Lactation may alter maternal glucose and lipid homeostasis and affect blood pressure regulation. Breastfeeding women have lower triglyceride and higher HDL cholesterol levels. Human studies indicate that lactation reduces blood pressure and heart disease. Objective: To explore the protective effect of lactation in cumulative number of years that women breast feed their babies on subsequent risk of obesity, hypertension, hyperlipidemia and cardiovascular disease among sample of Iraqi women. Subjects and methods: The Study was carried out in Al-Numan Teaching Hospital from April 2017 till February 2019. The study included 1200 women. 700 women fulfilled the inclusion criteria (Married with 1 child and more, lactating, have cardiac disease, non smoker, non diabetic). The rest 500 women were non-lactating with same criteria. Results: The study involved 1200 Iraqi women; their age was (44-67y). 700 0f them were lactating who have live births from (1-12) with mean of (5.171±2.425), the duration of lactation ranged from (3 -24 years) with mean of (8.557±4.405 year).The rest 500 were non-lactating. The body mass index (BMI) of both groups showed significant difference P≤ 0.0001. There was significant differences between the two groups regarding lipid profile including (S. cholesterol, TG, HDL and LDL) P≤ 0.0001 ,as well as atherogenic index of plasma(AIP) for lactating and non-lactating women the P value were less than 0.001. There was negative correlation between duration of lactation and BMI,AIP and myocardial infarction. Conclusion: Breast feeding protects the lactating mother from risk of CVD later in life by reducing BMI, AIP. The more duration of lactation the more protection given to mothers against CVD
56 Histological study of skeletal muscle regeneration stain by hematoxylin and eosin , Taghreed Abdulrool Ali, Aseel Kamil Hissein, May Fadhil Al-Habib
Skeletal muscles are the largest cells in the human body, the main function of skeletal muscles are to facilitate locomotion. Regeneration is process that rely on skeletal basement membrane, satellite cell (endogenous stem cells of skeletal muscles), and extra cellular matrix actuation.Sample of 40 mature male rabbits were used in operative week ). The Extensor digitorum longus muscle was set as a model for skeletal muscle injury and examined after 3&6 weeks initiation of injury. The muscles tissue were prepared and examined histologically using H&E. Morphological changes during the degeneration and regeneration was assessed thoroughly. The degeneration and regeneration were overlapping both in time and morphological cellular changes. Mononuclear cells infiltration with debris removal was the key step to eliminate degenerative faction and start of regenerative process. Early myoblast appearance and new myotube formation occurred during the 3rd week. By the end of the 6th week post operatively the muscle histological maturation and muscle fascicles .Not all injured muscle ended in complete recovery the muscle that showed abnormal infiltration of inflammatory cells, at early stage and ended with fibrosis. It was concluded that the process of regeneration is a dynamic type where degeneration and regeneration superimposed each other.
57 Studying the heavy metals of the Dijla river and Al Karkh water project , Wrood Kareem Abood, Liwaa Abed Shihab
The current study was conducted on the Karkh Water Project on the Dijla River in Baghdad Governorate. The station was selected as a project to filter water and feed Karkh water. The study included measuring the concentrations of some heavy metals in the waters of the Dijla river during the period from October 2018 to March 2019. Five heavy metals (cadmium, lead, chromium, nickel and mercury) were taken by three models of river water, The results showed that the concentration of heavy metals (nickel, chromium and mercury) was within the permissible limits, and nickel concentrations were few and were within the limits of Iraqi standards. The results of the study showed high concentrations of cadmium and lead, which were above the permissible limits of the standard Iraqi standard. If lead concentrations were ranged (0.005 – 0.15) mg/L, which was high compared to the allowed limits, the concentration cadmium were ranged (0.009 – 0.034) mg/L the is more than the permissible limits in all stages of drinking water. The values obtained (pH)were within a small range This is the normal rate of drinking water (7.2 -8)(pH).
58 Inhibition gene expression MexAB-OprM and MexYY efflux pumps of Pseudomonas aeruginosa (XDR) by novel inhibitor Levofloxacin, silver nanoparticles and Beta rays , Nebras Rada Mohammed
This study was achieved in order to determine the inhibition of gene expression of MexAB-OprM and MexXY efflux pumps by using different novel inhibitors including: Levofloxacin, AgNPs and Beta rays emitted by Tl208 and Sr90 on P.aeruginosa (XDR).Efflux pumps are responsible for pumps various kinds of antibiotics including quinolones (fluoroquinolones), Aminoglycosides, BLactam and Polymyxin for Pseudomonas aeruginosa(XDR). Levofloxacin utilized as novel inhibitor to MexXY and MexAB-OprM efflux pumps of P.aeruginosa (XDR) and the percentage of killing was determined. Detection of gene expression by using qRT-PCR, did not improved gene expression of MexXY and MexAB-OprM when Levofloxacin 50 mg/ml was added when studying the gene expression of mexX and mexB encoded to MexXY and MexAB-OprM efflux pumps. Silver nanoparticles(AgNPs) used as new inhibitor of MexXY and MexAB-OprM efflux pumps of P.aeruginosa(XDR), the percentage of killing calculated , the survey of gene expression by utilizing qRT-PCR was determined. The results did not show gene expression of the MexXY and MexABOprM when Silver nanoparticles100 mg/ml was added to study mexX, mexB and compared with control (without treating). Beta rays utilized as new inhibitors emitted ofTl208 radio source with activity 1 𝛍ci, dose 12.93*10-10 KGy for 3 hr.; also exposure to Sr90radiosource with activity 1 𝛍ci, dose 1.937*10-10 KGy for 3 hr. and exposure to Sr90radiosource with activity 9 𝛍ci, dose 6.3*10-10 KGy for 3 hr. The results exposition Beta radiation P.aeruginosa (XDR) showed the number of colonies less than the control with rise in the percentage of killing of P.aeruginosa(XDR), also it found the that morphology of the colonies changed compared with the original(control). Detection gene expression of P. aeruginosa (XDR) prior exposition to Levofloxacin, AgNPs, Beta rays and after exposition to Levofloxacin, AgNPs, Beta rays was achieved by qRT-PCR technique, RNA extraction of P. aeruginosa (XDR),synthesis of cDNA ,calculate gene expression according to Livak equation to detect gene expression of MexXY and MexAB-OprM efflux pumps with study of mexB and mexX gene. The results exhibit gene expression prior exposition to Levofloxacin, AgNPs and Beta rays were highly in MexXY and MexAB-OprM efflux pumps of P. aeruginosa (XDR) but fewer after exposition to Levofloxacin, AgNPs and Beta rays. The Levofloxacin, AgNPs, Beta irradiation were had competence in killing P.aeruginosa and qualification as novel inhibitors to inhibit gene expression of MexXY and MexAB-OprM efflux pumps P. aeruginosa (XDR).
59 Refractive surgery of myopia by LASIK, Z-LASIK, PRK, PHACO, and I.C.L. , Nebras Rada Mohammed, Aber Abdul Amir Mohamad
Refractive Surgery of Myopia performed by LASIK , Z-LASIK , PRK , Phaco and I.C.L. on 52 patients including 20 patients for LASIK surgery, also 20 patients for Z-LASIK, 8 patients for PRK, 3 patients for PHACO surgery and 1 patients for I.C.L., this type of surgery is used for vision correction because it is quick and painless. The patient’s vision was examined before and after the surgery by using Snellen chart to determined visual acuity; the corneal thickness, shape of cornea were determined by Topography; the refractive error was measured before and after surgery by using Auto refractor meter, also determine gender, age and degree of glasses for each patient. The refractive surgery of myopia was performed in order to discard the use of glasses. Patients after the 18 years were eligible for surgery using LASIK, Z-LASIK, PRK except PHACO and I.C.L techniques. The processes of PRK, Z-LASIK works with little thickness of cornea of 480 microns. High degrees of short sightedness were corrected by PHACO surgery due to the thickness of the cornea which could not tolerate scrape to perform the surgery for all grades. I.C.L. can be used better on young patients because after surgery, there is no need to glasses compared with LASIK,PHACO and Z-LASIK surgery may require glasses after surgery , this surgery undesirable for young. Surgery using PRK technique until 4 degrees of short sight safely while works LASIK surgery with more than 4- degrees occurs with LASIK surgery. Z-LASIK works only with high degree of myopia with a little cornea thickness of 480 microns, either with very low degrees of myopia with a cornea thickness of 500 microns or more works Z-LASIK and LASIK both.
60 Improving light-harvesting of 17 9-dye by using gold nanoparticles , Ghufran S Jaber
Currently dye-sensitized solar cells (DSSC) provide technical and economical alternative guaranty to pnjunction solar cell devices. Contrary to the general methods, where the semi conductors suppose two targets of light absorption and charge-carrier moving, the two tasks are split up in this region. Light is soak up by a dye-sensitizer, which is installed on overall the surface of the semiconductor. In the present work, gold AuNPs was elaborated by pulsed laser ablation in liquid LP-PLA at energy-750 mJand no. of pulses=90 pulse, then they were added to N719 dye to form new blend consist of gold nanoparticles and N719-dye. The distribution size of gold nanoparticles clarifies circular shape with particle size ranging around 60 nm. The different shapes of particles lead to different peaks of the surface plasma resonance (SPR). Nanoparticles have remarkable advantages in increasing the absorption of dye due to their tiny size and homologous with the medium. The dye absorbs the atoms of incident light (sun-light and surrounding of ambient light), using its energy to transfer electrons, can work similar to chlorophyll in photosynthesis. The UV-Vis spectrophotometer showed rang of wave length high absorption for N719 - AuNPs compared to N719 dye alone, supporting the role of gold nanoparticles in improving the dye absorbance.
61 The biological effects of leptin as topical treatment for traumatic oral ulcer in rats , Sabreen S Abed, Nada M Al-Ghaban, Natheir A Ibrahim
Back ground: The painful ulcer to the oral mucosa caused by trauma or diseases, represents the most chief complain of the patients attended to the dentistry clinic. The present study was undertaken to perform histologic and histomorphometric analysis of the biological effects of the topical application of leptin on induced traumatic oral mucosal ulcer healing. Materials and methods: In current study, forty eight male Albino rats weighing 200.45 - 270.53g, and age between 2-3 months, were subjected to traumatic ulcer with 8 mm diameter by surgical blade no.15 on the right side of the buccal mucosa. The animals were divided randomly into two groups; the ulcers of the control group treated daily with 10μl of sterilized distil water, while the study group ulcers treated daily with 10μl of 1 μg/ml of recombinant leptin. The rats were sacrificed at 3,7,10 days. The specimen were taken and prepared for histological examination, and histomorphometrical assessment of blood vessels account and re-epithelization thickness in the ulcer area for both control and study groups. Results: The histological and histomrphometrical result using leptin as a topical treatment showed reduced the inflammatory cells infiltration, accelerated the re-epithelization of wound surface, and enhanced angiogenesis in the ulcer area, resulting with fast tissue healing. Conclusion: Using leptin as a topical application in the present study for treatment an abrasion-induced ulcer, revealed that leptin can reduced the ulcer diameter and accelerated the healing process in a short time.
62 The effect of balance training on postural sensory organization against external disturbances in athletes with chronic ankle instability , Hadi Miri, Seyed S. Shojaedin, Amin Hossein Barati, Maliheh Hadadnezhad, Shahram Ahajan, Samaneh Soltani Najipoor
Introduction: Damage to the ankle-sensory-control system and disturbance in the integrity of sensory information and awareness of the sense of movement is an important factor in reducing the balance and is the result of re-injury and strengthening the effective factors in sensory organization and balancing and postural control. As an essential strategy in the treatment of balance and postural problems as well as athletes' injuries.Objective: To evaluate the effect of balance training on postural sensory organization against external disturbances in athletes with chronic ankle instability. Materials and Methods: In this semi-experimental study, 30 male athletes with chronic ankle instability were randomly divided into two groups of exercise (n = 15) and control (n = 15). The measurements were pre-tested and the test was carried out and the experimental group training program was six weeks of balance training against disturbances. To evaluate postural sensory organization, a dynamic computerized post choreographysystem was used. Which the state of the senses in 6 states that are in the first state (the presence of all three senses of visual, auditoryand proprioceptive), second (visual outage), third (auditory manipulation), fourth (manipulation of proprioceptive), fifth (visual elimination, proprioceptive manipulation) and sixth (manipulation of auditory sense and proprioceptive). Data analysis was performed using covariance analysis and all statistical data was performed by SPSS software version 22 at the significance level of α ≤ 0.5 .Results: The findings of this study showed that people in the first state based on the score of stability, sensory organization and better postural control than the other 5 had a better status. The score of sustainability in the sixth state, which affects every 3 sense, is less than other state. There was no significant difference between sustainability score in the second position (visual loss) and the presence of two senses,auditoryand proprioceptive (with the third condition), the auditory impairment and the presence of two senses of visual and proprioceptive (these results indicated that individuals were not able to balance in the second position visual and in the third position, they are not dependent on auditory, but between the second condition with the fourth condition(proprioceptive manipulation) fifth, (the removal of visual) and sixth(auditoryand proprioceptive impairment) the difference was statistically significant, and the subjects in the second position were significantly different based on the sensory organizing stability, balance, and postural control better than the fourth to Sixth, the mean sensory organizing in the experimental group after six weeks of training in all six conditions was higher than the control group. Conclusion: The results showed that athletes with chronic ankle instability in the training group had a better sense of organization in the group compared to the control group; external disturbances had effect on training group, because the balance training improved proprioceptive activity and sending information and caused neuromuscular coordination. In addition, the improvement in the integrity of the sensory inputs may change the pattern of motor control and speed up the time of sending sensory, which ultimately leads to improved postural sensory organizing.
63 The Psychological consequences of abortion , Nadia Saddam Fahad Al-Assady
The number of abortions is increasing every year in USA; while legalization laws of abortion voted in Belgium in 1990 and in France in 1975 were intended to facilitate reducing these numbers. Lack of public interest and relative denial of health clinicians for the psychological consequences of abortion are worrying because people living perinatal mourning need to have their word heard and recognized. Early abortion is a condition that a pregnant woman experiences early in pregnancy. This condition causes fear, tension, great anxiety, and fear of being unable to hold her baby. Early abortion is the loss of pregnancy before the twenty-four weeks of pregnancy and this situation affects about twenty percent of pregnant women. There are many reasons for pregnancy loss and early abortion, some related to the fetus, others to the pregnant mother
64 Evaluation of Gene Xpert/MTB RIF assay for diagnosis of extrapulmonary TB at a tertiary care centre in south coastal Karnataka , Kiran Chawla, Ajay Kumar, Vishnu Prasad Shenoy
Objectives: Very few studies in India have evaluated the performance of GeneXpert MTB/Rif assay for diagnosis of extrapulmonary tuberculosis thus in the present study we made an attempt to evaluate the performance of GeneXpert MTB/Rif assay for routine diagnosis of extrapulmonary tuberculosis. Methods: A total of 441 extrapulmonary samples (188 biopsy, 123 body fluids, 80 CSF and 50 pus) obtained from patients suspected of extrapulmonary tuberculosis were tested for smear microscopy, GeneXpert MTB/Rif assay and culture against a composite reference standard and sensitivity, specificity, positive predictive value (PPV) and negative predictive values (NPV) of the assays were calculated using SPSS version 22 for evaluating the performance of these assay. Results: The pooled sensitivity of GeneXpert MTB/Rif assay, smear microscopy and culture against a composite reference standard was found to be 78.64%, 24.27% and 50.49% respectively. The individual sensitivity of the Gene Xpert MTB/Rif assay for biopsy, body fluids, csf and pus samples was found to be 84.62%, 50%, 71.43% and 95.45% respectively. The pooled as well as individual specificity of all the assays in present study was found to be 100%. Conclusion: Gene Xpert MTB/Rif assay offers very high sensitivity and specificity for diagnosis of extrapulmonary tuberculosis especially in tissue biopsies and pus specimens. Gene Xpert MTB/Rif assay seems to be a better tool than most of the currently available laboratory methods for diagnosis of extrapulmonary tuberculosis. Further longitudinal studies are required to evaluate the feasibility of this assay in local health care settings.
65 Determinatio of biochemical parameters and some trace metals in sera of patients for renal failure disease by spectrophotometry , Hussein H Kharnoob, Salwan K Ahmed
This research was conducted on patients in the hospital of salah al-dine in Tikrit city from 1/11/2016 to 1/5/2017 to determine trace of heavy metals such as Zinc, Copper, Cadmium and lead; and the biochemical changes urea, creatinine ,uric acid and electrolytes (Na+, K+, Ca++ ) in sera of patients with renal failure disease before and after dialysis using enzymatic colorimetric method and graphite furnace atomic absorption spectrophotometer for determination of analysts in serum respectively. The Study involved 52 patients suffering from renal failure disease and their ages was between 17--74 years from the two genders, healthy person were used also as control groups for comparison before and after treatments. The results obtained show that high significance increment with p
66 Cone beam computed tomography (CBCT) in dentistry- students’ and interns’ perspective in Abha, Saudi Arabia, Hussain Almohiy, Ibrahim Alshahrani
AIM: To assess the perspective of dental students and interns towards various aspects of Cone Beam Computed Tomography (CBCT). MATERIALS AND METHODS: A cross-sectional study was carried out among 5th year students, final year students and interns of College of Dentistry, King Khalid University, Abha. A convenient sample of 380 students was taken for the study. A valid, reliable slightly modified questionnaire (Appendix 1) having 11-items was included in the questionnaire. The two sections of the questionnaire consisted of questions related to demographic data such as gender and academic level in the first and questions related to Cone Beam Computed Tomography (CBCT) in the second section. Students who were aware of CBCT use in dentistry were included in the study whereas those unaware of its use were excluded from the study. RESULTS: When subjects were asked whether they were aware of Cone Beam Computed Tomography (CBCT) use specifically for dentistry; 68% were aware while 32% did not know about it. The findings of our study showed that 49.48%, 58.7% and 80% of the 5th year, final year students and interns, respectively; believed that lower radiation dose was the most important advantage of using CBCT over medical CT. Majority of the study subjects would choose CBCT in procedures like implants, extraction of impacted teeth, evaluation of patient’s cysts/tumors and orthodontic assessment. On comparing the responses of the three groups, a statistically significant difference was found. CONCLUSION: Although a majority of students and interns were aware of the use of CBCT in dentistry still 32% were unaware about it. CBCT is a potential imaging modality to bring a new era in the world of imaging science. It has many advantages over medical CT as it overcomes many limitations of the same.
67 Role of Computed Tomography in diagnosis of complications in chronic suppurative otitis media, Osamah Ayad Abdulsattar, Safaa Hussein Alturaihy, Azal Ali Hussein
Chronic otitis media is chronic inflammation of middle ear cavity, it present with longstanding discharging ear and perforated tympanic membrane with or without cholesteatoma and other complications. Cross sectional study was performed to identify the role of computed tomography in diagnosis of complications in chronic suppurative otitis media among 40 patients between June 2018 and June 2019. Aim: To identify role of CT scan in diagnosis of anatomical and pathological effects of chronic suppurative otitis media as: pneumatisation of mastoid, soft tissue mass in middle ear cleft and mastoid, cholesteatoma, bones erosions, facial nerve canal dehiscence, labyrinthine and semicircular canal fistula.Patient and Methods: 40 patients included in our study between period June 2018 to June 2019, they are referred from otology department to radiology department in AL Hillah General Teaching Hospital that thin slices CT temporal bone was taken for all these patients. Results: Age incidence: between 15-20 years was 3 of 40 patients (7.5%), 20-40 was 25 (62.5%), 40-50 was 12 0f 40 patients (30%). Mean age is 32.77 years. 16 of 40 patients were female (40%), 24 of 40 patients were male (60%).Male to female ratio 1.5:1. Incidence of symptoms and signs was: headache & pain in 6 patients (15%), vertigo in 5 (12.5%), hearing loss in 30 (75%), discharging ear in 40 (100%), and facial palsy in 2 (5%). Pathology in middle ear: Granulation in 20 cases (50%), cholesteatoma in 15 (37.5%), and polyp in 5 (12.5%). Pneumatisation of mastoid bone: well in 25 cases (62.5%), sclerotic in 13 (32.5%), and poor in 2 (5%). Middle ear and mastoid mass: in CT scan 25 cases (62.5%) and in surgery 24 (60%). Cholesteatoma incidence: in CT scan 15 cases (37.5%) and in surgery 15 (37.5%). Incidence of complications: scutum erosion in CT 14 (35%) in surgery 15 (37.5%), ossicular erosion in CT 11 (27.5%), in surgery 8 (20%), facial canal dehiscence in CT 2 (5%), in surgery 3 (7.5%), labyrinthine fistula in CT 2 (5%), in surgery 1 (2.5%), semicircular canal fistula in CT 3 (7.5%), in surgery 2 (5%), tegument erosion in CT 1 (2.5%), in surgery 2 (5%), and tegument tympani erosion in CT 1 (2.5%) in surgery 1 (2.5%).
68 Molecular identification of Brucella abortus and its virulence genes (bvfA, virB, and ure) in infected humans and cattle from Al-Diwaniyah province, Iraq, Jameela Radi Esmaeel
Objectives: The current molecular study was launched to detect the existence of Brucella abortus and its virulence genes (VGs), bvfA, virB, and ure, in human blood and cattle milk. Materials and methods: According to that aim, 35 human-blood and 50 cattle-milk samples were collected from Al-Diwaniyah Teaching Hospital and local herd fields, respectively, from Al-Diwaniyah province, Iraq. A polymerase chain reaction (PCR) technique was employed to determine the identity of the bacterium and the presence of its VGs in the samples. Moreover, a partial gene sequencing (PGS) method targeted the 16S rRNA gene was used to confirm the infection by B. abortus in the samples. Results: The results of the PCR demonstrated that B. abortus was detected in 8 (22.9%) of the human-blood samples and in 9 (18%) of the cattle-milk samples. Furthermore, the PCR showed the existence of the bvfA, virB, and ure genes in 4 (50%), 3/8 (37.5%), and 4/8 (50%), respectively, of the 16S rRNA-gene positive PCR blood samples. In addition, the PCR revealed the presence of the bvfA, virB, and ure genes in 2 (22.2%), 5 (55.6%), and 3 (33.3%), respectively, in the positive 16S rRNA-gene-relied PCR from milk samples. For the PGS that employed 2 blood and 2 milk of the positive 16S rRNA-gene-relied PCR samples, the results revealed four different sequences close nucleotide matched up, ~99.6% for the blood and 99.8% for the milk samples, to a world isolate of B. abortus, CP023308.1, from Caserta, Italy. Conclusion: The study shows the existence of the B. abortus in the blood and the milk samples of the studied humans and cattle, respectively, with confirmation that the detected strains were from the virulence bacteria due to the presence of the bvfA, virB, and ure virulence genes.
69 Evaluation Esthetics Smile for Male and Female who attend to Outpatient clinic in Babylon Dental College., Ameer Al-Ameedee
Objective: The current investigation was proposed to determine the analysis smile estheticsfor male and female who attended to outpatient clinic in Babylon Dental College. Materials and Methods: Twenty-six samples ideal images were used in a survey and graded according to smile attractiveness by the male (𝑛 = 35) and female (𝑛 = 47) all groups with age (30-35). Ideal photographs of gingival display, midline diastema, central incisor crown length, and lateral incisor crown width were manipulated with five minor changes in each. For smile arc and buccal corridor, two major changes were incorporated besides the ideal photograph. One-way ANOVA and Post Hoc analysis (Bonferroni method) of the responses were measured for each group. Results: this study found that the smile in male category was the more variations in the smile arc and buccal corridors, with either 0.5 mm of alterations or completes absence in a gingival display. The female were more in variables like buccal corridor, Gummy smile and Lateral incisor crown width. Changes in lateral incisor crown width were not perceivable by the two groups. Conclusion: The degree of perception of smile esthetics to be attractive varies between male and female. The female were more in variables like buccal corridor, gummy smile and lateral incisor crown width. However, some of other variables did not affect the male and female much, with conditions of this study the result showed, the esthetic dentists to be more conscious about alterations in elements which used in this study, each representing an altered smile feature in their smile. Hence, to pay attention to these factors during any esthetic treatment.
70 BIOCHEMICAL AND MOLECULAR MARKERS IN BREAST CANCER PATIENTS, Mohammed J Al-Jassani, Wasan K Alwan, Ayad MJ Almamoori, Maher M Khadairi, Saudi Mohammed Salh
The presents study was designed to evaluate the DNA damage markers, antioxidants makers and lipid peroxidation in breast cancer patients. About 5ml of venous blood was collected from 10 healthy persons and 30 breast cancer patients before chemotherapy. Comet assay used to measure DNA damage and antioxidants were assessed biochemically. Lipid peroxidation was estimated by the Thiobarbituric acid assay. The results of present study showed that the DNA damage markers such as comet length, tail length, DNA percentage in tail and tail moments were significantly increased at p
71 Breast lump, a clinico–histopathological study among women in western Iraq, Tarek Mahdi Salih
Introduction: Breast lump remains a health problem that dreads women all over the world because of its associated idea with breast cancer. As reported by WHO 60-70% of the breast lump were found to be carcinoma cases. Patients and methods: This study conducted in Al- Ramadi teaching hospital from July 2013 - January 2015, all female patients with history of breast lump, were clinically examined and considered in the study accordingly. Excisional biopsy were done for all patients and sent for histopathological investigations for further evaluation. Results: Fibroadenoma was accounted for the highest frequency among all other histopathological findings. By considering malignant cases, no case was seen at stage zero of the disease. Conclusion: All cases of malignant breast tumor showed nipple retractions. No one of the malignant cases was found at stage 0 of the disease, all of them started from stage II. This is actually not a good indication which in turn means that patients lacking information regarding their general health status.
72 Conjunctival-Limbal Autografting Surgical Outcome for Challenging Pterygium Cases: A Prospective Study, Zeena Adnan Abd
Introduction: Pterygium is a common degenerative conjunctival disease, for which several surgical techniques were adopted, but none proved to be ideal because of the relatively high recurrence rate after removal. Objective: the primary outcome measure was to assess the recurrence rate of pterygia after surgical removal with conjunctival-limbal transplantation; secondary outcome measure was to assess safety of the procedure. Method: study design: a prospective interventional study Selection of 20 cases of advanced fleshy pterygia with at least 3.5 mm horizontal extension on the cornea, some primary and some with multiple recurrences, surgical removal with conjunctival-limbal autografting was done Then postoperative fellow up for 3-6 years checking: 1-evidence of recurrence. 2-evidence of limbal stem cell deficiency. Results: mean age was 53.45+- 15.78 year After surgery, the minimum fellow up period was 3 years for primary pterygia, and 6 years for recurrent pterygia NO recurrence was observed during that period; in addition no signs of limbal stem cell deficiency were seen. Conclusion: Surgical removal with conjunctival-limbal transplantation proved to be safe and effective for both primary and recurrent pterygia, with NO recurrence rate.
73 Evaluation of Micronuclei in RadiationWorkers Chronically Exposed to Low Dose Ionizing Radiation, Zahraa Abbas Rasheed, Abdulsahib K Ali, Altaf Abdul Majed
Introduction: Exposure to ionizing radiation causes damage to living cells, especially to DNA, the degree of cellular damage depends on the amount of radiation administered.Humans are naturally exposed to ionizing radiation from cosmic rays, and artificially through diagnostic procedures, medical treatments or occupationally during work shifts. The CBMN assay in peripheral blood lymphocytes are standard method for monitoring chromosome damage in human populations and represents a biomarker for evaluation on human subjects, post-acute or chronic radiation exposure.The present study aims to use the micronuclei analysis as biomarkers for detection of the effects of ionizing radiation exposure inradiation workers chronically exposed to low dose ionizing radiation inAl-Tuwaitha nuclearsite. Material and Method:This study including 50 male blood samples, aged (25 - 58year), as well as 50 male blood samples, aged (25 -58 year) which are non- smokers and non-alcoholics as control group .Data about workers characteristics and habits, received annual doses , total years of service were taken from each subject. Blood samples were taken and micronuclei (MN) number in peripheral blood lymphocytes was calculated using CBMN assay according to standard protocols. Results: The present study showed significant increase (p < 0.05) in the MN frequency in binucleated lymphocytes of the radiation worker as compared with the control group.CB cells lymphocytes having one, two and three micronuclei are rendered evident to all radiation workers and some control group subjects. The micronucleus frequency was found to increase systematically with donor age. After correction for the age-dependence, could be observed. Compared to the group of radiation workers receiving doses below 1 mSv/year, limit recommended by the ICRP for public exposure Conclusion:The results indicate the cumulative effect of low-level chronic exposure to radiological materials. The current results offrequencyMN withinnormal valuesaccordingof the technical reportofInternational Atomic Energy Agency (IAEA)No.405, 2001
74 Association of Epstein- Barr virus (EBV) with development of Hodgkin lymphoma (HL) in western Region of Iraq: Unmatched Molecular case-control study, Mothana Ali Khalil, Mohammed Tafash Dagash, Arkan Jassim Obaid Al-Essawi
Background: Hodgkin's disease is a malignant disease in the blood cells, especially the lymphatic ones, and accounts for 30% of the lymphomas. The Epstein-Barr virus (EBV) is associated with a proportion of cases of Hodgkin lymphoma (HL), and this association is believed to be causal. In Iraq, there are very few studies on this subject. Objectives: The purpose of the current study was to determine the association between EBV infection and Hodgkin lymphoma of Iraqi patients. Patients and Methods: Unmatched case-control study was conducted 55 paraffin-embedded tissues of HL of Iraqi patient’s in addition to 110 normal noncancerous nasopharyngeal biopsy samples as control group. DNA of EBV was extracted from both controls and neoplastic tissues and analyzed by PCR technique using primers specific to EBV Latent Membrane Protein-1 Oncogene (LAMP-1) for the presence of EBV. Results: This study has shown that EBV were found in 51/55 Hodgkin lymphoma cases (92.73%) and in 85/110 controls (77.27%). This study has identified Gender of HL cases was not risky and not significantly associated with illness ((OR 1.00, CI 0.5220–1.9157, P. Value = 1.000). The results of this investigation show that exposure to EBV was risky and independently associated with HL (OR 3.7500, CI 1.2345–11.3916, P. Value = 0.016). Conclusions : The results do support an association between EBV infection and HL.
75 Association of Polyomavirus BK Virus with Chronic Renal Failure patients, Sattar G Ajeel, Asmaa B AlObaidi, Zena M Qaragholi, Haidar A Shamran
Background: Patients in end-stage renal disease (ESRD) were considered immunocompromised, especially those on hemodialysis (HD) procedure which seemed to produce alterations of the immune status. BK polyomavirus (BKV) was found to be an aggravating factor of renal failure, and interest in immunosuppression has increased due to BKV infection. Objective:This study aimed to investigate thepossible association between BKV with ESRD with and without dialysis. Subjects and Methods:A case-control study included 150 serum samples collected from 50 patient with ESRD on dialysis,50 without dialysisand50 apparently normal subjects with normal renal function as control.Quantitative Real time PCR(RT-PCR) was done for detection of BKV viral load. Results: twenty (40%) out of the 50 dialysis patients were positive for BKV by RT-PCR and, eight (16%) were positivein patients without dialysis, while none of the controls was positive. There were highly significant differences (p
76 Effect of Acute Toxicityof Glyphosate in Gold Fish Carassiusauratus, Jenan M Al-Kawaz
The present study deals with the effect of herbicide glyphosate on goldfish Carassiusauratus .Fish were exposed to different concentrations of the mentioned pesticide, the percentages of mortalitywere increasing with the increase of the pesticide concentration. Median lethalconcentration (LC50) of pesticid was calculated after an acute exposure period (96) hours (14.55) ppm. The result showed that theglyphosate was observed mortality in fish during (24) hours in concentration (16, 18) ppm, whereas mortality was not observed during (24) hours in concentration (12,14) ppm. After (24) hours the percentage of mortality was increasing gradually with the increase of exposure time. It is found that the increasing of the concentration of pesticide under study correlated reflexive with (LT50) of these concentrations. The concentration (12) ppm recorded higher LT50 (562) hours , whereas the concentration (18) ppm recorded lower LT50 (33) hours. Behavioral , morphological and histological effects of various concentrations of glyphosate on gold fish Carassiusauratusinthe acute exposure (96) hours were investigated .There were behavioral and morphological changes on the exposed fishes represented by nervous symptoms , loss of equilibrium , increas in respiration rate , opening of mouth and body bend in caudal region(curvature in vertebral column).Histological changes were determined in gills after acute exposed such as hyperplasia , hemorrhage , aneurysms , separation of epithelial layer , clubbing with sickle lamellae , hypertrophy , destroyed and fusion of secondary lamellae.
77 A descriptive cross-sectional survey among the International Islamic University Malaysia’s student on e-waste generation and public health problem in Malaysia, MA Islam, AK Azad, NK Sunzida
Objective: The main aim of the survey was to assess the respondent’s opinion on the factors influencing the generation of e-waste and its impact on public health in Malaysia. Methods: The research project involved a cross-sectional survey of International Islamic University’s students conducted in 2017 at Kuantan campus in the Pahang state, Malaysia. Along with info on demographic individualities, data were collected on several factors (agreement with questionnaire items regarding) using a 4-point Likert scale, with 4 representing “agree”, “strongly agree”, “disagree” and “strongly disagree,” respectively. Results: A total of 296 students completed the questionnaire. The age group of 18-22 covers 46.96% of all respondents and 61.82% was females. In ethnicity of respondents, 86.82% Malay and 30.07% were from rural area and rest of them were urban area. The volume of e-waste is raised due to the excess supplies of electrical appliances (71%), update their devices (65%.), additional electrical devices (59%), keeping the unused electrical appliances (71%), wholesalers selling bad quality appliances (45%), mechanic should not add and drop of their devices (71%). Regrettably 83% of the respondents doesn’t have appropriate knowledge of e-waste. 51% students agreed and 38% strongly agreed that there is no noticeable campaign on e-waste for public awareness. According to the 93% students’ opinion, individuals, the suppliers, industries and even the institutions are not aware to reduce e-waste. But a little 20% think that government does provide facilities for electrical device disposal. In public health perspective, e-waste contains a lot of hazardous chemical (strongly agreed 77% and rest 23% agreed). Fortunately, the serious health hazards led by e-waste (86% strongly agreed and 14% agreed). E-waste leads to infertile land, cancer, lung diseases and so others strongly agreed 70% and agreed 22% respectively. Conclusion: Findings suggest to organize the awareness programme in institutional, organization and educational institutional level to reduce generation of e-waste and its risk.
78 Nano fertilizers and their bioapplications: Mini Review of the literature, Samah Saleh Al=Shybany
Monthapiperita L. An evergreen herb plant with a pungent aroma and belongs to the Umbelliferae family. The mint is 30 to 90 cm long and the wide stems are scattered with fibrous roots. The leaves are characterized by a sharp tip, serrated edges and red veins. They may be slightly iridescent, 9 cm long and 1.5-4 cm wide. The stem is green and the leaves are arranged in pairs opposite and covered with thin hair from one side and the color ranges from dark green to purple and blue and sometimes light yellow and blue flowers and grouped in clusters and other side. Nano fertilizers are the science that deals with matter at the scale of 1 billionth of a meter and are also the study of manipulating matter at the atomic and molecular scale. Recently particulate systems have been used as a physical approach to alter and improve the quality of human life.
79 Role of D-Dimer Test In β-Thalassemia patients, Shrooq M Al-Saleh, Haider Salih Jaffat
Background and aim: β-Thalassemia is hereditary blood disorder that can cause deficient or absent synthesis of beta globin chains, leading to excess alpha chains. Thalassemias are prevalent throughout the Mediterranean region Africa, the Middle East, the Indian subcontinent and South-East Asia. The D-dimer is a dependable and sensitive key of fibrin deposition and steadying. In this way, its available in plasma should be reveal of thrombus formation ,There are many circumstances unconnected to thrombosis in which D-dimer level are high, It is oftentimes used in the estimation of acute venous thromboembolism (VTE) , plus in identification of disseminated intravascular coagulation (DIC) .Regular blood transfusion and compliance with iron chelation therapy has markedly improved life expectancy in thalassemia; however, this improvement is accompanied by several complications of this chronic disease including thromboembolic disorders. The objective of this work was planned to estimate the association between level of D-dimer protein in the blood and beta thalassemia. Materials and Methods: This retrospective, case-control study was conducted on male patients (βT) (n=90) group at aged (4-29 years) and the patients samples collected according to duration of blood transfusion while the control male(n=45) group at the same aged, This study was conducted in thalassemia center in Babylon province, Iraq . The study extended from the start of October 2018 to the end of February 2019.The activity of D-Dimer protein test , Ferritin and hematological parameter (RBCs WBCs, HB, PCV and PLTs) were estimated. Results: plasma D-Dimer levels were significantly higher in BT patients compared to control groups. There were significantly (p
80 Protective effect of Melatonin, Rosuvastatin and their combination against Amikacin induced nephrotoxicity in rats, Hadeel Yousif Noori, Abdulkareem H Abd
The current work was conducted to study the possible protective effect of melatonin, rosuvastatin and combination of them on nephrotoxicityinduced by amikacin in rats. Forty adult male albino rats were allocated into five groups (8 animalseach) and were treated daily for 2 weeks as follows: Group I: :( control group) treated with dimethylsulfoxide (DMSO) orally.GroupII: injected with daily dose of (120 mg/kg/IP) of amikacin ;Group III: injected with daily doseamikacin (120 mg/kg) with daily oral dose of melatonin (10 mg/kg); Group IV: injected with daily dose amikacin (120 mg/kg)with daily oral dose ofrosuvastatin (10 mg/kg);GroupV:animals were injected with daily dose amikacin (120 mg/kg) simultaneously treated with a combination of melatonin and rosuvastatin with the previously mentioned doses respectively. After 2 weeksblood samples were obtained for biochemical analyses. Then, rats were sacrificed and the kidneyswere collected for tissue homogenization and histopathological study. Results: amikacin administration induced significant increase in kidney weight, serumurea and creatinine, tumor necrosis factor (TNF-α), tissue malondialdehyde (MDA) levels and reduction in superoxide dismutase(SOD) activity. Simultaneous administration of melatonin and rosuvastatin treatment with amikacin significantly lowered the elevated serum urea and creatinine concentration, kidney weight, serum TNF-α and renal MDA andsignificantly enhance renal SOD activity with improvement of the kidney histological findings in comparison with group II. Conclusion: it could be concluded that combination of melatonin and rosuvastatin may be useful for reducing the nephrotoxic effects of amikacin.
81 Occurrence of Klebsiella pneumoniae carbapenemase KPC gene in Klebsiella pneumoniaeisolated from patients in Anbar city of Iraq, Mohammed Muklas Ahmed, Safaa Abed Lateef Al Meani
Carbapenems are the last drugs of choice except colistin against seriousinfections caused by Gram-negative bacteria. However, there are increasing number ofreports indicating prevailing emergence of KPC-producingclinical isolates worldwide,Which is of harmful genes because many plasmids that carry KPC resistance elements concurrently carry other plasmid-mediated resistance elements, such as quinolone (QnrA and QnrB) and aminoglycoside (rmtB) resistance . This study reports KPC for the first time among Klebsiellapneumoniae from hospitalized patients in Anbar, Iraq.Six clinical isolatesof K. pneumoniae resistant to carbapenem from 50 isolates were investigated from burned wounds,sputum,C.S.F, and blood samples.The susceptibility to different antibiotics was tested byVITEK-2 system. Where the percentage of resistance to Carbapenemswas 22%. The phenotypic detection of carbapenemases by Modified Carba NP, Blue-CARBA Test that all isolates arecarbapenem gene-producing,5/6 (83.33%) gave positive result with the imipenem-EDTA test,andmodefiedHodg test.The blaKPC and other genes weredetected by multiplex PCR and the result showed 1/6 (16.67%) strains positive forblaKPC gene and 5/6 (83.33%) strains harbored blaVIM and blaOXA-48 genes. Our results showed thecoexistence of both blaVIM and blaOXA-48 genes in four strains of K.pneumoniae,while indicated widespread KPC, VIM,and OXA-48 in Anbar, Iraq. Hence, it is necessary to followproper infection control practices and physicians should be aware of the patients withsuch risk factors.
82 The impact of an innovative device to break the defence of the roller catch for young Romanian wrestling players, Ghazwan Karim Khudair, Riyad Jumaa Hassan, Abdulhussain Majid Muhammed
One of the most important catch in the Roman wrestling grabbed the rollerbecause of its obvious effect during the descent and the importance of this catch through the fact that the player takes the position of the Hack during the game of passive or when getting a mistake where the player sit in the area of the Hack and the player to attack the striker to perform the catch and that the catch a set of and of the alternatives for the purpose of getting the defender out of the situationthe danger in the application of the catch and give points, and the importance of research in the manufacture of an auxiliary device through which to break the defense of this catch and thus take the points and win the landing as this device can be circulated to the rest of the age groups and teams of women, hence the problem of research that the player wrestling during theapplication of Roller Bearing is a set of alternatives to the grip, including rotation of the body in opposite direction of the player. So that it hinders movement and therefore not to give the points focused the problem of research by the following question (How to break the defense of the roller handle)?the purpose of the research (designing an innovative device and knowing its effect in breaking the defense of the roller catch,that the innovative device has the effect of breaking the defense Grab roller).
83 Muscular balance of the upper limb and trunk and its relation to the performance of certain skills in gymnastic , Abdul Al-Hussein Majid Mohammed Al-Jashmi, Hamid Nouri Ali, Ghazwan Karim Khedair
importance of research lies in determining amount of strength between the muscles as well as muscular balance between the both limbs of the player and the one limb and between the trunk and abdomen and the importance of research in identifying an important aspect and attention toward the distribution of nerve signals between the limbs of the body and between working and counteracting muscles, the search problem summarized is that most trainers train some muscles with more attention than other muscles such as agonist or antagonistic muscles and this affects the muscular balance especially between the agonist muscles in the performance of the athlete, which makes the performance and the movement do not go smoothly correctly. The aim of the research was to identify the correlation value between muscle strength of the limbs and the balance of the limbs of the body and the skill of jumping the hands and jump the hands on the jumping platform. The research community included Al-Qadisiyah University's players in Gymnastics for the academic year (2017-2018). There were 10 players representing the research sample. Photography of technical performance of skills 1- Evaluation of the performance of the hand jump. 2- Evaluation the performance of the jump of the hands on the jumping platform. The researchers photographed the players when the two skills were performed on the gymnasium hall and then the video was shown on experts and specialists in the Gymnastics field and the tests were then conducted to evaluate the muscle strength of the body parts as follows: 1- Biceps muscles of the arm (right, left) 2- Half Squat muscles of the arm(right ,left) 3-Muscles of abdominal flexion 4-Muscles stretching back Data were statistically processed by extracting the value of the correlation coefficient between the variables of the study and through it we can conclude the following: 1 - All the muscles of the players of the University of Qadisiyah (under study) in Gymnastic balanced whether between the left and right limbs or one limb of the body (Biceps - Half Squat) as well as the muscles of the trunk. 2-The strength of the back muscles have a big role in the performance of the skill of the hand jump in the players of the University of Qadisiyah team in Gymnastic.
84 Correlation of Antisperm Antibodies with Trace Elements in Seminal Fluid of Immunologic Infertile Men, Abdul Kareem M Jewad, Ehab Rasmi Alkhafji, Naael H Ali
Immunological cause may contribute to 5-15% of the male infertility factors, including cryptorchidism, primary testicular failure, testicular trauma, epididymitis, varicocele, idiopathic infertility, and infections. Therefore, ASA can be found in primary or secondary infertile men. The aim is to study the correlation of ASA with trace elements in primary and secondary infertile men. The study was carried out in the Department of Medical Laboratory Technology in Southern Technical University. Seminal fluids were collected from each of them, seminogram test (semen analysis) was performed for each participant, and all semen samples were collected following (3-5) days of abstinence. After liquefaction, sperm concentration, total sperm count, morphology, motility grades were determined using World Health Organization (WHO) standard procedures. The mean values of were significantly increased ASA in infertile men as compared to fertile control group (p
85 Histological study of testis tissue of male golden hamster (Mesocricetusauratus) in different time points of Diabetes, Manar Mohammad Hasan Al-Murshidi
Previous studies have reported that hyperglycemia can induce cell death in many tissues like brain, liver, kidneys, and testis. Recent studies have indicated that diabetes can trigger male infertility. In this study we explain the histological analysis of testicular tissue after induced diabetes in hamsters. Testicular tissues were examined in the control group and in diabetic hamsters groups at different time periods after diabetes induction. Hyperglycemia was induced in experimental male hamsters by intraperitoneal injection of streptozotocin drug (STZ). At different time points (4, 8 and 20 weeks) after diabetes induction, hamsters were euthanized and the testicular tissues were removed for the histological analysis. Testis tissues were fixed in formalin (10%) and then processed for histological analysis, and examined under light microscope. The histological results showed reduction in cell density in the testis, which indicated that diabetes and hyperglycemic conditions defect normal cell density in the testis tissues. The testicular histology of the diabetic animals showed acute reduction in cell density occurred after 20 weeks. In conclusion, the induced diabetes condition provides evidence that hyperglycemia plays an important role in the pathogenesis of diabetes, and it also indicated that chronic hyperglycemia eventually leads to cell death and then male infertility.
86 Study of some parameters of pregnant women with diabetes as well as anemia, Khalid abdulkareem Mohammad
In the Yarmouk hospital laboratory, 120 blood samples were withdrawn from pregnant women with diabetes and 55 blood samples of them are pregnant women with diabetes and anemia, according to the methods used in the analysis. Results were recorded and fixed in figures for the purpose of distinguishing between the vital functions to study and recommendations which helps pregnant women to prevent diseases and to be carefulto health culture for motherhood and free childhood from the diseases. The study shows hemoglobin levels in the blood as well as the level of blood sugar for women who are normal and who have anemia as well as diabetes.
87 Electronic study of fresh enzyme complexes of antifungal drugs-P450 and Aspergillus kojic acid biosynthesis. W: w saccharose flavus: fructose as a substratum, Khansaa Basem Fadhil, Moazaz Abd Alrida Majeed, Mohammed Ahmed Mustafa
Aspergillus(A) flavus cell contain the natural product cytochrome p450 enzyme, which is made up of from 400-500 amino acids and containing single heme group with single ferric ion, this enzyme destroyed by and reacted potentially with the used antifungal structures to yield new complexes with different electronic stability which related to the quality of the drug. The new complexes were studied by spectrophotometric technique. The theory on application it on the complexes indicated the following: low spin (LS) complexes with high crystal field stabilization energy (CFSE), octahedral shape and paramagnetic. Calculation and discussion pointed that the p450-triazole complex is the most stable and have higher stabilization energy. A flavus was grown on a carbon source formed from a combination of 1w:1w sucrose: fructose substrate to produce kojic acid by shake flask fermentation. The acid is detected and then identified by its physical properties as yellow crystalline material, odorless, melting point (m.p.) = 155-158(°C), lambda λmax=272nanometer (nm) in distilled water as pure solvent.
88 RFLP- PCR for identification of dermatophytes and Candida species from clinical isolates of hair and skin, Karrema Al-khafajii, Kawther MA Hasan, Rsinula D Abdul Moh
The identification of hair and skin agents is important in order to define clinical treatment. However, in most cases conventional culture identification can be considered to be time-consuming and not without errors. The aim of the present study was to identify the dermatophytes and Candida spp. isolated from hair and skin lesions by using the polymerase chain reaction and restriction fragment length polymorphism analysis (PCR/RFLP). Fungal universal primer pair (ITS3/ ITS4) was able to amplify internal transcribed spacer 2 (ITS2) region of rDNA. However, three restriction enzymes were used to digest the PCR products: HaeIII, MspIand Corf. Twenty fourisolates of dermatophytes and Candida spp. identified based on their PCR/RFLP pattern. Our study of PCR/RFLP method easily differentiated dermatophytes and Candida spp. and this result was also explained genetic intra-evolution for these isolates.
89 Association of Cortisol with severity and duration of depression in male and female, Meena M Abdul-Hussein, Noor K habash, Mahir M Alramahy, Noor M Shamkhi
Introduction: Depression is associated with increased serum Cortisol. Many parameters affect the cortisol changes during depression such as gender of patients, severity and duration of disease which might be directly or inversely proportional with Cortisol. Aim of study: to assess the correlation of serum Cortisol level with depression severity and duration in both genders of depressed patients compared with healthy controls. Patients and method: a case−control study included thirty three in/out patients diagnosed as mild, moderate and sever depression , they were allowed to use their medications during the study, (24 males, 9 females) and thirty five subjects with out depression as healthy controls (24 male, 11 female). Serum Cortisol level was measured by ELISA (Enzyme Linked Immuno Sorbent Assay) technique. Results: serum cortisol level increases significantly in patients with depression compared with control group(P ≤0.001), with significant correlation with the severity of depression(P= 0.01), there was higher level of cortisol in patients with short duration of disease which is less than two years than those with long duration or chronic for two years and above(P≤ 0.05) . In female depressed patients, cortisol was significantly higher than male depressed patients (P ≤ 0.001). Conclusion: the study found that serum cortisol correlates positively with severity of depression, In long duration of depression the cortisol level is less than shortduration, The cortisol level is higher in female depressed patients than the male patients
90 Histopathological study of hepatic and pulmonary sheep's hydatidosis, Israa Kasim Al-Aubaidi, Ali N Yaseen, Maha A Gathwan
Echinococcosis or hydatid disease is caused by infection with cestoda tapeworm belonging to the genus Echinococcus. It caused public health and economic problems worldwide. Different species responsible for this disease include E. granulosus, E. multilocularis and E. vogeli , etc. Most sites of infection are liver and lung with slow development of the cyst (larval stage), causing many pathological effects that occur in these organs. This study was aimed to investigate different histopathological changes in the liver and lung of sheep's infected with hydatid cysts. Many histopathological variations were noticed in the liver that infected with E. granulosus cysts included hepatocytes degeneration , nuclear changes , necrosis and a steatosis on infected liver sections. Fatty changes also noticed. Increasing in kupffer cells with participation recorded in most sections. Lung sections showed extensive tissue reaction accompanied with inflammatory cells infiltrate around the necrotic areas. Sections also revealed formation of granuloma compact fibrous structure of hydatid cysts and fibrin rich exudate cause hyaline formation
91 Clinical and MRI evaluation of degenerative lumbar spine stenosis, Abbas Abd Zaid Barrak
A prospective study conducted on 50 patients with degenerative disease of lumbar spine complaining of lower back pain and/ or sciatica, those evaluated by detailed history, physical and radiological examination with special emphasis placed upon MRI finding.The pattern of clinical presentation analyzed and correlated with the different MRI findings. Age range between 27- 72 years, the highest proportion of patients age was 50- 59 years with predominance of males (our patients sample consists of 28 men and 22women). The most common clinical presentation was intermittent neurogenic claudication (in 52% of our patients) and more common among elderly patients or patients having multilevel stenosis, while radiculopathy symptom was more common among young patients or patients having single level stenosis. Chronic caudaequina syndrome was uncommon pattern of presentation with bladder function most seriously affected function
92 The anti- Leishmaniasis activity of green synthesis silver oxide nanoparticles, Hawraa H Ismail, Salah Abdu Hasoon, EntsarJ Sahebllah
The green biosynthesized nanoparticles are hydrophilic, biocompatible, and non-toxic and have an important application in different field of science. Leishmaniasis is a protozoan vector-borne disease and is one of the biggest health problems of the world. The anti-Leishmanial drugs have toxicity and the recent development of resistance. In the present study, Ag2O NPs was synthesized using Ficus benghalensis prop root extract (FBPRE) and used against Leishmania (L) donovani. The UV–visible adsorption spectra showed that the absorbance peak is in the range of 430 nm, the vibrational modes of phytochemicals in the extract have been characterized by technique FTIR which allow the identification and information about material. TEM, SEM and XRD pattern have been used to confirm the morphology of silver oxide, which have a spherical nanoparticles shape, and crystalline size of 16 nm. Diluted concentration (25, 50, 100, 200, and 300 μg/ml) of Ag2O NPs were used against L. donovani. The result indicated the effect of Ag2O NPs on the parasite growth rate which clearly decreased compared with L. donovani treated with the same concentrations of the standard anti leishmanial drug (pentostam drug) and the control group. The viability percentage decreased to 66.3 ± 5.44 % compare with group that treated with the pentostam which was 270.0 ± 4.33 in 300μg/mL concentration after 72 hours. The current results concluded that Ag NPs had an effect to inhibit L. donovani growth in vitro following the infection with parasite.
93 Bardoxolone Ameliorates Cerebral Ischemia/ Reperfusion Injury in Male Rats, Rihab H Al-Mudhaffer, Laith M Abbas Al-Hussein, Saif M Hassan, Najah R Hadi
Background: Ischemic cerebrovascular disease causes oxygen deprivation to the brain tissue. Reperfusion, which can occur spontaneously through dissolution of the thrombus or by therapeutic recanalization, leads to the generation of reactive oxygen species (ROS)and induces inflammation which further damages the brain tissue. Methods: Animal model of rats were subjected to general anesthesia during which an occlusion of bilateral common carotid arteries was done for half hour then reperfusion for one hour with and without prior treatment with Bardoxolone 3 mg/kg intraperitoneal (i.p) 24 hr. Brain tissues were sent for histopathological scoring assessment as well as for estimation of Nrf2, NF-κB and MDA levels. Results: The level of nuclear Nrf2 was increased significantly (P
94 The Potency of a Combined Diclofenac-Ampiclox against Streptococcus Pyogen Isolated from Patients with Pharyngitis: A pilot study, Rana Talib Al-Nafakh, Hussein Abdulkadhim, Naser A Naser
Background: Pharyngitis, scarlet fever, and rheumatic fever are of the major infectious problems caused by Streptococcus pyogen. Emergence of antibiotic- resistant strains makes such infection, its rheumatic valvular disease, and glomerulonephritis complications a serious clinical challenge especially in children. Aims and objectives: In a maneuver of overcoming S. pyogen resistance and improve its response to antibiotic, a no steroidal anti-inflammatory drug NSAID is repurposed as antimicrobial against S. pyogen. In intention to be combined with the classical antibiotic recommended by WHO guideline of care. The combination was assessed for its MIC and combination index against S. pyogen. Materials and Methods: A multidrug-resistant strain of S. pyogen was isolated from patients, suffering pharyngitis. The most serious isolate was cultivated for MIC determination of ampicloxdiclofenac combination in comparison with each alone to determine the combination index. Results and conclusions: There was a significant synergism between ampiclox-diclofenac (index ˂ 1) at P= 0.012 and Z score= 2.3 further confirmation of MIC folds of dilutions are to be assessed to obtain reasonable evidence.
95 Cerebro-Protective effect of bosentan in brain ischemia reperfusion injury, Ahmed M Almudhafar, Ahmed J Hussein, Zahraa K Alhassani, Najah R Hadi, Bassim I Mohammed, Dina A Jamil, Hayder A Al-Aubaidy
Background: Ischemia reperfusion injury following acute ischemic insult is responsible for extension of injury. Bosentan is an endothelin receptor antagonist, which is currently used as a strong vasoconstrictor. This study aims to investigate the effects of bosentan on ischemia reperfusion injury after brain ischemic stroke in rat model. Methods: Forty male Wistar rats were randomly allocated into four study groups: Sham group: Rats underwent the anesthesia & surgery for an identical period to the other 3 study groups without intervention. Control group: Rats underwent anesthesia & surgery including bilateral common carotid artery ligation (BCCAL) for 30 minutes and then reperfusion for 1 hour. Vehicle group: Four days before ischemia, rats were administered with a vehicle (5% gummi arabicum) and then anesthesia &BCCAL surgery were performed. Bosentan treat group: Four days before ischemia, rats were administered with bosentan (100mg/ kg/day) and then anesthesia & BCCAL surgery were performed. Results: Bosentan treated group exhibited lower concentration of interleukin 6 (IL-6)in the brain (2168.0±30.67 pg/mL) than in the control group (2571.37±96.58 pg/mL) (P≤0.05). In addition, interleukin 10 (IL-10) levels were significantly high in the bosentan group (275.7±15.97 pg/mL) when compared to the control group (244.05±12.23 pg/mL) (P≤0.05). This was associated with a non-significant reduction in the brain levels of endothelia nitric oxide synthase (eNOS) in the bosentan treated groups (90.23±1.14 ng/mL) when compared to the control group (90.94±2.48), (P>0.05). Conclusion: Bosentan treatment have protective effects against the inflammatory damage following ischemia reperfusion injury following acute myocardial infarction.
96 Incidence of clindamycin resistance among clinical isolates of Staphylococci in a tertiary care centre of Manipur, India, Singh Rajkumar Manojkumar
Introduction: Therapeutic failure of clindamycin against both methicillin resistant and sensitive staphylococcal isolates has been documented as due to multiple mechanisms that confer resistance to macrolide, lincosamide and streptogramin B (MLSB) antibiotics which can be inducible or constitutive. Routine in-vitro antimicrobial susceptibility testing for clindamycin may fail to detect such inducible clindamycin resistance, thus necessitating the need to detect such isolates by employing a simple double disk approximation or D-test. The study was taken up with the objective of determining the incidence of inducible clindamycin resistance among staphylococcal isolates using D test. Materials & Methods: Isolation, identification and antibiotic susceptibility testing of 751 S. aureus and 501 CONS clinical isolates obtained from January 2012 to December 2015 were performed using conventional standard protocol as per CLSI guidelines. Erythromycin resistant and clindamycin sensitive isolates were further subjected to D test for detecting inducible clindamycin resistance phenotype. Results: A total of 385 isolates were found to be resistant to erythromycin and sensitive to clindamycin. 244(19.49%) isolates were demonstrated as inducible clindamycin resistance (iMLSB) by the D test. Conclusion: The study showed the presence of iMLSB resistance among staphylococcal isolates in our tertiary care hospital. Clinical microbiology laboratories should use the double-disc diffusion test (D test) as standard practice with all erythromycin resistant staphylococci to avoid treatment failures of clindamycin
97 Histopathological performance of an HPV18 \ KSHV co-infection with Kisspeptin expression in cervical precancer and cancer tissues, Jasim Mohammed Muhsin, Ameer M Hadi, Sahira Hamdan Abbas
Background: Cervical cancer (CC) is one of the most 12th recurrent neoplasia among Iraqi women turning it into significant health issue, it had been currently supposed that the co-infection of High-risk human papillomavirus (HPV) and Kaposi sarcoma-associated herpesvirus (KSHV) which both share some transmission routes from one side, and the disruption of Kisspeptin expression in cervical tissues from the other side, which are collectivelyact as a causative agents and co-factors of cervical cancer progression. Aim: to compare the infection and \ or co-infection of HPV18 \ KSHV and the dysregulation of Kisspeptin expression between cervical dysplasia (pre-cancerous tissues) and cervical cancer (cancerous tissues) progression. Results: Overall, results showed a significant association between infection and \ or co-infection of HPV18 \ KSHV with both types of test tissues (pre-cancerous and cancerous), although the detection rate of KSHV DNA was very low in comparing with HPV18 DNA in both cervical tissue samples. Furthermore, a significant interaction of Kisspeptin low expression in cancerous samples compared to pre-cancerous tissues with viruses existence seem to involve significantly in Kisspeptin expression of CC. Conclusions: Data suggest KSHV co-infection may act as co-factor in the development of HPV related cervical carcinogenesis. Besides, the defect of low Kisspeptin expression serves as a great candidate for cervical carcinoma progression or maybe as a therapeutic target for CC.
98 Ibudilast and octreotide can ameliorate acute pancreatitis via downregulation of the inflammatory cytokines and Nuclear Factor- Kappa B expression, Alaa Ghazi, Sameer H A Abood, Hassan Alaqouli, Najab R Hadi, Sahar A Majeed, Ali M Janabi
Background: Acute pancreatitis (AP) is severe inflammation of the pancreas that can be of two major types: mild AP and severe AP. In this case, some pancreatic cells can produce various pro-inflammatory cytokines and transcription factors thereby affecting the function of pancreas. Objective: to study the efficacy of Ibudilast in comparison with octreotide, in a rat model of AP. Methods: A total of 48 male rats were randomly divided into 8 groups (6 per each). AP was induced by Larginine model which has a high reproducibility. Octreotide and Ibudilast were administered individually or in combination at 0, 8or 16 hr after induction. After 24 hr of treatment, each rat was weighed and blood samples were collected for measurement of biochemical markers, and then the pancreas was extracted to obtain the pancreatic weight to the total body weight (PW/TBW) ratio and for vertical gel electrophoresis experiments. Results: In both octreotide-treated group and ibudilast-treated group, there were statistically significant decrease in serum pro-calcitonin (PCT) and tumor necrosis factor-alpha (TNF-α) when compared to control group or sham group, as well as, a decrease in PW/TBW ratio and in nuclear factor kappa B (NF- B)protein level using vertical gel electrophoresis. However, in octreotide + ibudilast-treated group there was no statistically significant difference when compared with groups treated with either agents. Conclusion: Ibudilast and octreotide can significantly attenuate the local and systemic effect of AP. The efficacy of ibudilast and octreotide are almost the same. Co-treatment of rats with both Ibudilast and octreotide has no preferential effect when compared with rats treated with the individual agent
99 Early pregnancy vitamin D deficiency and risk of preterm Birth, Noorham Shakir, Ahmed S Al-Hilli, Affifa Hamed Saffa
Background: Many researches done for publication on mineral and vitamins during pregnancy and the possible influences of supplements on pregnancy outcome. Vitamin D may play roles in pregnancy and its complication. Aim: To estimate the association between maternal 25-hydroxy vitamin D concentration in early pregnancy and risk of preterm birth. Sitting: In Al-Zahraa Teaching Hospital in Al-Najaf City from March 1st 2015-September 30th 2015. Type of study: Prospective cohort study Method: 300 pregnant women were included in the study. Blood sample from each case withdrawn from pregnant women 14- 20 weeks of gestation, sent for vitamin D assay. Cases were followed during pregnancy to identify patients who will develop preterm labour. Results: The result of this study showed that 300 pregnant women was with deficient or had insufficient vitamin D level. The relative risk is 6 times which mean that those who had deficient or insufficient vitamin D level 6 times more likely to end with preterm delivery. Conclusion: In this study found that there is strong association between.
100 Fibroblast growth factor 21 as a predictor of early stage diabetic nephropathy in type 2 diabetic mellitus, Samah Naji, Kareem Ghali, Najah Hadi, Maysaa Ali Abdul Khaleq, Mohammed Abdelhussain
Elevated fibroblast growth factor 21(FGF21) was suggested in cross-sectional studies as an indicator of subclinical diabetic nephropathy. We investigated whether serum FGF21 was predictive of the early stage of diabetic nephropathy. The present paper aims mainly to demonstrate the correlation of FGF21 with the degree of proteinuria in type 2 diabetic patients (T2DM). One hundred twenty four participants diagnosed with T2DM were recruited and they were divided into three study groups according to their albumin/creatinine ratio (ACR): (Normo albumin urea group, Micro albumin urea group, and Macro albumin urea group).Samples of blood and urine were collected to measure different glycemic control and kidney function markers. We conclude that significant correlation between FGF21 with Albuminuria (*p = < 0.001), mean FGF21was highest in macro albumin urea followed by micro albumin urea and lastly Normo albumin urea.
101 The effects of the nanosphere carrier for the combined carmustine-busulfan trastuzumab in human breast cancer tissue culture, Zena Hasan Sahib, Sarmad Nory Gany Al-Dujaili, Hussein Abdulkadhim, Rena A Ghaleb
Cancer of the breast is from the highest cancer type’s incidence. Cancer in general represents a high therapeutic challenge. Considerable adverse effects and cytotoxicity of highly potent drugs for healthy tissue require the development of novel drug delivery systems to improve pharmacokinetics and result in selective distribution of the loaded agent. Targeted therapy is a novel maneuver to achieve proper selectivity index. And as the main goal of nanocarriers is to target specific sites and improve the circulation time of the drug which is entrapped, encapsulate or conjugate in the carrier system so we chose nanoliposome as a drug carrier. Liposomes improved a potent drug targeting successfully in the last decade, but nanoliposomes offer more surface area and they have more solubility, improve controlled release, enhance bioavailability, and permit precision targeting of the material that is encapsulated to a greater extent. The Aims and objectives of the study is the formulation of HER2 Ab directed nanosphere carrier for a combined Carmustine-busulfan and trastuzumab (LCBT), then Assessing antineoplastic efficacy of (LCBT) in lung carcinoma cell line. A dose-dependent cellular growth inhibition on all three cell lines (P-value < 0.001) was seen. The concentration of 100 µg/ml of LCBT show highly significant inhibitory effect (P-value < 0.001) on cancer cell line in comparison to other concentration and other positive control drugs that forming the combination separately (P-value
102 Evaluation of Some immunological and biochemical parameters as markers in the early diagnosis of diabetic nephropathy, Rawaa Behlul Al-Fatlawi, Kifah Jabbar Al-Yaqoobi, Ameera A Alsadawi, Najah R Hadi, Kareem Ghaly, Wasan Hamid Shukur
The Diabetic Nephropathy(DN) is the major reasons for ESRD in the most common around the world as a result of the rising in the prevalence of obesity and T2DM.The death rate of DN patients having dialysis during 1-5 years is higher than non-diabetic according to the high danger of cardiovascular and infectious diseases. The diagnosis and follow-up of the aggravation of diabetic nephropathy is critical in reducing mortality from this disease. The present study was designed to exam some immunological and biochemical parameters in the early diagnosis of diabetic nephropathy. Four hundred T2DM patients were enrolled, and their age range was 39-60 with a mean± SD of 49.4±10.1 years, they were referred to the Department of diabetic (Alasdair Teaching Hospital) during the period October-2017 to October-2018 for treatment. According to the level of Albuminuria the patients were distributed into three clinical groups to normal Albuminuria (n=145), microalbuminuria (n=162), and macro Albuminuria (n=93) groups. The whole blood of all the patients was withdrawn. The plasma of all patients were tested for HBA1C, Creatinine, Urea, albumin concentration by spectrophotometer, and ICAM-1, TNF-α, IL-1β,IL-6, HMGB1concentration by ELISA technique. The study included the Creatinine values were significantly higher in macro Albuminuria compared with Micro Albuminuria and normoalbuminuria (1.3±0.8) versus (0.9±0.2), (1.0±0.2) respectively. Urea values were significantly higher in Macro Albuminuria and Micro Albuminuria (59.5±14.5), (62.4±22.1). IL-1β values were significantly higher in Micro Albuminuria compared with Macro Albuminuria and Control (43.2±18.8) versus (34.3±25.4), (31.7±19.8) respectively. ICAM-1Avalues were significantly higher in Micro Albuminuria compared with Macro Albuminuria and Control (62.67±5.6), (46.7±5.9),(41.93±5.8), respectively.
103 Effect of genetic variation of TLR-4 gene on immune response of Diabetic patients with normal Albuminuria, microalbuminuria and macroalbuminuria in Najaf Governorate, Iraq, Rawaa Behlul Al-Fatlawi, Kifah Jabbar Al-Yaqoobi , Ameera A Alsadawi, Najah R. Hadi, Mohammad A Abdelhussein, Wasan Hamid Shukur, Kareem Ghaly
TLR4 gene polymorphisms are known to impair corresponding signaling pathways in immune and non immune cells followed by weakened adaptive immune responses. This phenomenon can be seen in several inflammatory diseases such as rheumatoid arthritis (RA), cancer, asthma and for coronary artery disease, atherosclerosis and diabetes. A lot of studies indicated that polymorphisms of TLR4 were correlated with immunity-related diseases, such as chronic inflammation and atherosclerosis diseases. The present study was designed to examine TLR-4 gene polymorphisms Asp299Gly (rs4986791) (+1196C/T)and Thr399Ile (rs4986790) (896A/G) effect on some immune markers IL-1β, IL-6, TNF-α, HMGB1,and ICAM-1 in Type 2 Diabetes Militias patients, inspected two single nucleotide polymorphisms (SNPs) of TLR-4 gene. The study included the TT genotype IL-1β concentration were significantly higher in microalbuminuria (31±13.1) than control group (25.9±3.4) which is lower than Macro Albuminuria group (31.2±12.5), TNF-α concentration in CT genotype were higher in Micro Albuminuria and macroalbuminuria (962.9 ±53.3), (965±51.5) groups respectively than normoalbuminuria group (786.9±567.3). Considered to GG genotype HMGB1 concentration were significantly higher in control group (1193.82±189.69) than Macro Albuminuria group (500.46±423.54), AA and AG genotypes of IL-6 plasma concentration were significantly higher in control group (T2DMNormo Albuminuria) (30.49±7.9,26.97±45.49), (19.45±8.5, 20±6.17) respectively than control group (12.42±6.27,13.5±8.67).
104 The role of Cucumic Melo Varieta Agrestis, Vitamin C and Zinc on Sperm Activity and Oxidative Stress during Cryopreservation in Infertile Male, Mohanad Waled Abd , Estabraq Abdul Rasool Alwasiti , Haider Behaa Sahib , Maysaa Ali Abdul Khaleq
The objective of this study was to investigate the role of Cucumic melo varagrestis, Vitamin C and zinc on human spermatozoa and oxidative stress during cryopreservation in asthenozoospermia. One hundred males were participated in this study at Infertility Clinic at High Institute for Infertility Diagnosis and Assisted Reproductive Technologies/Al- Nahrain University. There ages were (31.86 ±0.76) years. Semen samples were collected and seminal fluid analysis was done according to WHO (1999). Cryopreservation kept for one month in liquid nitrogen (LN2) at (-196◦C 1ml semen sample with 1ml of sperm freeze medium with zinc, vitamin c , cucmic melon extraction). After one month; 0.5 ml of Ferticuilt Flushin was added to pallet and incubation 37◦C\30min and Microscopic sperm parameters Examination had conducted. The oral administration of antioxidant combination of Cucumic melo var. agrestis, vitamin C and zinc improves sperm characteristics in asthenozoospermia.
105 Predictive value of serum LDH of fever duration in properly treated typhoid fever , Ali Fawzi Abdalsahib Al-Zamili
Typhoid fever is associated with a number of biochemical derangements which are non specific. Some of these biochemical tests have been evaluated in typhoid fever. Lactate dehydrogenate serum level was one of these recently tested non specific biochemical indices in association with typhoid fever. The serum level of this enzyme gets increased following tissue damage in a number of medical conditions. Several authors have investigated its role in typhoid fever, but little has been stated regarding its role in prediction of duration of fever in this disease. The Aim of the study: The current was planned and conducted in order to evaluate the predictive value of serum LDH in association with fever duration in patients with typhoid fever on antibiotic therapy. The current cross sectional study was carried out in Diwaniyah Teaching Hospital, Diwaniyah Province, and mid-Euphrates region of Iraq. The study included 51 in patients complaining of typhoid fever who were on antibiotic therapy. The age range of those patients was from 16 to 70 years and the study included 26 males and 25 females. The main outcome was fever duration in days after initiation of antibiotic therapy. Neither ALT nor AST showed significant difference (P> 0.05); however, serum LDH level was significantly higher in patients with fever duration of 5 days or more in comparison with that of patients with fever duration of < 5 days (P = 0.035), 499.50 (369.75) versus 370 (99), respectively. Besides, there was highly significant correlation between serum LDH and fever duration (P< 0.001). The correlation was positive (r = 0.753) revealing that the higher the serum level of LDH is, the longer the duration of fever is. Serum LDH is a significant predictor of duration of fever in patients with typhoid fever following initiation of antibiotic therapy with an acceptable level of accuracy.
106 The Potency of a Combined Diclofenac-Ampiclox against Streptococcus Pyogen Isolated from Patients with Pharyngitis: A pilot study, Rana Talib Al-Nafakh, Hussein Abdulkadhim, Naser A Naser
Pharyngitis, scarlet fever, and rheumatic fever are of the major infectious problems caused by Streptococcus Pyogen. Emergence of antibiotic- resistant strains makes such infection, its rheumatic valvular disease, and glomerulonephritis complications a serious clinical challenge especially in children. Its aims and objectives are, in a maneuver of overcoming S. Pyogen resistance and improve its response to antibiotic, a no steroidal anti-inflammatory drug NSAID is repurposed as antimicrobial against S. Pyogen. In intention to be combined with the classical antibiotic recommended by WHO guideline of care, the combination was assessed for its MIC and combination index against S. Pyogen. There was a significant synergism between Ampiclox-diclofenac (index ˂ 1) at P= 0.012 and Z score= 2.3 further confirmation of MIC folds of dilutions are to be assessed to obtain reasonable evidence.
107 Validity of Ultrasound Shear Wave Elastography and Aminotransferase Platelet Ratio Index (APRI) in Liver Fibrosis, Asst. Prof. Dr. Riyadh W. Al Esawi, Prof. Dr. Nada Rudha Al-Haris, Prof. Dr. Abdulameer Al-Mousawi, Dr. Thanna Naji Shaker Abo Ghanaim
Back ground: Chronic progressive liver diseases cause liver fibrosis which end in result is decompensating liver failure. Liver fibrosis that results from these diseases can be reversed if diagnosed early. The current gold standard in the diagnosis of liver fibrosis is a liver biopsy preferably ultrasound guided, which is an invasive procedure with limitations and risks. Recent research has validated the use of shear wave ultrasound based liver elastography which is a non-invasive imaging based tool that has a sensitivity and specificity that almost parallels histological diagnosis from a liver biopsy. The staging of liver fibrosis at diagnosis uses a Metavir scoring system that has been adapted by elastography AIM: To assess the accuracy of point shear wave elastography with aminotransferase platelet ratio index score in the staging of liver fibrosis in chronic liver disease. Materials and Methods: The study was carried out at Al Ameer Diagnostic Center of Radiology and Gastroenterology Center in Al Sadder Medical City in Al Najaf. During the period between November 2017 and July 2018, 62 patients each of them was examined by 2D point Shear Wave Elastography which is built in Ultrasound Machine Affinity 70 Philips, France with a multiband frequency convex probe. Liver function test with Liver biopsy was performed under ultrasound guidance from right liver lobe, under local xylocaine 2% injection with using 16 gauge co-axial automatic True cut biopsy needles Results: A total of 62 patients had been included in this study, 38 (61.29 %) were males and 24 (38.71%) were females with mean age 44.61± 14.92 years for all patients. Their Body Mass Index was 26.88 ±5.3 Gray scale ultrasound examination of the liver revealed that, the mean of cranio-caudal diameter of right lobe was 129.01± 11.6 mm. Regarding hepatic parenchyma texture, in 48 patients had normal texture, in 13 patients with coarse texture, heterogeneous texture seen in 1 patient. Fifty-five patients had regular hepatic surfaces and 7 patients with irregular surfaces. Portal vein mean transverse diameter in 58 patients was 12.08 ± 1.83 mm, in remaining 4 patients, the portal veins were occluded by thrombosis. portal vein mean velocity was 22.2 ± 5.2 cm/s. The mean of Liver Stiffness Measurement for the studied population was 12. 5 ± 2.07 (mean± Standard Diffusion). Aminotransferase Platelet Ratio Indexmean score for the studied population was 1. 2 ± 0.8. These patients were sub grouped in five categories according to Liver Stiffness Measurement, 8 patients in grade F0 (< 4.6), 14 patients grade F1 (4.6-5.6), 10 patients were F2 (5.7-7.0), 15 patients were in grade F3 (7.1-12) and 15 patients were in grade F4 (>12). While according to histopathological examination, the studied population were categorized as 14 patients F0, 16 patients were F1, 9 patents F2, 1 1patients F3 and 12 in F4. Regarding the Aminotransferase Platelet Ratio Index score the studied patients were classified as grade 0 (1.5) 12 patients. Out of 62 patients enrolled in this study twelve patients were categorized by Aminotransferase Platelet Ratio Index score as F4 with cirrhosis, ten of them were positive in histopathology as F4 and two were negative. while 50 patients were categorized as F0-3 with no cirrhosis, 48 patients were positive in histopathological examination as F0-3, and 2 patients were false negative proved by histopathology as F4 with cirrhosis, so the Aminotransferase Platelet Ratio Index Score Sensitivity was 83.3%, Specificity was 96%, Positive Predictive Value was 93.5, Negative Predictive value was 96% and the Accuracy was 93.5%. Conclusions 1- point Shear Wave elastography is a useful tool to screen for liver fibrosis in the general population during a conventional ultrasound examination, especially when laboratory tests and ultrasound examination are negative despite the potential underlying fibrosis. 2- Gray scale ultrasonography alone is insufficient imaging modality for full assessment of chronic liver disease 3- A simple index like Aminotransferase Platelet Ratio Index, consisting of 2 readily available laboratory results (Aspartame aminotransferase Test level and platelet count), can predict cirrhosis with high degree of accuracy. Besides being non-invasive, it can be determined at the bedside. It can also be of use in areas where facilities for liver biopsy and advanced imaging techniques are not available.
108 Production of Fermented Red Beet Juice using Probiotic Lactobacilli Bacteria, Nehan Bahaaldden Jafar, Zahraa Talib Ghaleb, Zainab Hussein Fadhil
During this experiment, the juice of red beets were extracted and fermented by two species of probiotic bacteria :( Lactobacillus plantarum and Lactobacillus paracasei). Both species of lactic cultures can use beet juice for the producing of lactic acid and the synthesis of cells.Theprocess of fermentation was carried at 30°C for 24 hr. by inoculated of beet juice using lactic cultures. Lactobacillus plantarum and Lactobacillus paracasei grew well on the vegetable juice of ((Beet plant)) and reached nearly (12.5± 4.12×10) and (8.6± 5.20×10) CFU/ml respectively followingfermentation for 24 h at 30°C. After cold storage for 6 weeks at 4°C, PH, Acidity as (Lactic acid), viable cell counts were detected weekly.As a result the viability of L. plantarum and L. Paracasei were increased (9.03-9.69 log CFU / ml) respectively on the 42 day of cold storage while, PH of these bacteria lowered to (2.1± 0.020) respectively therefore, increased acidity of fermented beet juice whilst the viability of cell counts of L. plantarum and L. paracasei were not lost during cold storage at 4°Cconditions.
109 Survey of the Bacterial Contamination in the Environment of the Operating Theaters over Two Years’ Time in a Multi-specialty AL-Yarmouk Teaching Hospital in Baghdad/Iraq, Ali H Hayyawi, Sarab K. AL-Rawi,Manar S Assi, Areej M. Mohammed, Rakeaa M Al-Mulimawi, Yahya A. Fouad, Ban N.Lateef
Background: The hospital environment, the design of buildings especially operating theaters and associated facilities play an important role in the spread of nosocomial infections and surgical site infection. Methods: A descriptive study. It covers 30 months; Weekly swabs were collected from thirty-two operating theaters. Results: The highest rate of contamination was in the general surgical OT with 12.9%, followed by Orthopedic OT (10.7%). On the other hand, Ophthalmic OT recorded the least bacterial contamination with (3.1%) only.During the three years’ time of the study; we saw heavy growth of G-ve bacteria:P.aurogenosa195(35.8%), 137(43.4%) and 48(53.3%), thenE. coli141(25.9%), 115(%) and 28(%) respectively. Conclusion: Contamination was identified in all OT in different branches. Bacterial growth reported throughout the year and in different months. Despite the availability and usage of the sterilization materials, contamination still there.
110 The Role of Adding Sodium Chloride in Broiler Chicks Diets to Improve Production Performance and Antioxidant Status during Heat Stress, Al-Enzy AFM, Zaeid JM Saed, Ahmed S Naser, Th T Mohammed, SM Abdulateef, FMH Al-Khalani, FM Abdulateef
This study was conducted at the Poultry Research Station in the Livestock Research Department / Agricultural Research office / Ministry of Agriculture, where a 84 sexed chicks that brought from a local hatchery ( AL-Shuker) in Abu Ghraib were used at one day old with initial weight of 40g of the breed (Ross 308).The continuous light method (24 hours/day) and the gas incubators were used to warm the hall in order to reach the requiredtemperatureof38℃until the end of the experiment at 42 days. The chicks were randomly distributed on 2 treatments, where each treatment included three replicates of (14 chicks /rep), the first one was the control treatment, and in the second treatment the chicks were given sodium chloride by 250 mg/kg feed. The study findings showed a significant superiority (P
111 TUSC3 Expression in Childhood Acute LymphoblasticLeukemia Patients in Baghdad, Iraq, Zaid Khalid, Fadhel M Lafta, Abdul-Ameer N Ghaloub Al-Rekabi
Acute lymphoblasticleukemia (ALL) is the most common childhood cancer world-wide. This disease is characterized by wide-range of recurrent genetic aberrations that have an impact on the disease initiation and patient’s outcome. This study was set to investigate the expression of TUSC3, tumor suppressor candidate 3, in a set of 31 cases diagnosed with ALL at the Child’s Central Teaching Hospital, Baghdad- Iraq using qPCR technique. Down-regulation of TUSC3 was observed in the majority (17/31(64.5%) of the studied ALL cases, while the rest of the patients (14/31(35.5%) have shown normal to unregulatedTUSC3 expressionsuggesting a key role for this gene in the diseases biology.The expression level of TUSC3 gene could be investigated further, in large scale studies, for its diagnosis and prognosis value, especially within the different cytogenetic subtypes of ALL.
112 SOME FACTORS AFFECTING PREGNANCY RATE DURING ARTIFICIAL INSEMINATION IN CATTLE, Haidar R Abbas, Hussein A Khamees, Jawad K Taher
This study was conducted in the northern regions of Basra province / Iraq from March 2017 to February 2018. Nine-hundred cow age ranges between 2 to 5 years, weights from 200 to 400 kg and have crossbreeding and local breeds.Prior to initiating the artificial insemination, the rectal palpation of the cows was performed to determine the estrous phase and palpation the ovaries to determine the ovarian state and ovulation site. The cows was inseminated artificially using three different sources of frozen semen (Holland, Iranian and Iraqi) source with two different straw volume (0.5 or 0.25) ml., and during the insemination; the deposition of semen was performed in three different sites of female genitalia (uterine horn, uterine body and cervix).Depending on the source or origin of the frozen semen, the results showed there are no effect on successful insemination when use of frozen semen with a Holland, Iraqi or Iranian source, there are no significant differences at p < .05. (F = 1.1877).Depending on the size of the straw used (0.25 ml or 0.5 ml); the results showed there are no significant differences between the two volumes at (p < .05).Depending on the site of semen deposition within the female reproductive system; the results showed there are a significant differences at (p < .05) and (F = 8.73035). The highest percentage of successful inseminations showed when the deposition of semen in the uterine horn, compared to the deposition in the body of the uterus or cervix. Depending on the number of inseminations, the results showed that there are no significant differences between the study groups at (p < .05) and (F = 60.54751).The total percentages of successful inseminations in this study were 785/900 (87%), while the failed inseminations were 115/900 (12%).After third insemination; the cases were excluded and considered as a Repeated Breeders cows.
113 Using the ARIMAModels to Predict Wheat Crop Production in Iraq , Najla Salah Madlul1, Eman Younis Al –Najjar, Yusra Tariq Baker, ZawidFathyAbd Al-Ramthany
The question of prediction has gained considerable interest in the economic studies, whereby decision makers can draw economic and social policies for the coming periods depending on available data about the history of the studied phenomenon. Many ways of economic prediction have emerged, the most prominent of which are the Autoregressive Integrated Moving Averages (ARIMA). This method depends on merging Autoregressive model and the moving averages. The aim of research is to employ the (ARIMA) modelfor predicting the crop of wheat in Iraq, for the high accuracy of these models in analysing time series and prediction. The annual data have been used to produce the crop of wheat for the period between (1988-2018). The results show that model (1.0.1) (ARIMA) is the most convenient one to prediction the evolution of wheat production in Iraq till 2028 according to the statistical tests of the accuracy of prediction models. Results show that the production of wheat has increased in Iraq according to the suggested model during the ten coming years, with an annual average higher than that of the study period (1988-2018), whereby the annual growth average of production reached (0.94%).
114 Extent of microbiological contamination among swabs drawn from selected wards and kitchens at AL -Yarmouk Teaching Hospital in Baghdad –Iraq, SarabK Alrawi, Areej M Mohammed, Manar S Assi, Ali H Hayyawi, Ban N Lateef, Yahya A Fouad
Objective: To evaluate the level of contamination in different hospital’s wards and kitchens in AL-Yarmouk teaching hospital in Baghdad -Iraq. Method: The study was done from the 1st of January, 2017 to the 30th of June, 2019. A total of 7742sterile cotton swabs were collected randomly from the following wards: Burns, RCU, CCU, NICU, (Dialysis, Bronchoscopy, Endoscopy) unites and Kitchens. Samples were cultured in different agars and incubated for 24-48 hrs at 25 °C and 37 °C for fungi and bacterial growth, respectively. Results: Out of 7742 sample, 1227 swabs were reported positive. By 2017, Gram-positive bacteria were more prevalent with 59.5%, and Bacillus was the most common isolated .The rates of Gram-negative bacteria became more predominant with 54.9% and 69.4% in 2018 and 2019, respectively and E. coli was the main isolated bacteria with prevalence rates of 32.1% in (2018) and 42.4% in (2019). The most contaminated wards were CCU (36.6%) in 2017, RCU (32.8%) in 2018, and NICU (10.6%) in 2019, while Peritoneal dialysis showed a continuous increase of contamination from (3%) in 2017 to (9.8 %) in 2019. Conclusion: Gram- negative bacteria prevalence is increasing. Taking measures to decrease the contamination is essential by following the guidelines and the recommended practices.
115 A study of alexithymia in patients with peptic ulcer diseases, Adil J Abdul Sahib, Kasim O Hussein, Dergham M Hameed, Emad Salih, Haneen Kareem
One hundred subjects [50 PUD (24 women, 26men)],and [50 healthy individuals (22 women, 28 men)] in in Al-Hussain teaching hospital in Samawa city were evaluated.. The two groups were matched with regard to demographic variables. Alexithymia was evaluated by appropriate questionnaire. Alexithymia symptoms were higher in PUD patients than healthy individuals (difficulty in identifying feelings 16.4±4.8 vs 13.10±3.24, difficulty in describing feeling 24.28±6.91 vs 19.06±5.38, externally oriented thinking 25.68 ±6.87 vs 17.36 ±3.85). The results show that PUD patients experienced more alexithymia than the healthy subjects, so we think that such patients should be evaluated also in psychiatric department.
116 Isolation of yeasts from Otomycosis cases in children and dogs, Zainab AA Al-haddad
Otomycosis usually unilateral with scaling, itching, and pain as the primary symptoms. The infection is either subacute or acute. This research was conducted to isolate yeasts from otomycosis in children and dogs in Baghdad city. Ear swabs from( 100) child which were diagnosed clinically in central educational hospital of pediatrics and( 100) dog's cases were brought to private clinics with otitis symptoms ,and subjected to fungal isolation by macroscopic and microscopic methods by using RapID Yeast Plus System for yeasts identification. The results for yeasts isolation from ear swabs of children suffered from ear infections were thirty four (34)yeasts isolates (34%), in which C. albicans appeared as highly occurrence in ten (10)isolates(10%) whereas Cr. albidus showed nine (9)isolates(9%), C. tropicalis with eight (8)isolates (8%), C. lusitaniae with three (3) isolates (3%) as well as C. krusei and C. intermedia appeared with two (2) isolates (2%) for each one of them ,while the results of yeasts isolation from dogs ear swabs suffered from problems in ears were sixteen(16) yeasts isolates(16%), C. albicans represent highly appeared species with five (5) isolates (5%) while C.glabrata appeared with three (3) isolates (3%) whereas Cr. albidus and R.rubra showed four (4) isolate (4%) for each of them.
117 Pharmacomodulation of positions two & four of nucleus five-nitroimidazole by using palladium catalyze, Noor Thamer Abbas Al Saadi, Marwah Thamer Abbas Al Saadi, Zina Tahsin ALi
Cross-coupling reactions consist of reactions between an electrophilic organic species such as a halide or a pseudohalide (triflate, tosylate, mesylate) (R1-X) and an organometallic reagent (R2-M; M = Mg, Li, Cu, Zn, Al, B and Si). These reactions are catalyzed by a metal transition complex based on nickel or palladium, and result in the formation of new CClO.sub.2 bonds. Many cross-coupling reactions have thus been developed in about twenty years, by varying the nature of the electrophile, organometallic reagent and transition metal (Scheme 1). The most commonly used coupling reactions involve palladium as a transition metal, and the diversity of these reactions is based on the type of organometallic used, based on aluminum, zinc or zirconium (Negishi coupling), [1] boron (Suzuki coupling), [2] magnesium (Kumada coupling), [3] tin (Stille coupling) , [4] or silicon (coupling of Hiyama) .[5] The development of this type of reaction allowed Richard Heck, Ei-ichi Negishi and Akira Suzuki to be awarded the Nobel Prize for Chemistry in 2010.[1].
118 Comparing the effect of some plant extracts and commercial washes against some isolated Microorganisms from vagina of pregnant women, Angham J Alwan, Sabreen J Alwan
The main role of the present study is to isolate and diagnose the main pathogens of female genital tract vaginal infections in pregnant women 3 rd trimester months, as well as the inhibitory effect of commercial vaginal washes and the effect of a group of plant extracts on these bacteria to evaluate the possibility of using these extracts as alternatives to commercial or other treatments. Thirty (30) vaginal samples High Vaginal Swab (HVS) were collected from pregnant women with different ages after the internal sonar trans vaginal U\S to women with clinical symptoms associated with vaginal and cervical infection. These samples were tested to achieve the objectives of this study. The results of isolation and diagnosis showed the presence of (17) positive samples (56.66%) of bacterial culture belonging to Staphylococcus aureus bacteria. Four samples of Staphylococcus epidermidis (13.33%) while E. coli was detected in two samples (6.66%). The study showed the prevalence of Candida either alone in (6) samples (20%) or in combination with the bacteria in (14) samples (46.66%). By evaluating inhibitory activity of the commercial vaginal wash (Germex), a significant effect was found on the studied bacteria when compared with a number of water extracts .The water extract of honey had the most inhibitory effect on the bacteria followed by the extract of the apple vinegar, while olive oil and Chamomile (Matricaria chamomilla) showed a variation according to the type of bacteria and their resistance.
119 Effect of Radiation Dose Reduction on Parameters in Pediatric Head CT Examination, Bashar Adnan Munshid, Ammar Alhassan, Zina Tahsin Ali
Radiation must be as low as can be reasonably achieved for CT examination, particularly in pediatric patients. This study was conducted by using two procedures (control and modified) prepared with 64-slice Siemens CT scan (SAMTOM Definition), calibrated in accordance with international protocols. A total of 60 pediatric patients underwent brain examination for a variety of clinical diseases. The effective dose (Eff.dose) was calculated and effective current-time products (in effective milliampere-seconds, Eff.mAs) were measured. Calculation of Eff.mAs and Eff.dose was performed for each CT examination; these were statistically analyzed by using one-way ANOVA and independent t-tests, in accordance with the (ICRP) report for estimating risk (K) factor. Two procedures of standard (control) protocol and modified head CT scans were reviewed for the diagnosis of pediatric patients, 0-12 years of age. There were no gender-based differences between these two procedures with respect to either Eff.dose or Eff.mAs. However, there was a strong relationship between patient’s age and both (Eff.dose and, Eff.mAs), indicated by the significant P value (p
120 BUILDING THE PREDICTIVE SCALE FOR THE PSYCHOLOGICAL EFFICIENCY OF THE ATHLETES STUDENTS AT AL-FURAT AL-AWSAT TECHNICAL UNIVERSITY, Hameed Shamkhi Ghazi
The objectives of the research focused on the preparation of a questionnaire to build a predictive measure of psychological efficiency for the students of the athletes of the University of the Middle Euphrates technical, as well as "the recognition of predictions of psychological efficiency, and applied research on a group of athletes at the University of the Euphrates Middle Tech for the period of 22/11/2017 and the researcher used the descriptive method in the survey method. The most important steps of the research are to limit the number of athletes in Al-Furat Al-Awsat Technical University from the students. The main questionnaire was composed of (48) Building a collective and individual scale, as required (325) questionnaires were returned (301), (24) of which were excluded due to lack of complete answer. The number of valid questionnaires reached (265) questionnaires In the final statistical analysis, using the appropriate statistical methods, and then presenting them, analyzing them and discussing them in a scientific manner based on the relevant sources and references, and then concluding the conclusions, the most important of which was the establishment of a suitable questionnaire to build a predictive measure for the psychological efficiency of the students. In the light of these conclusions, the researcher recommended a number of recommendations, including the possibility of using the current measure by the supervisors of athletes at the University of the Euphrates Middle Tech, as well as the need to pay attention to the psychological side, especially with regard to students in the same physical, skill and educational aspects.
121 IN VITRO CYTOTOXICITY EVALUATION OF NOVEL COMBINED PLANT CRUDE AQUEOUS EXTRACT OF LICORICE AND MACA, Aveen R Mohsin, Saad S Al-Dujaily, Muktar K Haba
Recently, researchers have shown an increased interest in the potential positive effects of Glycyrrhiza glabra (licorice) and Maca extracts. However, there has been no detailed investigation of the effects of mixing these two plants extracts. Therefore, the present research explores for the first time, the effects of the mixture of these two extracts. The aim of the current study is to figure out the cytotoxicity of this mixture on the sensitive system of the mammal's body. This study, therefore, set out to assess the cytotoxicity effect of the aqueous crude extract of Licorice, Maca, and the effect of their extracts mixture on mice normal spleenocytes by MTT assay. The results show that the cells growth inhibition of both extracts and their mixture was 0% compare to control. The mixture medium (12.5/2.5mg) has the best viability with 245.0% compared to Licorice medium alone (12.5mg) with viability 223.1% and Maca medium alone (2.5mg) with viability 133.6 %. The most obvious finding to emerge from this study is that the mixtures of licorice and Maca extracts have no harmful effects at a dose of (12.5/2.5mg). Furthermore, the mixture data can be applied for orally used in the treatment of infertility status.
122 RULES OF RHOTON AND SACCULAR INTRACRANIAL ANEURYSMS: AN IN VIVO RADIOLOGICAL-ANATOMICAL CONFIRMATORY STUDY IN IRAQI POPULATION , Ali Adnan Dolachee, Ghazwan Alwan Lafta, Alyaa Khalid Al-Zubaidi, Samer S Hoz
Intracranial aneurysms are a weakened arterial wall due to loss of internal elastic lamina resulting in dilations of the intracranial arteries and these aneurysms are susceptible to rupture, resulting in life-threatening hemorrhage; therefore represents chief cause of hemorrhagic stroke. Rhoton rules are used for the planning the operative approach to these aneurysms. Aim of the study: to evaluate the application of Rhoton rules in our patients with intracranial aneurysms. A retrospective series of 283 aneurysms, from 1st of January 2016 to 1st of December 2018 in Neurosurgical Teaching Hospital Baghdad/Iraq, diagnosed by CT angiography with 3D reconstruction were included in this study to evaluate the age, sex, site, size, locations and application of Rhoton rules in these aneurysms. Of the 283 patients studied, we found 127 aneurysms at the anterior communicating artery (location 1), 80 at the posterior communicating artery (location 2), 37 at the bifurcation of the internal carotid artery (location 3), 21 at the middle cerebral artery (location 4), and 18 in the posterior circulation (location 5). The four rules of Rhoton about intracranial aneurysms: rule one (100%), rule two (84.5%), rule three (84.8%) & rule four (84.8%). The rules of Rhoton is a heterogeneous concept which summarizes several hemodynamic principles and is verified in 80% of aneurysms in Iraqi patients, with different frequencies depending on the rule and location. These findings should be verified in other populations, as our patients are of Hispanic origin in 99.7% of the cases.
123 SELF-INFLICTED BURN AS A METHOD FOR SUICIDE AT AL- DEWANYEA CITY, IS IT A COMMON METHOD AND WHAT ARE THE CHARACTERISTICS OF THE VICTIMS?, Saher A Albermany
Suicide is a very stigmatising condition in Iraq and perceived stigma by the public make recorded data about the subject far from accurate. So researching this interesting phenomenon remains a very important step in evaluating its impact on society in general. The aims of the study were. To compare between numbers of victims of self-inflicted burn with the official police suicide statistics that is recorded each month in the city and to identify the socio-demographic characteristics of the patients in addition to their past psychiatric history.During the year 2018 any patient admitted to the burn centre at al Dewanyea with a suspicion that his/her burn was self-inflicted was asked permission of interview by the researcher. The patients answered questions regarding their socio-demographic profile and motives and were asked if they had previous history of any psychiatric condition.A total of 34 patients admitted of burning themselves during that year while the official police records showed only 6 patients killed themselves using this method in the same time period. More than 88% of the patients had no previous attempt, only 6 patients were known psychiatric patients and in all the cases there was no plan. Half of the sample was of the 20-40 years' age group, females were slightly over represented (about 55% of the sample) and most of the males were single. About 55% of the sample were un employed and 44% of them were illiterate. Only 2 patients survived the burns eventually. Family problems were the most common precipitative factor (in more than 91% of the cases). There is poor registration of suicide numbers using self-burning at al Dewanyea city. Most of the cases had no previous attempts and had no history of mental illness and in all the cases the act was impulsive with no previous plan.Those who burn themselves are most commonly young, illiterate and unemployed.Men were mostly single while women were more commonly married. There was high mortality rate in the sample.
124 PREVALENCE OF FUNGAL PATHOGENS AMONG RESPIRATORY TRACT INFECTIONS, Abbas M Al-Ammari, Anaam Fuad Hussain, Thuraya Kadhim Ismael
One-third of infections associated mortality is caused by respiratory diseases. Among these accounting, fungal respiratory infections are largely unrecognized. Although treatment, most invasive fungal infections stilled responsible forhigh mortality. Fungal spores typically enter the lung by inhalation, the spores reach lung through the bloodstream and cause fungal pneumonia. Inside the alveoli, the fungal spores travel throughout intracellular spaces and also passage to adjacent alveoli through connecting pores. One hundred twenty specimens including bronchoalveolar lavage, sinus, and sputum (40 specimens each) were randomized collected from patients with respiratory problems who attended Baquba Teaching Hospital for a period 7 months.Fungal cultures were performed using primary culture onto Sabouraud´s dextrose agar containing chloramphenicol which incubated at 28ºC for 1-2 weeks. The positive results were identified using standard conventional methods. Candida species reportedalmost fungal pneumonia isolated from bronchoalveolar lavage and sinus sites, with a percentage 28.6% and 26.7%, respectively. Whilst, Aspergillus fumigates was the almost fungal isolates from sputum specimens, with a percentage 24%. Young and adult groups were most percentage infected with Candida species (35.7% and 21.1%), respectively. Whilst old aged were most infected with A. fumigates (23.8%) The findings concluded that Candida species and Aspergillusfumigates reported the highest frequency of fungal pneumonia infections.
125 ISOLATION AND GENOTYPING OF Vibrio cholerae ISOLATES FROM PATIENTS WITH CHOLERA DISEASE IN BABYLON PROVINCE, Hussein T Abdulabbas, Ilham Abbas Bunyan,  Lamees A. Abdul-Lateef
A total of 35 stool samples were collected in the Central Health Laboratory of Babylon Province from patients presenting invasive cholera disease from November 2017 to December 2018. The results revealed that all suspected stool samples showed positive isolation (100%) to Vibrio cholera species. Regarding serogroups diagnosis, the results of this study showed that O1 serogroups were the predominant serogroup among all clinical samples with high rate 94.3% (N=33), while only two isolates of non-O1/non-O139 (NAG) (5.7%) were documented as a causative agent to cholera or cholera-like disease. These results were confirmed by new optimized Touchdown-Multiplex PCR assay depended on ompW, O1 and O139-specific primers. Meanwhile, the results of the study showed negative result to identify O139. With regard to biotypes diagnosis, the results of this study indicated that El Tor biotype had prevailed in all clinical isolates of V. cholerae with a high rate (100%) compared with the Classic biotype. These results were confirmed by new optimized multiplex PCR assay designed with ompW, Classic and El Tor-specific primers. In identification of serotypes, Inaba serotype had prevailed in all V. cholerae O1 isolates with a high percentage 97% (N=32), while only one Ogawa serotype (3%) was reported as a causative agent for cholera disease in Babylon province. A new quadruplex PCR assay was optimized to identify toxin-related genes (ctxA, zot, rtxAand stn/ato) simultaneously. The results revealed that all studied genes (ctxA, zot, rtxAandstn/sto) were detected in V. cholerae Ogawa serotype (100% positive) while only ctxA, zot, rtxA genes were identified in Inaba serotype with 100% positivity. Moreover, only rtxAandstn/sto genes with 100% positivity were identified in V. cholerae (NAG) strain.
126 MOLECULAR STUDY OF CDK1 SNPS IN IRAQI PATIENTS WITH COLORECTAL CANCER, Shaiman A Al-Oubaidy, Ali H Al-Saado
This study includes (100) samples which grouped as following: control group (35) sample (blood and benign), patients group including (30) blood samples and (35) FFPE samples (tissue imbed in paraffin). The age of study samples were ranged from 30-89 years old men and women). These samples were grouped in six aging groups that each 10 years in one group. results indicate that the age risk of disease incidence is begin in 50 years old and increased between 60-69 years old for both sexes. The CDK1 gene was detected by PCR-sequencing in order to investigate if there were any genetic defect within this gene. The alignment results of the 254 bp samples revealed the presence ofSNP (rs2456777) that distributed variably in the analyzed samples in comparison with the referring reference DNA sequences. The results showed that the allele G is responsible for the appearance of disease and its progression in patientsby reaching to metastasis stage and have gene expression by tumor molecular phenotype, with significant value in tissue samples.
127 PREVALENCE OF Β -LACTAMASES ENZYMES AMONG ENTEROBACTERIACEAE IN DIFFERENT IRAQI PROVINCES: A REVIEW , Abeer Mohammed Ali Al-Garawyi
Gram negative bacteria are responsible for most infections among people around the world. The level of antibacterial resistance in gram negative bacteria in Iraqi provinces, specifically, Baghdad, Hilla and Karbala, has not been previously studied. This study shows a high resistance rate among the governorates due to gram negative bacteria producing ESBLs and carbapenemase enzymes, these infections are mostly opportunistic. The recognized β -lactamase genes are CTX-M, OXA-10, NDM-1, TEM, SHV,IMP-1 and VIM-2 have found in many isolates.But, less common β -lactamase genes are PER β -lactamase in Hilla city. So asnumerous possible factors unique to the Iraqi provinces may have responsible of appearance β -lactamases, comprisingthe excessive consumption of antimicrobial agents and a large proportion of travelers are mainly from the Iranian, Pakistan and Indian countries.
128 The effectiveness of using r-HMG+r-FSH vs. r-FSH alone during COS on ICSI outcome, Saba Sabeeh Hussain, Muhjah Falah Hassan
Controlled ovarian stimulation (COS) has been used during an assisted reproduction cycle to improve the success rate. The gonadotropins which are most frequently used during COS/ICSI cycles are r-FSH and r-HMG (FSH+LH). Over many years, outcomes achieved with using both gonadotropins during COS have been compared and the superiority of using both or only r-FSH is still a controversial topic that necessitates further studying. The aim of this research is to study the effectiveness of adding r-HMG to r-FSH on ICSI outcome in infertile patients who underwent COS. Fifty infertile couples were included who attended fertility clinic due to either female factor or male factor infertility and all of them were treated by ICSI and subjected to COS by r-FSH with or without r-HMG. ICSI outcome was assessed in the form of the total number of retrieved oocytes, FR, CR, embryo quality and chemical PR.The study showed that there was no significant difference between using both gonadotropins or FSH alone regarding the total number of oocytes produced, fertilization rate (FR), cleavage rate (CR), embryos number and quality except pregnancy rate (PR) which was lower with adding r-HMG 41.66% VS 50 % with r-FSH alone. We concluded that the combined use of r-HMG+r-FSH is effective as r-FSH alone in terms of oocytes' and embryos' quality while the effect on PR is still undetermined.
129 Evaluation of pneumothorax in the first month of life, Omar Abdulqadir Ajaji, Bassam Maddah H Al=Alosi, Raad Hameed Khalaf
Pneumothorax is relatively more frequent in the first month of life than in other times of life. We aim to analyze the demographics, characteristics, incidence, factors associated, seasonality, treatment modality and mortality of pneumothorax of newborns. The methods that we used were a cross-sectional study of 97 newborns with pneumothorax over a period of 24 months January 2017 and December 2018. The patient’s demography and characteristics of delivery were studied. Additional analyses of the incidence, severity, risk factors associated it, monthly and seasonal variation, diagnostic workup, treatment modality and mortality of pneumothorax. Our results show that the pneumothorax was diagnosed in 97newborns with an incidence of 7.1%. 57 were males and 40 were females. 43% were preterm and 57% was the term. The birth weight was normal in 60 newborns. 64% were born inside the hospital. 61% were delivered by cesarean section. Of these, 57% right side, 40% left side and 3% bilateral. Pneumothorax was mild in 11%, 48% moderate and 41% tension. A slight spring and summer preponderance, 19(20%) ,19(20%) occurred in spring (2017,2018 )respectively and 17(18%),18(19%) in summer (2017,2018)respectively . Active resuscitation was the predominant associated factor that occurred in 39%. 97% of pneumothorax required drainage. 21% of newborns died. The highest mortality was observed in severe pneumothorax. We concluded that the incidence of newborns pneumothorax was relatively frequent. Newborns resuscitation should be done very carefully. More studies are needed to investigate the seasonal factors of pneumothorax in the 1st month of life.
130 Prime boost HIV vaccination with recombinant influenza virus vectors stimulates specific and mucosal CD8+ T cell immune response in BALB/c mice, Mohammed Jasim Mohammed Shalla
HIV/AIDS continues to be a significant medical problem worldwide. An effective and safe vaccine remains a high priority. Most HIV vaccine candidates to date have failed to elicit effective immune responses that are necessary to control HIV infection. The results of a promising phase III trial conducted in Thailand using a recombinant canarypox vector vaccine (ALVAC) expressing HIV Gag in combination with recombinant HIV-Env glycoprotein gp120 (AIDSVAX), showed 31.2% efficacy in humans and raised the prospect of a protective vaccine. The most recommended HIV vaccines are focusing on inducing specific CD8+ T as a critical immune response to control progression and dissemination of HIV virus from the site of infection into different mucosal compartments of the body.This study project used influenza viruses as a mucosal live vaccine vector to stimulate effective CD8+ T cell immunity. Recombinant influenza A viruses, H3N2 (HK-X31) and H1N1 (A/PR8/8/34) expressing defined mouse HIV-1 CD8+ T cell epitopes (H-2Kd Gag197-205 and H-2Kd Tat17-25) in the neuraminidase (NA) stalk were generated using reverse genetics and administered as a prime-boost vaccine within various mucosal routes of vaccination, intranasal-intranasal, intravaginal-intravaginal, intranasal-intravaginal and intravaginal-intranasal vaccination in BALB/C mice. Following those prime-boost vaccinations, tetramer and intracellular cytokine staining assays used for the detection of specific CD8+ T cell immune response in harvested organs, spleen, bronchoalveolar lavage (BAL), mediastinal and inguinal lymph nodes. In addition, mucosal HIV-specific CD8+ T cells were detected using specific anti-mouse CD8α antibodies directed against specific integrins (LPAM-1 and CD103). Moreover, the level of specific cytokines, such as interleukin-15 (IL-15) detected within specific mucosal CD8+ T cells for the detection of the migrated HIV-1 Gag+ CD8+ T cells.Our result showed there was an induction of CD8+ T cells targeted H-2KdGag197-205, compared to no CD8+ T cell responses specific for H-2Kd Tat17-25 in recombined influenza-HIV vaccinated BALB/c mice. Also, comparable HIV and endogenous influenza-specific CD8+ T cell responses following intranasally-intranasally prime-boost vaccination in harvested lymphoid tissues, spleen, bronchoalveolar lavage, and mediastinal lymph nodes compared to inguinal lymph nodes which included a high proportion of specific CD8+ T cell immune response following intravaginal-intravaginal prime-boost infection. Moreover, a proportion of these cells isolated from mice infected with recombinant influenza-HIV vaccine intranasally-intranasally prime-boost expressed mucosal surface integrins, especially LPAM-1(α4β7) of local and distal lymph nodes higher than the levels observed following intravaginal vaccination. In addition, mucosal LPAM-1+HIV-Gag197-205+ CD8+ T cells harvested of intranasal prime-boost vaccinated mice were recognized by a high expression of IL-15 compared to LPAM-1-HIVGag197-205+ CD8+ T cells.We conclude that the intranasal prime-boost vaccination as one of the mucosal routes of vaccination using recombinant influenza viruses as mucosal viral vectors of HIV vaccine in BALB/c mice has an important role in stimulating both specific and mucosal CD8+ T cells within a high level and these cells would be important for migration of mucosal specific CD8+ T cells given the mucosal acquisition of HIV infection and control of HIV-1 virus dissemination through mucosal compartments.
131 Estimate the level of cholesterol and triglycerides in patient with type II diabetes in the province of Najaf , Ahmed Adnan Al-Khafagi, Manar Kareem Alquraishy, Alia Hussein Al-Mousawi
Background and Objectives: Diabetes mellitus (DM) is a group of metabolic disorders of carbohydrate metabolism in which glucose is underutilized, producing hyperglycemia. Furthermore, it is proposed that the underutilization of glucose is associated with changes in the lipid profile. Changes in lipid profiles are also well related to the severity of DM. This study involved 50 were patients admitted with a diagnosis of DM; blood samples were drawn under aseptic precautions from cases of DM and healthy controls. Necessary investigations were carried out and values were tabulated for cases and controls separately for statistical evaluation. Our Results show that the DM patients compared to controls significant increase in the following parameters were observed. Cholesterol ratios increased. Furthermore, the levels of triacylglycerol increased. We conclude that there is a statistically significant large effect in cholesterol and TG a level of cases compared with controls, cholesterol level is increased and TG levels were well associated with blood sugar also increased.
132 The differences between benign mixed tumor and papillary cystadenoma lymphomatosum in proliferative, apoptotic, and antiapoptotic activity, Ameera Kamal Khaleel
The majority of salivary glands tumors are benign mixed tumors. The present study was aimed to evaluate the differences between benign mixed salivary glands tumor and papillary cystadenoma lymphomatous regarding the tumor cell proliferation rate, apoptotic and antiapoptotic activity. The study was performed on archived paraffin-embedded salivary glands tissue of 23 benign mixed tumors and five cystadenoma lymphomatosis. Sections were stained with hematoxylin and eosin and cases with the definite diagnosis were selected for immunohistochemistry. The immunoreactivity was assessed in areas of highest positivity regarding Ki-67, P53, and bcl-2. Our results refer that benign mixed tumor and papillary cystadenoma lymphomatous showed negative immune expression for Ki-67. The P53 immune staining in benign mixed tumor appeared negative, while the papillary cystadenoma lymphomatous appeared with mild positive staining. Bcl-2 immune expression in the benign mixed tumor was moderate positive while the papillary cystadenoma lymphomatous was mild positive. We conclude that the benign mixed tumors showed significantly more Ki-67 and bcl-2 immune labeling indexes than papillary cystadenoma lymphomatous, but the P53 immune expression was less (p
133 MORPHOLOGICAL AND GENOTYPIC IDENTIFICATION OF BOVINE-INFESTING TICKS IN AL-DIWANIYAH PROVINCE, IRAQ, Nuha Qasim Mohammed, Ahmed Jasim Neamah, Monyer Abdul Amier Abd Al-Fatlawi, Hala Mohsin Mutter, Hassan Hachim Naser, Israa Najm Abdullah Al-Ibadi
Tick skin invasion is one of the important ectoparasitic infestations occurred worldwide and in Iraq, which is correlated with tick-borne diseases such as theileriosis, babesiosis, and anaplasmosis; however, more recently, in Iraq, an identified number of cases belonged to Crimean-Congo hemorrhagic fever (CCHF) were caused and transmitted via ticks that infested ruminants in Al-Diwaniyah Province. According to this importance, the present characterizing study was launched to identify the current circulating ticks in this province. Inspection of 196 bovine animals (males and females distributed over different regions and seasons) for the presence of ticks; 54 animals were found to have such parasitic infestation. From those infested animals, 108 adult ticks were collected. Ticks were exposed to a series of morphological identification in the Department of Microbiology and Parasitology, College of Veterinary Medicine, University of Al-Qadisiyah and the Natural History Museum, University of Baghdad. Moreover, genotypic characterization was performed using a polymerase chain reaction (PCR) method targeting the 18S rRNA gene of 50 ticks. Eight positive PCR products were sent out to sequencing targeting the same gene,thenphylogenetic analysis was conducted to understand the evolution of the ticks. Morphological characterization revealedthat the ticks were from genera; Hyalomma and Rhipicephalus. Significant (p˂0.05) differences were detected via the seasonal distribution of tick infestation with the highest rates during summer. No significant (p˃0.05) differences were unveiled via the gender or regional distribution of the tick infestation. PCR and the sequencing have confirmed the morphological-based identification,Phylogenetic study findings revealed that the Hyalomma genotypes from the present work were in a high nucleotide sequence matching with a strain of Hyalommatruncatum (KY457496.1) from South Africa and phylogenetic analysis revealed that the Rhipicephalus genotypes from the current study were closely related to a Rhipicephalusturanicus strain sequence (KF958452.1) submitted to The Hebrew University of Jerusalem, Israel. The investigation, here, provides confirming data about the presence of the Hyalomma and Rhipicephalus induced infestation in the current city which might give extra evidence about the presence of the CCHF as those ticks, especially Hyalomma, considered as major vectors for the transmission of the virus.
134 EPSTEIN-BARR VIRUS INFECTION IN THALASSEMIA PATIENTS RELATED TO BLOOD GROUP IN MOSUL / IRAQ , Anmar Ahmed Al Taie, Momammed Abdulrazzaq Ibraheem, Khansaa Basem Fadhil
Epstein-Barr virus (EBV) belongs to the family of herpes viruses and about 90% of adults worldwide have antibodies against it. EBV infections are widespread in multiple patients with blood transfusion, such as ß-thalassemia patients, Hodgkin's lymphoma, Burkitt's lymphoma, and nasopharyngeal carcinoma. Blood samples from (70) thalassemic patients and (20) healthy controls were subjected for the detection of the blood groups (ABO) and EBV IgM antibodies by ELISA assay. The types of EBV infection as acute, late or reactivated were distinguished using a new immunoblot technique. The presentstudyshowedthat theEBV infection wasfound in (12.33 %) of patients with thalassemia and the highest incidence was (44.44%) in a class AB blood group subjects. Depending on Immunoblot techniquethetypeofinfections was distinguished, the results showed thatthehighestpercentage recorded was(55.55 %) in acuteinfections, followedby (22.22%) at late and reactivated infections. The present study found that thalassemia patients in Mosul / Iraq had a correlation between EBV and blood groups AB.
135 CYTOKINES AND OXIDATIVE STRESS PROFILES IN IRAQI PATIENTS WITH VITILIGO, Yasir W Issa, Shahlaa M Salih
Vitiligo is a developed depigmentation disorder consisting of two forms which are segmental vitiligo (SV), affect one side of the body in 50% of individuals and non-segmental vitiligo (NSV) which is more common and affects both side of the body. A studydocumented that vitiligo susceptibility genes that are linked to immune regulation and immune targeting of melanocytes which are Tumor necrosis factor alpha (TNF-α), Interferon gamma (IFN-γ) and interleukin 10 (IL-10), are responsible for the pathogenesis of vitiligo. This study was designed to shed light on cytokinesand oxidative stress which may play a critical role in the pigmentary process of NSV. Fifty NSV patients were collected from dermatology department Baghdad teaching hospital, Baghdad, Iraq. And fifty healthy volunteers’ individuals enrolled in this study during the period from November 2018 to May 2019. Some parameters including the concentration of immunological guideline TNF-α, IFN-γ and IL-10, and the level of oxidative stress H2O2 in malondialdehyde (MDA) form and superoxide dismutase SOD were evaluated in sera using ELISA technique. Serum level of TNF-α and IFN-γ were significantly higher in patient as compared to control. While serum IL-10 was significantly lower in vitiligo when compared with healthy.The assayed serum level of oxidative stress MDA and superoxide dismutase was found to be increased in vitiligo. There was an imbalance between pro and anti- inflammatory cytokines. Elevated levels of MDA and SOD suggested that an oxidative stress and antioxidants could play an adjuvant role in the management of vitiligo in addition to specific therapies.
136 THE STUDY OF MALONDIALDEHYDE, URIC ACID, AND C-REACTIVE PROTEIN IN OBESE AND HEALTHY PREGNANT WOMEN IN DIFFERENT TRIMESTERS, Allaa Jamal Shhaeat, Ahmed Aboud Khalifa
Pregnancy or gestation is a complex period of human growth and development. It is a physiological process associated with the development of maternal pathologies, such as metabolic disorders (i.e. maternal obesity and hypercholesterolemia), preeclampsia and gestational diabetes mellitus. Obesity is a worldwide epidemic, it is considered a most common medical condition in women of reproductive age, during pregnancy has short term and long term adverse consequences for both mother and child, it is caused insulin resistance. There area closes relationship between the obesity, OS, antioxidants and inflammation. The aim of this study was the assessment to oxidative stress factor changes, uric acid and CRP in obese pregnant women in Misan province.The study included (60) obese pregnant women, aged (25 -35) years. The study conducted duringNovember2018to February 2019. Diagnosed parameters done using enzyme-linked immunosorbent assay (ELISA) and mind ray (BS -230). Our results reveledalmostparameters increased during pregnancy, highestincrease in the third trimesters, it is increased significantly (p ≤0.05) in comparison with the first and second trimesters. Results of current study showed clearly the following conclusion in obese pregnant women all parameters in our study elevatedduringtrimesters
137 ANTIOXIDANT AND ANTI-CANCER ACTIVITY OF CHIA SEED EXTRACT IN BREAST CANCER CELL LINE, Hayder Ayyal Mutar, Jaafar Faez Kadhim Alsadooni
Cancer is one of the deadly disease which recorded highly incidence in last decades, cancer term refers to more than 100 types of malignant tumors have different etiological and pathological features, each of these has occupied a great deal of researches interestinglike using phytochemicals medicine. The aims of present study are one such effort exploring the beneficial role of Salvia hispanica seeds against the growth of breast cancer cells. Different extracts of Chia seeds (Salvia hispanica) were prepared, then tests were carried out for phytochemical analysis of the aqueous, alcoholic extracts also chloroform, petroleum ether and n-hexane solvent for plant extracts. Determination of the Free Radical and Antioxidant scavenging activities of the Chia seed (Salvia hispanica) was performed. Analysis of MTT Assay using Breast Cancer cell line (Mcf-7) methanolic extract show that it has many phytochemicals compound than others types of extracts also it has high percentage of free radical scavenger according to DPPH tests, the anticancer activity was carried out using methanolic extract, the results show that viability of cell line was decreased with elevation of plant extract concentrations. Therefore, this preliminary work adds on and ensures the enormous possibilities of research in this area to bring out effective drug against breast cancer for the benefit of humans.
138 Evaluation of the effect of glow plasma nitriding of commercially pure titanium dental-implant on osseo integration through mechanical and histomorphometric analysis, Hassan Jawad, Shatha Salem, Tameem Khuder
Background: Oral implantology provides a reliable and rather safe solution to replace missing teeth. An implant is defined as a biomaterial which is embedded, either incompletely or totally, into the body for restorative or prosthetic purposes. The aim of current study was to evaluate the effect of plasma nitride treatment of commercially pure titanium dental implant on bond strength at the bone-implant interface by torque removal test and histomorphometric analysis after 2 and 6 weeks in comparison to non-treated one. Methods: Commercially pure titanium plates and screws were plasma nitride treated for 10 hours using glow plasma nitride apparatus. X-ray diffraction analysis and scanning electron microscope examination were carried out on the nitride surfaces of the plates. The femur of 10 white New Zealand rabbits was chosen as implantation sites. The femur of each rabbit received 2 screws and a total of 40 screws were implanted. For each period of time, 18 screws were tested for the torque required to remove the implant from the bone and 2 screws were kept for histological examination. Results: There was a significant increase in the bond strength and the new bone formation ratio of bone-implant interface with time. The torque removal forces for plasma nitride implants after 2 and 6 weeks were 30.22, 59.56 N.cm respectively and for non-treated implants after 2 and 6 weeks were 23.61 and 56.11N.cm, respectively. The new bone formation ratio for non-treated implants after 2 and 6 weeks were 1.56 and 3.04, respectively, and for plasma nitride treated after 2 and 6 weeks were 3.36 and 4.72, respectively. Conclusion: Plasma nitride treatment of commercially pure titanium plate screws significantly increased the torque removal value and new bone formation ratio at 2 and 6 weeks compared with the non-treated implant.
139 Arlyesterase activity of Paraoxonase-1 enzyme in Iraqi patients with β-thalassemia minor, Ali W Al-Ani, Shaema S Fadel
Background: Arylesterase activity of Paraoxonase-1 (ARE-PON-1) exhibits an antioxidant role which protects lipoprotein from oxidation. It is known that ARE-PON-1 antioxidant activity associated with high density lipoprotein cholesterol (HDL-C) reduces the oxidative damage mediated by low density lipoprotein cholesterol (LDL-C). The present study was aimed to examine the level of serum ARE-PON1 in Iraqi patients with β-thalassemia minor and its relationship with lipid profile (total cholesterol (TC), HDL-C, very low density lipoprotein (VLDL-C), and LDL-C) and hematologic changes as a part of antioxidant system action. Methods: In the present study, the ARE-PON-1 activity was investigated in serum of patients with β-thalassemia minor. Results: It has been revealed that the ARE-PON-1 activity was significantly decreased (P>0.0001) in patients group compared to healthy group. This was associated with slight decrease in the lipid profile of patient group (HDL, LDL-C, very low density lipoprotein cholesterol (VLDL-C), and total cholesterol (TC), except of triglyceride (TG) which showed a significant decrease (P>0.0001) compared to healthy group). Also, hematologic changes were investigated and showed depleting in most of white blood cell (WBC) and red blood cell (RBC) components. Conclusion: Patients with β-thalassemia minor are suffering from weakness in the antioxidant defence system expressed by dropping in the ARE-PON1 level and lipid profile that stimulate oxidation of many cell particles such as HGB, promote EOS apoptosis and rise of CD risk development.
140 Micro-marsupilization for Treatment of Mucocele and Ranula, Alan Haydar, Mawlood Qassab, Shehab Ahmed Hamad Alraad
Background: Mucoceles and ranulas are common benign lesions of salivary gland origin. Different techniques had been described for their treatment; however, most of them are invasive. The aim of the present study was to evaluate the effect of micro-marsupialization in the treatment of mucoceles and ranulas. Methods: The study was conducted on 18 patients with lower lip mucocele and 8 patients with ranula of the floor of the mouth. After topical anesthesia, the lesion was punctured by 3/000 black silk suture and followed for evaluation of healing. Results: Complete resolution was noted in 83.33% (15/18) of cases of mucoceles and three recurrent lesions were surgically excised. In 80% (12/15) of the cases, the healing accomplished within one week, 13.33% (2/15) of the cases showed complete healing within two weeks and only one case (6.67%) healed within three weeks which was associated with infection. On the other hand, complete resolution of ranula lesions was noted in six cases and only two cases showed recurrences and then a complete surgical excision of the lesions with associated sublingual salivary glands was performed. In 66.67% (4/6) of cases the healing was achieved within one week, 16.67% (1/6) of cases showed complete healing within two weeks and 16.67% (1/6) of cases showed complete healing within three weeks which was associated with bleeding. Conclusion: Micro-marsupialization is simple and good noninvasive treatment option for mucoceles and ranulas and is well-tolerated by the patients with minimal recurrence rate of the lesions.
141 Clinical effects of vitamin D in patients affected with Osteoporosis in the city of Tikrit, Omar Suhil Ali Ghalib, Khulood Naji Rasheed, Abdulrazzaq  Mahmood Hassoon
Background: Osteoporosis that is characterized by low bone mass and strength which in turn increases the risk of developing fractures. It is sometimes referred to as silent disease due to its asymptomatic obsolescence until first fracture occurs. Current study was conducted to study the effect of vitamin D using a clinical dose of this vitamin given twice a day to osteoporosis patients in the city of Tikrit and from both sexes who visit Dr. Abdulrazzaq Mahmood Al-Khattabi's clinic and Salahuddin General Hospital. Methods: The research involved 86 subjects (26 males and 60 females) aged between (25-79) years to study the clinical effect of vitamin D on them. In addition, 36 subjects (20 males and 16 females) aged between (25-79 years) of healthy persons were recruited as a control group. The research subjects were divided into two groups; the first of which included the age group (25-54) years while the second included the age group (65-79) years. They were each given a dose of (5000IU) of vitamin D and a dose of (500mg) of calcium twice a day, in the morning and in the evening, for three months with follow-up of subjects periodically each month to observe the changes by measuring a number of biochemical parameters represented in the examination of bone density, vitamin D, serum calcium, serum alkaline phosphatase and C-Reactive Protein. Patients with rheumatism, taking cortisone or any item containing it, and pregnant women were excluded from this research. Results: The results shown a significant increase in bone density in both groups and for both sexes as well as higher levels of vitamin D and calcium compared to the control group. However, the results showed a significant decrease in alkaline phosphatase effectiveness in both groups. Moreover, there was a decrease in the level of C-Reactive Protein in males of both groups and in females from the first group. No significant differences were recorded in the second group compared to the control group.
142 Caution from using cardiac glycosides digoxin drug which have properties therapeutic index (Ti) narrow compare with the medications wide therapeutic index, Salah Abd-Al Kader Omran
Clinical research will be based on the practical application of randomly assigned patients over the age of 25 who numbered 730 at the Academic Teaching Hospital in Iraq - Samawa. We used drugs narrow therapeutic index (NTI), which is used with caution at a specific and calculated dose because it is life-threatening and has high toxicity. Compared to drugs that have wide therapeutic index (WTI) and less serious effects (such as aspirin, acetaminophen (paracetamol), penicillin, etc.), we selected acetaminophen (paracetamol) in patient research (a wide therapeutic index) and the dose and ratio between the toxic dose and the safe therapeutic dose of the drug, the research focuses on dilution To limit the risk of medication to patients (mortality), which is used as a measure of the relative safety of the drug in a particular drug treatment, our main work is limited to digoxin (cardiac glycosides) used in heart disease, discrimination with other drugs and knowledge, and in comparison to drugs. We used acetaminophen (paracetol) which is a broad indicator, so we based on previous knowledge in clinical pharmacy, dosage medications, therapeutic toxic dose, maximum dose, initial dose, maintenance dose, lethal dose, by knowing Therapeutic index for drug identification, therapeutic index, therapeutic ratio, TI can be calculated as a lethal dose of the drug by 50% of the population (LD50) divided by the minimum effective dose for 50% of the population (ED50), that is, TI = LD50 / ED50. This "academic" definition of TI is easier to follow in preclinical trials but opens the door to changing explanations in clinical practice. In fact, the definition of therapeutic and / or toxic effect in humans is highly dependent on the type of therapeutic or toxic effect under study, and the drug is generally considered to have a good safety appearance if its TI values exceed the value of 10. The situation is quite different from so-called narrow TI drugs (NTIDs) , Where only a very small set of doses produce a beneficial effect without causing severe and fatal complications, i.e. small differences in their plasma concentrations can lead to inadequate therapeutic response or the appearance of harmful toxic effects. The term “critical dose medication” is sometimes used to refer to drugs where relatively small differences in dosage or concentration may lead to serious therapeutic failure and / or serious drug reactions. Other terms used also include "narrow therapeutic window drugs", "narrow therapeutic range", "critical anti-dose drugs" or "narrow therapeutic ratio , According to:The US Food and Drug Administration (FDA) defines a medication product as containing NTI when (a) there is less than a double difference in LD50 and medium effective dose values (ED50) or (b) there is less than a double difference in toxic concentrations Minimum (MTC) and minimum effective concentrations (MEC) in the blood and (c) safe and effective use of the drug requires careful calibration and patient monitoring 2018.
143 THE SIGNIFICANT OF IL-2, IL-4 AND TNF-Α GENE PROMOTER POLYMORPHISM IN ASSOCIATION WITH SERUM LEVELS OF CORRESPONDING CYTOKINES IN BLADDER CANCER, Haidar A Shamran, Rana S Jawad, Fathaa A Mahmoud, Alaa I Ali
Bladder cancer (BCa) is one of the most prominent causes of cancer-related death. It is a multifactorial disease caused by a complex interaction between environmental and genetic factors. Pro- and anti-inflammatory cytokine may have a significant role in this malignancy. Polymorphisms in the promoter region of some cytokine genes can influence the gene expression of these cytokines. This case-control study aimed to dissect the impact of three single nucleotide polymorphism (SNPs) in the promoter region of IL-2, IL-4 and TNF-α genes in the susceptibility to BCa among a sample of Iraqi patients. This study enrolled 58 patients with histopathologically confirmed BCa, and other 56 age- and sex-matched, family unrelated apparently healthy individuals as a control group. Blood samples as well as demographic and clinical characteristics were collected from each subject, and DNA was extracted from whole blood. Specific sets of primers were used for amplification of gene fragments corresponding to the target SNPs. The genotyping was performed through a direct sequencing. Serum level of IL-2, IL-4 and TNF-α were measured using enzyme linked immune-sorbent assay (ELISA). AG genotype of TNF-α-308 G>A polymorphism showed higher frequency in BCa patients than controls (34.48% vs. 21.43%) with a significant difference (OR=2.6, 95%=1.13-5.9, p= 0.022).Allele analysis of IL-4-590 C>T polymorphism revealed a significantly higher frequency of the mutant allele (allele T) in patients than controls (37.07% vs. 21.43%) (OR= 2.16,95%CI=1.029-4.532, p= 0.039). Median serum level of TNF-α was significantly higher in patients (72.7 pg/mL) than controls (47.3 pg/mL). 12.9-417.4 These data strongly suggest that the significant role of TNF-α-308 G>A and IL-4-590 C>T polymorphisms as a risk factor for Bladder cancer in Iraqi patients. Different variants in this site may influence the expression of the corresponding cytokine. These polymorphisms increase susceptibility to Bladder cancer by altering the immune response of an individual.
144 MRI FINDINGS IN PATIENTS WITH HYPOGONADOTROPHIC HYPOGONADISM THAT ARE CHARACTERISTICS OF KALLMANN SYNDROME, Ali Hussein Albayati, Nihad Abdallah Selman, Zaid Hadi Khadum
One of the congenital disorders that is characterized by reduced or even absent sense of smell, hyposmia or anosmia, is Kallmann syndrome. Actually, the principal problem is hypogonadism since this congenital disorder is characterized in addition to smell abnormality by hypogonadotrophic hypogonadism. In addition to hormonal assays, imaging techniques and mainly MRI plays an important role in establishing the diagnosis. In this Iraqi study, 20 patients with hypogonadotrophic hypogonadism have been examined by MRI in order to identify features that are caharcetristic of Kallmann syndrome contrasted to idiopathic hypogonadotrophic hypogonadism. The current study was carried out in the radiology unit at AL-Hilla Teaching Hospital, Babylon province, Iraq. The study was carried out in cooperation with urology unit. The study included a series of 20 patients who were referred by urology department for the assessment of cause of hypogonadotrophic hypogonadism following initial suggestion based on hormonal levels, FSH, LH and testosterone. The age range of those patients was form 20 to 27 years. The MRI imaging included coronal, sagital and axial T1 and T2 weighted images. The thickness of images was 3 mm and the interslice gap was 0.3 mm. Performance of MRI imaging was carried out using 1.5 T GE system. According to MRI examination, there were 5 patients with absent olfactory bulb accounting for 25 % and 3 with absent olfactory sulcus accounting for 15 %, as shown in figure 1. Twelve patients had no detectable MRI abnormality and therefore were considered to have idiopathic hypogonadotrphic hypogonadism; where those 8 patients with abnormal MRI findings were considered to have Kallman syndrome. Absent olfactory bulb was significantly asscoaited with anosmia. The principal findings in MRI that differentiate Kallman syndrome from idiopathic hypogonadotrophic hypogonadism are olfactory bulb and sulcus abnormalities that are best visualized using coronal MRI scanning.
145 Effect of using regional anaesthesia (Tap) block as a method of analgesia in cesarean section in Iraqi female patients: A postoperative study, Amani Alaa Saeed
Background: Effective and good pain management is important part during perioperative period of any surgery. Pain control is important because it is associated with lower level of outcomes and recovery such as delayed functions, poor breast feeding, more risk for postpartum depression with persisting pain. The aim of current study was to use regional anaesthesia under ultrasound guide as a way to decrease the need for more analgesia in postoperative period. Methods: Seventy patients selected for elective Cesarean Section under general anaesthesia prepared for the study at the time before end of effect of anaesthetic drugs. The patients were divided into two groups; ultrasound-guided TAP block (group A) which was done with 20mL lidocaine 1% on each side and the other group (group B) which had been given paracetamol 15mg|kg and diclofenac 75mg intravenously. Numeric rating scale was used to assess pain. Paracetamol was used if the score more than 3 and diclofenac was added when the score more than 6. Patients were monitored for numeric rating scale and whole analgesia utilized for the early 24 hour post-operatively. Results: Regional block with lidocaine decreased pain scores at 0, 2, 4, 6,12 and 24 hours. Also there was a difference in the scores at 0, 2, 4, 6,12 and 24 hours between the two groups. The duration of analgesia in TAP block with lidocaine lasted to about 20-24h. The whole analgesics utilization was also reduced in group A than group B. No complication was seen to TAP block in both the groups. Conclusion: Regional anaesthesia is good to do for patients undergoing surgery to lessen analgesic requirements especially in the early period post-operative with prolonged pain-free time and no extra need for injectable analgesia. Also, it provides better pain scores with less complications following Cesarean Section surgery.
146 MANAGEMENT OF HYPONATRAEMIA IN POST-NEUROSURGICAL PATIENTS, Yasameen RiyadhKhaleel Al-Saffar, Ken Ho, Min Ling
Hyponatraemia (serum sodium
147 IL-8 is a valuable laboratory marker for detection of amoebiasis severity, Raed Fanoukh Aboqader, Mohammad H Al-Hasnawy
Background: Amoebiasis is caused by Entamoeba histolytica and is an important human parasitic disease. IL-8, also known as neutrophils chemotactic factor, induces chemotaxis in target cells. The aim of current study was to assess using IL-8 as a laboratory marker for amoebiasis severity. Methods: Sixty three cases with clinical diagnosis of amoebiasis were selected in addition to 26 apparently healthy controls with negative diagnosis of amoebiasis. For both cases and controls, general stool examination using wet mount and assessment of IL-8 were done using IL-8 ELISA kit (Biosource ) according to manufacture instructions. Student's t-test was used to compare the mean and standard deviation and P value 0.05) while in those more than 15 years old, incidence of amoebiasis was 82.75% in males and 17.24% in females with statistical significance at P value
148 Prevalence of Viral Hepatitis Infections in Babylon Province, Iraq, during The Interval from 2014 to 2018, Mohammed Malih Radhi, Niran Kadhim F. AL-Rubaey, Rusull Hamza Kh. AL-Jubori, Nada Khazal Kadhim Hindi
Background: Viral hepatitis disease is a major public health problem affecting hundreds to millions of individuals worldwide. The most widely recognized types of viral hepatitis are six distinct types of hepatitis virus, referred to as A, B, C, D, E, and G which may appear in an acute or chronic form. The aim of current study was to to estimate the prevalence of hepatitis A, B and C virus infections over the last five years ago from 2014 to 2018 and to determine the prevalence associated with age and gender of patients in Babylon province, Iraq, through analyzing data by descriptive statistics approaches. Methods: Current study involved 2110 positive cases who were diagnosed as having hepatitis A, Band C virus infections of age groups ranging from (< 1 to ≥ 20) years of both genders (1184 males and 926 females). Patients' information was gathered retrospectively from the medical records at The Central Public Health Laboratory of Babylon province for the period between 2014 and 2018. Results and Conclusion: The study results showed that 1868 cases (88.5%) out of 2110 cases had Hepatitis A Virus while 205 cases (9.7%) had Hepatitis B Virus and 37 cases (1.8%) had Hepatitis C Virus. Also, the highest number of cases (653) of viral hepatitis infections was recorded in 2015 and the lowest number (307) was recorded in 2014. On the other hand, it has been discovered that the highest total number of cases was for Hepatitis A Virus cases (576) cases, followed by Hepatitis B Virus (60) cases and then Hepatitis C Virus (17) cases for year 2015. The analysis of results depicted the relationship between types of viral hepatitis infections and years of prevalence and revealed a highly significant association at P-value
149 The role of BipA in the regulation of K1 capsular polysaccharide production of uropathogenic Escherichia coli, Hasan A Aal Owaif, Ahmed A Mhawesh, Sura A Abdulateef
Background: K1 capsular polysaccharide is usually expressed in uropathogenic E. coli.The aim of current study was to evaluate the effect of BipA in the regulation of K1 capsular polysaccharide expression in E. coli. Methods: In order to identify the role of BipA in theK1 capsule expression,bipA gene was deletedin the UPEC strain UTI89.Results: The flow cytometry analyses showed that the capsular polysaccharide expressedon the UTI89ΔbipA cells was less thanthe capsular polysaccharide present on the wild type UTI89 cells. Also, plaque assays using K1-specific bacteriophage showed that the plaque diameter produced on UTI89ΔbipA was about 1/2 the plaque diameter produced on wild typeUTI89indicated a reduction in the capsular polysaccharide expression from UTI89ΔbipA in comparison with UTI89.Conclusion: These results showed that the expression of K1 capsular polysaccharide is up-regulated by BipA.
150 Efficacy of transdermal testosterone in assisted reproduction outcome of poor responders, Milal Muhammad Al-Jeborry
Background: Testosterone had a synergistic action with follicular stimulating hormone to enhance early stages of requirement and growth of ovarian follicles to enhance outcome in assisted reproduction program cycles. The aim of current study was to assess whether transdermal testosterone treatment before assisted reproduction cycle is effective in women with poor response. Methods: A prospective randomized trial performed in 132 low prognosis women for two years and at two IVF centers in Iraq. Participants were divided randomly into two groups; Group 1 involved 71 poor responders received transdermal testosterone in previous cycle and Group 2 women did not receive testosterone transdermal. In both groups, high dose gonadotrophins imitated from second day of cycle following baseline vaginal ultrasound and baseline hormonal investigations after obtaining written consents from all participants and ethical approval from assisted reproduction committee in Baghdad. The treatment regime was flexible antagonist in dose of 0.25mg corticosteroid when transvaginal ultrasound revealed dominant follicle size 13-14mm given daily subcutaneously till day of HCG triggers. Number of retrieved and mature oocytes and pregnancy rate were primary outcomes while secondary outcomes were fertilization and implantation rates, and rate of cancellation of cycle of IVF in addition to required doses of administered gonadotrophins and duration of stimulation. Results: There was no significant variation regarding the demographic parameters and basal hormonal assessment (P-value >0.05%). The testosterone gel group required less days of stimulation and fewer ampoules of gonadotrophins than non-testosterone group (P-value < 0.05%). There were more collected oocytes, mature and fertilized oocytes with more transferred embryos with reduced cancellation of IVF cycle in testosterone gel group compared to non-testosterone gel group (P-value < 0.05%). The pregnancy rate was none statistically higher in testosterone gel-treated group compared to non-testosterone treated group (P-value > 0.05). Conclusions: In poor responders the transdermal testosterone treatment in the preceding cycle of IVF improves the clinical outcome with more collected and mature oocytes, higher pregnancy rate and less cancellation.
151 Review of Attacks of Wheezy Chest in Infants in Relation to their Socio-Demographic Characteristics, Sijal Fadhil Farhood Al-Joborae
Background: Bronchiolitis is the blockage of small airways of the lungs due to viral infection. It usually affects children younger than 2 years and may present as fever, cough, wheezing and breathing difficulty. It is usually caused by respiratory syncytial virus or human rhinovirus. The aim of current study was to evaluate the geo-demographic risk factors for bronchiolitis in infants in Al-Hillah city, and provide analysis of both infantile and maternal variables that contribute to recurrence of bronchiolitis. It also aimed to impact local health authorities in Babylon to advocate breastfeeding, and minimize problems related to prematurity all of which lessen the financial burden of bronchiolitis. Methods: The study was a cross-sectional study conducted in Al-Hilla city, Babylon, Iraq, from 1st, November 2017 until 15th, March 2018. The study included 720 infants (one year of age or younger) with recurrent bronchiolitis who were residents of Babylon province. Data were collected using special questionnaire that included various demographic and clinical variables. Results: Mean±SD age of infants was 5.85±3.24 months with mean birth weight of 5813.3±2290.9. Majority of them had bronchiolitis in winter. There was significant association between crowding index and both timing and number of attacks (P
152 EVALUATION OF LEFT VENTRICULAR FUNCTION USING GLOBAL LONGITUDINAL STRAIN IN PATIENTS WITH SUBCLINICAL HYPOTHYROIDISM, Ali Hussein albayati,Safaa Jawad kadhem, Oday Jasima Salihi
By utilizing 2D speckle tracking cardiac echo, the current study aim is to assess any changes on the structureor function of heart muscle in previously healthy subjects with newly diagnosed subclinical hypothyroidism. Patients & methods: Forty consecutive symptomless patients with recently diagnosed untreated subclinical hypothyroidism who were attending our personal clinics and 40 age & sex matched healthy volunteers were randomly chosen (control group) registered within the study. All the patients in the current study were examined by standard 2D cardiac echo and speckle tracking echocardiography. The LV GLS and circumferential strain shown to be considerably lower in the patient’s group in comparison with the healthy subjects. Left ventricular systolic function derived from Speckle tracking echocardiography was impaired in patients with subclinical hypothyroidism in comparison with healthy subjects.
153 SERUM CHEMERIN CONCENTRATION AND CORRELATED WITH INSULIN RESISTANCE AND VITAMIN D IN IRAQI PATIENTS WITH CARDIOVASCULAR DISEASE, Shaker Abdelredha Abbas, Mohammed Zeger, Fataneh Fatemi, Hanaa Addai Ali, Seyed Omid Ranaei Siadat, Muthana Saleh Mashkur, Ali Naji Alnajm, Ali Husein Almoswy
Cardiovascular disease (CVD) is the the leading cause of morbidity and mortality around the world. Thus, early prediction andpreventionof CVD patients are crucial.The main patho- physiologic mechanism underlying the high mortality of CKD includes premature athero- sclerosis and chronic inflammation. The aim isto detect the concentration of serum chemerin in subjects with cardiovascular disease and its relationship to biochemical parameters of glycemic indeces insulin resistanceand insulin sensitivity. Acase-control study for 90 eligible participants were included and were divided into three groups including ( Atherosclerosisgroup = 30 ),( Cardio group =30 ), ( healthy group = 30 ).Chemical and biochemical parameters were measured in all participants. Serum chemerin level were higher in Atherosclerosis (6.78 ±1.51 (ng/ml ), P< 0.001 ) and Cardio group (4.00±1.10, P< 0.001 ) compared with those as a control group ( 2.37±0.84, P< 0.001 ), correlation showed that serum levels of chemerinanalysisin patiant with Atherosclerosis werea significantpositive correlation with BMI,FBG, insulin,Vit D,HOMA IR, TC,VLDL-c, TG, LDL-cand a negativesignificantof correlation with Age,QUICKIandHDL-c. While the results of the univariatanalysisfor Cardio group that serum chemerinconcentration were a significant positive correlated with Age, BMI,FBG,insulin,HOMA IR, TC, TG, LDL-c and VLDL-c andasignificantnegativecorrelation with QUICKI, HDL-c andVit D. The study concluded that serumChemerinlevels in patients of Iraqi compared with Cardiodisease were significantly increasedthan those in the healthy group, while serumChemerin levels wereelevatedin the Atherosclerosis group than in the Cardio disease group and levels of Chemerinwere positively correlated with obesity and insulin resistance indicatorsand negatively with QUICKI.
154 Prediction of fetal sex by early pregnancy ultrasound localizing the implantation site lateralization, Milal M Al-Jeborry, Suhaila F Al-Shaikh
The Prediction of fetal sex by early pregnancy ultrasound localizing the implantation site lateralization consider a very important step in investigation, in this work will be questionable results, the genetic factor, fetal sex determination might be influenced by other factors. Why male sex has a predilection for right-sided implantation while female sex implanted on the left side of the uterus so this study aimed to assess the accuracy of fetal sex prediction by localization of the implantation site at 5-7weeks of gestation using the two dimensional trans-abdominal U/S.
155 Determining the effect of antigens prepared from kiwi fruit on allergic patients in Basra province, Iraq, Shayma'a Jabbar Raisan, Huda Kadhim Kareem, Saad S Mahdi Al-Amara
This study aimed to determine total IgE in subjects under study and controls an estimation of the specific IgE antibody response against kiwifruit antigen by ELISA assay. There are many studies on against kiwifruit in other countries of the world, but this study differs in sample type and Geographical location. A total of one hundred twenty blood samples from allergic patients (41 Males and 55 female) with age group (15-69) years were tested by direct and indirect Enzyme-linked immunosorbent assay for total and specific IgE antibodies against kiwifruit antigens. In our result, we show that the total IgE 100>IU/ml for patients had a higher rate of (72.9%) with significant difference (p
156 Bone morphogenetic proteins (BMP-2, BMP-7, and BMP-12) and chitotriosidase as novel markers in detection and staging of breast cancer in Iraqi women, Zahraa M Ali, Shatha H Ali, Furat Y Mohsen
Breast cancer (BC) is the most commonly diagnosed cancer in women, bone morphogenetic protein (BMPs) are highly correlated to various aspects of carcinogenesis, whereas chitotriosidase is chitinase that mediates several processes and synergistic effects with proteases and other enzymes to kill different types of pathogens and cancer cells. The present study was aimed to assess the validity of the measurement of several BMP as tumor markers aid in detection or staging of breast cancer. These include three types of growth factors: (BMP-2, BMP-7 &BMP-12) besides chitotriosidase enzyme levels in Serum. This study included 66 women with breast cancer. Those patients were categorized into three groups (22 in each group) according to disease stage, in addition to group 4 and chitotriosidase were estimated quantitatively using ELISA kits. Data analysis revealed that although serum levels of studied BMPs were not significantly different among studied groups, serum BMP-7 of patients at stage III presented with significantly higher levels than the controls, while serum BMP-12 levels were lowered significantly at stages I &II breast cancer patients as compared to the controls. Furthermore, elevated serum chitotriosidase levels were only detected at stage III levels only as compared to the controls. Thus both serum BMP-7 and chitotriosidase can be recommended as markers for prognosis of BC to advanced stages (stage III), while serum BMP-12 is recognized as a marker to identify patients with breast cancer at early stages (I &II).
157 Molecular detection of Salmonella typhi isolated from diarrheal patients in Al- Najaf governorate, Ahmed Al Obaidi, Najat Mohammed Flyyih , Mohammed Abdulrazzaq Assi, Basima Basim
All of (80) stool samples isolated from affected persons with diarrheal problems from) 11/ 2018 to 12/ 2018). All samples tokened from patients admitted to AL-Sadder Medical hospital and AL-Hakem General Hospital through the studying time. The samples were grown on the S-S agar media and incubated for 18 - 24 hr.at 37°C with the aerobic condition. About 80 patient samples, results include 38 (47.5%) were males and 42 (52.5%) were females. Many cultural, morphological, and biochemical tests were made in order to recognize bacterial isolates. Results revealed that Salmonella typhi constitute twelve (12) isolates from all specimens. A collection of 12 (15%) Salmonella typhi isolates demonstrated by the cultural, biochemical and morphological features, the investigation was specified by molecular techniques to investigate the fliC gene presence. The results revealed that only 6 (50%) samples were carrying the fliC gene which is considered as Salmonella typhi. The assays of antibiotic susceptibility test showed that samples of Salmonella typhi exhibited complete sensitivity to Gentamicin and Ciprofloxacin, moderate sensitivity against Trimethoprim, whereas the findings showed complete resistant to amoxicillin and piperacillin. According to these results it can have considered that Gentamicin and Ciprofloxacin as better choice antibiotics for treatment against Salmonella typhi.
158 Designing of molecular tool for the detection of helicobacter pylori in Iraqi patients using multiplex PCR technique, Karam D Salman, Amina N Al-Thwaini,, Ilham A Khalaf, Bassim A Askar
Helicobacter pylori colonize the gastric mucosa of more than 60% of the world’s human population. This bacterium plays a significant role in the pathogenesis of different diseases of the digestive system, such as chronic gastritis, peptic ulcer, and gastric adenocarcinoma. Accurate diagnosis of H. pylori infection is very important in the effective management of many gastroduodenal diseases. There is no gold typical technique that is been well-known for the detection of H. pylori infection. A multiplex polymerase chain reaction (mPCR) for glmM and 16S rRNA genes was established in our study for sensitive detection of H. pylori from gastric biopsies. Different classical detection techniques have been used lately with mPCR like Rapid Urease Test (RUT), histology and antibody (Serology) test. Detection of housekeeping (HK) genes by monoplex and multiplex PCR with different sets of primers for 16S rRNA due to heterogenicity and high variability in this gene. Our results show that a total of 123 (58.5 %) from 210 patients were positive for H. pylori infection. H. pylori were detected in 46.6% (98/210) by RUT, 54.7% (115/210) by histology, 85.7% (180/210 false-positive results were included) by H. pylori IgG, and 57.1% (120/210) through mPCR. By this molecular technique, H. pylori were detected in 100% of biopsies with positive histology and RUT. Our Conclusions prove that the mPCR was able to detect the highest numbers of positive cases although the lowest average scores for inflammation and activity.
159 Impact of Extracorporeal Shock Wave Therapy on Spastic HandFunction with Assistive Devices in Children with Cerebral Palsy, Sadiq Nassir Farhan, Siham Sabah Abdullah, Firas Mohamed Abdulgani
Background: The spastic cerebral palsy isconsidered the most common of cerebral palsy types. Spasticity of upper limbs is major problem in children that affected on hand function during grasping and through using assistive devices as walker and canes. Shock wave treatment on spastic muscles of upper limbs in stroke patients performed a significant decrease in muscle tone.The aim of current study was to determine the impact of extracorporeal shock wave therapy (ESWT) on spastic muscle of hand in children with spastic cerebral palsy.Methods:Thirty twohemiplegic spastic cerebral palsy patients from both sexes were recruited in this study.The children were divided randomly into two groups equal in number; GroupӀ(control)consisted of 16 children(7 boys and 9 girls) with mean±standard deviation age of 6.68±2.63years received exercises program only (3sessions/week for two months, GroupӀӀ(experimental)included (10 boys and 6 girls)with mean±standard deviation age of 6.37±1.44 years received the same exercises program in addition to sessions by extracorporeal shock wave therapy(BTL-6000WT) with energy flux(0.003mJ/mm²),frequency (10Hz)and (one session/week) for 8 weeks .The two groups were evaluated before and after therapy by Modified Ashworth Scale (MAS) and Modified House function Classification (MHC).Results:The results showed a statistically significant improvement in spasticity and hand function post treatment.Conclusion:We concluded that the ESWT is effective in decreasingspasticity(muscle tone) of upper extremities and enhancinghand function for patients with spastic cerebral palsyto help those children become independent and participate in different daily activities as grasping of walker or canes.
160 Investigation of Chitosan/PEO Reinforced with AgNPs for Antibacterial Activity Prepared by Solution Casting Method, Hanaa Shuker Mahmood, Mohammed Kadhim Jawad
Background: Chitosan (CS) is a natural environmentally friendly polymer. It is a linear polysaccharide of random distribution. The aim of current study was to study the effect of weight ratioon structural properties of the chitosan/PEO polymer, using XRD and FTIR measurements,and to find the optimum ratio with the highest mechanical and antibacterial properties of chitosan:PEO blend, which was prepared by casting method. Methods: In this work, chitosan and chitosan/PEO blend with different PEO ratios (5, 10, 15, 20 and 25wt%) were prepared by solution casting method. The prepared samples were examined by x-ray diffraction (XRD), Fourier-transformed Infra-red spectrometer (FTIR) and tensile tester. Also,the antibacterial activitiesof prepared blends at different ratios were evaluated using half maximal inhibitory concentration technique against Escherichia coli and Staphylococcusaureus microorganisms. Results: The XRD and FTIR measurements indicated the formation of the blend by the obvious change in their patterns. The best mechanical properties appeared at 15% PEO ratio. It was found that the highest inhibition of bacteria for Chitosan/PEO blend was at 90:10 blend ratios. The blend has less effectiveness against Gram-positive (Staphylococcus aureus). The optimum blend sample, in terms of mechanical properties, was reinforced with different silver nanoparticles (AgNPs)(1, 2, 3, 4 and 5wt %) to find its effect on the structural and antibacterial activity.Conclusion: It was found that blend reinforced with AgNPs had clearly improved in its effectiveness as an antibacterial substance, especially against Gram-positive bacteria.
161 The roles of Human Cytomegalovirus and Epstein-Barr virus in Type 1 Diabetes Mellitus, Al Zahraa Al Batool Ibrahim Saber, Ahmed Hasan Mohammed
Background: Type-1-diabetes, also known as insulin-dependent diabetes mellitus, results from the progressive destruction of pancreatic beta cells resulting in insulin deficiency.Studies on the risk of developing Type-1-diabetes suggested that environmental factors, particularly viruses, may be involved in initiating the destruction of beta cells leading to Type-1-diabetes. The aim of current study was to investigate the possible correlation between human cytomegalovirusand Epstein Barr virus with type 1 diabetes mellitus.Methods: A total of 56 patients diagnosed with Type-1-diabetes who attended the Special Center for Endocrine Glands and Diabetes in Al-Nassyrieh city, and another control group of 30 non-diabetic healthy people were selected. These two groups were of age range 3-22 years old and from both sexes. The sera from both groups were collected and divided into two parts. One part for the serological detection of IgM and IgG antibodies against human cytomegalovirusand Epstein Barr virus by the enzyme-linked immune sorbent assay (ELISA) and another part to detect the viral target genes of human cytomegalovirusand Epstein Barr virus genome by the conventional Polymerase Chain Reaction(PCR) technique.Results: The results revealed that only 2(3.60%) of Type-1-diabetes patients were positive for anti-human cytomegalovirus IgM antibodies compared with a negative result of the control group and 53(94.60%) of Type-1-diabetes patients were positive for anti-human cytomegalovirus IgG antibodies compared with a full percentage in the control group (100.00%). Also, ELISA results indicated the presence of anti-Epstein Barr virus IgM antibodies and anti- Epstein Barr virus IgG antibodies in 7(12.50%) and 24(42.90%) of Type-1-diabetes patients, respectively, compared with negative results for both anti- E[stein Barr virus IgM and IgG antibodies of the control group. Regarding PCR technique, the results revealed that (33.93%) of Type-1-diabetes patients had Human Cytomegalovirus DNA and (26.79%) of Type-1-diabetes patients hadEpstein Barr virus DNA compared with none of the controls had Human Cytomegalovirus DNA or EpsteinBarr virus DNA (P
162 School Achievement of Primary School Children with Type 1 Diabetes Mellitus in Baghdad, Sarah Hayder, Farsi Al Lami, Khalid Saeed
Background: Children spend many hours a day at school and the overall goals for children in these settings are to maintain excellent glycemic control and minimize interruptions of their daily learning. The aim of current study was to assess the school achievement of primary schoolchildren with Type 1Diabetes Mellitus in Baghdad and identify the main factors affecting this achievement.Methods: This comparative cross-sectional study was conducted in a sample of primary schools in Baghdad City selected by multistage cluster sampling. All primary school students in the selected schools were included. For every diabetic child, we selected a child from the same class who is free from diabetes. Information on school achievement, sociodemographic variables and disease history were obtained through interviewing the children and their parents. Poor performance was considered if the student had grade point average 10 days or had a school year repeated.Results: Diabetic group had significantly higher number of absenteeism days than non-diabetic group (P
163 Distribution of quorum sensing genes and typing of clinical Acinetobacter baumannii isolates by ERIC-PCR, Anfal Mohammed Khudhair, Ali H. Alsakini
Background: Acinetobacter baumannii has arisen as disturbing nosocomial pathogens between Iraqi hospitals inpatients.The aim of current study was to determine if the increase of A. baumannii incidence in patients on blaOXA-51 gene in A. baumannii that carry QS gene showed pathogenicity of clinical isolates and to determine the efficiency of ERIC-PCR fingerprinting method for genotyping of A. baumannii. Methods: Sixteen isolates diagnosed as A. baumannii and genetically confirmed by blaOXA-51 as a marker gene from different clinical sources in Baghdad hospitals.The virulence of the A. baumannii does not require, carrying full set of QS ( LasRand RhlI, LasI, RhlR) genes.Results: The positive QS genes results were distributed from high to low expression, lasI 75%(45/60),70%(42/60)for RhIR,50%(30/60) for rhII, and 13.3%(8/60)for LasR. Using fingerprinting ERIC-PCR analysis, 57isolates of A. baumannii were clustered into 2 groups while the remaining 3 were single isolates. The genetic linking of A. baumannii isolated from different hospitals inpatients was high, indicating horizontal gene transfers within hospitalized patients. Conclusion: Our findings indicated accurate and fast diagnosis method to detect virulent A. baumannii isolates harboring differing sets of QS by using blaOXA-51 gene and ERIC-PCR for genetic variations, respectively, possible to be helpful with epidemic infections.2
164 ASSOCIATION BETWEEN AUTOPHAGIC FLUX AND INFLAMMATION MARKERS IN RHEUMATOID ARTHRITIS PATIENTS RECEIVING METHOTREXATE AND INFLIXIMAB, Saja Zohaa Flyeh, Ghassaq Tariq Alubaidi, Mohammed Hadi Alosami, Hiader Faisal Ghazi, Ilham Ahmed Jasim, Israa Wajeh Ahmed
Autophagy is a primordial form of eukaryotic innate immunity which arises as a cellular response to stress conditions such as growth factors withdrawal, mitochondrial and other organelles damage, nutrients deficiency and inflammation and assists in removal of damaged organelles. This study aims to investigate the role of autophagic flux (LC3ІІ/І) in rheumatoid arthritis in terms of disease severity represented by DAS-28 and immunological markers represented by TNF-ɑ and NLRP-3 also to study the possible effect of certain rheumatoid arithritis drugs (MTX and infliximab) on autophagic flux. The present study involves sixty rheumatoid arthritis patients, thirty of them receiving chemical drug (MTX) and other thirty receiving biological treatment (Infliximab), comparing results to healthy control group consist of thirty people. These two groups of rheumatoid arthritis patient were subjected to the molecular measurement of the autophagic flux level presented by LC3II/I ratio and NLRP3 level by (rtPCR) and serum TNF-α level by indirect ELISA. Autophagic flux presented by LC3ІІ /LC3І ratio, showed a highly significant correlation with the DAS-28, NLRP-3 and TNF-ɑ level,0.577 and 0.855, 0.640 respectively, P value ˂ 0.001.The autophagic flux in the MTX patients was 2.81 comparing to 1.91 in the Infliximab group,P value is ˃ 0.001 pointing to the absence of any significant difference between them. This study referred to the presence of a detectable correlation between autophagy and rheumatoid arthritis severity presented by both clinical and immunological aspects, on the other hand both of MTX and Infliximab seem to affect autophagy in a similar strength
165 INCIDENCE AND CORRELATION OF INTERFERON-GAMMA ASSAY AND ANTI-RUBELLA ANTIBODIES TO DETECTION RECURRENT MISCARRIAGES PREGNANT WOMEN WITH LATENT TUBERCULOSIS INFECTIONS IN AL- DEWANIYAH GOVERNORATE, IRAQ, Haider Abed Ali Alshawi, Alkarrar Kais abdul Jaleel Duaibel, Muneer A. Jawad Al-Tumma
Tuberculosis (TB) disease and Rubella virus adversely affect Latent Tuberculosis Infection (LTBI) in pregnancy and major cause of maternal-child mortality. The aim of this study was undertaken to perform cross-sectional research to evaluation agreement and a correlation between an IFN- γ releasing assay for detected latent Tb infection and anti-rubella antibodies tests in pregnant women with recurrent miscarriages. Method: the study was carried out between 1 March 2018 Until 1 July 2019.Study groups: A total of 75 pregnant women living in TB-affected areas were in direct or indirect contact with an infected person from their families or relatives and had a history of recurrent miscarriage in the first months of pregnancy, their ages ranged from (18-41) years. There screened for Rubella IgG and IgM and IFN- γ release assays (IFN- γ RAs). The research showed a following results, Anti-Rubella IgG, IgM (positive) according to recurrent miscarriage, number and percentage of Rubella IgG, IgM positive was (36)(48%);(22)(73.3%), (4)(5.3%); (0)(0.0)in both groups(study and control) respectively, and significant P value> 0.05. The Quantiferon test in different study group and control group according to age groups showed that out of recurrent miscarriage pregnant women have positive result 4 (5.33). Comparison the results of two tests of Quantiferon release IFN-γ assay and Anti-rubella virus antibody (IgG, IgM) that had significant relationship P= 0.0443. Conclusion of our findings indicates whether any of these tests should be done and how sensitive they are a great difference in test results when used between recurrent miscarriages pregnancy.
166 DETERMINATION OF NUMERICAL INDICATORS TO ESTIMATE THE COMPONENT OF THE THINNEST BODY AND ITS LINEAR RELATIONSHIP TO LACTIC ACID ACCUMULATION AND THE ACCURACY OF THE PERFORMANCE OF SOME BASIC SKILLS IN TENNIS, Makki Jabbar Oudah Al. Majidi, Hutham Abdulameer Ameen
Each sports activity has special physical requirements that need to be met. This is part of the great interest in sports and what constitutes tennis sport of great interest in the world of sports and through observation and awareness of the researchers, including March the game of tennis ground and taught and taught in practical lectures These topics, especially with respect to the methods and measurement of anthropomorphic body style, are of great interest to many foreign scientists who designed special equations and tables that help the reader to know the pattern of his body and went to the study of the human body in terms of its shape and size, The researchers studied the components of the athlete's body style for their relationship with many kinetic abilities and indicators of superiority, which can be used to select the players and players according to the type of sport activity as well as the knowledge of its linear relationship with accumulation Lactic acid and the accuracy of the performance of some basic skills in ground
167 GENETIC ROLE OF HLA-G14 BASE PAIR INDEL POLYMORPHIC GENE WITH TYPE 1 DIABETIC PATIENTS, Hasanain Khaleel Shareef, Ahmed Adil Ali, Rafah F. Al-Jebori
The prevalence of Diabetic mellitus in Iraq is high for both males and females, according to the World Health Organization (WHO). The major histocompatibility complex (MHC)/HLA region on chromosome 6p21 has been shown to contain the major genetic component of Type1Diabetic mellitus. A 14-base pair polymorphism inserts and/or removal in exon-8 has a potential role in HLA. This research explores the role of 14-bp HLA-G insertion / deletion polymorphism in Type 1 Diabetes mellitus (T1DM) patients. The polymorphism allele frequency was calculated in patients with T1DM and control. Insertion allele (70.8%) and homozygous deletion genotype are associated with T1D susceptibility (51.6%), while control group (38.3%) and heterozygous genotype of the 14-bp indel are correlated with T1D defense (38.3%) and control group (50%). also a significant differences in the allele frequencies of the HLA-G 14-bp polymorphism were observed. This research shows a sturdy relation among polymorphism HLA-G 14-bp and type1D.M. (P = 0.009). Our findings describe the combination of the 14-base pair insertion allele and the homozygous genotype deletion to the progress of T1D.
168 GENETIC ROLE OF HLA-G14 BASE PAIR INDEL POLYMORPHIC GENE WITH TYPE 1 DIABETIC PATIENTS, Hasanain Khaleel Shareef, Ahmed Adil Ali, Rafah F. Al-Jebori
The prevalence of Diabetic mellitus in Iraq is high for both males and females, according to the World Health Organization (WHO). The major histocompatibility complex (MHC)/HLA region on chromosome 6p21 has been shown to contain the major genetic component of Type1Diabetic mellitus. A 14-base pair polymorphism inserts and/or removal in exon-8 has a potential role in HLA. This research explores the role of 14-bp HLA-G insertion / deletion polymorphism in Type 1 Diabetes mellitus (T1DM) patients. The polymorphism allele frequency was calculated in patients with T1DM and control. Insertion allele (70.8%) and homozygous deletion genotype are associated with T1D susceptibility (51.6%), while control group (38.3%) and heterozygous genotype of the 14-bp indel are correlated with T1D defense (38.3%) and control group (50%). also a significant differences in the allele frequencies of the HLA-G 14-bp polymorphism were observed. This research shows a sturdy relation among polymorphism HLA-G 14-bp and type1D.M. (P = 0.009). Our findings describe the combination of the 14-base pair insertion allele and the homozygous genotype deletion to the progress of T1D.
169 The effect of Ramadan fasting on body weight, fasting blood sugar and lipid profile of normal healthy and non obese male medical college students in Tikrit city., Abdulrahman Jihad Mansoor
Background: Fasting during the month of Ramadan is one of the five fundamental pillars of Islamic practices, mandatory for all healthy adult Muslims. In Ramadan, Muslim adults fastfrom sunrise to sunset and are required to refrain from oral intake of food, water, beverages, smoking and sexual intercourse. This type of fasting is defined as periodic food and water deprivation during day light hours with free access during the night for the duration of one month.The aim of the study is to The effect of Ramadan on body weight, fasting blood sugar and lipid profile of normal healthy none obese male medical college students in Tikrit city. Subjects and methods: A prospective study was carried out on thirty normal healthy male students aging between 20 to 22 years, in college of medicine-Tikrit university were participate in the present study. The volunteers were allowed to consume whatever they wanted & decrease fat intake in diet.The study was conducted in the month of Ramadan from beginning of May to 5th of June /2019. Average duration of the fast was about 13.5 hours & maximum temperature ranged from 25 to 35 C.Body weight was measured to the nearest 100gm. Fasting blood sugar and lipid profile were measured at beginning and the end of Ramadan and one hour before break fasting (one hour before sunset). Results: There were slight decrease in body weight and body mass index (BMI) at the end of Ramadan fasting as compare with beginning of Ramadan fasting. Also, in regard to blood parameters, there is no significant difference regarding blood glucose from at end of Ramadan as compare with the beginning of fasting. Also, there were significant reduction in serum TG, total cholesterol, LDL and VLDL at the end of Ramadan fasting as compare with beginning of Ramadan fasting. However, There was significant elevation in serm HDL at the end of Ramadan fasting as compare with beginning of Ramadan fasting
170 A comparison of Motor Intelligence and Some Basic Movements According to the Time of Daily Practice of Electronic Games and Watching Television for Children (4-6) Years, Abdelrahman Ibrahim , Omar Fadhil Yahya AL-Badri, Mohammed Saleh Khaleel Alsamarae
The study aimed to identify the time of daily practice of electronic games and watch television for children aged (4-6) years, and to identify (motor intelligence) for the child and some basic movements represented by (running, jumping, throwing) according to the variation of the times of daily practice of these electronic games and watching Television The sample consisted of (396) children enrolled in kindergarten in the city of Samarra in Salah al-Din Governorate in Iraq. The researchers concluded that children practicing these electronic games and watching TV with a time (less than two hours a day) showed superiority over their peers Marcin those games and TV viewers for more than (two hours) per day in all kinetic intelligence tests as well as in the evaluation of the results of the virtual form of basic movements of the children of both (running, jump, throw).
171 Examination of Immuno-pathological changes in Diabetic Mice , Sabrin Ibraheem Mohsen, Taghreed Jabbar Humadi, Muna Sachit Hashim
Background: Diabetes mellitus is a metabolic disorder a major health concern today whose prevalence has continuously increased of the global population. Alloxan-induced diabetes is used model to induce Type I diabetes mellitus in the experimental animals are one of the best strategies for the relative with immune responses of diabetic mice, It has been found toxic to pancreatic beta cells due to structural similarity to glucose analogues that is capable of inducing type I diabetes mellitus in experimental animals. Objectives: Examination immune-pathological changes in different organs after inducer diabetic in mice and determination of cellular immunity through 2 tissue markers (TNF, Glucagon). Methods: study design was animals randomized, in order to study the patho-immunological effects of induction of diabetes in mice (tissue damage biomarkers). Twenty one mice(100-120 g) were used in this experiment, those subdivided into three groups, first group (diabetic group)contained seven mice were injected intraperitonial at daily does for 6 weeks of alloxan (Alloxan monohydrate 150mg/Kg at single dose of 0.1ml/10mg B.W.),second group (immunized group) contained seven mice were stimulated immune response by vaccine of Brucella melitens is Rev.1 at dose 0.2ml/mouse subcutaneously at 2 doses between 2 weeks interval, third group (control group) contained seven mice were injected at daily dose of physiological saline solution. Results: Immunological analysis were done by use two cellular marker (TNFα alpha and Glucagon).Diabetic mice appear grossly very sick after Alloxan's injection and microscopically necrotic tissues with vacuoles within cytoplasm due to degeneration, mainly in liver; kidney as well as spleen showed lymphoid atrophy and skin showed loss of hair follicles with thickening epidermis and dermis layers. In immunized group mice appear similar to control group in activity grossly, and microscopically low level of pathological changes were appeared. Conclusion: Microscopic immune-pathological investigation of liver; kidney as well as spleen tissues showed marked significant decrease appear degenerated with a significant increase level of cytokine (TNF-α) and decrease of glucagon which could have the ability to regenerate tissue, cytokine repairing of necrotic tissue in early duration of diabetes metabolic. Pathological and immunological methods of inducing type II diabetes mellitus in experimental animals by drug-induced diabetic (alloxan) in skin, spleen, kidney and liver, that alloxan caused of immunopathological changes from skin, spleen, kidney and liver that lead to associated of someone of cytokines activation in the body with immunization.
172 The Difference In Sensory Latency Of Median-Radial Nerves In The Diagnosis Of Carpal Tunnel Syndrome, Zahid M Kadhim, Basim A Abd
Background: Electro-diagnosis plays an important role in the diagnosis of carpal tunnel syndrome (CTS);there are multiple tests available each with different sensitivity and specificity. Objective: This study aims to test the sensitivity and specificity of median-radial sensory latency comparison for diagnosing patients with early CTS .Methods: Hundred patients diagnosed clinically as having CTS with negative ordinary nerve conduction study were included in the study and tested for sensory nerve responses of median and radial nerves recorded from thumb and the difference between measured latency was measured. These patients are matched to 100 normal healthy volunteers. Results: The study found that the values of median sensory and motor latencies show statistically significant difference between patients and control. Also median sensory latency recorded from thumb finger was prolonged in comparison to that of radial nerve recorded from the same finger. Median-radial sensory latency comparison reveal high sensitivity (85%) and specificity (92%).Conclusion: Median-radial sensory latency comparison is helpful in the early diagnosis of CTS and is considered as complimentary part in the electro-diagnosis of CTS.
173 Trace Elements in Pregnant Women from Babil Province, Iraq , Alaa Sadiamza Al-Dujaily, Najlaa Badr Alawadi
Trace elements are essential for the proper growth, development and physiology of the human body. Pregnant women and fetus are at risk for trace elements deficiency, which is known to have negative effects on vital metabolic, immunological and physiological activities. This is a case-control study that investigated number of trace elements in pregnant women from Babil province. These women were divided into 3 groups, each one consisting of 100 women. Women in 1st, 2nd, and 3rd groups were in 1st, 2nd, and 3rd trimester of pregnancy respectively, a 4th control group were taken which consisted of 100 apparently healthy age-matched and non-pregnant women. This study found that iron deficiency is evident in both pregnant (43.3%) and non-pregnant (24%) women but become more prevalent and severe as pregnancy progress (33%, 41% and 56% in 1st, 2nd and 3rd trimesters respectively). Selenium was deficient in only 2% of control group but became deficient in 19% of 3rd trimester (P=0.0001). Serum calcium was deficient in 12% of 3rd trimester group versus 5% in control (P=0.001). No significant deficiencies were found in serum levels of iodine, zinc and copper. We conclude that iron and calcium deficiency are prevalent in pregnancy and both elements need to be replenished, and on the other side, selenium is also deficient and it's replacement need to be assessed in future studies.
174 Antimicrobial activity of silver nanoparticles biosynthesized by Streptomyces spp., Donaa Hamza Khair-Allah, AlAl-Charrakh,, Nawfal H Al-Dujaili
Nanobiotechnology is a new emerging discipline of nanoscience created by pairing of biotechnology, and nanotechnology. Silver nitrate (AgNO3) was used as precursor for biosynthesis of silver nanoparticles by Streptomyces spp.Silver nitrate was added with concentration 5 mMto biomass of Streptomyces spp. (MU-43 &SA-65)which distributed in sterilized flasks containingISP4 broth medium.This step was carried out under dark condition to avoid oxidation of AgNO3.Antibacterial activity of AgNPs were biosynthesized by each of the two isolates of Streptomycesspp(MU-43and SA-65) were examined for their antimicrobial activity against different types of pathogenic bacteria isolated,including both Gram positive and Gram negative bacteria were determined by disc diffusion method Antibacterial activity against tested bacteria was observed to be different regarding the source of biogenic AgNPs applied. The results showed that gram negative bacteria were higher sensitive than gram positive bacteria to AgNPs. Furthermore AgNPs fabricated by Streptomyces MU-43 isolate had higher activity than AgNPs fabricated by Streptomyces SA-65 isolate. Synergism among antibiotics-silver nanoparticles biosynthesized by both Streptomyces strains (MU-43 & SA-65) showed that this synergism was efficient to inhibit the tested bacteria.
175 Antibacterial and Cytotoxic Effects of Silver Nanoparticles on Staphylococcus aureus and Normal Vero Cells, Rasha Hadi Saleh, Entisar J. Al-Mukhtar, Zaytoon A. Al-Khafaji, Mohammed H. Al Hasnawy , Huda H. Al-Hasnawy
Silver(nanoparticles)(AgNPs) are of special concern as a result of their unique chemical, physical and biological characteristics. It has become an attractive alternative to antibiotics due to their broad-spectrum antimicrobial activity. The study aimed to determine the antibacterial activity of AgNPs against S. aureus bacteria and the effect of AgNPs on the-viability of normal cell line (vero cell). A1total of 70 clinical 1samples (wound and vagina swab, stool and urine)1were used in this study. Bacterial isolates were subjected to the microscopical, cultural and biochemical evaluation. AgNPs were-prepared and checked for their antimicrobial activity by the use of 1various concentrations employing agar dilution method. In addition, the effect of different concentrations of AgNps on a viability of vero cells was examined. The results showed that out of 70 clinical samples, 11 (15.7%) isolates were Staphylococcus aureus. AgNps showed high activity against S. aureus at concentrations (100 μg/ml and 200μg/ml). It was found that there was no effect of AgNPs on the viability of the normal vero cells at (≤ 250 μg/ml) concentration, but they have cytotoxic effect on the viability of the these cells at high concentrations. This study concluded that AgNPs possess good antimicrobial-activity and the concentrations that maintain the cell viability could be used as an alternative therapy to treat S. aureus infections.
176 Periconceptual prednisolone therapy for the management of recurrent pregnancy loss, Nadia Mudher S Al-Hilli
Background: Recurrent miscarriage has been investigated for a long time with different types of therapeutic trials for those with unknown cause. Prednisolone as an immune modulator agent can have beneficial effect in improving pregnancy outcome in those patients. Objective: The aim of this study was to investigate the role prednisolone in preventing miscarriage in patients with recurrent pregnancy loss. Methods: Sixty two patients enrolled in this study, all of them have recurrent miscarriage, after investigations and exclusion of those with antiphspholipid syndrome, medical disease, endocrine disorder and uterine pathology. Patients divided randomly and equally into two groups. Study group give prednisolone therapy 5 mg orally for two months prior to conception & continue during pregnancy till 13 weeks of gestation, while the second group received folic acid 5mg orally before conception and continue till the end of first trimester. Patients were followed up throughout pregnancy till delivery.Results: There was significant association between pregnancy outcome and type of treatment majority (90.3%, n=28) of patients using prednisolone continue treatment, while only (38.7%, n=12) of control group continue their pregnancy. (X2=18.03, P=
177 Determination of K, Na and Ca with flame atomic emission spectroscopy and screening of active compounds of Withania someniferum roots from Iraq, Nehad Kanaan Abed , Ali Rasool Mahmood Al-Bakaa , Mohammed Abdullah Ahmed, Zainab Abbas Jabbar
A soxhlet Carbon tetrachloride, Chloroform, Ethanol and Aqueous extracts from the W.S. roots (In Iraq as knowing semalferahk), which traditionally used for treatment of infertility; Alkaline elements (K) potassium, (Na) sodium, and (Ca) calcium were calibrated by F.A.E.S., and screening of active compounds of W.S. roots, to analyze Q.P. of various Carbon tetrachloride, Chloroform, Ethanol and Aqueous extracts of root parts of W.S. The present study investigates the qualitative analysis of the major bioactive constituents of root parts of W.S. in some solvents (Carbon tetrachloride, Chloroform, Ethanol and Aqueous water); Phytochemical screening method was used to identify qualitative analysis of bioactive component. While the quantitative analysis of (K, Na, and Ca) was analyzed by F.A.E.S.; The extraction results show higher concentration of potassium then sodium; While calcium doesn’t exist. The bioactive component flavonoids were found in all those extracts; but only CCl4 and CHCl3 extract contents saponin, while tannin and unthioqunone doesn’t exist in four extracts.
178 The satisfaction of patient with removable partial denture therapy from point of the comfort and chewing efficiency, Zainab Mahmood Al-Jammali, Azad Al-Muthaffer, Anas Al-Yasiry, Zahra Saad
The aim of this study was to assess relation of satisfaction level of RPDs related to comfort and chewing efficiency of patients who seeking treatment of removable partial denture. Thirty two patients with a removable partial dentures, were examine, age range (30-60) years. After construction of the prosthesis, we ask the patients questions about comfort and chewing efficiency and the satisfaction of patient was evaluated by using scale range from 1 to 5.Results found that the largest percentage of the female were satisfy (55%) with the comfort of her denture compared with the male patients and the differences significant. The largest percentage recorded in the employed patient (52.6%) were satisfied compared with the non-employed patient who have (46.2%) were satisfied. About chewing efficiency, the largest percentage recorded for the male patient (50%) was satisfied compared with the female patients. Among age groups, the largest percentage was for the G.I satisfy compared with the G.II. The largest percentage recorded in employed patients compared with the non-employed patient.About comfort, the largest percentage of the female were satisfy of her denture compared with the male patients, the largest percentage for G.II was satisfy compared with the G.I, and the largest percentage recorded in the employed patient were satisfied compared with the non-employed patient. About chewing efficiency, the male patients were more satisfied than female patients, in relation with age, the largest percentage was for the G.I satisfy compared with the G.II, and the largest percentage recorded in employed patients were satisfied compared with the non-employed patients.
179 Combination Therapy with Rituximab and Methotrexate in the Management of Rheumatoid Arthritis, Deena Al-Samman, Nashwan Al-Asaady, Salem Al-Jader
This study was conducted to evaluate the response and safety of rituximabin combination with methotrexate with the aim of improving the quality of life in patients with active rheumatoid arthritis in Mosul city, Iraq.The study was done at Al-Salaam Teaching Hospital in Mosul City. Patients were well educated about the therapeutic profile of rituximab, including both risks and benefits. Enrolled patients: Male or female patients ≥ 18 years with active RA for at least 6 months before the study, inadequately responded to methotrexate therapy. The disease activity was assessed by measuring Disease Activity Score (DAS28). Indication of disease activity was swollen joint and tender joint count ≥ 8 and DAS28-ESR >5.1. Patients were excluded if: < 18 years or over 65 years, concomitant treatment with any DMARDs (other than methotrexate), chest infection, tuberculosis, hepatitis, diabetes, low immune patients, pregnant or breast feeding women. A detailed history and complete physical examination in all patients preceded rituximab therapy. The treatment included rituximab(MabThera1000mg) infusion on days 1 and 15 in addition to methotrexate. Patients were assessed at baseline, at the follow-up at 2 and 12 weeks post treatment, for disease activity score and for the safety outcomes. Remission rate was defined as DAS-28 < 2.6, swollen joints< 4, tender joints< 2,pain score
180 Assessment Of Awareness About Foot Care Among Diabetic Patients With or Without Foot Ulcer, amani Mohamad A Al-Dalawi, Ameer Kadhim Al-Humairi
Assessment Of Awareness About Foot Care Among Diabetic Patients With or Without Foot Ulcer
181 Evaluation of postdural puncture headache after 12 hours of elective caesarian section by using 25G cutting and 25G non-cutting spinal needles: comparative study, Anas Amer Mohammad, Majeed Mohammad Mahmood
Background: headache is the most common complication that occurs due to puncturing of the dura in more than 36.5% of spinal taps, it occurs 48 hours after the procedure due to leakage of cerebrospinal fluid throughout puncture site of dura to epidural and paravertebral space, this headache may last for 1-2 days or even for 2 weeks. Methods: This cross sectional study was donebetween October 2016 and January 2017, the 100 successive women aged 18-35 years old in Duhok Maternity Hospital, underwent spinal anesthesia for elective cesarean section. They were randomly distributed into two groups, Group I: (50 females) 25G cutting spinal needle was used, Group II: (50 females) 25G non cutting spinal needle was used, the data collected 12 hours after surgery.The collector of data was double blind and used questionnaire in closed envelope. Results: The mean age of sample size was 28.39±4.01 years. The study showed that the total incidence of the headache among the patients was 10% with only mild severity. With respect to the headache criteria, the study showed that the headache of most of them occurred after mobilization (90%). The incidence of PDPH among the patients who underwent an anesthetic technique with 25G cutting needle was statistically significant higher compared to that with 25G non-cutting needle, 8%and2%respectively(p=0.046). Conclusion: the25G cutting spinal needles were responsible for a greater incidence of postdural puncture headache (PDPH) 12 hours postoperatively, in comparison with the 25G non-cutting (pencil-point tip).
182 Molecular effect MDR1(C3435T) gene polymorphism on leukemia patients in Babylon province, Iraq, Asmaa Mohammed Mekkey, Alyaa Saad Abed, Zainab Mohammed Jasim, Nawres Najah Jawad
Background: Acute leukemia is the causing death between many people. Many previous discussions have showed an correlation between MDR1 polymorphisms and leukemia risk. Objective: This study is used to show a strong significance between the genetic MDR1 polymorphism and leukemia in gene site (C3435T). Methods: A case control study involving 70 leukemia patient and 40 healthy. was conducted. Specimens of all patients and controls that carry MDR1.C3435T gene polymorphism were identified by conventional. PCR followed by RFLP. methods. MDR1 (C3435T), polymorphism, was. detected by PCR amplification by using the following primers: forward, primer 5’ TTG ATG GCA AAG AAA TAA AGC 3’ and .reverse primer 5’ CTT ACA TTA GGC AGT GAC TCG 3’. Results: Our results were significantly related with development of acute leukemia patients. C3435T allele. frequency widely varies between variants people. Conclusion: These polymorphisms are correlated with the advanced risk of acute leukemia.
183 Smooth Emergence from General Anesthesia by Deep Extubation with No Touch Maneuver regarding Coughing in Ophthalmic Surgery, Ibtesam Ghaneim Ali
Background: Deep tracheal extubation with no touch maneuver in anesthesiology scene perform by anesthesiologist while patient still anesthetized without stimulation related to tracheal tube oscillation, suctioning, or changing patient position at emergence time from general anesthesia. Aim of the study for applying this technique seeking smooth recovery by avoiding cough incidence in ophthalmic operations. Methods: In this Interventional study, 27 Patients of both genders with different age groups ranging from 2years to around 60 years undergoing assorted ophthalmic procedures were subjected in this study by applying deep extubation with no touch maneuver. The study included patients who attending Ophthalmic Surgical Department at Ghazi AL Hariri Hospital in Baghdad City, Iraq. The incidence of cough was recorded, and any event related to emergence state or a drug need to be given was noted. Results: The present study showed there is significant association with patient age where younger one precisely children got smooth recovery with no or slight cough. The smoker participants got no benefit and more aggressive recovery with deep extubation and no touch maneuver. Conclusions: The present study showed that emergence from anesthesia with applying deep extubation and without touch maneuveror stimulation let patient returns to conscious state smoothly without or with slight cough in ophthalmic procedures.
184 The satisfaction of patient with removable partial denture therapy from point of the comfort and chewing efficiency , Zainab Mahmood Al-Jammali, Azad Al-Muthaffer Anas Al-Yasiry, Zahra Saad
The aim of this study was to assess relation of satisfaction level of RPDs related to comfort and chewing efficiency of patients who seeking treatment of removable partial denture. Thirty two patients with a removable partial dentures, were examine, age range (30-60) years. After construction of the prosthesis, we ask the patients questions about comfort and chewing efficiency and the satisfaction of patient was evaluated by using scale range from 1 to 5.Results found that the largest percentage of the female were satisfy (55%) with the comfort of her denture compared with the male patients and the differences significant.The largest percentage recorded in the employed patient (52.6%) were satisfied compared with the non-employed patient who have (46.2%) were satisfied.About chewing efficiency, the largest percentage recorded for the male patient (50%) was satisfied compared with the female patients. Among age groups, the largest percentage was for the G.I satisfy compared with the G.II. The largest percentage recorded in employed patients compared with the non-employed patient.About comfort, the largest percentage of the female were satisfy of her denture compared with the male patients, the largest percentage for G.II was satisfy compared with the G.I, and the largest percentage recorded in the employed patient were satisfied compared with the non-employed patient. About chewing efficiency, the male patients were more satisfied than female patients, in relation with age, the largest percentage was for the G.I satisfy compared with the G.II, and the largest percentage recorded in employed patients were satisfied compared with the non-employed patients.
185 Determination of K, Na and Ca with flame atomic emission spectroscopy and screening of active compounds of Withania someniferum roots from Iraq, Nehad Kanaan Abed, Ali Rasool Mahmood Al-Bakaa, Mohammed Abdullah Ahmed, Zainab Abbas Jabbar
A soxhlet Carbon tetrachloride, Chloroform, Ethanol and Aqueous extracts from the W.S. roots (In Iraq as knowing sem alferahk), which traditionally used for treatment of infertility; Alkaline elements (K) potassium, (Na) sodium, and (Ca) calcium were calibrated by F.A.E.S., and screening of active compounds of W.S. roots, to analyze Q.P. of various Carbon tetrachloride, Chloroform, Ethanol and Aqueous extracts of root parts of W.S. The present study investigates the qualitative analysis of the major bioactive constituents of root parts of W.S. in some solvents (Carbon tetrachloride, Chloroform, Ethanol and Aqueous water); Phytochemical screening method was used to identify qualitative analysis of bioactive component. While the quantitative analysis of (K, Na, and Ca) was analyzed by F.A.E.S.; The extraction results show higher concentration of potassium then sodium; While calcium doesn’t exist. The bioactive component flavonoids were foundin all those extracts; but only CCl4 and CHCl3 extract contents saponin, while tannin and unthioqunone doesn’t exist in four extracts.
186 Rapid Typing of Methicillin Resistant Staphylococcus aureus Isolated from Intensive Care Units in Babylon, Iraq, Huda H. Al-Hasnawy, Inas Ahmed Saeed, Monqith A. Al-Janabi, Ali S. Baay, Zainab Hashim Nasser
A total of (96) lavage specimens were taken from patients with ventilator associated Pneumonia (VAP), and bronchioalviolar lavage (BAL), during the period from February to June (2018) admitted to the Al-Hillah General Teaching Hospital, Al- Imam Al-Sadiq Hospital and Tiba Center. Among them 24(25%) of specimens were found to be S. aureus isolates were recovered from lavage from patients with ventilator-associated pneumonia and bronchoalveolar lavage(BAL) from patients with bronchiectasis, 22 (36%), 2(5.7%) respectively. In this study, the (21) Methicillin- Resistant S. aureus MRSA isolates were subjected to susceptibility testing (DDT) according to the CLSI, (2016) guidelines using (13) different antibiotic disk. The resistance rate to the Cefoxitin, Oxacillin, Azithromycin, Ceftazidime, Tetracycline, Doxycycline, Amikacin, Gatifloxacin, Ciprofloxacin, Kanamycin, Trimethoprim-sulfamethoxazole, Rifampicin is 21(100%), 18(85.7%), 16(76.1%), 21(100%), 17(80.9%), 3(14.2%), 21(100%), 7 (33.3%), 1(4.7%), 15(71.4%), 6(28.5%), 2(9.5%) respectively. The resistance rate was low for each the following antibiotics: Ciprofloxacin, Rifampicin, Doxycycline, 1(4.7%), 2(9.5%), 3(14.2%) respectively, while the nonresistance rate (0.0%) to Vancomycin. Molecular identification of MRSA isolates by the gene mecA for (24) isolates, about 22(91.6%) isolates were confirmed for exist of mecA by PCR-technique. Genotyping of MRSA Staphylococcal Cassette Chromosome mec SCCmec typing by multiplex PCR-technique found the majority of the (24) MRSA isolates that investigated, were found to be belonged to SCCmec II (79.1%) followed by SCCmec III (33.3%), SCCmec IV (25%), SCCmec V (25%) and SCCmec I (8.3%).
187 A total of (96) lavage specimens were taken from patients with ventilator associated Pneumonia (VAP), and bronchioalviolar lavage (BAL), during the period from February to June (2018) admitted to the Al-Hillah General Teaching Hospital, Al- Imam Al-Sadiq Hospital and Tiba Center. Among them 24(25%) of specimens were found to be S. aureus isolates were recovered from lavage from patients with ventilator-associated pneumonia and bronchoalveolar lavage(BAL) from patients with bronchiectasis, 22 (36%), 2(5.7%) respectively. In this study, the (21) Methicillin- Resistant S. aureus MRSA isolates were subjected to susceptibility testing (DDT) according to the CLSI, (2016) guidelines using (13) different antibiotic disk. The resistance rate to the Cefoxitin, Oxacillin, Azithromycin, Ceftazidime, Tetracycline, Doxycycline, Amikacin, Gatifloxacin, Ciprofloxacin, Kanamycin, Trimethoprim-sulfamethoxazole, Rifampicin is 21(100%), 18(85.7%), 16(76.1%), 21(100%), 17(80.9%), 3(14.2%), 21(100%), 7 (33.3%), 1(4.7%), 15(71.4%), 6(28.5%), 2(9.5%) respectively. The resistance rate was low for each the following antibiotics: Ciprofloxacin, Rifampicin, Doxycycline, 1(4.7%), 2(9.5%), 3(14.2%) respectively, while the nonresistance rate (0.0%) to Vancomycin. Molecular identification of MRSA isolates by the gene mecA for (24) isolates, about 22(91.6%) isolates were confirmed for exist of mecA by PCR-technique. Genotyping of MRSA Staphylococcal Cassette Chromosome mec SCCmec typing by multiplex PCR-technique found the majority of the (24) MRSA isolates that investigated, were found to be belonged to SCCmec II (79.1%) followed by SCCmec III (33.3%), SCCmec IV (25%), SCCmec V (25%) and SCCmec I (8.3%)., Nadia Mudher S. Al-HillI
Background: Recurrent miscarriage has been investigated for a long time with different types of therapeutic trials for those with unknown cause. Prednisolone as an immune modulator agent can have beneficial effect in improving pregnancy outcome in those patients. Objective: The aim of this study was to investigate the role prednisolone in preventing is carriage in patients with recurrent pregnancy loss. Methods: Sixty two patients enrolled in this study, all of them have recurrent miscarriage, after investigations and exclusion of those with antiphspholipid syndrome, medical disease, endocrine disorder and uterine pathology. Patients divided randomly and equally into two groups. Study group give prednisolone therapy 5 mg orally for two months prior to conception & continue during pregnancy till 13 weeks of gestation, while the second group received folic acid 5mg orally before conception and continue till the end of first trimester. Patients were followed up throughout pregnancy till delivery.Results: There was significant association between pregnancy outcome and type of treatment majority (90.3%, n=28) of patients using prednisolone continue treatment, while only (38.7%, n=12) of control group continue their pregnancy. (X2=18.03, P=
188 Evaluation of malonaldehyde levels and study of its association with occupational stress in nurses, Israa Harjan Mohsen , Ehab Jasim Mohammed , Husain Ali Kareem, Ashraqat Amer, Doaa Ayad Jabber
The present study is aimed to study stress factors and its association with workplace violence against nurses. The study included 100 samples of nurses in the AL Imam AL-Sadiq Hospital in Babil Governorate-Iraq.The outcomes of the current researchdisplaythat the plurality of the participants were females (60%) and married (66%).The prevalent of nurses are worked in Emergency unit (62% ) females and (44%) males respectively,most of them were in the day shift and years of experience (< 5).The malonaldehyde level (MDA) is significantly different amongthe men and women at
189 Evaluation of 2D speckle strain in β-thalassemic patients of pediatric age group for early detection of left ventricular dysfunction, Zainab F. Hassan, Mohanad K. Shukur Alghanimi, Ahlam Kadhim Abbood
Background: Thalassemia is a disease caused by abnormal hemoglobin synthesis with a resulting of chronic anemia. Major cause of death is heart failure due to combination of anemia and excess iron from repeated transfusion.Objectives: To evaluate the role of 2D speckle strain in thalassemic patients in early detection of cardiac dysfunction.Methods: This is a case control study included 20 thalassemic patients with mean age (10 ±3.8 year), and compared with 20 healthy subjects as control group with (mean age 11± 1.7 year). They were enrolled from obstetric and pediatric hospital-thalassemic center in Babylon province. 2D speckle tracking strain was evaluated in both groups. Results: Segmental strain values were significantly lower at the basal anteroseptal LV (19.5 ± 3 vs. 22 ± 2.7; P = 0.01), basal posterior LV (-19 ± 5 vs. 22 ± 3.5; P = 0.05), mid anterior (22 ± 5 vs. 24± 2.3; P = 0.05) as compared to controls.Average longitudinal strain (ALS) were lower in patients with TM that receive blood in a period less than 15 days than in patients that receive blood in a period more than 15 days. However, these changes are not significant (P>0.05).Conclusions: From the early changes in segmental strain values, early detection of cardiac dysfunction could be achieved, and the intervals between blood transfusion also affects the development of cardiac dysfunction, the longer duration the less development of cardiac dysfunction
190 Different cytokines and Lipid Profile in Suicidal and Non Suicidal Adults with Major depression, Suaad Mohammed H. Rasheed, Ali J. Eidan, Arafat Hussein Al-dujaili, Layla Hadi Abada, Alaa H. Al-Charrakh
Background: Suicide is a substantial public health concern and one of the commonest cause of death throughout the world. In Iraq, the suicide rate is a current health problem exacerbated by the fact that it is a country suffered war and destruction in all fields of life. Objectives: We tested whether Tumor necrosis factor-alpha, Interlukin-1β, and lipid profile might be associated with suicide attempts in adult patients with major depression. Methods: Plasma levels of Tumor Necrosis Factor-alpha, Interleukin-1β, and lipid profile were analyzed in 60 adult patients with major depression diagnosed according to DSM-V criteria for Major depression (22 suicidal attempters and 38 patients without suicidal ideas), and 30 healthy controls. Results: At univariate analyses, plasma level of Tumor Necrosis Factor-alpha and Triglyceride were significantly higher in suicide attempters and non-suicidal attempter depressed patients than those of healthy control (all p
191 Quantification of Biofilm Production and Molecular Identification For Highly Yielding Production Isolates , Noor Abd-Ulamer Oda, Suhad Redha Al-Tayie
Background: Biofilms are aggregation extracellular matrix produce by some bacteria considered important for the existence of it, in inadequate environmental conditions, which are organized into structural communities and subpopulations if produce provide exo – polysaccharide (EPS) matrix as a major component structure for their stability. Objectives: In the present study, we investigated biofilm forming ability in two different media beside the molecular identification for high yield biofilm production isolates. Methods: In this study a 32 isolates obtained from Central Public Health Laboratory in Karbala City. The ability for biofilm production were evaluated and compared in two distinct media (Luria Bertani- LB broth and Tryptic soy broth - TSB). Microtitre plate (MTP) assay was used to quantify the biofilm production ability from bacterial isolates. DNA was extracted, molecular identification was accomplished by fragmentary Sequences of 16s rRNA gene in nine more effective biofilm production isolates. Results: From the total number of isolates 12.5% could be categorized as a weakly biofilm former ; 62.5% moderate and 25% strongly produced to biofilm in LB broth media, whereas (37.5%, 53.1% and 9.4% ) were weakly; moderately and strongly produced to biofilm respectively in TSB media . Molecular identification of the selected isolates showed S.1 had 99% similarity to Bhargavaea cecembebsis ;S.2 had 100% similarity to Staphylococcus aureus; S.3 had 99% similarity to Bacillus licheniformis; S.4 had 99% similarity to Bacillus sonorensis; S.5 had 100% similarity to Stenotrophomona maltophilia and S.6 had 100% similarity to Enterococcus faecalis; while no PCR amplification results were given in S.7, S.8 and S.9 isolates. Conclusion: All of isolates in present study had biofilm forming ability, 9 isolates can be categorized as strong biofilm former in LB broth media, while 3 isolates can be categorized as strong biofilm former in TSB broth media. Also, the ability of biofilm production in LB media was higher than when TSB media was used (p= >0.0001). The appropriate medium for studying and detection the ability of bacteria to the production of biofilm varies according to the type of bacteria as it has been shown Tryptic soy was more suitable of Bacillus sonorensis for detection biofilm formation as compared to the Luria Bertani broth in the present investigation.
192 Effects of antidiabetic drug on the anticancer activity of Cisplatin, Ahmed Ibrahim Rashid , Kaiser N. Madlum, Rana Ghaleb, Hamid Naji Obied , Sabah H. Enayah
Although its mechanism of action on cancer cells not well understood, Metformin (Met) is widely used nowadays to improve the anticancer activity of some drugs. Metformin has also been shown to decrease the growth of breast cancer cells and pancreatic cancer in hamsters and delays other types of tumors. The aim of this study was to compare the effects of Cisplatin (Cis) alone and Metformin alone on colon cancer cell line SW480. The results showed that the effect of Metformin on cell proliferation is concentration-dependent. Metformin enhances the proliferation and attachment of colon cancer at lower concentrations. Results showed a significant decrease in cell proliferation and attachment of colon cancer cells after Cisplatin treatment.Results of this study revealed that Cisplatin treatment decreased both proliferation and cell adhesion to the matrix. Combination therapy (Met+Cis) showed promising synergism and enhancement of anticancer activity of Cisplatin on colon cancer cells. We are strongly recommended more investigations to be more specific for using Metformin as general anticancer.
193 Cytotoxic Effects of Bardoxolone on HCT-116 Human Colonic Cancer Cell Line, Naiel A. Kadhiem, Sameer H. Al-Rikabi, Talib H. Kammona, Hamid N. Obied
Background: Nuclear factor-κB (NF-κB) transcription factors comprise a key role in many physiological processes such as innate and adaptive immune responses, proliferation of cells, cell death, and inflammations. Anti-NF-κB therapy may rescue different cases of colonic carcinoma and would be considered as a therapeutic goal. The aim of the study is to detect whether Bardoxolone is effective in treatment of colonic carcinoma by apoptosis and regression of tumor markers and activation of tumor suppressor genes as compared to other FDA approved anticancer agents as 5-FU. colonic cancer cell line (HCT 116). These cells are cultured in vitro according to routine cell culture protocols using specific media and reagents. Methods: Treatment groups are classified into 4 groups (control, Chemotherapy group, Bardoxolone and combination treatment groups).Results: The results revealed significant increase in inhibition concentrations IC50 of colonic cancer cells in low concentrations of bardoxolone treatment group and in combinations treatment group as compared to control group (P< 0.05). Also, the inhibition percent of different concentrations of 5-FU and Leucovorin on HCT-116 colonic cell line after 24h of incubation were significant (p< 0.05) as compared to control group(cancer cells without treatment) in all concentrations (2.5, 5, 10, 20, 40, 80 µgm/ml). Conclusion: Bardoxolone has a significant anticancer and cytotoxic effect in low micro-concentrations on human colonic cancer cells as compared to control groups.
194 Disorders of sex Hormones and lipid profile in obese and non-obese Women with Polycystic Ovary Syndrome (PCOS) in Karbala City, Hameedah Hadi Abdulwahid, Batool A.Hussein, Zahraa Sabbar Omran, Saleem A. Alhasanawy
Background: Infertility is the inability to become pregnant after one year of intercourse without contraception. Polycystic ovary syndrome (PCOS) is the disorder of reproductive age women. Objectives: to determine FSH, LH, testosterone hormone and prolactin levels, cholesterol, triglyceride, HDL, and LDL concentrations in infertile women. Methods: This study was carried out in department of women and obstetrics in Al-Kafeel hospital and Women maternity teaching hospital in Karbala city. A Total of 100 samples divided into (2 groups) group 1 is a poly cystic ovaries syndrome PCOS group (50 patients) who diagnosed on the basic collection of two out of three features (ultrasound features, clinical features, laboratory features. The control group included 50 samples with on the symptoms in this group. Results: Results showed a significant difference of serum level LH (p=0.001) and testosterone level. (p=0.010) between patients and controls group. When study lipids profile (CHOL, TG, LDL, HDL) in PCOS patients and control. The result shows no significant between PCOS patients and control. When correlation between serum level of sex hormone and lipids profile in non-obese female in patients group, In non-obese women there was a significant positive correlation between (CHOL) and testosterone. Prolactin and testosterone, Prolactin and TSH, Testosterone and TSH. There was a significant negative correlation between TG and FSH. Correlation between serum level of sex hormone and lipids profile in obese female in patients group, there was a significant positive correlation between Prolactin in and LH, TG and TSH Conclusion: The relationship between cholesterol and the testosterone hormone in thin women, as well as the relationship of prolactin with test. and TSH, obese women, showed the relation of prolactin and LH and TG with TSH. For this reason, we recommend that women maintain the level of lipid profile for The body, whether obese or skinny.
195 Comparison of sandwich, conventional antagonist and microdose protocols in poor responders, Milal Muhammad Al-Jeborry
Background: Poor response to controlled stimulation is a major problem in ART. Increasing number of mature oocytes and embryos is an important aspect for successful outcome. Objectives: To assess the efficacy and outcome of pregnancy of poor responder women underwent ICSI program using sandwich, conventional antagonist and micro dose protocols. Methods: The randomized controlled trial study included 158 poor responder women underwent ICSI program diagnosed according to POSEIDON classification, were randomly divided into three groups; Microdose protocol N=41, conventional antagonist protocol N=71 and sandwich protocol N=46.The program performed by using high dose gonadotrophins from second day of cycle till administration of HCG. The primary outcomes were number of retrieved oocytes and pregnancy rates. Secondary outcomes were duration of stimulation, total amount of gonadotrophins needed, endometrial thickness on day of HCG trigger, maturity of oocytes, number of transferred embryos, fertilization rates, implantation rates and cancellation rates. Results: There was higher endometrial thickness on day of administration of HCG in microdose group compared to sandwich and conventional antagonist groups (p˂0.05%).While there were higher numbers of retrieved oocytes, mature oocytes, number of transferred embryos with higher fertilization rate, implantation rate and lower cancellation rates in sandwich group compared to microdose and conventional antagonist groups (p˂0.05%). While the pregnancy rate was slightly higher in microdose than sandwich group. While there was no statically significant difference regarding stimulation period, total required amount of gonadotrophins and peak estradiol level on day of HCG trigger (p-value >0.05%. Conclusions in poor responders the sandwich protocol improved the outcome of pregnancy.
196 Effect of polymorphism on CTLA-4 gene on Graves' Disease, Eman Th. Al-Fatlawy, Israa K. Al-Yasiri, Ibtihal Al-Shamarti
Background: Graves is one of autoimmune Thyroid Diseases. Moreover, more than 20 genes polymorphisms are associated with Graves’s diseases. Although, the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) genes play an important role in immunity system function, strategies to enhance CTL4 polymorphism gene may be impact on increase Graves' disease. Recent research has revealed that the 49A/G polymorphism in exon 1 has a detrimental effect on this disease, results in a threonine-to-alanine conversion. Objectives: The study was carried out to investigate and affect allele polymorphism CTL4 gene in Graves' disease in AL-Najaf province. Methods: In our study, 73(48 females and 25 males) individuals who detected Autoimmune hyperthyroidism and twenty healthy as a control groups. All Graves patients were recruited from the hormonal unite at Al-Sadder Medical City, Najaf/Iraq. The control group participating in the study did not have any history of immune diseases and chronic diseases. The study was approved by the ethical committees of Al-Sadder Medical City. Genomic DNA was extracted then polymerase chain reaction fragment length polymorphism (PCR-RFLP) technique is used, follow by Sequence data of polymorphism. Results: Results of this study found that all samples were digested with restriction enzyme Fnu4HI in two fragments (99 and 63) bp. These results indicate that 49 A/G polymorphism GLT4 gene was detected in Graves patients and control. Conclusion: Contrary to expectations, this study did not find a significant difference between Graves patients and healthy individuals in the Najaf city population.
197 Frequency of human hemochromatosis HFE gene mutations and serum Hepcidin Level in iron overload β-thalassaemia Iraqi patients, Naeem Mohammed Mohsen Al-Abedy, Ehab Dawood Salman, Safa A Faraj
Background: Thalassemia is an important hematological disorder. Iron overload is the main cause of morbidity and mortality in patients with β-thalassemia. The possibility of iron overload development may be increased by Interaction between thalassemia and HFE gene mutations. This study aims to investigate the possible association between serum hepcidin level as an indicator of iron concentration and the presence of HFE gene mutations or the effects of these gene's polymorphisms on blood hepcidin level and iron overload in the beta-thalassemia patients. Methods: A eighty two of β- thalassaemia (56 BTM and 26 BTI) patients and fifty normal controls were included in this study. Hepcidin levels along with iron parameter were determined. We assessed the frequency of the H63D, C282Y and S65C mutation of the HFE gene and the correlation between these mutations and the iron overload in β-thalassemia patients. Genotyping of H63D, C282Y, and S65C of HFE variants performed by T-Plex real-time PCR.. Results: Serum hepcidin level was found to be reduced in BTI and BTM patients as compared to controls .Hepcidin level is significantly lower in BTM compared to control (P
198 The Role of free radicals in female unexplained subfertility, Ban Jaber Edan, Huda M. Shakir, Naseer J Almukhtar
Background: Unexplained sub-fertility is commonly identified if couples fail to conceive after 1 yr of everyday unprotected sexual intercourse even though investigations for ovulation, tubal patency and semen evaluation are ordinary. For as many as (30–40%) of couples experiencing sub-fertility, their sub-fertility stays unexplained. The objective of current study was to estimate and calculate the reactive oxygen species and to determine the relationship between free radicals, antioxidant enzyme activities and unexplained subfertility. Methods: The study was an observational prospective study involved two groups of women who attended Babylon maternity Hospital and private clinic. The study groups included 30 apparently healthy fertile women as a control group and 60 women with subfertility. Blood withdrawn from both groups for biochemical evaluation of MDA, GSH, vitamin E and vitamin C. Cervical secretions were also collected from both groups for same enzymatic and non-enzymatic free radicals evaluations. Results: The results showed that MAD levels were increased significantly (P
199 The Differences between Benign Mixed Tumor and Papillary Cystadenoma Lymphomatosum in Proliferative, Apoptotic and Antiapoptotic Activities, Ameera K Khaleel
Background: The majority of salivary glands tumors are benign mixed tumors. The present study was aimed to evaluate the differences between benign mixed salivary glands tumor and papillary cystadenoma lymphomatosum regarding the tumor cell proliferation rate, apoptotic and antiapoptotic activities. Methods: The study was performed on archived paraffin-embedded salivary glands tissue of 23 benign mixed tumor and five cystadenoma lymphomatosum. Sections were stained with hematoxylin and eosin and cases with definite diagnosis were selected for immunohistochemistry. The immunoreactivity was assessed in areas of highest positivity regarding Ki-67, P53 and bcl-2. Results: Benign mixed tumor and papillary cystadenoma lymphomatosum showed negative immune expression for Ki-67. The P53 immune staining in benign mixed tumor appeared negative while the papillary cystadenoma lymphomatosum appeared with a mild positive staining. The bcl-2 immune expression in benign mixed tumor was moderately positive while the papillary cystadenoma lymphomatosum was mildly positive. Conclusion: The benign mixed tumors showed significantly more Ki-67 and bcl-2 immune labeling indexes than papillary cystadenoma lymphomatosum, but the P53 immune expression was less (P
200 A correlation between sonographic and histopathological findings of gallbladder polyps, Firas A Noori, Alla J Hasin
Gallbladder polyps have become a common ultrasonographic finding. The management of these polyps is complex since they can carry malignant lesions. Our study had aimed to analyze the results of ultrasound and pathological findings of patients operated upon due to polyps in the gallbladder. The study includes patients with ultrasound diagnosis of gallbladder polyps who underwent cholecystectomy in 2017-2019 were reviewed, and demographic, sonographic and histopathological data were collected. Sixty-eight patients were involved in the study. The median age was 42 ± 7 years, and 63.2% of our patients were women. The average size of the ultrasound polyps was 6.8 ± 4 mm. Histopathology confirmed the presence of polyps in 95.6% of patients, with an average size and number of lesions of 7.5 ± 5.8 mm and 1.7 ± 1.2, respectively. Eight polyps were larger than 10 mm, and the individual polyps were significantly larger than the multiple ones (p = 0. 004). Three cases of adenoma were diagnosed (4.6%); one of them was cancer in situ. All were single and over 10 mm. We found an important correlation between determining the size of an ultrasound polyp and the pathological anatomy (r = 0.93; p = 0.002). The tumor size was an indication of the presence of adenoma (p = 0.009, (95% CI = 1,113-1,678). We have concluded that there is a clear correlation between the size of the gallbladder tumor on ultrasound and the size in the pathological report. Gallbladder adenoma is uncommon and it correlates with the size of the polyp. In this study, the size was the only indicator of the presence of adenoma.
201 Incidence of early complications in laparoscopic total thyroidectomy vs. open thyroidectomy using breast approach in simple multinodular goiter., Alaa J Hasin, Kasim Traim, Muslim Kandel
Total thyroidectomy is a popular surgical operation around the world. It can be performed through collar incision which may end with a bad cosmetic appearance for this type of wound in the neck. Recently, laparoscopic total thyroidectomy has become popular in experience laparoscopic hand surgeons. There are many early complications that may occur after laparoscopic total thyroidectomy due to a new technique in total removal of the thyroid gland to give excellent cosmetic results. In our study, we compared the incidence of early complications, which occur within early 30 postoperative days, between LTT (laparoscopic total thyroidectomy) and OTT (open total thyroidectomy). 122 patients who had total thyroidectomy operation for simple multinodular goiter, 58 patients underwent LTT, while 64 patients OTT in GIT center in Thi-Qar city. All the operations were done by the same surgeon for all patients. The study was done through 2 years, from 1/5/2017 till 30/4/2019, through breast approach and collar incision. The surgical outcome has recorded the complications that occur to the patients within 30 days post-operatively.
202 Assessment of serum zinc, copper and copper/zinc ratio in adult patients with psoriasis, Nazer HJ Al-Sultany, Ban MS Al-Joda, Mohammed K Al-Hattab
Background: Psoriasis is an immune-mediated, chronic inflammatory disease of genetic basis, which affects mainly the skin, although it has systemic pathological effects. The most severe forms have been associated with several diseases that have similar pathogenic factors. Many studies showed that trace elements play vital role in some inflammatory responses such as psoriasis. The aim of current study was to estimate the levels of serum trace elements (zinc, copper and Cu/ Zn ratio) in psoriatic patients and evaluate the impact of these values on the severity and pathogenesis of psoriasis. Methods: In this case-control study, (50) patients with psoriasis were divided into three subgroups (mild, moderate and severe) and (50) healthy controls were examined. PASI score for each patient was calculated. Serum levels of Cu and Zn were measured by Flameless Atomic Absorption Spectrophotometer and Cu/ Zn ratio was calculated. Results: After measurements and comparisons between psoriatic patients and age-, and gender-, matched control group, results showed significant differences in copper and Cu/ Zn ratio among these groups, but no significant differences in serum zinc were showed. There was significant increase in serum copper and Cu/ Zn ratio (P value = 0.017 and 0.032 for copper and Cu/ Zn ratio, respectively) for psoriatic patients as compared with control group. Results also showed no significant differences in serum zinc between psoriatic patients and control group. No significant differences in the concentration of serum copper were observed between females and males of patients group, but highly significant increase of serum zinc in female as compared with males of psoriatic patients. Conclusion: increased serum copper may play a major role in increasing the inflammatory response in psoriatic disease. No apparent relationship was observed in our study between psoriasis pathogenesis or severity and level of serum zinc. In addition, Cu/ Zn ratio is a good marker for measuring severity of psoriasis.
203 Study of s-adenosylhomocysteine as marker of Acute Myocardial Infarction in Thi-Qar heart center, Lamees M Al-Janabi, Dheyaa K Al-Waeli
In the current study, we investigated the correlation between serum s-adenosylhomocysteine as a new marker for myocardial infarction severity with homocysteine, troponin, vitamin B6 and B 12 on 90 acute myocardial infarction patients and 120 normal persons as control.The data of all patients and controls were measured by using the ELISA technique except troponin was measured by using the VIDIS technique, while the data were analyzed by SPSS software. The results of this study show a significant increase in s-adenosylhomocysteine, troponin, homocysteine, while vitamin B6 and B12 were decreased significantly.The correlation study proved that s-adenosylhomocysteine as a golden marker for AMI patient for male and female-specific with SEAMI while troponin with high correlation in NSEMI.
204 Histological changes and hyperlipidemia criteria for triton and therapeutic efficacy of Punica granatum husk extract in liver tissue of male white rabbits, Ansam H Ali, Ahmed H Ahmed, Zinah I Khaleel
Background: Triton is a non-ionic surfactant that acts to raise or increase intestinal absorption of fat through its emulsification process. It changes the permeability of cell membranes and thus causes cell lysis and increased susceptibility to infection. Antioxidant effects of pomegranate studies have shown that pomegranate seed extract contains compounds with an antioxidant capacity of 2 to 3 times more than green tea. The present study aimed to study the effect of triton on the histological structure of white rabbit liver and to try to get rid of the effects of triton used alcoholic extract pomegranate husks. Methods: Observational and behavioral symptoms were observed in triton-treated animals, such as lack of water and food intake, inactivity in the movement and tremor with diarrhea. Results: The results of the study in the second group (G2), only injected with triton under the peritoneum at a concentration of 0.1mg/kg, showed the histological changes caused by triton in hepatic tissue with negative changes in tissue represented by degeneration of the majority of hepatocytes. In other regions, hepatic cell size swelling, cytoplasm and Kupffer cell numbers were observed, while the inhibitory effect and cellular repair of the histological structure of the studied organ were observed in the (G3) group, injected with triton under the peritoneum and dosed with pomegranate extract at a concentration of 1mg/kg, while no changes in tissue structure were shown. The liver in the fourth group (G4), dosed with pomegranate extract only at a concentration of 1mg/kg, according to the studied concentration compared to the control group (G1). Conclusion: From the results of the present study we can say that the extract of pomegranate husks has an effect in eliminating the effect of Triton as well as in cellular repair of liver tissue. .
205 Effect of Iron Therapy on Breath Holding Spells in Children Under Five Years of Age, Abdul-Kareem M Ali, Ahmed MA Abdulhadi, Salam A Tagi
Background: Breath holding spells are a common and dramatic form of syncope and anoxic seizures in infancy. They are usually triggered by an emotional stimuli or minor trauma. Based on color change, they are classified into 3 types, cyanotic, pallid and mixed. The aim of current study was to study the demographic profile of children with breath holding spells and iron deficiency and to assess the effect of iron therapy on reducing the frequency of breath holding spells. Methods: A clinical trial conducted in pediatric department at Al- Imamain Al-Kadhimain Teaching Hospital from 1st, April 2016 to 31st, March 2017. Fifty four children under five years of age with breath holding spells were screened out for iron deficiency anemia. A specially designed questionnaire was used to collect information including: name, age, gender, address and phone number for following up cases, onset of spells, frequency of spells, type of spells, family history, etc. Investigations included complete blood picture, blood film, serum iron, serum ferritin, total iron binding capacity were taken before and after completion of treatment. Children having breath holding spells were treated with oral iron supplement for three months. Results: Out of fifty four patients, fifty patients were included in this study and four patients with normal iron profile were excluded. Age of onset of breath holding spells, in 24 subjects, was 6-12 months, in 22 subjects was >12–24 months and, in 4 subjects, was >24-60 months. The mean age was 14.06 month. Male to female ratio was 1.7:1. The family history was positive in 36% of them. According to type of attack, 96% had cyanotic and 4% pallid attacks. In addition, 56% of patients presented with iron deficiency anemia and 44% had iron deficiency without anemia. Serum ferritin, serum iron and total iron binding capacity showed significant differences (P
206 Oxidative Stress Role in Aborted Women with Cytomegalovirus infection in Thi-Qar Governorate,Iraq, Alaa HA Al Nasir
Background: termination of pregnancy resulting in expulsion of an immature fetus of less than twenty weeks called as abortion. Where aborted fetus is weighing less than 500 g. Human cytomegalovirus infections is one of several factors related to spontaneous abortion. Aim: To determine the levels of oxidative stress (malondialdehyde) (MDA), (ceruloplasmin, transferrin, Vitamin C) (Cp,Tf,Vit.C) in aborted women with cytomegalovirus(IgG, IgG positive). Material and method: A case control study include (60) women aborted with CMV (Patients), (60) healthy women (positive controls) and (60) women aborted without CMV (negative controls). at the period between(November, 2017) to (May, 2018). Spss version 23 had been used for data analysis, P value
207 The effect of using cross training exercises on some Anaerobic abilities, EnzymaticAntioxidants, and lactic acid for handball players., Baba MT Al-Musawi, Noor N Hafed
The present study aims at: Preparing cross-training exercises. Identifying the effects of using cross-training exercises on some anaerobic and enzymatic antioxidants and lactic acid for handball players. The experimental approach whose pre -post tests was used as it fits the nature of the present study. The study is conducted on (14) players of Wasit handball team. Six (6) players were deliberately chosen to represent the research sample.The tests used in the present study are: Measuring lactic acid in blood. Measuring the variables; (GSH-MDA) in a (5cc) vein blood. Performing (defensive movement, launching for striking in a curve, speed, and hitting) tests. The results showed a significant development in the concentration of lactic acid in the post tests,which indicates the physiological effectiveness of the exercises. (GSH, MDA) significantly varied, which indicates the improvement of the functions of these enzymes as a result of the exercises. The level of anaerobic abilities; (defensive movement, launching for striking in a curve, speed, and hitting) tests improved as a result of the exercises.
208 Evaluation of Carbon Dioxide (CO2) Laser Incision Characteristics in A Viable Human Skin , Nibras AA Hindy, Suha A Hindy, Ahmed Hindy
Throughout the last decades, cutanous laser surgery has gained popularity among surgeons.This kind of surgery based on the principles of selective photo-thermolysis theory. CO2 laser selectively target water-containing tissue, applying controlled tissue vaporization. The tissue ablation and the zone of residual thermal damage is accompanied with a deeper tissue coagulation. A piece of viable intact human skin was taken. Multiple CO2 laser incisions were carried out with different laser parameters prior to be totally excised, then a histopathological examination were done for these skin samples. Slides were examined under light microscope to evaluate thermal enjury zones, wound edges, and cutanous blood vessles status. Histopathological examination showed that the three thermal zones were chenged wherever the laser parameter changed.
209 Creation with Validation of Reverse Phase- High Performance Liquid chromatography Assay (Content) Method for Sorbic Acid in Oral Solutions, Imad M M Al-Rubay
Sorbic acid is one of the antimicrobial agent used as an antibacterial and antifungal, which is used as preservative in food, pharmaceutical formulations (oral solutions and external products) and in cosmetic products to prevent mold, yeast, fungi and bacterial growth. Creation (Development or Foundation or Establishment) of RP-HPLC method for the determination of sorbic acid in oral solutions. The standard and sample were extracted withthe solvent mixture methanol and glacial acetic acid (95:5).The chromatographic condition: Column: L1,(25 cm × 4.6 mm),5μm,Detector Wavelength : 240 nm, Flow Rate: 1 ml/min, Injection Volume was 20 μl. The validation of the created RP-HPLC method was done according to USP, WHO and ICH guidelines with respect to Specificity, Linearity, Accuracy, Precision, Range, Limit of detection (LOD) and limit of quantitation (LOQ), System Suitability Determination and Robustness. The validation acceptance criteria were met in all cases.
210 Correlation of Prolactin and Thyroid Hormone Levels of Infertile Women, Athar H Abdullah, Ammar K Jaafar, Nady G AbdulKareem, Wildan T Mahmood, Muzahim Alkabban
Hyperprolactinemia is the most prevalent endocrine disorder in hypothalamic- pituitary axis especially among reproductive age female,affecting about one-third of infertile women.The prevalence of hyperprolactinemia in hypothyroidic patients is consideredas a cause for reproduction disorder (infertility) andfor early diagnosis and treatment of this problem . Sixty infertilefemale patients with primary hypothyroidismand sixty age matched (35-42 years old)apparently healthy control group, were selected from outpatients clinic of the specialized center for endocrinology and diabetes. Thyroide hormones (total triiodothyroninTT3 and tetraiodothyroninTT4), thyroid stimulating hormone (TSH) and prolactin were measured by electrochemiluminescent immunoassaymethod using automated analyzer and Vidus. In this study both serum TT3 and TT4 levels are significantly decreased (p
211 Glutamic Acid Decarboxylase-65 and Indices of Insulin Resistance inLatent Autoimmune Diabetic Patients, Mohammed J Kadhim, Nijoud F Yousif
Autoimmune diabetes is a heterogeneous disease which can arise at any age. Subjects with adult-onset autoimmune diabetes who do not necessitate insulin-therapy for at least 6 months after diagnosis are defined as having latent autoimmune diabetes in adults.The current work aimed to evaluate cytoplasmic islet cell autoantibodies and glutamic acid decarboxylase-65 in diabetic patients and their roles in insulin resistance. This study was conducted during the period from the end of December 2018 until the end of May 2019. A total of 60 patients were added to 30 healthy individuals for comparison. All patientsreviewing Center for Endocrinology and Diabetes at Al-Kindy Hospital. Some tests were conducted at the center, others at Al-Qasim privet laboratory in Al-Sadr City, and the Health and Medical Technical College-Baghdad and the Office of Scientific Progress in Al-Harithiya. There was a substantial rise in fasting serum glucose, glycated hemoglobin, cytoplasmic islet cell autoantibodies, and glutamic acid decarboxylase-65 while a significant decrease was found in serum insulin and c-peptide in diabetic patient as paralleled to the controls. Additionally, diabetic patients had low levels of triacylglycerol and high density lipoprotein cholesterol. It was found in this study that latent autoimmune diabetes in adults’ subset of diabetes exhibited metabolic features consistent with bothdefective insulin secretion and inslin resistance. They were found to be lean with low triacylglycerol and high density lipoprotein cholesterol levels.
212 A study for Malondialdehyde and lipid profile levels in ischemic stroke patients with and without diabetes mellitus, Majid A Maatook, Dheyaa S Hammad
The current study has focused on the levels of Malondialdehyde as lipid peroxidation marker and lipid profile in ischemic stroke patients with and without diabetes. The study was conducted on 118 subjects, 32 ischemic stroke patients with diabetes, 36 ischemic stroke patients without diabetes, and 50 healthy subjects as normal control. Serum Malondialdehyde (MDA), total cholesterol (TC), High-density lipoprotein cholesterol (HDL-C), Low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein (VLDL) and Triglyceride (TG) were assessed in all three groups. The diabetic and non-diabetic ischemic stroke patients demonstrated a significant increase of MDA, LDL, VLDL, TG, and total cholesterol when compared with control subjects, but with more increases in diabetic ischemic stroke patients. The study has also revealed that there is a significant decrease in the activity of HDL in both patient groups when compared with the healthy control group, but with less decrease in the HDL in the diabetic ischemic stroke group. We conclude that the assessment of MDA and lipid profile may be useful in early detection and monitoring diabetic patients with an increased risk of ischemic stroke
213 Effect of isolated obesity on systolic and diastolic left ventricular function , Talib A Salman
Background: Although "overweight" refers to an excess of body weight and "obesity" to an excess of fat, these two words can be defined in terms of body mass index. The aim of current study was to determine the direct effects of different grades of isolated obesity on echocardiographic indices of systolic and diastolic left ventricular function. Methods: This cross section study on 73 cases of isolated obesity who attended to the department of echocardiography at the AL-Kadhimiya Teaching Hospital. The information was collected using a questionnaire that was filled down through direct interview with patients. In current study, 48 obese and 25 normal weight persons were studied. They had no other pathological conditions .Obesity was classed as slight (body mass index (BMI) 25-29.9kg/m2; n= 17), moderate (BMI 30-34.9 kg/m2; n= 20) or severe (BMI >35kg/m2; n= 11). Echocardiographic indices of systolic and diastolic functions were obtained and dysfunction was assumed when at least two values differed by>2 SD from the normal weight group. Results: Ejection fraction and fractional shortening left ventricular dimensions were increased, but relative wall thickness was unchanged. No obese persons met criteria for systolic dysfunction. In obese subjects, the mitral valve pressure half time and the left atrial diameter were increased; whereas the deceleration slop was decreased, all other diastolic variables were unchanged. No differences were found between obesity subgroups. Conclusions: In persons with isolated obesity, subclinical left ventricular diastolic dysfunction is present in all grades obesity and is associated with an increased systolic function in the early stages.
214 Genomic Fingerprinting of Interleukin IL-12RB2 of Nasal Mucosal Stem Cells in Nasal Polyposes (NPs) of Iraqi Patients, Hatem A Hatem, Ahmed Muhei, Shayman J Ahmed, Nawfal K Yas
The inflammatory reactions cause nasal polyposes (NPs), which contained the paranasal sinuses and the nasal mucous membrane. They consist of recurrent multiple masses originating in the paranasal sinuses then spread from the middle meatus to the nasals cavity, which leads to the nasal blockage that causes the restriction of airflow to the olfactory area. This study aims at clarifying the role of IL-12RB2 polymorphism by using PCR technology in nasal mucosal stem cells in nasal polyps of Iraqi patients and use it as a biomarker. Fifty-eight cases of this study are referred to as nasal surgery, which selected from Dept.of Otolaryngology, Baghdad City, Iraq from May 2013 to January 2014. They were grouped into Control group (022 samples contained mucosa of inferior concha that gotten from patients subjected to septorhinoplasty intended for septal deviation) and study group (036 samples contained nasals polyps gotten from cases subjected to the surgical operation of nasal polypectomy). Gene aid; USA) kit was used for DNA extraction and confirmation of DNA extraction was done by using gel electrophoresis of PCR products. To conclude, the Data from the current study showed that PCR for IL-12RB2 was positive in the group of nasal polyps, but it was negative in control samples. In addition, Pearson Chi-squared statistics revealed a significant relation of PCR results when matched with pathological groups.
215 Changes of Salivary Interleukine-17, Apelin, Omentin and Vaspin Levels in Normal Subjects and Diabetic Patients with Chronic periodontitis, Entedhar R Sarhat, Zaidoon J Rmaid,, Talal H Jabir
Background: Chronic periodontitis is a chronic inflammatory disease which is multifactorial. Diabetes mellitus is one of the major systemic factors to influence the severity of chronic periodontitis. Therefore, the aim of current study was to assess salivary biomarkers of Interleukine-17(IL-17), apelin, omentin and vaspin as putative candidates in the potential association between Diabetes mellitus and Chronic periodontitis. Methods: Whole saliva samples were collected from sixty adults sex and age-matched patients who were further divided into two groups; healthy (control group; n = 30) and diabetic individuals with chronic periodontitis (n = 30). Salivary IL-17, apelin, omentin andvaspin concentrations were determined by standard enzyme-linked immune-sorbent assay. Results: Salivary levels of IL-17, apelin and vaspin were significantly higher in diabetic patients with periodontitis group compared to control group. On the other hand, salivary levels of omentin were significantly lower in diabetic patients with periodontitis compared to control group. Conclusions: Our study supports the hypothesis that abnormal production of IL-17, apelin, omentin and vaspin can contribute to the pathogenesis of diabetes-related complications including Chronic periodontitis.
216 Prevalence and determination of external and intraocular infections in Baghdad city, Zena K Khalil, Iman K Dewan, Saif S Al-Kamil
Background: Ocular infections conceded as one of the most commonly encountered infections with the more frequently affected areas are the conjunctiva, cornea and the eyelids. Bacterial conjunctivitis may begin in one eye, and then spread to the other one. This study was aimed to determine the prevalence and analyze ocular isolates that are obtained from patients with suspected ocular infections and their susceptibility to the most common antibiotics. Methods: The study included 314 patients attended to Ibn Al-Haitham hospital of ophthalmology in Baghdad. Their age ranged from 15 to 78 years. The study extended from May to November2018. Results: There were 240(76%) patients who had culture-positive bacteria, patients with extra ocular infections were 167(70%) and patients with intraocular infections were 73(30%). The results revealed that the number of patients with extra ocular infections were 91(59%) and 54(63%) with intraocular infections from Baghdad. The highest ratio of patients with external and intraocular infections belonged to the age group (70±0.21) years while the lowest ratio belonged to the age group (22±0.3) years. The results also found that 68(57%) of the patients were with extra and 55(45%) with intraocular infections had history of diabetes mellitus while 34(29%) of patients with intraocular infections had arthritis. The organisms cultured from the ocular surfaces (cornea, conjunctiva) accounted for the majority of 175(73%) of cases followed by the intraocular (aqueous humor and vitreous fluid) which were 65(27%). The highest rate was found in conjunctiva 155(65%) followed by vitreous fluid 50(21%) then cornea 20(8%) and aqueous humor 15(6%). Gram-positive bacteria were the most prominent pathogens for external and intraocular infections 147(61.25%). The most common species for external ocular infections were S. epidermidis 23(10%), P. aeruginosa 21(8.5%) and B. cepacia 20(8.3%) while the common species for the intraocular infections were S. epidermidis 12(6%) then S. hominis 7(2.8%). The results of antibiotics susceptibility showed that Staphylococcus spp isolates were susceptible to Cefuroxime and Ceftazidime (89% and 88%, respectively). Pseudomonas spp isolates were susceptible to Cloramphenicol (94%). Burkholderia cepacia isolates were highly susceptible to Ceftazidime (80%) while Kocuria sppe were susceptible to both Cefuroxime and Levofloxacin (96%). Enterobacter spp isolates were susceptible to Neomycin (81.4%) while E.coli isolates were susceptible to Levofloxacin and Neomycin (66%). Conclusion: The bacterial profile of extra and intraocular infections included the majority of conjunctivitis and vitreous fluid infections followed by corneal and aqueous humor. Gram-positive bacteria were the most prominent pathogens for external and intraocular infections.
217 Determination of hepcidin (25) level in hemodialysis Iraqi male patients and its relation with erythropoietin and some biochemical parameters, Lamia S Ashoor, AF Altaie, Qais A Ibraheem
Background: Anemia is a common finding in patients with renal failure disease and it is, primarily, due to reduced production of iron and erythropoietin. Therefore, the aim of current study was to evaluate the relationship between serum level of hepcidin 25, on the one hand, and levels of serum iron, total iron-binding capacity, serum erythropoietin and transferrin saturation% in Iraqi male patients undrgoning haemodialysis.. Methods: Serum from75 males in the end stage of hemodialysis, as group one, and 25 Iraqi healthy malecontrols, as group two, were recruited in this study. Results: Data from current study showed significant increase in hepcidin 25 and significant decrease in serum iron, total iron-binding capacity, transferrin saturation % and erythropoietin in group one as a compered with group two. In addition, current study showed non-significant positive and negative correlations between hipcidin 25 and serum iron, transferrin saturation%, total iron-binding capacity and erythropoietin. Conclusion: Patients with end-stage renal disease need to take iron supplements to correct iron-deficiency anaemia that probably results from elevated hepcidin 25 levels in those patients.
218 Study of the preventive role of cranberry extract on some physiological parameters in male rate induced by sodium fluoride, Shatha G Azez, Naseer M Hamza
Background: Traditional medicine is still widely practiced today and modern medicine benefits from many compounds. The aim of current study was to determine the biological efficacy of the cranberry plant extract on some blood parameters specific to renal function in laboratory white rats (Rattus norvegicus), orally injected with sodium fluoride. . Methods: Current study was conducted over a period of one month, from February 2019 to July 2019. The laboratory white strain inventory was studied (albino rats, n= 25) inventory of males aged between (2-4) months and weighed between (150-250g). They were divided into five groups randomly; the first group included negative control group, the second group included positive control administered an oral dose of sodium fluoride (20mg/kg body weight) for 30 respective days. Groups III and IV were considered as test groups for the protective role of cranberry extract which were administered oral doses of cranberry extract (225, 150 and 75(mg/kg body weight plus sodium fluoride for 30 respective days. After the end of the experiment, animals were anesthetized with ketamine and the blood was collected by direct heart puncture.. The studied parameters included (body weight and serum concentrations of sodium, calcium, potassium, urea and creatinine. Results: The results of present study showed significant differences in the studied parameters. A significant increase was observed in the concentration of sodium, potassium, calcium, creatinine and urea. Also, a significant decrease was observed in the body weight (P≤0.05) in rats treated with sodium fluoride when compared to the control group. On the other hand, the results of the current study showed that the treatment with cranberry extract was accompanied by some significant changes. A significant decrease was observed in body weight and concentrations of sodium, calcium, potassium and creatine (P≤0.05). When comparing the fluoride and the cranberry extract groups, the decrease led to return of these parameters closer to the state in the control group. However, the concentration of urea was increased significantly (P≤0.05) when comparing the fluoride group with the cranberry extract group. Conclusion: It was clear from the results of this study that the extract of the Cranberry plant has a biological effect in resisting the effects of toxic substances to which the organism is exposed.
219 Direct Primary Repair of Colonic Injury inAL-Ramadi Teaching Hospital, Western Iraq, Tarek M Salih
Background: Al Ramadi district, western Iraq was one of the places subjected to military operations that exposed its residents to risk of explosions and indiscriminate fire that increased the risk of penetrating colonic injuries. Objectives: To assess the pattern andmechanism of colonic injuries, other associated organ injuries, age and sex distribution, outcome and efficacy of direct primary repair. Patients and Methods: From all patients with abdominal trauma andcolonic injuries attended Al Ramadi Teaching Hospital during the period from January2014 to September 2014; those who underwent direct primary repair were enrolled in this study. Collected data include age, sex, mechanism and site of colonic injuries,other associated organs injuries, post-operative complications and mortality rate. Descriptive statistics were used to summarize the extracted information. Results: Eighty four patients were included in this study ,their age ranged(1-65) years ,mean age (30±5) years,58(69.0%)males and26(31.9%) females, out of 65 (77.4%) patients below40 years,50 (59.5%)their age ranged (21-40) years and15(18.9%)in the range of , (1-20)years,74(88.0%)patientshadpenetrating colonic injuries,46(59.7%)had shell trauma,28(33.3%)had bullet injuries ,3 (3.6%) blunt trauma. Transverse colon wasthe most common site injured 39 (46.4%), small intestine involved in 31(37%) patients and liver 27(32.1%). post-operative complication noticed in 17 (20.2%), wound infection recorded in 8(9.5%) cases; wound dehiscence 4 (4.8%). The overall mortality recorded 2(2.4%), none of these were related to colon injury. CONCLUSION: Primary repair for colonic injuries is the method of preference for management of colonic injuries as its safe, effective and associated with good results and low mortality rate.
220 Antibacterial activity of Italic leaves Aqueous Extract Against Two Pathogenic Bacteria, Aml H Salih, Rajaa H Salih, Madeha H Hussein, Ali S Hassoon
This research was achieved to detect the antibacterial efficacy of Eruca Sativa leaves aqueous extract against Two pathogenic bacteria, Two bacterial isolates Escherichia coli and staphylococcus aureus were isolated from cases of infected wound were used in this study . The anti-bacterial efficacy of Eruca Sativa Italic leaves extract was detected by measuring the inhibition zone in millimeters in 3 plates and the average was considered. The results showed Eruca Sativa extract inhibit both bacterial spp and the mean inhibitory zone for S.aureus was 14.2 mm and for E.Coli was 9.0mm.the mean inhibitory zone of Ampicillin was 20mm for S.aureus and 21.3mm for E. coli distilled water shown no antibacterial activity.
221 Detection of Mycobacterium Tuberculosis in Peritoneal Dialysis Patients , Ahmed E Salman, Jabbar S Hassan, Qudus WJ
Background: Peritoneal TB is a variant of abdominal TB, it poses a public health problem in endemic regions of the world, this type of tuberculosis usually secondary to hematogenous spread from lung and it's difficult to diagnosis because nonspecific clinical presentation with insidious onset, it is considered one of the medical challenges' doctors’ face in the diagnostic process. Aims: This study was conducted to determine the occurrence rate of M. tuberculosis in peritoneal fluids using IS6110-based polymerase chain reaction and conventional methods. Patients and Methods:Seventy-five leftoverperitoneal fluid were enrolled in this studysamplesseparated in three tubes, the first one underwent chemical analysis, the second one was centrifuged for 10 minutes at 2000 -3000 rpm , the sediment was resuspended for direct diagnosis of Mycobacterium tuberculosis using Ziehl–Neelsen staining, loopful from the sediments were inoculated in Lowenstein Jensen media, while the third tube from peritoneal fluid was stored in – 20ºC till DNA extractions. Results: Fifteen samples (20%) out of 75 peritoneal fluid samples were detected by Ziehle-Neelsen stain (ZN), three of them were culture negative on Lowenstein Jensen (LJ) media.Positive results of peritoneal fluid samples for Mycobacterium Tuberculosis on Lowenstein Jensen (LJ) media were 11 (14.6%) out of 75 after an incubation period of 14-28 days. while PCR results improved that 28(37.3%) out of 75 samples were positive for peritoneal Tuberculosis. Conclusions: This study concludes PCR targeting IS6110, may play a major role in the diagnosis of peritoneal tuberculosis this will ensure early treatment ofpatients and restricted further dissemination of disease.
222 Effect of Isolation Source on Virulence Gene Expression, Phenotypic and Antibiotic Resistance Patterns of Clinical Isolate of Pseudomonas aeruginosa, Mohamed C Sameet, Halah A Awadh, Ahmed A Suleiman
P. aeruginosa bacteria are one of the best known antibiotic resistant bacteria that cause fatal diseases in humans as a result of its high virulence. The presence of many variations in the virulence genes of multiple strains of these bacteria has led to a great variation in the gene expression of these strains. The present study aimed to study phenotypic and genetic variations in virulence factors and their relationship to antibiotic resistance and gene expression of these factors.55 isolates were collected from different pathological sources.Virulence factors were measured phenotypically and classified into medium, weak and strong isolates in production of virulence factors.Genetic and phenotypic variations were determined. The results of the study indicated that there were many mutations that had little effect on gene expression of virulence genes and the otitis media isolates were weak in the production of virulence gene and in the gene expression of biofilm, protease and pyocyanin production as well as antibiotic resistance. From this study, we can conclude that the isolation source may affect the virulence gene expression and antibiotic resistance.
223 The Role of Gut Bacterial Cytochrome-P450 of Mosquito Larvae in Degradation of Temephosas Insecticide, Mohammed M Yaseen, Azhar C Karawan, Monyer AA Alfatlawi, Ali HD Janabi
The present experimental work was generated to test the hypothesis that if there is a role of larval-related bacteria in protecting the host larvae from larvicides via bacterial-cytochrome-P450-based degradation. Here, a group of 50 larvae of Aedesalbopictus was subjected to temephos at 0.5 mg/land ketoconazole, an inhibitor of the bacterial cytochrome P450, at200mg/50 liter(TKG), 50 larvae received ketoconazole only at 200mg/50 liter (KG), and 50 larvae that played as a control group (CG) were only subjected to the larvicide. The grouping was made in a triplicate for each group.The larvae were monitored for livability every day until the end of the experiment that lasted for 2 days.The results indicated 88% and 100% mortalities in the TKG larvae in the first and the second day respectively of the experiment. However, partial deaths were seen in the CG larvae as 30 (60%) and 20 (100%) at day 1 and 2 respectively. Moreover, 10%of the larvae died in the KG when ketoconazole was used. TKG revealed significant (p
224 LOWER URINARY SYSTEM INJURIES IN ASSOCIATION WITH PREVIOUS REPEATED CAESARIAN SECTIONS: CROSS SECTIONAL STUDY, Mohammed Ridha Joodi, Abbas FM Al-Hussainy
The most frequently practiced surgical operation worldwide is operation that is cesarean and its rate is becoming higher and higher in developing countries. It is notable that the goal of operation that is cesarean is to reduce maternal and fetal mortality and morbidity; however, the operation in itself is not free of immediate and long term complications. Cesarean section, especially if repeated, is accompanied by adhesions that can result in to bowel obstruction and difficulties in future surgical operations making the possibility of complications with these adhesions more likely. The goal behind this study has been to assess the prevalence rate of complications that are urologic in company with current gynecologic or obstetric operations done for females with old operations that are cesarean. The current cross sectional study has been conducted in the urology department in AL-HILLA teaching hospitals (Al-hilla& Al-sadeq), from Octobers 2017 to Octobers 2018.Participating females in totalwere 30 females and their range of age was from 20 to 40 years with anaverage age of 30years; 26 females sufferedfrom injury to urine bladderand 4 females complained of ureteric injury. Abnormal placenta has been encountered in 73.3 %, uterine rupture was encountered in 16.7 %, injury to urine bladder was observed in 86.7 % and ijury to ureter was detected in 13.3 %. Detection of injury to bladder has beenestablishedinside operative room whereas, that of ureter was made post-operatively. The most frequent grade of bladder injury was grade 3 being identified in 46.2 % of cases. Old recurrent operations that are cesarean increase the danger of current trauma to lower tract in company with obstetric or gynecologic operations because of associated adhesion.
225 EARLY SURGICAL AND FUNCTIONAL OUTCOMES OF TOTALPROCTOCOLECTOMY AND ILEAL POUCH IN PATIENTS WITH FAMILIALADENOMATOUS POLYPOSIS, Mohammed M Khalaf, Aqeel S Mahmood, Wasem M Shaker
Familial adenomatous polyposisis the most common adenomatous polyposis illnesses. It is an inherited autosomal illness described by the early beginning of hundreds to thousands of adenomatous polyps throughout the colon. If left untreated, all patients with this syndrome usually have cancer of the colon by age35-40years.The aim of current study was toexplore how and why a disease (FAP) process is started or maintained in a given population or place. A prospective cross-sectional study carried out in Gastroenterology and Hepatology Centre at Teaching Hospital and Baghdad Teaching Hospital/Baghdad, Iraq. Data collection extended from 1st of august 2016 to 1st of February 2018. Data collected included patient's name, age, gender, phone number and address, family history, past medical and past surgical history, clinical examination. In addition, investigations performed included hematological, biochemical, radiological and endoscopic tests. Also, information gathered from the patients before surgical interference. Patients underwent 3 surgical options according to biopsy results and surgeon's preference intra-operatively. The latter included total proctocolectomy with ileum-anal pouch anastomosis, total colectomy with ileum rectal anastomosis or total proctocolectomy with continent ileostomy. A total of 11 patients (6 males and 5females) aged 18-44 years old. Family history was positive in 10 patients, and negative in one male patient. Complications occurred in an equal frequency for fistula, stricture, leak and sepsis with a 9.1% for each, while there were no complications of ischemia, paucities and obstruction. Also, 45.5% of patients developed ileal pouch-related complications, as perianal sepsis in 18.2%, wound infection in 18.2%and intra-abdominal abscess in 9.1%. Moreover, Ileostomy was done for 7 patients (54%). Restorative proctocolectomy with ileal pouch anal anastomosis is the procedure of choice for patients with familial adenomatous polyposis. Keywords: iliostomy, proctocolectomy, adenomatous polyposis, fistula
226 CLINICODEMOGRAPHIC STUDY OF PHENYLKETONURIA (PKU) IN AL‐EMAMAIN AL‐KHADIMAIYN MEDICAL CITY (RARE DISEASE UNIT) BAGHDAD, IRAQ, Mays Al-Tai, Hala Arif, Ammar Hallumy
Phenylketonuria (PKU, omim #261600) is a disorder affecting the aromatic amino acid phenylalanine. It results from a deficiency of phenylalanine hydroxylase (PAH) if un treated early in life lead to disabilities and mental retardation. The incidence of PKU is variable, being high in Turkey (around 1 in 2600 births) and low in Japan (1 in 120,000 births). The aim of thisstudy is to revise the clinical and demographic profile of the PKU patient Registered in therare disease unit in Al ‐Emamain Al‐Khadimaiyn Medical City / Baghdad. This is a single center observational study of 23 PKU patients who were registered in the (Rare disease unit), the phenylalanine level was measured as driedblood spot (DBS) either through the NBS or for symptomatic patient beyond 3 months of age send to St. Joseph university metabolic laboratory (Beirut). There was 15 males (65.2%) and 8 females (34.8%), mean age (1.3 year), Mean phenylalanine level was (16.4 mg /dl) withrange (3.5 ‐29 mg/dl), the most frequent type of PKU was moderate type 54.5%, microcephaly and mental retardation was found in totality of patient whose initial diagnoses was after six months of age, seizure was documented in nine of them. While patient who were diagnosed before six month (10 patient) we have only two patients were symptomatic although diagnosed by NBS and management started early. Twelve of the studied patients were able to undergo further evaluation including ( EEG study and or MRI of brain), all were symptomatic.PKU is a prevalent problem in Iraqi society as this sample represents a single center records Lower morbidity was observed in our studied sample, in comparison with some records from surrounding countries, owing to the early diagnosis through the screening program. Still some may proceed to have brain damage. NBS program need to be expanded to cover the remaining governorate of Iraand more enhancements of laboratory facilitiesincluding the provision of petrin test in urine and genetic analysis for the fulfillment of the diagnosis.
227 THE MOST PROFOUND TRANSCRIPTIONAL FACTOR OF BREAST CANCER AND ITS CORRELATION WITH TRADITIONAL IMMUNOHISTOCHEMICAL RECEPTOR BIOMARKERS IN IRAQI WOMEN, Reyadh S Mohammed, Israa M Al-Sudani
Breast cancer is considered the commonest type of malignancies diagnosed in females and the 2ndleadingcauseof mortality in the world. The transcriptional factor gata binding protein 3 is an important nuclear markerplays asignificant role in normal development of many human tissues involved mammary epithelial cells. In mammary glands, mutations in gene of this transcription factor leads to insufficient of its function which is finally affected in tumor suppression and leads to breast tumorgenesis. Our objective in this study revolved around the correlation of immunohistochemical expression of transcription factor with traditional immunohistochemical receptor biomarkers (hormonal receptors) and ErBb2in Iraqi women with pathological lesions of the breast. This study involved 75 casesof invasive breast lesions. Age of patients ranged from 23-75 years at the diagnosed time. These cases were graded according to Nottingham grading system. Statistical significant association reported between transcription factorexpression with nuclear hormonal receptors (p= 0.0001), while inverse association with ErBb2 (p= 0.0001). This transcription factorhas a positive association with hormonal receptors and an inverse correlation with ErBb2, lymph nodes and histology grade. Its means that this factor was associated with good prognosis elements of breast cancer.
228 OPEN LIMITED DISCECTOMY FOR LUMBAR DISC PROLAPSE; SHORT TERM CLINICAL OUTCOME, Ali Saleh Mahdi Aljanabi, Mousa I Alghazali, Muna Zamil Alshabbani
Prolapsed intervertebrallumbar discshappens in about 5-10% of patients presented with backache and contributes to considerable morbidity withsignificant economic and functional loss. It may occur at any age, but is uncommon in the very young and very old. After failure of conservative treatment trial, the mainstay treatment of lumbar disc prolapse is discectomy which can be done by different methods. The aim of study is to evaluate the short term clinical outcomesof open limited discectomy for lumber disc prolapse. This is a prospective cohort study included 42 patients who are suffering from back pain and or leg pain due to lumbar disc prolapse treated surgically by the samesurgical team at Al-Diwaniyah Teaching Hospital from October 2016 to October 2018.Clinical assessment was done for them using preoperative and postoperative Japanese Orthopaedic Association Score (JOAS). Additionally MacNab criteria were used to determine the functional improvement for all patients wereopen limited discectomy for theme was done at the time of their final follow up. All patients were satisfied with their treatment. The mean±SD for their pre-operativeJOAS was 9.02±1.38which improved to 23.19±1.19 after the operation,these improvements were statistically significant (P
229 Phylogenetic Analysis of Human Bocavirus Isolated from Children with Lower Respiratory Tract Infections in Baghdad, Iraq, Duraid A Hassan, Areej A Hussein, Qasim S Al-Mayah, Iman M Aufi
Acute respiratory infection is the major cause of morbidity and mortality worldwide, several types of viruses associated with this disease. This study aims to determine the infection rate of human bocavirus in children with lower respiratory tract infection and identify the genotyping among study population. Cross sectional study which consists of 122 children under five years old suffering from lower respiratory tract infection (75 males and 47 females); their aged from range 1 month to 60 months. They were attending respiratory wards in Fatima Al-Zahraa Hospital, Al-Elwiya Pediatrics Hospital, Ibn Al-Baladi Hospital and Pediatrics Protection Hospital in Baghdad, during the period from December 2017 to February 2018. Nasopharyngeal samples were collected from each participant, then used for DNA extraction and amplification with specific primers. Out of all 122 samples, eight samples were positive for HBoV (6.6%). Most infection were recorded in males 5(62.5%) and patients in age group 1-30 months 7(87.5%). The results of phylogenetic analysis for HBoV DNA isolated from nasopharyngeal swabs revealed all local isolates (8 isolates) are with HBoV type 1, half of the local isolates (4) were closed to Iranian isolation and the other four were closed to isolates from different regions (Tunisia, Italy and Argentina isolation). Some variant in amino acid noticed after alignment to the local isolates with some Gene Bank isolates and deletion of amino acid occur in isolates number one and five in more than two position but the substitution revealed in isolate number six in three position. In conclusion, infection rate of HBoV-1 comparable with result of neighboring countries, local isolate of HBoV-1 have their characteristic genetic structure which differentiation them from other international isolate.
230 Serum leptin level evaluation in a sample of Iraqi patients with inflammatory bowel disease, Ali S Musa Al SHAMMAA, Haithamkaddouri Hussein, Basim A Askar
Adipocytokines display a vital role in inflammation and metabolism, body metabolism changes in inflammatory bowel disease (IBD), so we must investigate the level of serum leptin in patients how had active or non-active Crohn's disease (CD) and ulcerative colitis (UC). The aim of this descriptive study is to evaluate the serum leptin level in a sample of Iraqi patients with IBD of variable disease activity index. The serum concentration of leptin was studied in 79 patients diagnosed as IBD 41 patients diagnosed as CD and in 38 persons diagnosed as UC. The diagnosis based on colonoscopy, CT, MRI, US and histopathological examination of the mucosa. Serum leptin and CRP were measured by an indirect ELISA. The leptin level was compared with CRP and correlated with disease activity and localization. Our data showed that out of 38 patients diagnosed as UC, 29 (76.3%) patients had left-sided colitis, while those with pancolitis were 9 (23.7%). On the other hand, out of 41 patients diagnosed as CD, 10 (24.4%) patients had colonic involvement, 10 (24.4%) had ideal involvement, and the remainder 21 (51.2%) had an ileocolonic extension. serum leptin in the UC group was significantly associated with disease activity in an inverse pattern (p = 0.045), while there was no significant association between leptin level and activity index in the CD group (p = 0.773). while no significant association between the action index in patients with Crohn’s disease and leptin level P-value = 0.77. No significant correlation was found between serum leptin activity and anti-TNF treatment in both UC (p = 0.262) and CD patients (p = 0.894). To conclude, there was a significant association between disease activity in UC and CD and serum leptin level (inverse relationship), but there was no association had been found with CD patients.
231 Determination of some visfatin hormone level and lipid profilein some breast cancer patients in Samarra city, Aseel Mokdad Hatam Abdulwahed1, Mahmood I. M. Alkanaani,Asmaa HassanJuma Alsamarrai,Mohammed Abdul-Monaim Hamad,AbrarHusham Dakhee, TahaYousifAhmed , Marwan Q.AL-Samarraie
Our study aimed to estimate visfatin hormone levels and lipid profile levels in some patients. The study group included 20 women with breast cancer and a control group of 10 healthy women. The hormonal and biochemical assays include visfatin hormone, total cholesterol (TC), TGS, LDL-C, HDL-C, and VLVL-C. The results showed a significant increase at (P≥0.05) in visfatin, TC, TGS, LDL-C, and VLDL-C in breast cancer patients compared to the control group, while HDL-C didn’t show any significant difference between both groups.
232 The role of brain MRI in status epilepticus, Yahya Mohammed Yaseen, Dr. Noor Kathem Nee'ma Al-Waely , Hasan Azeez Al-Hamadani
Magnetic Resonance Imaging (MRI) is increasingly being used as an investigation tool in status epilepticus (SE). It measures edema and cytotoxic edema that includes both cortical and/or subcortical constructions. The objective of our study is to identify the brain MRI changes that occur in patients with status epilepticus. A cross can aid in understanding ictal/postictal pathophysiology. Peri-ictal abnormalities may apparent as vasogenic -sectional analytic study conducted on patients with status epilepticus admitted to the emergency room or neurological ward at Al–Imamain AL-Kadhymain Medical City and AL-Yarmook Teaching Hospital for the period between January 2018 and January 2019. Brain MRI exam was performed for eligible patients using 3 Tesla MRI unit utilizing standard imaging protocol consisting of the following sequences: axial T2, coronal FLAIR, axial and/or sagittal T1, DWI and ADC maps with additional sequences tailored by the available clinical data and the imaging findings. Patients with positive MRI findings attributable to status were followed up by an MRI exam after 3 months. 28 patients were included in this study. The mean age of the patients was 40.6±18.4 years and the age range 18-80 years. Female to male ratio was 2.1:1. The frequency of MRI changes in SE patients was 3 out of 28 (10.7%). History of epilepsy was present among 28.6% of patients with SE. The primary etiology of status epilepticus for patients with MRI changes were a remote ischemic stroke for one patient and idiopathic etiology for the other two patients. Follow up MRI showed that two patients (66.7%) had reversible MRI changes, while one patient (33.35%) had mixed reversible and irreversible changes. A significant negative association was observed between the time interval from SE onset to MRI and the development of SE-related brain MRI changes (p=0.02). Our results show that 10.7 % of patients with status epilepticus had positive brain MRI changes attributable to status. The earlier an MR exam is performed for these patients, the more likely that MRI changes will be observed.
233 Serum levels of Asymmetric dimethyl arginine and Nitric oxide in patients with prediabetes and type2 diabetes mellitus, Basheer Sultan Dayir, Mohammed I Hamzah, Mahmood SH Khudair
Background: Diabetes mellitus is a global disorder, characterized by in elevation blood glucose level (hyperglycemia) for extended period of time, resulting from absolute or relative insulin deficiency. Pre-diabetes is determined on the basis of glycemic parameters which are above normal but below diabetic value.Nitric oxide (NO) is one of the major endothelium derived vaso-active substances whose role is of prime importance in maintaining endothelial homeostasis. Low levels of NO are associated with impaired endothelial function. Asymmetric dimethyl arginine (ADMA) ,an analogue of L-arginine, is a naturally occurring product of metabolism found in human circulation elevated levels of ADMA inhibit NO synthesis therefore impair endothelial functionand thus promote insulin resistance and atherosclerosis. ADMA levels are increased in people with diabetes mellitus, hypertension, chronic renal failure and dyslipidaemia. Objective: To evaluate asymmetric dimethyl arginine (ADMA) and Nitric oxide (NO) in patient with type2 diabetes and prediabetes. Subject and Methods: One hundred (100) subjects were included fifty(50) with type2Diabetes mellitus and, fifty(50) with prediabetes compared with fifty(50) healthy controls matched age and gender. The concentration of asymmetric dimethyl arginine was determined by High performance liquid chromatography method. The concentration of nitric oxide was by quantitative colorimetric according Griess method. Results: The mean concentration of asymmetric dimethyl arginine was significantly higher in the diabetic and prediabetic group compared with the control group(35.89± 2.79 µg/ml,22.98±1.86 µg/ml12.69± 1.65 µg/ml, p
234 Nitrofurantoin Side Effects Reduction by Using Royal JellyProduced by Honey Bee Workers, Majeed Hameed Nawar, Mena Waleed Hatem
Nitrofurantoin is a safe antimicrobial therapy, which is most effective in the treatment of Urinary Tract Infection (UTI) wide affecting on different types of E. coli. In this study, micewere grouped into 4 treatments, the first was used as control, whereas the2nd, 3rd and 4thweretreated with Royal Jelly RJ, Nitrofurantoin andwith a mixture of royal jelly and Nitrofurantoin, respectively.There was a significant increase in the RJ group in each of the following parameters: sperm count, sperm viability, sperm motility, Luteinizing (LH), Follicle- Stimulating (FSH) and testosterone hormones.While the abnormal form of sperm was significantly reduced, and at in the Nitrofurantoin group was the opposite, where each of sperm count, viability and movement and Malondialdehyde (MDA) decreased, but at the abnormal sperm , FSH , LH and testosterone hormone as well as the level of CAT, SOA enzyme there was a significant increase.so Finally, it can be concluded from this study that,RJ can be used to reduce the harmful role of Nitrofurantoin on sperm properties, levels of sex hormones and levels of antioxidant enzymes.
235 Intestinal parasitic stages transmitted by the American cockroach and domestic flies in the city of Tikrit, Alaa Emad Tawfeeq
The current study was conducted during the period from December 2018 to April 2019 to diagnose the intestinal parasites in the gut of the domestic flies Muscadomestica and the American cockroach Periplaneta Americana L. The samples were collected from different areas of the city of Tikrit, and the number of samples of flies collected was 92 with 110 American cockroaches and all were fully developed insects. The results of the study showed that 27 of the totaltested flies with a percentage of 29.347% were infected and 30.909% American cockroaches with a number of 34 infected insects. The parasites were identified according to the following percentages: Entamoebahistolytica (6.304%), Iodamoeba butschlii (4.347 %) and Ascaris lumbricoides with (9.782%) in domestic flies, while Entamoeba histolytica (16.363%), Giardia lambelia (4.515%) and Ascaris lumbricoides with 10% in American cockroaches.
236 De-adhesion of Common Carp Fish Eggs (Cyprinus carpio L.) Using Strawberry Natural Juice Solution During Artificial Reproduction Process, Kadhim O.M. Al-Humairi
One of the most important problems during artificial reproduction of Common carp is the adhesion of its egg. Disposal of adhesive material on eggs increasing its hatching rate activity. In this research three different concentrations of Strawberry Juice were used (10, 15 and 20% respectively) to treat the eggs of Common carp fish in order to remove the adhesive materials comparing with the control (salt, urea and Tannin solutions). The results showed that using Strawberry Juice after eggs fertilization with concentration of 20 and15 % have the possibility of removing the adhesive substances with short time of about 5 and 7 minutes respectively with significant effect ( p>0.05) as compared with the control treatment which it takes about75 minutes to remove this substance. While the concentration of10 %the adhesive materials were not removed. Additionally there was a surpassing effect (p>0.05) at the rat of eggs fertilization and hatching in the both concentrations (15%; 20 %) which was higher than that treated with Tannin and fertilizing solution, and the results also showed that eggs at the two treatments (T2 and T3) with Strawberry Juice , have a shorter period of incubation which was(66 and 64 hours)as compared with the control (71 hours to hatches at 22Cº).
237 The effect of Ramadan fasting on body weight, fasting blood sugar and lipid profile of normal male healthy non obese male medical college students in Tikrit city., Abdulrahman Jihad Mansoor
Background: Fasting during the month of Ramadan is one of the five fundamental pillars of Islamic practices, mandatory for all healthy adult Muslims. In Ramadan, Muslim adults fastfrom sunrise to sunset and are required to refrain from oral intake of food, water, beverages, smoking and sexual intercourse. This type of fasting is defined as periodic food and water deprivation during day light hours with free access during the night for the duration of one month.The aim of the study is to The effect of Ramadan on body weight, fasting blood sugar and lipid profile of normal healthy none obese male medical college students in Tikrit city. Subjects and methods: A prospective study was carried out on thirty normal healthy male students aging between 20 to 22 years, in college of medicine-Tikrit university were participate in the present study. The volunteers were allowed to consume whatever they wanted & decrease fat intake in diet.The study was conducted in the month of Ramadan from beginning of May to 5th of June /2019. Average duration of the fast was about 13.5 hours & maximum temperature ranged from 25 to 35 C.Body weight was measured to the nearest 100gm. Fasting blood sugar and lipid profile were measured at beginning and the end of Ramadan and one hour before break fasting (one hour before sunset). Results: There were slight decrease in body weight and body mass index (BMI) at the end of Ramadan fasting as compare with beginning of Ramadan fasting. Also, in regard to blood parameters, there is no significant difference regarding blood glucose from at end of Ramadan as compare with the beginning of fasting. Also, there were significant reduction in serum TG, total cholesterol, LDL and VLDL at the end of Ramadan fasting as compare with beginning of Ramadan fasting. However, There was significant elevation in serm HDL at the end of Ramadan fasting as compare with beginning of Ramadan fasting.
238 Isolation and diagnosis of parasitic stages isolated from house dust in some areas of Salah al - Din governorate, Iraq, Ashraf Jamal Mahmoud Zangana
The total number of samples taken from 473 samples of the dust of randomly assigned residential houses in some areas of Salah al-Din Governorate (Tikrit, Samarra, Durr, Al-Alam) for the period from October 2018 to the end of March 2019 to detect the pollution rate of house dust by parasitic stages. And diagnosed eegs of some intestinal parasites (Ascaris lumbricoides, Trichuris trichura, Toxocara canis, T. cati) , T. canis which had the highest pollution rate of parasitic stages of with (32.317%), followed by Trichuris trichura (20.731%).The results showed that the number of positive samples was 164 with 43.850%, The highest pollution rate was in Al-Alam district where it reached 53.33%, The lowest percentage was in Samarra district,With 40%, while Tikrit districtand Al-Durr were 51.11% and 46.66% Respectively.The results showed that the highest percentage of contamination of house dust from houses in Samarra district was during February by 57.142%, the lowest was 17.647% in March, and no pollution during of December, and inAl-Alam district was the highest pollution rate of samples 60% in March and the lowest was 33.33%. and in Al-Dor district the highest percentage of contamination was during November 64.285%, and lowest percentage during December was 20% , and no pollution during March , And highest percentage of contamination in samples of Tikrit district were 57.897% during October and the lowest percentage during March was 30.769%.
239 The total number of samples taken from 473 samples of the dust of randomly assigned residential houses in some areas of Salah al-Din Governorate (Tikrit, Samarra, Durr, Al-Alam) for the period from October 2018 to the end of March 2019 to detect the pollution rate of house dust by parasitic stages. And diagnosed eegs of some intestinal parasites (Ascaris lumbricoides, Trichuris trichura, Toxocara canis, T. cati) , T. canis which had the highest pollution rate of parasitic stages of with (32.317%), followed by Trichuris trichura (20.731%).The results showed that the number of positive samples was 164 with 43.850%, The highest pollution rate was in Al-Alam district where it reached 53.33%, The lowest percentage was in Samarra district,With 40%, while Tikrit districtand Al-Durr were 51.11% and 46.66% Respectively.The results showed that the highest percentage of contamination of house dust from houses in Samarra district was during February by 57.142%, the lowest was 17.647% in March, and no pollution during of December, and inAl-Alam district was the highest pollution rate of samples 60% in March and the lowest was 33.33%. and in Al-Dor district the highest percentage of contamination was during November 64.285%, and lowest percentage during December was 20% , and no pollution during March , And highest percentage of contamination in samples of Tikrit district were 57.897% during October and the lowest percentage during March was 30.769%. , Abdulrahman Jihad Mansoor, Yaseen K Hussein, Abdulhadi M Jumaa, Mossa M Marbut
Background: Ramadan is the holiest month in the Islamic calendar. Fasting in this month is one of the five pillars of Islam. Fasting is obligatory for all adults and healthy Muslims during the day hours for the whole month every year. It is the month during which Muslims refrain from food, and liquids during daylight hours and eat a main meal after sunset. The aim of study is to investigate the effects of fasting on lipid profile, IL-6 and TNF among healthy female medical students in Tikrit University. Subjects and methods: A follow up study was done in college of medicine – Tikrit university during Ramadan fasting month at 1441, (May 2019) on female medical female student of 2nd year. A total of 20 female students in this study were female aged 19-20 years, none obese, do not have a history of diabetes, do not have hypertension and not an athlete. Female was used as subjects because they can do full time of Ramadan fasting. Female students not complete consecutive 21 days of fasting were excluded from the study. At a day one (beginning of Ramadan) and at day 21 (21th day) of Ramadan fasting, blood sampling were taken to be analyzed of lipid profile, and inflammatory indicators (TNF-α and IL-6). According standard procedures. Results: Regarding body weight, and BMI, in the present study, there is significant reduction in body weight and BMI in day 21 of Ramadan fasting as compare with beginning of fasting. In the present study, there is a significant reduction in the concentrations of serum triglycerides, total cholesterol, LDL-c and VLDL-c at 21th day of Ramadan fasting as compare to the beginning of Ramadan fasting. However, there is significant increase in the concentration of serum HDL-c at 21 day of Ramadan fasting as compare with the beginning of fasting, (p≤ 0.01). In the present study, it were found that there is significant reduction in the concentration of IL-6 and TNF- α in female subjects at day 21 of Ramadan fasting as compare with that at the beginning of fasting, (p≤ 0.01). The present study conclude that were significant reduction in the concentration of IL-6 and TNF- α in female subjects at day 21 of Ramadan fasting as compare with that at the beginning of fasting, (p≤ 0.01).
240 Surgical Repair of Atresia Ani with Congenital Recto-Vaginal Fistula in Female Calf: Case Report, Ashur KH, Majid A Alkhilani, Ali A Ajeel
A five female calves about (2-5) months old were presented with congenital atresia ani and recto vaginal fistula; these were clinically examined and recognized as a congenital anomalies. Treatment was effective. Under caudal epidural anesthesia was given with 2% Lidocaine HCL, the anal orifice was reconstructed and the fistulous tract was closed successfully. The rectovaginal fistula was repaired by placing continuous sutures in roof of vagina. Penicillin Streptomycin was injected postoperatively at a dose 10000 IU and 10mg/kg B.W intramuscularly respectively, and the anal stitches were removed after 14 days.
241 Assessment of immune response in patients with type 2 Diabetes mellitus, Raed F Al-Jobouri
Background: Humoral and complement system are important parameters of immune system and any interference with these might lead into susceptibility of infections. The study aim to measure the mean levels of serum immunoglobulins and complement components in DM (2) patients. Patients and Methods: Forty two patients who have D.M.(2) whose HbA1c was more than10 % with history of recurrent infection (more than two infections /year ) in addition to 30 apparently healthy control were included in this study. Five ml blood was drained and putted in plastic tubes in (20-25°C)(room temperature) to clot, then 5 minutes centrifugation was done, then storage was done at (-20) °C until examination by radial immunodiffusion assay until by single radial immunodiffusion assay examination(21)for the measurement of immunoglobulin (IgG, IgM, IgA) with complement constituents (C3 and C4). Results: DM(2)patients had reduced IgA,IgG,IgM,C3 and C4(122.58±14.22; 802.23±124.11; 89.74±15.22;118.39±23.5 and 39.66±12.5 respectively) in comparison to healthy control(188.8±18.34;1224.66±201.99;141.66±27.99;161.44±28.77 and53.44±14.4 respectively)(P value < 0.05). DM (2) patients with BMI >28 have C3 (134.49±23.8) in comparison to (116.6±20.3) in DM(2) patients with BMI
242 Combined effects of melatonin receptor gene polymorphism and vitamin E supplementation on litter size and lambs body weight in Awassi ewes, Atheer S Mahdi, Muneer W Al-Khafaji, Anmar A Al-Wazeer, Hamza M Al-Khuzai
The study was conducted at the period of 22/1/2018 to 13/1/2019 by using 82 Awassi ewes in late gestation period to determine the combined effect of melatonin receptor gene polymorphism (MTNRA1) three doses of vitamin E on litter size and lambs weight at birth and weaning. Results showed a significant effect (P
243 KCNMA1gene expression a promising biomarker in childhood acute lymphoblastic leukaemia, Zaid Khalid, Fadhel M Lafta, Abdul-Ameer N Ghaloub Al-Rekabi
Amplification and over expression of ions channels have been suggested to involve in tumourgenesis. This study was set to assess the expression level of KCNMA1gene, encodes for calcium-activated potassium channel, in a set of childhood acute lymphoblastic leukaemia (ALL) patients in Iraq-Baghdad. Relative expression utilising RT-PCR technique was used to estimate the KCNMA1gene expression in 31 cases diagnosed with childhood ALL. The results showed that the vast majority (77.42) of the investigated ALL cases have overexpression of KCNMA1.While only 22.58 of the cases express normal to low levels of KCNMA1 gene.Over all, the present study results suggest an oncogenic role for KCNMA1gene expression in childhood ALL and this would be assessed further in large scale studies to investigate its prognostic and therapeutic potential utility.
244 In vitro determining the effect of vaginal lactobacillus on some pathogenic of urogenital infections in women , Bushra Jasim Mohammed, Amel Abdul Aaima Kadhim
It has been postulated that lactobacillus play a critical role in maintaining the normal vaginal ecosystem by preventing overgrowth the of pathogens and other opportunistic organisms by producing lactic acid hydrogen peroxide, bacteriocines and other antimicrobial substances. Current study aimed to determine the effect of vaginal lactobacillus spp (L.acidophilus, L. crispatus, L. gasseri and L. iners), as probiotics isolated from vaginal swabs of fifty healthy women for reduction of urogenital infections(Candida albicans, Gardenella viginals, Staphylococcus aureus and Escherichia coli) isolated from female patients who attended the obstetric and gynecology clinics in Ibn-AL Baladi Hospital. The effects of lactobacillus strains supernatants agents urogenital pathogens were tested in agar plate diffusion method on Manns-Regoz and Sharpe (MRS) agar and in liquid medium (MRS broth).Also Minimum inhibitory concentration (MIC), Minimum bacteriocidal concentration(MBC) and Minimum fungicidal concentration(MFC) were determined. The result of antimicrobial activity of vaginal lactobacillus revealed that L.crispatus had the highest effect on urogenital pathogens in both methods while the result of MIC , MBC and MFC showed that the concentration 60% led to minimized growth of G. viginals, S. aureus and E. coli and concentration 70% inhibit growth of these bacteria completely , whereas sharp decrease in candida albicans growth noticed in80% concentration even as the concentration 90% and above inhibited all pathogenic isolates (MBC and MFC).From above results it be clear that all Lactobacillus strains have antimicrobial activity against urogenital pathogens but L.crispatus had the highest affect than others with promising inhibitory spectrum
245 Evaluation of serum IL-23 in multiple sclerosis in Iraqi patients, Zahraa F Shaker, Samar Abdul Raheem Al-Gharrawi
Background/aim:A chronic neuroinflammation of disease with unknown cause and variable clinical development is multiple sclerosis (MS).Interleukin-23 (IL-23) is a constituent of the IL-12 cytokine family that has shown through development of T helper type 17 (Th17) cells development could play a chief role in the inflammatory autoimmune responses in multiple sclerosis (MS).Objective:A goal of studyis to assess IL-23 level in serum in Iraqi patients with multiple sclerosis (MS) in comparison with healthycontrol group.Methods:blood sampleswere taken from 45patients with multiple sclerosis and 30 healthy controls. IL-23 level in the serum wasassessed by Enzyme-Linked immunosorbent assay (ELISA).Results:: IL-23 level in the serum taken from affected persons was found to be significantly hiegher than its level in the serum of control group (P
246 Evidence of ACTH and Prednisolone in Treatment of Bell’s Palsy, Musaed hekmat Al-Dahhan
Background: Facial paralysis is an acute idiopathic unilateral paralysis of facial nerve without associated disorders; purpose of this study was to evaluate the grading, incidence and proper management. Methods: sixty four patients diagnosed with Bell’s palsy between February 2015 to May 2017, analyses according to gender, age, etiology, functional statues as measured by a House- Backmann scale. All patients treated by limited dose of prednisolone together with tetracosactide acetate in twice dose and followed up for 6 months. Result :A total 64 patients presented with facial paralysis average of the age 35 years , 34 ( 53.1 % ) were male and 30 ( 46.8 % ) patient were female , 58 ( 90.6 % ) of patients diagnosed within 3 days of onset of symptom . All these patients treated with 0.75 mg /Kg/day with tapering along two weeks together with tetracosactide acetate 1 mg /ml weekly in twice dose and Galvanic stimulation in first week of onset of symptoms. 58 (90.6 %) patients fully recovered within 3 months of treatment while 5(7.8 %) patients completely recovered within 6 months while only one patient not recovered. Conclusion: Patents with new onset of facial nerve paralysis give higher recovery rate by using limited dose of prednisolone and tetracosactide acetate within 3 days of onset of symptoms together with physiotherapy program
247 Immunological Role of Annona muricata (Dietary Supplement of gravula) Against Cypermethrin's Toxic Effects in Female Rats, Ali N Umayra, Mohammed J Qasim, Abdul Kareem S Al-Yasari, Mohammed H Dakheel
The current research was conducted to investigate the toxic effects of Cypermethrin (CYP) on female rats and the immunological response with or without the oral administration of Graviola (Dietary Supplement) by the use gavager. Sixteen adults female rats aged 60-65 days divided equally into four groups; the first group was orally gavagede with 1 ml of sterile normal saline as a control group, the second group was orally gavagede with 14.5mg/kg B.W. of cypermethrin, while the third group was orally gavaged with 2.5mg/ml of graviola.eWhereas the fourth group was gavaged with 14.5mg/kg of B.W. of cypermethrine plus 2.5mg/ml of graviola. On day 30 the cellulare and humorale immune responses were examinede for all groupse, blood samples were gathered for phagocytic activity measurement and serum was separated by ELISA method for measuring IgG titer.The mean value of phagocytic indices in First, Second, Third and Fourth groups as follow: the first group was (22±3.83) as a control group, the second group was (13.6±2.54) that treated with cypermethrine, the third group was (89±1.82) that treated with graviolae and the fourth group that treated with cypermethrin+graviolae was (68.6±2.21); The current studye showed that the graviolaehad a marked increase in the cellular immune response through the high increase of phagocytic indices due to Toll-like receptor 4 (TLR4) activation. The result of this study showed that the mean value of phagocytic indices in rats that gavaged with CYP, decreased significantly (p
248 In vitro efficacy assessment of cauliflower (BrassicaoleraceaVar Botrytis) alcoholic extract in mortality percentage of motile stage of mite (Teranychusurticae Koch), Nidhal Abdulhadi Jaafar
The study was conducted to test the effect of cauliflower alcoholic extract in the motile stage for the two-spottedspider mite pest. The test was carried on using two methods: leaf disk spray and leaf disk dip. The concentrationsthat used in the test were(2.5%, 2%, 1.5%, 1%, 0.5%, 0%) on different stages (larvae, nymphs, adults: female and male), while themortalitypercentfor different stageswas calculated after 24, 48 and 72 hours of treatment.The study showed that there was a significant effect of the different concentrations of cauliflower extract in the different stages of the mitepest, as the (2.5%)treatment was superior over the rest of treatments by giving the highest mortalitypercentfor the different stages, for both methods, leaf disk spray, and leaf disk dip. The mortalitypercentageafter 72 hours of treatment were as follows; the mortalitypercentof larvae (100.0and92.31%), and for nymphs were (91.31 and 82.50%), while it was (72.50 and 72.00%) for adult females, and (79.49 and 77.49%) for males, respectively.
249 Toxoplasmosis in Cats: Serological and Molecular Study in Baghdad Province, Ahmed NA Al-Ani, Taha YG Al-badrawi, Zaid S Hussein
The only hosts that can excrete resistant oocysts to environment are cats which play important role in the epidemiology of the Toxoplasma gondii. About one-third of the global population infected with T. gondii which is a major zoonotic agent. A total 141blood samples collected from cats in Baghdad province, Iraq. Serological test on all serum samples were applied using latex agglutination test (LAT) were conducted to detect anti-T. gondii antibodies which showed 62.42% positive results.Age and sex regarded in this study were showed statistically no significant differences between both sexes or between different age groups.Although, the cats 6-10 years old showed highest positive results 74%.Nested polymerase chain reaction (nPCR) was also conducted on allserum samples targeting B1 gene showed 95.3% T. gondii infection.
250 Effect of addition of melatonin to the intravaginal sponges on oxidative status and reproductive performance in Iraqi awassi ewes, Ammar Rahem Mansoor, Imad Dawood Saleh, Hmeed Abed Dakheel
This study was achieved to know the effect of addition of melatonin to intravaginal sponges impregnated with progesterone on oxidative status and reproductive performance in Iraqi awassi ewes. Twelve non-pregnant ewes were selected with an age ranged between 2-4 years, and weight with a mean of 42.8 ± 5.3 kg. The animals were divided in to two equal groups. Each group contained 6 animals. The first groups were treated with sponges free from melatonin; the second group treated with sponges contained two grain of melatonin in a dose of 6mg (3mg/grain) by putting them inside the sponges through small opening. All ewes were injected with eCG (250 i.u/ i.m) after the removal of sponges on day 12. Two Rams were introduced on the next day after removal of sponges. Blood samples were collected on day 0, 3, 7 and 12 from insertion of vaginal sponges for measurements the total leukocytes count and the level of malondialdehyde in plasma during the days of treatments with vaginal sponges. The result of study showed a significant increase in the level of malondialdehyde during the days of treatments for both groups. The results also showed that there was no significant difference between two treated groups in percentages of appearance of estrus, the period from removal of sponges to the appearance of estrus, pregnancy rate and fertility rate. It was concluded from this study that the use of vaginal sponges for estrus synchronization in ewes leads to increase the level of oxidative stress and the addition of melatonin with a dose 6mg to the sponges have no effect to decrease the level of oxidative stress that leads to vaginal inflammation concerned the intravaginal sponges
251 Toxicity of Liver and Kidney Induced by Different Concentrations of Tramadol in Young and Adult Mice, Rafal N Abbas, Jamela Jouda, Assal G Alshammary, Mahdi S Jumaa
It is thought that Tramadol TM, a synthetic opioid analgesic agent, is low in abuse and has no side effects if its total daily dose doesn’t exceed 400mg. This work aims to study the pathohistological and physiological toxicity effect of tramadol in different concentrations, equivalent to less than 400 mg, on liver and kidney. Forty-eight male Balb/c mice were used in this study and divided into two age groups (n=24 mice); the first was an adult aged 8-9 weeks and the other was young aged 4-5 weeks. Every age group was divided into three subgroups (n=8 mice). The first group consumed a high concentration of TM, 40 mg TM/ kg body weight daily which equivalent about 421.2 mg TM/ 65kg body weight. The second group was consumed a low concentration of TM, 20 mg/kg daily. The last group consumed only water ad libtum and served as a control group. After one month, the mice were sacrificed. Blood samples were collected to separate the serum and used to determine; kidney function tests (S.Cr and B.U) and liver function tests (GOT, GPT, and ALP). Kidney and liver were collected and put in 10% formalin for pathohistological study. GPT, ALP, B.Ur and S.Cr levels were significantly higher in the young and adult mice consumed TM compared to control while there was no different in GOT level. Comparable results were found in the pathohistological study of kidney and liver section. The infiltration of inflammatory cells and its aggregation were detected within the kidney and liver tissue of adult and young mice group that consumed TM. These changes were severing in the mice consumed TM high concentration compared to lower concentration. As a conclusion, Tramadol could have dangerous side effects even when its total dose doesn’t exceed 400mg daily. These side effects didn’t interact with age.
252 Artemisia L. Fruit Extract Normalized Diabetic and Liver Function Parameters in Streptozotocin-Induced Diabetic Mice , Emad Mahmoud Eltayef, Jamela Jouda, Mohammed K Saeed, Ashwaq Abdul Jabbar Khadem
Many other researchers studied the effect of many part from Artemisia but not fruit on the diabetes. Artemisia fruits were used in this study, they had been dried and grinded then some chemical analyses were done to determine the active compounds of the fruits in both hot and cold extract. Since our results shows that the hot extract has more active compounds from the cold, the hot extract of Artemisia L. fruit were used in this study to determine the efficacy of it as diabetic treatment in streptozotocin-induced diabetic mice. Streptozocin (STZ) was used to induce diabetes in 35 mice which divided to 5 groups. One group consumed normal saline while the other four consumed Artemisia with four different concentrations. Another 10 mice weren’t injected with STZ which were they had been divided to two groups; one consumed Artemisia and the other consumed normal saline. The fasting blood sugar (FBS) was determined on different period; zero time, 7 and 14 days after treatment. The mice were scarified and blood was collected. Insulin, GOT and GPT levels were determine in serum. FBS was higher in all STZ-induced diabetic mice dropped to normal after 14 days. The FBS of mice, treated with 500 Artemisia/ kg mice, was closer to control. Insulin level was lower in STZ-induce diabetic mice without treatment raised to normal after treatment with 500 and 1000 mg Artemisia/ kg mice. Same results were found for GOT and GPT levels. As conclusion, Artemisia appears to be a good modulator for diabetes especially at 500 and 1000 mg Artemisia/ kg body weight.
253 Kinetics of Lipoprotein-associated phospholipase A2 (Lp-PLA2) enzyme And copeptin peptide level as a new biochemical marker For acute myocardial infarction patients , Raghad A Mohammed, Rayah S Baban, Moayed Basheer
Background: MI is defined as myocardial cell death caused by prolonged ischemia. Delay in diagnosis and management results in high mortality, and prompt early diagnosis rates and interventions may be life-saving. Early identification of myocardial infarction in patients with atherosclerotic cardiovascular disease (ASCVD) has great clinical significance. Objective: To evaluate kinetics of Lp-PLA2 (Km and Vmax) enzyme, copeptin and cTnI levels in acute myocardial infarction patients and control group which may become a useful biomarker for predicting acute myocardial infarction (AMI). Subjects and methods: A case-control study was conducted at the Department of Chemistry and Biochemistry, College of Medicine, Al-Nahrain University and cardiology department Al-Amameen Al-Kadhimin medical city, Baghdad, Iraq, between December 2018 and April 2019. The institutional review board (IRB) of the college of medicine, Al-Nahrain University, Baghdad, Iraq approved this study. Written informed consent was obtained from all patients for participation in the study. The kinetic study of LP-PLA2 and the levels of CPP, cTnI, CK-MB, and CRP were measured for two study groups: 40 patients with myocardial infarction within 48 hours from symptoms before treatment, compared with 40 healthy participants as a control group. Only five milliliters of blood were collected from the participants. The LP-PLA2 assay was measured by Glomax-Discover/Promega USA using LP-PLA2 Kit (colorimetric).The CPP, cTnI, CK-MB, and CRP were measured using Glomax-Discover/Promega USA, ELISA technique. Results: The kinetic of the LP-PLA2 in AMI group were elevated in AMI group than control group, (Km=4.98 µM, Vmax=12.9 mmol/L/sec vs Km=4.21µM, Vmax=8.12 mmol/L/sec). CPP, cTnI, CK-MB, LDL were found elevated in patients with AMI compared with control (p>0.001). Which Copeptin was more specific marker, specificity= 83.0%). Conclusions: 1- This study shows there is a significant elevation in the enzyme kinatic of LP-PLA2 in the MI group compared with healthy control. 2- Combined measurements of troponin and copeptin aid in the early diagnosis and safe rule-out of acute myocardial infarction.
254 Detection of OMPA gene of Enterobacter sakazakii from dried milk sample in Iraq, Mohammed Mosa Jaafar, Salwa K Kalid, Mustafa Hassan Z Khetam, Habeeb Rasooll
This study aimed isolation and Identification of Enterobacter sakazakii from powder milk for children under 2 years. Total of 30 triplicates samples, were collected were taken from local pharmacies different locations in Baghdad during the period between 1\2\2018 to 1\9\2019. The study obtained 19 isolates confirmed to E. sakazakii (17.1%) from milk (4 isolate from Dialak1, 1 Novolac Allernova and 2 Novolac AD, 3 Celia 1, 2, 1 Nursoy, 2 Nactalia, 3 Novolac AD, 3 Novolac 1. The revauted that all isolates had ompA Gene (100%).
255 Value of high resolution CT scan of the lungs in the assessment of severity of COPD, Shameem Ali Hussein
Background: Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease affecting the airways, leading to significant morbidity and mortality throughout the world. Aim of study: To evaluate the severity of the COPD by using high resolution computed tomography (HRCT). Patients and methods: a cross sectional analytic study conducted in CT unit of Radiology Department at Imamein Alkadhmeim Medical City through the period from 1st of September, 2017 to 1st August 2018 on sample of 40 patients with COPD. After taking full history and examination, HRCT scan for chest was done. Results: The CT scan scoring revealed that mean total score was 27.3±12.7; 40% of patients (16 patients) had mild COPD, 47.5% of patients (19 patients) had moderate COPD and 12.5% of patients (16 patients) had severe COPD. There was a highly significant association between increased age of patients and severe COPD (p=0.001). No significant differences were observed between patients with different severity of COPD regarding gender of patients. There was a significant association between longer smoking duration and severe COPD (p=0.006). There was a significant association between increased daily smoking amount and severe COPD (p=0.006). A highly significant association was observed between longer COPD duration and severe COPD (p
256 Determination of Cathelicidin in UTI patients of Basrah province, Amna Jawad ALhamedy, Wafaa Sadoon Shani
Cathelicidin is important components of the innate defense in the urinary tract. The aim of this study was to characterize whether these anti -microbial peptide are important for developing urinary tract infections (UTIs). This aim was investigated by comparing blood urinary peptide levels of UTI patients’ infection to those of controls. A case- control study was conducted at Basrah province (Basrah general hospital and Al-Sadr Educational hospital) during the period from 18 November 2018 to 15 April 2019. 60 patients with confirmed UTI and 30 healthy controls without UTI. Plasma and urine levels of cathelicidin were determined using an enzyme linked immunesorbent assay (ELISA) kit. The mean concentration of anti-microbial peptide cathelicidin (AMPccl37ng/ml) was highly significant P≤0.001, P≤0.000 in urine and sera respectively, with no significant difference correlation between the type of bacterial infection and concentration of ccl37 in urine and sera. Conclusion Urinary cathelicidin is microbial markers that may assist the diagnosis of UTI in woman.
257 Cytopathic effects of activated parasporal inclusion proteins produced from Iraqi isolates of Bacillus thuringiensis, Marwa AK Hadab, Bareq N Al-Nuaimi, Ammar Basim Al-Asadi, Jabbar F Al-maadhidi, Murtadha Nabeel Abdul-Gani
Six isolates of Bacillus thuringiensis were isolated from Iraqi soil characterized as non- insecticidal and non- hemolytic parasporal inclusion proteins. Bacterial isolates were propagated on nutrient broth. Then, the parasporal inclusion proteins were extracted and processed with proteinase K and trypsin. The major protein segments produced of 64KDa were characterized and tested for cytocidal activity against human leukemic T- cells (CLL) (Chronic lymphoid leukemia). Results indicated that the treated parasporal proteins of four isolates (Bt2, Bt3, Bt4 and Bt6) showed strong cytotoxicity with no significant differences between normal lymphocytes and leukemic lymphocytes. Two isolates BtA1 and BtA5 show discriminative cytotoxicity between normal and leukemic lymphocytes at low extant causing cell ballooning, nucleus fragmentation and finally cell rapture. This may be referring to the ratio of unsaturated fatty acids included in cell membrane.
258 Effect of Cinnamon and Turmeric Nanoparticles Extract in Quality Characteristics of fresh Ground Beef during cold Storage, Ahmed Sami Mohammed Al-Salmany, Amera Mohammed saleh Al-Rubeii
The objective of this study was to evaluate the effect of adding different concentrations of cinnamon silver nanoparticles (CAgNPs) and turmeric silver nanoparticles (TAgNPs), on oxidative stability and quality characteristics of fresh ground beef, stored at 2°C for 12 days. In current study, six different treatments of (CAgNPs) and (TAgNPs) were analyzed. These treatments include, T1 positive control treatment (add water), T2 negative control treatment (without addition), T3 (turmeric 248.3 ppm (, T4 (turmeric 496.9 ppm), T5 (cinnamon 83.08 ppm (,and T6 (cinnamon 166.16 ppm). The treatments were stored for 1, 4, 8 and 12 days. Results were analyzed after the physical and chemical tests conducted. The results of present study were noted that: the addition of Cinnamon and turmeric nanoparticles extracts to ground beef, during cold storage period led to a significant increases (P
259 Effects of ZnO NPS on Streptococcus pyogenes in vivo , Luma S mohammed, Mais E Ahmed
In vivo study revealed that ZnO nanoparticles treatment of Streptococcus SPP contaminated injured skin showed good prognosis and good healing process include complete regeneration of the epithelial cells of the epidermis and increase of cellulartiy of the dermal content compared with untreated group. In conclusion, treatment of S. pyogenes infected skin with Zinc oxide nanoparticles concentration (2 mg/ml) limit the skin damage and localized the lesion to the incision site with good healing process.
260 The Importance of Competitive Priorities on Rationalizing Product Costs and Achieving Customer Satisfaction in the Industrial Sector, Mohammed Sameer Dherib Al Robaaiy
The research aims at explaining the reasons for the high cost of production and the application of modern concepts in accounting costs and administrative, which came to remove loss and waste and rationalization of costs and improve operational performance and in response to changing conditions in the business environment. As a result of these variables and circumstances, the research problem was manifested in the high cost of manufacturing the products of Ur Company, which lost competitive precedence with other products in terms of price, quality and other specifications imported from various sources. In order to achieve the objective of the research and solve the problem was developed a basic hypothesis that the use and application of modern concepts lead to rationalize the cost of manufacturing products and improve activities and processes and create a market share in the local environment. Divided of research into three topics the first topic reviewed the theoretical aspect of modern concepts and competitive priorities, including rationalization of costs and removal of loss and improve performance and address the negatives in traditional concepts and methods. Either the second topic dealt with how to rationalize the cost of some of the products of the electrification plant, either the third section, he concluded with a number of results, the most important of which the high proportion of production costs, especially the proportion of direct wage costs to the total cost of manufactured goods (37%)Very high percentage and low competitive precedence prices of local products of the company compared to the prices of imported products, and the obsolete equipment and machines in the factory. This caused a significant increase in the cost of maintenance and backup tools. A significant reduction in actual production levels from the planned annual level of energy. It is also necessary to continuously seek to reduce the production, marketing and administrative costs of the plant, the need to seek replacement of machinery and equipment with advanced technology machines, and to impose customs duties on the imported product and activate the role of the standardization and quality control apparatus.
261 Studying the inhibition effect of some food additives against pathogenic bacteria, Muhsin Hamad Edham, Nehan B Jafar, Zainab H Fadhil
Latterly, natural products have been used as antibiotics and have proven effective against a large number of microorganisms. The present study included the measurement of antibacterial activities of pomegranate juice or molasses, tamarind molasses, garlic oil with thyme and with chili pepper on the five selected bacteria (2 Gr+ and 3 Gr-) by disk and agar well diffusion assays on the Muller Hinton Agar (MHA) and Blood Agar (BA). Pomegranate and tamarind molasses exhibited a broad spectrum of anti-bacterial activity inhibiting both the groups of bacteria. Pomegranate and tamarind molasses has shown highest antimicrobial activity compared to garlic oil with herbs. Inter- selected of bacterial cultures, the highest antibacterial effect of pomegranate and tamarind molasses by using disk diffusion was recorded against E. coli, Staphylococcus aureus, Streptococcus mutans and Pseudomonas aeruginosa (20,18,20,12 mm) (20,20,20,17 mm) respectively while, when used agar well diffusion assay to tested antibacterial activities of pomegranate and tamarind molasses the result show rather increased inhibition zone of selected bacteria but, bacterial culture of Klepsiella pneumonia recorded resistance against food additives which can used in this study.
262 The effect of tactical exercises in different playing centers on some of the motor abilities of young football players, ages (17-19) years, Muhammed Mussa Lazim Al-Essawi
In recent years, the football game has witnessed a great development in different playing styles, and this has become evident in the recent football tournaments. Tactics and plans have played a big role in the results of matches. Modern play has become more difficult and complicated. Then the preparation of the team became difficult, and the training became more complex and difficult. This in turn forced the football training scholars to search for new methods and methods of training, so the training diversified and became close, or even similar, to the actual competition during the match, which led the coaches to resort to collective and planning exercises to develop the physical, motor, and planning aspects, and the research problem was that most of the vocabulary Training curricula for youth soccer teams It became clear not to allocate spatial time in the special preparation stage for such planning exercises, but even that most coaches follow the old methods only in the player acquiring physical fitness and skills in one direction or a specific capacity or ability and this is what we call Now the modern ball (exercise non-economic). The aim of the research was to prepare tactical exercises in the various play centers for youth soccer players of ages (17-19) years. The research hypotheses were statistically significant differences between the results of the pre and posttests of the research sample (control and experimental) in some of the motor capabilities of youth soccer players. Theoretical studies were related to tactical exercises, defensive tactical exercises, offensive tactical exercises and kinetic abilities. The research sample was (16) players from the border club youth ages (19-19 years) and the search tests were the kinetic ability test (accuracy) and the kinetic ability test (agility). The researcher discussed the results that appeared to him in Chapter Four and from the research findings, the tactical exercises in the various playing centers achieved a significant development in the motor abilities (accuracy and agility) of the experimental group when comparing the results of the pre and posttests and from the recommendations of the research is the adoption of completeness plans in the various play centers prepared by the researcher to train football players to develop motor abilities.
263 Determine the ideal conditions for the biodegradation process and lowest inhibit concentration of Clorspan and Ground up pesticides by different types of Bacteria, Beadaa Abdalqader Mahdii, Ahmed Mohammed Turke
Local bacterial isolates isolated from 23 soil samples polluted with different pesticides from Baghdad areas on both sides of Karkh and Rusafa. The ability of bacterial isolates on biodegradation of (Clorspan (Chlorpyrifos) and Ground Up( glyphosate)) pesticides examined , where a preliminary examination of bacterial isolates was performed by using solid and liquid MSM for the purpose of determine the better Bacterial isolate which able to biodegradation of pesticides with a concentration of 100 mg / liter of pesticide with a pH 7 and 37 ° C temperature , where the Pseudomonas aeruginosa bacteria proved it efficiency as a bacterial isolate able to analyze Clorspan and Ground up , And for (MIC) experiment the lowest inhibiting concentration of the bacteria growth determined. Since the results indicated that Pseudomonas aeruginosa bacteria continued to grow for all concentrations and both pesticides that start from (50-500) mg / liter. As for Staphylococcus bacteria, the lowest inhibitor concentration was 450 mg/ liter in Clorspan, and for 500 mg / liter for Ground up. As for the ideal conditions, Pseudomonas aeruginosa proved to be the best isolate bacterium which able to analyze pesticides biologically by registering highest values , it recorded value of (95,53,50, 27) in ideal temperature , in 45 ° C for the Clorspan and Ground up pesticides, respectively, and (43.30) During cuddling period for 7 days in clorspan pesticide medium and (39.15) During cuddling period for 8 days in Ground up medium and it recorded (27.87) for pH7 in Clorspan medium. And for Ground up pesticide medium, it recorded a highest values for Staphylococcus bacteria and it was (34.25) at pH7. The study aimed to determine the best bacterial isolate which able of biodegradation of pesticides, and determine MIC (Minimum Inhibition Concentration) for bacterial growth in different pesticide concentrations , Study the ideal conditions for biodegradation of pesticides (temperature, PH, Cuddling period).
264 Local bacterial isolates isolated from 23 soil samples polluted with different pesticides from Baghdad areas on both sides of Karkh and Rusafa. The ability of bacterial isolates on biodegradation of (Clorspan (Chlorpyrifos) and Ground Up( glyphosate)) pesticides examined , where a preliminary examination of bacterial isolates was performed by using solid and liquid MSM for the purpose of determine the better Bacterial isolate which able to biodegradation of pesticides with a concentration of 100 mg / liter of pesticide with a pH 7 and 37 ° C temperature , where the Pseudomonas aeruginosa bacteria proved it efficiency as a bacterial isolate able to analyze Clorspan and Ground up , And for (MIC) experiment the lowest inhibiting concentration of the bacteria growth determined. Since the results indicated that Pseudomonas aeruginosa bacteria continued to grow for all concentrations and both pesticides that start from (50-500) mg / liter. As for Staphylococcus bacteria, the lowest inhibitor concentration was 450 mg/ liter in Clorspan, and for 500 mg / liter for Ground up. As for the ideal conditions, Pseudomonas aeruginosa proved to be the best isolate bacterium which able to analyze pesticides biologically by registering highest values , it recorded value of (95,53,50, 27) in ideal temperature , in 45 ° C for the Clorspan and Ground up pesticides, respectively, and (43.30) During cuddling period for 7 days in clorspan pesticide medium and (39.15) During cuddling period for 8 days in Ground up medium and it recorded (27.87) for pH7 in Clorspan medium. And for Ground up pesticide medium, it recorded a highest values for Staphylococcus bacteria and it was (34.25) at pH7. The study aimed to determine the best bacterial isolate which able of biodegradation of pesticides, and determine MIC (Minimum Inhibition Concentration) for bacterial growth in different pesticide concentrations , Study the ideal conditions for biodegradation of pesticides (temperature, PH, Cuddling period)., Shahad Dhurgham Ali, Lubna Makki Hussein, Ameer MJ Twair
Background: Radiation hazards are harmful and precarious, especially considering professional negligence or ignorance. Objectives: This study aims to assess the knowledge, attitude, and perception (KAP) of undergraduate dental students and interns toward radiation protection protocols. Design: This study is institutional based. Materials and method: The study participants comprised 255 dental undergraduate students and interns, whose curriculum includes dental radiology. Information was collected from each participant through structured questionnaires (18 questions) containing answers in the form of multiple choices. Statistical analysis, frequency distribution and percentage were determined, and chi-square test was performed to evaluate the statistical significance. Results: Among the 255 samples enrolled in the study, 75 were interns and 180 were undergraduates (85 and 95 from third and fourth years, respectively). The overall correct response was 79%, which in descending order was interns (92%) > fourth-year students (86%) > third-year students (60%). Conclusion: The KAP level with regard to radiation protection protocol was highest with interns and the least with the third-year students. This outcome necessitates incessant teaching to ensure maximum safety.
265 ANALYSIS OF CHILDHOOD CANCER RISK BY USING 2 × K CONTINGENCY TABLES WITH VARIETY STATISTICAL METHODS, Mahdi Wahhab Neaman, Bashar Khalid Ali
The main objective of this paper is to analyze the 2× K contingency tables with three statistical methods (regression analysis, multinomial logistic regression analysis and fuzzy model). We compare these methods for evaluating the association between a risk factor and a disease. These statistical methods measure the association between the numeric levels of a risk factor and a disease in different ways. They have been applied to a set of data of childhood cancer risk from prenatal x-ray exposure. Regression and multinomial logistic regression analyses show similar results for a data set of 16226 children whereas the fuzzy analysis yields a different result.
266 ASSESSMENT OF THE RELATIONSHIP OF BASAL SERUM ANTI MULLERIAN HORMONE LEVELS AND MATERNAL AGE WITH PREGNANCY OUT COME IN PATIENTS UNDERGOING ICSI, Hadeel Hassan Jawad, Lubna Amer Al-Anbari, Muayad Sraibet Abbood
The effects of maternal age on pregnancy outcomes are well known, particularly the association of advanced maternal age with poor ART results. Decreased ovarian reserve and decreased endometrial receptivity resulting from increased age are likely reasons for this observed reduction in fertility. AMH plays a role in regulating ovarian activity. Additionally, it inhibits initiation of the development of primordial follicles and the selection of a high number of follicles by decreasing the follicles’ sensitivity to follicle stimulating hormone (FSH). The aim of the study is to assess the relationship of basal serum antimullerian hormone level and maternal age with oocyte quality and embryo quality and pregnancy outcomes in patient undergoing ICSI. The current observational prospective study included 100 infertile women who underwent ICSI at The High Institute of Infertility Diagnosis and Assisted Reproductive Technology/AL-Nahrian University, for the period from September 2018 to June 2019. All patients were treated with a GnRH antagonist protocol. Hormonal assay was performed using ELISA technique (BioTek, USA and CAT# AM448T, CalbiotechInc, CA, USA). At the end of the ICSI cycles 29 women have succeeded to get positive clinical pregnancy, while 71 women unfortunately have failed to get pregnant; thus the clinical pregnancy rate was 29 %. When subfertile women were classified into 4 subgroups, positive pregnancy outcome was significantly highest in women with age ≤ 33 and AMH > 1.63 (73.1 %), followed by women with age ≤ 33 and AMH ≤ 1.63 (21.7 %), then women with age > 33 and AMH ≤ 1.63 (9.1 %) and lastly by women with age > 33 and AMH > 1.63 (8.7 %). Age less than 33 and serum anti-mullerian hormone (AMH) levelof > 1.63 ng/ml are the best predictor of pregnancy in subfertile women undergoing intracytoplasmic sperm injection (ICSI).
267 LARVICIDAL EFFECT OF EXTRACELLULAR SECONDARY METABOLITES OF DIFFERENT FUNGI AGAINST THE MOSQUITO, CULEX MOLESTUS FORSKAL, Aseel Kariem Alsultany, Maysaa Taqi Al-Khazali, Ibtisam A Al-ALi, Maedah Ayyed Khalaf
In this study, the efficacy of eight fungi isolate against four instarlarvae of mosquito vectorCulexmolestusForska were tested.Fungi isolated and identified wereAspergillus niger,A.flavusA.candidus, Penicillium sp., Candida sp, Alternariaalternaria, Fusariumsolaniand Trichophytonviridel.The larvae were treated with different periods ranged from 48to 96 hours. The results showed the presence of a significant effect ofthe fungal filtrates A.niger and Penicillum sp. on the mortality of larvae as the percentage of mortality reached 74.85% and 64.44 %,respectively compared to the rest of the fungi.The concentration of 100% surpassed the remaining examined concentrations yielding the highest percentage of mortality 96.66% and 100% for the fungus A.niger and 68.33% and 100% for the fungus Aspergillus, respectively within 48 and 96 hours after treatment.
268 THE FUNCTION OF MELATONIN HORMONE IN THE REORGANIZATION OF THE IMPACT OF THE OXIDATIVE STRESS INDUCED BY BISPHENOL A IN HYPERLIPIDEMIA ALONG WITH DIABETES IN SERUM OF RATS, Haitham L Abdulhadi, Banan R Dabdoub, Loay H Ali, Azza I Othman, Mohamed A El-Missiry
Environmental pollution had been a reality of life for several centuries however it became a true drawback since the beginning of the commercial revolution. Environmental contaminants are shown to induce ROS generation in each intra- and animate thing areas of cells or people resulting in cell death and tissue injury. Chemicals that induce effects by uncomfortable endocrine systems or mimicking endocrine mediators are jointly delineated as endocrine disrupting compounds (EDCs). Bisphenol A, (BPA) (2, 2-bis (4-hydroxyphenyl) propane) is one in every of the world's highest production volume compounds. It a phenolic compound and is wide utilized in the assembly of resins and polycarbonate plastics. Earlier, it's been shown that BPA induced oxidative stress in numerous tissues in human and rats. This study investigates the results of Bisphenol A pollution on Lipids profile, glucose level, antioxidants and therefore the potential pollution protection by melatonin in experimental. The rats received ten mg/kg of internal secretion pursued by 50 mg/kg of Bisphenol A 3 days per week for 6 incontestable increase triglycerides, total cholesterol, LDL and VLDL, illustrate important in cluster treated with BPA for three and 6 weeks cluster. These were related to the enlarged in lipid peroxidation and cut inhibitor glutathione, superoxide dismutase, enzyme activity and increase in malondialdehyde levels. The protection by internal secretion was determined in connection with the rejuvenation of all oxidative parameters within the blood serum, suggesting that internal secretion is employed for management of many diseases, like avoirdupois, diabetes, cancer, and immune and cardiac disorders. It will recapture ROS by its antioxidant qualities and raise antioxidant enzyme activities.
269 THE PROTECTIVE ROLE OF PHITOFERT AGAINST NAPROXEN-INDUCED TOXICITY IN THE PROSTATE GLAND OF RATS, Hayder Hasan Taher, Nahla JM Al-Shahery
The currentness study was designed to investigate the effects of the treatment with naproxen drug on the physiologicalfunctions "Zinc, prostatic and serum PSA concentration", some biochemical markers "Total protein and GSH, CAT and MDA levels", as well as histometrical and the histopathological aspects of prostate ventral lobe. Also this study examined the phitofert product and selenium in treating of naproxen's effect. Therefore, twenty four adult male rats were divided into 3 groups (8 rats each) as follows Groups 1; rats were administrated with 1 ml of d.w. for 28 successive days. Group 2; rats were administrated with 21 mg.Kg-1 of naproxen for 28 successive days. Groups 3; rats were administrared with 21 mg.Kg-1b.w., and at 5-6 h later they were administrated with 7 mg.Kg-1 b.w of phitofert for 28 successive days. Naproxen-treated rats "Group" exhibited a significant (P≤0.05) declining in the zinc and PSA concentration, PVL total protein, GSH concentration, CAT activity and a high increment in the MDA level that indicated to appearance of oxidative stress status.The naproxen had passive impact on the weight, histometrical and histopathological of glandular "acini" and excretory "ducts" portions. While, the administration of phitofert abrogated all above naproxen induced passive effects.
270 EFFECT OF REST AND STRESS ON SALIVA PH AND DENTAL CARIES, Karama MT Al-Nuaimy
Salivary fluid is an exocrine secretion consisting of approximately 99 percentage water, containing a variety of electrolytes (sodium,potassium,calcium,chloride,magnesium,bicarbonate,phosphate) and proteins, represented by enzymes, immunoglobulins and other antimicrobial factors, mucosal glycoproteins, traces of albumin and some polypeptides and oligopeptides of importance to oral health. Regulation of oral PH is an important function of salivary buffering systems including: the bicarbonate, phosphate, protein-based buffers system and urea. Stress is defined as a state in which homeostasis, as a dynamic balance of internal conditions necessary for the proper functioning of cells or the living organism as a whole is affected by the action of various factors-stressors. This study aim to find the effect of stress on saliva PH among healthy individual and its correlation with caries with incidence. Seventy five undergraduate dental students participated in this study at age (20-25) years. They were examined using DMFT index and asked to provide two samples of unstimulated whole saliva, the first sample at rest, normal circumstances and second after one week applying the Trier Social Stress Test –TSST, saliva PH and viscosity was recorded each time using saliva PH meters. The saliva PH mean at rest (6.90) and in stress (7.40).The P-value was 0.000(less than0.05)which indicates that there is a significant difference between saliva PH mean at rest and saliva PH mean in stress, saliva PH mean of males at rest (which was 7.4 which is the highest)with that of females(which was 6.9).
271 CORRELATION OF ECHOCARDIOGRAPHIC DERIVED SYSTOLIC TIME INTERVALS IN HEART FAILURE PATIENTS WITH OTHER LEFT VENTRICULAR SYSTOLIC INDICES, Ahlam Kadhim Abbood, Zainab F Hassan, Shokry F Alsaad
Despite the advanced echocardiographic techniques in assessment left ventricular systolic function, still there is a need for easy, reproducible and useful methods in difficult conditions. Aim of the study: evaluation the value of systolic time intervals in assessment left ventricular systolic function by comparison with conventional and advanced systolic indices. Total57 patients with heart failure involved in the study, ECG and echocardiographic assessment had been done for them, measurement of systolic time intervals ( pre ejection period (PEP), left ventricular ejection time ( LVET ) and calculation the ratio: PEP / LVET were done.Correlation study were done between PEP and QRS width. AnotherCorrelationwere done between these intervals and the measured stroke volume index (SI), cardiac index (CI ) and global longitudinal strain ( GLS ). Correlation studies of PEP showed statistically significant positive correlation ofQRS width ( r = 0.54, p< 0.05 ), non-significant negative correlation of SI and CI (r = - 0.06, - 0.23, respectively, p> 0.05), and non-significant positive correlation of GLS ( r = 0.12, p > 0.05 ). Correlation study of LVET showed statistically significant positive correlation with SI and CI ( r = 0.91, 0.49, respectively), and statistically significant negative correlation with GLS ( r = - 0.32 ). P < 0.05. Correlation study of PEP/LVET showed statistically significant negative correlation with SI and CI ( r = - 0.54, - 0.45 respectively ), and statistically significantpositive correlation with GLS ( r = 0.36 ).p< 0.05. Correlation studies of PEP showed statistically significant positive correlation ofQRS width ( r = 0.54, p< 0.05 ), non-significant negative correlation of SI and CI (r = - 0.06, - 0.23, respectively, p> 0.05), and non-significant positive correlation of GLS ( r = 0.12, p > 0.05 ). From these correlations it seems possible to get benefit from systolic time intervals in difficult conditions with poor windows and LV systolic assessment is importantas in therapeutic decisionin heart failure patients.
272 DETECTION OF CYTOMEGALOVIRUS (CMV) IN POLY-TRANSFUSED THALASSEMIA PATIENTS AND ITS CORRELATION WITH ABO BLOOD GROUPS, Alaa Younis Mahdy Al-Hamadany, Hadeel Ismael Ibrahim, Safaa M Sharef
Thalassemia is an inherited disorder affecting millions around the word including Iraq. Patients with Thalassemia need repeated transfusion, which might result into number of complications and transmitted many infectious agents including viruses. So this study carried out to show the association between CMV infection and Thalassemia patients. A cohort of 143 serum samples was collected from patients with Thalassemia within the age group of 3-55 years (81 males & 62 females) and 20 from a healthy control. The levels of CMV (IgM) and CMV (IgG) antibodies were measured by Vitek Immunodiagnostic Assay System after performed the ABO blood groups and the Rhesus typing by the standard slide agglutination using commercial antisera. The results showed that the blood groups of the patients with Thalassemia were 37.76% O, 24.48% AB, 19.58% A, 18.1128% B.The results of Rh phenotype tests in patients with Thalassemia patients were Rh+ 81.12 % and Rh – 18.88%.The results of seropostively CMV IgM was recorded with blood groups A, B and AB while the infectionhas been not showed in females with group O while the results of anti-CMV IgG antibodies in relation with blood groups showed 29 seropostively, including 11 (14.09 AU/ml) in blood group AB, 8 (13.237 AU/ml) in blood group B and in blood groups A was 7(12.0 AU/ml) while in blood group O was 2 (12.35 IU/ml). According to gender, the highest titers of CMV IgG antibodies present in females with Thalassemia disease. The positivity of CMV infection did not show any significant correlation withblood groups in thalassemia patients (p>0.05).
273 MOLECULAR DETECTION OF KAPOSI’S SARCOMA ASSOCIATED VIRUS (HUMAN HERPES VIRUS 8) IN SINO-NASAL CARCINOMA TISSUES, Hind Hamed Shaker, Luma Amer Yasin, Ausama Abed-Alkadum Alajeely, Saad Hasan Mohammed Ali, Shakir H Mohammed Al-Alwany
Sinonasal malignant neoplasms are rare and heterogeneous tumors of sinonasal tract, constituting about 3% of the upper respiratory tract tumors. A variety of gene products encoded by Human herpes Virus – 8,are involved in transformation, as well as promotion of oncogenesis. angiogenesis, anti-apoptosis, and immune modulation and evasion. The aim is to unravel the ratesof Human herpes Virus – 8 (HHV-8) infections in tissues from a group of patients with sinonasal carcinoma (SNC) and sinonasal papilloma (SNP). Eighty- three formalin-fixed, paraffin- embedded sinonasal carcinoma (SNC) and sinonasal papilloma (SNP)tissues enrolled in this study; 18 sinonasal tissue biopsies from sinonasal carcinoma; 35 tissue biopsies from sinonasal papilloma and 30 apparently healthy nasopharyngeal tissues with unremarkable pathological changes, as control group. Detection of HHV-8 was done by new detection version of chromogenic in situ hybridization (CISH) technique. The positive CISH reactions for HHV-8-DNA were detected in 22.2% and 5.7% of sinonasal carcinoma and sinonasal papilloma tissues, respectively. And no control tissues presented positive signals for HHV-8-CISH test. The correlation between HHV-8 and sinonasal carcinoma and sinonasal papilloma was highly significant (P= 0.001). The significant HHV-8 detection rates in sinonasal carcinoma and sinonasal papilloma tissues might shade light for a possible role of this virus in either sinonasal pathogenesis or carcinogenesis.
274 CLINICAL AND OXIDATIVE STRESS LEVELS IN PATIENTS WITH MORBID OBESITY AFTER 6 MONTHS FOLLOW UP POST MINI-GASTRIC BYPASS OPERATION, Ghaith Sael Abu Naila, Maryam Qais Ahmad Alqaisi, Ali S Musa Al Shammaa
Obesity to increase fatty tissue, it is associated with a lot of medical and chemical changes imagining the illness of multiple body organs, when decrease body weight lead to decrease formation of reactive O2 cause tissue harm. So the aim of this study is to evaluate the changing in oxidant as well as antioxidant before and afterbariatric operation in obese patients. The current study was a prospective-controlled clinical study, the study involved 184 individuals: control group consisted of 92 subjects and Bariatric group consisted of 92 obese individuals, Patients studied were admitted "between January 2018and April 2019". In current study we assess the degree of OS in patients with morbid obesity > 38.5 kg/m2 at basic state and its reduction six month after bariatric surgery using the mini-gastric bypass method. When match the normal and obese patients, there is significant increase in lipid peroxidation when increase in level of 8-epi-PGF2 in morbid obesity patients. The 8-epi-PGF2 is perfect marker for checking of oxidative stress in patients with morbid obesity.
275 PENTRAXIN3 AND NITRIC OXIDE-ASSOCIATED WITH AN ATHEROGENIC INDEX AND TYPE II DIABETES MELLITUS, Suhayla K Mohammed, Ekhlass M Taha, Samer Abdulhassan Muhi
This study concentrated on evaluation pentraxin3, as well as Nitric oxide and their correlation with atherogenic index (AI) in Diabetes mellitus II. The current study includes 30 control subjects(group I), and60 Iraqi patients with type II diabetes mellitus(T2DM).The patient group was subdivided in to two groups: group II (30) Newly diagnosed T2DM and group III(30) T2DM (how were treating with metformin monotherapy). In the present study pentraxin3, Nitric oxide was measured by enzyme linked immune sorbent assay (ELISA). Biochemical parameters, fasting serum glucose, insulin, HbA1c,and lipid profile were measured through spectrophotometer technique in patients and control groups. The current study showed significant increase in levels of FSG, HbA1c, BMI, WHtR, TC, TG, LDL, VLDL while HDL level was decrease in Newly diagnosed DM, whereas insulin appeared non-significant deference. A high significant increase in Nitric oxidelevels in diabetic with metformin therapygroup, significantincrease in pentraxin3innewly diagnosed diabetic group.Positive significantly correlation coefficient between pentraxin 3 and HbA1c.,atherogenic index and negative significantly correlation with HDL, while there was no significant correlation between pentraxin 3 and nitricoxide. Effect of gender was significant of Nitric oxide inNewly diagnosed diabetic group and control group, whilepentraxin3 showednon significant effect of gender in all studied groups. Atherogenic index positively correlated with pentraxin3.Metformin therapy increasenitric oxide significantly, while non-significant effect was shown with pentraxin3.
276 IMMUNOLOGICAL, PHYSIOLOGICAL, ANDBIOCHEMICAL STUDY OF SOME PATIENTS WITH RHEUMATOID ARTHRITIS IN KIRKUK CITY, Mohanad Hasan Mahmood Al-Izzi, Marwa Abd-Alsalam Qadir Al-Hashim, Firas Faris Riga
This study was designed in order to estimate some immunological (IL-6 and C-Reactive Protein –CRP-), physiological (Resistin) and biochemical (Glutathione and Malondialdehyde) parameters of some patients with rheumatoid arthritis in Kirkuk city. The study involved (60) blood samples were divided into 2 groups: group one: adult normal healthy individuals as control group, group two: adult patients with rheumatoid arthritis. The results of this study found significant increase inpro-inflammatory cytokine IL-6 concentrations at levels (P≤ 0.05) of second group in comparison with control group ; and there are significant increases in adipokineresistinconcentrations at levels (P≤ 0.05) of second group in comparison with control group; and there are significant increases in CRP concentrations at levels (P≤ 0.05) of second group in comparison with control group and there are significant decreases in glutathione (GSH) concentrations at levels (P≤ 0.05) of second group in comparison with control group, and there are significant increases in malondialdehyde (MDA) concentrations at levels (P≤ 0.05) of second group in comparison with control group.
277 THE ROLE OF ENOSGENE POLYMORPHISM IN THE RISK OF ASTHENOZOOSPERMIA INCIDENCE IN A SAMPLE OF IRAQI PATIENTS, Istikrar M Hade, Ismail A Abdul-Hassan
Single nucleotide polymorphisms are considered as one of causes of male infertility. The eNOS is primarily responsible that create of NO in the vascular endothelium, therefore eNOS is mainly expressed in thehuman testicular endothelial cells, in addition of Sertoli cells and Leydig, epididymis and vas deferens which regulates the synthesis of NO. Many study informed in the first time about the NO that is synthesized by human male gamete. Experimental evidence has shown that NO in high concentrations can cause defective function of the sperm and low concentrations of NO play very important rolein the control of the sperm physiology. In this study, the relationship of eNOS polymorphism (rs1799983 T>A)in exon 8, was studied in 50 asthenozoospermic patients and 50 fertile men. Analysis of these SNPs was performed using real time thermocycler(Real-time PCR). As related with eNOS genes rs1799983T>A SNP, TT (homozygous), no significant differences in frequency percentage were noted between fertile men subjects and asthenozoospermia patients Whereas, the frequency of heterozygous TA genotype in asthenozoospermia patients are higher than fertile men group, but showed no risk factor.As related withAA (homozygous) showed a risk factor to cause infertility.
278 STUDY THE EFFICACY OF TITANIUM OXIDE (TIO2) NANOPARTICLES AGAINST PROTOSCOLICES OF HYDATID CYSTS, Amenah Abdul-Jabbar Ibrahim
Hydatid cyst disease is one of the most common diseases in many places in the world. The infection occurs when human and livestock drinking or eating contaminated water and food with eggs of Echinococcus granulosus worm. Surgery is the best solution to eradicate cysts and rapid healing, but it may be accompanied by some risks such as rupture of the cyst and leakage its contents of protoscolices, which leads to the return of infection and spread in the body. Several methods have been used to reduce the risks of surgery, including withdrawal of hydatid fluid and its contents and injection scolicidal substances like ethanol and others. Researchers have recently tested the efficiency of nanoparticles such as selenium, silver, and gold nanoparticles against the protoscolices. The present study aims to test the efficiency of Titanium oxide (TiO2) nanoparticles versus protoscolices of hydatid cyst. Protoscolices were obtained from hydatid cysts in sheeplivers. The TiO2nanoparticles were prepared at concentrations of 250, 500, 1000, 2000, and 4000 µg/ml at different times (15, 30, 60 min.). The results showed the efficacy of all concentrations at all times in the killing of protoscolices compared with control groups.
279 ASSOCIATION OF LEVELS OF CALCIUM (CA++) AND URIC ACID (UA) LEVELS WITH OSTEOARTHRITIS (OA) IN IRAQI PATIENTS, Moazaz Rashad Saed, Mohammed Imran Hamzah, Muhammed Abdul-Gabar Hamoodi Aldabagh, Hadeel Sameer Abd Alwahab
Osteoarthritis is a prevalent degenerative deficiency of the articular cartilage linkedwith hypertrophic bone changes. Present study was designed to examine effect serum levels of Ca++ and UA in development of OA in Iraqi patients. Total of 75 subjects were enrolled in current study, 50 patients with OA and 25 healthy subjects controls. Serum levels of Ca++ and UA were measured in each volunteer. Current study showed significant elevation (p value< 0.05) in UA and nonsignificant differences in Ca++ compared to controls group. Our results revealed no effects to serum Ca++, while UA may has important roll in emergence of OA in Iraqi patients.
280 Anxiety, depression, and suicide among adolescents in Al-Shirqat city, Hassan Hussein Saleh, Basim Hussein Bahir
Many mental health disorders like anxiety, depression, and suicide emerge in late childhood and early adolescence and contribute to the burden of these disorders among young people later in life. It is common worldwide but often unrecognized. This study aims to identify the anxiety, depression and suicidal behavior that affect adolescents in Al-Shirqat city. A cross-sectional study was conducted among 840 early and middle adolescents (10-17 years) in Al-Shirqat city, during the period from May to October 2019. A structured questionnaire was used to assess GAD-7 Anxiety, PHQ-9 Depression and Suicide Behaviors Questionnaire-Revised SBQ-R. The prevalence of severe anxiety and severe depressions were 1.3% and 3.9%, respectively. Suicide behaviors found in 14% of adolescents. There were 2.4% of adolescents likely attempt suicides someday. In conclusion, the current study provides significant information on psychological variables related to adolescent’s health risk behavior.
281 Evaluating the radioactive contaminations of soil samples in some schools of Mosul province: a health detection, Ali Salman Hassan, Mushtaq Ali Husseinv, Shaymaa Awad Kadhim alshebly, Hayder H Hussein
In this study, we will investigate the radiation activity of 20 soil samples from five schools in Mosul Province were measured the gamma spectrum by using NaI (TI) Discovery which has dimensions 3*3 crystal. The activity concentrations of naturally occurring of 40K ranged from (310.414±3.40 to 1359.220±1.37) Bqkg−1, 238U reached from (1.035±0.300 to 62.636±0.108) Bq kg−1 and 232Th ranged from (0.583±0.060 to 24.139±0.47) Bq kg−1while the average activity concentration observed 40K was 936.340 ± 3.88 Bqkg−1, 238U was 18.861±0.391 Bqkg−1and 232Th was 5.376 ± 0.312Bqkg−1. The radiological hazard parameters (representative level index, radium equivalent activity, outdoor and indoor dose rates, outdoor and indoor annual effective dose equivalents, and radiation hazard index) were calculated from the radioactivity in the soil. Results of the present work concerning values of radium equivalent activity, outdoor annual effective dose rates, the gamma index, and internal and external hazard indices, all were found to be lower than their corresponding allowed limits given by the world average. The data produced in the present work can be used as baseline radiological data for future investigations.
282 The therapeutic effects of ambrisentan on experimentally induced colitis in a male rat's models, Rana K Atarbashe, Ahmed Abu-Raghif
Ulcerative colitis is a chronic, intermittent illness. The current treatment failed to cure the disease which made a need to investigate another drug with minimal side effects. The present research was conducted to assess the antioxidant and anti-inflammatory effects of ambrisentan in comparison with that of sulfasalazine in experimental colitis in rats. Acetic acid 4% (vol/vol) was used rectally to induce experimental colitis in rats. After induction, rats were administered either sulfasalazine 100mg/kg or ambrisentan 5 mg/kg orally for one week. There was the estimation of histopathological and macroscopical parameters also the expression of cytokines (TNF-α and IL-4), oxidative stress markers, malondialdehyde (MDA) and myeloperoxidase (MPO), and adhesion molecules (ICAM-1and E-Selectin) in the colonic tissue. Both ambrisentan and sulfasalazine significantly reduced the macroscopical and histological injury in the colon induced by acetic acid. In addition to the downregulation of the increased colonic proinflammatory cytokines, MDA, MPO parameters and adhesive molecules. In conclusion: Ambrisentan had an effective role in experimental colitis in rats through anti-inflammatory and antioxidant actions with downregulation of the colonic adhesive molecule.
283 Microdebrider-assisted turbinoplasty versus submucosal diathermy in treatment of inferior turbinate hypertrophy, Ahmed Muhei Rasheed, Hatem A Hatem, Nibras Jassam Homadi
The surgical treatment of inferior turbinate hypertrophy (ITH) is challenging. Submucosal diathermy (SMD) is a well-known surgical procedure used for the treatment of ITH, microdebrider-assisted rhinoplasty (MAT) is relatively a newer technique used in the management of ITH. To evaluate the effect of MAT on inferior turbinate size and nasal airway patency in patients with bilateral ITH in comparison to SMD. Seventy-one patients presented with nasal obstruction due to bilateral ITH were allocated into two groups, group a (35 patients) were subjected to SMD and 36 patients in group B were subjected to MAT. All the patients had been sent to CT scan pre-operatively and at the third postoperative month to measure the cross-sectional areas of the inferior turbinate, also the subjective assessment of nasal airway patency was done by visual analog scale (VAS) preoperatively and at the end of 3ed post-operative month. The mean cross-sectional area of IT decreased from 163 mm2 pre-operatively to 149.67 mm2 at the 3ed post-operative month in group A (P-value =0.02), and from 163.67 mm2 to 131mm2 in group B (P value= 0.004). Intergroup comparison had shown that there was a significant difference between MAT and SMD in decreasing the size of IT and relieving nasal obstruction at the 3ed postoperative month (p-value = 0.026 and 0.021 respectively). In conclusion, both MAT and SMD are effectively decreasing the size of the inferior turbinate and relieving nasal obstruction but with superior results in MAT.
284 Study of some of Aeromonas hydrophila virulence factors and antibodies production in laboratory mice, Nuha A Hameed Al-Mojamaee, Hiyam Adel J Al-Taee
The current study deals with the extraction and preparation of the bacterial antigens of Aeromonashydrophila taken from wound which were identified depending on morphological and biochemicalcharacteristics,and these antigens have included Whole Cell Antigens (WCA), Lipopolysaccharide( LPS), and Pili antigens,alsoThin Layer Chromatography (TLC) for LPS and Pili antigens were used to determine the presence and purity of the antigens, and theresults have showed the presence and purity of the antigens in the prepared extracts. The ability of the extracted antigens to stimulate the immune system in the laboratory mice to produce antibodieshave been studied, (10) BALB/c mice were used and divided into (5) groups each group was immunized with one of extracted antigens except the control group which has been injected with (0.2) ml of phosphate buffer saline, and the presence of antibodies in immunized mice sera were determined by using various immunological methods included: Slide Agglutination Test (SAT), Enzyme Linked Immunosorbent Assay (ELISA) and Counter Immunoelectrophoresis (CIEP). The result of immunological methods showed that all theA.hydrophila antigens stimulate the immune response in immunized laboratory mice by producing the antibodies.Also in the present study ELISA and CIEP methods showed the best results, in which have given a positive result of the presence of anti-antigen antibodies in all immunized laboratory mice compared with the SAT method, LPS antigens and the whole cell antigens of A.hydrophila were more stimulating and had a higher immune response in immunized laboratory mice,in comparison with the Pili antigen which have shown the least percentage of antibodies in immunized mice sera.
285 Assessment of linear parameters of Electrohysterograph (EHG) in diagnosis of true labor, Farah Fatehalla Shero, Ghassam Thabet Saeed Al-Ani, Ekhlas Jabbar Khadim, Hind Zuhair Khaleel
The diagnosis of true labor has been described as one of the most difficult and important judgments made by providers of maternity care as accurate diagnosis of true labor is essential to predict preterm birth as early as possible to prevent subsequent complications associated with prematurity. Currently used techniques for prediction of true labor are either highly invasive (IUCP) or highly subjective with low predictive values (TOCO). There is a great need for a more reliable and non-invasive method to detect electrical changes in uterine muscles that occur during the transition from pregnancy towards labor. Analyzing EHG records and some linear parameters extracted from these records among Iraqi women during pregnancy and delivery. Pregnant ladies who attended the obstetrical wards and labor rooms of Baghdad Teaching Hospital and Private Nursing Home at Baghdad Medical City from April- July 2019 and met the inclusion criteria were enrolled and divided into laboring and non-laboring groups based on presence of labor signs. EHG was recorded for 30 minutes using 4 disposable Ag/AgCl electrodes attached to the abdominal surface and a reference electrode. EHG records were digitized, stored and then analyzed to extract and compare some linear parameters between the two groups. Thirty-two pregnant ladies with a mean age of (27.72±5.64 years) were included in the study then were subdivided into 18 laboring and 14 non-laboring matched groups. Results showed a significantly higher root mean square, mean and peak frequency of the power spectral density values in labor compared to non-labor groups. In conclusion, Uterine EHG monitoring in pregnant women can be a very promising tool to diagnose the onset of true labor. Linear features extracted from EHG record, i.e. root mean square, peak and mean frequency of the power spectral density increase significantly in labor than pregnancy groups.
286 Role of antiGAD65 Ab., C-peptide level and clinical characteristics in classification of newly diagnosed diabetes in patients aged 20-40 years, Nihad Abdallah Selman, ALi Hussein Alwan Albayati, Yasameen Alsaffar
Classification of newly diagnosed diabetes in patients aged 20-40years and the further decision to treatment may be difficult depending on clinical manifestation alone. The purpose of the study to identify the role of specific tests (antiGAD65 Ab, and level of C-peptide) and their relation to clinical characteristics in the classification of diabetes. One hundred newly diagnosed diabetes patients aged 20-40years enrolled in this study between Jan.2013-Dec.2017.The parameters of age, gender, history of osmotic symptoms, ketoacidosis, SBP & DBP measurement height, weight, BMI, were taken. C-peptide level and (anti-GAD65Ab) were conducted to all patients. While FBS and 2 hours post-GTT and HbA1c conducted at first visit and after 3 months. In patients with positive results of anti-GAD65Ab, there was significant low level of C-peptide (P
287 Menstrual disorder: Cross-sectional study on prevalence and self-care practice among adolescents in Iraq, Harith Kh Al-Qazaz, Raghad O Al-Dabbagh
and management practice of menstrual disorder in Iraq. Finding prevalence and pattern of menstruation disorder as well as the self-care activities among adolescent girls in Mosul city, Iraq. A descriptive cross-sectional study was carried out among 385 single females in secondary schools in Mosul city with an age range of 14-18 years from October to December 2018. Ten randomly selected schools were invited via an official letter from the research group and only those agreed to be included were visited. A semi-structured questionnaire was prepared by the researchers and the questionnaire was systematically translated and validated to produce the Arabic version. The mean age was 16.11 years ± 0.787. The age of menarche of 77.9% of the participants was between 13–15 years. The prevalence of dysmenorrhea was 93.6% and the prevalence of premenstrual symptoms was 84.9% among the study sample. Mothers’ level of education, family size, the experience of PMS and regularity of the cycle were significantly associated with the history of having dysmenorrhea. In conclusion, the prevalence of menstrual disorders, including dysmenorrhea and PMS, among Iraqi adolescents was high and affect the social and academic performance and even low-grade scoring of the students. The study highlights the need for the menstrual educational systems in the schools, in addition to screening activities performed by health care providers from school health sectors in the directorates of health.
288 The activity of camel milk to treated immunity changes that induced by Giardia lamblia in male rats , Abeer Abbas Ali, Shahad Abbas Ali Al-Attar
The current study was aimed to detect the activity of camel milk to treat the immunity changes induced by G. lamblia. 20 male rats using and divided into four groups (5 rats in each group); control group that received normal saline, second group rat injected intraperitoneally with G. lamblia at dose 7X107 cyst/ml. third group rat injected intraperitonealG. lamblia at dose 7X107 cyst/ml and treated with 0.5ml of camel milk for 2 weeks. Fourth group: rat injected intraperitoneally with G. lamblia at dose 7X107 cyst/ml and treated with 1ml of camel milk for 2 weeks. The results, after two weeks, show significant changes (P < 0.05) in concentrations of IL-2, IL-6, and IL-8 in the group that injected with G. lamblia compared with the control group. The results, after four weeks, demonstrated high significant changes (P < 0.05) in concentrations of IL-2, IL-6, and IL-8 in the group that injected with G. lamblia compared with the control group. After using camel milk, concentrations of IL-2, IL-6, and IL-8 in the group are treated groups (after two and four weeks) demonstrated non-significant changes (P < 0.05) with the control group. It was concluded that camel milk possesses activity as an immunity enhancer.
289 Quantification of C-type lectin gene expression during hyperinfection in strongyloidiasis, Khalid JK AL-Zihiry, Noor Abdulhaleem, Salman S Atshan, Amal J Kadhim, Zaid O Ibraheem, Ngah Z Unyah
Strongyloides stercoralis is the intestinal nematode in humans, and it infects millions of people worldwide but thrives in warm countries with poor sanitation conditions. Clinical manifestations of the infection may range from asymptomatic to chronic. Strongyloides cause hyperinfection syndrome and dissemination in individuals with impaired cell-mediated immunity due to its ability to proliferate within the host that may increase the mortality rate up to 87%. The diagnosis of hyperinfection syndrome is difficult to establish and entails a high level of suspicion. The objective of the present study was to measure the expression level of C-type lectin gene coding to protein biomarker candidates from the excretory/secretory (ES) products of the infective filariform larva that can be used as diagnostic indicators for early hyperinfection syndrome in strongyloidiasis. An experimental study was carried out to induce hyperinfection of L3 larvae of S. ratti in experimentally immunosuppressed Wistar rats using prednisolone, a corticosteroid immunosuppressive drug. Prednisolone treatment resulted in a significant increase in the parasitic intensities. Relative semi-quantitative real-time PCR was performed to compare the expression level of the C-type lectin’s gene between treated and non-treated groups with this drug. C-type lectin gene showed significantly higher expression levels in the treated samples. The study concluded that C-type lectin expression level was successfully measured and could be used as a diagnostic biomarker during early hyperinfection syndrome in strongyloidiasis.
290 Estimating the difference of glucose levels during 1st and 3rd trimester of pregnancy in Tikrit city in Iraq, Mayada Kamel Mohammed
The occurrence of hyperglycemia among pregnant ladies who visit Salah Aladdin general hospital is examined in relation to age and no. of pregnancy to determine the occurrence of diabetes mellitus in pregnancy. Blood glucose levels were evaluated in the first and third trimesters of pregnant women by the glucose-oxidase principle using the Easy Touch® G blood glucose monitoring system. This study is a cross-sectional study, carried out in the Salah Aladdin general hospital, from the 15th of November 2018 to the 10th of March 2019. A total of 120 women ages ranged from (20 -45 years) have been selected. By using 14 direct factors questionnaire affecting pregnant women in the first and third trimester. Statistical analysis of data was done by manual statistics. The serum glucose levels elevated markedly in the third trimester of pregnancy and the glucose level not so increase in the first trimester. This raising in serum glucose level in the third trimester may lead the pregnant to hyperglycemia or diabetes mellitus of pregnancy. Over the majority of pregnant women are free from GDM 86.67% while 13.33% having GDM where 87.5% are in the third trimester and 12.5% in the first trimester of pregnancy. To conclude percentage % of first/third trimester of pregnancy having GDM are 12.5% 1st trimester. 87.5% 3rd trimester. Percentage % of pregnant women 13.33% have GDM while 86.67% are not having GDM Ranges of blood glucose level in first trimester 88 while in third trimester 100.The Aim of study was to estimate serum blood glucose level during 1st and 3rd trimester of pregnancy in Tikrit.
291 Percentage of Creative Thinking Contribution to Meditators versus Impulse in Decision-making Accuracy for First-class Referees in Volleyball, Hanan Adnan Aboob, Basma Naeem Mohsin, Ahmed Aqeel Abbas
The world has seen a rapid development recently in different areas of life, which put the countries of the world in a struggle to reach the best levels between the world countries, this development had not been by chance, but was result of the great and continuous efforts of scientists, experts, the expertise and abilities owners and creators in developing the best studies and researches that helped to discover the state of the art scientific and innovative means in the life branches generally, especially the sports field, which includes several fields, including: education, arbitration, and training.
292 Effect of Isotretinoin on Malondialdehyde (MDA) Level in Iraqi Patient Afflicted with Acne Vulgaris In Baghdad Governorate, Ola Kamal, Zuhair I Almashhadani
Background: The predominantly typical dermatological ailment is Acne Vulgaris. Even though isotretinoin (13.cis. retinoic acid) trade name AAccutane is the drug commonly used to treat Acne Vulgaris and is a well-tolerated drug, like most treatments, it has a wide range of side effects. Objective: The current research objective is in the exploration of the impact of this drug on MDA malondialdehyde level that is the resultant outcome of lipid peroxidation and the level of cholesterol and Triglycerides. Patients and Methods: The patients are those who attended the Dermatology Consultation Clinic in the Medical City Hhospital where the investigation is being conducted on the before and after treatment, with Isotretinoin being taken for a duration of two months. We measured Malondialdehyde (MDA) level and cholesterol triglyceride levels. Results: There is an increase in Malondialdehyde (MDA) level after the treatment with Isotretinoin p value < 0.001, there is also an increase in cholesterol and triglyceride level after the treatment with a p value
293 Hematological study to evaluate the Cyprinus carpio fish healthy in two areas of Baghdad city, Asmaa SI Al-Khayat
The current research was conducted toevaluate thehealth status of common carp fish(Cyprinus carpio) which are sold in the local markets of Baghdad city (ALTaji, AL Rasheed sell market)to demonstrate its suitability for human consumption , where fish samples were takenover two months (January and February 2017)for hematological tests : count of micronuclei in erythrocyte , percentage of leukocytes types (Lymphocyte , Eosinophil , Neutrophil , Basophil , Monocyte and Thrombocyte ).The results of the current study showed that the common carp fish from AL Rasheed sell market have better health compared to fish that was taken from ALTaji sell market and therefore its suitability for human consumption.
294 Behavioural Risk-Factors Associated with Dentists Diagnosis of Oral Cancer, Zahraa Mohammed Hussien Al-Shammary
The study aims to know the importance of early diagnosis of oral-cancer that improves the diagnosis, but dangerous tumour is regularly recognized in progressed stages, when more forceful medicines are required, and the comes about are frequently awful and damaging to the understanding. Oral-cancer can be recognized by deft examination of the verbal mucosa without the required for advanced gear. Dental practitioners are vital in essential and auxiliary anticipation of verbal cancer. Evaluating their information, viewpoints and hones is subsequently basic. Materials and Methods: The study followed the use of a survey via a questionnaire for dentists to identify risk factors, models of oral-cancer diagnosis, major and minor prevention practices, and the effectiveness of proper university training for early exposure and disease avoidance. Results: Dentists seem to have a general-knowledge of diagnostic concepts and risk factors. 89% reported the provision of a soft intraoral oral examination for adult patients to rule out oral- cancer. With A total of 100% always provides advice to stop the use of tobacco. Less than 16% of young dentists between the ages of 25 and 35 reported a decrease in-knowledge of risk-factors compared with dentists in the 36-40 age group (35% while dentists over 40 years were 32%) Of dentists who graduated more than 10 years ago, there are 50% high marks in the-knowledge of risk-factors for dentists who graduated from 1 to 5 years before 12% and graduated from 6 to 10 years before 30%. 23% of dentists described that they would biopsy suspicious lesions. Conclusion: The comes about of the overview demonstrate that dental practitioners are not enough utilized in oral-cancer avoidance and early location, which one of the deterrents is need of preparing. Dentists'-knowledge and abilities ought to be fortified and routinely upgraded through proceeding proficient instruction. More accentuation ought to be put on the reality that dental practitioners have a greater part within the anticipation and discovery of these threatening tumours in their early treatable stages.
295 Direct and Deferred Retrieval of Basketball for Student of the College of Physical Education and Sport Sciences, Oamr Fadhil Yahya Al-Badri, Abedlrahman Ibrahim, Rafid Hussein Mohammed Alani
This study aimed to identify the differences between the members of the sample in direct retrieval and postponed retrieval at the levels (remembering, understanding) according to the variable of the study type (morning and evening) and the acceptance channel variable (scientific, literary, and professional), and the researcher used the descriptive approach to its suitability of the current study and was chosen The sample intentionally from the second stage students for the morning and evening studies at the College of Physical Education and Sports Science / University of Samarra for the academic year 2017/2018 because the basketball course is taught within the vocabulary of the second stage in the College of Physical Education and Sports Science and the sample consisted of He urged (31) students were 77.5% of formed from the research community of 40 students. Tis study comes out with the following results: There are non-significant differences between the students of the study according to the scientific, literary and professional branches in the direct retrieval of the level of remembering, whether between the mornings or evening study students.
296 The Prevalence of Pinworm, Incontinence, and Appendicitis in Children in Diwaniyah Governorate, Iraq, Manar HM Al-Ibrahimi, Ali BM Al-Waaly
The study was conducted during the period between July 2018 and until February 2019 were examined (419) random stool samples for children aged (1-12 years). It included a number of schools and kindergartens in each of the city center, and different areas of its surroundings province of Diwaniya and for both sexes. The results of the study showed that the incidence infected rates were 43.67%.As for the relationship between E. vermicularis and appendicitis, 109 surgically removed appendicitis were collected from Women and Children hospital and Diwaniya Teaching Hospital in Al Diwaniya Governorate. The results of the study showed a relationship between pinworm parasites and appendicitis by 11.92% in each Males and females depending on the examination of the contents of the appendix waste.The results of the examination of 419 children under the age of 12 years for the detection of E. vermicularis and study the relationship of the incidence of urinary incontinence in children. The results showed a relationship between the parasite E. vermicularis and the phenomenon of night incontinence by 66.66% for both sexes. The results of the study also showed a relationship between the incidence of night incontinence and the presence of pinworms with age and sex. The results of the statistical analysis confirmed a positive correlation between the phenomenon of night incontinence with the presence of the parasite E. vermicularis at the probability level P
297 Effect of the Training of Sakio In Some Physiological and Biochemical Variables According to The Lines of Play for The Players of Wasit University Football Team, Fadhi Daham Mansour, Mohammed Kazem Arab, Ahmed Murtada Abdulhussein
The basic physical abilities that characterize performance of soccer players demonstrated their level of quantitative and qualitative performance of virtual players, no matter how different levels of performance depends essentially on the sports body's energy systems because the direction and quality of training depends on the method and system Energy supply base, and as the game of football game with various requirements according to each player's duties or status of the problem was the inability of trainers use a variety of exercises related to the nature and performance of duty required by lines of play than lose the players ability to perform their duties as Appropriate to the nature center play and this caused a clear divergence in the level of the overall performance of the team, so the researchers considered using this new technology in training. A sample of Wasit University football players football applied them playing lines sakiodrills and hammer drills used int to positive "impact on the level of research sample members. The researcher recommended that need attention to aerobic workouts and anaerobic training programming commensurate with performance requirements for playing lines and boundaries.
298 Detection of antimicrobial susceptibility patterns of bacterial species isolated from burns and wounds infections in Basrah Hospitals, Ghosoon Al-Kanaany
Burn and wound infection is one of the most frequent serious sicknesses caused by pathogen, chiefly by both gram positive and gram negative bacteria. This study was carried out to identify 25 bacteria isolated from burns and wounds surgical belong to the species : (Staphylococcus aureus, Staphylococcus lentus ,Staphylococcus intermedius,Staphylococcus lugdunensis, Pseudomonas aeruginosa, Pseudomonas oryzihabitans, Pantoea spp., E.coli, Rhizobium radiobacter, Ochrobactrum anthropic, Burkholdria cepacia, Sphingomonas pauci- mobilis, Klebsiella pneumoniae, Aeromonas sobria.. Staphylococcus 9(36%) was the most dominant organism, followed by Pseudomonas 6(24%).Antibacterial activity of some antibiotics was investigated against bacterial isolates . the data demonstrate the most commoly isolated from burns and wounds surgical were Staphylococcus followed by Pseudomonas .All bacterial isolates revealed high resistance to antibiotics were usеd, Whereas Staphylococcus aureus revealed resistance for three antibiotic: Amikacin ,Nitazo- xanide, Neomycin. Pseudomonas aeruginosa revealed resistance for four antibiotic: Neomycin, Amoxicillin/Clavulanic acid, Cepha- lothin, Cefotaxime. Pantoea spp revealed resistance for five antibiotics: Neomycin, Trimethoprime, Amoxicillin/Clavulanic acid, Cephalothin, Cefotaxime . E.coli revealed resistance for four antibiotics: Neomycin, Trimethoprime, Amikacin, Cephalothin.
299 Effect of genetic polymorphism MHFR C677T in some biochemical markers in a group of βT patients, Wagdi S. Sadeq, Zubaida A Kudaier, Ali N Sabeeh, Faik I Ali
The current study was conducted as an attempt to know about the prevalence of the mutant genotype TT, caused by substitution mutation C – T, in MTHFR C677T and the effect of resulted genetic polymorphism in the levels of hyc in Iraqi patients of βT registered in Kirkuk public hospital, Kirkuk- Iraq, in addition to a try to reveal if any change have been happened in biochemical parameters in the group of patients compared to healthy control group. The number of patients is 50, 25 males and 25 females, the mean of age and standard deviation 8.32 ± 4.41, and 45 of the healthy control group, 20 males and 25 females, with age, mean and standard deviation 9.51 ± 3.41. The percentage of related parents is 38% in the group of patients, while it is 51.1% in the control group. Data of four of patients and seven of healthy individuals were excluded due to the failing of getting amplified output of blood DNA. Electrophoresis of PCR products after incubation with restriction enzyme Hinfl, showed 198bp band which belong to the normal allele C, and two bands of 175bp and 23bp which belong to mutant allele T. Calculation of allele frequency showed increase in the mutant allele in the group of patients which reached 0.368 compared with 0.046 in healthy control group. On the other hand, the results showed a higher frequency of homozygous genotype of mutant allele TT in the group of patients 10.86% compared to 2.32% in the healthy control group. The test of Chi2 showed a significant difference in numbers of observed and expected genotypes, calculated Chi2 value 0.66 compared to tabulated value 5.99 with 2 degrees of freedom and 0.05 level of significance, while it reached 9.84 for the control group. The results of the biochemical study showed no significant differences in levels of hyc between patients and healthy individuals. The value of mean difference and standard error mean reached (2.33 ± 1.56, t= 1.49), the levels of folate reached (1.31 ± 1.05, t= 1.24. While there were significant differences in the levels of ferritin (-387.18 ± 16.79, t= 23.05) and VB12 (202.99 ± 14.49, t= 14.0).
300 ROLE OF Rosmarinus officinalis PHENOLIC COMPOUNDS IN TREATMENT OF Entamoeba histolytica INFECTION, Adwa F. Jassim, Yaseen MS Al-Adilee, Aseel A Mustafa
The present study was designed to detect role of phenolic compounds against the E. histolytica. The study used 20 adult male rats that distributed to four groups (each group consist 5 rats); control group that received normal saline, second group rat administrated with E. histolytica at dose 103cyst/ml. third group rat administrated with E. histolytica at dose 103cyst/ml and treated with 50ug/ml of phenolic compoundsfor four weeks. Fourth group rat administrated with E. histolytica at dose 103cyst/ml and treated with 100ug/ml of phenolic compoundsfor four weeks. The results show high significant increased (P < 0.05) in levels AST, ALT and MDA with high significant decreased (P < 0.05) in levels of catalase in second group compared with control group. The results of third and fourth groups show non-significant changes (P < 0.05) in all parameters compare with control group when using phenolic compounds. About the histological changes, second group show degeneration of hepatocytes with thickening wall of central vein and infiltration of mono-nucleated inflammatory. After treatment by using phenolic compounds, tissues of liver appear semi-normal in third and fourth groups. It was concluded that phenolic compounds has arole against E. histolytica.
301 STUDY OF THE CALCIUM ION LEVELS IN HYPOTHYROIDISM'S WOMEN IN KARBALA PROVINCE, Zahraa Ch Hameed, Aiyat Alshugary, Naseer Abdul Ameer
This research included the study of thyroid hormones activities with calcium ion levels, in women with hypothyroidism in the province of Karbala. To achieve this aim 100 samples were arranged in two groups,60 samples were patient women with hypothyroidism attending Imam Hussein medical city, and (40) healthy womenas control.The results appear decrease in concentration of (T3) and (T4). While the results appear increased in concentration of(TSH), and appear no differenceincalcium ion levels in hypothyroidismwomen compared with those non- hypothyroidism women (control group).
302 MOLECULAR DETECTION OF BACTERIAL VAGINOSIS AND ITS ASSOCIATION WITH MISCARRIAGE IN AL-HILLAH CITY, Ilham A Bunyan, Asmaa K Gates, Alaa K Hameed
This study attempt to detect some BV associated bacteria and their associations with miscarriage in vaginosis women. One hundred fifty (150) high vaginal swabs were collected from married vaginosis women, from the hospital of Babylon city and private clinics.Seventy five samples were taken from vaginosis women without miscarriage and Seventy five samples from vaginosis women with miscarriage.Bacterial vaginosis (BV) women was diagnosis as having BV according to Amsel's criteria. The age of patient (15– 45) years. The sample was collected by disposable swabs.This swab stored in freezing until were used for molecular analysis, DNA was extracted from these swabs and the 16s rRNA genes of some bacterial vaginosis bacteria detection by polymerase chain reaction technique. Molecular result showed that G. vaginalis 139(92.66%) and Atopobium vaginae 116(77.33%) were more common than other causes of bacterial vaginosis. This result showed that the highest rate of Vaginosis occurred in low education (Illiterate) level 85 (57%) and most cases of miscarriage occurred at the first trimester 45(60%).Alsothe role of the infectious agent with miscarriage investigated, The results indicated astatistically significant difference between multiple miscarriage and single miscarriageofin women that infection with Megasphaerasp, Atopobiumvaginae, G. vaginalis and Leptotrichia sp (p=0.050%, p==0.022%,0.038%0.036% respectively).
303 TEMPORARY EMBARRASSMENT AND THE RELATIONSHIP BETWEEN THE PERFORMANCES OF SOME BASIC SKILLS WITH THE VOLLEYBALL TEAM PLAYERS IN THE PROVINCE OF DIYALA, Ali Hameed Ali, Saddam M Ahmed, Sardar HM Amen
The study aimed to found the relationship between the critical time and some basic skills in volleyball. The study tackled the concept of critical stress and its importance in the mathematical aspect. It also discussed some basic skills related to the momentary embarrassment from the point of view of the trainer). Fifteen players of the Diyala Youth Education Team were used in present study. The player was chosen in a deliberate manner due to the cooperation of the coach and the players with the researcher. The candidate's tests were used and presented to the specialized experts. After using the appropriate statistical means, the study was show important conclusions such as significant correlation relationship between the regret and some basic skills of volleyball players of Diyala Youth Education. The most important recommendations are the need for psychological attention to the players and the appointment of a psychologist specialized in sports psychology to accompany the team during the competition as well as training the players to provide psychological skills to cope with the stressful events during the game and to emphasize the importance of critical time in training in teams and sports clubs in the country especially the care of psychological and informative sides of the players. Work to provide the psychological environment for the players and that contributes to the creation of opportunities for creativity and thinking of them, and conduct periodic tests to assess the psychological pressures and embarrassing situations and ways to address them.
304 HISTOPATHOLOGICAL FINDINGS IN ENDOMETRIAL BIOPSIES FOR A SAMPLE OF IRAQI WOMEN WITH ABNORMAL UTERINE BLEEDING, Noora M Kareem, Enas T Mousa, Sara A Al-Rawaf
Abnormal uterine bleeding is a major gynecological problem in our community, This cross sectional study aimed to review the histopathological findings in endometrial biopsies of 288 patients from different age groups who have been admitted to Al- Imamain Al-kadhimain medical city in Baghdad complained of abnormal uterine bleedingResult:The percentages of functional causes were as follows: proliferative endometrium 24.7%, secretory 9.4%, weakly proliferative 0.3%, menstrual endometrium 1%, atrophic endometrium 3.5%,decidual reaction 8.7%. the percentages of the organic causes were:abortion 29.9%, molar pregnancy 5.9%, endometrial polyp 5.9%,endocervical polyp 2.4%, endometritis 1%, simple (non atypical) endometrial hyperplasia5.6%, atypical endometrial hyperplasia 1.4% and one case (0.3%) was diagnosed as endometrial carcinoma
305 A STATISTICAL STUDY ON THE NATURE AND TYPE OF RADIATION POLLUTION AND ITS EFFECT ON THE ENVIRONMENT AND HUMAN, Eman Ibrahim Awad, Fadhel Y Khudheyer, Ohood Salman Jawad
Our current study focuses on the definition of radiation pollution and the study of its main causes, sources, main types and its direct and indirect impact on human life. Industrial waste is the main source of radiation emission in nature, and it is this radiation that directly affects the environment in which humans and other living organisms live. In addition to study the effects of this radioactive contamination in the world, and how to control it and eliminate threats to the environment. This is because these industrial wastes from different industries are therefore transformed into radioactive nuclear waste that harms humans and living organisms and cause two kinds of pollution: internal radioactive pollution and external radioactive contamination. On this basis, industrial waste can be considered a nuclear energy that can be used to generate energy that must be used by humans in an orderly manner rather than being wasted indiscriminately and not benefiting from it
306 Clostridium botulinum, A FOODBORNE PATHOGEN AND ITS IMPACT ON PUBLIC HEALTH, Manal HG Kanaan, Ahmed M Tarek
Clostridium botulinum (C. botulinum) is an important cause of human disease, especially in hospitalized patients, as well as in animals. Botulism is a debilitating sickness that disturbs the body's nervous system and is initiated by incorporation of powerful neurotoxins produced by C. botulinum bacteria. Botulism typically associated with the ingestion of botulinum toxin (BoNT), which is already involved in food, and therefore, toxins arrive the human body through the gastrointestinal tract to produce the standard form of botulism are called consumption botulism. The outbreak of the food industry contaminated by BoNT is a public health crisis. The bioterrorism threat regarding the deliberate distribution of BoNT food and / or the aerosolization of BoNT raises public health and safety due to high mortality and potential morbidity. The aim of this review was, therefore to open up the idea of the disease by C. botulinum and its impact on public health to protect consumers from diseases and toxins transmitted by food. Meeting these criteria, the issue of C. botulinum with the net programs of modern foods satisfies food poisoning, reducing the transmission of these pathogens and their spores and / or toxins from food and feed. There are many unanswered questions about the epidemiology of this pathogen, and it would be prudent to monitor the ongoing research on this organism to determine whether it represents a risk as a foodborne pathogen.
307 MICROWAVE SYNTHESIS OF SOME NEW ORGANO SELENIUM COMPLEX COMPOUNDS AS (LIGANDS), DERIVATIVES FROM SCHIFF BASES AND PROVE THEIR STRUCTURES BY USING FTIIR SPECTRUM, Abed M Daher Al-libory, Sarham A Salman, Essa M Thalig
The microwave technique is a new method for synthesis of organic compounds which results in pure and high percent of products and completed reactions with short time, compared with conventional method (heating method). The prepared Schiff bases allowed to react with (Selenium Metal, Se34, 1st serial of transition elements), to yield Selenium complex compounds as (Ligands). The useful thing in microwave technique is using without solvent there for it called (dry method). The synthesized compounds were identified using melting point apparatus, (FT.I.R) Infrared spectroscopy.
308 Molecular study of multiple myeloma, Sarah F Buniya, Mohammed A Jebor
Multiple myelomas malignant proliferation of monoclonal plasma cells in the bone marrow can be characterized by skeletal destruction, renal failure, anemia, and hypercalcemia. The blood samples were collected from 70 patients who admitted Merjan Teaching Hospital in Babylon Province, Iraq, from February to July 2018. In addition to 70 healthy persons who had been considered as a control group. Genotyping of multiple myeloma genes was performed by utilizing a polymerase chain reaction (PCR) technique, followed by single-strand conformation polymorphism (SSCP) and RFLP. We found that the molecular detection of three type of multiple myeloma mutations in genes: TP53 exon 8-9 using (RFLP) technique had been showed that 1 band 13(18.57%) and 2 band 57(81.42%) in patients while gene TP53exon 7 4 band 50(71.24%) and 5 band 20(28.6%) in patients and FGFR3 using (SSCP) the results show that 1 band 13(18.57%) and 2 bands 55(78.5%) in patients. To conclude, we suggest that the variants of Multiple myelomas SNP.
309 PLANTS- DERIVED MATERIALS AND THEIR EFFECTS AS ANTICANCER AGENTS: A REVIEW, Dhifa J Shamran, Emaduldeen H Abed
Cancer is the main cause of death worldwide, in spite of improvement in early detection and treatments, and this request an active strategy for prevention and curing. Plants represent a huge source for bioactive materials that well known in folk medicines from thousands of years. The plants and plants derived compounds safe, low-cost, available and the toxic side effects are lower than the common used treatments, for this reason, considered as target for researches from decades. Several plants derived compounds have been identified as anticancer agents and considered as promising candidates for anticancer therapy. There, we focus on some these compounds and its mechanisms of action.
310 Evaluation of bacterial profile with serum CRP, RF and anti-CCP antibodies in acute and chronic tonsillitis patients in Babylon province , Bareq A Al-lateef, Najla'a Jwad Hasani, Entesar Marzoog Hussain
This study compares the diagnostic of ”Tonsillitis" is inflammation of the pharyngeal tonsils and is also known as pharyngitis to extend the inflammation to the adenoid and the lingual tonsils. Most cases of bacterial tonsillitis occur group β-"hemolytic Streptococcus pyogenes" (GABHS), "S. aureus" generally. The current study aims to bacterial profile assessment with serum CRP, antibodies RF, and Anti-CCP in acute & chronic tonsillitis patients in Babylon Province. Completely, (45) a sample of blood and swabs of (22-56) years of male and female tonsillitis patients. Sera antibodies of (CRP, RF, and anti-CCP) identified by serological techniques for every one of these patients. Results showed that the culture of the samples gave all patient 45 positive growths and the presentation of the positive conclusion in grams of positive bacteria and negative grams depending on the staining. The highest percentages of S. pyogenes and S. aureus in patients with tonsillitis of other pathogens. Additionally, it is the highest proportion of tonsillitis disease is unlike the healthy individual's group according to different criteria by age group (21-59) years, disease duration (4-13) years, females and autoantibody tests (CRP, RF, and Anti-CCP antibodies) in patients with tonsillitis patients (acute & chronic). Our study concluded that it is important to determine the values of these criteria for their effects on patients suffering from acute and chronic tonsillitis.
311 Effect of genetic polymorphism MHFR C677T in some biochemical markers in a group of βT patients, Wagdi S Sadeq, Zubaida A Kudaier, Ali N Sabeeh, Faik I Ali
The current study was conducted as an attempt to know about the prevalence of the mutant genotype TT, caused by substitution mutation C – T, in MTHFR C677T and the effect of resulted genetic polymorphism in the levels of hyc in Iraqi patients of βT registered in Kirkuk public hospital, Kirkuk- Iraq, in addition to a try to reveal if any change have been happened in biochemical parameters in the group of patients compared to healthy control group. The number of patients is 50, 25 males and 25 females, the mean of age and standard deviation 8.32 ± 4.41, and 45 of the healthy control group, 20 males and 25 females, with age, mean and standard deviation 9.51 ± 3.41. The percentage of related parents is 38% in the group of patients, while it is 51.1% in the control group. Data of four of patients and seven of healthy individuals were excluded due to the failing of getting amplified output of blood DNA. Electrophoresis of PCR products after incubation with restriction enzyme Hinfl, showed 198bp band which belong to the normal allele C, and two bands of 175bp and 23bp which belong to mutant allele T. Calculation of allele frequency showed increase in the mutant allele in the group of patients which reached 0.368 compared with 0.046 in healthy control group. On the other hand, the results showed a higher frequency of homozygous genotype of mutant allele TT in the group of patients 10.86% compared to 2.32% in the healthy control group. The test of Chi2 showed a significant difference in numbers of observed and expected genotypes, calculated Chi2 value 0.66 compared to tabulated value 5.99 with 2 degrees of freedom and 0.05 level of significance, while it reached 9.84 for the control group. The results of the biochemical study showed no significant differences in levels of hyc between patients and healthy individuals. The value of mean difference and standard error mean reached (2.33 ± 1.56, t= 1.49), the levels of folate reached (1.31 ± 1.05, t= 1.24. While there were significant differences in the levels of ferritin (-387.18 ± 16.79, t= 23.05) and VB12 (202.99 ± 14.49, t= 14.0).
312 STUDY OF SOME BIOLOGICAL ASPECTS OF MICE INFECTED WITH HYDATID CYST OF Echinococcus granulosus PARASITE AND THE THERAPEUTIC CAPACITY OF ETHANOLIC EXTRACT OF Cladophora glomerate ALGAE, Hadeel A Majeed, Amal K Abbas, Shatha K Abbas
This study aimed to find the effect of Cladophoraglomerata extract on some Biological, aspects of hydatid cyst of Echinococcus granulosus in mice infected with cystic Echinococcosis and evaluating the efficiency of algae extracts in treating the disease. Forty mice were used, and were divided to 4 equal groups; three groups of mice were injected into the peritoneal cavity with protoscolices, while the fourth group was left as a negative control group. After 4 months of infection the second and third groups were given the extraction of Cladophoraglomerateat concentration 128mg/ml and 256mg/ml respectively, while the first group considered control positive. Six months after the start of the infection, mice were dissected, the organs examined. The levels of some liver enzymes, the concentration of some metals and some immunological parameter were measured, the study found that the algae extract in two concentrations decrease the activity of ALP (p≤0.05) in treated group, a significant increase was observed in the concentrations of Ca and Na in the treated groups (p≤0.05), The algae extract also had a significant reduction in the level of IL2 and IL4(p≤0.05), It can be concluded that the ethanol extract of Cladophoraglomerata contains effective substances that can be used to treat the disease caused by Echinococcus granulosus
313 Assessment the correlations of albumin creatinine ratio ACR and thyroid Hormones in patients with thyroid disorders and renal cell carcinoma, Areej GH Al-Charak
The incidence of thyroid disease has increased worldwide and the patients with thyroid disorders are also predisposed to other types of disorders including renal cell carcinoma. Human RCC is thought to arise from a variety of specialized cells located along the length of the nephron. We aimed to assess the correlations of albumin creatinine ratio ACR and thyroid hormones in patients with combined of hyper or hypothyroidism and renal cell carcinoma to the investigation of which study groups (hyper or hypo) have a risk to the incidence of RCC. This case-control study included four groups, the first group included 20 patients with hyperthyroidism, the second group of 20 patients with hypothyroidism, the third group were 20 patients with RCC, and 20 healthy subjects are as a control group. We investigation of TSH, fT3, fT4, Albuminuria, Creatinine, ACR in all groups. We found that the serum level of TSH was significantly lower in patients with hypothyroidism (HPOTH) group (1.23 ± 0.43 μIU/ml) compared to hyperthyroidism (HPRTH) (13.32± 3.2 μIU/ml), RCC (10.12± 2.7 μIU/ml and control group (4.12 ± 1.3 μIU/ml), (p-value
314 Assessment the correlations of albumin creatinine ratio ACR and thyroid Hormones in patients with thyroid disorders and renal cell carcinoma, Areej GH Al-Charak
The incidence of thyroid disease has increased worldwide and the patients with thyroid disorders are also predisposed to other types of disorders including renal cell carcinoma. Human RCC is thought to arise from a variety of specialized cells located along the length of the nephron. We aimed to assess the correlations of albumin creatinine ratio ACR and thyroid hormones in patients with combined of hyper or hypothyroidism and renal cell carcinoma to the investigation of which study groups (hyper or hypo) have a risk to the incidence of RCC. This case-control study included four groups, the first group included 20 patients with hyperthyroidism, the second group of 20 patients with hypothyroidism, the third group were 20 patients with RCC, and 20 healthy subjects are as a control group. We investigation of TSH, fT3, fT4, Albuminuria, Creatinine, ACR in all groups. We found that the serum level of TSH was significantly lower in patients with hypothyroidism (HPOTH) group (1.23 ± 0.43 μIU/ml) compared to hyperthyroidism (HPRTH) (13.32± 3.2 μIU/ml), RCC (10.12± 2.7 μIU/ml and control group (4.12 ± 1.3 μIU/ml), (p-value
315 Effect of Salix alba barks on experimentally induced obesity in rats , Aymen A. Bash, Abdul Kareem H. Abd
Obesity is a multifactorial cradle that grows from the interaction of metabolic, cellular, molecular, psychological, behavioral and social factors. This condition characterized by increasing in content and size of adipose tissue, and fat accumulation, leading to an increment in the weight of the body. The objective of the present study is to investigate the effects of Salix alba barks as herbal medicine on body weight parameters, lipid profiles, and metabolic inflammation in rats' experimental models of obesity. In the current study, we used forty apparently healthy Wister male rats were involved in the present study, divided into four groups. Obesity was induced by the administration of high-fat diets for 8 weeks to stimulate adiposity. The first group received a normal diet with a daily dose of distilled water intraperitoneally and left without treatment and considered as a control group. The remaining three groups that were induced to be obese, received (Orlistat 35 mg/kg and Salix alba 150mg/kg) after the induction period for 28 days, except one group left without treatment. The blood was collected from all animal groups, and then serum obtained for the biochemical analysis. Our results showed that in obese animals, body weight and body mass index were significantly reduced by Salix alba after 4 weeks of treatment. Fasting cholesterol, TG, LDL, VLDL, TNFα, and visfatin and leptin were significantly decreased, while HDL, IL10, and adiponectin significantly elevated by Salix alba compared with HFD induced obese animal group. To conclude, Salix alba barks improve lipid profile as well as reducing the elevated body weight. Salix alba barks inhibit metabolic inflammatory response associated with obesity. The adipose tissue-derived adipokines were got better with this herb.
316 VARIATION IN PD-1/PD-L1 PROTEIN EXPRESSION IN LIVER AND SPLEEN AFTER TREATMENT OF CYSTIC ECHINOCOCCOSIS: AN EXPERIMENTAL STUDY, Talib Waseen Hussein, Waheeda Rashid Ali, Haider F. Ghazi
The stimulatory signaling molecules programmed death-1 and its ligand (PD-L1) is associated with immunoregulatory and suppressive immune effector mechanisms. The aim of this study is to evaluate the liver and spleen tissue expression of PD-1 and PD-L1after treatment with Oxfendazole (OXF), Oxfendazole + Praziquantel (OXF+PZQ), Oxfendazole +Albendazole (OXF+ABZ) and Albendazole +Praziquantel (ABZ+PZQ) of experimentally mice model of cystic echinococcosis. After mice model induction of hydatid disease, four months of treatment were initiated and both liver and spleen were processed for immunohistochemical staining of PD-1 and PD-L1proteins.The results showed that higher expression of PD-1 and PD-L1proteins in positive control group and statistically reduced expression in treated groups suggesting an involvement of PD-1/PD-L1signaling pathway in the pathogenesis of cystic echinococcosis
317 ISOLATION AND MOLECULAR DETECTION OF DERMATOPHYTES FROM HORSES IN THE GOVERNORATE OF WASIT, IRAQ, Isa S Touhali, Mohammed I Mohammed
Dermatophytosis causes superficial fungal infection that poses public health problems to man and animals and economically significant causes of numerous dermatomes' that have been observed in equine species, therefore the aim of this study to investigate the distribution of dermatophytes species in horses and comparing the traditional methods with PCR techniques for detection of dermatophytes in the governorate of Wasit,Iraq. A total of 50 horses samples were collected from skin scrapings suggestive of dermatophytosis from diverse ages, sexes and areas, during the period of June to August 2018. The results showed that 32 (64%) out of 50 horses sampled were positive for dermatophytes by direct microscopic, while cultures only 26 (52%) out of 50 horses sampled were positive results for dermatophytes and results shown all culture positive samples were also positive in direct microscopic. In this study which exposed most frequent dermatophytes isolates were Trichophyton and Microsporum species in cultures and five species were recovered, T. equinium was the most common species 9 ( 34.6%) out of 26 positive culture, followed by T. mentagrophytes7(26.9%), M. equinium 5(19.3%), T. verrucosum 3(11.5%), M. gypseum 2(7.7%)and 6 isolated of non-dermatophytes were also detected. This study showed all culture positive samples were also positive in PCR technique. While six isolates were positive by direct microscopic show negative result by PCR technique. This indicates the infected of the skin with other types of non-dermatophytes. In conclusion that Trichophyton and Microsporum species were most frequently occurring dermatophytes in horses and PCR targeting the (Chs1) gene may be considered confirmed correct identification and screening test for detection of dermatophytes in horses.
318 ISOLATION OF SOME BACTERIAL SPECIES ASSOCIATED WITH CANDIDIASIS AND STUDY OF THE BIOLOGICAL ACTIVITY OF Alliumsativium AQUEOUS EXTRACT AGAINST THEM, Alaa Y Rahy, Alaa M Al-Rubae, Ali K Sarboot
Total of 80 samples were collected from infants suffering from candidiasis infection present in Al-Refai general hospital from February 2019 to October 2019. The samples were cultured using malt extract Agar for the growth of Candida spp and using MacConkey agar and blood agar to isolates gram negative and positive bacteria. Yeast were diagnosed according to some normal test used in diagnosis such as phenotypic traits, Chlamydia spores and bacterial isolates were diagnosed according to some biochemical tests garlic was obtained from local market, then the aqueous extract of garlic was prepared in different concentrations as: 50,100,150,200,250 g/ml. The results showed that there are four bacterial species: Escherichia coli, Staphylococcus aureus, Klebsiella spp and Streptococcus pneumonia. S. aureus was the highest (50%) and the lowest was Klebsiella spp. (2.5%). The results showed that the use of garlic aqueous extract at the concentration 250g/ml has the highest antibacterial activity and the concentration 50g/ml has the lower antibacterial activity. The study isolates some bacterial species associated with candidiasis and concluded that garlic aqueous extract have an effect against these bacterial species so; this study recommended using this plant as antibacterial natural source.
319 ANALYSIS OF WOMEN WITH ENDOMETRIAL HYPERPLASIA MEASURED BY U/S IN THE CONTEXT OF SOCIO-DEMOGRAPHIC AND OTHER RISK FACTORS IN LAST FIVE YEARS IN AL-HILLA CITY IN IRAQ, Ban Aamer Mousa, Sijal Fadhil Farhood Makki Al Joborae,Hassan Falah AL-Khafaji
Objective:-Assessment of presence of risk factors of this disease, year’s through highly associated prevalence of the endometrial uterine hyperplasia from 2014-2018 & age-related prevalence of endometrial hyperplasia, in perimenaupausal patients with abnormal vaginal bleeding.
320 THE EFFECT OF THE TRAINING CURRICULUM ON DEVELOPING KINETIC AND TRANSITIONAL RESPONSE SPEED, ACCURACY OF THE STOPPING WALL PERFORMANCE, AND DEFENDING THE STADIUM IN VOLLEYBALL, Mohamed D Mohamed, Rafid H Mohammed, Tarik D Mohamed
The research aims to equip a training curriculum to develop the motor response speed, transition speed, and accuracy of the performance of the skills of the wall defense of the stadium in volleyball,in addition to identifying the effect of the proposed training approach in developing the variables under research. The researchers have assumed that there are statistically significant differences between the results of tribal and dimensional tests in The development of kinetic response speed, transition speed, and accuracy of the stopping wall defense and defending field skills in favor of distance tests, as well as having statistically significant differences between the results of dimensional tests for the two research groups, developing the variables under investigation. The researchers used the experimental method using the two groups. Then, the research community (Balad Club) was identified as a volleyball for the 2019/2020 sports season. In light of these results, the researchers reached the following conclusions: - There were statistically significant differences between the results of the pre and post tests in developing the motor response speed and the transition speed and the accuracy of the performance of the blocking wall skills and defending the field in volleyball and in favor of the dimensional tests. - There were statistically significant differences between the results of the two dimensional tests of the two research groups in developing the kinetic response speed and the transitional speed and the accuracy of the skills of the blocking wall and defending the field in volleyball.
321 DESCRIPTIVE STUDY OF CUTANEOUS LEISHMANIASIS IN WASIT PROVINCE /IRAQ IN 2018, Azhar Jabbar Al Subaihawi, Taqi Mohammed Jwad Taher, Firas Turki Rashed Sarray, Ahmed Emad Ajeel
In relative to other skin diseases, cutaneous leishmaniasis (CL), consider a mild disease which well known as oriental sore. Cutaneous Leishmania species transmitted in a specific species of sandflies, as biological vectors that spread the disease to humans. This study was done to describe epidemiological aspects of cutaneous leishmaniasis in Wasit province in Iraq, including thedescriptive epidemiological distribution according to person, place and time in 2018. This cross-sectional study was including all the urban and rural regions of Wasit province located in eastern Iraq.Data on human cases were prepared by Wasit health office for the year2018. Data were analyzed by SPSS version 23 using frequency distribution. Patients with cutaneous leishmaniasis in Wasit province characterized by that (74.6%) of them were less than 10 years old, with the lowest percentage in old age patients (>70) years old.Regarding gender, there is no apparent difference between males (50.9%) and females (49.1%).The disease was more prevalentin patients living in rural (62.5%) areas than in urban areas (37.5).The highest percentage of registered cases was in the Kut primary healthcare district during November and January. Cutaneous leishmaniasis in Wasit during 2018 affecting younger age groups mostly of both sexes living in rural areas.Most of the cases registered in winter monthsespecially in the AL- Kut health district.
322 ASSOCIATION OF PROGRAMMED CELL DEATH PROTEIN–1 ( PD-1 ) GENE POLYMORPHISM AND SERUM LEVELS OF SOLUBLE PD-1 WITH TYPE 2 DIABETES MELLITUS, Aya Raed Rasheed, Wafaa Ragee, Qasim S AL-Mayah
Type 2 diabetes mellitus (T2DM) is a metabolic disorder of multiple etiologies characterized by chronic hyperglycemia with disturbances of fat and protein metabolism resulting from defects in insulin secretion, insulin action or both, and mediated in large part by the alteration in adaptive immunity. This study aimed to evaluate the role of programmed cell death protein – 1 (PD-1) gene polymorphism and serum level of soluble PD-1 in development of T2DM in Iraqi patients. Forty-five T2DM patients were recruited for this study. Other 45 apparently healthy subjects matched for age, gender and ethnic background for patients were also included as control group. Blood samples were collected from each participant, and DNA was extracted from leukocytes. The gene fragment corresponding the PD-1-538 G/A polymorphism was amplified with conventional PCR using specific primers. The genotyping was performed through restriction fragment length gene polymorphism (RFLP). Serum level of soluble PD-1 (sPD-1) was measured by enzyme linked immune sorbent assay (ELISA). There was no significant association between different genotypes of PD-1-538 G/A polymorphism. However, at allelic level, G allele was less frequent among patients than controls (28.89% versus 44.44%) with a significant difference. The median concentration of sPD-1 in patients was 53.12 pg/ml (range 18.24-312.89 pg/ml) compared to 63.83pg//ml (range 16.89-508.65 pg/ml) in controls with no significant difference. Median levels of sPD-1 in patients carrying GG, GA and AA genotypes were 51.67 pg/ml (range 18.24-160.41 pg/mL), 52.77 pg/ml (range 19.32-154.18 pg/mL) and 220.97 pg/ml (range 129.05-312.89 pg/mL) respectively. The GG genotype carriers differed significantly from AA genotype carriers, while there was no significant between AA genotype carriers and AG genotype carriers. These data suggest that A allele of PD-1-538 G/A might be a risk factor for development of T2DM. The GG genotype of this polymorphism associates with higher serum level of sPD-1 than other genotypes.
323 LEVEL OF PHYSICAL MOTOR INTELLIGENCE AND ITS RELATIONSHIP TO THE ACCURATE PERFORMANCE BLOWS PLAYING ON THE NET BY BADMINTON, Ziad M Farhan, Marwan A Yousef, Mohammed A Awad
The study aims to identify the level of physical motor intelligence in the study sample, also to identify the relationship between the level of physical motor intelligenceand the accuracy of strokes performance on the net which is (blow hair pin, fatal blow, lightning strike). The sample was represented by University of Anbar badminton team. Statistic case (SPSS) was used to acquire results were the researchers conclude that the sample has an unfit physical motor intelligence level, in which physical motor intelligence is very important in learning skills in which the higher the physical motor intelligence the faster the learning process was.
324 OXIDATIVE STRESS, ANTI-MELANOCYTE AND ANTI TYROSINASE ANTIBODY IN VITILIGO AND RESPONSE TO TREATMENT, Ahmed Danbous Obayes Al-Hussainy, Moaed E. Al-Gazally, Wesam Ewadh
Vitiligo is an acquired, usually asymptomatic pigmentary disorder that results in the loss of functional melanocytes and is often associated with other autoimmune diseases. At the onset of the disease white patches of different sizes appear on different parts of the body. Vitiligo usually affects the skin, but it can develop anywhere we have pigment. Patches of hair can turn white. Some people lose color inside their mouths. The affected skin can lighten or turn completely white. Vitiligo affects approximately 1% of the world population of all skin types, usually before the age of 20. In this case-control study (100) patients with vitiligo which divided in to two subgroups (treated with topical, systemic steroid and treated with narrow band phototherapy), and (50) healthy control were examined. VASI score for each patient was calculated. Serum levels of GPx activity &TAC were measured by spectrophotometer,but MC-Ab & TYR-Ab aweremeasured by ELIZA. After measurements and comparison between vitiligo patients and ageand-gender matched control group, results showed significant differences in glutathione peroxidase activity, total antioxidant capacity, anti-melanocyte antibody and anti tyrosinase antibody among these groups. There was significant increase in serum GPx activity, TAC, MC-Ab and TYR-Ab (P value = < 0.001, 0.003, < 0.001 and < 0.001 for glutathione peroxidase activity, total antioxidant capacity, anti-melanocyte antibody and anti tyrosinase antibody respectively) for vitiligo patients as compared with control group. After measurements and comparison between vitiligo patients subgroups results showed significant increase in serum GPxactivity among NB-UVB group, but no significant differences in TAC, MC-Ab and TYR-Ab were showed when comparison between vitiligo patients subgroups. There was significant increase in serum GPx activity (P value =
325 EFFECTIVENESS OF NURSING EDUCATIONAL PROGRAM ON NURSES’ KNOWLEDGE REGARDING PAIN MANAGEMENT FOR POSTOPERATIVE PATIENTS, Mohammad K. Abdul-Jaleel, AH Rajha
Pain is one of the universal medical problems in health institutions. In fact it can directly affect patients' comfort and lifestyle, causing nausea, vomiting, fatigue, stress, lack of control, poor quality of life, and a lack of sexual activity and public relations, also it can lead to poor work performance and daily activities. The aim of the study is to investigate the effect of nursing educational program about postoperative pain management for postoperative patients. A quasi- experimental design has been applied on (50) nurse to evaluate the effectiveness of an educational program on nurse’s knowledge about management of patients with postoperative pain in AL-Najaf AL-Ashraf city at Al-Sadder medical city through the period October 1st, 2018 to, August 18st, 2019. The results of the study show there is a high significant effect of the educational program regarding nurses’ knowledge toward postoperative pain management. The study concluded that the majority of nurses in surgical ward had knowledge deficit concerning management of patient with postoperative pain. The study recommends to encouraging nurses to enroll in sessions of special courses and conference to improve their knowledge and keep them up to date toward postoperative pain management.
326 IDENTIFICATION AND PHYLOGENETIC STUDY OF Klebsiella pneumoniae VIA MOLECULAR-BASED TECHNIQUES TARGETING 16S rRNA, magA, and rmpA IN CAMELS FROM AL-DIWANIYAH CITY, IRAQ, Basima Jasim Mohammed, Zeena Foaad Saleh, Jameela Radi Esmaeel, Saba Falah Klaif, Mohammad Sabri Jawad, Khulood Hamdan Fahad
This study was as an investigation to identify the presence of Klebsiella pneumoniae in the livers and lungs of camels in Al-diwaniyah City, Iraq. Here, 50 specimens were sampled from each organ of 50 camels. In the beginning, regular cultivation and biochemical tests were performed for primary detection. Then,a multiplex polymerase chain reaction (mPCR) targeting specific pieces of 16S rRNA, mucoviscosity-associated A (magA), and regulator of mucoid phenotype A (rmpA) genes was done for more confirmative identification of the bacterium. After that, the PCR-based products of the 16S rRNA gene were subjected to partial sequencing in which the results were used to generate a phylogenetic tree to compare our isolates with some previously identified strains of the K. pneumoniae. The results of the primary detection showed the presence of this bacterium in 30 (60%) and 42 (84%) of the liver and lung samples respectively. The mPCR results revealed the amplification of the tested genes at 30(100%)16S rRNA,25 (83%) magA, and 0 (0%) rmpA in the liver samples and 42(100%) 16S rRNA, 35 (92%) magA,and 30 (78.9%) rmpA in the samples of lungs. The sequencing results identified 2 isolates (MH119051.1) and (MH119052.1) which gave close matching with some global stains such asKM203760.1, an Indian isolate. Our study gives valued information about the presence of this bacterium that causes infection to the camels in the studied city.
327 COMPARISON BETWEEN PCOS AND NON-PCOS WOMEN REGARDING FOLLICULAR FLUID IL-10,IL-12, IL-17A AND IL-23 LEVELS, Zuhud Mawlood Mustafa,Rafal Abdullah Saleh, Huda Hussam Abulhussein
This study was conducted on the patients attended special private clinics for the treatment of infertility in Baghdad city for the period from the end of July 2015 until the end of September 2016 for estimation of interleukin (IL)-10, IL-12, IL-17A and IL-23 in in the follicular fluid of “polycystic ovary syndrome (PCOS) women.The study included 55 women with polycystic ovary syndrome and 35healthy women. The follicular fluid was collected from the patients and the control group during the ovulation period, i.e. 12-14 days of the menstrual cycle, 3 ml of follicular fluid were collected by physicianand then kept in the refrigerator freezing until for estimation oflevel of interleukin -10, IL-12, IL-17A and IL-23 in in the follicular fluid of “PCOS women” by using ELISA technique (Quantikine Co. USA).The current study showed that most PCOS, who reached the age of twenty to twenty-nine years and 60%of them andexcess weight. All polycystic ovarian women were all affected by the appearance of hair in the bodies here (hirsutism). Study also showed that 65.45% ofPCOScases were suffering from the appearance of acne in their skin and majority of cases were with irregular menstrual cycle and positive family history of the syndrome and 74.55% were with alopecia”.“The study demonstrated thatIL-10 was significantly elevated in PCOS women” (P. value 0.0004) “as compared with healthy women” (33.8±3.71 vs. 17.5±2.84pg/ml)”.“The study demonstrated thatIL-12 was significantly reduced in PCOS women” (P. value 0.0012) “as compared with healthy women” (1.26±0.02 vs. 3.09±0.011 pg/ml)”.“The study demonstrated thatIL-17A was significantly elevated in PCOS women” (P. value 0.001) “as compared with healthy women” (22.8±4.78 vs. 16.9±3.19 pg/ml)”.“The study demonstrated thatIL-23 was significantly decreased in follicular fluid of PCOS women” (P. value 0.008) “as compared with healthy women” (7.19±1.71 vs. 13.71±1.92 pg/ml). It was concluded that IL-10and IL17A were elevated in PCOS women while IL-12 and IL-23 were reduced as compared with control group.
328 VESICOVAGINAL FISTULA REPAIR WITH AND WITHOUT INTERPOSED FLAP; IS THERE ANY DIFFERENCE?, Yassir Ismael Abbass , Mazin Anwar Yadgar , Azzawi M Hadi
The abnormal communication between bladder and vagina (Vesicovaginal fistula VVF) is a devastating problem leading to continuous leakage of urine to the vaginal vault and resulting in significant morbidity for the affected female patients. Various techniques have been described for the management of VVF, but with significant recurrence rate of vesicovaginal fistulae. In our study we tried to evaluate the outcome of VVF repair with the use of omental flap interposition, and comparing it with VVF repair without flap. A prospective comparative trial conducted from July 2014 to May 2019, to evaluate the end result of trans-abdominal VVF repair (with or without interpositional flap) on 33 patients in Tikrit, mostly 3 to 6 months after their diagnosis. Excluding those patientwith a complicated fistula and malignant fistula. The selected patients were randomized into two groups. Group A (18 patients) was repaired with using omental flap, whereas, group B (15 patients) was repaired without omental flap interposition. A total of 33 patients with VVF were included in our study. With amean age of 36.6 years (range 23-43). The majority of VVF (66.5%) occurred as complications of abdominal hysterectomy, and obstructed labor is the second most common cause (account for about 31.5% of the cases). With fistula size ranging from 16 Fr to 30 Fr calibrated using Foly's catheter and finger calibration in larger fistula. The total reported success rate of the surgical repair in this study was 94%.In 18 patients were omentum flap interposition used; all of them except one (94.4%) were cured, while in 15 patients, repair done without flap, 13 (86.6%) patients were dry in the post-operative period. Though omentum flap interposition have good result in preventing recurrence of vesicovaginal fistulae, fistula repair without flap can give a high success, if meticulous dissection and suturing is done.
329 PROCALCITONIN AND C-REACTIVE PROTEIN AS PROGNOSTIC BIOMARKER OF INFECTION IN TRAUMATIC FRACTURES, Ali Adulrazzaq Mohammed, Shaimaa Essa Ahmed, Mutaz Sabah Ahmeid
The study was conducted in AL-Fallujha teaching hospital for the period from Januaryto December 2018 on 100 patients with different fractures, (50 of them were without the presence of infections after the repair of the fracture and the other 50 were suffering from inflammation of the surgery after the repair). All information such as age, weight and sex of all patients in the study included the measurement of white blood cell level, reactive protein C level, procalcitonin level (i-chroma/immunofluorescence) and comparison of the two groups in relation to these variables as well as comparison in regard to time after the operation. In this work, total white blood cells count was significantly higher in fractured patients with infection than fractured patients without infection.In this study, CRP level was significantly higher in fractured patients with infection (10.81±1.2 mg/ml) than fractured patients without infection (8.33±0.93 mg/ml). In this study, procalcitonin (PCT) level was significantly higher in fractured patients with infection (163.3±5.9 pg/ml) than fractured patients without infection (131.7±4.8 pg/ml).The study revealed positive correlation of PCT with WBCs and CRP levels in infected fractured patientsand no correlation of PCT with WBCs and CRP in non-infected patients. The study revealed positive correlation of PCT, WBCs and CRP levels with duration after surgical operation in infectedtrauma fractures patients below 3 days and weak correlation of duration above 4 days while the study showed no correlation of PCT, WBCs and CRP levels with duration after surgical operation of non-infected trauma fractures patients. Procalcitonin and CRP were significantly related with infected patients after surgical fracture operation and could be used as markers for determination of infection after surgery.
330 Using real time PCR technique to detect antioxidant PRDX1 gene for proteins in mitochondria for autistic patients, Nahid Ahkzarmadhloom Turky, Assist. Prof Zubaidah Adnan Khudhair Aljashaami
In the current study, the level of gene expression of one of the genes in the genetic material of mitochondria had been measured. The gene is one of six genes belonging to the family of peroxiredoxin enzymes, PRDX1. This family of enzymes is considered responsible for the production of antioxidant enzymes. Our study had been included 35 sick children (age 2- 14 years), who were compared to 14 healthy children (control group). The children were from various cities including Baghdad - Kirkuk - Salah al-Din. The level of gene expression was measured by extraction of RNA from the hair follicles of the patients and their mothers as well as for control group. We used the Real Time-PCR technique and statistical analyzes, including extraction value of Fold expression by 2 - (∆∆Ct), as well as the applying of T-test and F-value of the samples.The results showed a significant difference in the level of PRDX1 gene expression in autistic children compared with healthy children. the results showed that the level of expression of the PRDX1 gene in autistic children compared with healthy children and a significant difference of -2.32 for the T test and a level of probability (P≤0.05), and was similar This value was decreased with the expression of this gene in the sick children compared to their mothers with a significant difference of 2.15 for the T test and a probability level of (P≤0.05), while no significant difference was observed between the mothers of patients and healthy mothers. In general, the F-value test for the four groups showed a significant difference in the gene expression of the PRDX1 gene by 2.46 with a probability level of (P≤0.05).
331 Relation of common ABL kinase domain mutations with resistance to Tyrosine Kinase Inhibiters in patients with Chronic Myeloid Leukemia in Middle Euphrates of Iraq, Muhammed Sadiq Mahdi Al-Musawi, Sameer Hasan Abbood, Liwaa Hussein Mhadi, Rahem Mahdy Rahem
Chronic myeloid leukemia (CML) is a hematopoietic stem cell disease, associated with a reciprocal translocation between chromosomes 9 and chromosome 22, lead to the formation of the BCR-ABL fusion gene (Philadelphia chromosome). This fusion gene is believed to play golden role in the initial development of CML with constitutive tyrosine kinase activation. Successful use of tyrosine kinase inhibiters (TKIs) play a role in improve survival and increase prevalence of CML, but un fortunately mutations in the BCR-ABL kinase domain may cause, or contribute to increase, resistance to TKIs in CML patients. This study was designed to assess the association of seven most common BCR-ABL kinase domain mutations (T315I, M351T, E255K, G250E, Y253F, M244V and E255V) with resistance state of CML patients on TKIs in Iraqi Middle Euphrates region, a retrospective case-control study in which 85 patients with chronic myeloid leukemia in chronic phase (45 patients as cases group and 40 patient as control group) were selected from three hematooncology centers in middle Euphrates in Iraq during the period from January 2016 till October 2016 out of a total of 240 CML patients (108 male and 132 female) who were registered during this period in these three center sand all patients on TKI (Imatinib and Nicotinic), venous blood sampling done for BCR-ABL kinase domain mutations screening. T315I, G250E and E255V among seven ABL kinas domain mutations were detected in our CML patients with resistance to TKIs. All of them may play a role in development variable degree of resistance to first and second generation TKIs weather primary or secondary.T315I mutation is most common mutation within BCR-ABL domain kinase gene.
332 Relation of common ABL kinase domain mutations with resistance to Tyrosine Kinase Inhibiters in patients with Chronic Myeloid Leukemia in Middle Euphrates of Iraq, Muhammed Sadiq Mahdi Al-Musawi, Sameer Hasan Abbood, Liwaa Hussein Mhadi, Rahem Mahdy Rahem
Chronic myeloid leukemia (CML) is a hematopoietic stem cell disease, associated with a reciprocal translocation between chromosomes 9 and chromosome 22, lead to the formation of the BCR-ABL fusion gene (Philadelphia chromosome). This fusion gene is believed to play golden role in the initial development of CML with constitutive tyrosine kinase activation. Successful use of tyrosine kinase inhibiters (TKIs) play a role in improve survival and increase prevalence of CML, but un fortunately mutations in the BCR-ABL kinase domain may cause, or contribute to increase, resistance to TKIs in CML patients. This study was designed to assess the association of seven most common BCR-ABL kinase domain mutations (T315I, M351T, E255K, G250E, Y253F, M244V and E255V) with resistance state of CML patients on TKIs in Iraqi Middle Euphrates region, a retrospective case-control study in which 85 patients with chronic myeloid leukemia in chronic phase (45 patients as cases group and 40 patient as control group) were selected from three hematooncology centers in middle Euphrates in Iraq during the period from January 2016 till October 2016 out of a total of 240 CML patients (108 male and 132 female) who were registered during this period in these three center sand all patients on TKI (Imatinib and Nicotinic), venous blood sampling done for BCR-ABL kinase domain mutations screening. T315I, G250E and E255V among seven ABL kinas domain mutations were detected in our CML patients with resistance to TKIs. All of them may play a role in development variable degree of resistance to first and second generation TKIs weather primary or secondary.T315I mutation is most common mutation within BCR-ABL domain kinase gene.
333 Comparative study between anti-ccp and rheumatoid factor as diagnostic value of rheumatoid arthritis patients, Rawaa Behlul Alfatlawi, Huda Ghazi Al-Masshadi, Wasan Sami Hameed, Taghreed Dheyaa Aljazaeri
Objectives Rheumatoid arthritis disease is an autoimmune infirmity disease. For avoiding the damage of joint and organ, early diagnosis and early treatment are the superior means. This study aimed to compare between ACCP (anti-cyclic citrullinated peptide antibody) and rheumatoid factor (RF) as the diagnostic value of rheumatoid arthritis patients (RA). Method This study carried out on RA patients, who clinically diagnostic, were admitted at the physical medicine and rehabilitation clinics of Al-Sader Teaching Hospital between December 2017 and February 2018 prospectively. The protocol of the current study was approved by the medical ethics of Ministry of Health and Environment according to the American Rheumatism Association. By using ELISA kit Anti-CCP and RF were determined. A total of 207 serum samples was collected from RA patients, which consist of 86 males and 121 females. At the same time, a total of 93 serum samples was collected from non-RA persons (control), which consist of 42 males and 51 females. SPSS (version 17.0) was used for statistical analysis; T-test was used to test whether there is a different between non-RA and RA groups. Also, RA correlation with Anti-chip was tested. Results The results showed that there was a positive correlation between values of ACCP Log10 and RF Log10 of male and female patients (P-value
334 ECG markers of sudden cardiac death among body mass index groups, Yasi Salah ALam, Karar Nadhm Obaid Aljabry, Hussein Nafakhi, Abdulameer A Al0Mosawi, Hasan A AL-Nafakh
Body mass index (BMI) is frequently used anthropometric measure to assess the relative weight in large cardiovascular studies. However, the relationship of obesity measured by BMI with ECG markers of repolarization-depolarization abnormalities is controversial in the literature. The paper aims to assess the distribution of novel ECG markers iCEB (QT/QRS), TDR (Tp-e interval and Tp-e/QT) among body mass index groups (normal weight, overweight and obese) in patients with normal coronary arteries compared to patients with coronary atherosclerosis. The patients and methods are 117 patients (age: 55±10 years, 49% males) who underwent 64-slice multi-detector CT angiography examination to exclude coronary artery disease were found to be eligible and were enrolled in the study. The results are the higher Tp-e values were observed among normal weight and overweight patients compared to obese patients (P= 0.002) while there were no significant differences in QRS, QT, QT/QRS and Tp-e/QT observed between BMI groups in patients without coronary atherosclerosis. In patients with coronary atherosclerosis, there were no significant differences in the distribution of ECG markers between BMI groups (P
335 Radiological feature of chest x-ray in chronic obstructive pulmonary disease correlation with clinical finding, Shameem Ali Hussein, Alaa Yas Abd, Mohammed Thejil Abdallah
Chronic obstructive pulmonary disease (COPD) is an important health problem as it is associated with significant morbidity and mortality, it is now the fourth leading cause of death globally .The long-acting bronchodilators have proved to be the most effective therapies so far available and Since COPD patients are at increased risk for co-morbidities, in these last years it has been realized that there is a strong need to look beyond the lungs in treating patients with COPD. Regarding the chest X -ray finding to exclude alternative diagnosis, evaluative of comorbidities e.g. interstitial lung disease ,lung cancer with air ways obstruction ,in acute exacerbation look for complicating processes e.g. pneumonia ,cardiac failure ,pneumothorax . This study which was carried out on 25 case sheets of patient with COPD taken from the statistic departments of AL- Hussain teaching Hospital in order to clarify the role of x -ray regarding their ( finding in COPD) also the effect of smoking and the presence of associated diseases on radiological appearance , analyzed by excel and as a result it has been found that smokers had more incidence to having COPD than nonsmokers also patients who had associated diseases had longer duration of hospital stay and comorbidity.
336 Antibacterial and Cytotoxic Effects of Silver Nanoparticles on Staphylococcus aurous and Normal Vero Cells, Rasha Hadi Saleh, Entisar J Al-Mukhtar, Zaytoon A Alkhafaji, Mohammed H Al Hasnawy, Huda H Al-Hasnawy
Silver (nanoparticles) (AgNPs) are of special concern as a result of their unique chemical, physical and biological characteristics. It has become an attractive alternative to antibiotics due to their broad-spectrum antimicrobial activity. The study aimed to determine the antibacterial activity of AgNPs against S. aurous bacteria and the effect of AgNPs on the-viability of normal cell line (Vero cell). A1total of 70 clinical 1samples (-wound and vagina swab, stool and urine) were used in this study. Bacterial isolates were subjected to the microscopically, cultural and biochemical evaluation, AgNPs were-prepared and checked for their antimicrobial activity by the use of 1various concentrations employing agar dilution method. In addition, the effect of different concentrations of AgNPs on a viability of Vero cells was examined. The results showed that out of 70 clinical samples, 11 (15.7%) isolates were Staphylococcus aurous. AgNPs showed high activity against S. aurous at concentrations (100 μg/ml and 200μg/ml). It was found that there was no effect of AgNPs on the viability of the normal Vero cells at (≤ 250 μg/ml) concentration, but they have cytotoxic effect on the viability of the these cells at high concentrations. This study concluded that AgNPs possess good antimicrobial-activity and the concentrations that maintain the cell viability could be used as an alternative therapy to treat S. aurous infections.
337 Mutational analysis of osteoporosis of pregnancy – associated osteoporosis, Ali Taha Hassan Al-Azzawi
Pregnancy associated osteoporosis (PAO) is very rare condition with an estimated incidence of (4-8 in million pregnancies), it is idiopathic. The aim of this study is to evaluate the genetic study of (PAO) and mutational analysis to detect the cases which are susceptible to develop osteoporosis and make a good strategy of treatment for them to relive the symptoms and prevent the occurrence of osteoporosis in the subsequent pregnancies. In this study we examined sixty pregnant women in feminine and pediatric teaching hospital in Samawa city at the period from January, 2018 to August, 2019. All the patients were sent for measuring the level of serum vitamin D3 which showed most of the patients had decreased level of vitamin D3. All the patients sent for ultrasound of the foot to evaluate the osteoporosis which showed all of the patients had osteoporosis. All the patients sent for genetic study and mutational analysis including: 1) DNA Extraction, 2) Gel Electrophoresis, 3) Polymerase chain Reaction (PCR), and 4) DNA Sequencing to evaluate single Nucleotide polymorphism (SNP).The study revealed fourteen pregnant women with mutation genes which are susceptible to develop osteoporosis and subsequent pregnancies and to treat them and prevent occurrence of the disease in the future pregnancies.
338 Possible Protective Effect of Zucchini Juice against Nephrotoxicity Induced by Cisplatin in Mice, Entisar Jawad Al-Mukhtar, Asmaa M Mekkey, Zehraa Manji Saleem, Huda H Al-Hasnamy, Nisreen Al-Bayati, AHmad Rashid Ibrahim, Zaytoon A Alkafaji
Cisplatin (CP) is a widely used anti-cancer drug that is commonly administered for the treatment of various cancers. However, nephrotoxicity is the most important adverse effect of this drug which limits its use. Aim of the present study was inspection of the possible protective effect of zucchini juice against CP-induced nephrotoxicity. In this experimental study mice were equally divided into three groups: the positive control group (CP only treated group), control group (distilled water only treated group) and zucchini juice group (CP plus zucchini juice treated group), then the biochemical (blood urea and serum creatinine) and histological examination were done. Results revealed that the renal indices (blood urea & serum creatinine) in the positive control group were increased high significantly (p ≤ 0.001) in comparison to the control group and zucchini juice received group, while no significant difference was found between control group & zucchini juice treated group (p ≥ 0.05), also histopathological assessment of kidney's sections taken from mice of the positive control group revealed that there were diffuse renal tubular necrosis and infiltration of inflammatory cells as compared to the normal renal tubules that were appeared in the kidney's sections taken from mice of the control group. While no histopathological changes were seen during the examination of histopathological sections of kidney's mice of the zucchini juice group as compared to the histopathological sections of the kidneys taken from mice belong to the positive control group and control group. In conclusion zucchini seems to have a protective effect against nephrotoxicity induced by CP.
339 Comparative study between anti-ccp and rheumatoid factor as diagnostic value of rheumatoid arthritis patients, Rawaa Behlul Alfatlawi, Huda Ghazi Al-Mashhadi, Wasan Sami Hameed, Taghreed Dyeyaa Aljazaeri
Objectives Rheumatoid arthritis disease is an autoimmune infirmity disease. For avoiding the damage of joint and organ, early diagnosis and early treatment are the superior means. This study aimed to compare between ACCP (anti-cyclic citrullinated peptide antibody) and rheumatoid factor (RF) as the diagnostic value of rheumatoid arthritis patients (RA). Method This study carried out on RA patients, who clinically diagnostic, were admitted at the physical medicine and rehabilitation clinics of Al-Sader Teaching Hospital between December 2017 and February 2018 prospectively. The protocol of the current study was approved by the medical ethics of Ministry of Health and Environment according to the American Rheumatism Association. By using ELISA kit Anti-CCP and RF were determined. A total of 207 serum samples was collected from RA patients, which consist of 86 males and 121 females. At the same time, a total of 93 serum samples was collected from non-RA persons (control), which consist of 42 males and 51 females. SPSS (version 17.0) was used for statistical analysis; T-test was used to test whether there is a different between non-RA and RA groups. Also, RA correlation with Anti-chip was tested. Results The results showed that there was a positive correlation between values of ACCP Log10 and RF Log10 of male and female patients (P-value
340 Assessment of Low Electric Current Intensities, Exposure Time and Electrodes Distance Effects on Bacterial Quantity in un-Chlorination Water, Ibraheem Abdulrahman Eltaif, Sundus Jassim Muhammad, Dunia Kamal Salim
In recent years, there has been a renewed interest in the use of electric field to inactivation of bacteria in water, air, wound, muscle, biofilm and others. Although the electrical methods have been widely studied, the specific factors as different of electric current intensities, distance of electrodes, exposure time and river water as medium, that influence in bacterial number, remained largely unclear. Therefore this study aimed to investigate the use of low electric field to reduce the bacterial quantity in raw water. The following factors: different low electric current (LEC) intensities (50, 100 and 200mA) at fixed voltage (220v-DC), distance between electrodes (25, 50 and 75cm) and exposure time (30and 60min) were applied, whereas water temperature did not reach 40 ºC at the end of the treatment. Many random samples of un-chlorination of river water (raw) were submitted in this study.Our results showed that the application of the three different LEC intensities on bacteria in river water inside cylindrical plastic basins (25, 50 and 75 cm) showed significant influences due to the kill or inhibition of bacterial cells. The largest Ratio of Reducing Bacterial Quantity (RRBQ) was 96.25 % at 200mA for 60min when the distance of electrodes was 25cm.In conclusions; This study rise the understanding of LEC process effects on the bacterial concentration in un-chlorinated river water.
341 Study the relationship between various bacterial infections and lipid profile in Samarra hospital patients, Osama N Nijris, Faesal G Hassen, Asmaa E Mahmood
This study included diagnosis 125 sample of patients from various diseases,The infections were divided into 25 Samples of patients with each of Urinary Tract Infection (UTI), Pharyngitis, Typhoid fever,Brucellosis and Gastric ulcer infection,we used classical procedure in diagnosis for UTI and Pharyngitis sample, but the last three infections were diagnosed by serological methods only, The results showed that only 80% of UTI and70% of throat infection (Pharyngitis) sample show bacterial growth, The kinds of bacterial in UTI were Staphylococcus aureus 50, Escherichia coli 25, Klebsiella pneumonia 15%, Pseudomonas aeruginosa 10%, Whereas The kind of bacteria in Pharyngitis was Streptococcus pyogenes only for all samples, whereas the positive results for Typhoid fever, Brucellosis and Gastric ulcer infection were 85%, 40%, 68% respectively. We hold analyzes lipid profile on serum samples to see how the effect of these infections on Total Cholesterol (TC), Triglycerides (TG), High Density Lipoprotein (HDL),Low Density Lipoprotein(LDL) and Very Low Density Lipoproteinstandards value (VLDL), We were found that there variation in the results according to the type of bacteria and type of infection, have shown that the infectionsdecreasing levels of TC,HDL and LDL and slightly elevation of TG standards in UTI, Pharyngitis and Typhoid fever while in Helicobacter pyloriinfection results has shown decreasing in levels of HDL and slightly elevation in TC,TG and LDL, Whereas in Brucellosis results has shownslightly elevationin standards of TC and LDL.
342 Screening, purification of tannase produced from Iraqi Klebsiella pneumonia isolates and its role in enhancement of biofilm inhibition formed by Enterobacteriaceae isolates, Ahlam Khaleefah Abdal, Sawsan Sajid Al-Jubori, Sahira Nsayef Muslim
In this study, tannase enzyme was produced from an Iraq Klebsiella pneumoniae isolates. K. pneumoniae were identified by Vitek system and confirmed by housekeeping 16s rRNA gene (amplified size 155 bp). Tannase was genotpically detected by amplification tanA gene (amplified size 210 bp) followed by sequencing. The tannase activity reached its maximum level when this isolate was cultivated under the optimal conditions which are consisted of using 2.8 g of nutrient agar containing 2% (w/v) tannic acid as a carbon source at pH 5.5 and temperature of 37°C for 24 h. The Tannase had been purified by using three methods: ammonium sulphate, ion exchange and gel filtration. The first method leads to gain a tannase precipitation at 70% ammonium sulphate which is considered as a partial purification where tannase activity was 80U/ml. In comparison, 300 U/mg tannase activities had been gained by using ion exchange with 4.31 fold of purification and a yield of 21.4%. Finally, a tannase activity of 500 U/mg is gained by using gel filtration with 5.75 fold of purification and a yield of 21.4%. The purified tannase is a single peptide with approximate molecular mass of 46.5 kDa as assessed by SDS-PAGE. The purified tannase have been inhibited biofilm formation ability in a dose-independent manner by all gram negative Enterobacteriaceae.
343 Effect of different doses of sublingual misoprostol on pain experience during office hysteroscopy, Nadia Mudher S Al-Hilli
Office hysteroscopy is considered nowadays as the gold standard investigation in patients with perimenopausal or postmenopausal bleeding, irregular bleeding & infertility patients. Its use as an outpatient procedure erase the need to make it least uncomfortable by preparing the patient using drugs that soften the cervix & allow to introduce the hysteroscope easily. The aim of this study was to evaluate different doses of sublingual misoprostol on pain score & its side effect on the procedure. This prospective study involved 88 women planned for office hysteroscopy were selected randomly and divided into 3 groups, 25 patients received 200 µg misoprostol sublingually one hour before the procedure, 30 patients received 100 µg the same way as the first group and the 3rd group 33 patients did not received misoprostol, after the procedure patients were asked about their pain using pain analogue score pictogram with a score from zero to ten. Results of this study revealed that the main indication of hysteroscopy in this study was with irregular vaginal bleeding 48.9% followed by infertility 22.7%. Main finding was endometrial polyp 59.5%. Pain score was significantly higher in patients who did not receive misoprostol 4.09 compared with those who received (2.36 & 2.13 for the 200 µg & 100 µg groups respectively). No statistical difference was found between the two doses of misoprostol regarding pain score. However, side effects in form of endometrial congestion & poor distention of uterine cavity was more in the 200 µg misoprostol group compared to 100 µg misoprostol group (56% versus13.3% and 8% versus 0% respectively). Conclusion: use of misoprostol 100 µg sublingually one hour before office hysteroscopy decrease pain experienced by patient & minimally affecting the endometrium & visual field.
344 Fractionation and Nominal Standard Dose in Radiotherapy, Rawah Naji Al-Shammary
The Nominal Standard Dose (NSD) concept is frequently utilized in patients treated with radiation, as it involves the relationship of overall time with total dose and fractionation (TDF) factors. Careful patient planning of patients involves giving them a full dose determined by physiotherapists in the form of batches rather than once, for the purpose of minimizing the biological effect of radiation. Since the divided dose is given daily during the week, the number of fractions should be defined as the treatments the patient takes each week. In this paper we intend to identify and analyze many aspects of radiobiology, and how the NSD can be shown from the tables.
345 Association of Coat plumage Colors (CPC) for Iraqi local chicken and some productive performance trait by using some analysis parameters , Eman Hassan Al-Anbari
To study relations between different coat plumage colors (CPC) in both sexes for Iraqi local chicken with some performance productive traits as mature body weight and weights at 2, 3, 4 periods /4 weeks and for egg product, egg weight, egg mass and regression predication equation for mature body weight on body weight at 2, 3 and 4 periods and for mature body weight on egg product, egg weight and egg mass, to be used in early selection programs, One hundred mixed-sexes of local Iraqi chicken at three months age old were distributed in five groups according to coat plumage color as CPC G for gray, CPC B for brown, CPC R for red, CPC W for white and CPC BL for black coat. Result indicated that CPC BL group was differ significantly (P≤0.05) in mature body weight and on WT2, WT3 and WT4 period with means of 910.83gm, 1028.67gm, 1146gm and 1249.94gm CPC W and CPC R color groups was with 1121.89gm and 1121.17gm at the same level of signification. Males was differ significantly on all studied periods for means weight than females. Means for egg productive traits show no significant difference between different groups, and for regression equation it was highly significant (P≤0.01) at all body weight period and results indicating that the regression of matured body weight effect and caused increasing body weight at all periods, and the coefficient of determination R2 of mature body weight with body weight at WT2, WT3 and WT4 are with value of 0.88, 0.73, and 0.63. Plumage chicken group for CPC G coat color show highly significant effect with (P≤0.01) probability for maturing body weight on all of egg product, egg mass and egg weight with R2 of 0.004, 0.008 and 0.08 for the coefficient of determination. The obtained results showed possibility gives evidence that depending on coat plumage color for local chicken with black and white coat color for the relation on body weight, to select chicks for meat purposes rather than for egg production, and for males than females and for local chicken with gray plumage coat for egg product traits and that can be improved by using modern traditional selection depending and beside on the modern molecular technique to serve Iraqi local domestic chicken as national identity for Iraqi.
346 The effect of complex skill exercises to develop the kinematic speed of rolling and Suppressions football for young adults (14-16 years), Muhammed Mussa Lazim Al-Essawi
The development in the level of football was not a coincidence but rather the efforts of specialists and experts in the field of the game to multiply the basic skills and their diversity in football, making the technical numbers of these skills take more attention and complex exercises is one of the most important exercises of the game that works to develop the skill side of the player Football and the speed of the kinetic response is considered one of the capabilities that are not free from any sporting activity. Naturally, the need for it varied from one activity to another according to the skill and planning requirements in each activity and the research problem was that there is a slow movement speed when performing scoring and handling skills and research goal setting exercises special skill to develop a vehicle-speed motor rolling and suppressions junior aged (14-16 years). The research hypothesis there were statistically significant differences between the pre and posttests in the experimental and control groups and theoretical studies concerned with complex exercises, kinetic speed and basic skills in football and rolling the ball and Suppressions and the research sample was (16) players from the Amo Baba School of Football for young people ages (14) -16 years. The search tests were the choice of kinematic speed, the choice of rolling and the choice of Suppressions, and the researcher discussed the results, and from the research conclusions the effect of complex exercises for the ball in developing the kinematic speed of rolling and Suppressions for members of the experimental group, hence the importance of the research in preparing special exercises to develop kinematic speed. Rolling and Suppressions football.
347 Histopathological picture of heat cured acrylic resin modified with nano-composite filler (in vivo study), Aseel Abdullameer Radhi, Ali Fadhil Hashim, Thanaa Ghani
Heat cured polymethyl methacrylate is a biomaterial with a cytotoxic effect modified by composite fillers to improve its mechanical properties need to evaluate tissue reaction to the modified materials. Material & Method A 45 heat cured acrylic disc specimens were divided into control group which is acrylic resin specimens without reinforcement, and test group specimens reinforced with the silanatedAlumium silicate nanoparticle powder fillers in a concentration ratio is 3% and 5%. These specimens were implanted in a 15 Wister rats. The implanted areas undergo biopsy after 3days, 14 days, and 4 weeks. Then, prepared for histopathological examination. Results Within the limitation of this study the results revealed that the control groups induce more tissue reaction and show a significant inflammatory cell count as compared to modified test groups in all observation periods Conclusion Modification of heat cured acrylic denture base resin with alumino silicate nano particles in concentrations 3% and 5% consider morebiocompatible than unmodified groups.
348 Histopathological and Molecular Studies For An association Between Breast Cancer and Epstein-Barr virus In Iraqi Population, Abeer Cheaid Yousif Al-Fatlawi,Al-Nasrawi Adil Ateyah A. A,Haider Jebur Kehiosh
The majority type of cancer between women in worldwide is breast cancer (BC). The relationship between Epstein-Barr virus (EBV) and breast carcinogenesis is still controversial. The goal of the present research was to explain the involvement of EBV and its role in development of BC in both females and males in Karbala population. In the present study, sixty two subjects were divided into four groups as the following: (group I: 32 women infected with BC, group II: 10 intact women as a control, group III: 10 men infected with BCand group VI: 10 intact men as a control) and evaluated using paraffin embedded tissues. In this survey study, higher percentage (46.875%) of affected females with BC were found at age (41-50) years and (25%) of affected males with BC were found at age (51-60) years. DNA of EBV in pathological sampleswas detectedby Polymerase chain reaction (PCR) as well as PR and ER markers was analyzedvia immunohistochemistry techniques, inthe result, there was no significant difference between the grade of EBV tumor with the age groups between females and males. Furthermore, there were no significant differences at (P> 0.05) in females in which 20/29 (69%) of females have been affected with IDC with positive EBV-DNA in their investigated samples, while 9/29 (31%) of females were negative, OR (1.11) IC (0.088-13.89). Moreover, 2 /3(66.7%) of females have been affected with ILC with positive EBV-DNA in their investigated samples, while1 /3 (33.3%)of females werenegative. On the other hand, 8 / 10 (80%) of the Male patients were affected with IDC and they were positive for EBV-DNA in their investigated samples, while 2 /10 (20%) of males were negative, and there was no significant differences in ages between affected patients.
349 The majority type of cancer between women in worldwide is breast cancer (BC). The relationship between Epstein-Barr virus (EBV) and breast carcinogenesis is still controversial. The goal of the present research was to explain the involvement of EBV and its role in development of BC in both females and males in Karbala population. In the present study, sixty two subjects were divided into four groups as the following: (group I: 32 women infected with BC, group II: 10 intact women as a control, group III: 10 men infected with BCand group VI: 10 intact men as a control) and evaluated using paraffin embedded tissues. In this survey study, higher percentage (46.875%) of affected females with BC were found at age (41-50) years and (25%) of affected males with BC were found at age (51-60) years. DNA of EBV in pathological sampleswas detectedby Polymerase chain reaction (PCR) as well as PR and ER markers was analyzedvia immunohistochemistry techniques, inthe result, there was no significant difference between the grade of EBV tumor with the age groups between females and males. Furthermore, there were no significant differences at (P> 0.05) in females in which 20/29 (69%) of females have been affected with IDC with positive EBV-DNA in their investigated samples, while 9/29 (31%) of females were negative, OR (1.11) IC (0.088-13.89). Moreover, 2 /3(66.7%) of females have been affected with ILC with positive EBV-DNA in their investigated samples, while1 /3 (33.3%)of females werenegative. On the other hand, 8 / 10 (80%) of the Male patients were affected with IDC and they were positive for EBV-DNA in their investigated samples, while 2 /10 (20%) of males were negative, and there was no significant differences in ages between affected patients., Makarem M. Abdulkareem
Aims: To evaluate the disinfection potential of different exposure times of Ultraviolet Light (UV-light) on upper removable complete dentures. Materials and Methods: A total of 100 patients with upper complete dentures were selected randomly to be involved in this study. All of the tissue sides of the involved dentures were exposed to four shots with 5 minutes to each. Swap sampling of each exposure to the UV-light (5- 20) minutes was collected and cultured. Colony forming unit (CFU) for each of isolated species was determined based on grown of bacterial colonies. Results: A total of 34 strains of bacteria distributed between 12 strains of Gram-negative and 22 strains of Gram-positive were diagnosed. The sensitivity of these bacteria to various shots of UV-light was variable between Gram-negative and Gram-positive. Colony counts of some isolated was noted to decrease along with increase the exposure time of UV-light. Two isolates of Gram-negative and 6 isolates of Gram-positive were shown more sensitive to UV-light as indicated by the absence of growth after first shot of light (5minutes). Some types of bacteria exhibited resistance to the first two shots of UV-light (10minutes) and their growth started to decline after the third shot (15minutes). Conclusions: This study showed that removable dentures could be disinfected with UV-light.
350 Relationship of adjustment, social competence and achievement motivation among senior secondary school students, Nimisha Beri, Manjinder Kaur
Adjustment is a satisfactory and tuneful relationship of an organism to its environment. Social competence refers to those skills necessary for effective interpersonal functioning. Achievement motivation is based on attainment success and achieving all our aspiration in life. In order to obtain relevant and accurate data descriptive survey method and stratified sampling was used in the present study. 478 senior secondary school students were taken as sample for study. The result of the study reveals that senior secondary school students have lowest level of achievement motivation, average level of social competence, very unsatisfactory level of home adjustment, health adjustment, social adjustment and emotional adjustment. Statistically significant difference was found in Adjustment and Social Competence and Achievement Motivation with respect to gender, type of schools and locality. The achievement motivation of senior secondary school students of government schools was higher than that of private schools. Senior secondary school students of urban area showed higher level of social competence then the students of rural area. Adjustment was not found correlated to social competence and achievement motivation very little influence of social competence and achievement motivation was found on adjustment.
351 Corporate Social Responsibility for climate change using social contracts: A new research agenda, Sanil S Hishan, Suresh Ramakrishnan, Ahmad Jusoh
Climate change is one of the long persisting problems in the past few decades. Political leaders, world organizations, corporate sector and the citizens are affected by the climate change. The catastrophic effect of climate change is the increasing frequency and magnitude of disasters across the globe. Hence, this is a global problem which needs a global solution. The main aim of this paper is to propose a model for disaster mitigation which can be sustainable. The study uses a systematic literature review approach which includes the articles published in different databases. The output proposed a new conceptual model using a social contract theory where there is a holistic approach and each one takes up the responsibility to mitigate disaster between all the stakeholders. The study aligns with one of the seventeen Sustainable Development Goals (SGD) set by the United Nations (UN).
352 Validation of developed instruments on study habits, creativity, and self concept on college students in Nigeria, Sulaiman Adamu Mayanchi, Aqeel Khan, Adibah Binti Abdul Latif
The role of validating a questionnaire is to ensure that the questionnaires for use in research settings are psychometrically sound, valid and reliable as well as efficient and effective. This article will therefore provide the validation of existing resources on study habits, creativity and self-concept through the use of the Rasch Measurement approach. A pilot study was randomly selected from each of the two Colleges in Zamfara State, with a sample of 180 respondents. The employed Rasch-based Winsteps code produces the required parameter estimate automatically. The results of the reliability coefficients showed the reliability of 0.83, 0.96 and 0.87 items, with 0.96, 0.91 and 0.94 corresponding to Cronbach Alpha. The unidimensional construct of the test measures supported by the raw variance explained by measurements of 48.3 percent, 53.8 percent and 53.0 percent closely match the variance expected. Investigation of the map of the item person revealed that all items fell within the respondents ' ability level. Likewise, fitness indices showed that 5 items were listed to delete items for study habits, and sub-scale creativity reveals that all 23 items in the scale have reasonable fitness while 5 items have poor self-concept sub-scale fitness indices. The results confirm the accuracy of the explanations and inferences of the scores on the objects and subscales of the instruments.
353 Culture, socio-economic status, and religious coping as predictors of happiness: a review, Zainab Ibrahim Lawal, Aqeel Khan, Jamaluddin bin Ramli
Happiness importance cannot be overemphasized this has been confirmed by many researches and world happiness report 2012—2018. As a result, factors predicting happiness have been employed by researchers and scholars in various studies on Muslim women. Thus, this study examined and found out the factors predicting happiness such as culture, socio-economic status (SES) and religious coping as well as identified the foremost and majorpredictor and research design used by these scholars. A systematic review of the literature on culture, socio-economic status and religious coping as predictors of happiness among Muslim women was carried out. A total of number of 30 articles from different disciplines has been examined. The factors and research design found were divided in to those that belong to happiness predicted by culture, by socio-economic status, by religious coping and by culture, socio-economic status and religious coping, happiness related to culture, happiness related to culture, to socio-economic status, to religious coping and related to culture, socio-economic status and religious coping. The research designs were quantitative, qualitative, mixed method and systematic literature review (SLR). Quantitative research design was found to be the leading research design.The studies were also examined based on countries of study; Iran, Turkey, Palestine, Oman, Qatar, New Zealand, England, Bangladesh, Algeria, Jordan, Indonesia, Malaysia and Lebanon. Iran was the leading country. But no model of happiness was found in the studies analysed. It is hoped that findings from this study will be beneficial to Muslims in women centre for continuing education and counsellors especially in sub-Sahara Africa (Nigeria).
354 Homoeopathic perspective of environmental factors in stone diseases: a mini review , AS Suman Snakar, Aminyananda Dev Goswami, NV Sugathan, ST Gopukumar
Homeopathy is based on the claim that illnesses can be treated using substances that produce similar symptoms. Mostly, these have been heavily diluted in water or alcohol so that none or only a few molecules of the active ingredient are present. Kidney stone disease is a crystal concretion formed usually within the kidneys. It is an increasing urological disorder of human health, affecting about 12% of the world population. It has been associated with an increased risk of end-stage renal failure. The etiology of kidney stone is multifactorial. The most common type of kidney stone is calcium oxalate formed at Randall's plaque on the renal papillary surfaces. The mechanism of stone formation is a complex process which results from several physicochemical events including supersaturation, nucleation, growth, aggregation, and retention of urinary stone constituents within tubular cells. These steps are modulated by an imbalance between factors that promote or inhibit urinary crystallization. It is also noted that cellular injury promotes retention of particles on renal papillary surfaces. This article is an endeavor to explain its adequacy dependent on later methodical surveys on kidney stones. Electronic databases were looked for orderly audits/investigation regarding the matter. Forty-one articles satisfied the consideration/rejection criteria. It is concluded that the best environmental clinical evidence for homeopathic medicines available up to date and permit positive endorsements for its use in clinical practice.
355 Effect of calcarea carbonica on osteoporotic activities in ovariectomised albino rat model of osteoporosis in 1x, 30, and 0/1 potencies , Winston Vargheese, Alphy Mathew, CK Mohan, Krishna Kumar Amma
Objective: To study the effect of Calcarea Carb, in osteoporotic activity, in ovariectomized rat model of osteoporosis with 3 different potency scales -1X, 30 and 0/1, to compare the effect of Calcarea Carb with estrogen in controlling osteoporosis and to study the potency that is more suitable in treating osteoporosis, in ovariectomized rat model of osteoporosis. Materials and Methods: Healthy female Wistar albino rats were divided into 5 groups of 6 animals each. Ovariectomy was done for all the groups. Group 1(control) was fed with saline. Group 2 was orally treated with estrogen (2mg/kg) and Groups 3-5 were orally treated with Calcarea Carb in potencies 1X, 30 and 0/1 respectively, for 90 days. After 90 days the rats were sacrificed for bone physical parameters, uterine weight, body weight, estimation of serum levels of calcium, phosphorous, alkaline phosphatase and osteocalcin and also for histopathological estimation. Result: For all parameters, except body weight and histopathological examination, Calcarea Carb 1X showed higher result. For the histopathological estimation of femur and body weight, Calcarea Carb 0/1 showed the highest result. Conclusion: Calcarea Carb is effective in controlling osteoporosis in all the potencies, particularly in 0/1 potency.
356 Morbid Obesity and Laparotomy Myomectomy–A Case Report, Cipta Pramana, Belinda Sentosa, Geofanny F Chandra, Zamila Khairatunnisa
Morbid Obesity and Laparotomy Myomectomy–A Case Report
357 The effect of expressive writing technique to stress level decrease of new student at Al-Falah Putri Islamic Boarding School, Margodadi, Tanggamus, Hamid Mukhlis, Ani Kristianingsih, Fera Fitrianti, Teguh Pribadi, Desi Kumalasari, Hellen Febriyanti, Andino Maseleno
Stress related to new learning environment is considered as a serious problem in the boarding education. Research showed the benefits of expressive writing on stress for new student in Islamic boarding school. Based on the Expressive Wrinting technique we designed Expressive writing training to reduce stress level and introduce mental health to new student. This study used quasi-experimental method with the design of one group pretest-posttest design. The training was held for five days with the number of respondents was 14 new students who had no experience in Islamic boarding schools before and still attending regular school in class VII MTS. Data was measured twice: the pretest and posttest used DASS-42 Stress Questionnaire. Bivariate analysis in this study used the Paired t Test. The results showed that all participants of the research program showed a decrease in stress after being given Expressive Wrinting intervention in new student. There was difference in stress level between pretest and posttest at 6,214 points. Expressive Writing therapy should be used as a treatment program that can be done to reduce stress on new students.
358 Homoeopathy for dysgraphia in school children, NV Sugathan, CK Mohan, Amiyananda Dev Goswami, V Suju
Introduction: Dysgraphia is a specific transcription disability which results in illegible handwriting, inconsistent spacing, poor spatial planning on paper, poor spelling, difficulty in composing writing and thinking at the same time. There are 3 types of dysgraphia based on which manifestations alter - spatial, motor, or dyslexic. Mostly these 3 types are associated. Dysgraphia can be treated and managed successfully, especially if the disability is identified in early childhood or adulthood. As dysgraphia affects the person completely and impaired his/her quality of life, Homoeopathy is found to be the appropriate choice of treatment as it considers its patients as a whole. Methods: 30 school children who were diagnosed with Dysgraphia were randomly selected for this study. Medicines will be selected based on individuality of the child. Conclusion: From this study it is found that Homoeopathy is effective in managing Dysgraphia. The efficacy of Homoeopathy medicine on Dysgraphia was assessed by comparing before and after treatment writing scores with LDDI. In this study it is observed that majority of patients are males who belongs to the age group of 8 to 10 years and Dysgraphia is associated with various co-morbidities like ADHD, Anxiety disorder, ODD and CD.
359 Three dimensional structure modeling and ramachandran plot analysis of autographa californica nucleopolyhdro viral protein, ST Gopukumar, Sreeya G Nair, R Radha, NV Sugathan, Anooj ES, Lekshmi Gangadhar
Background: Autographa Californica’s nucleopolyhedro is a viral protein found in baciloviridae. It is made of double stranded DNA genome. They viral protein easily infects this insect and affects the expression system. Method: The main aim of this work is to predict various viral proteins (Autographa Californica nucleopolyhedro). FASTA sequences are retrived from NCBI (National Center for Biotechnology Information) database. Then Basic Local Alignment Search Tool (BLAST) used to find regions of similarity in different sequences. The homology modeling was done in swiss model. The template model also constructed. Results: Comparative modeling of this program to predict the protein sequence identity is 46.83%.The template protein e value is 110 and they chain A having identify 100% similarity maximum score 320.Further the protein structure was validated in Ramachandran plot. Conclusion: From Ramachandran plot analysis it was found that the portions of residues falling into the favored regions was 98%.The predicted 3D model further characterized and analyzed using other techniques.
360 In vitro anti-arthritic activity of cnidoscolus aconitifolius latex extract by protein denaturation method, S.Agneswari,Sreeya G. Nair, R. Radha, C.K. Mohan, ST Gopukumar
The study is intended to gauge the Latex of Cnidoscolus aconitifolius for commended anti-arthritic activity using in vitro approach. These were analyzed by inhibition of protein denaturation method. Acetyl salicylic acid was used as a standard drug. Results revealedthat the petroleum ether extract of Cnidoscolus aconitifolius latex at four different concentrations influenced significant anti-arthritic activity as compared to standard used drug. The latex petroleum ether extract showed better activity. In the study, petroleum ether extract of Cnidoscolus aconitifolius latex inhibited heat induced protein denaturation and may be one of the reasons of possessing anti-arthritic activity. It can be concluded that petroleum ether extract of Cnidoscolus aconitifolius latex possess good anti-arthritic activities. The medicinal value of Cnidoscolus aconitifolius latex and make reasons why this is used traditionally for various diseases. From this study we conclude that it is promising to develop a latex based anti-arthritic drug for the ailments of rheumatic diseases and other associated diseases.
361 Evaluation of antimicrobial activity, antioxidant, and cytotoxicity of ethanolic and aqueous extracts of Tagetes multiflora Kunth chinche, David Velásquez (1), Teresa Gallardo-Jugo (2), Mónica G. Retuerto-Figueroa (1), Eva Ramos-Llica (1), María Calixto (1), Celia Vargas de la Cruz *(2), Elizabeth C. Ortega-Romero (1), Nelson Bautista-Cruz (3) Md. Shariful Islam (4)
Introduction: The antimicrobial, antioxidant and cytotoxicity activities of the ethanolic and aqueous extracts of Targets multiflora Kunth were evaluated, which were collected in the province of Yauyos department of Lima-Perú. The antimicrobial activity was evaluated by the well diffusion method on agar against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, Method: Pseudomonas aeruginosa ATCC 9027, Bacillus subtilis ATCC 6633, Candida albicans ATCC 14053 and Aspergillus niger ATCC 16404. Antioxidant activity evaluated by the DPPH method and the cytotoxic activity was evaluated by the bioassay of cytotoxicity in Artemia salina (CYTED). In the ethanolic extract, halos were formed against Escherichia coli (11.33 mm + 0.58), Pseudomonas aeruginosa (13 mm + 1.0) and Staphyloccocus aureus (15.67 mm + 1.15) strains; and the aqueous extract against the Aspergillus niger strain (13.67 mm + 0.58). Results: The Ethanolic extract showed an IC50 of 60.93 μg/mL + 0.40; and the aqueous extract 40.42 μg/mL + 0.24. The Ethanolic extract showed a LC50 of 35.568 μg/mL and the aqueous extract 386.048 μg/mL. Conclusion: The Ethanolic extract showed antimicrobial activity (against Escherichia coli, Pseudomonas aeruginosa and Staphyloccocus aureus), antioxidant activity and high cytotoxic activity; while the aqueous extract presented antimicrobial activity (against Aspergillus niger), antioxidant activity and moderate cytotoxic activity.
362 Morphoanatomical study and genomic DNA extraction for molecular characterization of Sinningia warmingii (Hiern) Chautems ‘papa madre’, Eva Ramos-Llica, Mónica G. Retuerto-Figueroa, Arilmí Gorriti-Gutierrez, César Fuertes-Ruitón, Teresa Gallardo-Jugo, Celia Vargas de la Cruz, Elizabeth Ortega-Romero, Md. Shariful Islam
Introduction: Sinningia warmingii (Hiern) Chautems (Gesneriaceae) is used in north eastern from Peru to relieve postpartum diseases and vaginal descents. The objective of the present study: to carry out morpho-anatomic analysis and extraction of the genomic DNA of the Sinningia warmingii "mother potato" species to identify the species. The sample was collected in Utcubamba-Amazonas. The morpho-anatomic study applied histological cutting techniques and genetic characterization with rbc-L, matK, ITS and trnH-psbA markers. Method: Morphologically, it is a herbaceous species, with opposite leaves, shortly petiolated, the apical: sessile, oval, symmetrical, regularly closed edges, obtuse base, and acute apex. Flowers with greenish bell-shaped calyx, lobes with acute apex; tubular corolla, pink-fuchsia and yellow inside. Pubescent capsule fruit. Anatomically, abundant filiform and glandular multicellular trichomes, anomocitic stomata and vacuoles of phenolic substances (anthocyanins and tannins) were observed in the stem and leaves. open vascular bundle The stem has woody vessels (helicollen tracheids) and open lateral vascular bundles. In the root (“papa madre”), ovoid amyloplasts and brachisclereids were recognized. Results:For the genetic characterization, genomic DNA was obtained, which was used to amplify by PCR: segments of the genes used for the bar code have been amplified with chloroplastidial markers (rbc-L, matk and ITS2) whose amplifications were positive. Sequencing has been performed with other species of the Sinningia genus that have confirmed the molecular characteristics of the genus. Conclusión: the morpho-anatomic characteristics of the studied species correspond to the Sinningia warmingii species, which with this biomolecular study confirmed its correspondence to the Sinningia genus.
363 Recent Trends of Research in Health Sciences in Indonesia , Abubakar Yaro
This edition of the Annals Tropical of Medicine and Public Health (ATMPH) special issue is selected manuscripts from several Health Sciences International Conferences in Indonesia, namely: (i) The 2nd International Conference on Medicine and Health Sciences, (ICMHS), Jember, November 30 to December 1, 2018; (ii) The 1st International Conference on Health Professional (ICoHP) conjunction with the Indonesia Doctoral Forum of Health Polytechnic, Jakarta, September 23 to 24, 2019; (iii) The 7th National Scientific Forum with Conjunction to The 1st International Conference and Exhibition on Dentistry (ICoDE), Jember, September, 06 to 07, 2019; and (iv) The 1st International Conference on Indonesian Family Planning & Reproductive Health (ICIFPRH), Yogyakarta, September, 30 to October 02, 2019. The four international conferences aim to facilitate scientific communication among scientists, practitioners, and students to share their research findings, to update their knowledge and to gain experiences in health sciences. The selection, refereeing, and editing of the manuscripts was carried out by the special issues editor team as the representative the ATMPH in Indonesia, namely: Principal Editor: Roy Hendroko Setyobudi (Malang, IND). Scientific and Editorial Board: Juris Burlakovs (Tartu, EST); Maizirwan Mel (Kuala Lumpur, MYS); Olga Anne (Klaipėda, LTU); Peeyush Soni (Kharagpur, IDN); Tsitsino Turkadze (Kutaisi, GEO); Yahya Jani (Kalmar, SWD); and Zane Vincēviča-Gaile (Riga, LVA). The Scientific and Editorial Board team is organized on an international basis, to meet the requirements of the Directorate General of Higher Education, Ministry of Education and Culture Republic of Indonesia, Scopus, Elsevier, and Web of Science - Clarivate Analytics. The selection and refereeing of manuscripts were also carried out based on the internationalization of the manuscript author team to meet diversity in the geographical distribution of authors. The publication of the selected manuscripts will be done gradually in several of ATMPH Special Issues volumes. All manuscripts was reviewed by at least two experts using the double-blind system, also passed refereeing by the publication requirements by ATMPH standard, reviewer's comments, and SI (Système International d 'Unités). The similarity test used Turnitin program (with the highest margin on 20 %), with 90 % of the references were strictly examined on 15 years at the oldest, and reflected in Google. This ATMPH Special Issue, May, 2020, Vol. 23 Issue 8 consists of 20 selected manuscripts, amount 18 manuscripts of them were the results of joint research by Indonesian and overseas scholars. In the collaboration researches, from abroad Indonesia are: Australia, Latvia, Lithuania, Malaysia, Pakistan, Philippines, Sweden, Taiwan, Timor Leste, and United Kingdom.
364 Pesticide Application and the residu on Citrulus vulgaris (Schard) , Anita Dewi Moelyaningrum, Noradilla D Oktavia, Rahayu S Pujiati, Febi D Rahmadi
Context: Residues of pesticides can affect the ecological system and human health. Aims: This study aimed to identify the behavior of the watermelon`s [Citrullus vulgaris (Schard)] farmers in applicating the pesticides. There are five principles in applying pesticides (5R): Right target of pest, the right target of the plant, right dose, right application timing or usage, right method of application (the right form of application and the use of Personal Protective Equipment [PPE]). Settings and Design: It was a descriptive observational study in C. vulgaris, farmer group to identify the application pesticides, and analysis the residue of pesticides. Methods and Material: Data were collected by interviews and observations. The Residues of pesticides in C. vulgaris, Schard and in the soil were analyzed by HPLC with Triple Quadrupole Tandem Mass Spectrometry detector (LC–MS/MS) using single point matrix based calibration at RL, in the angler bioChemlaboratory. Statistical analysis used: Descriptive observational study. Results: The result showed there were 13 pesticides used by farmers including insecticides and fungicides. All respondents did the right target of pest, however, they were not doing on the right target of plant, right dose, right application timing or usage, the right method of application (the right form of application and the use of PPE). Conclusions: Respondents that did not apply the 5R Principles. Pesticides residues, however, is still not detected on the soil (< 0.025 mg kg–1) and watermelons (< 0.01 mg kg–1). Therefore, it is still safe for agriculture and consumption.
365 Isolation of mesenchymal stem cells from mice bone marrow (mBMMSCs) from femur and tibia , Ariyani Noviantari, Ratih Rinendyaputri, Tiara D Yunindasari, Safitri Lafahtian, Khariri Khariri
Context: Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into osteocyte, chondrocyte, and adipocyte. MSCs are promising candidates for a regenerative disease therapy because of their ability to self–renewal and multi–lineage differentiation. MSCs can be isolated from bone marrow (BM), adipose tissues, Wharton jelly, placenta, dermis, and dental pulp. Aims: The aim of this study was to compare isolation of bone marrow MSCs from femur and tibia of Swiss Webster mice. Settings and Design: It was an in vitro laboratory experimental design. Methods and Material: Mice BMMSCs were isolated from mice femur and tibia bone marrow with flushing methods. Mice femur and tibia were immersed in povidone–iodine solution and alcohol 70 % to bones decontaminated and removed the attached fat and connective tissue. Both ends of the bone are cut and flushing with culture medium. The cells suspension incubated in 5 % CO2 incubator. Characterization of mBMMSCs was done by observed cells number. Differentiation potency of MSCs into osteocyte, chondrocyte, and adipocyte were tested using the commercially available kit with staining by alizarin red, alcian blue and oil red O. Statistical analysis used: SPSS with independent t–test. Results: The results showed that cells number of mice BMMSCs after first passage from tibia is higher than femur. Both of mice MSCs from femur and tibia can differentiate to osteocyte, chondrocyte, and adipocyte. Conclusions: mBMMSCs can be isolated from femur and tibia with same ability of differentiation to osteocyte, chondrocyte, and adipocyte.
366 Isolation of mesenchymal stem cells from mice bone marrow (mBMMSCs) from femur and tibia , Ariyani Noviantari, Ratih Rinendyaputri, Tiara D Yunindasari, Safitri Lafahtian, Khariri Khariri
Context: Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into osteocyte, chondrocyte, and adipocyte. MSCs are promising candidates for a regenerative disease therapy because of their ability to self–renewal and multi–lineage differentiation. MSCs can be isolated from bone marrow (BM), adipose tissues, Wharton jelly, placenta, dermis, and dental pulp. Aims: The aim of this study was to compare isolation of bone marrow MSCs from femur and tibia of Swiss Webster mice. Settings and Design: It was an in vitro laboratory experimental design. Methods and Material: Mice BMMSCs were isolated from mice femur and tibia bone marrow with flushing methods. Mice femur and tibia were immersed in povidone–iodine solution and alcohol 70 % to bones decontaminated and removed the attached fat and connective tissue. Both ends of the bone are cut and flushing with culture medium. The cells suspension incubated in 5 % CO2 incubator. Characterization of mBMMSCs was done by observed cells number. Differentiation potency of MSCs into osteocyte, chondrocyte, and adipocyte were tested using the commercially available kit with staining by alizarin red, alcian blue and oil red O. Statistical analysis used: SPSS with independent t–test. Results: The results showed that cells number of mice BMMSCs after first passage from tibia is higher than femur. Both of mice MSCs from femur and tibia can differentiate to osteocyte, chondrocyte, and adipocyte. Conclusions: mBMMSCs can be isolated from femur and tibia with same ability of differentiation to osteocyte, chondrocyte, and adipocyte.
367 Bioethanol used as topical antiseptics: pretreatment optimization of bioethanol production from tobacco industrial waste , Bekti Palupi1*, Boy Arief Fachri1, Istiqomah Rahmawati1, Ari Susanti1, Felix Arie Setiawan, Praptiningsih Gamawati Adinurani, Maizirwan Mel
Context: Bioethanol can be used for biosolvents and antiseptics material in the pharmaceutical industry. With the abundance of tobacco production in Jember, East Java, Indonesia, tobacco stalks become a promising biomass raw material for bioethanol. Aims: The purpose of this study was to determine the effect of temperature on the pretreatment process of bioethanol production. Settings and Design: Conventional pretreatment with batch system. Methods and Material: The materials used in this study include industrial tobacco waste, HCl, H2SO4, aquadest, filter paper, and aluminum foil. The pretreatment method used is chemical methods. The effect of pretreatment temperature was analyzed on the pretreatment process for the optimization of bioethanol production. Statistical analysis used: A percentage frequency distribution. Results: The test results of cellulose with H2SO4 solvents are 6.99 % at temperature 100 °C, 6.60 % at temperature 120 °C, and 4.47 % at temperature 140 °C. The test results of cellulose with HCl solvents are 6.00 % at temperature 100 °C, 6.23 % at temperature 120 °C, and 5,66 % at temperature 140 °C. Conclusions: The optimum temperature in the pretreatment process with H2SO4 for the temperature range 100 °C to 140 °C is 100 °C, with the cellulose content produced as much as 6.99 %. The optimum temperature in the pretreatment process with HCl for the temperature range 100 °C to 140 °C is 120 °C, with the cellulose content produced as much as 6.23 %.
368 ¬Implementation of gamification concept in the health complaint service system to improve the public health services system of Banyuwangi for supporting the health Indonesia program through a family approach, Diah Ayu Retnani Wulandari, Ifrina Nuritha, Vandha Pradwiyasma Widartha
Context: Gamification introduces game design elements into non game application by using competition, points, achievements, rules of play, status to encourage action through positive feedback such as motivating participation and loyalty. Aims: Now the Ministry of Health establishes an operational strategy through family approach to increase health services access in each unit. Banyuwangi support this program. The program' name is "pick up the ball, care for residents" for serving public health. This program requires the cooperation of citizens. Settings and Design: Designing citizen interaction in providing information to health workers in improving the efficiency of work of officers visiting people can be built through a public health complaint service system. Methods: Designing business processes using gamification and adopting a family approach to the flow of the public health complaints service process. Statistical analysis used: This reseach didn’t use statistical analysis but development design business process. Results: The gamification concept implemented easily so increase interaction, participation, motivation and enthusiasm of users using the system so users actively provide respond because this concept is complemented by the challenge and reward through giving of point from collected activities by adopting a family approach can facilitate posyandu cadres in their duties because every family member helping posyandu cadres in controlling the health of family. Conclusions: Design of business process using a combination of gamification concepts and family approach can make it easier to draw up a Health Complaint Service System design so that it can support The Health Indonesia Program through a family Approach.
369 Intranasal immunization with 54 kDa hemagglutinin pili protein of Streptococcus pneumonia increases mucosal and systemic antibodies, Diana Chusna Mufida, Dini Agustina, Olga Anne, Ahmad Wahyudi,Abubakar Yaro
Context: Streptococcus pneumoniae (Klein, 1884) can cause disease with high morbidity and mortality in children under 2 yr of age, parents, and individuals with low immunity. Amount one of the diseases that can be caused by these bacteria is pneumonia. To prevent the spread of pneumonia, intranasal immunization has been developed in several studies because of its ability to improve mucosal and systemic immune responses. To prevent the attachment of bacteria to the epithelial surface of the respiratory tract, it would be more effective if intranasal immunization uses a vaccine from bacterial pili protein. Aims: This study was conducted to determine the ability of S. pneumoniae bacterial pili protein with a molecular weight of 54 kDa in increasing the concentration of mucosal and systemic antibodies through intranasal administration. Setting and Design: In this study, pure 54 kDa pili protein was used as an antigen to immunize Wistar mice. Methods and Materials: Mucosal antibodies were identified by the presence of sIgA in nasal washings and systemic antibodies determined from serum IgA and IgG. Statistical analysis used: ANOVA. Results: Mice immunized with combination adjuvant–antigens had higher levels of sIgA, IgA, and IgG than other groups. ANOVA statistical tests showed significant differences in sIgA and IgA levels between rats immunized with antigen–adjuvants and other groups. However, there is no significant difference from serum IgG. Conclusion: This study showed an intranasal immunization of 54 kDa hemagglutinin pili protein S. pneumoniae increased the concentration of sIgA, serum IgA and IgG.
370 Effect of temperature on HCA from ash bagasse, Didin Erma Indahyani, Izzata Barid, Niken Probosari, Depi Praharani, Zane Vincēviča-Gaile
Context: Silica–based bioactive material can promote the nucleation of hydroxyl carbonated apatite (HCA) which roles on bone mineralization processes. The sol–gel synthesis produces nanoparticle. The small particle increases the surface area of particle that is known has high bioactivity. Sintering temperature affect properties and characteristics of the glass. Aims: The aims of the research was to know the effect of sintering temperature on HCA formation of nano bioactive silica from ash bagasse. Settings and Design: The study was a laboratory experimental with control group post–test only design. Methods and Material: Silica derived from sugarcane bagasse was used as a source of silica to synthesize bioactive glass and sodium silicate as a precursor. Sintering temperatures are 1 000 oC and 1 500 oC. Polysaccharide from seaweed was used as a binder to make plates from the bioactive glass. The plates were immersed in simulated body fluids (SBF) for 2 h, 12 h and 24 h. HCA formation was observed with Scanning Electron Microscope (SEM) and Forier Transform Infra–Red (FTIR). Statistical analysis used: Two way anova and LSD test. Results: The result of research shows that there were significantly different on HCA formation with variety of sintering temperature. Soaking time affects number and velocity of HCA formation. High sintering temperature cause increase HCA formation, but its formation is slower. The condition caused by crystal formation which produce bond more stable. .
371 The zinc syrup formula from bilih fish extract (Mystacoleucus padangensis Bleeker, 1852) as an alternatif of zinc supplementation in stunted children of 12 to 36 month , Eva Yuniritha, Mohammad Juffrie, Djauhar Ismail, Suwijiyo Pramono, Barakatun Niza
Context: The zinc syrup from bilih fish extract was made by formulating syrup with ingredient component of bilih fish which contains zinc of 27.8 mg 100 g–1 zinc, it was much higher than other food. Aims: The objectives of this research was to develop the zinc syrup formula from the extract of bilih fish as an organic zinc supplementation alternative in stunted children. Settings and Design: This laboratory experimental research was conducted gradually, from the preparation of the bilih fish, analysis zinc, and syrup formulation. Methods and Material: This experimental study was carried out by extracting fish, formulated as a suspension syrup formula, with zinc citrate, vitamin A and protein, added with simplex syrup, CMC–Na, citric acid, fruit flavorings, and food coloring. Statistical analysis used: The analysis of zinc and other nutrient conducted extract of bilih fish, conducted by Atomic Absorbant Sprectrofotometry (AAS) method, in the Research Laboratory and Integrated Study of Universitas Gajah Mada, Indonesia. Results: Zinc syrup proximate composition from the extract of bilih fish was fulfilling the requirement of local foodstuff for organic zinc supplementation which was very high (161.97 mg 100 g–1) much higher than other fish. Other than that the composition of protein, calcium, ferum, vitamin A, dan E in syrup also complete and it was in accordance with the requirement of foodstuff for supplementation. Conclusions: The zinc syrup formula of extract bilih fish, could be an alternative of organic zinc supplementation to solve zinc deficiency in stunted children.
372 The effects of Carica papaya L. juice on plasma aspartate transaminase/ alanine transaminase level and liver histopathology in paracetamol–induced mice, Fransiska Maria Christianty, Dewi Dianasari, Diana Holidah, Nargiss Lukman Hakim Salim Basyrahil, Putri Efina Tsamrotul Rizki,Laili Nurul Didik Saputri, Yahya Jani, Lili Zalizar
Context: Liver is one of the primary and largest metabolic organs in the body. The function of the liver can be disturbed and damaged by paracetamol and liver damage can be prevented with papaya (Carica papaya L.). Aims: The purpose of this study was to know the effects of C. papaya juice on plasma AST/ALT level and liver histopathology in paracetamol-induced mice. Settings and Design: Amount 25 Balb–C mice (Mus musculus) were divided into five treatment groups, there were normal, negative control, papaya juice dose (200, 400, 600) mg kg–1 bw–1 groups. The treatment was carried out for 7 d and then all mice (without normal groups) was induced by paracetamol. On the 8th d, blood was collected and all mice were sacrificed. Methods and Material: The materials used in this study include C. papaya juice, CMC Na, paracetamol, AST and ALT reagents, Hematoxylin-Eosin and used Experimental Laboratory methods. Statistical analysis used: One Way ANOVA and Kruskal Wallis with a confidence level of 95 % and followed by a post hoc test (LSD) to determine differences between groups. Results: Showed that pretreatment with papaya juice could prevent liver damage and inhibit the increase of plasma AST/ALT level and papaya juice dose 600 mg kg–1 bw–1 had significant effects on plasma AST/ALT level and liver histopathology in paracetamol-induced mice. Conclusions: Carica papaya L. juice can be used as a hepatoprotective agent.
373 Bones, scales of Upeneus sulphureus and Osphronemus gouramy increase adhesion and decrease IL-1β expression on monocytes against Streptococcus mutans, Dewa Ayu Ratna Dewanti, Pujiana Endah Lestari, Purwanto Purwanto, Erawati Wulandari, Ristya Widi Endah Yani, Sunlip Wibisono, Juris Burlakovs
Context: Adhesion and IL–1β on cells imunocompetent are important to prevent the spread of Streptococcus mutans infection to the systemic. One of the efforts is to use bones, fish scales Upeneus sulphureus and Osphronemus gouramy, they suspected contain of immunomodulator components. Aims: This study aims to analyze the effect of bone, scales of U. sulphureus and O. gouramy on adhesion activity and IL–1β expression on monocytes against S. mutans. Settings and Design: This in vivo experimental study using the post test only control group design. Methods and Material: Peripheral blood that has been layered with ficoll hypaque, then it added HBSS, Fungizone and Penstripe. The cells were placed in well microtiter plate, then they treated according to groups. Adhesion was stained with Giemza, IL–1β was stained with immunocytochemical. Cells were counted under a light microscope with magnification 400 times per 100 cells. The data obtained were analyzed using ANOVA followed by LSD test. Statistical analysis used: Post test only design and the data obtained were analyzed using ANOVA followed by LSD test. Results: There were no significant differences (p < 0.05) between Bones and scales. They both increase the adhesion of S. mutans and decrease the expression of IL–1β in monocytes. Conclusions: Bone and scales U. ulphureus and O. gouramy increase the recognition of monocytes to S. mutans and inhibit the spread of infection to systemic.
374 Quality validation of learning outcomes test instrument of core courses in Polytechnics Health Jakarta II, Indonesia, Iskari Ngadiarti1, Arif Jauhari1, Syarifah El Jannah, Joko Sulistyo1,2, Febi Dwirahmadi3
Context: The congruence of learning outcomes and learning achievement test instrument need to be appropriate so that graduates are competent. Aims: To evaluate the process of developing learning outcome tests and content validity in the Diploma Program of Polytechnics Health Jakarta II. Settings and Design: Survey, descriptive and cross sectional. Methods and Material: Collecting data about the validation of learning outcome test questions is done by looking at how the lecturer develops the learning outcomes test and the quality of the test instrument. The development of learning outcomes test questions was carried out with a questionnaire on 40 lecturers randomly selected and content validity data obtained from the exam questions and semester learning plan one expertise courses from seven majors. The questionnaire for the development of learning outcomes was modified by Munadi, while content validation used Lawshe coefficient with a validator number of five people. Statistical analysis used: descriptive quantitative analysis. Results: Development of 71 % learning test is a combination of essay and multiple–choice questions, 63 % of lecturers make question boxes, 33 % of lecturers always validate the problem with competency or learning achievement and only 22 % of lecturers adjust the questions with the indicators. The content validation ratio is 0.90 with a panel of five experts. Conclusions: The commitment of lecturers and institusions to maintain the quality of the learning outcomes test instrument that is integrated so that evidence based practice needs to be improved so that graduates quality is maintained.
375 Distribution of leptospirosis in Indonesia at 2017, Khariri Khairi, Fauzul Muna, Zainal Khoirudin
Context: Leptospirosis spreaded widely in most parts of Indonesia. The International Leptospirosis Society (ILS) data states that Indonesia was a country with a high incidence of leptospirosis and third ranks in the world. Aims: This article presents leptospirosis cases that has been reported infected humans in 2017. Settings and Design: It was quantitative study with descriptive design. Methods and Material: The data used for the analysis were reports of leptospirosis cases from the Zoonosis Program in the area during 2017 which were reported to the Directorate of the Prevention and Control of Vector and Zoonotic Diseases in the Ministry of Health. Statistical analysis used: Data were analyzed descriptively. Results: Leptospirosis cases infected human in 2017 occur in six provinces in Indonesia were DKI Jakarta, Banten, West Java, Central Java, East Java and DIY (Daerah Istimewa Yogyakarta). The number of leptospirosis cases was 908 with 136 people died. The most cases occurred in Central Java as many as 409 with 65 people died. The highest mortality rate due to leptospirosis infection occurred in East Java Province which amounted to 17.9 %. Conclusions: During 2017 there were still many cases of leptospirosis in Indonesia. The government through the Ministry of Health had sought the search and treatment of patients with leptospirosis by involving all related factors comprehensively. Leptospirosis rapid assessment after extraordinary events had carried out to determine the magnitude of health problems faced and to control efforts to break the chain of transmission. .
376 If I like it, I'm eating it: Study of high–calorie high–fat food preference among obese police officer, Septa Indra Puspikawati, Nyoman Kertia, Martalena Purba, Karina Stankevica, Wahyu Widodo
Context: Police officers must have a readiness to respond quickly and sometimes lastingly to extremely taxing physical situations so they need strong stamina and nutritious food. However, many police officers are obese because they like to eat foods high in calories and fat. Aims: This research aims to know the relationship between food preferences and food consumption of high–calorie high–fat as well as knowing the factors that relate to food preferences on police whose had a problem with obesity. Settings and Design: The design of this study was cross sectional and carried out at Madiun Resort Police Department, Indonesia. Methods and Material: The study population was all obese police officers totaling 145 police officers and we used the total population as a sampling method. The sample criteria had IMT ≥ 25 kg m–2, not in a special diet, age ≥ 24 yr, male. Food consumption was measured using Semi–Quantitative Food Frequency Questionnaire (SQFFQ). Food preference was measured using the food preference questionnaire. Statistical analysis used: This research used PR to get the result of correlation and chi–square to test of significance. Results: The majority of obese police officers like high–calorie high–fat food (56.73 %) and have overconsumption of that food (50.75 %). The most preferred of high–calorie high–fat food is fried tempeh (97.28 %), fried tofu (94.03 %), and fried chicken (86.57 %). The research showed that the prevalence risk of obese police who have overconsumption of high–calory high–fat in the group that likes high–calorie high–fat foods was 2.12 times higher than obese police who did not like high–fat high–calorie foods. Conclusion : There is a correlation between food consumption and food preference, but there is no relationship between food preferences and nutrition knowledge, income, food prices
377 Rapidly growing mass in the ear canal: A rare case of isolated superficial angiomyxoma, Siti Asmat Md Arepen1,Nor Eyzawiah Hassan1, Azlina Abd Rani, Sahrul Hitam, Mohd Khairi Md Daud
Context: Superficial angiomyxoma is a rare benign skin tumour and usually present as an asymptomatic lesion. It is known to be highly recurrent and complete wide excision is the best treatment. Aims: This cace reported of 56 yr old gentleman presented with fleshy, pink coloured right external auditory mass. The mass arisen from the posterior wall of the canal at the bony–cartilaginous junction. Clinical diagnosis of an anaural polyp was made and the mass was excised. However, it rapidly recurred bigger than its actual size within a week. The histopathological examination was reported as superficial angiomyxoma. A differential diagnosis of isolated superficial angiomyxoma should be considered in the case of external auditory canal mass and Carney complex needs to be considered. Conclusions: Complete excision and regular follow up is recommended.
378 Synbiotic and nutrients supplement improved of Secretory Immunoglobulin A (sIgA) in treated pulmonary tuberculosis patients , Suparman Samsidi, Hardinsyah Hardinsyah, Clara Meliyanti Kusharto, Ahmad Sulaeman, Bachti Alisjahbana, Vaidehi Ulaganathan
Context: Basically, bacterial populations in the gut ecosystem of healthy people who eat a balance diet are generally stable. Used of anti–tuberculosis drugs that consist of several antibiotics was alterating of the human gut microbiota. Aims: To analyze efficacy of milk–based protein supplement, synbiotic and micronutrients supplements on maintaining of microflora balance and increasing of sIgA level of treated pulmonary tuberculosis patient. Settings and Design: A double–blind randomized treatment–control trial design. Methods and Material: Data of microflora population of Lactobacillus sp. and Bifidobacterium sp. in human fecal was enumerated using plate count method and counting chambers method. Determination of secretory IgA (sIgA) in saliva was using sIgA ELISA Kit. Both subjects of treatment and control groups got same standard package of anti–tuberculosis drugs and MBP supplementation, except of treatment subjects were given synbiotic and micronutrients. Statistical analysis used: Wilcoxon signed ranks test and Mann–WhitneyU–test. Results: After first month of intervention, total colony of Lactobacillus sp. and Bifidobacterium sp. were higher in treatment group than control groups (p < 0.05). sIgA concentration in saliva of treatment group did not changes (p > 0.05) in all phase, contrary in the control group sIgA titers after 6 mo was declined (p < 0.05).Conclusions: There was positive effect of these supplements to maintain balance of gut microflora and stimulating sIgA secretion during TB chemotherapy.
379 Energy, protein, and potassium intake with nutritional status among chronic renal failure patients undergoing hemodialysis in hospital Dr. M.Yunus, Bengkulu, Indonesia, Tonny Cortis Maigoda,Esse Maisyorah,Arie Krisnasary, Sandy Ardiansyah
Context: Renal failure was the standard end line of various urinary and renal tractus. Malnutrition among hemodialysis patients can be estimated using Subjective Global Assessment (SGA)–Dialysis Malnutrition Score (DMS) which is simple and reliable. Aims: The purpose of this research was to know the relationship between energy intake, protein, and potassium with nutritional status SGA in renal failure patients who were treated by hemodialysis in hospital Dr. M. Yunus Bengkulu. Settings and Design: The design of this research was analytical observational by using prospective cohort approach. Methods and Material: The number of respondents was 57 patient. Recall method was used to measure intake of energy, protein, and potassium with 2 × 24 h recall a week, as well as the measurement of SGA–DMS. Statistical analysis used: Bivariate statistical tests using correlation are testing the significance of the relationship or difference with a 95 % confidence level and with α = 5 %. Results: There was a relationship between the protein intake (p = 0.035) with SGA, there was no relationship between the energy intake (p = 0.242) with SGA, and there was no link between intake of potassium with SGA (p = 0.603) in chronic renal failure patients undergoing hemodialysis. Conclusions: This study showed which is no significant relationship between energy intake with SGA, there was a relationship of protein intake with SGA and there was no relationship between intake of potassium with SGA.
380 Snack–based pury, safe and beneficial to maintain the lipid profile in elderly, Trina Astuti, Nur'aini Sulilo Rochani, Endang Titi Amrihati, Siti Nur Rochimiwati, Angelina Rosario Bustos
Context: Pury is a yellowish flour made from the silkworm pupae which is a sericulture by–product. It has a well–balanced amount of nutrients including PUFA (Polyunsaturated Fatty Acid), safe and does not cause allergies. Aims: The aim of the study is to find out the benefit of snack–based pury consumption in maintaining lipid profile on 30 elderly who living in nursing home. Settings and Design: Design research is a pre–experimental with the model of "one group pre–test post–test". An intervention conducted over 2 mo by giving snack for 6 d in a week. Methods and Material: elderly given daily snacks containing 10 % to 20 % of pury, 50 Cal energy and 6 g protein for 2 mo. Statistical analysis used: t–dependent paired test. Results: The result shows the average compliance is 98.8 % of snack consumption, and the proportion of elderly with normal blood lipid level increases. Analysis t test shows significant changes after the intervention of 2 mo in total cholesterol levels (t = 3.951 and p = 0.000), Low Density Lipoprotein (LDL) cholesterol (t = 2.697 and p = 0.012) and High Density Lipoprotein (HDL) cholesterol (t = 2.224 and p = 0.034), but not significant levels of triglycerides (t = – 0.698 and p = 0.491). Conclusions: Snack–based pury can be used as an alternative snack for the elderly in a nursing homes or those who live in the central areas of silk yarn industry to achieve normal blood lipid profile.
381 Performance of female nurses: Dual career, work environment and work stress, Wiji Utami, Markus Apriono, Sudarsih, Nadia Azalia Putri, Niken Probowati
Context: Performance of female nurses in several hospital in Bondowoso, Indonesia. Aims: To determine the effect of dual career and work environment on the performance of female nurses with work stress as an intervening variable. Settings and Design: The population in this study were all female nurses in several hospitals in Bondowoso namely Dr. Kusnadi Hospital, Bhayangkara Hospital, and Mitra Medika Hospital who are married and or have children. Methods and Material: The sampling technique used is saturated samples or censuses. The data used are qualitative and quantitative data. Statistical analysis used: Path analysis. Results: Based on the result of path analysis in this study, dual career has positive and significant effect on job stress, work environment has negative and significant effect on job stress, dual career has negative and significant effect on job performance, work environment has positive and significant effect on job performance, and job stress has negative and significant effect on the job performance of female nurses in Bondowoso, Indonesia. Conclusions: Demands to balance work duties as nurses and demands as family members have the potential to create work–family conflicts that have an impact on work stress and reduce performance. Uncomfortable and disturbing work environment also can be a stress trigger and then bring bad effect on nurse performance. The result showed that dual career triggers job stress and reduce performance, work environment decreases job stress and increase job performance, and job stress decreases the job performance of female nurses in Bondowoso, Indonesia.
382 Review article: The effect of borax as a food additive on energy metabolism, Yunita Satya Pratiwi, Irma Prasetyowati, Manik Nur Hidayati, Ruli Bahyu Antika, Lirista Dyah Ayu Oktafiani, Damat Damat, Nadia Shoukat, Kaleem Ahmed
Context: Borax is still widely used for food in the community. Borax can attack mitochondria and cytoplasm where cellular energy metabolism occurs. Aims: The purpose of this study was to analyze the impact of borax on energy metabolism. Settings and Design: Review article. Methods and Material: The writing method was based on scientific phenomena that occurred in the research which was then analyzed deeply based on theories taken from the scientific literature. Statistical analysis used:-----. Results: Boron ions affect the activity of at least 26 different enzymes that are needed for energy metabolism. Boric acid as active substances of borax can form stable complexes with hydroxyl compounds from glucose, protein and fat which are the raw materials for energy metabolism, and inhibit nicotinamide adenine dinucleotide (NAD+) activity which is a coenzyme in energy metabolism. Decrease in adenosine triphosphate (ATP) due to boric acid through the binding of NAD+ and an increase in thermogenic protein pathways. The ATP reduction which disrupts ion pumps and accumulation of boron ions will damage the composition of ions in the cytoplasm where the process of glycolysis occurs. This ion pump disruption will also cause cell swelling, and disrupt the balance of ions which will lead to mitochondrial dysfunction and damage (the site of the Krebs cycle and oxidative phosphorylation). Conclusions: Borax has a negative effect on energy metabolism, through the macronutrient, NAD+, enzymes, ATP, cytoplasm and mitochondria pathway.
383 Hookworm infection and the risk factors among plantation workers in Jember, Indonesia , Yunita Armiyanti, Wiwien Sugih Utami, Yudha Nurdian, Julie Ann S. Ng,Bagus Hermansyah
Context: Hookworm infection is one of the neglected tropical disease (WHO) and 428 200 000 people are infected with hookworm in wordwilde. Hookworm’s larvae requires soil media in plantation area. Contamination of plantation soil with hookworm’s larvae can occur through unhygienic defecation habit. Hence, workers in plantation areas have a high risk of being infected with hookworms. Aims: This study was conducted to determine the prevalence hookworm infection in plantation workers in five plantation areas of Jember, Indonesia District and the risk factors for hookworm infection. Settings and Design: This study was observational analytic study with cross–sectional design. Methods and Material: The prevalence of hookworm infection in plantation workers was determined based on qualitative methods (Sedimentation and Floatation Method). The defecation habits of the workers were known through questionnaires by interview method. Statistical analysis used: The characteristics of workers and the risk factors were analyzed by the Chi–square test or Fisher’s exact test. Results: The results of the study showed that the prevalence of hookworm infection in five plantation areas was 21.30 %. Based on the results of the questionnaire, most plantation workers (80.5 %) did not defecate in the toilet but at the river or the plantation area. This risk factor had significant association with the prevalence of hookworm infection (p < 0.05). Conclusions: The results of this study indicated that unhygienic defecation patterns cause hookworm contamination in the soil of the plantation area. Therefore, the plantation workers could be always expose by the infective larvae as long as they don’t change their defecation habit.
384 Effectiveness of three dimension demonstration methods for waste selection behavior children’s of the Al-Qur’an mosque education park Jabal Nur in Pajangan, Bantul, Yogyakarta, Indonesia, Zulfa Nur Fadhila1,Lucky Herawati, Bambang Suwerda1, Desy Nuryunarsih, Abubakar Yaro
Context: Waste is the result of human activities that cannot be used anymore. Waste that is not managed properly can cause disease. The volume of waste in Bantul reached 2 190 t d–1 and experienced an increase in 2016 amounting to 2 299.29 t d–1. Aims: Compare the method of demonstration of three–dimensional trash with conventional. Settings and Design: This study was a quasi–experimental design with a non equievalent control group. Methods and Material: as many as 30 respondents for each group. Statistical analysis used: Data from the study were analyzed using the test Mann–Whitney with a confidence level of 95 %. Results: The results of the study showed an increase in knowledge, attitudes and practices in the experimental group and there were differences in knowledge, practices between the experimental and control groups. Conclusions: There are different demonstration methods using three trash bins with conventional trash cans to the meaningful knowledge and practice of sorting trash from the Al–Qur'an Mosque Education Park children, while those in attitudes are not meaningful. It is recommended that the management of the Al–Qur'an Mosque Education Park use a three–dimensional trash can as an intermediary so that the children of the Al–Qur'an Education Park get used to sorting and disposing of garbage in its place.
385 The umbilical coiling index in term pregnancy as a marker of perinatal outcome, Masriyah Rashad Hussein, Rana Jasim Mohammed, Abd Aljabbar Jamee
The coil is of (360) degree spiral course of umbilical vessels. The normal umbilical cord coil is one coil 5 cm. The antenatal umbilical coiling index was intended as the equal of the distance involving a pair of coils, [antenatal UCI = 1 / distance (cm)]. The distance between the coils was measured from the inner edge of an arterial or venous wall to the outer edge of the next coil along the lateral side of the umbilical cord. Umbilical cord coiling index grouped as follows: Hypocoiled umbilical coiling index 90th percentile; Normocoiled umbilical coiling index between 10th-90th percentiles. The aim of this study is to decrease perinatal and morbidity and mortality among neonates by early detection of umbilical coiling index by ultrasound examination during pregnancy, before starting active labor and study In this cross-sectional study include (100) cases of pregnant women at early labor (latent phase) exam by ultrasound, during which umbilical cord cross-sectional region, umbilical, vessels cross-sectional area and umbilical coiling index: were calculated and compared with Doppler - parameters including- umbilical vein blood flow volume; peak systolic velocity and umbilical artery pulsatility index. The ultrasound findings were correlated with Intrapartum and neonatal outcome. In this study, the result divided according to the umbilical cord cross-sectional area and umbilical coiling index values. Our results indicated that the cross-sectional area of the umbilical cord was below the 10th percentile in 14% of fetuses, while 86% was with the normal cross-sectional area. Birth weight and placental weight were in the lower limits in fetuses with the lean umbilical cord. The most umbilical cord parameters measured by ultrasound were of lower values in fetuses with the lean umbilical cord. Measurement of the umbilical coiling index shows that in 15% of fetus’s umbilical coiling index was below normal values and 9% was above normal values and 76% was in the normal range and finds that abnormal values of the umbilical coiling index were associated with adverse perinatal outcome. There is a weak negative correlation between the umbilical coiling index and the neonatal birth weight and a moderate positive correlation flanked by umbilical vein blood flow volume and umbilical cord cross-sectional region. To conclude, the parameters of the umbilical cord that are measured by U/S were of lower values in fetuses with bend umbilical cord and most of the adverse perinatal outcomes were shown to be associated with abnormal values of the umbilical coiling index.
386 The impact of sweet basil plant additive on biochemical parameters and organoleptic quality of meat of chicken, Thair Jawad Kadhim Al-Kelabi , Mayada F. Mohamed , Hassan Al-Karagoly
This study aimed to evaluate chicken meat features after frozen when using sweet basil (SB) plant additive to work as an antioxidant. A 120 1-day old broiler unsex chicks were distributed in two groups (60 chicks for each group) and held for 42 days. The first group was control and the second group was feed additive 0.5 % SB. The study investigated some freshness, extend shelf-life and, sensory evaluation (pH, water holding capacity (WHC), thawing losses, total volatile basic nitrogen (TVB-N), Thiobarbituric acid reactive substances (TBARS) during freezing of broiler meat. There were non-significant differences in TVB-N value of breast and thigh meat in treatment and control groups. The TBARS values for treated and control breast pieces were the same, while in relation to thigh samples the treated values were significantly (P < 0.01) lower than control. The pH values were shown non-significant differences among all groups. The percentage of thaw loss of treated samples (thigh and chest) progressively decreases with a significant (P < 0.01) difference compared to control groups at the same storage time of storage (45 days). The Addition of SB improved WHC.
387 Panoramic radiographic study of mental foramen regarding the location and shape among Erbil population, Alan Haydar Mawlood Qassab
The mental foramen is a very important anatomical landmark and knowledge about it is very important, especially in dentistry, because it transfers the mental nerve and vessels. The aim of the present research was to study the position, shape, and distance of MF from surrounding anatomical structures using a panoramic radiograph of dentate adult patients. The study was carried on 867 panoramic images of patients of 20- 54 years of age. Data were collected and studied for determining the position, shape, and distance of the right and left MF in relation to the gender and age group. The most common horizontal position for mental foramen was found to be in between the apices of first and second premolar, and inferior to the apex of the related tooth. The shape was mainly round, and statistical analysis showed a significant relation with the gender and non-significant relation with the age group (p
388 Association between serum vaspin levels and diabetes in obese T2DM patients, Mohammed Ismael Dawood, Kalaf Abdullah Sayer, Mohanned H. Alwan
This hospital-based descriptive cross-sectional study was carried out in Al-Fallujah Teaching Hospital, during a one year period starting from the 1st of June 2018 and ending on the 31st of June 2019. The aim of this study was to evaluate the role of vaspin in T2D obese patients. One hundred patients having DM included in the study through non-probability consecutive sampling. The study also included 100 healthy control group. A questionnaire including the demographic information [age, gender, body mass index (BMI), education level, marital status, and occupation] was recorded. Blood samples were aspirated from each subject of the study for determination of Blood sugar, HbA1c, and serum vaspin by ELISA. The study showed the mean serum level of vaspin was significantly elevated in DM patients compared to the control group (62.77 ± 6.1 and 23.98 ± 5.9 ng/l) respectively at a P-value < 0.01. current study showed that the mean serum level vaspin was higher in DM patients especially in persons with high BMI and decreased in lower BMI persons but still higher than healthy ones (P
389 Prognostic impact of hormone and HER2 status on the prognosis of breast cancer in Mosul, Alya A. Al Zobair, Bassam I. Jasim , Barrak F. Al Obeidy, Nazar M. T. Jawher
Currant molecular classification of breast cancer based on Estrogen receptor (ER), Progesterone receptor (PR) and HER2 for gene expression is widely used for determining treatment approach for patients with breast cancer, our aims were to discover whether the molecular classification of breast cancer provides more information regarding survival compared to conventional histopathological prognostic factors. In this study we analyze the prognostic impact of ER, PR and HER2 expression using immunohistochemical study in breast cancer patients (n=152) independently and combined to gather, Survival analysis was done using Kaplan-Meier and Cox proportional hazards models adjusting for patient age, stage of the breast cancer. Molecular classification of breast cancer based on the pattern of expression of ER, PR, and HER2 was done. ER+/ PR+/ HER2– a subtype of breast cancer was found in 40.7% of cases, ER +/ PR+/ HER2 + was observed in 28% of the cases, ER-/ PR- / HER+ was found in 12.5% and finally, triple-negative subtype was found in 17.7% of the cases. Our result demonstrated that patients with different expressions of ER, PR, and HER2 have a different clinical outcome with different responses to treatment. Patients with ER+/ PR+/ HER2 – tumor have a favorable prognosis while patients with triple-negative tumors have the worst prognosis. Therefore, we recommend using this molecular classification in all patients with breast cancer for selecting the appropriate treatment approach
390 Isolation and diagnosis of bacteria causing inflammation resulting from fractures of the lower or upper jaw and treated with Olive leaf extract, Sameer H. Abdul-Haleem, Ghaidaa J. Mohammed,Ali H. M. Al-Mamoori
Background: A particular attention is given to mandibular fractures owing to the diversity of locations, severity of fractures and the accessibility of different treatment modalities. The aim of current study was to investigate the bacteria causing inflammation of the lower and upper jaws. Methods: In the present study, 134 different samples were collected for various fractures during the period from 2018 to 2019. Types of bacteria causing inflammation were isolated in cases of fractures. The laboratories of the Department of Basic Medical Sciences at the Faculty of Dentistry and by using special laboratory techniques for culturing and diagnosis of bacteria represented by the polymerase chain reaction technique and the use of special genes. Results and Conclusion: Five types of inflammatory bacteria were found in the upper and lower jaw fractures, they were as the following; Acinetobacter baumannii (353 blaOXA-51; 30.05%, Pseudomonas aeruginosa (16SrDNA; 36%, Streptococcus pyogenes (pSM19035_003 ORF alpha; 30%, Staphylococcus aureus (ORF28 similar to putative transposase; 17%. The study found that there were significant differences with different bacterial species with infection rates. The effect of olive leaf extract was also observed, there was a significant effect among bacteria with olive leaf extract. Concentrations of 10%, 20%, 30%, 40%, 50%, 60% and 70% were used. There was a significant moral difference.
391 Assessment Pregnant Women’s practices about antenatal care during Pregnancy at Primary Health Care Centers in Kirkuk City, Iraq, Iman Salman Hassan, Hanaa A. Omer
Background: Antenatal care (ANC) is done to prepare a pregnant woman for birth and motherhood as well as prevent, detect, alleviate, or manage the three types of health problems during pregnancy that affect mothers and babies. These health problems include i) complication of pregnancy itself, ii) pre-existing conditions that worsen during pregnancy, iii) effects of unhealthy life style. The aim of current study was to evaluate pregnant women's practices about antenatal care during pregnancy in primary health care centers in Kirkuk City after implementation of a program. Methods: A quasi-experimental design was used in the present study with the application of a pre-test/ post-test approach for assessment of study group. The study was conducted between 2/12/2018 to 6/10/2019. Non- probability (purposive) sampling method was adopted to select 100 subjects, 50 study group and 50 control group, from pregnant women in first trimester who visited Primary Health Care Centers in Kirkuk City. Results: The results showed the distribution of studied socio-demographical characteristics variables where high number of subjects in study group with age 25-29 years (36%), level of education was Institute and college graduate (30%), Occupation was Housewife (66%), Monthly Income was (58%), Residential Area was urban (78%), with Smoking Status was non-smoking (72%). The results also showed age at marriage was 20-24 years, 42 % of study group period were married while 38% of control group. Also, both groups showed the highest percentages (68% and 88%, respectively, were menstruation, number of pregnancies for both groups (52% and 54%), and (88%, 92%) for both group were from number of abortion. Regarding the para number of babies were both group (48%, 56%), respectively, while type of delivery for both groups same percentage (56%). Heredity diseases, study and control group (86%, 98%), while health problems same percentage of both group (78%). The results showed that there were no significant differences at P>0.05 accounted between study and control groups regarding all items related to practices of ANC.
392 Association of Interleukin-10 Gene Polymorphisms and serum levels with susceptibility to infection with Hepatitis B Virus, Farah T. Hasan, Hassan M. Naif, Zaid H Taha, Safaa A. Abdul-Razzak
Background: Hepatitis B Virus infection is a major public health problem with 30% of the world’s population are infected. The aim of current study was to investigate the association of interleukin-10 promoter gene polymorphisms and its serum levels in patients with susceptibility to HBV infection. Methods: The polymorphisms 10-1082A/G and -819C/T and their serum levels were determined using 200 individuals by allele-specific PCR and ELISA, respectively. Results: Interleukin-10-1082 GG, AG/GG genotypes and G allele frequencies showed a strong risk association with HBV infection (OR; 1.287; 95% CI, 0.87-1.63; P=0.009; 1.348 (0.89-1,63), P=0.0063 and OR: 3.296; 95% CI, 0.93-1.54; P=0.0068, respectively) and enhanced the circulating levels of interleukin -10 significantly (P=0.004). On the contrary, interleukin -10-819 TT genotype and T allele were not correlated with the risk to HBV infection (OR: 0.685; 95% CI, 0.87-1.62, P=0.046; and OR: 0.863; 95% CI, 0.92-1.57, P=0.863, respectively) but the CT/TT had a significant association (OR: 1.652; 95% CI, 0.92-1.62, P=0.0001) which resulted in a significant inhibition of interleukin -10 production (P=0.005). A significant association between HBV and the haplotype GC (P=0.0073) and GT (P=0.0326) was also observed. Conclusion: These results suggest that gene polymorphisms of interleukin-10-1082 and -819 had differential effects on disease risk association and on interleukin-10 serum levels in HBV infected patients.
393 Effect of Iron relation with antioxidants on Endometriosis in red meat consumers females, Nawal M. J. Al- Shammaa
Background: Iron has several vital functions in the body. It serves as a carrier of oxygen to the tissues from lungs by red blood cell haemoglobin as a transport medium for electrons within cells and an integrated part of important enzyme systems in various tissues. The aim of current study was to correlate iron with antioxidant and some biochemical parameters such as serum iron, TIBC, Hb, BMI,UIBC and ESR in endometriosis red meat consumer females. Methods: The present study was conducted on 36 females with nutrition on red meat in addition to 36 healthy females. All subjects were (20-40) years old. Parameters were measured in sera of Endometriosis females, with nutrition on red meat, including body max index, Hemoglobin, Serum Iron, Total Iron Binding capacity, unsaturated Iron binding capacity, PCV, ESR and antioxidants such as 8-Hydroxy-2-deoxy guanosine, maloaldehyde acid. Results: The results revealed significant elevations in all parameters of patients sera compared to healthy group. Conclusion: Patients who ingest a lot of red meat have high levels in serum iron and low in TIBC were a markers or player due to diseases like liver disease, osteoarthritis hypertension, Hemochromatosis and cardio vascular diseases.
394 Postpartum level of HbA1c and risk factors of delivering a large baby, Maysam Hatem Khaleel , Hanan Hazim Mohammed Noori, Marwa Jaafar Sadac
Background: A mother who had abnormal glucose tolerance during pregnancy may lead to delivery of a large baby. Glycosylated hemoglobin concentration might be expected to identify women who had high blood glucose concentration before delivery. The aim of current study was to identify retrospectively gestational diabetes in mothers of large babies. Methods:A prospective case-control study that was conducted in the Department of Obstetrics and Gynecology at Al-Imamine Al-Kadhimein Medical City, Baghdad, Iraq for a period of one year and included 100 women who recently delivered newborns and divided into two groups; Macrosomia group included 50 women who delivered babies weighed 4000gm or more and control group included 50 women who delivered babies weighed less than 4000gm.Women who were known cases of diabetes, blood disorders, delivered a preterm baby, delivered a baby with any congenital malformations, or still birth delivery were excluded from this study. Blood was obtained from all women within 72 hours after delivery to investigate for HbA1c level. A questionnaire was applied to all women to collect the needed information. Results: The prevalence of macrosomia was significantly increased with increasing age, and parity of mothers, gestational age ≥40 weeks, increasing body mass index level, positive history of macrosomia, and positive family history of diabetes. Mean HbA1c was significantly higher in women who delivered macrosomic babies than those who delivered babies with birth weights5.8% was predictive for macrosomia.Conclusion:HbA1c is a good indicator of unscreened or unobserved glucose intolerance in mothers who delivered a macrosomic baby. Other possible risk factors for macrosomia included aging, increased parity, obesity, previous history of macrosomia, and family history of diabetes.
395 Postpartum level of HbA1c and risk factors of delivering a large baby, Maysam Hatem Khaleel , Hanan Hazim Mohammed Noori, Marwa Jaafar Sadac
Background: A mother who had abnormal glucose tolerance during pregnancy may lead to delivery of a large baby. Glycosylated hemoglobin concentration might be expected to identify women who had high blood glucose concentration before delivery. The aim of current study was to identify retrospectively gestational diabetes in mothers of large babies. Methods:A prospective case-control study that was conducted in the Department of Obstetrics and Gynecology at Al-Imamine Al-Kadhimein Medical City, Baghdad, Iraq for a period of one year and included 100 women who recently delivered newborns and divided into two groups; Macrosomia group included 50 women who delivered babies weighed 4000gm or more and control group included 50 women who delivered babies weighed less than 4000gm.Women who were known cases of diabetes, blood disorders, delivered a preterm baby, delivered a baby with any congenital malformations, or still birth delivery were excluded from this study. Blood was obtained from all women within 72 hours after delivery to investigate for HbA1c level. A questionnaire was applied to all women to collect the needed information. Results: The prevalence of macrosomia was significantly increased with increasing age, and parity of mothers, gestational age ≥40 weeks, increasing body mass index level, positive history of macrosomia, and positive family history of diabetes. Mean HbA1c was significantly higher in women who delivered macrosomic babies than those who delivered babies with birth weights5.8% was predictive for macrosomia.Conclusion:HbA1c is a good indicator of unscreened or unobserved glucose intolerance in mothers who delivered a macrosomic baby. Other possible risk factors for macrosomia included aging, increased parity, obesity, previous history of macrosomia, and family history of diabetes.
396 Mechanical dyssynchronyin dilated cardiomyopathyand relation to severity, etiology, and ECG, Ahlamkadhim Abbood, Haider Jabbar Al-Ghizzi, Zainab F. Hassan
Background: Cardiac impairment is a maincause of mortality throughout the worldand despite optimal medical treatment, it still carries high mortality. The aim of current study was to evaluate the intraventricular dyssynchrony) prevalencein patients with dilated cardiomyopathy patients and its relation to underlying etiology, QRS with and severity of systolic dysfunction.Methods: This cross-sectional study included 58 patients with cardiomyopathy of ischemic (ICM) and non-ischemic cause (NCM) . Mechanical dyssynchrony (intraventricular dyssynchrony) assessed by m-mode, pulsed Doppler, TDI and 2D speckle tracking.Assessment of left ventricular systolic function was done by ejection fraction and stroke volume. Prevalence of mechanical indices in DCM and their relation to underlying etiology, QRS duration and severity of LV systolic dysfunction were done. Results: Prevalence of intraventricular dyssynchrony)indices were more in NCM than ICM except PSI was more in ICM,and there was significant association of these indices with QRS width. There was non-significant negative correlation between these indices and LV systolic indices. Conclusion: Mechanical dyssynchrony)indices are affected by the underlying etiology and QRS width as well as severity of LV systolic dysfunction associated with existence of the mechanical dyssynchrony.)
397 Letrozole ovulation induction in clomiphene citrate poor responders polycystic ovary patients, Mysoon Shareef, Manal Nasih Ahmed Hamdan
Background: Polycystic ovary syndrome is one of the most common endocrinopathy affecting women of reproductive age group with a prevalence of 12-18%. It is a complex syndrome with reproductive, metabolic and psychological features. The aim of current study was to evaluate the effectiveness of use of letrozol (aromatase inhibitor) in induction of ovulation in cases of clomiphine citrate poor responder Polycystic ovary syndrome patients. Methods: Prospective randomized study carried out during the period from January 2008 until April 2011 in Basrah infertility center. In this study 83 patient with primary infertility due to PCOS were treated by clomiphine citrate for 3-6 months. Those who failed to ovulate with clomiphine citrate were given Letrozole in a dose of (2.5-5mg/day from the 2nd to the 6th day of menstrual cycle. When the dominant follicle reached a diameter of 18mm, HCG 10,000IU was given and timed intercourse was advised. Results: In the day of HCG administration, the mean number of follicles ≥18mm was 1.2 (range was 1-2) and the mean endometrial thickness was (9.40±1.3mm). Ovulation occurred in 29 cases (75%) and pregnancy in 9 cases. Conclusion: Letrozole is associated with a higher pregnancy rate in polycystic ovary syndrome patients than clomiphine citrate. It has limited number of mature follicles with less chance of hyperstimulation or multiple pregnancies. In addition, induction of ovulation with Letrozole is associated with no adverse effect on endometrium or cervix. It is safe, easily administrated, cost-effective and may be a good alternative for clomiphine citrate poor responder cases before using expensive and risky gonadotropins.
398 THE EFFICACY AND SAFETY OF ULTRASOUND GUIDED PERCUTANEOUS DRAINAGE OF PYONEPHROSIS DURING PREGNANCY, Wadhah Adnan Al-Marzooq, Jawad abdalhassan Mseer, Hassan Falah Hassan
Background: Upper urinary tract obstruction during pregnancy carries high risk of maternal and fetal complications. The most serious complications are infection and urosepsis, abortion or premature labor, and intrauterine fetal death. Studies that involved non-infected upper urinary tract obstruction during pregnancy recommend the use of either a retrograde passage of JJ stent or a guided percutaneous nephrostomy. The aim of this study was to assess the efficacy and safety of percutaneous nephrostomy in treatment of septic obstruction of the upper urinary tract during pregnancy. Methods: The present study involved 21 pregnant woman with unilateral obstructive uropathy, 9 of theme treated by inserting an ipsilateral double J stent, whereas 12 patients treated by ultrasound-guided percutaneous nephrostomy. Results: All patients treated by nephrostomy got clinical improvement within the first 48 postoperative hours and all of them completed their pregnancy and delivered full term baby by normal vaginal delivery. Conclusion: We conclude that ultrasound-guided percutaneous nephrostomy catheter drainage of infected obstructed upper urinary tract during pregnancy is a safe, effective and life-saving procedure and considered as first line of drainage in such patients.
399 Inhibitory activity of Saccharomyces cerevisiae, Rhodotorulaglutinis and Lactobacillus spp against Escherichia coli isolated from children diarrhea infection , Fahad K. Y. Al-Dulaimi, Janan K.Al-Tarjuman, Faten N. Mulla Abid
The study was conducted to demonstrate the inhibitory activity of Yeast and Lactobacillus spp. Escherichia coli isolated from children's diarrhea Infection. Yeast isolates of Saccharomyces cerevisiae were isolated from dried date, Rhodotorulaglutinis was isolated from apple fruit. Pure cultures were prepared on Sabouraud's dextrose agar (SDA). Lactobacillus spp. were obtained from Lacteol fort Sachets. A yeast and Lactobacillus spp isolates have been prepared and its antibacterial activity against E. coli. Our Results show variable effectiveness were observed, Lactobacillus spp had the highest inhibitory activity against E. coli, while Yeast interaction that the highest Inhibitory activity from yeast signal against E. coli.In conclusion: the results of S. cerevisiae, Rh.glutinis and Lactobacillus spp isolates showed inhibitory activity against E. coli.
400 Detection of Pneumocystis jirovecii by Grocott-Gomori Methenamine Silver and Indirect Immunofluorescence Assay in Chronic Obstructive Pulmonary Disease (COPD) Patients, Jabbar S. Hassan, Abdul-Jabbar K. Ibrahim, Ahmed E Salman, Qasim S. Al-Mayah
Background: Pneumocystis jirovecii has emerged as an opportunistic fungal pathogen in immunocompromised patients. The organism has a tropism to colonized lung in patients with chronic pulmonary obstructive disease (COPD) even with no obvious immunosuppressive state. Aims: This study aimed to investigate the colonization of P. jirovecii in patients with COPD and the association of different demographic characteristics with this colonization. Patients and Methods: Sputum samples were collected from 100 patients with COPD. Samples were processed according to the standard protocols and were examined by two laboratory techniques: Grocott-Gomorimethenaminesilver (GMS) and indirect immunofluorescence assay (IFA). Data including age, gender, smoking status and hospital stay were extracted from patients through direct interview. Results: The overall detection rate of P. jiroveciifrom COPD patients using GMS and IFA was 8% and 9% respectively. Older ages and smoking were associated significantly with increased risk of P. jiroveciicolonization in COPD patients. Conclusions: These data indicate the importance of P. jirovecii as opportunistic pathogen in colonization of the respiratory tract of patients with COPD. Further studies are required to illustrate the clinical implication of this colonization.
401 Molecular and Immunological test for detection of Toxoplasma gondii in pregnant women and patients with prostate cancer , Nihad Khalawe Tektook ; Huda Hamid Al-Janabi ; Omar Mahmood Shakir
A cross-sectional study had achieved in Baghdad government from March 2016 to March 2017”. 100 patients with prostatic cancer were involved in this study. The age of the patients was between (30-75) years, control bunch who were coordinated to the patients, including100 pregnant patients and 100 solid people (blood givers). Five ml of blood have gathered from every patient, and the control gathering took a crack at this examination. Blood tests were set into two cylinders; one of the tubes was contained anticoagulant EDTA for an atomic trial of Toxoplasma gondii, another tube was 2 ml have centrifuged. “The obtained sera was aspirated, and stored for identification of specific Toxoplasma gondii IgM and IgG by using ELISA technique”. “The study found that 40% of prostate cancer patients were positive to T. gondii by RT-PCR compared with 30% in pregnant patients and 13% of non-diseased (control). The study revealed that 55% of patients with cancer of prostate with result +ve ELISA was also +ve by PCR compared with 0% of patients with –ve ELISA results, Current study that appearance 60% of cancer of prostate patients with T. gondii IgM+ IgG+ and 58.33% IgM+ IgG- antibodies were positive by PCR. “Our study revealed that the high rate of T. gondii infection” “was founded among patients with prostatic cancer older than 70 years (53.86%) and low rate” (20%) was within patients between (30-39) year and we were observed, “That Toxoplasma gondii infection increased proportionally with increase of patients age. In conclusion, a high rate of prevalence of T. gondii DNA (as detected by RT-PCR), as well as its antibodies, have observed in prostate cancer and RT PCR was the precise manner in the detection of T.gondii.
402 Children and post-traumatic stress disorders, Rifaie Yaseen Hameed; ThairaIdress Younis
Post-traumatic Stress Disorders is recognized as a wide-spread disorder among children and adolescents world-widely in developed and developing countries. The study aimed to identify and classify the chief complaints of PTSDs among children, and present the relationships between complaints and sources of psychological pressure the children exposed to. Furthermore, our study depended on a cross-sectional, descriptive, and correlational study design to accomplish its aims. It carried out for a period from 5th of October 2017 till 30th of April 2018 on a sample of (595) children between (6 – 8) years old obtained depending on non-probability sampling technique from fourteen primary schools at the left side of Mosul city. The instrument of the study composed of physical complaints, Psychological complaints, and Cognitive complaints. Data were gathered by sending the questionnaire with the students to their mothers to fulfill them. We found that the exposure to negative life events was the main source of psychological pressure children exposed to, suffering from psychological and physical complaints were more than cognitive complaints.
403 Prevalence of hypertension and associated factors in arthritis patients attending the rheumatology department in Al-Zahraa teaching hospital in Kut city, Iraq, Sameeha NaserAbed, Wasan Talib Abed, Jenan Ali
The frequency of hypertension and their risk factors among arthritis patients attending rheumatologydepartment in a Al-Zahraa Teaching Hospital in Kut city, Iraq , especially in relation to gender, age, BMI , family history of hypertension, and disease activity score and lipid profile.Thehospital-based cases- control study was conducted for patients with arthritis patients attending rheumatology departmentfor a period of 2months from the 1st January to 1stMarch 2019. Arthritis patients with hypertension were cases and those without were controls. Among the arthritis patients recruited for this study, 33.3% were males and 66.7% were females. Among these arthritis patients, 25.6%% of male patients were diagnosed with hypertension as opposed to 74.4% of female patients. Majority of arthritis patients were from urban (62.8%%) and rural (37.2%).Hypertension were most prevalent in the age group more than 50 years (62.8%) and least prevalent in the age group 41-50years (0%). The mean HDL, total cholesterol, LDL and VLDL were all higher for arthritis patients with and withouthypertension.There are a significant changes in gender, family history of hypertension, and disease activity score between the study groups (P
404 Serodiagnosis for brucellosis in camels by rose Bengal and C-ELISA test in Iraq, Asaad Chasib Alatabi, Khaleel Z. K. Al-Alo , Abdulameer A. Hatem
Brucellosis is an occupational disease for veterinarians, farmers, and meatpacking workers. It causes heavy economic losses in livestock in developing countries and has an important public health issue for dairy product consumers. Little information is known about the camel brucellosis and its effect on human health. A study of the seroprevalence of camel brucellosis had been conducted in Al-Najaf province during the period from February 2018 to March 2019. One hundred seventy-two camels were involved in this study with ages between 3 to 9 years. The serum samples were screened by using the Rose Bengal plate test (RBPT) with positive samples tested again with the competitive ELISA test (cELISA). Our results show that out of 172 camel sera collected, 6 (6.97%) were positive for Brucella antibodies by Rose Bengal plate test of which 4 (4.6%) was confirmed by cELISA. Furthermore, on age distribution, for RBPT, the positive results were 4 camels (4.6%) and 2 camels (3.1%) for older and younger animals respectively, while in cELISA, 3 (3.4%) older camels were positive with only 1 (1.1%) younger camels. The result was statically significant (P
405 Measurement of vitamin-D in sera of children with epilepsy and its relation with anticonvulsants administration , Bassam Taha Saleh , Ayied Mutob Turki , Abdulsattar Hussein Abdullah, Mohammed Khalid Kzar
The study had conducted in Tikrit city for the period from January to June 2018 on 33 children with epilepsy and under treatment with anticonvulsant with age group 1-12 years for measurement of Vitamin-D in sera of children with epilepsy. Based on the clinical signs of patients and diagnostic tests, the number of these children had assigned to the present study. The study also included 40 healthy children as a control group. The study included the collection of 3 ml of venous blood at the beginning of the study and 3 months after anticonvulsant administration for identification and measurement of vitamin D by using ELISA technique (Koma-Biotech, Co, USA). The study also included the taking of full information from cases like living situations, age. The study showed that 90.91% of children with epilepsy have vit D deficiency compared with 12.5% of healthy control (P: 0.001). The study showed that the lowest mean of vit D had recorded in children with epilepsy as compared with healthy control. The study showed no significant relation of the age of children with the frequency of epilepsy and showed that the lowest mean of vitamin-D was recorded in children with epilepsy whose ages were below 3 years (7.33±0.9 ng/ml) (P
406 Comparative Study between Emergency and Elective Cesareansection: Indications /Complications, A Hospital Based Study , Nabila Kamil Yaquab, Ruqaya Subhi Tawfeeq, Inas Yassen Mahdi
Cesarean section is a major, open abdominal procedure, often performed in an emergency, elective setting there are a number of immediate and delayed complications that may happen. In the current study, we aimed to assess the causes of induction of CS and to identify the complications that occurred during the operation and post-operation. A descriptive study was performed from 13/12/2017-3/12/2018, 100 pregnant women were participating in this study who attended public and private hospitals for the Cesarean section in Tikrit city. Our results show that the fetal distress which was account 40% as emergency indication for C/S regarding the indications for elective cesarean section, previous 2, 3,4,5 scars were account 20%,8%,6%, and 4% respectively, about intra-operative complications due to elective C/S, uterine atony account 20% of these complications, and injury of small vessels account 10% while for intra–operative complications due to emergency C/S, bleeding due to placenta previa account 34% and uterine atony was account 16% and many types of complications were encountered during study period.
407 The immunological response toward botulinum toxin in individuals with facial rejuvenation, Saygin Abdulkadir Chakmakchy , Aasem Mohamed Al-Byti , Abdulrazzaq Abbas Waheeb
The study was conducted in the cities of Tikrit and Kirkuk for the period from December 2016 to February 2017. The study included 60 people who had previously had facial procedures, both male and fem ale, aged between 15 and 50 years to determine the levels of interleukin 2, interleukin 6 and interleukin 8 in individuals with facial rejuvenation by botulinum toxin. The study included those who did not suffer from chronic diseases, immunodeficiency or hypersensitivity for any reason. The study also included 40 healthy and healthy people from all diseases from blood donors and the same age group of the person in question. The study included all information from people in the study, such as age, sex, marital status, smoking, family members, living conditions and concomitant diseases. All people undergoing cosmetic surgery were Botox injectors. The study involved taking 3 ml of blood where the serum was isolated and saved for detection and measurement of levels of interleukin 2, interleukin 6 and interleukin 8 by ELISA technique. The current study showed 50% of each of males and females of patients under butux were within 25-34 years. In this study serum interleukin-2, IL-6 and IL-8 were significantly elevated in cases as compared with control ones (P
408 The primary trocar insertion in laparoscopic cholecystectomy after cesarean section scar: A comparative study between Hasson Technique and Verres Needle, Jalil Ibrahim Awaid alrubaye, Hussein Ali Abed Ahmad, Malik Gata Ressan Al-hashimi
A Prospective study in AL-Zahra teaching hospital of Iraq from May 2011 until May 2014 on 623 female patients, all of them have previous Caesarean section scar underwent laparoscopic cholecystectomy. The patients were randomized into two groups. Group A (307 patients), the Hasson cannula was used and group B (316 patients), were the Veress needle used. Variables comparing both methods were studied included the age, the time is taken to establish pneumoperitoneum, the number of attempts of insertion and conversion to an alternative method, conversion to open cholecystectomy and causes of conversion in both methods was studied regard the primary port access only. Majority of the patients were middle-aged (mean age 41 years), Conversion to open cholecystectomy occur in 22 patients (3.5%) in both groups. Short mean access time was achieved with the Veress needle (6.6minutes) compared with (9.5minutes) in the Hasson method. Conversion to an alternative technique occurs more in Veress needle 21 patients (6.6 %), and 3 patients (1 %) in the Hasson method. Conversion to open cholecystectomy occurs more in Veress needle 12 (4.4%) and 8 (2.6%) with the Hasson method. The major causes of conversion was severe adhesion of previous caesarian section scar (57%) followed by bowel and omentum injury (28%), obesity(7%) and Gas leakage( 7%) in Veress needle while, Gas leakage(50%) , obesity(37.5%) and severe adhesion of previous Caesarean section scar (12.5%) in Hasson method.
409 DYSLIPIDEMIA IN EARLY SECOND TRIMESTER IS MAINLY A FEATURE OF WOMEN WITH EARLY ONSET PRE-ECLAMPSIA, Ola Khalid Abdul Alhameed
To investigate whether hypertriglyceridemic dyslipidemia is a risk factor for risk factor for either early or late onset pre-eclampsia. Design: Prospective cohort study. It was carried out in AL.ZAHRAA maternity and pediatric teaching hospital in AL-Najaf during period between 15th of march to 10th of October 2011. Participants: 200 pregnant women. Blood samples were obtained from non-fasting subjecting at 18 weeks of gestation. All samples were analyzed for triglycerides, total cholesterol, low density lipoprotein and high density lipoprotein. Thirty six women developed early onset pre-eclampsia and thirteen women developed late onset pre-eclampsia. In the cohort model, women with triglycerides above 2.4 mmol/l had increased risk of early onset pre-eclampsia compared with those with triglycerides level< 1.5 mmol/l.
410 ASSOCIATION OF INTERLEUKIN 4-C590T GENE POLYMORPHISM WITH NEPHROTIC SYNDROME IN CHILDREN, Noor A. Malik, Ahmed Abdul-Hassan Abbas, Shatha Hussein Ali
Nephrotic syndrome is one of common glomerular disorder in children.Response to steroid therapy maybe associated with interleukin 4 promoter site gene polymorphisms.The aim is to investigate the role of IL-4 –gene polymorphisms in disease predisposition. Sixty patients of nephrotic syndrome (30 of them were steroid sensitive and the other 30 Patients were steroid resistant)with thirty apparently healthy individuals as control group were participated in this study. Blood samples were collected from patients and controls and DNA extraction was made from each blood sample then single nucleotide gene polymorphisms were investigated by using restriction fragment length polymorphism technique (RFLP) and ELISA technique was used to detect the serum level of IL-4.The heterozygous genotype (CT) of IL-4 C590T gene polymorphism was slightly more frequent among patients than controls without significant difference. Serum levels of IL-4 among patients show elevated values as compared to control group.IL-4 may affect response to the steroid therapy in nephrotic syndrome patients.
411 ROLE OF PSEUDOMONAS AERUGINOSA POLYSACCHARIDE AGAINST ASPERGILLUS FUMIGATUS IN MALE RATS, Saif T. Jasim
The objective of current research was designed to show the effect of polysaccharides from Pseudomonas aeruginosa against Aspergillus fumigatus toxicity. 20 adult male rats were used and distributed as follow (5 rats in each group); control group received normal diet, second group administrated (intranasal) of A. fumigatus conidid (107 conidia/ml normal saline.). Third group: infected with A. fumigatus and treated by with 150 µg/20g b. wt. of Polysaccharides for two weeks. Fourth group: infected with A. fumigatus and treated by with 250 µg/20g b. wt. of Polysaccharides for two weeks. Levels of Malondialdehyde (MDA), glutathione (GSH) and catalase show high significant changes (P < 0.05) in an infected (with A. fumigatus) group compare with control. After using polysaccharides, levels of MDA, GSH and catalase in third and four groups show non-difference (P < 0.05) compare with control. It was concluded that polysaccharides from Pseudomonas aeruginosa have been potential role against A. fumigatus.
412 ASSOCIATION BETWEEN SINGLE NUCLEOTIDE POLYMORPHISMS OF VITAMIN D RECEPTOR GENE AND DIABETES WITH PROGRESSION OF BENIGN PROSTATIC HYPERPLASIA, Rawaa AlChalabi, Mohammed H. Wali, Dhafar N. Al-Ugaili , Hayder AlAmery
Benign overgrowth of prostatic tissue represents the most frequent urological diseases in aging men. Vitamin D plays an important role in the regulation of the growth and differentiation of diverse cell types through the cell's specific receptor. The interaction of vitamin-D with VDR is thought to affect the development of BPH. This study was designed to reveal insight into the impacts of the correlation between vitamin D receptor gene polymorphism and diabetes with the expansion of BPH in Iraqi men. Four SNPs in the VDR gene were genotyped for 174 BPH patients and 171 healthy controls. Results reported that the danger of BPH increased with age. Biochemical tests gave a high serum level of PSA, RBS, and HbA1c with mean level (11.14 mg/dl, 247.2 mg/dl, and 8.6%) respectively in patients when compared with the healthy group. Patients expressed a low mean serum level of Vitamin D (7.67ng/ml) while the mean serum level of healthy individuals (34.97ng/ml). Hyperglycemia and vitamin D deficiency are strongly associated parameters that led to an increase of the prostate volume and excess a risk of the BPH progression. Diabetes and some SNPs in the VDR gene such as rs3782905 (Intron4, C/G),rs1544410 (Intron 10, G/A) rs7975232 (Intron 10, A/C) and rs731236 (Exon 11, T/C) were analyzed, the SNPs in non-coding region reflected the significant association with decreasing vitamin D level while rs731236 located in coding region provides non-remarkable effect. Diabetes and SNPs in the non-coding region considered potential markers to maximize the risk of BPH in the Iraqi population. Thus, they may be used in the future as a therapeutic goal in patients with BPH.
413 SYNTHESIS OF SOME NEW THIAZOLIDINONE COMPOUNDS DERIVED FROM SCHIFF BASES COMPOUNDS AND EVALUATION OF THEIR LASER AND BIOLOGICAL EFFICACY, Rabiha Hameed Saleh1,Wissam Mohammed Rashid2, Adi, Hussein Dalaf, Khalid A. Al-Badrany, Omar Abdulrahman Mohammed
In this work 6-fuloro-2-aminobenzothazol compound (R1) have been prepared from the reaction of aniline with appropriate potassium thiocyanate in presence cooled glacial acetic acid in such a way to temperature not exceeded above room temperature, Solution of 1.6ml of bromine in 6ml of glacial acetic acid, the reaction 6-fuloro-2-aminobenzothazol of with Hydrazine hydrate 6-fluoro-2-hydrazineylbenzo thiazole yielded compounds (R2). The research included synthesis of schiff base from the reaction of with appropriate aromatic aldehydes in presence of glacial acetic acidyieldeds compounds (R3-R7) 2-(2-benzylidenehydrazineyl)-6-chlorobenzo thiazole, and synthesis of thiazolidinone from the reaction of thioglycolic acid with schiff base,yieldeds compounds (R8-R12) chloro-N-(5-phenyl-2,5-dihydro-tetrazol-1-yl) benzo thiazol-2. The prepared compounds have been characterized by melting points and some physical properties besides the FT-IR, H-NMR spectra and quantitative analysis of elements (C.H.N.). The purity for these compounds was checked by TLC. The study is showed biological activity for chemical compounds, at three concentrations (10-2,10-3,10-4) mg/ml The minimum inhibitory concentration [MIC] have been determined with the reference of stander drugs the results showed that the thiazolidinone derivatives are better than growth of both types of bacteria gram-positive and gram–negative compared to drug, and evaluation of laser efficacy.
414 ZnO nanoparticles activity against the virulence gene of Pseudomonas aeruginosa isolated from patients with burn wounds infection in Al Muthanna population, Yasir Adil Jabbar Alabdali, Hawraa. F. Wali, Ayad. F. Alkaim
Pseudomonas aeruginosa has various virulence factors which involve in the pathogen toxicity and invasion. Furthermore, patients suffering from burn wounds are more susceptible to infected with Pseudomonas aeruginosa. Two hundred clinical swabs were collected from burn wounds in Al Muthanna hospitals, followed by routine laboratory diagnosis such biochemical tests, and then isolates were confirmed by culturing on CHROMagar™ media, followed by VITEK- 2 compact system test.Fifty strains of P. aeruginosa was diagnosed and then were tested for antibiogram. These isolates were drug-resistant against ciprofloxacin,penicillin,amikacin,gentamycin, cloxacillin, ampicillin, vancomycin, while were sensitive to piperacillin. The gDNA of P. aeruginosa isolates were extracted and then multiplex PCR was performed to detect for algD, lasB, plcH, plcN and exoS genes. The results showed the prevalence rate for these genes in P. aeruginosa isolates were; algD (100%), lasB (95%), plcH (100%), plcN (90%) and exoS (95%). The conventional PCR was used to screen for virulent genes before and after the ZnO nanoparticles treatment as a test to the antibacterial activity of the ZnO nanoparticles against these virulence genes. The results showed that ZnO nanoparticles on gene expression of those virulence genes at percentage were algD (83%), lasB (100%), plcH (50%), plcN (100%) and exoS (80%).
415 The Role of Ultrasound In The Management Of Fractured Nose, Raid M. Al-Ani, Alaa Yass Abed, Bashar Adnan Munshid, Shameem Ali Hussein
Background: the nose is the most prominent part of the face so it is more liable to be injured in an accidental or assault types of facial trauma. Radiological assessment plays a role in the management of fractured nose. Objectives: 1. To compare the ultrasound image with plain X-ray in the diagnosis of nasal fractures. 2. To compare the findings of ultrasonography at the time of presentation and after the oedema subsided in patients with nasal injuries. Patients and Methods: this study was conducted at Al-Hussein Teaching Hospital/ Samawah city /Iraq during the period from January 2016 to December 2016. All patients with nasal trauma were included in this study. All patients were clinically examined by otolaryngologist. Conventional radiography and ultrasonography of the nose were performed for all patients and compared with the clinical diagnosis. Results: The sensitivity and specificity of ultrasonography and plain radiography in comparison with clinical diagnosis of nasal bone fracture was 97.8% and 85.7%, 88.6% and 62.5% respectively. Three cases were missed as nasal bone fractures on clinical examination at the time of the presentation while the results of ultrasonography remained similar whether it was taken during the time of the presentation or following the resolution of the nasal oedema. Conclusion: ultrasonography is more reliable than conventional radiography in the assessment of acute nasal injuries. Ultrasonography is not affected by the nasal oedema in the assessment of nasal bone fractures.
416 Determination the effect of ciprofloxacin and gentamicin alone and in combination to induce nephrotoxicity in experimental rats , Mays U. Hashim, Shaimaa H. Ali Al-Cekal, Ahlam J. H. AL-Khamas
This study aimed to evaluate the combination effects of ciprofloxacin (CP) and gentamicin (GT) in compare to their lonely effect to induced nephrotoxicity in experimental rats for (14) days administration. Doses of 100 mg/kg were used for each of CP and GT, and 50mg/kg each of CP and GT were combined to estimate the synergistic effect of two drugs. In this study we used 28 rats which were randomly divided into 4 groups (n= 7 in each group), grpup1: animals were given saline (NaCl; 0.9%) 1 ml/kg orally for 14days, group2 : CP alone at dose of 100 mg/kg orally for (14) consecutive days was given, group3: animals were given GT alone at dose of 100 mg/kg intraperitoneally (i/p) for (14) consecutive days, and the group 4: animals were treated with combination of GT (50mg/kg IP) and CP (50 mg/kg orally) for (14) consecutive days. After 2 weeks, the blood had been draw for biochemical evaluation and kidney sections had been take for histopathological examination. Nephrotoxicity was estimated by measuring biochemical markers: the serum creatinine (Cr), blood urea nitrogen (BUN) and Malondialdehyde (MDA) levels. The results of this study showed that there were significant (p< 0.05) or (p< 0.01) increase in Cr, BUN, MDA in combination of GT and CP groups than other groups. The administration of the combination GT with CP result in significant increase of nephrotoxicity in compare to administration of GT and CP alone respectively.
417 Determination the effect of ciprofloxacin and gentamicin alone and in combination to induce nephrotoxicity in experimental rats , Mays U. Hashim, Shaimaa H. Ali Al-Cekal, Ahlam J. H. AL-Khamas
This study aimed to evaluate the combination effects of ciprofloxacin (CP) and gentamicin (GT) in compare to their lonely effect to induced nephrotoxicity in experimental rats for (14) days administration. Doses of 100 mg/kg were used for each of CP and GT, and 50mg/kg each of CP and GT were combined to estimate the synergistic effect of two drugs. In this study we used 28 rats which were randomly divided into 4 groups (n= 7 in each group), grpup1: animals were given saline (NaCl; 0.9%) 1 ml/kg orally for 14days, group2 : CP alone at dose of 100 mg/kg orally for (14) consecutive days was given, group3: animals were given GT alone at dose of 100 mg/kg intraperitoneally (i/p) for (14) consecutive days, and the group 4: animals were treated with combination of GT (50mg/kg IP) and CP (50 mg/kg orally) for (14) consecutive days. After 2 weeks, the blood had been draw for biochemical evaluation and kidney sections had been take for histopathological examination. Nephrotoxicity was estimated by measuring biochemical markers: the serum creatinine (Cr), blood urea nitrogen (BUN) and Malondialdehyde (MDA) levels. The results of this study showed that there were significant (p< 0.05) or (p< 0.01) increase in Cr, BUN, MDA in combination of GT and CP groups than other groups. The administration of the combination GT with CP result in significant increase of nephrotoxicity in compare to administration of GT and CP alone respectively.
418 Effect of adding astaxanthin and taurine to the Tris extender on the semen quality of Awassi rams during cooling storage , H.J. Neamah, A.A.Houbi
This study was carried out at the animal farm\ Department of Animal Production\ Agricultural Engineering Sciences, College \ University of Baghdad, Iraq, during the period from 3\2 -28 \9\ 2019, to explore the effect of the adding astaxanthin and taurine to Tris extender on post-cooling semen characteristics of Awassi rams for different periods. In this experiment, used three mature Awassi rams. The semen was divided into nine experiments. Control group (without addition), T1 (1µМ astaxanthin), T2 (0.5 µМ astaxanthin), T3 (35 mM taurine), T4 (45 mM taurine), T5 (1µМ (astaxanthin and 35 mM taurine), T6 (1µМ astaxanthin and 45 mM taurine), T7 (0.5 µМ astaxanthin and 35 mM taurine) and T8 (0.5 µМ astaxanthin and 45 mM taurine), individual sperm motility, acrosome integrity, sperm abnormality and live sperm percentage were investigated following different periods. Using a combination of astaxanthin and taurine improved the individual sperm motility after dilution and after 72 hours. The addition of 45 µМ taurine has improved the percentage of acrosome integrity during different cooling periods. While the sperm abnormality was decreased after dilution and after 42 hours in group T6 and after 24, 48 in group T1. The results were showing that added astaxanthin and taurine separately or as a combination improve the live sperm percentage after dilution and after 24 hours. Through this study, we conclude the importance of adding astaxanthin and taurine in improving the qualities of diluted semen of Awassi rams.
419 The effect of ginseng plant on the activity of monoamino oxidase and peroxidase, Najlaa Qassim Muftin, Emad Mahmoud Eltayef , Muna Khalil Murtadha , Aliaa Hashim Farag, Shaemaa Hadi Abdulsada , Dhifaf Abdul Adheem Abdulabbas , Marwah Abbas Abed
Background: Ginseng is a medicinal herb that has been used in many laboratory experiments because of its pharmacological activities of its some constituents including ginsenosides, phytosterols, sesquiterpenes, flavonoids, polyacetylenes, alkaloids, and phenolic compounds. This herb has been used in many European and Middle Eastern countries as a traditional remedy for several diseases due to its antithrombotic, antioxidative, anti-inflammatory, and anticancer effects. So we designed this study to detect the effect of various concentrations of ginseng herp on the catalytic activity of monoaminoxidase (MAO) and peroxidase through in vivo and in vitro study. Methods: The in vivo study included the effect of Ginseng extract on the catalytic activity of MAO. Eighteen female mice were collected and distributed to three groups. The first group consist of six mice was not treated with ginseng extract (control group).Each of second and third groups consisted of six mice that were orally injected with 250 mg/kg and 450 mg/kg of body weight of ginseng extract respectively during fourteen consecutive days. The activity of MAO was calorimetrically measured at 272nm in the isolated brain mitochondrial fractions. The in vitro study of ginseng extract was calorimetrically tested in human serum of both MAO and peroxidase enzymes at 272nm, 510 nm respectively through two experiments; the first experiment includes measuring the activity of the enzymes using different concentrations of ginseng extract. The second experiment consisted of measuring the activity of the enzyme using different concentrations of substrate and constant concentration of ginseng extract. Results: Through in vivo and in vitro study, the outcomes manifested that ginseng extract is good inhibitor towards MAO and peroxidase enzymes. The results also revealed that the inhibition capacity of ginseng extract increased with growing its concentration. Where higher percentage of inhibition at highest concentration of ginseng extract (0.1 mg\mL) was 83.26% and 64.6% for MAO and peroxidase respectively. The inhibition Kinetic characteristics of ginseng extract were Vmax= 20 µmol\min\L,Ki=0.03 for MAO and Vamx =83.3 µmol\min\L , Ki= 0.003 (mol\L) for peroxidase, this results refer that ginseng extract is competitive inhibitor with MAO while is un competitive inhibitor with peroxidase. Conclusion: The results of this study revealed that the inhibitory capacity of ginseng extract towards both MAO and peroxidase. The inhibitory of properties of ginseng extract opens up new horizons towards the medicinal uses of this herb.
420 Preparation of nitrile derivative and study its effect as a possible novel drug for diabetes, Nadia Y Al-Tikrity, Firas SH- Abdulrazzaq, Ahmet Beyatli
The aim of the research is to synthesis one of the captopril derivatives by converting the carboxyl group to the nitrile group, and characterized by (FTIR, 1H-NMR, 13CNMR, Mass spectroscopy and CHNS). This process convert captopril structure design to a compound similar to the modern diabetes drugs (vildagliptin), with continuation of its effectiveness as an antihypertensive drug. After preparation of the modified drug, the effectiveness confirmed by some related enzymes, hormones, and some histological pictures of experiment animals (rabbits). The effects of the derivative on rabbits were studied. Sixty rabbits with weights (1500-1800)g, were divided into six groups (10 rabbits for each group). The first group, G1 consisting of 10 rabbits, is a healthy control group that has not been given any substance. The second group, G2 alloxan group (infected group). The third group gets the alloxan and vildagliptin. The fourth group gets the alloxan and the modified drug compared to vildagliptin dose. The fifth group gets the alloxan and the modified drug compared to captopril dose. The sixth group gets the alloxan and the captopril. After two weeks, the samples were withdrawn after 2 hours from the last dose, the serum was separated and the biochemical and enzymatic variables were studied including (glucose, insulin hormone, glucagon hormone, dipeptidyl peptidase-4, angiotensin converting enzyme, prostaglandin I2, alkaline phosphatase, aspartate amino transferase, alanine amino transferase.
421 The effectiveness of ethanolic extract of propolis on wound healing in albino rats, Ahmad Hassan Sahib , Maan Abdul Azeez Shafeeq , Salah Mahdi Mohsen
Background: Wound healing is a complex process. So, the use of natural products such as propolis, which has anti-inflammatory and antioxidant properties to accelerate wound healing, was an important target. Aim of work: The aim of this study was to assess the possible wound healing effect of ethanolic extract of propolis (EEP) on full-thickness wound model in albino rats. Materials and Methods: Healthy adult 72 albino rats were used in this study. Excision wound with 1.5 cm length was done on the posterior of the neck. Rats were randomly divided into equal four groups (negative control, positive control, ethanolic extract of propolis (EEP) ointment and tetracycline ointment 1%). Each group was divided into three subgroups that received treatment for 4, 7 and 14 days. Wound area in the experimental group was covered twice daily with a fixed amount of EEP ointment and tetracycline ointment (1%), the negative control group did not receive any treatment, while positive control group treated with physiological saline solution (0.9 % Na Cl). Histological analysis was performed and counting fibroblast, neutrophils, macrophages and new blood vessels in the wound bed was done followed by statistical analysis. Results: The findings show that healing process of excision wound in albino rats treated with the EEP ointment started earlier and had a faster course than the standard tetracycline therapy. Conclusion: The powerful effect of EEP ointment on accelerating wound healing was revealed. This could be an effective strategy for managing delayed wound healing.
422 Pregnancy diagnosis in Local Iraqi ewes: A Comparative study, T.R. Mohammed, A.F. Majeed, Ibtehaj1 K.H. Alawiy
This study was aimed to compare deferent methods of pregnancy diagnosis in local Iraqi ewes such as the level of progesterone and the ultrasonography and Compare it with laparotomy in order to know the accuracy of the methods of diagnosis. This study was conducted on private livestock farm in Heet city / Al-Anbar province far away about 180 km west of Baghdad. The number of animals were included in this study was 20 ewes of local Iraqi breed, Aged between 2-4years with a body weight between 40-50 Kg. The animals were oestrus Synchronized program. Blood samples were collected from jugular vein at 18 and 20 days of insemination, in order to measure the level of progesterone as first methods. Pregnancy was diagnosed with ultrasonography (second method) and third method for diagnosis through the laparotomy via left flank incision which is the judgment between the two methods. The result showed that the accuracy of pregnancy diagnosis method via level of progesterone, ultrasonography and the laparotomy were 88.5%, 92% and 100% respectively. There was a significant difference between the laparotomy method as Compared with ultrasonography and the progesterone level. It was concluded that the proper method of pregnancy diagnosis in ewes was the ultrasonography with allow risk.
423 Detection and evaluation of Streptococcus pyogenes (Group A) as a superior infectious agent of acute pharyngitis among school age children , Eman Abid Fahad Al-Hasnawi, Oruba K. Abbas, Wasan Al-Taie
Background: Streptococcus pyogenes Group A -(GAS), is a bacterium has gram-positive coccoid shaped, that appears in chains, and produces a small colonies with color like white to greyish, produces a clear zone of type β-hemolysis when cultivate within blood agar. GAS causes severe human disease mostly by three mechanisms include: the suppuration, as in cases pyoderma, and pharyngitis, immune-mediated inflammation and toxin elaboration. Causes respiratory tract infections, with acute pharyngitis, are common through childhood and adolescence. And that occur most commonly and frequently among the children between (5 and 15) years of age. Objective: This study for searching about infection of Iraqi children at school age with Streptococcal pharyngitis by Streptococcus pyogenes Group A (GAS). Patients and methods: Collection of (60) samples from school children at age between (6- 12) years suffered from acute pharyngitis, from many schools in different regions in Baghdad city, with (20) healthy children for control. from period January to April 2019. Two swabs were collected from each child, Swabbing the posterior pharynx and tonsils, for both the rapid antigen-detection test and culture, with dimension of serum antibodies to Streptolysin-O. Results: Results of Culture on Blood Agar for genus Streptococcus were in 32 (53%) patients were mostly in age group (6-8) years: 23(38%), was in males (10), in female (13), with total Medical history of infection (14) while in control total (4). Also specific result by rapid antigen-detection test, Streptococcus pyogenes detection were in: 25 (42%), mostly in age group (6-8) years, was 19 (32%), in male (8) and in female (11) with total Medical history of infection (7) while in control were (1) in total. Conclusion: In school age patients with signs and symptoms, revealing of streptococcal pharyngitis, a specific diagnosis should be determined by substitute a throat culture, or a rapid antigen-detection test with execution a throat culture, if the rapid antigen-detection test be negative, as a minimum in children in school age. But if the rapid antigen-detection test was positive, a throat culture will not desirable for diagnosis, nor is necessary after treatment, if symptoms were resolved.
424 Use of Robson Classification to Assess Cesarean Section Rate in Al-Samawah City: The Role of Source of Payment for Childbirth, Dr. Enas Yassen Jasim, Sama Hisham Al-Gharah
Background: Cesarean section (CS) rates are increasing worldwide but there is some concern with this trend because of potential maternal and perinatal risks. The Robson classification is the standard method to monitor and compare CS rates. Our objective was to analyze CS rates in Al-Samawah city according to source of payment for childbirth (public or private) using the Robson classification. Patients and Methods: This data was collected from Al-Samawah Feminine and children teaching hospital for a period of 1 month from 8/August/2018 to 7/September/2018. We categorized all women into Robson groups and reported the relative size of each Robson group, the CS rate in each group and the absolute and relative contributions made by each to the overall CS rate. Results: The overall CS was 38.8% in which CS deliveries in public sector is more than private sector. The Robson groups with the highest impact on Al-Samawah CS rate in both public and private sectors were group 5( Previous CS, single cephalic, > = 37 weeks) . High-risk women had significantly greater CS rates compared with low-risk women in almost all Robson groups. Conclusion: Public policies should be directed at reducing CS in nulliparous women, particularly by reducing the number of elective CS in these women, and encouraging vaginal birth after cesarean to reduce repeat CS in multiparous women
425 THE PROTECTIVE EFFECTS OF EXTRACT OF PUNICA GRANATUM L PEEL AND PIPER LONGUM FRUIT AGAINST HEPATOTOXICITY INDUCED BY THIOACETAMIDE IN MALE ALBINO RATS, JAFAR, S. N, MAWLOOD, K. A
The purpose of this study was to evaluate the protective effects of the extract of Punica granatum L peel (PPE) and Piper longum (PL) fruit, against thioacetamide (TAA)-induced liver toxicity in male rats. Experimental animals were divided into six groups, control, TAA, PPE200, PPE400, PL200, PL400. TAA administrated intraperitoneally, and treatment with herbal supplements was orally given for 14 weeks. Liver tissue was used for extraction and evaluating of the TNF-α gene expression and the homogenized liver tissue used to evaluate malondialdehyde (MDA) and antioxidant enzyme activities; superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GSH). Blood sera were used to measure liver enzymes. The results indicated that TAA (200 mg/kg BW) could significantly decrease SOD, CAT, and GSH whereas increased AST, ALT, ALP, and MDA (p
426 Synthesis and characterization of n-(4 sulfamoylphenyl) benzamide derivatives, R. Udhayasurian, K. Sivakumar, A. S. Suman Sankar, S.T. Gopukumar
A series of molecules containing sulfonyl and amide coupling structure were developed, synthesized and characterized. Sulfonamides are a very important class of compounds in the pharmaceutical industry. In this work, a new class of sulfonamide derivatives is being synthesized from 4-aminobenzene-1-sulfonamide with benzoic acid and its derivatives (1a-ah) using peptide coupling reagent EDCI and HOBt with different melting point. The progress of reaction was monitored by the use of thin layer chromatography and the structures of the synthesized compounds were elucidated and confirmed by FT-IR, 1H NMR and 13C -NMR spectroscopic analysis.
427 Synthesis and characterization of n-(4 sulfamoylphenyl) benzamide derivatives, R. Udhayasurian, K. Sivakumar, A. S. Suman Sankar, S.T. Gopukumar
A series of molecules containing sulfonyl and amide coupling structure were developed, synthesized and characterized. Sulfonamides are a very important class of compounds in the pharmaceutical industry. In this work, a new class of sulfonamide derivatives is being synthesized from 4-aminobenzene-1-sulfonamide with benzoic acid and its derivatives (1a-ah) using peptide coupling reagent EDCI and HOBt with different melting point. The progress of reaction was monitored by the use of thin layer chromatography and the structures of the synthesized compounds were elucidated and confirmed by FT-IR, 1H NMR and 13C -NMR spectroscopic analysis.
428 Eco Friendly and Bio Safe Copper Nanoparticles Synthesis in Liv-Pro-08 and Its Antimicrobial Activity, Anandhi Eswaran, Suriyavathana Muthukrishnan, Kavitha Rani Mari ,Punithavathi Manogaran, Manikandan Madhaiyan
Nanoparticle research is a captivating branch of science can be traced back to ancient times. Researchers have always been paying attention in synthesizing nanoparticles using modern day technologies but their existence in nature is very evident. The green method of biological Nps synthesis is challenging approach which renders preparation in aqueous conditions with low-costs and low energy requirements. The goal of this study deals with eco-friendly and bio safe copper nanoparticles developed from aqueous extract (Liv-Pro-08), characterization by using different Spectroscopies and Microscopic methods to evaluate their antibacterial activity and antifungal activities by Cup diffusion method and Pour plate method. The spectroscopy results perfectly denotes that Liv—pro-08 having the functional groups responsible for green synthesis. SEM images observed spherical shape compounds. Assessment of an anti-bacterial effect against treated microorganisms (P. aeroginosa, E.coli, K. pneumonia) and their antifungal capacity showed synthesized Nps shows great capability to oppose Aspergillus. Niger and F. oxysporum compared to standard antibiotic tetracycline and quardiline respectively. The final findings shows the developed cuNPs express valuable anti-bacterial properties.
429 Studies on Antimicrobial Activity of Syzygium Cumini and Syzygium Alternifolium, Vipin Kumar Sharma, Chitra D, M. Charumathy, Lekshmi Gangadhar, Anooj.E.S
Background: Antibiotic defiance in bacterial is a satisfactory obstacle in the latest world. Day-to-day novel dopes are being intact to defeat this drawback. The goal of this is trained used to be to check antimicrobial resistance of the Syzygium cumini Syzygium Alternifolium fruit . The dried fruit of Syzygium Cumini Syzygium Alternifolium fruits are located in a southern state of India. Method: The extract of dried fruit and seed of Syzygium Cumini Syzygium Alternifolium was once ready through using specific solvent of Ethyl Acetate. These ready extracts have been used for evaluation of antibacterial ability in opposition to three Gram-positive bacterial stains (Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa) and two gram-negative bacterial stains (Streptococcus, Staphylococcus aureus). Results: The antimicrobial ability was appraised by Disc diffusion assay of Syzygium Cumini Syzygium Alternifolium. The zone of inhibition used to be determined at the concentration of (500µg/ml) in G.Gummi-gutta Streptococcus& Pseudomonas aeruginosa (14mm), Staphylococcus aureus& Salmonella typhi (9mm), Escherichia coli (8mm). The zone of inhibition was once determined at the concentration of (500µg/ml) in G.Sylvestre.The Streptococcus(11mm), Staphylococcus aureus& Escherichia coli (9mm), Salmonella typhi(10mm), Pseudomonas aeruginosa (8mm) respectively. Conclusion: Due to the fact, the extract confirmed a wide spectrum of the antimicrobial undertaking of each Gram positive and gram negative bacterial isolates. Comparative reports of the. Syzygium Cumini Syzygium Alternifolium fruit and seed. The nice one among Syzygium Alternifolium. Can be utilized as a bio preservative or therapeutic agent to prevent a few illnesses. The dried fruit of Syzygium Alternifolium would characterize a novel antimicrobial obtain with steady, adoptive energetic compounds that may prove a scientific base for the consumer in recent treatment.
430 Purification and Characterization of Hemolymph Lectin of Freshwater Crab, Lamella Lamellifrons (Alcock, 1909), S. Mary Mettilda Bai1, M.R. Basil Rose
Lectins offer novel biomaterials useful in therapeutics and understanding animal lectins is of high value in the field of protein chemistry and designing new drugs on the principle of protein-carbohydrate or protein-protein interaction. The objective of this study was to isolate and characterise a brachyuran crab lectin. A 68 kDa O-acetyl sialic acid specific lectin, lamellin was purified from the hemolymph of the freshwater crab, Lamella lamellifrons using single step affinity chromatography. Lamellin strongly agglutinated rabbit and buffalo erythrocytes and was inhibited by BSM followed by bovine and porcine thyroglobulin. The optimum activity of the lectin was achieved between pH 7-7.5 and temperature 30-40oC. The purified lectin was calcium dependent, sensitive to EDTA and had marked specificity to the O-acetyl group of sialic acid which was validated with a range of assays such as hemagglutination (HA), hemagglutination inhibition (HAI), de-O-acetylation and neuraminidase treatment of the glycoprotein inhibitors. Purification of a sialic acid-specific lectin with a strong affinity for O-acetyl group will find immense application in recognising pathogens and tumor cells.
431 Assessment of Breast Cancer Treatment management in Karbala City (A Descriptive Study) , Abdulrazzaq Kalaf Hassan
Background: breast conserving surgery provide good choice for treatment of stage 1 and II breast cancer and has equivalent out come in term of survival and prognosis. Aims of the Study: To evaluate breast cancer treatment according to multiple variables and patient's wish in Al-Hussain hospital in Karbala city. Patient and methods: - The present study is considered as a descriptive study, which has been conducted at AI-Imam AI-Hussein medical city in Karbala governorate during the period from 1st September 2017 to the 30 of December 2018. Fifty-two patients included in the study were admitted to the general surgical ward. The entire patients were first visit the breast clinic or general surgical out-patient clinic or private clinic, every case is assessed by triple assessment (history and clinical examination, imaging study, histological diagnosis). Each case was presented and discussed in MDT (multidisciplinary team), after that we do the operation (wide local excision) and the specimen without formalin send for the lab unit in our hospital for diagnosis and assessment of the margins, the result usually come after 20-30 minutes. As the result come back, if the margins were free then we start with axillary dissection or we do re-excision if the margins were positive and then the wound closed with closed system drain after secure hemostasis, and the axillary dissection is standard to the level II L.N dissection. Results: Fifty-two patients with breast cancer included in our study, twenty three patients (44%) were treated by mastectomy and twenty nine patients (56%) were treated by breast conserving surgery. The most common type was invasive ductal carcinoma, which can be treated by either modality (breast-conserving surgery or mastectomy). A sentinel lymph node biopsy (S.L.N.B) is important to avoid negative axillary dissection. In conclusions; screening program for breast cancer to detect early disease is very important subject before proceeding to axillary dissection S.L.N.B is very important and useful. The choice of Mastectomy was lower than that of Breast Conserving Surgery in our study for early treatment with breast cancer.
432 Evaluation of the effect of different light cure devices/modes on the micro-leakage of class V composite restoration, Hamsa Zaki Al-Assadi, Thanaa GhaniNema , Ala' Mahdi Muhamedali
Introduction: Polymerization shrinkage has remained the major problem of composite restorations.This shrinkage can lead to micro-leakage between the composite material and tooth structure that adversely affect the longevity of the restoration. Many improvements of materials and curing devices had occurred to overcome these drawbacks. This study aimed to assess and compare the effects of different light cure devices/modes on the leakage of class V compositerestoration. Materials and Methods: A total of 64 standardized cavities (Cl V) were prepared on the buccal and palatal surfaces of 32 recently extracted human premolars. Teeth were divided randomly into 4 groups (N=8) according to the type of light curing unit used. All cavities were restored using flowable composite resin (3M ESPE) shade A2, and then they were finished and polished according to manufacturer's instructions. The specimens were subjected to thermocycling and dye immersion in 2% methylene blue. Then all teeth were sectioned longitudinally and examined for dye leakage scores under stereomicroscope with (40 X) magnification. Results: Using one- way ANOVA analysis and Student’s t-test.The results of this study revealed that there were statistically significant differences (p
433 Adverse Outcome of the First Trimester Gestational Age Discrepancy: A Cohort study, Abdul Razzaq Oleiwi Jasim, Salam Geddoa, Fatima Aljassany
Background Ultrasonography is considered the most accurate method to estimate the gestational age (GA) comparing to the GA based on the last menstrual period (LMP) and or clinical examination. Aim of the study To determine the association between the spontaneous abortion and discrepancy of the gestational age. Method A cohort study was conducted among 459 pregnant woman aged 17-43 years with gestational age ranged between 7-13 weeks measured according to the last menstrual period (LMP), 27 women were missed transvaginal ultrasonography was done for all patients at the first time. The gestational age was calculated according to the last menstrual period (LMP), crown–rump lengths (CRL), mean gestational sac diameters (MGSD). We divided the sample into two groups, the study group which had gestational age discrepancy and the control group without discrepancy, then we followed the women for the 20th week of pregnancy and compared the spontaneous abortion rate between the two groups. Results 27% of the sample had gestational age discrepancy more than one week , 28.7% of the study group versus 4.1% of control group respectively developed 1st trimester abortion, and there was a significant association between the increase risk of spontaneous abortion and gestational age according to mean gestational sac diameters MGSD, crown–rump lengths (CRL). On applying binary logistic regression analysis, History of previous abortion (OR = 3.122, P = 0.002) ,History of chronic diseases (OR = 1.415, P = 0.004 ) and Yolk sac abnormality (OR = 11.601 , P = 0.000 ) were the sole factors significantly associated with increased odds of spontaneous abortion among the women . Conclusion Discrepancy of the gestational age and abnormal sonographic appearances of the yolk sac increases significantly the risk of spontaneous abortion among the women in the sample.
434 The Effect of Essential Oils on Microbial Biofilm on Denture Base Surface. , Makarem M. Abdulkareem
Nowadays, there is a globally effort to find solutions to replace the synthetic drugs with the therapeutic power of natural products to reduce the percentage of many side effects resulted from conventional treatment. The main objective of this research is to investigate the antimicrobial action of essential oils against grown microorganisms on the surface of acrylic resin denture base materials. In terms of methodology, four types of natural oils (Linseed, Harmal, Radish and Black seed) have been used to investigate their antimicrobial effects against the biofilm formation of three types of bacteria and one fungus on the surface of denture base material. Totally, 30 specimens (10mm×10mm×2mm) prepared from the heat cured acrylic resin denture base materials are provided. One-way ANOVA has been utilized in data analysis at P ≤ 0.05 or 0.01, also t- test is used for independent samples. According to the results, all the concentrations of radish oil and black seed oil have shown a significant effect on bacteria. Regarding its effect on fungus, only radish oil has shown a significant effect against Candida albicans. Linseed oil is less effective than other types of essential oils used in this study particularly against Streptococcus pyogenesa. As a result, all the tested natural oils have shown antibacterial effect, while only radish oil has shown significant effect against candida albicans.
435 An In vitro study to evaluate the penetration of various sealant materials with different conditioning techniques, Ali Mohammed Hussein, Ali Fadhil Hashim
Fissure sealant and pit are taken a great adjunct to oral health care to decrease the low progression and occlusal caries onset. The objective of this article is for evaluation the effect of various fissure sealant and conditioning methods on penetration of sealants.36human mandibular first premolar and sound maxillary teeth have been gathered and extracted for orthodontic aim among teenagers from 15 to 20 years old. Teeth were incubated for 1 day and randomly grouped in two similar set. Each set includes 18 teeth with the same mandibular and maxillary teeth number. One group (set)has been treated with bonding agent, whilst another group has been treated without bonding agent. Each set has been divided into 3subsetsbased on 3various sealant used material(s)as 1)Tg (without fluoride) sealant, 2)Tetric N-flow (flowable composite) and 3) SDI (containing fluoride) sealant. Each sub-group includes 3 maxillary first premolar and 3 mandibular (totally 6 teeth). Later, the crowns of teeth’s crowns have been longitudinally partitioned in a buccolingual direction by an imaginary line between two points that was previously spotted. All sections were examined under electronic microscope and took image. Also, a ranked scale technique has been applied to measure penetration. The results have shown that group A+ and C+ have more penetration (score 4), whereas those groups with fluoride B and B+ have shown lower penetration of sealant materials. As a result, after etching enamel surfaces and before using sealant materials, the use of bonding agent could facilitate and increase the penetration of sealants, which increases the retention of sealant materials to enamel surfaces.
436 The Effect of Training on Sand on Muscular Power, Legs Tolerance, Various Defensive Movements, and Lactic Acid of Handball Players, Hikmat Al-Lime
The current study aims to develop muscular power and legs tolerance, defensive movements and lactic acid of handball players through using training on sand as a type of resistance training to reach the development of lactic acid that serve these abilities and develop their performance of various defensive movements of handball players. The researchers see that training on sand is one of the easy methods of training that prepares and restores the body ability of direct control help to raise the ability of the muscles on their full stretch in addition to enhance player's efficiency physiologically. In order to achieve the study objective, Al-saniyah handball club adult (18) players are selected. The players' heights are (181.2±5.674 cm), weights (75±4.872 kg) and ages (22.3±3.982 years). The players are divided into two groups, (9) players for each group. The first experimental group is given special training exercises on sand and prepared by researchers for (8) weeks and (3 units) per week with an intervention in the main part of the training unit. The second control group adheres to the training course prepared by the coach of the club. After the series of measures, it is concluded that the exercise prepared by the researchers can be adopted to develop the muscular power, legs tolerance and defensive movements in addition to lactic acid for handball players.
437 The Effect of Training on Sand on Muscular Power, Legs Tolerance, Various Defensive Movements, and Lactic Acid of Handball Players, Hikmat Al-Lime
The current study aims to develop muscular power and legs tolerance, defensive movements and lactic acid of handball players through using training on sand as a type of resistance training to reach the development of lactic acid that serve these abilities and develop their performance of various defensive movements of handball players. The researchers see that training on sand is one of the easy methods of training that prepares and restores the body ability of direct control help to raise the ability of the muscles on their full stretch in addition to enhance player's efficiency physiologically. In order to achieve the study objective, Al-saniyah handball club adult (18) players are selected. The players' heights are (181.2±5.674 cm), weights (75±4.872 kg) and ages (22.3±3.982 years). The players are divided into two groups, (9) players for each group. The first experimental group is given special training exercises on sand and prepared by researchers for (8) weeks and (3 units) per week with an intervention in the main part of the training unit. The second control group adheres to the training course prepared by the coach of the club. After the series of measures, it is concluded that the exercise prepared by the researchers can be adopted to develop the muscular power, legs tolerance and defensive movements in addition to lactic acid for handball players.
438 Topographical Patterns of Keratoconus in Iraq, Alyaa Abdulameer Kadhum, Haider Aswad layikh, Hasanain Hasan Attar
Purpose:The purpose of our study is to view the curve-distribution of variable topographical patterns among Iraqi people and to compare the data with other Asian results. Methods:This "cross sectional" study included patients all of them presented to Al_Diwanyia Teaching Hospital in Iraq, asking for visual correction. Period of data collection from May 2018 to September 2019.All participating subjects were diagnosed with the disease in both eyes and did not do any surgery for their keratoconus at the time of study- data collection. Results: This study includes 94 eyes of47 patient from Al_Diwanyia city; all examined by pentacamtopographic system diagnosed with keratoconus bilaterally, the most common pattern was asymmetric bowtie with angulation followed by asymmetric bowtie inferior steep then the round and irregular shapes respectively. Conclusion: Asymmetric Bowtie with angulation (A.B. with angulation) was the most Common topographic type, followed by Asymmetric Bowtie Inferior steep(A.B.I.S.), Round and Irregular The patterns are similar to that of other Asian people.
439 A retrospective Study of Colorectal Carcinoma in Kirkuk, Iraq, Chalack Qader Nader, Naisan Khaleel Abdullah,Muna Adil Mohammed
Colorectal cancer (CRC) is a common disease with global burden as the 4th frequently diagnosed malignancy in male and female; approximately withone million sample developing CRC per year. It is the 3rd death cause of cancer in worldwide [1]. This colorectal carcinoma research could assist us to diagnose the clinical symptoms of disease, histopathological typing and grading for identifying the surgery process beside other therapeutic options as well as disease outcome(s).In terms of methodology, this study as a retrospective research includes 140 colorectal carcinoma analyzingcases, clinic-pathological characteristics and results after various therapies asradiotherapy, surgery and chemotherapy. As a result, it is found that colorectal carcinoma is highly seenin 40 to 60 years of age with male predominance. Also, lymph node metastasis is more common than other sites’ metastasis. The diagnosing of lymphatic metastasis and infiltration of surrounding tissues are more accurately performed through CT scan.Therefore, in this study, the sphincter saving process percentages were low in rectal malignancies. Additionally, all the database respected gender - age incidences, clinic-pathological characteristics and therapeutic resultscould be compared to the related literatures.
440 Molecular Detection of Chlamydia trachomatis from sexual active women suffering from pelvic inflammatory disease, Hadaf Mahdi Kadhim
One hundred and twenty attendees at the STD clinic in Al-Diwaniyah children and maternity hospital were examined for the presence of Chlamydia trachomatis in women who are suffering from pelvic inflammatory disease, in this study, the molecular assay that used for chlamydia detection was PCR. Overall, 29 (24.1%) of 120 women examined had Chlamydia trachomatisin highly vaginal swabs,10, 15 and 4 of Chlamydia trachomatisbacteria were identified by polymerase chain reaction assay in women have aged as 18-35 years, 36-55 years and older than 55 years, with a prevalence rate of 8.3%, 12.5% and 3.3%, respectively. The current study not found any significant association (X2(2)=3.29, P>0.05) between aged and number of Chlamydia trachomatis, also the kruswallwallis test showed that no statistically significant differences in women age and the different sexual intercourse per week (X2(2)=50.04, P
441 Epidemiological study of Splenic Injury cases in Baghdad Province , Nissan Khaleel Abdullah Al.Azzawi,Salah Salih Mohammed, Bashar Akram Abed
This is a prospective study of splenic trauma managed at AlYarmouk teaching hospital. The data of the 82 patients were analyzed during the period of study between May 2003 to December 2004, it was found that the highest incidence was encountered in those between 20 and 30 years (42.2%) and 76 of cases (92%) were males. Penetrating injuries were more common than blunt injuries (62.1% versus 37.9%). Abdominal tenderness and rigidity were the commonest clinical presentation The majority of patients (90.2%) were diagnosed at laparotomy. Splenic injuries were graded in severity from I-V, the highest percent (35.3%) was found to be grade HI splenic injury. Splenectomy was the treatment in the majority of the patients (92.6%), and in two of them, auto-transplantation was performed. Associated injuries were frequently occurring in (69.5%) of patients and the most common one was liver injury (43.8%). Atelectasis and other pulmonary complications had the highest incidence of postoperative complications. The overall mortality was (11%).
442 Pathological potential effects of Alpha-Lipoic acid in preventing cataract Diabetic retinopathy in albino male mice, Bassim M. Jwad , Anas. A. Humadi, Bushra I. AL-Kaisei
Alpha-lipoic acid (α-LA) is prevention complication of diabetes mellitus its potent antioxidant by prevention diabetic retinopathy, 30 albino male mice randomly equally divided into 3 groups 1st group (control) , 2nd group daily injected for 7 days I/P (100)mg/kg streptozotocin, 3rd group daily injected for 7 days I/P (100) mg/kg streptozotocin with α-LA (400)mg/kg for 90 days, the fasting glucose concentration showed increased in 2nd group in 30 & 90 days, while the fasting insulin concentration and glutathione concentration showed decreased in 2nd group in 30 & 90 days, the most pathological lesions showed in 2nd group in 90 days included cataract, disappear and atrophy of retina layer, necrosis, increased eosinophilia lens epithelium and finally multiple atheromatous artery.
443 Molecular Detection of High-Risk Human Papillomavirus Genotypes from Cervical Lesions in Baghdad, Sadeq K. Hachim , Aesha S. Ali , Maysaa Kadhim Al-Malkey
Objective: Using two complementary techniques of virus detection human papillomavirus (HPV) [capture of hybrids (CH) and polymerase chain reaction (PCR)], relate the cytological study and/or cervical biopsy with high-risk HPV (HPV-HR) genotypes presence, as well as relating their viral load (VL). Methods: About 272 women, who presented most cell alterations compatible with lesions cervical HPV, which has been detected in all high risk by the CH method and HPV genotype detection by PCR. Results: In 22% of the patients it was not detected HPV DNA. Genotype 16 and/or 18 was prevalent and was found in 33% of the 212 women studied, meanwhile, mixed infections were found by several genotypes in 25%. In as for the histological lesions found, in 61 patients with squamous intraepithelial lesions of high grade (H-SIL) and cancer, 55.73% presented genotypes 16 and/or 18, while in 38 patients with presence of altered squamous cells of significance uncertain (ASCUS) and 126 with squamous lesions low-grade intraepithelial (L-SIL), were put on manifest these genotypes only in 7.9% and 22.2%, respectively (p
444 The utility of platelet indices in diagnosis of urinary tract infection, Khalid KhalilAlaaraji, Qahtan M. Al-Obaidy, Siraj Shamsi Mahdi
Background: Urinary tract infection (UTI) is one of the most common infections in children and may lead to serious morbidity (such as chronic pyelonephritis and renal scarring) and mortality. Thus, early, appropriate, reliable diagnosis in children is mandatory and prompt antimicrobial treatment is required to minimize renal scarring and progressive kidney damage. When the diagnosis is underestimation may lead toshort-term or long-term complication while when the diagnosis is overestimation may lead to use over and unnecessary investigation and work up and invasive proceduresfor diagnosis even in healthy children. Aim of the study: 1. The platelet indices could be used as an index for immediate diagnosis and management of UTI even the results of urine culture is not available. 2. What is the relation between the changes in platelet indices with the type of micro-organism (gram +ve or gram -ve) for proper choice of antibacterial therapy? Methods: This study (prospective case–control)is done in Karbala teaching hospital for children, holly Karbala, Iraq. In the period from1, March to 31, December 2018.The study population consist of 105 children with culture proved UTI and 105 non UTI cases as a control,their age ranging from fifteen days to fourteen years’ old. Children with hematological and non-hematological diseases affecting platelet indices were excluded. Platelet indices were taken including platelet count, mean platelet volume (MPV) and platelet distribution width (PDW) which statistically analyzed. Results: A total of 105 children with culture proved UTI, 53 (50.5%) female and 52 (49.5%) male and 105 non UTI cases as control, 58 (55.2%) female and 47 (44.8%) male were enrolled in this study with no significant differences between both groups in demographic variables. Regarding the results of culture, 64 (61%) patients had gram negative rods while the remaining 41 (39%) patients had gram positive cocci and the main causative agent was E. Coli in 49.5% of total cases.The comparison of hematological parameters, revealed that mean hemoglobin level was significantly lower than that of controls, while platelet count, MPV, PDW, white blood cells and (WBC), were significantly higher in UTI cases than controls, in all comparisons, P
445 Screening of Amebic dysentery in Al -Noor Pediatric Hospital by a Contemporary gene, Saba Fadhil Ali Malaa, Dhamiaa Maki Hamza, Khalid Khalil Alaaraji
Abstract: Amoebic dysentery caused by parasitic protozoanEntamoebahistolytica . This disease transmitted in areas that allow poor sanitation and food with fecal contamination. The absence of delicate techniques is a significant issue connected with identification for this parasite. polymerase chain reaction (PCR) is efficient in addressing many of the constraints Because PCR's sensitivity depends on the validation of parasitic DNA extractions. The perfect diagnosis system based on morphologic characteristics of the cysts and trophozoites is not efficient molecular methods can deliver genotypic characterization complete. The E current study aimed to investigate the E.histolytica in infant's diarrhea depending on unusual gene (CL6EHI-188180). The stool samples were collected from infected children and first diagnosed microscopically and then diagnosed by PCR techniques, DNA was extracted, the CL6EHI_188180 gene used as the target for PCR amplification which rarely used. The samples collected from a total of 170-child aged from one month to two years old attending the hospital and the private laboratories in Al Hila/ Iraq during the period from October 2018 to June 2019, the samples examined by direct microscope and used of Iodine staining to make the internal structures more clearly. The outcomes of the study showed that the rate of infection overall was 35 (20.58%), the highest prevalence in the age group from six months to one-year-old was (11.17 %). The study indicated also that the ratio of infected among males (11.17%) are higher than females (9.41%). In addition, the percentage of infection among children living in the rural population was 12.35%, Also the percentage of infection among children feeding on bottle-feeding was 36.08%. The proportion of infection with the amoeba parasite was 9.41% from 16 positive samples when using the PCR technique by using a new gene as a marker.
446 Molecular Epidemiology of Hepatitis C Virus Infection Among Chronic Liver Disease Patients in Tamilnadu, India., S.Magesh, K.Kaveri, P.Gunasekaran, B.V.Sureshbabu, Dr.K.Sathiyamurthy
Background: Hepatitis C infection is the global health problem in which growing burden of health caring and our society. Among all hepatitis C virus (HCV) infected chronic liver disease patients, subtype one and three were the most common genotype in south India. This study evaluates the molecular epidemiology and epidemic nature of HCV genotyping in Tamilnadu. Methods: Two thousand one hundred and eighty eight serum samples were collected from suspected chronic liver disease patients from government hospitals situated in Chennai region. The samples were tested for HCV IgG ELISA, then positive samples were analysed by RT-PCR and sequencing was done for the identification of the HCV genotype in Chennai, Tamilnadu by Phylogenetic analysis. The data attained in this study were statistically examined by the software SPSS.20 IBM version. Results: Out of 2188 samples tested, 170 samples were HCV IgG ELISA positive in which 41 samples were positive for HCV RNA by RT-PCR. Among 41 HCV RNA positive cases, 9 (21.95%) were genotype 1a, 21 (51.22%) were genotype 1b and 10 (24.39%) belonged to genotype 3a. The remaining one (2.44%) sample was untypeable by RT-PCR. Conclusion: The estimated history of HCV infection in Tamilnadu may help to predict the future burden of HCV related diseases and assist better public health control and surveillance.
447 Isolation, Identification and Screening of Exopolysaccharides from Marine Bacteria, P. Ramya, D. Sangeetha, Anooj E.S, Lekshmi Gangadhar
Exo-polysaccharides are polymers of carbohydrates secreted outside their cell walls by certain fungi and bacteria. Marine microorganisms have considerable osmotic resistance and cause exo polysaccharides to be produced also in salt concentrations. The bacterial exo polysaccharide was isolated from the marine ecosystem. Samples of marine soil and sea water are gathered from Parangipettai, Pichavaram, Cuddalore, Poombuhar, Nagapattinam coastal areas in Tamilnadu, South India. In the present study, marine bacteria were isolated and screened for the production of exopolysaccharides using different screening methods viz., Congo red agar plate, String test, Congo red-Trypan Blue Assay method. Thus this study demonstrated that the eight marine bacterial isolates may prove to be potential strain for commercial production of exopolysaccharides.
448 Dyslipidemia in patients with hepatitis C virus infection, Laith Aldabbagh , Dr. Abdulhamza Rajooj Hmood , Ahmed Khalid Faleeh Aldahalemi , Sadiq Almuhana , Ali Sadiq Almussawi
Background: Hepatitis C is a blood-borne viral illness responsible for significant morbidity and mortality all over the world. Hepatitis C virus (HCV) interacts with the lipid receptors and is secreted as very low density lipoprotein (VLDL).It enters the hepatocytes, circulating as lipoviroparticles (LVP) So ,HCV may leads to disturbance of serum lipid levels. Aims of the Study: We aim to investigate the effect of HCV on lipid profile of patients with HCV infection and degree of dyslipidemia in relation to viral load, disease duration, and viral genotype Patients and methods: Total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL) , and low density lipoprotein (LDL) were measured in (100)patients with hepatitis C infection and (100) normal persons. Results: The mean total cholesterol(TC) level was lower in HCV cases than non-infected persons; (174.8 ± 37.9) mg/dL vs. (189.3 ± 21.4) mg/dL, respectively, HDL-C (42.3 ± 9.5 vs 52.8 ± 11.8) respectively, VLDL (27.2±6.6 vs 32.6±12.8)respectively, LDL (106.3±35.4 vs110.3±25.2) triglycerides (132.7 ±34.2 vs 163.1±31.6) respectively. HCV cases were more likely to have hypolipidemia (TC< 150 mg/dL) than non-infected persons; 19% and 6%, respectively, while normal levels and hyperlipidemia were more frequent among non-infected persons. No statistically significant correlation had been found between lipid profile and duration of HCV or viral load. No statistically significant differences in all lipid profile except HDL-C levels(Fig. 3.5) between both genotypes subgroups Conclusions: Hepatitis C virus affect lipid levels and can cause low TC, low HDL, low LDL, low TG. and lipid profile is not significantly affected by viral load or disease duration.
449 , Mufdlilah, Rolita Efriani, Dewi Rokhanawati, Nur Rachman Dzakiyullah
Background: the cases on diabetes melitus rise throughout a year, as well as diabetes melitus during pregnancy. Diabetes melitus during pregnancy has serious impact on mother and baby, if it is not handled optimally. Gestational diabetes mellitus (GDM) prevelancy reaches 9,3-25,5% all over the world. The numbers of diabetes mellitus patients during pregnancy in Indonesia approximately 1,9-3,6%. This research purposed to find out the mother’s obstacles in maintaining GDM. Methods: This research is a qualitative study with phenomenology approach. It was conducted from October 2018 to June 2019. Interviews method was performed to collect the data and supported with documentation study over 8 mothers who have history of gestational diabetes mellitus. Thematic analysis is used for data analysis. Results: The qualitative analysis result reveals two themes, which are reaction towards GDM diagnose and GDM management problems. GDM management problem covers the difficulties during pregnancy and GDM counceling. The result of the research indicated that mothers have difficulties in controlling eating pattern and adapt with GDM, as well as the limitation of information and communication that received by mother regarding to pregnancy with DM. Conclusion: This research reveals mother’s experience on obstacles in obtaining GDM services. The results could be useful guide to develop comprehensive self-care service, by focusing mother’s self-management and the role of health-workers. The urge of more effective GDM counselling is required to ensure clear understanding about principles of self-management on pregnancy mother, to assist mother in enhancing adaptation and ability in handling GDM.
450 Serum CA-125 for early prediction of miscarriage, Aseel Abdulameer Mohammed, Zainab Abdulkadhim Ftnan, AfrahMakki Mohammed
Background: Miscarriage is defined as the loss of a pregnancy before 20 weeks of gestation or before the fetus reaches 500 g of weight. It is estimated that 30% of all pregnancies end in abortion. 20% occurs in subclinical form (before evidencing it with ultrasound) and 10% later of his clinical detection. Objective: To assess the level of Serum CA-125 inpregnant with threatened miscarriage. Patients and method:A case control study carried at Al-Zahra Teaching Hospital in the period from 1st of October 2018 to the 1st of April2019, in which167 pregnant respondents at 6-12 weeks of gestation (were calculated from last menstrual period), with regular cycles) were enrolled in the study, and divided into two groups: Case group (n=58) pregnant with threatened abortion and control group (n=109) normal pregnant women. Results: the mean serum level of CA-125 in case group (threatened miscarriage) was (51.2±22.9 IU/ml) and for control group was (22.3±6.7 IU/ml). It was found that the serum level of CA-125 were highly significantly increased in threatened miscarriage group than in normal healthy group (P
451 Study on Self-esteem and Resilience among Locomotor Disabled Persons in Kashmir Division, Pankaj Singh, Mudasir Ahmad Wani
Background: Disability is a serious concern now a days and it has increased rapidly because of numerous reasons. Physical impairments and restrictions in daily activities may be the cause of isolating these persons from society. Self-esteem and Resilience are two important constructs enabling these persons to adapt in the society. Mostly previous studies have been conducted with respect to Self-esteem and Resilience of abled persons, however very limited studies have been done regarding locomotor disabled persons. Purpose: To examine Self-esteem in relation to Resilience, and the role of demographic variables on Self esteem and Resilience, among locomotor disabled persons. Methods: The sample size of the study is 46 locomotor disabled persons, which were selected by purposive sampling technique. The age range of locomotor disabled persons is 18 to 36 (mean =24.76). Linear regression and independent sample t-test were used to analyze the data with the help of spss-20 version software. Results: Regression model shows that Self-esteem has a strong relationship with Resilience; Self-esteem significantly predicted Resilience among locomotor disabled persons. Study also revealed a significant difference in marital status, qualification and family type, in relation to Self-esteem and Resilience. In other words, there exists a significant difference in Self-esteem and Resilience among married and unmarried locomotor disabled persons, a significant difference in Self-esteem and Resilience among locomotor disabled persons in relation to higher and secondary level of qualification, and a significant difference in Self-esteem and Resilience among locomotor disabled persons in relation to joint and nuclear family types.
452 Comparison of dusting method and fragmentation method in Laser lithotripsy approach for upper ureteric stone: Case reference study, Mohammed Ridha Joodi A.Jalo
Background: Electively, renal calculi can be surgically approached in a number of ways. Extracorporeal shockwave lithotripsy (ESWL) may be indicated to handle calculi anywhere in the urinary tract; however, it is principally indicated for upper ureter kidney stones. Ureteroscopy with laser lithotripsy may be indicated to treat calculi endoscopically and it is preferred was for ureteral calculi in the distal ureter. Objectives:: The current study was aiming at assessing the effect of laser frequency and energy power setting on outcome in the management of upper ureteric stones by semi-rigid ureteroscopy. Patients and methods: The current case reference study has been conducted in the urology department in Al-Hilla Teaching Hospital, From June the 2nd 2015 to June the 2nd 2017. The study included 46 patients with upper ureteric stones, 28 men and 18 women with an age range of 17 to 80 years. Patients were categorized into two categories. The first category, the dusting method group, included 26 patients (26 ureteric stones) who were managed using URS with laser setting (high frequency, low pulse energy and fixed irrigation system); whereas, the second group, the fragmentation method group, included 20 patients (24 ureteric stones: 4 patients having bilateral ureteric stones) who were managed using URS with laser setting (low frequency, high pulse energy and fixed irrigation system). Results: Mean operative time was lower significantly in group 2 in comparison with group 1, 40.85 ±5.34 minutes versus 50.40 ±3.21 minutes, respectively (P = 0.042). Stone freedom from single session was comparable in both groups, 21 (80.8 %) versus 17 (70.8 %), respectively (P = 0.411). The rate of double j stent was greater significantly in group 2 in comparison to group 1, 18 (75.0 %) versus 12 (46.2 %), respectively (P = 0.032). There was insignificant variation in the rate intra-operative complications between both groups (P> 0.05), in addition to lack of significant variation in the rate of early post-operative complications (P> 0.05). Conclusion: High-frequency, low pulse energy settings are more favorable for fine fragmentation (dusting) of stones especially in impacted ureteric stones, with minimal trauma to the mucosa, less retropulsion, less intra and post-operative complicationand more operative time. On other hand low-frequency, high- pulse energy settings are more effective for stone fragmentation, less operative time, more retropulsion and mucosal damage.
453 Study the role of maternal irisin levels with preterm and term delivery , Dr. Abeer Nahy Hamdy; Dr. Zuhud Mawlood Mustafa; Dr.Nihad Khalawe TektooK
In this study conducted in Baghdad for the period from February 2017 to the end of December 2017, which included 100 pregnant women who had regular contractions and / or short cervical cervices between 24 to 32 weeks of pregnancy and 100 healthy controls who did not suffer from complications Pregnancy and they were delivered on time. For the purpose of comparing the two groups with respect to the level of Irisin. The study included collecting blood samples from both groups prior to birth to investigate and measure the level of the protein Irisin in their blood serum using ELIZA technique (A Kombiotic Co, USA). Through the study, patients were also monitored in terms of the consequences of early birth in terms of complications as well as birth weight. The study showed no significant difference between preterm group (Cases) and term group regarding maternal age (P. value >0.05). The study also showed that the rate of regular contractions was 40% in patients group compared while no one of the control group was with regular contractions (P
454 Evaluating the Potential of Annona muricata L. Plant Compounds as Reducing Agents for Toxicity of Ethylene Glycol, Thaira I. Younis, Nadwah M. AL-bofkan,Wijdan Mohamed Saleh, Shihab A. Al-Bajari
Annona muricata L. is a tropical plant, which is considered as an attractive species due to its medical uses and bioactivity. This study evaluated the potential of Annonaplant for decreasing the toxic effect of ethylene glycol. Twenty five male rabbits were divided into five groups, i.e., control group (without any additives), group fed with ethylene glycol and the rest of groups were fed withAnnona muricata L. compounds (flavonoid, glycoside and alkaloids) to monitor its impact on liver activity. Results revealed that ethylene glycol increased the levels of aspartate transaminase, alanine transaminase, gamma-glutamyltransferase and alkaline phosphatase, whereas Annona compounds decreased the toxicity of ethylene glycol.A significant difference (P˂ 0.05) in total protein, albumin, globulin values as well as A/G ratio was observed in the control group in comparison to ethylene glycol group and ethylene glycol groups with Annona muricata L. plant compounds. Lipid peroxidation resulted in high level of malondialdehyde in the ethylene glycol group in comparison to the control group, however, Annona compounds reduced the level of ethylene glycol, which in turn decreased the oxidative damage. Glutathione, catalase as well as glutathione peroxidase levels increased among the groups fed with Annona compounds in comparison to that fed with ethylene glycol only. Superoxide dismutase recorded approximately the same levels in all tested groups.There was a significant difference (P˂ 0.05) in MDA and CAT values in all groups, while no significant difference (P> 0.05) was observed in other parameters, i.e., GSH, GPx as well as SOD levels.
455 Assessment of Midwives' Knowledge towards Management of Third Stage of Labour in Baghdad City, Shatha Mahmood Niazi
Background: The manage stage directly affect the postpartum hemorrhage, one of the main causes of maternal mortality in the world. The aims to assess midwives knowledge about management of third stage of labour in Baghdad City/ Iraq; and also, to determine the relationship between knowledge and midwives socio-demographic characteristics.Methodology: A descriptive study is conducted to explore knowledge of midwives regarding third stage of labour management. A convenient sample of (N=73) midwife is selected throughout the use of probability sampling approach.The data is collected through the use of a constructed questionnaire self-report midwives and analyzed through application of the descriptive statistical analysis approach that includes "frequencies & percentages"; and Inferential statistical data analysis approach used by application of the "Chi-square test".Results: The study results indicate that the (46.6%) of midwives within age (20-29) years old, (45.2%) secondary school nursing graduated, (72.6%) were Married, (47.9%) have 5-10 years of experience and (45.2%) with one session of training. The midwives educational attainment and training course have been significant relationship with their knowledge at p-value ≤ 0.05.Conclusion:Knowledge in terms of management of third stage labour, midwives were poor knowledge. More training course staff on labour stages management and distribute them appropriately by local officials help raising professionals’ midwives and high education degree which indeed helps to develop their knowledge.
456 Evaluating the Quality of Primary Health Care Services in Babylon Governorate, Iraq, Qasem Abood Kareem, Suhir Mohammed Alalawe
Background: Quality of care is considered an important aspect of the health care delivery system that must be given priority. In fact, primary health care includes providing many essential services which increase the importance of evaluating its quality.Materials and Methods: This is a descriptive, cross-sectional study conducted at 23 randomly selected (multistage sample) Primary Health Centers in Babylon governorate. Data were collected during the period starting (November 2019-February 2020).Results: The average percentage of medical units for all centers was 82.45%, and the communicable diseases control unit was 86.33%. While (78.3% and 91.3%) of PHCCs were fair regarding the presence of medical and health staff according to the standards respectively. 60.9% of the study centers had a fair score for provide all vaccines in the institution. As for dressing unit, the study found that a high percentage of PHCCs had good indicators. Conclusion: The current study shows there are clear deficiencies in standards including; health staff, administrative staff, and doctor’s examination rooms.
457 Background: Quality of care is considered an important aspect of the health care delivery system that must be given priority. In fact, primary health care includes providing many essential services which increase the importance of evaluating its quality.Materials and Methods: This is a descriptive, cross-sectional study conducted at 23 randomly selected (multistage sample) Primary Health Centers in Babylon governorate. Data were collected during the period starting (November 2019-February 2020).Results: The average percentage of medical units for all centers was 82.45%, and the communicable diseases control unit was 86.33%. While (78.3% and 91.3%) of PHCCs were fair regarding the presence of medical and health staff according to the standards respectively. 60.9% of the study centers had a fair score for provide all vaccines in the institution. As for dressing unit, the study found that a high percentage of PHCCs had good indicators. Conclusion: The current study shows there are clear deficiencies in standards including; health staff, administrative staff, and doctor’s examination rooms., Maysoon Khaleefah Abbas, Professor Dr. Suzan S. Hussain , Professor Dr.Ali H. Alwan Noor Ibrahim Khadhom
One hundred blood samples and seventy-four urine samples were collected from urinary tract patients From March to August, 2018 in five hospitals in Baghdad. The patients were divided according to gender and age, as their ages ranged from (14-65) years. E.coli bacteria were isolated and diagnosed from the urine of the patients, and all bacteria were diagnosed by biological, chemical and molecular methods, and 57 E.coli isolates isolates were obtained. The TLR-4 gene was detected in patients ’blood by PCR technique and 100% contained the TLR-4 gene. A gene expression determination was performed by qRT PCR. The results showed an increase in gene expression compared to controls. The DNA sequence was also performed for the enlarged fragments of the PCR product. They were identical to the expected amplification size in all the genes under study which yielded positive results by PCR technology and conducted a similarity search with the basic local alignment research tool (BLAST) and confirmed that these reference strains were obtained in the bank gene and was the correct identification of all samples. The relationship between the TLR-4 gene and the exogenous outer membrane genes in bacteria (Escherichia coli) was studied by detection of these genes (Smp A, BIC, proB, OmpA,) by PCR technology and these genes appeared in high proportions. In bacterial isolates excluding the OmpA gene appeared by a small percentage. ELISA was analyzed for patients' serum to detect cytokines ((INT- and TNF – α) the results showed a marked increase in cytokines compared to controls where the relationship was negative and there were significant differences between the levels of cytokines in patients and healthy subjects and there were no significant differences in the levels of cytokines between females and males. Conclusions:Sequencing for 20 PCR product of TLR-4 gene to detect matching of Iraqi population:The human gene expression of TLR-4 gene was elevated compared to controls result to trigger by Gram negative bacteria; The isolates E. coli bacteria were positive for the outer membrane genes (Smp A, BIC, proB, Omp). This has the effect of stimulating and increasing gene expression of TLR-4. Therefore, it was very effective by increasing the levels of INT-γ and TNF-α cytokines in infected patients.as to future work can be Study the course of signals when immune cells respond to pathogens, and determine the amount of cytokines secreted as a result of the TLR-4 immune responses.
458 Comparison between the Effect of Using of Oxytocin and Oxytocin with Tranexamic Acid in Reducing Uterine Bleeding During and After Caesarean Section, Fatima Saleem Maree, Masryia Rashad Hassein
Cesarean section is one of the commonest hospital based surgical procedure in obstetric mainly done to facilitate delivery in case where vaginal delivery is either not feasible or poses undue risk to mother, baby or both. The aim of this study was to compare between the effect of intravenous tranexamic acid with oxytocin verse oxytocin alone in reducing of blood loss intra and postoperative cesarean section. The study was carried out in Tikrit city (Salahadeen general hospital) from 15th of February 2019 to 15th of June 2019 . The number of pregnant women understudy were 100 women with a 18–40 years and , 50 of them were received tranexamic acid plus oxytocin infusion and 50 women who were received oxytocin infusion alone (and considered as control group). The main outcome measures were the determination of blood loss at cesarean section, change in hemoglobin levels, need for additional oxytocics, and drug-related side effects. The blood was measured by weigh and volume during two periods following placental delivery to the end of surgery and from the end of the operation to 2 hours after birth. The study showed no significant differences between the two groups regarding Age, BMI, gestational age, and duration of surgery. The study showed no significant difference between both the groups with regard to obstetrical complication and indication of CS like abnormal presentation, CPD, failure of induction, fetal distress, IUGR, previous LSCS, PROM. All the LSCS were done under spinal anesthesia. There is comparison of blood loss of case and control during intraoperative and postoperative patient. In intraoperative cases there was mean blood loss of 208.7± 27.8ml (case) whereas there was 377.9±30.5 ml (control) blood loss and it was found to be statistically significant, indicating that a major amount of blood loss is found in control group. Similarly in postoperative blood loss (in ml) it was found mean blood loss was 74.7±10.ml (case) whereas 109.4±12.6 ml was found in control in whom tranexamic acid was not administered. The study showed that the incidence of postpartum hemorrhage (PPH) i.e. ≥500 mL blood loss was lower in the study cases than in the control group.
459 The incidence of peripartum hysterectomy in Tikrit city, Rana Hadi Mosa, Sarab Salih Jasim
The present prospective study conducted in Tikrit city. Recording of all deliveries (caesarean section & vaginal deliveries) in Salahaddin general hospital and private hospitals between 1st of January to 31st of August 2019. The aim of the study was to determine the incidence, causes and outcomes of peripartum hysterectomy. All primary post partum hemorrhage (PPH) cases were recorded whether delivered inside or outside our hospital in this period. In this period, 4776 women were delivered. Analysis of patients data who had PPH regarding age, parity, setting, antenatal care, associated hypertension & anemia, PPH onset, previous scar, history of PPH or antepartum hemorrhage (APH), mode of delivery, time of arrival to hospital since delivery or referral, vital signs on admission, traditional birth attendant interference (TBA). During the study period; 4776 women were delivered; the number of cesarean sections were 1729 cases (36.2%) while vaginal deliveries were 3047 (63.8%). During this period 15 emergency hysterectomies were done and incidence of cesarean hysterectomy of 3.14/1000 deliveries. In this study, majority of women underwent cesarean hysterectomy were belonged from rural area and with age 30-39 years and parity above 6. The study found that a significant high rate (P
460 Relation between obesity complications of pregnancy and delivery, Bushra Mohammed Salih, Assistant Prof. Dr. Zuhud M. Mustafa
The worldwide epidemics of overweight and obesity have become important public health issues. The aim of the study was to screen of obese women according to their BMI and related obstetric complications and cesarean delivery. This cross-sectional study was conducted in Tikrit city over a period from 10th March to 10th July 2019 and included 100 singleton pregnant women with gestational age >37 weeks with cephalic presentation who attended Salahaddin general hospital, Gynecology and Obstetrics unit. Based on this, patients to be studied will be divided into 2 groups of 50 patients each: BMI less than 30 kg/m2 (considered as control group) and BMI ≥30 kg/m2 (obsess- cases group). All the patients included in the study were subjected to All the investigations including hemoglobin (Hb), bleeding time, clotting time, urine routine examination, prothrombin time, platelet count, renal function tests, liver function tests, blood sugar and urine for albumin were done. General examination- including general condition, hydration, blood pressure temperature, pallor, icterus, cyanosis, edema. Weight (in kgs) was measured in kilograms. Patients were weighed without shoes, wearing light indoor clothes. Height(in metres) was measured using a stadiometer. The present study showed that, out of a total of 50 pregnancies in each category, only 28% patients in the BMI≥30 category were free of complications compared with 80% of women with BMI was less than 30 (P. value: 0.004). Retinopathy, GDM and intrauterine growth restriction (IUGR) were recorded in 6% in the BMI≥30 category compared with 2% in women with BMI
461 Efficiency of silver nanoparticle against virus coronaviruses, Ali Imjayyid Hashim,Dr. Jasim Abdulaziz Al Falahy, Prof. Dr. Suzan Saadi Hussain ,Assist Prof Dr. Nihad Khalawe Tektook
Background: currently know about the Flu (Influenza) and the COVID-19 (Coronavirus) disease, which we continue to learn more about, both may present issues for the very young, the elderly, and those with underlying medical conditions. The current annual vaccines and effective antiviral drugs are not available sufficiently. Therefore, aim in current study used particles of silver as general antiseptic and disinfectant in order to prevent spread of infectious agents especially viruses. Material and methods: Maintenance of cell cultures: To determine the cytotoxic effect of Silver nanoparticles on VERO cells, the cell viability assay was done using 96-well plates. To determine the ability of silver nanoparticles in reduce the cytopathic effect of Influenza virus in VERO cells. Results: Silver nanoparticles was reduce of cytopathic effect of Influenza virus in Vero cells infected with Influenza virus (1:5 ) in the presence of silver nanoparticles at concentration 1 µM/ml. VERO cells infected with Influenza virus (1:5) in the presence of silver nanoparticles at concentration 10 µM/ml. and (1:5) in the presence of silver nanoparticles at concentration 25 µM/ml.Conclusion used silver nanoparticles to kill Influenza virus at concentration 25 µM/ml.
462 The Effect of Teaching with Mobile Learning Technology on Learning the Artistic Performance and Improving the Most Important Bio-Kinematic Variables for Rear Rolling for Standing on Hands on the Ground Mat, Manaf Majid Hasan, OrasQasim Mohammed, Jasim Nafea Hammadi
The research aims to identify the impact of the method of teaching using mobile learning technology to teach the performance of rear rolling hands to stand on the mat of ground movements for beginners using the performance evaluation index along with the biochemical variables to assist in the performance of this movement, the research used the experimental method to design a single experimental group with pre and posttests The study was conducted over three weeks and the most important results showed the achievement of Mobile Learning technology positive results to teach the performance of rear rolling to stand hands on the carpet ground movements for beginners
463 Study the effect of olive extract or refined oligonucleus on Staphylococcus aureus Isolated from different clinical cases, Noora Hamid Ibrahim, Ahmed Mohamed Turkey, Jamal Abd-al-Rahman
Thepresentstudyincludedthecollectionof100clinicalsamplesobtainedfrompatientsinRamadiwomen'sandchildren'shospital,HitGeneralHospitalandsomeprivatelaboratoriesinRamadicity.,includingBacterimeasamples,Urine,abscesses,heartinflammation,wounds,rhinitis,osteoporosis,otitis,Laryngitis,sputumdistributed(35,20,5,6,7,5,8,7,4,3)samplesrespectivelytoinvestigatethepresenceofstaphylococcusaureus. Sensitives.aureushasbeendistinguishedfromresistancebyantibioticsMethecilin,OxacilinandCefoxitine.Allisolatesshowed100%resistancetoMethecilinandOxacilinand62.5%toCefoxitine. Theresultsalsoshowedthatthealcoholicextractofoliveleavescontainedtannins,glycosides,soaps,flavonoids,phenols,resinsandaminoacids.Whileitdoesnotcontainalkaloidsusinggaschromatography-massspectrometryresultsshowedthattheextractcontainsmanychemicalcompoundsthatareofbiologicalimportanceintheinhibitionofS.aureusbacteria. TheoliveleafalcoholicextractofOleaEuropaeashowedastrongerantimicrobialactivitythantheaqueousextractagainstS.aureus,andtheminimuminhibitoryconcentrationoftheextractwas100mg/ml.
464 Determination of genetic diversity among antibiotic resistant Morganella morganii strains using RAPD molecular marks, Najat Mohammed Flyyih1*, Layla Saleh Abdul-Hassan,BasimaBasim M. A., Shrooq Ali Hussein
The study was conducted on a total of 250 clinical samples were taken from ear infection patients in Al-Sadder educational medical hospital and Al-Hakeem General hospitals, During the study period from February, 2017 to 12, 2017.This study aimed to determine Morganella morganii by using Random Amplified Polymorphic DNA ( RAPD) techniques in genetic diversity studies.The result of this study from 140(56%) samples were positive results for bacterial growth against 110(44%) samples wereconsidered negative in culture media .The biochemical test was showed that oxidase negative , catalase positive, methyl red, indole and urease positive .The percentage of antibiotic resistance was 90% , 100%, 100%,55%,100%and100% against pefloxacin , Colistin, aztreonam,ticarcillinclavulanic acid, ceftazidime and piperacillinrespectively .The result of phylogenetic tree by using RAPD analysis was similarity (0.87 ) was between (M. morganii-4 and M. morganii-12), followed by (0.80 ) between isolates (M. morganii-4and M. morganii-6 ). RAPD typing considered as a good to situations in which a large number of isolates ,cheaper and easy technique, its used in a wide range of applications, in many space of biology branch.
465 Determination of genetic diversity among antibiotic resistant Morganella morganii strains using RAPD molecular marks, Najat Mohammed Flyyih1*, Layla Saleh Abdul-Hassan,BasimaBasim M. A., Shrooq Ali Hussein
The study was conducted on a total of 250 clinical samples were taken from ear infection patients in Al-Sadder educational medical hospital and Al-Hakeem General hospitals, During the study period from February, 2017 to 12, 2017.This study aimed to determine Morganella morganii by using Random Amplified Polymorphic DNA ( RAPD) techniques in genetic diversity studies.The result of this study from 140(56%) samples were positive results for bacterial growth against 110(44%) samples wereconsidered negative in culture media .The biochemical test was showed that oxidase negative , catalase positive, methyl red, indole and urease positive .The percentage of antibiotic resistance was 90% , 100%, 100%,55%,100%and100% against pefloxacin , Colistin, aztreonam,ticarcillinclavulanic acid, ceftazidime and piperacillinrespectively .The result of phylogenetic tree by using RAPD analysis was similarity (0.87 ) was between (M. morganii-4 and M. morganii-12), followed by (0.80 ) between isolates (M. morganii-4and M. morganii-6 ). RAPD typing considered as a good to situations in which a large number of isolates ,cheaper and easy technique, its used in a wide range of applications, in many space of biology branch.
466 The Impact of Circuit Training Technique in core muscles and Legs Flexibility for Junior Football players, Prof. Bassem Khudair Abbass, Prof. Alaa Kamel Jabba, Assistant Professor Dr. Sadeq Jafar Mahmoud
This research aims to identify the impact of the circuit training technique in improving core muscles and legs flexibility for the junior football players. The researchers used the experimental method as it fits the research nature, the research sample was consisted of (30) players of Al-Shorta Football Club in football for (14- 16) years ages for the season (2018/2019).A training program was prepared involving exercises performed by the circuit training technique to improve the core muscles and legs flexibility for the junior football player. The pre- test was carried out to measure the core muscles and legs flexibility for the sample members, and then the post- test was carried out within the same procedures adopted in the pre- test, in two intermediate cycles with undulation load movement (3: 1) in the amount of (8) short cycles. Many conclusions were figured out, the most significant of which, is that the circuit training contributed in improving the core muscles and legs flexibility for the junior football players, as well as, in selecting the exercises has a positive impact on improving the core muscles and legs flexibility for the players. The researchers set up many recommendations, the most significant of which, is applying the circuit training technique based on the scientific bases to improve the flexibility for the football players.
467 Antimicrobial properties of crude extracts of Moringa oleifera against some pathogenic bacteria and fungi isolated from different sites on human body, Khamael Ali Kareem , Ammar Adnan Tuama, Intesar Kareem AbdulHassan
Moringa oleifera, or the horseradish tree, a is a pan-tropical plant, is one of approximately thirteen species belonging to the monogeneric Moringaceae family. Many reports have appeared in mainstream scientific journals describing its nutritional and medicinal properties. M oleifera was its use for the treatment of infectious skin and mucosal diseases. As it is common practice for researchers to scientifically validate the efficacy of traditional medicine, it is less common for researchers to scientifically validate simple, reproducible means of conferring therapeutic benefits of plant parts. This study was conducted to investigate pragmatic extraction techniques for seed and leaf extracts of M. oleifera, a plant species for which numerous studies have demonstrated its antimicrobial efficacy. M. oleifera seeds and leaves were extracted. Sensitivity disks impregnated with the various extracts were used for antibiotic susceptibility testing of four bacterial species and two fungal species. The zone of inhibition was used to compare antibacterial efficacy between extraction methods, trials, and controls. Conclusion, significance and impact of study: The study showed that Moringa oleifera leaves possess inhibitory properties thus can serve as an alternative therapy for wounds, certain fungal infections, bacterial, and a good source of nutrient supplements.
468 THE PROTECTIVE EFFECTS OF EXTRACT OF PUNICA GRANATUM L PEEL AND PIPER LONGUM FRUIT AGAINST HEPATOTOXICITY INDUCED BY THIOACETAMIDE IN MALE ALBINO RATS, JAFAR, S. N., MAWLOOD, K. A
The purpose of this study was to evaluate the protective effects of the extract of Punica granatum L peel (PPE) and Piper longum (PL) fruit, against thioacetamide (TAA)-induced liver toxicity in male rats. Experimental animals were divided into six groups, control, TAA, PPE200, PPE400, PL200, PL400. TAA administrated intraperitoneally, and treatment with herbal supplements was orally given for 14 weeks. Liver tissue was used for extraction and evaluating of the TNF-α gene expression and the homogenized liver tissue used to evaluate malondialdehyde (MDA) and antioxidant enzyme activities; superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GSH). Blood sera were used to measure liver enzymes. The results indicated that TAA (200 mg/kg BW) could significantly decrease SOD, CAT, and GSH whereas increased AST, ALT, ALP, and MDA (p
469 Investigation of2Adenovirus, Infleunza A and B virus infections in children2under 5 years old in Thi-Qar Province, Sara kareem, Basim Abdul-Hussein Jarullah
Background: Acute2respiratory1infection is a major killer of/children in developing3countries. Viruses are common causes of it among children and1Adenovirus, Influenza virus A and2B are some of them. Method: A2prospective cross sectional study was carried2out in children between 1-54years old with upper respiratory tract infections .Throat swabs were collected from 250 hospitalized children, and then the samples were transported by an ice bag from the hospital into the laboratory for storage in -20 ˚C. DNA –RNA extract PCR for diagnosis of viruses. Results: Adenovirus was detected in 42/250(16.8%) cases. Influenza A virus was detected in 38/250(15.2%), Influenza B virus was detected in 40/250(16%) cases. Co-infection between Influenza A and Adenovirus 8/250(3.2%) , Influenza B and Adenovirus 9/250(3.6%) ,Influenza A and B 10/250(4%), Influenza A and B and Adenovirus 4/250(1.6%). Unknown infections was14/250 (5.6%) cases. 38 of Influenza A virus-infected patients(60) were males and 22 were females, while 34 of Influenza B virus -infected children(63) were males and 29 were females , and 33 of Adenovirus –infected children (63) were males and 30 were females. Most the infections were detected in mainly less than one year old//children. Most the infections had occurred mainly//during winter and spring seasons. Conclusion: Adenovirus was more prevalent. Fever was the most common clinical signs followed by cough. The infections were prevalent in winter and early spring.
470 Selected Salivary Antioxidants and Lipid Peroxidation Biomarker in Relation to Oral Health among a Group of Dental Students, Assist. Prof. Dr. Baydaa Ahmed Yas ,Assist. Prof. Dr. Baydaa Hussein Awn
Background: Inadequate oxidant/antioxidant balance has been implicated in the pathogenesis of several oral diseases. Aims: to evaluate the relation of salivary antioxidants (total peroxidase and total glutathione) and lipid peroxidation biomarker (malondialdehyde/MDA) levels to clinical indices of dental caries and gingival disease severity among a group of dental students. Subjects, materials and methods: Study participants were forty two dental students of both gender aged 20-22 years at College of Dentistry, University of Baghdad. Stimulated salivary samples were collected and taken to the laboratory for measuring salivary total glutathione, total peroxidase and malondialdehyde. Caries experience was recorded according to Decayed, Missing, and Filled (D1-4MFS) index. Plaque index (PlI) was used for measuring dental plaque thickness while gingival index (GI) was used for diagnosis of gingival diseases severity. Results: Results revealed weak positive highly significant correlation between salivary total glutathione and D4 fraction. Whereas negative correlation was found between total glutathione and PlI that was close to the confidence limit (P=0.07). Subjects with moderate gingivitis recorded higher mean values of total peroxidase, total glutathione and malondialdehyde than those with mild gingivitis. Conclusions: Oxidative stress biomarkers analysis could be included as adjunctive tool into routine clinical screening and monitoring of oral diseases.
471 Molecular characteristics of bacteriocin produced by lactic acid bacteria isolated from local dairy products in Al-Najaf Province, Hussein Ali Hussein, Sahar Mohammed Jawad, Laith Fareed Hasan Al-Obaidi
Bacteriocins are ribosomally synthesized peptides or proteins possessing antibacterial activity against foodborne pathogens and spoilage bacteria, either within same species (limited range), or other species within a genera (wide range). In this examination, fifty isolates of Lactic corrosive microscopic organisms were obtained from matured milk and yogurt from various areas in province of Najaf. For DNA diagnosis of the isolates, samples were broke down by PCR to fast screening of Pediocin, Plantaricin and Enterocin qualities that present on the bacterial chromosome or on plasmids. Multiplex PCR technique was used to identify bacteriocin based on genes quality then the bacteriocin basic quality was obtained by intensified amplification procedure. The electrophoresis findings of the bacterial 10 strains were bacteriocin producing. Of all the obtained bacteriocin estimations, 1(2%) of 428 bp was identified to be Plantaricin, 8 (16%) with a section of 412 bp was Enterocin while 1(20%) that of 332 bp was Pediocin. The agar well dispersion technique was used for all the fifty bacterial isolates to evaluate the antibacterial activity against P. aeruginosa. Ten isolates (20%) inhibited P. aeruginosa by 14-23 mm, whereas 12 isolates were Enterocin producing and the most inhibitory to P. aeruginosa resulting in inhibition zone of 23 mm. Bacteriocins are a promising natural and safe biologic preservative for industrial food preservation.
472 Molecular characteristics of bacteriocin produced by lactic acid bacteria isolated from local dairy products in Al-Najaf Province, Hussein Ali Hussein, Sahar Mohammed Jawad, Laith Fareed Hasan Al-Obaidi
Bacteriocins are ribosomally synthesized peptides or proteins possessing antibacterial activity against foodborne pathogens and spoilage bacteria, either within same species (limited range), or other species within a genera (wide range). In this examination, fifty isolates of Lactic corrosive microscopic organisms were obtained from matured milk and yogurt from various areas in province of Najaf. For DNA diagnosis of the isolates, samples were broke down by PCR to fast screening of Pediocin, Plantaricin and Enterocin qualities that present on the bacterial chromosome or on plasmids. Multiplex PCR technique was used to identify bacteriocin based on genes quality then the bacteriocin basic quality was obtained by intensified amplification procedure. The electrophoresis findings of the bacterial 10 strains were bacteriocin producing. Of all the obtained bacteriocin estimations, 1(2%) of 428 bp was identified to be Plantaricin, 8 (16%) with a section of 412 bp was Enterocin while 1(20%) that of 332 bp was Pediocin. The agar well dispersion technique was used for all the fifty bacterial isolates to evaluate the antibacterial activity against P. aeruginosa. Ten isolates (20%) inhibited P. aeruginosa by 14-23 mm, whereas 12 isolates were Enterocin producing and the most inhibitory to P. aeruginosa resulting in inhibition zone of 23 mm. Bacteriocins are a promising natural and safe biologic preservative for industrial food preservation.
473 Advances in Drug and Vaccine Development for SARS-CoV-2: A Review of RCT data, Abubakar Yaro, Senait Kebede
SARS-CoV-2 is a novel coronavirus that has being causing global pandemic since December 2019. It targets the respiratory tract. As the time of writing this review, there is no specific treatment for COVID-19. A number of studies have proposed the utilization of some drugs as part of the repurpose strategy. This review was undertaken to evaluate the efficiency of drugs and vaccines being developed against SARS-CoV-2. It was based on synthesis of clinical research data whose results has been published. Three antiviral agents were identified as being effective and safe: Remdesivir, Lopinavir-ritonavir in combination with ribavirin. For the vaccine candidate, only one candidate was included in the review. This is mRNA vaccine (mRNA-1273) which was developed by Moderna, a US based company. In addition, new data although not synthesised but worth mentioning is the effect of dexamethasone, an anti-inflammatory drug. This review therefore recommended that glucosteroids such as dexamethasone should be combined with remdesivir. In addition, the effect of combining remdesivir with lopinavir-ritonavir plus ribavirin should be elucidated both in short and long term
474 Effect of Location and Parity in Milk Production, Service per Conception and Growth in Iraqi Buffaloes, Imad Kazem Ali Al-Zarkan, Adnan Jawad Ahmad, Nasr Nouri Al-Anbari
The research was conducted in two locations for breeding Buffaloes (the first is the Taji district / 20 km north of Baghdad and the second is the Hashemia district in Babil 90 km south of Baghdad) for the period from 1/2/2019 to 31/1/2020 on 50 female Iraqi buffalo. The aim of the study was to investigate the effect of location and the parity on the body dimensions of animals, daily milk production and milk composition, as well as the service per conception. The effect of location was observed to be highly significant (P
475 Effect of Some Antibiotics and Trigonella foenum L. Plant Extract on Bacteria Isolated From Pregnant Women , Ayyub J. Abdl-Rahmaan Al-Baytay, Abdul Kareem Fattah Omar, Riam Gany Ahmad
A total (126) sample included (76) samples administered from pregnant women and 50 samples from non-pregnant women whose ages range from (15-45) years ,results showed the samples that gave bacterial growth were (68) samples, at a rate of 54%, while they did not give (58) samples and by 46%, Positive isolates were diagnosed from pregnant women and are stap. epidermidis, stap. aureus, stap. Saprophytic As for non-pregnant women, only isolation Stap. epidermdis .The difference in the age groups response to bacterial vaginosis, where the highest percentage was within the age limits 25-35 years, with a rate of 47%, also results showed that E. coli isolates showed resistance to all antibiotics except for CIP ,and a clear difference between bacterial isolates in their resistance and sensitivity to the aqueous and alcoholic extract of Trigonella foenum and Origanum marjorana plants, as sensitivity differed with the different concentrations used from the extracts, as the highest values of the diameter of the accretions at the concentration were 100% for both plants.
476 Histological Evaluation of local application of Phyllunthus amarus extracted powder and its combination with fibronectin protein on Wound Healing of rats , Enas Fadhil Kadhim
Background: A wound is defined as a break or damage in the skin, resulting from physical or thermal damage or as a result of the presence of an underlying medical or physical condition. Herbal medicine can be called one of the branches of medicine in various forms.Phyllunthus amarus is a small herb well known for its medicinal properties and widely used worldwide. P. amarus is an important plant of Indian Ayurvedic system of medicine. Fibronectin is a major component of the extracellular matrix. It is secreted by various cells, primarily fibroblasts, as a soluble protein dimer and is then assembled into an insoluble matrix in a complex cell-mediated process. Materials and methods: Forty rats will be subjected for a surgical operation of skin of the cheek of rats. The animals were divided into control group contain 10 rats the wound was left for spontenous healing and experimental group which divided into the followings according to the applicable materials: Group I the wound defect treated with 1mg of phyllunthus amarus extracted powder daily by using spoon excavator. Group II the wound defect treated with 1mg of fibronectin protein daily by using spoon excavator. Group III the wound defect treated with 1mg combination of (0.5 mg phyllunthus amarus extracted powder &0.5mg fibronectin protein) daily. Every single group composed of 10 rats that study in two periods 5, 10 days (5 rats for each period), then the rats will sacrified on each period. Histological assessment regarding the count of inflammatory cells was performed on all studied samples with assessment of epithelial thickness and clinical consideration for wound contraction. Results: Histological findings of the study showed that re-epithelialization, wound contraction were accelerated after local application in combination group of phyllunthus amarus extracted powder with fibronectin protein at wound site as compared with other groups. Conclusion: local application of phyllunthus amarus extracted powder and it's combination with fibronectin protein was significantly effective in cheek skin wound healing
477 Evaluation of the microbial content and heavy metals of different types of fish supplied from Basra Governorate that are available in the local markets in the city of Baghdad, Assist. Prof. Dr. Hamdia, M. S. Al-Hamdani, Assist. Prof. Dr. Aliaa, S. A. Al-Fraji, Lecture Sudad, J. M
In order to conduct microbial evaluation and estimate the heavy minerals of fish supplied from Basra Governorate to the local markets of Baghdad, 10 fish carp samples were collected in February 2019. Then all samples were subjected to bacteriological tests, and the results showed that all samples were within the standard microbial limits according to the Iraqi specification, except the F5 and F1 samples, as they contained yeasts and molds. The samples examined also recorded that most of the samples were within the permitted concentrations of lead, chromium, cadmium and iron elements, except for elemental mercury and arsenic that were higher than the permissible limits within the Iraqi standard and represented by samples F4, F3 containing elemental mercury and samples F9, F7, F6, F4-F1, also containing arsenic, thus not suitable for human consumption.
478 Molecular and serological Study on Adenoviral conjunctivitis in Anbar governorate, Sara Ayad Ahmed, Thaker Mohamed H., Noor Naji Alhayani
Background: Conjunctivitis is the inflammation of conjunctiva; conjunctivitis can be divided into infectious and noninfectious causes. Viruses particularly adenoviruses and bacteria are the most common infectious causes of conjunctivitis. Noninfectious conjunctivitis includes allergic, toxic, and cicatricial conjunctivitis, as well as inflammation secondary to immune mediated diseases and neoplastic processes. Aims of the study: The current study aimed to study the role of adenovirus in conjunctivitis and its relation to some immunological parameters like Anti-adenoviral antibody IgA & IgM in infected patients. Detection of the reliability of real time PCR technique as a cut off molecular diagnostic tool in quantitative detection of Human Adenovirus species. Evaluation of the prevalence of adenoviral conjunctivitis and month distribution of infection in Ramadi, Anbar Governorate/Iraq. Patients and methods: Fifty (50) Patients were showing signs and symptoms of acute infectious conjunctivitis were included in this study during the period extended from January to August 2019. Each patient was examined by senior ophthalmologist and a conjunctival swab was taken from him properly for adenovirus investigation. Serum from patients was examined for anti-adenovirus antibody IgM & IgA by using of Enzyme linked immunosorbent assay, while swabs were investigated for Adenovirus genome detection and viral load determination by using RT-PCR technique. Teen 10 healthy individuals were included as a control group. Results: Patients within age groups of 40 years old and above were showing highest ratio in both genders. Urban Patients were showing higher ratio (64%) than rural individuals. Forty six percent (46%) of patients were showing positive titer for IgM specific for Adenovirus in their sera. Forty four (44%) of patients were showing positive of IgA specific for Adenovirus in their sera. Forty seven 47, (94%) of patients showed detectable mean viral load (3099200.200 ± 8130962) in their sera while all control group individuals were showing negative viral load results. Conclusion: Urban Adults from both genders were more affected. Some infected people are showing positive familial factor and all patients were showing positive viral load and positive titer of IgM and IgA. Highest ratio of infection was found in March and June. patients were showing optimal symptoms of adenovirus conjunctivitis particularly
479 Molecular and serological Study on Adenoviral conjunctivitis in Anbar governorate, Sara Ayad Ahmed, Thaker Mohamed H., Noor Naji Alhayani
Background: Conjunctivitis is the inflammation of conjunctiva; conjunctivitis can be divided into infectious and noninfectious causes. Viruses particularly adenoviruses and bacteria are the most common infectious causes of conjunctivitis. Noninfectious conjunctivitis includes allergic, toxic, and cicatricial conjunctivitis, as well as inflammation secondary to immune mediated diseases and neoplastic processes. Aims of the study: The current study aimed to study the role of adenovirus in conjunctivitis and its relation to some immunological parameters like Anti-adenoviral antibody IgA & IgM in infected patients. Detection of the reliability of real time PCR technique as a cut off molecular diagnostic tool in quantitative detection of Human Adenovirus species. Evaluation of the prevalence of adenoviral conjunctivitis and month distribution of infection in Ramadi, Anbar Governorate/Iraq. Patients and methods: Fifty (50) Patients were showing signs and symptoms of acute infectious conjunctivitis were included in this study during the period extended from January to August 2019. Each patient was examined by senior ophthalmologist and a conjunctival swab was taken from him properly for adenovirus investigation. Serum from patients was examined for anti-adenovirus antibody IgM & IgA by using of Enzyme linked immunosorbent assay, while swabs were investigated for Adenovirus genome detection and viral load determination by using RT-PCR technique. Teen 10 healthy individuals were included as a control group. Results: Patients within age groups of 40 years old and above were showing highest ratio in both genders. Urban Patients were showing higher ratio (64%) than rural individuals. Forty six percent (46%) of patients were showing positive titer for IgM specific for Adenovirus in their sera. Forty four (44%) of patients were showing positive of IgA specific for Adenovirus in their sera. Forty seven 47, (94%) of patients showed detectable mean viral load (3099200.200 ± 8130962) in their sera while all control group individuals were showing negative viral load results. Conclusion: Urban Adults from both genders were more affected. Some infected people are showing positive familial factor and all patients were showing positive viral load and positive titer of IgM and IgA. Highest ratio of infection was found in March and June. patients were showing optimal symptoms of adenovirus conjunctivitis particularly
480 COMPARISON OF CHEMOMETRIC METHODS FOR ASSESSING BLENDING END-POINT BY USING NIR AND RAMAN SPECTROSCOPY, Rubén Cueva-Mestanza, Celia Vargas-De-La-Cruz , Jesús Benites-Mena, Sixto Gonzalez-Elera, Faraat Ali
Blending is a critical stage in manufacturing. Therefore, a correct determination of final mixing point is necessary. In this work, vibration spectroscopic techniques, near infrared spectroscopy (NIR) and Raman spectroscopy are compared. This study has been carried out by means of a non-invasive monitoring of a blending process of a pharmaceutical blending. Difference between the Moving Block Average and Target spectrum (DMBA-TS) and Score Distance Analysis on the Main Component (PC-SDA), applying the latter in the NIR and Raman data. Different results were obtained with DMBA-ST and similar ones with PC-SDA, so although it is possible to follow the mixing by both NIR and Raman, it is necessary to take into account the method that is applied with each technique, since the characteristics of the spectra can affect the results obtained by the methods used to determine the final point of blending.
481 Development analytical methodology and characterization of circulating albumin microheterogeneity in patients with acute alcohol intoxication by Mass Spectrometry Micro Q-TOF and MALDI-TOF, Celia Vargas-De-La-Cruz1*, Jesús Benites-Mena2, Rubén Cueva-Mestanza, Moises García-Ortiz, Sixto Gonzalez-Elera
The Human Serum Albumin (HSA) is one of the most studied human proteins, and its different and multiple functions have attracted and continue to attract the interest of researchers. HSA presents biological properties, such as antioxidant and scavenging activities, binding and transport of many endogenous and exogenous substances, and endothelial regulation function. The aim of the present work was to investigate how an even exacerbated oxidative stress condition may affect HSA structure. In particular, a study on a population of patient affected by alcoholic liver disease (ALD) was performed. Oxidative stress, indeed, has been detected in most all clinical and experimental conditions of ALD. The optimized methodology was applied for a preliminary screening of HSA isoforms derived from the plasma of 10 alcoholic patients with diagnosis of cirrhosis and from 10 healthy volunteers. From the data obtained, a drastic change in HSA structure is immediately evident. First of all, the relative percentage of the native, unmodified serum albumin is dramatically reduced. Comparing this result with related works in a study on the characterization of HSA in patients affected by cirrhosis, it can be observed that in the case of cirrhosis the relative amount of native HSA decreased of 7-9% while in ALD patients the decrease was attested around 22%. This effect may be attributed to the oxidative stress conditions which lead to both the formation of new disulfide bridges with free cysteines residues and to the oxidation of HSA cysteine and methionine residues. This hypothesis is supported by the sharp increase oh the cysteinilated and oxidized isoforms.
482 Isolation and Identification of Fungi Caused Otomycosis in Samarra city and Inhibitory of some virulence gene, AreejLuay Mohammed,Abdul-hameed. M.Hamoody, Omar Raheem Khalaf AL-Obaidi
87 isolates belonging to 3 genera were obtained from filamentous fungi as well as yeasts. The highest isolates were Aspergillus.spp, with 73 isolates, followed by Candida spp. With 12 isolates. And Penicilliumspp. With only 2 isolates. On the molecular side, the study showed that the rate of the AflD-Nor gene responsible for producing aflatoxin toxins reached 90% with 27 isolated out of 30 isolated A. Flavus. Our results revealed the appearance of the PKS gene responsible for producing 60% of the Ochratoxin to A.niger with 23 out of a total of 38 isolates.
483 Isolation and Identification of Fungi Caused Otomycosis in Samarra city and Inhibitory of some virulence gene, AreejLuay Mohammed,Abdul-hameed. M.Hamoody, Omar Raheem Khalaf AL-Obaidi
87 isolates belonging to 3 genera were obtained from filamentous fungi as well as yeasts. The highest isolates were Aspergillus.spp, with 73 isolates, followed by Candida spp. With 12 isolates. And Penicilliumspp. With only 2 isolates. On the molecular side, the study showed that the rate of the AflD-Nor gene responsible for producing aflatoxin toxins reached 90% with 27 isolated out of 30 isolated A. Flavus. Our results revealed the appearance of the PKS gene responsible for producing 60% of the Ochratoxin to A.niger with 23 out of a total of 38 isolates.
484 Isolation and Identification of Fungi Caused Otomycosis in Samarra city and Inhibitory of some virulence gene, AreejLuay Mohammed,Abdul-hameed. M.Hamoody, Omar Raheem Khalaf AL-Obaidi
87 isolates belonging to 3 genera were obtained from filamentous fungi as well as yeasts. The highest isolates were Aspergillus.spp, with 73 isolates, followed by Candida spp. With 12 isolates. And Penicilliumspp. With only 2 isolates. On the molecular side, the study showed that the rate of the AflD-Nor gene responsible for producing aflatoxin toxins reached 90% with 27 isolated out of 30 isolated A. Flavus. Our results revealed the appearance of the PKS gene responsible for producing 60% of the Ochratoxin to A.niger with 23 out of a total of 38 isolates.
485 The effect of strength torque exercises in the rehabilitation of the main muscles injured by the shoulder joint for some games, Asst. Prof. Dr.Abdulrazzaq Jebur Al-Majidi1*
Injuries of the main working muscles of the shoulder joint is one of the most important problems suffered by the players of some games that are performed at the upper end of various levels, and that the development of exercises based on the principle of attribution of strength to these muscles can contribute to the rehabilitation and reactivity of these muscles after injury and the return of the injured to their sports life and for a short period, as the current training smiles from this training principle as well as delayed in the rehabilitation of the injured. They promisedto exercise according to the strength of these muscles on a sample of (spearmen, handball players, tennis players) with a large semantic muscle and pectoral, and applied these exercises at a rate of four days a week in 7-8 weeks. The researcher reached the most important conclusions, the effectiveness of the exercises used to reduce pain and increase the strength and motor range of the shoulder joint muscles.
486 Knowledge and awareness about breast cancer among male and female students in Al-Bayan University in Iraq, Khulood S. Salim, Wafaa M.A. Al Attar, Ruaa A. Jassim, Nouran T.Abdullaa
Breast cancer (BC) is the most common cancer and the primary cause of mortality due to cancer in female around the world and it comprises 18% of all women cancer. In Iraq, breast cancer accounts for approximately one third of the registered female cancer according to the Iraqi Cancer Registry and almost one quarter of female deaths from the disease. Previous studies in Iraq reported low awareness of BC among educated populations in Iraq. Objective: To assess the level of knowledge toward BC among male and female students affiliated toAl-Bayan privateUniversity in Baghdad. Methodology: 200 students have participated in this cross-sectional survey. (% 71, n=142) was female and (29 %,n= 58) was male. The mean age of participants was (21.7± 3.22) yearsQuestionnaire gathered data on respondents’ demographics, general knowledge (part A), risk factors(partB), prevention and treatment of BC(partC).In addition to their main source of information about BC. Frequency distributions and percentages were used to describe the knowledge tested within the questionnaire. Results: General knowledge of female regarding part A of questionnaire was 67% and of male was 33%.Percentage of female correct answers belongs questions in part B was 74% and 26% for male.76% of female answers in part C was correct in comparison to 23.6% of male answers.The overall resultsof knowledge among our participants was weak (71%), while the acceptable knowledge represents only 29%. The leading source of our participants’ information about BC was TV. Conclusion: This study documented unexpected low level of knowledge about BC among male and female students, so educational campaigns toward BC is strongly needed through lectures, seminars and workshop in Universities. In addition to health education programs and advertisements about this disease through media such as TVtargeted for both men and women.
487 Evaluation of Cytomegalovirus (CMV) IgM level and IgG Avidity in Detection of Primary Infection in Pregnant Women, Mohammed K. Murshid, Wisam S.Abood
Cytomegalovirus (CMV) is a leading healthcare problem associated with stillbirth and congenital abnormalities, determining the current infection along with the possible risk factors associated with cytomegalovirus infections may play a cornerstone role in preventing its complications. The aim of this study was to evaluate the accuracy of both immunoglobulin M (IgM) and immunoglobulin G avidity ( IgGA) in detection of CMV primary infection in pregnant women, to take scientific measures and procedures to reduce intrauterine transmission A total of 182 serum samples were collected from pregnant women with different gestational ages, their ages ranged from 16 – 44 years old attended to Al-Suwaira General Hospital, whichlocated 60 km southwest of capital Baghdad. All samples were tested for CMV IgM antibody using Enzyme-Linked Immuno-Sorbent Assay (ELISA), CMV IgG antibody usinga semi quantitative automated enzyme-linked fluorescent immunoassay (ELFA) by vidas instrument and cytomegalovirus specific immunoglobulin G avidity by vidas instrument. The resulte of CMV IgG was 180 (98.9)% , while the positive IgM were 5 (2.74)%. The 5 IgM positive samples and 45 IgG positive were examinedwith CMV IgG Avidity. Among 180 sera form pregnant women, 5 showed the presence of Immunoglobulin M antibodies out of which two had equivocal avidity index range. The results showed that an avidity index with the presence of Immunoglobulin M antibodies is highly suggestive of a recent primary infection.
488 Evaluation of Cytomegalovirus (CMV) IgM level and IgG Avidity in Detection of Primary Infection in Pregnant Women, Mohammed K. Murshid, Wisam S.Abood
Cytomegalovirus (CMV) is a leading healthcare problem associated with stillbirth and congenital abnormalities, determining the current infection along with the possible risk factors associated with cytomegalovirus infections may play a cornerstone role in preventing its complications. The aim of this study was to evaluate the accuracy of both immunoglobulin M (IgM) and immunoglobulin G avidity ( IgGA) in detection of CMV primary infection in pregnant women, to take scientific measures and procedures to reduce intrauterine transmission A total of 182 serum samples were collected from pregnant women with different gestational ages, their ages ranged from 16 – 44 years old attended to Al-Suwaira General Hospital, whichlocated 60 km southwest of capital Baghdad. All samples were tested for CMV IgM antibody using Enzyme-Linked Immuno-Sorbent Assay (ELISA), CMV IgG antibody usinga semi quantitative automated enzyme-linked fluorescent immunoassay (ELFA) by vidas instrument and cytomegalovirus specific immunoglobulin G avidity by vidas instrument. The resulte of CMV IgG was 180 (98.9)% , while the positive IgM were 5 (2.74)%. The 5 IgM positive samples and 45 IgG positive were examinedwith CMV IgG Avidity. Among 180 sera form pregnant women, 5 showed the presence of Immunoglobulin M antibodies out of which two had equivocal avidity index range. The results showed that an avidity index with the presence of Immunoglobulin M antibodies is highly suggestive of a recent primary infection.
489 EVALUATION OF ANTERIOR MANDIBULOTOMY AS AN APPROACH FOR SURGICAL RESECTION OF DEEP SEATED LESION, Nawres Bahaa Mohammed, Samer Majeed Mohammed, Zina Ali Daily
Anterior mandibulotomy (mandibular swing) is an essentialsurgicalapproachto tumors of the oral cavity, oropharynx and parapharyngeal space. Objectives: to evaluate the feasibility of the technique, to determine frequency of complications and outcomes. This study consisted of 11 patients surgically treated for primary tumors in oral cavity, oropharynx and parapharyngeal space which included 7 females (64%) and 4 males(36%) with a mean age of 41.3 years, range from 40–65 years; lesion size range was 4-8 cm in greatest dimension. All patients underwent planned mandibular swing approach as part of the ablative surgery for different lesion in oral cavity, oropharynx and parapharyngeal space. Preoperative work-up included mandible evaluation with panoramicradiography that provided information on the dental anatomy and the mental foramen position. The mandibular swing approach that performed for patients has been evaluated regarding the surgical exposure which was obtained and achievement of these procedures. Paramedianmandibulotomy was used in 9 cases (82%) which used for exposure of lesions in oropharynx and parapharyngeal space, while median mandibulotomy was used in 2 cases (18%) which used for lesions in oral cavity. monocortical plate and screws were used in ten cases (91%), while transosseous wiring used in one case (9%) which result in subsequent nonunion mandibular osteotomy site. In ten cases (91%) the exposure was good, while in one case (9%) the exposure was limited. these procedures allow total removal of lesion in ten cases(91%) and it alloweddebulking of one case (9%). Only three patients (27%) have been received postoperative radiotherapy as adjuvant treatment for malignant lesion. The complication related to mandibular swing approach has been assessed and it was found that complication rate through the following up period that range from 6 – 18 months was 36%. One patient had lost one of the incisors adjacent to the mandibulotomy site during the operation. One patient suffered from bony nonunion, one patient suffered from facial neuroparesis due to marginal mandibular nerve injury. The complication rate related to osteotomy was 18%. The lower lip splitting with paramedianmandibulotomy with miniplate fixation, is a safe, simple and reliable technique for providing excellent access to tumor of the oral cavity, oropharynx and parapharyngial space.
490 Role of Oxidative Stress and Cytokines in Renal Ischemia Reperfusion Injury, Maysaa A. Abdul Khaleq, Najah R. Hadi, Dalya B. Hanna, Ghaith A. Jasim
Ischemia reperfusion injury is the principal and the most severe cause of acute kidney injury. This is the result of a generalized or localized degeneration in the transportation of oxygen and nutrients to cells and the removal of waste products from kidney cells. Ischemia reperfusion injury is the main chance element for the development of persistent kidney disorder; this is explained in terms of renal or instantaneous system irregularity for more than 3 months. Throughout various clinical cases, kidneys are exposed to the ischemia reperfusion injury, interpreted by interruption of the kidney blood out glide by continued by successive perfusion development, such as resuscitation circulatory arrest, cardiac and vascular surgery, kidney transplantation and trauma. This article offers the literature's basic information to evaluate the most relevant details about the Oxidative stress and cytokines that act in renal ischemia reperfusion injury.
491 The role of vitamin C as antioxidant in prevention of preeclampsia, Nadia Saddam Fahad Al-Assady
The basis of the International Trial of Antioxidants for the Prevention of PE (INTAPP) study is based on the recognized role of oxidative stress in maternal PE syndrome10, 11. Some studies have shown that women with PE had lowered serum dosages vitamins E12, 13 and C14, 15, two powerful antioxidants. Antioxidants are molecules which, by interacting with reactive oxygen species (ROS), protect against the harmful effects of an excess of these molecules16. Two (2) studiesconducted by Chappell et al. tend to show a beneficial effect of vitamin C supplementation in women at high risk of Preport a decrease in markers of oxidative stress17, 18. The INTAPP study, for its part, is a multi-center study which aims to verify the effect of vitamin supplementation on the incidence of PE. In fact, the general hypothesis of this study is to verify whether supplementation with vitamins C in pregnant women can prevent the onset of PE. For this thesis, we hypothesized that A) the plasma concentrations of vitamins coenzyme Q10 (CoQ10) could vary during pregnancy, as much in women supplemented with vitamins C as in women not supplemented, and B) that the plasma levels of these antioxidants may be affected in women who develop PE compared to women who remain normotensive.
492 Information Expertise and its Role in Management Ability to Produce the Physical Education Lesson in the Directorate of Education in Basra, Rawaa Abdulameer Abbas, Assistant Professor Siham Hammood Sabit, Saba Qays Ghadhban
Those interested in the educational process and lesson management sought to invest the information experience with industrial intelligence and technology because of its importance in increasing the effectiveness of lesson management and developing the creative capabilities of the teacher in directing and managing his lesson. Field research for sports educational institutions that are interested in managing the lesson and preparing mathematical teaching cadres is an important issue and necessary means for identifying the most important phenomena that accompany the process of managing the educational process, as the teacher’s success in providing the appropriate conditions to provide rich and influencing experiences in managing and directing the lesson is one of the most important factors and foundations The success of the educational process. Therefore, the study aimed to get acquainted with the information experience and its role in the administrative ability to produce a physical education and sports lesson in the Basra Education Directorate. The descriptive method, using the relational method, was used to a randomly chosen sample of teachers and physical education and sports teachers in secondary schools of the Basra Directorate of Education, numbering (300) teaching and teaching. The researchers used the scale of information experience after preparing it for the research sample and conducting the scientific foundations for it and the measure of the administrative ability to extract the lesson to obtain study data from the sample and statistically processing it. The researchers reached the following conclusion: 1- Physical Education and Sports Science teachers are distinguished by their information experience and administrative ability to manage the lesson. 2- That teachers have a degree of informational experience that has a direct moral correlation that distinguishes them from the administrative ability to manage and direct the lesson effectively and influentially the lesson. Therefore, the researchers recommend the following: - 1- Using the two scales to evaluate the information experience and the administrative ability in managing and directing the lesson, within the requirements of adequate annual job performance for teachers of physical education and sports science. 2-The necessity of conducting training courses to gain information experience with modern technology and industrial intelligence, how to access and use it and the skills that it requires. 3- Attention to the administrative ability in managing and directing physical education and sports science lessons for high school teachers.
493 Role of Doppler ultrasound study in the diagnosis of breast carcinoma in detected breast Lesion (palpable & impalpable breast mass): A prospective study, Hadeel Mohamed Farook Ahmed Al Hialys
Aim: This study aimed to identify the Doppler ultrasound in diagnosis of breast carcinoma in detected breast lesion whether they are palpable and impalpable masses. Method: A total of 875 women who suffered from breast lesions (palpable & impalpable masses) were subjected to examination by Doppler ultrasonography, The current study depended on certain standards which are: lesion size, existence of blood vessels, vascularity degree regarding the other breast normal tissues, resistive index (RI), velocity of blood flow, and pulsatility index (PI) to detect malignant tissues and differentiate between benign and malignant lesion. Results: Malignant breast lesions were more vascular than the benign lesions. The RI and PI values were significantly higher in cancers , & had been shown to be a statistically significant difference (p < 0.001) was observed between the mean values of resistance index for benign and malignant lesions (0.670–0.719) respectively, there was a statistically significant difference in lesions' size among the two groups (benign and malignant) the probability value was (p=0.013). Conclusion: Histological diagnosis showed that 72% of the benign lesions, were fibro adenoma and 84% of the malignant lesions were invasive ductal carcinoma Blood vessels were identified in 94% of the malignant lesions, with increase in vascularity degree. In benign cases or fibro adenomas blood vessels can detected in 15 - 34% of vascularity.
494 Peer group support intervention in dealing with internalizing and externalizing behavioral problems of orphan children in Kashmir, Nisar Ahmad Rather, Dr. Manish Kumar Verma
The present study was experimental in nature which aimed to study the role of peer support group intervention on behavioral problems of orphan children’s. The purposive sampling technique was used to collect the sample of 32 orphan children from four orphanages of two districts of Kashmir. The pretest and post-test data were collected from the given sample. After the collection of pre-test data, peer support group intervention was given two experimental groups and two groups were taken as control groups. After the peer support group intervention post-test data were collected and analyzed for difference. Three instruments viz: Anxiety, Depression and stress scale developed by Bhatnagar, Aggression scale developed Bhardwaj and strengths and difficult developed by Robert Godwin scale were used to collect the data. Finding revealed significant difference were found of peer support group intervention on behavioral problems among the orphan children. The results recommended that Steps should be taken by the authorities of orphanage to arrange the counseling session by the counseling psychologists and enable them to live healthy life.
495 Impact of Information and Communication Technology (ICT) on Academic Achievement of Senior Secondary School Students in Mathematics, Mr. Harish Mittut
The present paper is an attempt to study the impact of ICT on academic achievement of senior secondary school students in mathematics. Eighty senior secondary students of 11th grade were selected through purposive sampling technique from an Inter College, Rudian, Badaun district, Uttar Pradesh. Pre-treatment achievement marks were considered to form equated two groups and were randomly labeled as experimental and control group. Results reveal that 1) ICT has significant and positive impact on academic achievement of senior secondary school students in mathematics as comparison to conventional method of teaching mathematics; 2) ICT has significant and positive impact on academic achievement of senior secondary school boys and girls in mathematics as comparison to conventional method of teaching mathematics. Teaching of mathematics with the integration of ICT was found more effective when compared with teaching of mathematics through general method. It is recommended that ICT integration should be there in mathematics teaching in senior secondary schools for the enhancement of students’ achievement in mathematics.
496 Job Satisfaction among Non-teaching Staffs of Secondary Schools, Ansarullah Tantry, Zaiba Ali
Objective: The purpose of this comparative study was to observe the levels of job satisfaction among non-teaching staff of Secondary Schools of Kashmir with reference to gender and marital status. Methods: A sample of 300 subjects (married=111, unmarried=109 & widow/widower=80) were selected by stratified sampling method. Dubey’s Job Satisfaction Scale (JSS) was used to explore job satisfaction of the sample. After collecting responses on job satisfaction, t-test and ANOVA were applied to explore the difference between the sample groups. Results: Findings of this study explained that there is a significant difference in job satisfaction between male and female subjects. Moreover, the sample showed a significant difference with marital status. Conclusion: It can be concluded that the levels of job satisfaction differs among non-teaching staff of Secondary Schools with reference to their gender and marital status. Implications: Job satisfaction can be enhanced among school staff by maintaining supportive work environment and creating the possible social support and respect.
497 Influence of Aggressive Media and Its Effect on Student Behaviour, Dr. Azizi Yahaya, Dr. Balan Rathakrishnan, Dr. Hanum Ahmad, Dr. Ismail Maakip1, Dr. Peter Voo Su Kiong , Dr. Mohammad Amin Wani
This study aimed to find the influence of aggressive media and its effect on student’s behaviour. The study consists 127 students (92 male & 35 female) with problematic discipline from three secondary schools in Kulai District, Johor. The instrument used in the study was a set of questionnaires divided into two parts. Part A is used to look at the background of respondents, whereas section B is used to answer all four research questions. Questionnaire items were measured using a five-step scale. The data obtained were processed using the SPSS 17.0 version to evaluate the percentage of frequency, mean, t-test and correlation test. A pilot study was conducted by the researcher to measure the reliability of the questions in the questionnaire. The findings reported moderate impact of aggressive media on student’s behaviours. Findings for the effects of students' aggressive behaviour are also moderate. This study has important implications on the role of the media in re-evaluating broadcast programs in order to minimize aggressive elements that may cause students to act aggressively.
498 An in silico approach to design potential siRNA molecules of SARS-CoV-2 Virus structural genes, a preliminary opinion for COVID-19 inhibition , Mona N. Al-Terehi, Ayad F. Alkaim,Abubakar Yaro
The major public health issue now is severe acute respiratory syndrome coronavirus 2 called SARS-CoV-2 or COVID-19 disease that detected in china at the end of 2019 and caused a large global outbreak, World Health Organization (WHO) have shown that this disease become pandemic, the information's about COVID-19 under update till now and different therapeutic strategies still suggesting , the present study introduced An in silico approach to design potential siRNA molecules of SARS-CoV-2 targeting Virus structural genes, as a preliminary opinion for COVID-19 inhibition. the structural genes included spike protein (S), envelope protein (E) , membrane protein (M) and nucleocapsid protein (N).the sequence data of SARS-CoV-2 (MN908947.3) used for siRNA designing by siDIRECT bioinformatics tools , the results show there were 177 , 539, 27 and 106 siRNA molecules of N, S, E and M genes respectively, then the choosing of some siRNA molecules Tm, low off-targets effect and mismatches nucleotides resulted 18, 24, 2 and 9 siRNA molecules of N, S, E and M genes respectively, the siRNA for the structural proteins were low Tm, low off-targets effect, targeted more than one region at same gene, and molecule sequence started from 30-100 n downstream gene beginning . Study concluded that the dealing with short genes of SARS-CoV-2 was more benefits for siRNA design and targeted more than one gene at the same time may introduce promising results in vivo experiments.
499 Expressions of Carcino-Embryonic Antigen (CEA) and Epithelial Membrane Antigen (EMA) in Oral Squamous Cell Carcinoma, Muamer Al Ashalah
Squamous cell carcinoma (SCC) is considered as the highest malignant neoplasm of oral cavity and includes few etiologicparameters applied in its development. Thus,the overall survival ratio of oral squamous cell carcinoma (OSCC) is low, mainlydue to the propensity of few tumors to disseminate through lymphatic.In this case, the results oflymph node’s involvementhave been considered as a significant indicatorof poor prognosis. During last 20 years, there are accurate investigations of valid biomarkers predictive of cancer behavior and cervical lymph node metastasis in neck and head squamous cell carcinoma. In this research, total 25 OSCC specimens from the patients with OSCC who were treated surgically in Maxillofacial Unit from Oct 2006tillApril 2009 have been included. All tests were carried out on 5μm section of formalin-fixed as paraffin-embedded OSCC specimens, then these parameters were compared to one another, as well as to the clinic-pathological features.
500 A Biological Study on Quercus Bark as Antimicrobial Agent, Israa A.A. Al Hawani, Areej H. S. Aldhaher, Iman Mohammed Abdalzahra
Quercus sp. [Oak] has been used in traditional medicine widespread around the word. in Iraq, It has been used widely as antiseptic after birth by women. The aim of this study was to evaluate the biological activity of the aqueous and alcoholic crude extracts of the bark of Quercus sp. against two types of bacterial reference strains Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 and assay the cytotoxicity of Oak crude extracts. The primary screening was carried by using well diffusion method. The both extracts [aqueous and alcoholic] showed a broad-spectrum effect on Gram positive and Gram negative reference strains. While alcoholic extracts showed the greatest effect, Gram negative were more sensitive than Gram positive. However, the minimum inhibition concentration was 100 mg/ml for both extracts against both bacterial strains. The two extracts showed bactericidal effect, the growth of the reference strains was monitored for 7 days and no growth was observed, except in the 100 mg/ml concentration of the aqueous extract, 2-3 colonies was growing back. The cytotoxic effect of the extracts was assayed on the red blood cells [RBCs], the result showed no cytotoxicity in the absence of alcohol. Thus, the bark of Quercus sp. may be used as a source of natural bactericidal agent safely.
501 Assessment of levels of Stress, Anxiety, and Depression among Raparin University Students in Rania Kurdistan region/Iraq, Blend B. Ameen
College students are more exposed to psychological problems due to changes in social life, being away from home, and new responsibilities. It can affect students’ health and academic performance if they don’t learn how to cope with it. The purpose of this study is to assess and compare the level of stress anxiety and depression among first and fourth stage students at Reparin University. Quantitative design systematic random sampling techniques were used that consisted of 295 students from the first stage 141 students and from fourth stage 154 students at different departments. The data were collected through the DSS21 questionnaire. The study shows that more than 60% of the samples were female. The mathematics department had the highest percentage of the students and it represents 17.3%. And more than 85% of students did not work, where they rely totally on their families. The study also shows the students have moderate to extremely severe levels of stress, anxiety, and depression. And there is a significant difference between the first and fourth stage students in which the levels of stress, anxiety, and depression among the first stage are higher than the fourth stage. It is recommended to supply the new student with information with an explanation of the role of universities and how they prepared to start the new life. Following up the student periodically and frequently, additionally, trying to solve their problems and decrease the load on them.
502 Antibacterial activity of Oak and Oak gall extracts, Shawqi Muhammad Hassan, Abdulqadr Khdir Hamad, Qadir Rasul Dawid, Ahmed Farhan Shallal
Objective:The objective of this study was to evaluate the antibacterial activity of methanol extracts of oak, oak gall and whole fruitagainst number of Gram positive bacteria and Gram negative bacteria. Methods: The fresh fruits extracts (Oak, oak gall and whole fruit) were separately prepared with methanolby standard procedure. The samples were stored in refrigerator at 4°C until use. The bacterial samples were taken from Microbiology laboratory at the University of Raparin, Ranya-Kurdistan Region-Iraq. The antibacterial activity of different extracts of oak, oak gall and whole fruit was determined by using disc diffusion method. Results: The results of current study showed that all methanol extracts have effect on the growth of tested bacteria, except Gram negative bacteria. The study demonstrated that there was more significant effect on growth of gram positive bacteria (Staphylococcus sp, Streptococcus sp، Bacillus sp and Micrococcus sp)based on the inhibition zone diameter. Conclusion: This study indicates that allextracts mainly whole fruit have high antibacterial activity, and may serve as natural alternatives antibiotic against variety of bacterial infections caused by multi drug resistance Gram positive bacteria . More studies are require to isolate the active antibacterial agents in oak and oak gall so that they could use in pharmaceutical purposes.
503 Association of Serum Level of tissue Transglutaminase Antibodies and Age of Iraqi Patients with Celiac Disease, Alaa S. Alattabi, Abeer Thaher Naji Al-HasnawI, Jalal Ali Ashour
Background: celiac disease (CD) is an immune mediated disorder in genetic susceptible following ingestion of gluten containing product associated with increase serum level of many antibodies such as tissue transglutaminase. Materials and methods: Retrospective cross sectional study for five hundred patients with celiac disease their serum testes for ELISA tissue transglutaminase (tTg) IgA and IgG. Results: Higher serum level of tTg IgA was more than 200IU (37%) patients and 400 (80%) patients were less than 20IU while higher level of tTg IgG more than 5 IU in 222 (44%) patient. Conclusion: There was a significant relationship between tTg IgA serum level and age in celiac patients. Also, this study was reported a non-significant correlation between tTgIgG serum level and age of these patients.
504 Evaluation of Laparoscope in Abdominal Trauma in Kerbela city, Abdulrazzaq Kalaf Hassan
Background: The use of laparoscope in trauma for diagnostic purposes is generally limited; we attempt to investigate the therapeutic function of laparoscopic surgery in trauma patients. Laparoscope may be effective and safe in treating patients with abdominal trauma. Aims of the Study: To identify the following: 1. Benefits of Laparoscope in Abdominal Trauma. 2. Contraindications for Laparoscope in Trauma Patients. 3. Indications for conversion (from laparoscopic to open approach). Patient and methods: forty patients with abdominal trauma whether penetrating or blunt who were admitted to the casualty unit in Imam al-Hussein Medical City in Holy Karbala City from Jan 2016 to June 2016 and were diagnosed as a cases of acute abdomen by clinical examination base some were in shock state and unstable admitted immediately to theatre laparotomy was done to them other cases in shock but corrected undergone surgery for different causes some of them undergone purely laparoscopic interference by laparoscopic device under the name (KARL STORZ—ENDOSKOPE) other patients undergone conversion for different causes. Results: forty were included in a prospective study who were undergone surgical intervention either in form of traditional laparotomy or diagnostic laparoscopy eight (20%) of them undergone exclusive laparoscopic interference while nine (22.5%) undergone conversion , other twenty three (57.5%) undergone open approach. Conclusions: From the study, it was concluded that: 1. One of the most important tools that is used to manage the blunt abdominal injury (BAI) is laparoscope. 2. The laparoscope is a good preventive measure of nontherapeutic laparotomies. 3. It’s important measure in diagnosing and even treating diaphragmatic injuries. 4. Traumatic bowel injuries can be diagnosed and even treated by the laparoscope 5. The presence of profuse hemorrhage make continue on laparoscopic approach is non judges.
505 Effect of dialysis on some Hematological and Electrolyte parameters in chronic kidney patients , Jangy Esmail Abdulla, Jamal K. Shakor, Ahmed FarhanShallal,Ramiar K. Kheder
Objective: The purpose of the current study was to estimate some of the blood components and electrolytes on renal failure patients. The hematological parameters included measurement of Total WBC count, Lymphocyte count (%), MID cell (%), Granulocyte (%), RBC count, HGB, HCT (%) and platelet count while the electrolyte aspect involved serum levels of Calcium, Sodium, Potassium, and Chloride. Methods: In this study, some hematological and serum electrolyte of 30 patients with end-stage renal disease were measured before and after hemodialysis. Blood elements were detected by using the auto-analyzer system. Fujifilm - Dri-Chem NX500i analyzer was used to detect the serum level of electrolytes. Results: The current study showed that the dialysis had a clear effect on the blood elements, some hematological parameters increased includingMID cell (%), RBC count, HGB, HCT (%) However, Total WBC count, Lymphocyte and Granulocytedecreased after dialysis. Some serum electrolytes were analyzed, calcium and sodiumlevels increased significantly (P
506 Prevalence of SARS-CoV-2 (COVID-19) in Raparin district, Ahmed Farhan Shallal, Omed Hassan Qadir, Ramiar Kamal Kheder,Jangy Esmail Abdulla,Jamal K. Shakor, Rawand Abdulrahman Isa
Background: The COVID- 19 disease linked to the severe acute respiratory syndrome coronavirus virus 2 (SARS-CoV-2), it is a pandemic virus which has high fatality rate. Social gathering is being proposed as a risk factor for the disease. The aim of this study was to find out the level of Coronavirus (COVID-19) infection in Raparin district/Sulaimania governorate/ KGR, Iraq. Materialsand Methods: The Five hundred and two nasopharyngeal swab samples were collected from different screening programsduring the second and third month of the year 2020. The samples randomly were collected from people who are in high-risk placesfor spreading COVID-19 such as petrol pump stations, markets, and pharmacy stores. Nuclear material was extracted using the PowerPrepTMViral DNA/RNA Extraction kit(E0007). Real time PCR was used using PowerChekTM2019-nCoV Real-time PCR kit. Results: The current studydemonstrated that the mean age of the screened population was 34.45±12.55,the majority of whom were male (81.7). The screened population was mostlyfrom Iraqi Kurdistan (68. 3%),while other samples were tested from the people who came back from other countries(20.2%). Overall surveyed people were asymptomatic and not presented with other morbidities. The prevalence of COVID-19 among the surveyed population was 4/1000 population. Conclusions:Through the results of the current study, our conclusion wasthat quarantine and social separation, in addition to the curfew, are the main factors in preventing and spreading infection with Coronavirus.
507 The effect of exercises using the Cogni Plus device on developing perception of handball referees, Raafat Abdulhadi Kadhim, Hikmat Al-Lami, Khalid Shakir Hussein
The study aims to use modern technology in developing handball rulers using exercises of the Cogni Plus device and to identify the effect of exercises in developing perception of the handball rulers. The study hypothesizes that the exercises of the Cogni Plus device have a positive effect on developing perception of handball referees. The researchers used the experimental method in the one group method to suit the nature of the problem studied. The study community identified the handball referees accredited in the Central Iraqi Handball Federation for the sports season 2016-2017 and they numbered (43) first-class rule workers, and handball referees were chosen in the Middle Euphrates region In Iraq, in the simple random method, where the number of the sample became (10) rulers, the arithmetic circles of the perceptual variables ranged, so the arithmetic mean for the total field of vision (169.1±1.203), visual focus (49.184±1.14), divided attention (79.381±1.242), and emotional maturity (1.824±0.081) and response time Verb (0.682±0.021), after prolongation The direct awareness of the Vienna system and its devices, the researchers chose the perimeter perception test and the Alertness - phasic s1 program was selected. Through the use of the test, the researchers were able to identify the exact shape of the device, how to conduct the test, and the reliability of the results obtained. The pre-test was carried out and the curriculum was implemented The training that took (8 weeks) and after completion, the post-test was conducted. The results of the study were presented and discussed. The most important conclusions were that the exercises of the Cogni Plus had a positive effect on the development of perceptual variables, namely (total field of view - divided attention - visual focus - visual reaction - emotional maturity) among handball referees.
508 Salivary Protein Carbonyl Level in Relation to Gingival Health Status among a Group of Iraqi Pregnant Women , Assist. Prof. Dr. Baydaa Hussein Awn B.D.S., M.Sc. and Lecturer Dr. Shahbaa Munther B.D.S; M.Sc. , Assist. Prof. Dr. Baydaa Ahmed Yas B.D.S., M.Sc., Ph.D.
Background: There is an increased oxidative stress during pregnancy that might affect their gingival health. This study was conducted to measure salivary protein carbonyl level of pregnant women and its relation with gingival health also compared the data obtained with those from healthy married non-pregnant women. Material and Methods: Thirty pregnant women during third trimester (i.e. study group) and thirty married non-pregnant women (i.e. control group) were selected from the Primary Health Care Centers in Baghdad city. The age range of both groups was 25-30 years. Stimulated saliva was collected and salivary protein carbonyl was analyzed using protein carbonyl assay kit. Plaque index (PlI) was used for measuring dental plaque thickness while gingival index (GI) was used for diagnosis of gingival disease. Results: The mean values of salivary protein carbonyl, plaque and gingival indices were highly significantly higher among pregnant than non-pregnant women (P˂0.01). Person's correlation coefficient showed positive strong highly significant correlation (P˂0.01) between plaque accumulation and gingivitis among pregnant and non-pregnant women. While non-significant correlations (P>0.05) were recorded between salivary protein carbonyl levels with both gingival and dental plaque indices among both pregnant and non-pregnant women. Conclusion: Although dental plaque is the main etiological factor in pregnancy gingivitis, protein oxidation represented by salivary protein carbonyl level could play a role in gingival inflammation during pregnancy. Therefore examining the proteomic profile along with protein oxidation is recommended that helps in early detection and monitoring of periodontal infection during pregnancy.
509 Medical Devices, Tools, and Equipment Surfaces Contamination in Three Departments at a Tertiary Hospital in Baghdad, Sarab K. Alrawi, Manar S. Assi, Zakaria Y. Younus, Ali H. Hayyawi, Abdulrazzq W. Abdullah, Ban N. Lateef, Yahya A. Fouad
The hospital environment, medical instruments and health care staff considered as a reservoir of potential pathogens that colonized by microorganisms which can cause infections. Bacteria could be survived up to months on dry inanimate surfaces and could be transmitted to the patients or visitors through the hand when contact with contaminated medical devices. The study was achieved from June 2017 to December 2019, at the most three occupied departments at Teaching Hospital in Baghdad. Swabs were collected from medical instruments and surfaces. Samples were cultured and incubated for bacterial and fungal pathogens respectively, through the standard microbiological procedures. From a total of 1043 swab, the highest samples were collected in the year of 2018(455) swab, as well as, the highest percentages of bacteria were isolated. Mixed bacterial growth was isolated from 82 swabs (7.86 %), while 961 swabs (92.13%) did not show any growth. the isolates had 54 (65.85%) Gram-positive bacteria, which were Bacillus then Staphylococcus epidermidis (28 and 11) isolates respectively, while 28 (34.14%) were Gram-negatives, that consisted of Pseudomonas aeruginosa 13 isolates, followed by Escherichia coli 11 isolates. The Gynecology Department carries higher rates of bacterial contamination (50%) and the Ultrasound units in both of (Gynecology and Surgery) Departments revealed a high percentage of contamination (24.39%, 29.16%) respectively. In conclusion, the contamination rates with Gram-positive bacteria increased even after regular cleaning and disinfection in the environment surrounding patients and medical devices in the hospital.
510 Studying the impact of vitamin D deficiency in Iraqi acromegalic patients and its relation with some biochemical parameters , Amer Hasan Abdullah, Ibtisam Kareem Mohaisn, Aufaira Shaker Nsaif, Abeer H. M. Safaryan
Acromegaly is described as a less common chronic disease occurs as a result of over secretion of growth hormone (GH) often from a pituitary adenoma; it is connected to noticeable morbidity and increased mortality. Our study aimed to determine calcium (Ca) and vitamin D status in acromegalic patients regarding disease activity and estimating the relationship between vitamin D, IGF-1 and some biochemical parameters. Correlations of vitamin D with IGF-1, BMI, Ca, GOT, GPT, ALP, T3, T4, TSH, urea and creatinine were studied. The study groups comprise of 50 male, 25 acromegalic patients and 25 control male groups. The results of vitamin D measurements showed a decrease levels of vitamin D in all acromegalic patients and this revealed that patients with acromegaly have a high significant decrease (p < 0.001) of BMI, IGF-1, vitamin D, Ca, GOT and creatinine compared to control group, while significant increase for GPT, ALP, T3, T4 and urea for patients with acromegaly. However, the results showed a highly positive significant association between vitamin D and BMI, IGF-1, urea, creatinine and T4 levels, whereas demonstrated a highly significant positive correlation of vitamin D levels with GOT and ALP, also the results showed non-significant difference between vitamin D, Ca and T3 with a negative correlation with TSH also asignificant positive correlation between vitamin D and GPT appeared. We concluded that acromegalic patients were suffering from low vitamin D levels compared to the controls group and also suffering from lower kidney activity (hydroxylation at the position of 25-OH-D3 site) and this is evident from the high creatinine levels in the blood, which leads to the non-activation and even low levels of vitamin D and therefore acromemglic patients will suffer from vitamin D deficiency high risk thus the treatment procedures for acromegaly should include doses of active vitamin D, which reduces the risk of this deficiency.
511 Anthropometric, adiposity, and lipid profile indexes as discriminators and predictors of insulin resistance in women with polycystic ovary syndrome, Suaad Muhssen Ghazi, Ismail Ibrahim Hussain
Polycystic ovary syndrome (PCOS) is an endocrine systemic disease characterized by obesity, dyslipidemia, and insulin resistance. Taking into consideration these characteristics, we decided to determine the discriminators and the predictors of insulin resistance (InsR). A total number of 50 healthy subjects (Group I) and 100 PCOS women (Group II) were included in a cross-sectional study. Anthropometric measurements, adiposity indices, and the fasting serum lipid profile, glucose, and insulin were determined. The predictors and discriminators were identified by using the multi-variables regression test and the receiving operating characteristics. Group II has significant high values of body mass index (BMI), adiposity indices, fasting serum profile and homeostasis model assessment of IR (HOMA-IR). Anthropometric measurements, adiposity indices, and serum lipid profile by 51.2%, 43.5%, and 23.2%, respectively. According to the value of the area under the curve, the discriminators of InsR in Group II were: body mass index > adult body fat percentage > lipid accumulation product > waist to height ratio > total cholesterol > Non-high density lipoprotein-cholesterol > triglyceride. In conclusion, body mass index, waist to height ratio, body fat percentage, and lipid accumulation product can serve as discriminators as well as a predictor of insulin resistance in women with an established diagnosis of PCOS.
512 Effects of heavy metals pollution on human health , Aqeel M. Ali Al-Lami, Saba Riad Khudhaier, Omer AbdulKareem Aswad
The presence of foreign materials in an environment, leading to cause undesirable changes called pollution, pollution can occur in water, soil, and air. Many other types of pollutions include thermal, radioactive, and noise pollutions. Chemicals like heavy metals usually most pollutants effects on plants, animals, and human health, cadmium exist by the volcano, fires in the forest, has many bad effects on human health, targeting the kidney, liver, and vascular system. Chrome, lead and manganese, cases of irritation, problems in pulmonary works, stomach, small intestine, and attacking of reproductive system. Cobalt and nickel cause chromosomal efficiency defects. Many big cities in the world suffer from high pollutions. Therefore these chemicals must be kept in safe limits, industrial cities must be under governmental laws, periodical examinations for humans and workers.
513 Effects of the systemic administration of omega -3 polyunsaturated fatty acid on experimental periodontitis (immunological analysis for rat’s serum), Aveen Ageel Jalal, Zewar A.Al-Qassab, Rafel A.AL-Rawi
Previous studies had been reported that IL-1, IL-6, and TNF- α were associated with various bacterial infections, including periodontitis. The present study was aimed to investigate and evaluate the effects of eicosapentaenoic acid (EPA) on ligature induced periodontitis in rats through the immunological analysis for rat’s serum level of IL-1β, IL-6, and TNF-α. The periodontitis was induced for the studied animals by ligation around the upper central incisor. Twenty-five animals were used as a control and gavaged by water only, and one hundred animals with the induced periodontitis were divided into four equal groups according to the treatment used: Water, scaling/root planing (SRP), 60mg/kg EPA, and SRP together with EPA. Blood was taken by cardiac puncture, three hours after ligation removal (day zero), 24 hours, three days, one week and two weeks for immunological analysis. The result showed that the treatment of the induced periodontitis by SRP together with 60mg/kg EPA cause a significant decrease in serum IL-1β after 24 hours from the treatment, but a significant decrease in IL-6 and TNF-α were seen after three hours in comparison with the periodontitis group treated by water only. As a conclusion from this study, the treatment of periodontitis by SRP together with EPA is better than SRP or EPA each one alone.
514 Bactericidal effect of silver nanoparticles and He-Ne laser on bacterium Escherichia coli in vitro , Khalid I. Riah
This study was designed to show the effect of silver nanoparticles (Ag-NPs) or He-Ne laser each of them individually, on Escherichia coli, also study the combined effect of nanoparticles and laser energy to kill or reduction of bacterial growth. Both, He-Ne laser and Ag-NPs were used against E. coli. Various concentrations of Ag-NPs have been employed, as far as; different irradiation times of laser were used for reducing bacterial growth. The results showed that E. coli was affected by laser and Ag-NPs when used separately and that effect was increased when using them together.
515 The Effect of Educational Exercises on Developing Upgrade Step (hopscotch) of Jumping to Perform the Open and annex Jump Skills on the Jumping Horse Device, Dr. Assist. Prof. Murtadah Mohsen Abed, Dr. Lect. Saif Alaa Naji
The researchers noticed the presence of confusion in the final steps (hopscotch) of the approximate run before performing the jumps on the jumping horse device, which make the researchers to prepare exercises to learn the hopscotch steps to perform the jumping skills of open and annex on the jumping horse in the artistic gymnastics for men. The researchers used the experimental method for its relevance and research nature. the sample of the research was deliberately selected and they are third stage students in the branch of theoretical sciences in the Faculty of Physical Education and Sports Sciences at the Maysan University with the number (45) students (23) students for the experimental group and (22) students for the control group and the researcher use skill test as a research tool.
516 The Addictive Personality and Its Relationship to Aggressive Behavior Of Middle School Students, Assist. Prof. Dr.Sufyan Saeb Al-Madhidi
Drug addiction is one of the problems facing global societies in general, and addiction in its forms has become a global dilemma that governments are trying hard to work to treat and combat. The addictive personality is the personality that deals with prohibited substances in a way that affects public behavior and character building.This comes in conjunction with aggressive behavior, which leads to self-harm and others that coincide with drug use. Although the subject in Iraq is not at a level that scares researchers in this field, it must mark the green light to stand when the situation is and try to stop it when it alone and treat it.The researcher has prepared two measures to measure addictive personality and aggressive behavior. After performing the necessary statistics for this, the two measures were applied to a sample of junior high students in Baghdad governorate. The researcher has reached the results of his research, and a number of recommendations and proposals.
517 Psychological Exhaustion and Its Relationship To Homework For Primary School Pupils, Assist. Prof. Dr.Sufyan Saeb Al_Madhidi
The homework is one of the most important basics of the educational process in the primary school, as the student have homework from school to home, which makes him feel tired and exhausted.Psychological theories and various psychology schools emphasized the importance of the psychological needs in education and teaching, as well as psychological and educational studies that emphasized the impact of exhaustion on mental and physical abilities and its effect on the internal and external psychological balance.The researcher prepared a scale of psychological exhaustion according to the statistical methods used in psychological and educational studies, and applied it to a sample of sixth-grade primary pupils in Baghdad Governorate. It consisted of 1090 male and female pupils.The research results confirmed the high level of psychological exhaustion in the lives of pupils, and the clear impact of homework on what pupils suffer from psychological exhaustion, the research results also found that there was no statistically significant difference between males and females in the level of exhaustion pupils, and the results of the study agreed with the results of previous international studies in this regard.The researcher reached to number of recommendations and proposals.
518 The Impact of the Use of Educational Programs in Acquiring the Art of Performance and Accuracy of Achievement of Some of the Basic Skills in Ground Tennis Students of the Faculty of Physical Education and Sports Sciences, Majid Khaleel Khamees,Ammar Jabbar Abbas,Bashair Raheem Shallal
Through the follow-up to the researcher for the games held by the Central Federation and sports clubs and university tournaments, and briefed him on the progress of the exercises and lectures of many university teachers in the faculties of physical education and sports sciences in the universities of Iraq, and through his field experience - as a practitioner of the game and has many achievements of studies and research - found that There are deficiencies in the preparation of educational programs for college students in universities, as well as the weakness of the level of skill which constitutes a high proportion of the needs of students in the teaching, and differences in the views of some of the teachers on how to start the process of education and training of students, That the method of applying the exercises in the formal arena is the best, and others see the method of applying exercises on the wall is the best, and some of them use the two together and no one knows what is the best method of application in teaching students to the basic skills of ground tennis, and the importance of research as an attempt The researcher used the experimental method. The research community consisted of (50) students. The sample of the research consisted of (30) students repretransmittering three experimental groups, each consisting of (30) students. Group (10) students.
519 The Effect of a Training Program on Sandy and Solid Surfaces to Know the Development of Defensive Skills in Volleyball,
Lect. Dr. Serdar Hkem Mohamed Ameen,Dr.Abduljabbar Kareem Allaw , Lect. Dr. Ali Hamid Ali
520 Cynicism And its Relationship with Moral Exclusion Among University Students, Assist. Prof. Dr. Natik Fahal Al-Kubaisy
: The environment is one of the important factors in formation and figuration the human behavior, and the the Iraqi individual’s experiences are rich of negative factors that were formed by many factors: growth of terrorism and financial and administrative corruption, an increase in juvenile delinquency and begging, prostitution, anxiety about the future, loss of motivation, and a heightened sense of isolation caused on the absence of connectionof the outside world, cultural and scientific deterioration, with the exploitation, social violence, theft, and family collapse, Cynicism within societies and organizations has aroused the interest of researchers and academics recently The current research aims to identify : 1.The level of cynicism among university students, and 2. The difference significance in the Cynicism according to the gender variable (male-female) among university students, 3. The level of moral exclusion among university students, and 4. The difference significance of the in moral exclusion according to the gender variable (male-female) among university students.The total score obtained by the respondent (student) on the scale ofCynicism in the current research.
521 Impetigo in Iraqi Patients, Yahya Ali
The goal was to compare the data obtained from the study with other studies. A total of one hundred fifty patients were seen randomly at Al-Hussein Teaching Hospital, Department of Dermatology in Thi-Qar city during the period from Nov 2016 to Oct 2017 A full history from all patients was obtained including name, age, sex , duration of the illness, socioeconomic status and the presence of constitutional symptoms. RESULTS: A hundred and fifty patients were analyzed. The Non-bullous impetigo form found to be the commonest variants encountered in this study, (144) patients making (96%) of the total affecting chiefly patients aged (6) months to (48) years, followed by bullous impetigo(6) patients constituting (4%) affecting chiefly patients aged (6) months to (2) years .Regarding non-bullous impetigo, the study showed that (77) patients constituting (53.5%) of the cases were male and (67) patients constituting (46.5%) were female, their age ranged from (6) months to (48) year with a mean age of (6.7) year. The periorificial sites were involved in (50%) while the non-periorificial sites in (38.2%) and both sites involved in (11.8%) of cases. The peak age incidence was in the first half of the first decade of the life which contribute to (54.2%) of the total. Discussion: Impetigo is a one of the common skin problems the greatest burden of impetigo is in children, with steady decreases in prevalence with increasing age. This study showed that the peak age of non-bullous impetigo occurs during the first half of first decade of life. It is well-known that non-bullous impetigo is the commonest type of impetigo. Male children are slightly more affected than female, studies which showed that nonbullous impetigo was the most common form of impetigo and that Staph. aureus was the main bacteria that causes nonbullousimpetigo, no patient has poststreptococcal glomerulonephritis complication.
522 Nephelometric quantification of IgG in gingival blood from health to early disease (experimental assay on human), Abdullatif A. Aljuboury, Raghad H. Al-Ani Zahra'a R. Mohsen
We are dealing with the plaque-induced gingivitis as an experimental assay on young adult human volunteers. Creating gingivitis by withdrawn the habitual daily oral hygiene for 25 days, then re-establishit, no professional scaling done, for another 25 days to take three samples from circulating gingival blood as a closest point to the site of infection, the col-du-sac. Suggesting that this site is the field of antigen-antibody reaction. Analyses with immuno-nephelo- metriclaser showed a positive correlation between BOP and IgG but insignificant. Our result suggestthat the circulating gingival blood could bea sour of studying the immunologic and inflammatory alteration during the gingival inflammation in reality as best as the gingival crevicular fluid.
523 Stages of Microtrauma In Iraqi Athletes and Ways to Reduce Their Incidence and Severity, Sameerah Abdulrasool Kadum, Gomaa Mohammed Awad
The research aims to study the microtraumato which athletes are exposed and that arise from the high exercises thatthe athlete exerts that exceeds his physiological ability, which leads to microtraumain the areas of contact between the tendons and muscles especially in most areas of the body, as well as informing coaches and athletes of the seriousness of these injuries to prevent their recurrence and exacerbation which affects public health and sports level.The researchers assumed that most of these injuries occur in the knee joint and in the macrophage’smuscles of the thighs, then analyze and scan records of the Specialized Sports Medicine Center in Baghdad for the years 2015-2016/2017-2018.The sample included (965) nine hundred and sixty-five patients who recheck the aforementioned specialized center where they suffering the pain and inflammations caused by these injuries at various areas of the body, where distributed (681) from the athletes(284) who are not athletes.Some information related to the causes of injuries was extracted and collected directly from the athletes by means of an information form prepared for this purpose.The injuries were distributed to the joints of the body, especially the knee, shoulder, ankle and elbow joints, the front muscle of the shin, the macrophage’s muscles of the thighs and other areas.The degree of infection also varied from one case to another, and the first stage was the most common form of capillary hemorrhagic injury and inflammation, followed by the second stage of fibrosis and then the third stage of calcification.There are many causes for the occurrence of these injuries, including high exercises and the repetition of certain movements with no legalization of training programs according to the athlete individual's abilityand his physiological abilities to withstand the performance and the burden of training and not to give full rest, as well as the ground for the stadiums which is one of the important reasons for these injuries.The researchers recommended that the training programs should be codified according to the capabilities of the individual, pay attention to periodic checks and review the physician therapist
524 Immunoregulation of cytokine signalling network in Toxoplasma gondii infected-women, Alyaa Abed Alhussein Hafedh1, Ibrahim Faris Ali, Nuha Jabbar Abed Alrikaby
The global risk of toxoplasmosis in human is dramatically rising up. Indeed, the disease is asymptomatic in human, but its risk can increase in pregnancy and immunodeficiency disorders.Cytokine profiling seems to be fundamental in determination of immunestatus of host duringinfection by pathogens, where the immunoregulation of host cytokine responses that occurs determinates the pathogenicity ormaintenance and homeostasis of pathogen-infected organs and tissues. For investigating cytokine profile during toxoplasmosis, 50of Toxoplasma gondii-infected-women and 50of Toxoplasma-uninfected women (naivecontrol group) were enrolled in this study. Interleukin 10 (IL-10), IL-11, IL-17, and tumour necrosis factor-beta (TNF-β) concentrationsin the serum were monitored using enzyme-linked immunosorbent assay (ELISA) technique in both women infected and uninfected withToxoplasma gondii parasites.In our study, the data indicated disruption in production of cytokines in Toxoplasma-infected women in comparison with naive control group. Generally, ourfindings revealed an increase in the level of all cytokines that included in this study. Noticeably, this increasingwas only significant in IL-10 and IL-11 concentrations comparingto Toxoplasma-uninfected control group at p
525 The Effect Of Interfering Attacking Physical Exercises In Developing The Aggressive Attacking Behavior Of Football Young Players, Khalid Mohammed Ridha, Mohammed Bachay Atiyah, Karrar Mohammed Mohsi
The research goals to achieved which preparing these exercises and identifying their effect in developing the research variables, the researcher assumed that the planned exercises had a positive effect on the research variables, and the study research community included Karbala club players for the youth category for the sports season 2018 - 2019, The research sample was chosen by random method (Draw), the researcher used the experimental research approach and the two equal groups method so that the research sample was divided into two equal groups, a control group and an experimental group, the training lasted for a period of 8 weeks and by 3 training units per week. Through the work, the researcher reached several conclusions, among them, that the combined attacking exercises according to the miniature areas had a positive effect in developing the level of aggressive attacking behavior as a result of the practical applications that were applied to the players.
526 Antibacterial activity and medical properties of Witch Hazel Hamamelis virginiana , Talib F. Abbas , Mosa Fadiel Abbas, Ali Jarad Lafta
While the final proof of efficacy in common dermatitis such as atopic dermatitis was found still lacking, is the authors found fairly ample evidence for their activity against cutaneous inflammation in man, as may be deduced from experiments with normal volunteers and unwanted effects related to the drug are virtually absent.Witch hazel extractions applied to dermatological areas topically provide a calming effect through tannin extraction, therefore also utilized in cosmetics as well as therapeutically.Active components of hamamelis extractions may be traced to flavonoids, leucoanthocyanidins, tannins, and essential oils that provide benefit to blood circulation, antimicrobial activity, and antioxidant activity. This research aims to evaluate of biological activity of the herbal substances of Hamamelis virginiana L., bark, leaf and twigs and preparations thereof for the categorization as products under well-established use or traditional use and the establishment of the corresponding Community herbal monographs. Determining the best method of extraction, prepare the extracts, include milling,extraction of active compounds in soxhlets ,evaporation of the extract to form semi-solid extracts , then analyzing the functional groups in FTIR instrument, and determine the biological activity by measuring minimum inhibition concentration (MIC).The results illustrate that witch hazel extracts in three concentrations (10mg/ml,50mg/ml, 100 mg/ml) have an inhibition effect on 10 plates of Candida albicans, with optimum concentration (50mg/ml).Staphylococcus aureusshowed that the higher concentration of 100 mg/ml has an optimum effect and wider radius of MIC (0.54cm±0.5). E. coli seems to have a similar effect to S. aureus biological test. The concentration of 100mg/ml showed great inhibition effect to the bacterial growth in 24 hours. Which hazel has a deteriorating effects on both Gram positive and Gram negative and fungi, a preferable actions are due to functional groups especially the carbonyl group, which has a strong effects since it’s a form of aldehyde, with the alkyl and ketone groups all have the degradation effects on the microbial cell wall, chelating agents making them perfect disinfectant, in addition to its antioxidant effects, and blood veins constructive.
527 Genotypic Description of Certain Virulence Factors in Isolated Strains of Escherichia Coli in Women with Urinary Tract Infection, Najlaa Abdullah D. AL-Oqaili
Urinary tract infections (UTIs) are one of the most prevalent contagions world and apathogenic Escherichia coli (UPEC) is the leading bacterial cause of UTIs. The intensity of UTI reflects the infection strain's virulence profile, and the frequency of virulence factor expression is larger in more pathogenic strains.E.coli isolates were diagnosed based on cultural, biochemical tests and diagnosed with API 20 E, also using the polymerase chain reaction, In addition to molecular diagnosis to investigate the 16s rRNA gene and the virulence genes that enable it to cause urinary tract infection. Also isolates were examined for resistance to 8 antibiotics by using the disk diffusion method. The infection rate for E.coli was (46.66%,21/45) from women with UTIs. The current study showed that E.coli isolates have different antibiotic resistance include: Cefalothin 85.71%, trimethoprim-Sulfamethoxazole 80.95%, nalidixic acid 76.19%, norfloxacin 66.66% and Ciprofloxacin 52.38% but the bacteria's resistance to other antibiotics was mild included: gentamicin 28.57%, amikacin 23.80% and nitrofurantoin 14.28%.The results also showed the presence of the virulence genes were fimH and usp (92.85%), cnf-1 (28.57%) and ibeA (21.42%).This indicates that there is a common relationship between urinary tract infections, the virulence genes of E.coli and their resistance to antibiotics.
528 Molecular detection of new Bacillus strains from soil samples of free grazing areas in Basrah province, Southern Iraq, Murtakab Y Al-Hejjaj, Saad S Al-Amara, Yessar A Dawood, Shaymaa Jabbar Raisan, Hassan M Al-Tameemi
Bacterial contamination is an important indicator for soil quality that could be related to the plant’s suitability for animals ‘grazing and ultimately human public health. Consequently, the microbial characteristics of soil could be linked to animals’ products quality, such as milk and meat. Bacillus species is one of the most distributed soil contaminating bacteria and is linked to animals’ products quality. Three different grazing areas (Abo Al-Kasib, Al-Seba, and Al-Fawdistricts) in Basrah province were selected to determine the bacterial (Bacillus)distribution in soil. Samples were randomly collected from each field of the study. Based on the bacterial cell shape and staining (microscopy), colony morphology, biochemical tests, and the 16S rRNA genes amplification results, the bacterial isolates were identified to be from the genus Bacillus, of which Bacillus cereus was dominant in all samples. Five new isolates were identified based on 16S rRNA nucleotide sequences. Phylogenic analysis showed a close relation between three isolates to Bacillus cereuswith high 16S rRNA gene sequences similarity 99% (SAMU1), 99% (SAMU2) and 98.9% (SAMU3). Furthermore, two isolates showed sequences similarity to Bacillus paramycoides (99%, SAMU4) and Bacillus safensis (99%, SAMU5). All the above isolates were registered in the NCBI centre under the following names Bacillus cereus strain SAMU1, Bacillus cereus strain SAMU2,Bacillus cereus strain SAMU3,Bacillus paramycoidess strain SAMU4 and Bacillus safensis strain SAMU5 and were assigned the following accession numbers MK418732, MK490900, MK490901, MK490902,and MK490903respectively.In addition, some isolates were found to be 100% similar to the already identified Bacillus strains such as Bacillus safensis, B. pummilus, B. paramycoides and B. safensis. The current study investigated and mapped the distribution of Bacillus Spp in animals grazing areas that had been subjected to salinity increment.
529 Comparison of side effect between cyclosporine and tacrolimus as immunosuppressive therapy in Iraqi kidney transplant recipients, Mohammed Yousif Mahmood, Jawad Ibrahim Rasheed, Mustafa Rasool Hussein
Kidney transplantation now is the best treatment modality for end stage renal disease patients in comparison to other modalities and it offer the greatest potential for restoring a healthy, productive life. Advancing in immunosuppressive medications used during induction and maintenance phases and for treatment of acute rejection resulted in much success of kidney transplantation. Calcineurin inhibitors (CNIs), including cyclosporine and tacrolimus, are fungus-derived lipid-soluble small molecules that are the mainstays of current maintenance immunosuppressant. Considerable variability exists in their pharmacokinetics, interactions, and side effect profiles, which raises the question of which agent to use in the individual patient. Aim of study: To make a comparison in the side effect between cyclosporine and tacrolimus as immunosuppressive therapy in Iraqi kidney transplant recipients. Patients and methods: The current study is a retrospective study, which was conducted from kidney transplant recipients in Iraqi center of kidney disease and transplantation, that include the comparison of the side effect between two groups of recipients one receiving cyclosporine (n= 100) and the second receiving tacrolimus (n= 100), both combined with mycophenolate mofetil and prednisolone after one year and more of kidney transplantation. The cyclosporine dose was 3-5 mg/kg in divided dose and adjusted to maintain target whole blood trough level 100-200 ng/ml. Tacrolimus dose was 0.15-0.3 mg/kg in divided dose and adjusted to maintain target whole blood trough level 4-8 ng/ml. we collect the data from the files of recipients and compare between them. The data include demographic characteristic, renal function, serum calcium and phosphate, DEXA scan, blood sugar and post transplant weight gain, lipid profile, blood pressure, cosmetic side effects, infections, malignancies, gastrointestinal side effects, tremor and psychosis. Results: There was no significant difference between both groups in, serum creatinine [mean serum creatinine 1.01 mg/dl in tacrolimus versus 1.05 mg/dl in cyclosporine groups (p=0.3)], in gastrointestinal symptoms [(4.0%) in tacrolimus versus (3.0%) in cyclosporine groups (p=0.7)], in the incidence of psychosis [(0.0%) in tacrolimus versus (1.0%) in cyclosporine groups (p=0.3)], and in the incidence of bone disease. The incidence of hyperlipidemic was significantly higher in cyclosporine group [hypercholesteremia (29.0%) in cyclosporine versus (8.0%) in tacrolimus groups (p=0.001), hypertriglyceridemia (32.0%) in cyclosporine versus (18.0%) in tacrolimus groups (p=0.02), elevated LDL level (36.0%) in cyclosporine versus (19.0%) in tacrolimus groups (p=0.007)]. The incidence of post transplant hypertension was more significant in cyclosporine group [(28.0%) in cyclosporine versus (10.0%) in tacrolimus groups (p=0.001)]. The incidence of post transplant diabetes mellitus was more in tacrolimus group than cyclosporine group, but this difference was not statically significant [(19.0%)in tacrolimus versus (9.0%) in cyclosporine groups (p=0.07)]. The incidence of weight change was more significant in cyclosporine group [mean weight gain was 9.21 kg in tacrolimus versus 12.44 kg in cyclosporine groups (p=0.001)].The incidence of gingival hyperplasia and hirsutism was more significant in cyclosporine group [(5.0%) and (50.0%) respectively in cyclosporine versus (0.0%) and (0.0%) respectively in tacrolimus groups, (p=0.02), (p=0.001) respectively]. While the incidence of alopecia was significantly more in tacrolimus group [(19.0%) in tacrolimus versus (0.0%) in cyclosporine groups (p=0.001)]. The incidence of severe infection was more significant in cyclosporine group [(9.0%) in cyclosporine versus (2.0%) in tacrolimus groups (p=0.03). The incidence of malignancies were more significant in cyclosporine group [(4.0%) in cyclosporine versus (0.0%) in tacrolimus groups (p=0.04)]. The incidence of tremor was more significant in tacrolimus group [(40.0%) in tacrolimus versus (4.0%) in cyclosporine groups (p=0.001)]. Conclusion: Cyclosporine causes more cardiovascular side effect, more dyslipidemia and hypertension and more post transplant weight gain. Regarding cosmetic side effect cyclosporine found to cause more gingival hyperplasia and hirsutism, while tacrolimus cause more alopecia. Cyclosporine found to cause more incidences of severe infection and malignancies. The incidence of post transplant diabetes mellitus was more in tacrolimus but it was not significant.
530 Comparison of molecular subtypes between primary breast cancer and its metastatic auxiliary lymph node(s), Binan Adil Alaaragy, Iftikhar Altemimi, Yassir Alaa Shubbar, Kaswer Musa Alturaihi, Haider Rabee
Breast carcinoma primary tumors and synchronous axillaries lymph node(s) metastases may represent different malignant clones, although the daily clinical practice depends only on the molecular subtypes of primary tumor to select the adjuvant systemic treatment. In some instances the absence of primary tumor or presence of technical difficulties that prevent the identification of exact tumor subtypes in the primary mass mandate seeking for alternative to test. Aim: this paper aims to compare of molecular subtypes between primary tumor &metastatic lymph node(s). Method In this prospective cohort study, 50 patients with node positive breast carcinoma included. Immunohistochemical analysis by using Envision method for detection of biomarkers: ER, PR, Her2/neu and Ki-67 to determine the four molecular subtype then comparison between the primary tumor and its metastatic lymph node(s). Results: In this study there was 22 (44%) cases with luminal-A molecular subtype in both mass and lymph node, 21 (42%) cases with luminal-B in primary mass in comparison to 18 (36%) cases in their metastatic node, 3(6%) cases with Her2/neu enriched in the primary mass in comparison to 6(12%) cases in its metastatic node. There was 4 (8%) cases with triple negative molecular subtype which had no difference between them.. In Conclusions: There is no statistically significant difference between primary tumor and its metastatic lymph node(s) this concordance regarding the molecular subtypes may answer the question that pathologist can depends on the result of molecular subtypes on lymph node with good certainty if cannot do it on the primary tumor.
531 Tumor necrosis factor alpha (TNF-α) and interleukins (IL)-6 which was salivary cytokines serve as potential biomarkers and commonly used. TNF – α which was found in saliva more abundant compared to that in serum used as diagnostic utility in oral tumors Interleukin – 6 a multifunctional pleiotropic affecting many type of cells, it is a glycoprotein formed from 184 amino acid, Oral Benign Fibro osseous lesions, group of intraosseous disease of facial and cranial bones, define as replacement of normal bone by cellular ossifying fibrous tissues in spite of healthy type of bone. Aim: The study aims to evaluate of cytokines expression tumor necrosis factor alpha (TNF-α) andInterleukin-6 (IL-6) in the saliva of oral benign fibro- osseous tumors and healthy controls. Patients and methods: The study was involved sixty patients who were examined divided into two groups thirty patients (n=30) with oral benign fibro osseous lesions and thirty person (n=30) healthy control volunteers with good oral health. Results: revealed that the mean tumor necrosis factor (TNF-α) of the control group was 2.71 and standard deviation was 0.46 while the mean examined group was 2.95 and standard deviation was 0.42 which was statistically significant p value = 0.039. Our results revealed that the mean Interleukin-6 (IL-6) of control group was 15.96 and standard deviation was 2.49 while the mean examined group was 31.42 and standard deviation was 3.79 which was statistically highly significant p value = 0.0001 which was complained of benign oral fibro-osseous lesions. Conclusion: Salivary cytokines tumor necrosis factor (TNF-α) and the Interleukin-6 (IL-6) were high level in examined group. , Suha Mohammad Sami
Tumor necrosis factor alpha (TNF-α) and interleukins (IL)-6 which was salivary cytokines serve as potential biomarkers and commonly used. TNF – α which was found in saliva more abundant compared to that in serum used as diagnostic utility in oral tumors Interleukin – 6 a multifunctional pleiotropic affecting many type of cells, it is a glycoprotein formed from 184 amino acid, Oral Benign Fibro osseous lesions, group of intraosseous disease of facial and cranial bones, define as replacement of normal bone by cellular ossifying fibrous tissues in spite of healthy type of bone. Aim: The study aims to evaluate of cytokines expression tumor necrosis factor alpha (TNF-α) andInterleukin-6 (IL-6) in the saliva of oral benign fibro- osseous tumors and healthy controls. Patients and methods: The study was involved sixty patients who were examined divided into two groups thirty patients (n=30) with oral benign fibro osseous lesions and thirty person (n=30) healthy control volunteers with good oral health. Results: revealed that the mean tumor necrosis factor (TNF-α) of the control group was 2.71 and standard deviation was 0.46 while the mean examined group was 2.95 and standard deviation was 0.42 which was statistically significant p value = 0.039. Our results revealed that the mean Interleukin-6 (IL-6) of control group was 15.96 and standard deviation was 2.49 while the mean examined group was 31.42 and standard deviation was 3.79 which was statistically highly significant p value = 0.0001 which was complained of benign oral fibro-osseous lesions. Conclusion: Salivary cytokines tumor necrosis factor (TNF-α) and the Interleukin-6 (IL-6) were high level in examined group.
532 Preliminary assessment of dental practice risk during Covid-19 pandemic: a case-control study, Dunia Saad , Saad Khazal ALkazali , Saad Hikmat , Suha Mohammad Sami , Header Dakhel AL- Mualla
Objective: The aim of this study was to assess the risk of dental practice during Covid-19 pandemic. Method: This is a retrospective case- control study conducted in Iraq from 24 February to 10 April 2020, eligible case (693) patients and the control group (693) patients randomly. Results: Total number of the sample was 1386 (case 693, control 693 patients), the exposure (dental practice) were 7 (0.5%) patients, 3 (0.21%) of them were positive (+ve) while other 4 (0.288 %) were negative (–ve) result. Mean age for case group 42.4 years and for control group 38.7 years. For case group 418 (61%) males, 275(39%) females and for control group 402 (58%) males, 291 (42%) females. Odds ratio (OR )1.335 at confidence interval (CI) 95% (0.298-5.988) that mean possible associated risk is present but at this confidence interval it is statistically not significant. Clinical significance: The use of PPE with strict adherence to infection control measures can significantly minimize the risk of Covid-19 infection during dental practice.
533 The prevalence of Clopidogrel resistance in an Iraqi population, Ali A R Aldallal, Bassim I Mohammad, Ahmed N. Rjeeb, Salam Jasim mohammed, Khalid Amber
Background: Clopidogrel is a P2Y12 inhibitor indicated with aspirin to attenuate high platelet reactivity in patients who are at risk of undergoing thromboembolic events. Numerous investigations have prompted scanning and clinical tests to predict the resistance of patients to clopidogrel because not all patients respond to clopidogrel equally. Resistance to clopidogrel refers to a phenomenon by which clopidogrel fails to deliver a pharmacological target and may be measured by tests for platelet function. The best reasonable mechanism for the detected inter-individual variability in the inhibition of platelets leading to clopidogrel resistance is the differences in the magnitude of clopidogrel metabolism to its dynamic metabolite. Objective: This study aimed to investigate clopidogrel resistance in Iraqi patients who were residents in (Mid-Euphrates) governorates. Methods: A total of 324 patients were recruited into this cross-sectional study. All participants received a 600 mg loading dose of clopidogrel and scheduled for elective percutaneous coronary intervention (PCI). The platelet function test was conducted for all patients at least 2 h before PCI to determine clopidogrel resistance. Our samples were classified into the clopidogrel resistance group and non-clopidogrel resistance group. The present study examined clopidogrel resistance in an Iraqi population, and blood samples were obtained for the determination of clopidogrel resistance. The data from all participants by interview were recorded regarding factors that affect clopidogrel response such as age, weight (body mass index), diabetes, and medications used (e.g., amlodipine, omeprazole, and atorvastatin) in the form of a questionnaire. Results: The clopidogrel resistance rate in the Iraqi population was 34.25%. Several variables may augment clopidogrel resistance such as advanced age, body mass index, and omeprazole, while other variables such as gender, diabetes mellitus, amlodipine, and atorvastatin had no association with clopidogrel resistance. Conclusions: In this study, one of three patients belonging to an Iraqi population displayed clopidogrel resistance. Many variables contribute to clopidogrel resistance.
534 Assessment of Patients Satistification toward Primary Health Care Services in Al- Nasiriyah City, Munther K Al-Sadawy
Objectives: The study aims to: To assess patients satistification to ward primary health care service in Al- Nasiriyah city. Methodology: A descriptive study design was conducted though out the present study for period from 29 November 2018 to 1st march 2019. A purposive (non- probability) sample of 100 patients, study was done at three primary health care center in Al- Nasiriyah City. The questionnaire consisted of three parts: socio-demographic characteristics of the parents (age, gender, educational level, employment, marital status and monthly income).the second part consist of questionnaire consist of seven domains use for assessment of patients’ satistification toward primary health care service. The instrument's content validity was calculated by using panel experts and the instrument's internal accuracy was calculated by pilot analysis and Alpha Correlation Coefficient (r = 0.870) measurement. Data were analyzed using descriptive and inferential statistical methods, using version 20.0 of the Statistical Package for Social Science (IBM SPSS). Results: The results showed that the majority of the sample members range from 46 to 55 years of age. The terms of reference for the study sample are males, a greater number of education does not read and write and reflects (32.0%) the study., with regard to the social situation, the majority of the sample are married and represent 74 (74.0%) of the entire sample, the state of the profession, and the results indicate To be the highest proportion of the study. Most of the monthly income of respondents is insufficient and represents (49.0%). Tests indicate (pass). I put it in items (4, 5, 23, 24, and 25) and (failed) in all items. Conclusion: Many facets of primary care such as nursing, pharmacy and clerical services will also be included in this proposed research and their impact on patient satisfaction, not examined by the science.
535 Changes in Blood Biochemical Parameters in Bodybuilders Using Supplements, Steroids and their Effect on the Kidneys in Iraq, Ali A. H. Albakaa, Hasan Abd Ali Khudhair, Wisam R. N. Al-Muhsen
The enhanced body activities after taking the nutritional addendum by athletes were observed via fluctuation in the clinical biomarkers. There are some previous evidences recorded that the nutritional supplements have a benefit and side influences, hence current paper was aimed to determine the potential influences of nutritional support compounds on biochemical parameters and kidneys damage among bodybuilders people. Two subjects of sportsmen bodybuilders; first group not take the nutritional supplements (control group), whereas the second group take the nutritional supplements (users group) were included within current study and screened for their serological level of creatinine, urea, total protein and albumin with measuring urine total protein and pH. The results showed that the mean titer of serum creatinine, serum albumin and urine total protein were significantly higher among users group compared to control group, whereas the level of serum urea, serum total protein and urine pH revealed non-significant different between both groups. For subjects aware to the risks and side effects of taking the nutritional supplements, the users group exhibited a significant higher frequency % of unaware to the risks in comparison to control group. In denouement, there is prodromal defect in the kidneys of the nutritional supplements consumers, confirmed by serum creatinine, serum albumin and urine total protein increase. Many of nutritional supplements users not have enough information about the consequences and side effects of the decompounds hence exercising related with abuse of it without consulting a dietitian may be hazardous.
536 Determination of Pregnant women Knowledge toward Risk Factors of Vitamin D Deficiency and Measuring Level During Pregnancy in Al- Nasiriyah City, Ali Abdulhakeem Hassan Albakaa , Amer Muhssen Naser , Ali Riyad Saleim
Objectives: The aims of this research are three-fold. First, an examination of a pregnant female’s information on risks associated with deficiency in vitamin D in pregnancy at the Al-Nasiriyah primary health clinic, second, an evaluation of levels of vitamin D through various times in pregnancy, third, to identify if an association exists between levels of vitamin D in pregnancy and the trimester of pregnancy. Methodology: The following study was run from 27th December 2018 to the 1st of April 2019. A descriptive study design was adopted including 100 pregnant females who were selected using purposive (non-probability) sampling. The study location was Al-Nasraniyah; specifically, the Al-Karar primary health care clinic. Data collection was completed through a self-administrative report and a questionnaire that was split into three sections. The first is the socio-demographic characteristics of the parents including their age, job, income (monthly) and education. The second section included the pregnant female’s medical data including gestational age, gravida, para, and exposure to daily sunlight, numbers of abortions and whether they consume vitamin D or multi-vitamin supplements. The final section consisted of ten questions investigating the pregnant female’s knowledge of the risk factors associated with vitamin deficiency in pregnant females. Quantification of all participants’ vitamin D serum levels was also conducted. Validity testing was performed using both external and internal means. For external validity a panel of experts was employed, while internal validity was established using a pilot study and the alpha correlation coefficient at r=0.870. The Statistical Package for Social Science (IBM SPSS) version 20.0 was used for statistical analysis of the data using both descriptive and inferential methodologies. Results: the study results indicate that the majority that (50.0 %) of the patients within age group (15-25) .Regarding to the level of education, the greater number of them do not read and write and they are accounted for (32.0%) of the sample. In regarding to pregnant women medical sheet information the majority of study sample gestational age found at second semester with percent (56 %) of all study sample, the daily sun light exposure among study sample there are higher percent with less than one hour per day which account for (67%) of study sample.patients have knowledge toward risk factors of Vit D deficiency only at presented as ( pass) in ( 3 and 6) items and presented (failed) in all items ,the high prevalence of Vit D deficiency among study sample with percentage ( 42%) of all which are suffering from Deficiency of Vit D during their pregnancy period . the study results show there is not statistically significant differences Between Vitamin D Level during Pregnancy and first Semester and statistically significant differences Between Vitamin D Level at Semester of Pregnancy by P value < 0.05, While there is highly statistically significant difference Between Vitamin D Level during Pregnancy and Third] Semester of Pregnancy by P value < 0.01. Recommendations: This study proposes the provision of pregnant females with an educational session/s to educate them on the risks associated with deficiency of vitamin D in pregnancy.
537 Recalcification of Carious Lesion with Aids of Calcium Oxide Nanoparticles, Bushra Habeeb Al-Maula, Suhad Jabbar Al-Nasrawi, Alaa Dakhil Yassir, Abtesam Imhemed Aljdaimi, Ruba Mansour Mustafa, Saleh Zidan
Application of CaO nanoparticles has been stated to have a promising effect in the increasing of the resistance of mineralized tissue against cryogenic challenge. This project aimed to investigate the recalcifying efficacy CaO nanoparticles applied to the natural lesion. Thirty enamel samples were prepared from premolar teeth. They were divided into two main groups of three subgroups in each (Sub-G1, Sub-G2, and Sub-G3), one group from the affected area and another from the adjacent sound area. The samples in Sub-G1 were coated with the bonding agent, in Sub-G2 were coated with CaO nanoparticles loaded bonding agent, and Su-G3 with no coating (control). After storage in artificial saliva, the mineral contents were quantified with aids of scanning electron microscope fitted with energy dispersive spectroscopy (SEM-EDX). The statistical analysis displayed an increase in the Ca surface contents of carious and sound samples in Sub-G2. This indicates that coating the enamel with CaO nanoparticles loaded bonding agent could enhance the remineralization of naturally demineralized hard tissue and provide protection for the sound tissue.
538 Prevalence and Types of Husband Violence against Women in AL Najaf AL Ashraf city, Iraq, Huda Ghazi Hameed, Nabaa Ali Abd Al Hassan
Background: Violence against women is a worldwide problem and intimate partner violence is the commonest form. It occurs in all societies, cultures and different religions. Objectives: To measure the prevalence, types and consequences of husband violence among a sample of women attending primary health care centers in AL-Najaf city. Methods and Materials: A cross-sectional descriptive study done on a sample of 323 women randomly selected from primary health care centers in AL-Najaf AL-Ashraf city beginning from 1st of April 2018 to 1st of February 2019. Data were collected using a modified version of the World Health Organization’s questionnaire for the domestic violence. Before the data collection, the official agreements were taken from the general health directorate and the managers of primary health centers, and verbal consent from the participants, the questionnaire containing information about demographic factors and questions about types and frequency of the violence during whole life and the last 12 months. The information was analyzed using SPSS version 20. Results: The prevalence of lifetime intimate partner violence against women was 58.5% and the emotional violence is the commonest type of violence followed by physical and sexual violence (57%, 41.2% and 19.8% respectively). The prevalence of past -year violence was 45.6% also the emotional violence was the commonest form 43% followed by physical 25.2% and sexual violence 10.1%. Conclusions: More than half of the sample was victims of violence regardless of its type with the emotional violence being the commonest type of violence.
539 Influence of High resolution computed tomography (HRCT) in reviewing the diagnosis of chronic obstructive pulmonary disease (COPD): A study of 16 male patients, Ammar Jabbar Majeed , Falah Abdulhasan Deli , Samet Elias Kasim
Background: Chronic obstructive pulmonary disease (COPD) is manifested mainly as two main clinical phenotypes chronic bronchitis and emphysema. It is characterized by the presence of poorly reversible airflow obstruction; the presence of other histopathological changes may contribute to the poor response to treatment in many patients. Aim of study: To detect additional changes that involves the lung tissue such as interstitial lung fibrosis in COPD patient. Patients and Methods: This cross-sectional study was carried out on (16) male patients diagnosed with COPD. All patients were during the stable state, and investigated by spirometry and HRCT. Results: Eight patients had emphysema, four had emphysema and lung fibrosis, two had traction bronchiectasis, one with apical fibrosis and another with possible lung neoplasm, four patients (out of seven) who had obstructive and restrictive pattern on spirometry had interstitial changes compared to two (out of nine of those with obstructive pattern only. Conclusions and Recommendations: HRCT scan is an important diagnostic tool to detect other pulmonary changes that can occur in patients with COPD. Larger studies are needed and obtaining histopathological assessment for these patients.
540 Seminal plasma IL-1and IL-18 were independent on expression of their related genes in cellular components Iraqi infertile men, Rehab Sh. AL-Maliki , Kareem G. Mohamed , Haider F. Ghazi *
Background: The NLRP3 inflammasome protein complexes are the intracellular receptor responsible for expression NLRP3 and pro-IL-1 and maturation of IL-1 and IL-18 by activation of caspase-1. It has been widely accepted that these cytokines are related to infection and inflammation as well as have impaired effects on sperm quality in patient with spinal cord injury. Objective: the aim of the current study was to determinate if the NLRP3 inflammasome expression and activation and their end products (IL-1and IL-18) have negative impact on the sperm quality in infertile men. Methods: semen samples were obtained from 264 infertile men by masturbation after 3-5 days of abstinence; these samples were subjected to semen analysis to evaluate sperm motility, morphology and count. Total mRNA of NLRP3, IL-1and IL-18 was extracted from whole semen cells pellet and their expression was done by using qRT- PCR after convert them to cDNA. Quantitation of both IL-1β and IL-18 in the aliquots of seminal plasma was performed using enzyme-linked immunosorbent assay (ELISA). Detection of NLRP3 and caspase-3 in semen smear was done using Immunoperoxidase staining kit. Results: there were significantly weak positive correlation between the concentration of mRNA of IL-1and viscosity (P=0.035). Between the concentration of mRNA of NLRP3 and sperm count and non-progressive motile sperms (P=0.023) (P=0.013(respectively. There were significant weak negative correlation between the amount of mRNA of IL-1and progressive motile sperms (P=0.037). The amount of NLRP3 protein was significantly positive correlated with non-progressive motile sperm (P=0.014). WhileNLRP3 protein was significantly weak negative correlated with round cells (P=0.019). Translated protein of caspase-3 in the sperm cells was highly significant weak negative correlated with round cells (P=0.003). Conclusion: the present study suggests that the concentration of NLRP3 expression and its translated protein in defective sperm have indirect impaired effect on sperm parameters especially sperm motility and sperm count.
541 Low Vitamin D Level and Its Relation to Cognitive Function in Chronic Kidney Diseases (Dialysis and Non-Dialysis) Patients, Alaa Abdlhussein Abdulzahra, Ali Jasim Hasim Al Saedi
Cognitive disability and vitamin D deficiency are mostly common in chronic kidney disease (CKD) patients. Vitamin D applies protective and controlling role in the CNS. Low level of D vitamin has been related with the weakness of muscle and bone trouble, circulatory disorders (hyperlipidemic, diabetes, and hypertension), oxidative injury, infections, immune decline and neurocognitive impedance. The level of vitamin D that is suboptimal is very common as well as to CKD. The two disorders, especially predominant in patients older than 65 years, and are known dangers for firmly connected with cognitive impairment. In spite of the fact that it is estimated that patients with CKD insufficient vitamin D level might encounter a quickened cognitive decline, and just little effectively planned studies achieved in this subject. Aim of study: Assessment of cognitive function in association with vitamin D level in patients with CKD with or without dialysis. Patients and methods: the study include two groups; 61 dialysis and 56 CKD nondialysis patients enrolled in our study, in which CKD was defined as eGFR
542 Filipino Nursing Students' eHealth Literacy and Criteria Used for Selection of Health Websites, Ryan Michael F. Oducado, Rome B. Moralista
Possessing eHealth literacy skills among students has been increasingly appreciated in nursing education. Despite eHealth literacy has been widely studied, little published research is available regarding Filipino nursing students’ eHealth literacy. This study aimed to determine the self-reported eHealth literacy of Filipino nursing students and the relative importance of the criteria they use when selecting health websites. A cross-sectional research design was used involving 66 conveniently chosen nursing students. Self-reported data were collected using adopted questionnaires. Data were analyzed using descriptive statistics, the Mann-Whitney U test, and Spearman’s rho. Findings revealed that nursing students had high eHealth literacy. The utility of information and accuracy of information were the top criteria students consider important when selecting health websites. There were no significant differences in the eHealth literacy of male and female and first-year and second-year nursing students. The extent of Internet use for health information, perceived usefulness of the Internet when making health decisions, and perceived importance to access health resources on the Internet were significantly correlated with eHealth literacy. To derive full benefit from online health information, nursing students’ literacy needs in the Web context and digital environment must be given attention
543 Knowledge of medical students regards antibiotic consumption for upper respiratory tract infections in Basra, Maysaloon A. AL-Sadoon
Upper respiratory tract diseases (URTD) are categorized as illnessescarriedserious sickness connecting the upper respiratory tract, for example the nose, sinuses, pharynx as well as larynx. Thisstudy was attempted to assess the data, frames of mind and practices of Iraqi clinical understudies towards the utilization of anti-microbial agents in the treatment of URTD, across sectional investigation questionnaire design assumed to investigate the utilization of anti-microbial agents among (209)student in University of Basra college of Medicine.Most of respondents demonstrated that they had past information of antibiotic agents 99% ought to be utilized in the treatment of bacterial diseases, 3.3% believed that they may likewise be utilized in the treatment of viral contagions. In Three themes of learning (advantages, dangers and utilization of anti-microbial agents), a majority of participants had high level of awareness on the benefits of antibiotics prevent the complications of colds (89.5%). while, a minority of them had adequate knowledge on the benefits of using antibiotics, such as antibiotics could not shorten the length of colds (19.1%). However, 95.7% of all subjects surveyed had proper understanding of the misuse of antibiotics that could lead to drug resistance.
544 Study the Impact of the environmental behavior on educational level of students in College of Health and Medical Technology –Baghdad, Iraq, Maytham Salim Al-Nasrawii, Mohammad Abdul-Baqi Abdul-Mohsin
Background: A student is a learner, or anyone who attends an academic institution. Student is often used for anyone learning, including early-career adults taking vocational education or returning to college. However, the existence in most countries of noticeable differences in academic achievement in relation to poor educational standards garnered attention in this area. School academic achievement is not only linked to school-related factors, but also to the socio-economic environment in which students are educated.Over this, westudy the scientific, socialand psychological status of the students in the Health and Medical Technology College in Baghdad and to finding out any association between this variable with the demographical characteristics and socioeconomic status of the study samples. Subjects&Methods:A descriptive cross-sectional study was carried out to evaluate the scientific and psychosocial status of 200 students involved. selected randomly from the " Health and Medical Technology College in Baghdad". Demographic characteristics and socio-economic status of the study sample were obtained.Using SPSS (Statistical Process for Social Sciences), data were analyzed. Review of the descriptive details, including: Analysis statistics tables ((Score Mean (M.S.), Standard Deviation (S.D.), Relative Sufficiency Percentage (R.S. percent) and Score Cut-off (0.5) according to score 0,1)); Grand mean of score (G.M.S), Person's Correlation Coefficients, and Using graphical presentation (bar charts and cluster bar charts). Analysis of inferential statistics, included binomial test, Chi-Square test (χ2), testing the person's correlation coefficients, and testing the contingency coefficient. Result:In total of all participants, those completed the questionnaire, more than half of the respondents 113 (56.5%) were male, 144 (72%) atage group (20- 24) years. More than half of participates 105(52.5%) had moderate Socio-Economic Status.The study sample has failure on the scientific domain assessment while they had positive (pass) in social and psychological domain,the results reveals' that main overall assessment domains (scientific, social and psychological status) had a significant different with age groups and grades, gender and marital status, while no-significant different with socio-economic. Conclusion: There was positive impact of the environmental behavior on the educational level of students in College of Health and Medical Technology –Baghdad.
545 Effect of Citrullus colocynthis Medium on in vitro Oocytes Maturation Rate, Nabaa A. Al-Nawab*, Ibtesam R.T. Al-Delfi, Rusul Muzher Hussein
The traditional IVF cycles involve ovarian stimulation protocols. Hormones treatment may increase the rate ofincidence of Ovarian Hyper-stimulation syndrome (OHSS). In vitro maturation (IVM) of oocytes can be the alternative method. Therefore, this study aimed to investigate the influence of Citrullus colocynthis (C. colocynthis) extract on improving the in vitro maturation rate of oocytes. The result of this study showed that there was an improvement in oocytes maturation after one day treatment. Data showed a significant increases in the percentage of oocytes maturation when they treated with C. Colocynthis extract compared with control group P
546 Association between the level of Bradykinin and viral infection in patient suffering from respiratory infection, renal transplant, and renal failure, Laith A.I.K. Al-Kaif1*, Hayder A.A. Al-Asadi,Younis A.K. Al-Khafaji, Raheem T. Al-Mammori, Hussein A. Mezher, Waleed M. Kazem, Mustafa W. Kazem, Hussein A. Majali,Ali H. Ali1, Abbas Q. Obaid,Jasem M. Hussein,Bassam M. Atyaee, and Rusul H. Edin
Background & Objectives: The study was conducted to find out the relationship between viral infection and bradykinin level in sera of human suffering from virally infected, respiratory, renal transplant or renal failure diseases. Therefore, this study includes the evaluation of the immune status in the sera of patient suffering from renal failure, renal transplant and respiratory infected whose were virally infected with healthy subjects. Methods: The immune response was evaluated by means of ELISA test for bradykinin level in the sera of 80 specimens included in this study: eight renal transplant, twenty renal failure, thirty-seven respiratory infected, and fifteen healthy subjects’ that were collected during the period extending from October 2018 to March 2019. Data about individuals were collected aseptically in sterile containers, after getting all data in special formula including, name, gender, age, disease. Results: The results of the present study showed that there were very high significant difference of bradykinin level in sera of patients with a healthy group at p
547 Prevalence of Pantoea spp. among recurrent UTI patients with emphasis on risk factors and antibiotic resistance patterns in Al-Qadisiyah hospitals, Iraq, Suaad A. Fazaa, Mayyada F. Darweesh
Background: Pantoea spp. is plant bacteria then regard as opportunistic pathogen in human associated with outbreak and several clinical infection so consider as a "mysterious bacterium of evil and good". Objectives: The aim of the current study is to investigate the important risk factor that associated Pantoea infections with recurrent UTI patients identify the bacterial causes and antibiotic susceptibility profile to decrease the reinfection and prevent complication of recurrent UTI. Material and Methods: A retrospective- study was performed from December 2018 – July 2019. A total of 800 patients with recurrent urinary tract infection attending to AL-Qadisiyah hospitals in both sex and different age were studied. Specimens were cultured on specific media, and then bacterial isolates were identified via morphological, biochemical and Vitek-2 systems with antibiotic profiles after received agreement from patients and ethical comments from hospitals. Results: The results show 55 isolates of Pantoeaspp were isolated. In majority of patients, Pantoea agglomerans was isolate in (56.36%), Pantoea annanatis(21.81%), Pantoea calida (12.72%),Pantoea stewartii(9.09%), the study revealed that long-time catheterization and V.U.R(vesico-uretral reflux) almost equally contributed (29.09%) and (21.81%).Antibiotic resistance profile confirmed that all Pantoea isolates are multidrug resistance, (89.09%)resistance toward ticarcilin & Clavulanic acid, and show variable resistance toward β-lactam and aminoglycosides. The Levofloxacin and Netimicine consider drug of choice in treatment. Conclusion: The chances of urinary tract infection increase in the presence of risk factors also, accurate susceptibility pattern increase the success of therapy and prevent complication of disease.Pantoeasppregard as serious opportunistic pathogen has ability to infectimmunocompromised patients and causes aggressive disease as a result of multidrug resistance ability.
548 Activity of Zinc Oral Dispersible Tablet on Marjory Clinical Type of Recurrent Aphthous Stomatitis Ulceration, a Clinical Trial Human study, Ghadah Ali Al-Oudah , Ameer Hamdi AL-Ameedee, Sinan Abdul-Sattar Shwailiya
Background:Oral recurrent aphthous stomatitis is the most common disease of the oral cavity. The underlying etiology remains unclear, and no curative treatment is available. Zinc is essential and useful for normal growth and tissue repair, zinc acts as an integral part of several enzymes important to protein and carbohydrate metabolism. Objectives:The presentstudy aimedto use systemic drug used zinc oral dispersible tablet (20mg).Illnesses related to the oral cavity by studyingits effects in oral recurrent aphthous stomatitis major clinical type (ORAS), this systemic therapy is not indicated in such situations among other drugs. Methods:In this study patientspresented with (ORAS) ulceration lesions were treated with zinc oral dispersible tablet (20mg) administered orally once daily after meal. The dispersible tablet was administered orally once daily for 14 days, 52 patients (36 males and 16 females aged between 28-30)with biopsy-confirmed aphthous ulceration of the lesions area, divided into two groups;group A, 28 patients were randomly assigned to receive zinc oral dispersible tablet, 20 mg/dayonce per day, and group B;24 patients with oral placebo daily for 14 days. Results: The results showed that administering of zinc oral dispersible tablet once per day accelerated the healing process within a short time period (8 days) without complications or disfigurement in all patients.Group A, 22 patients, (healing rate 0.66%) of 28 patients were used zinc oral dispersible tablet (20mg) doses administered orally had complete healing of aphthous ulcers at period time eight days of clinical investigation evaluation and weight increase rate by 0.32% kg during the time period of the study, compared with group B, only8 patients (healing rate 0.21%) of 24 placebo-randomized patients eating ability caused by oral cavity aphthous ulceration were improved markedly and had a weight loss rate by 0.53% kg. Conclusion:In this study showed that, the zinc oral dispersible tablet treatment was effective in healing of the major type aphthous ulceration and the end-points of the study were complete healing and absence of any discomfortpain while eating within a short period of treatment.
549 Occupational safety and working conditions provided by the Municipality of the capital for waste removal workers: study in one of the municipal departments, Nibras Mohammed A. Alsaffar and Nagham Hussein Neama
The study which was carried out in the cleanliness department of the Karrada municipality department, related to the workers of waste removal, showed that there is a lack of awareness on the part of waste workers towards the occupational health and safety equipment that serves them for the purpose of maintaining health and protecting them from work hazards, such as gloves, occupational safety shoes, head cover and rain suits. In the winter and other equipment and showed the lack of several first aid in the wheels of lifting waste and the lack of periodic checks for workers from infectious diseases and heavy elements and that most workers work daily wages and few of them on a temporary contract, as well as between the study Not to participate in training courses in the field of waste lifting and occupational health and safety The study showed the interest of officials to maintain the health and safety of workers and achieve their satisfaction and the adoption of complaints submitted by them and there are amounts calculated on the basis of the wages of lifting waste and cleaning sewage and written in the lists of wages of pure water, but spend on other things.
550 Hematological impact in mice due to alteration in their nutritional style, Yusur Falah Faraj, Shatha Salah Asad, Jamela Jouda, Khalid M Salih, and Ali Sameer Abudlghani Altaee
It is well established that alteration of diet style from plant-based to fat-rich diet lead to many complications including cardiovascular disease, metabolic syndrome, osteoporosis, and as well as anemia. According to our previous researches, changing in nutritional style from a conventional diet to fat-rich diet caused disturbance in the histology and function of different organs. So this work aims to study the effect of transition from a completely plant-based diet to 10% animal products on different hematology parameters in mice by using two groups of adult male Balb/c mice (n=8 mice for each). The first group represents restricted group that fed on conventional diet containing 10% sheep brain homogenate, while the second group represents control group that fed ad libitum on conventional diet only. The mice were sacrificed by cervical dislocation after 7 days. Blood samples were collected from the eyes in sterile tubes and immediately used to determine complete blood count (CBC). The results showed that platelets count, platelets/Lymphocytes ratio, and the total count of white blood cells particularly monocytes in restricted group are significantly higher than those in control group. However, Red blood cells RBCs, hemoglobin concentration, hematocrit, RDW-SD level are significantly lower in restricted group in comparison with those of control group. In conclusion, these results indicate that nutritional style alteration can affect cellular elements of blood and may provoke anemia, thromboembolism and immune response which need further investigation.
551 Negative effect of therapeutic, double and overdoses of cefixime on the liver and kidneys of male albino rats, Shatha Hazem Shaker, Qasim Azeez Razooqi, and Rashid Khamees ShabaN
This study was designed to investigate the negative effect of double and excessive doses on the treatment of cefixime on male rats. 20 animals were selected with weights ranging from 190-200 mg and were in good health. These animals underwent good ventilation and appropriate laboratory conditions. The animals were divided into four groups, the first group was drawn with distilled water and the control group was returned, the remaining three groups were dosed using cefixime as a therapeutic dose, double dose, and overdose, respectively. After the 21-day dose, the results showed that tissue damage occurred in the liver tissue and significantly increased its enzymes in dosing groups with cefixime. Also, tissue damage in the kidneys with a significant decrease in the level of urea and a significant increase in the creatinine level of cefixime doses compared with the control group.
552 Effect of Different Concentrations of Tramadol on testes and male hormones in Young and Adult Mice, Assal G. Alshammary, Rafal N. Abbas, Jamela Jouda, and Mahdi S. Jumaa
This work aims to study the pathophysiological and physiological toxicity effect of tramadol in different concentrations, equivalent to less than 400mg which thought it is safe, on testes and male hormones. Forty-eight male Balb/c mice were used in this work and divided into two age groups; an adult group aged 8-9 weeks and young group aged 4-5 weeks. Every age group was divided into three subgroups (n=8mice). The first group consumed a high concentration of TM, 40mg TM/ kg bodyweight daily which equivalent about 421.2 mg TM/65kg bodyweight. The second group was consumed a low concentration of TM, 20 mg/65kg daily. The last group consumed only water ad libtum and served as a control group. After one month, the mice were sacrificed. Blood samples were collected and used to determine testes function and concentration of male hormones. Testes were collected and put in 10% formalin for path histological study. Testes of mice consume TM shows many histopathological changes compared to control. Significantly higher FSH, LH, and prolactin and lower testosterone levels in the mice group consumed tramadol compared to control groups. These changes is higher in the mice consume high TM concentration. As conclusion, taking tramadol in long term will have severe effects of testicular tissue which could cause male infertility even if it has given dose is less than 400mg daily which urges attention and not to use this type of drugs for long periods, whatever the reasons and looking for healthy alternatives to it.
553 The citrulline test as an alternative for arginine in stimulating growth hormone, Raheem S. Jebur, lecturer Dr. Rawaa H.Ali and Dr. Mohammed Sh.Alawady2
OBJECTIVE: children attending the specialized centre for endocrinology and diabetes and the children hospital in medical city who suffer from short stature. SUBJECTS AND METHODES: human Growth hormone (GH) is a hormone that is essential for normal growth and development in children. It promotes proper linear bone growth from birth through puberty. There are many methods for stimulation growth hormone, the major method is the by glucagon IM and arginine infusions. This test is very important for assessment pituitary gland in children and adult for the produce and secretion growth hormone. 4o child suffer short stature from attending the specialized centre for endocrinology and diabetes and the children hospital in medical city. Citrulline it is amino acid that is used today in medicine as a cure for hypotonic in adult and blood pressure reduce in children. Citrulline converts to arginine in the urea cycle and body. in this research used citrulline for the stimulation growth hormone alternative for arginine children and camper with stimulation by glucagon. SETTING: This study was performed during November 2019to February 2020.subject were selected from among the were diagnosed by the growth scale and to know the value of the growth hormone. When reviewing, the child must be fasting the basil blood is drawn and then the child will be injection by glucagon 0.03 mg\kg and then after 2 hours, the second withdrawal is done to see the extent of stimulation. After a period of two weeks to a month, the process is repeated for the same child using the citrulline oral at 0.33 gm\kg. And measured the s. glucose, s. creatinine, blood pressure and BMI. Aim of this study: Test the citrulline as a substitute for arginine in a growth hormone stimulation test and find out how efficient it is with stimulation compered to glucagon. RESULT: The results showed for 40 children that the highest rate of stimulation with glucagon is 5.662 ng\ml from 0.965 ng\ml as a basil and the highest stimulating rate with citrulline is 3.258 ng\ml from1.036 ng\ml as a basil, which means that there is a significant difference between the two cases and that the glucagon Best as a catalyst of citrulline. The ratio of the efficiency of the citrulline compared to the glucagon is 57.7%. CONCIUSTION: This study has been proven the citrulline can be used as stimulation for growth hormone. With the need for other research to know the optimal dose and appropriate time for the highest stimulation
554 Effect of adding guar gum to yogurt free fat and studying some of its properties, Shaymaa Rifaat Khairi, Shaymaa Saady Lafta, and Ebtisam F. Mousa
The dairy industry is in dire need to master the means of developing low-fat or low-fat dairy products in order to meet the requirements of the consumer. Such products are not easy due to the role played by fats in improving the texture, texture and taste of dairy products called fat alternatives The low-fat low-energy produced yoghurt from a sorting milk with the fat substitute of gum Arabic (GUAR GUM) with three levels of 0.1, 0.2 and 0.3% in addition to the positive control experiment in which the yogurt was made from whole milk and the negative control experiment in which the yogurt was made from Milk sorted without any addition. The best results to improve additives by determining the rheological tests, which included viscosity examination , clarity of automatic whey , water retention and the excelled treatment was yogurt with 0.2% guar gum (The treatment A2). The rolls above are very close to the treatment of positive control and outperformed the treatment of negative control. The results of sensory evaluation showed that the treatment (A2) was also the best.
555 Molecular detection and antimicrobial activity of Endophytic fungi isolated from a medical plant Rosmarinus officinalis, Shimal Yonuis Abdulhadi, Ghazwan Qasim Hasan and Raghad Nawaf Gergees
Endophytes are tiny organisms present in living tissues of distinct plants and have been extensively studied for their endophytic microbial complement. Roots of Rosmarinus officinalis were subjected to the isolation of endophytic fungi and screened for antimicrobial activity against Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae) bacteria. Genomic DNA from active fungal strain of Trichoderma harzia was isolated, and the internal transcribed spacer (ITS) region was amplified using ITS4 and ITS5 primers and sequenced for genetic inference in fungus. The crude extract of T. harzia isolate with Ethyl acetate was showed significant antimicrobial activity against P. aeruginosa, S. aureus, K. pneumonia, B. subtilis and E. coli. The antimicrobial activity was highest against P. aeruginosa at concentration of 40µg/ml, followed by S. aureus and K. pneumonia at the same concentration. The lowest antimicrobial activity was against by S. aureus at concentration of 60µg/ml. The current study is confirmed that the antimicrobial activity is due to bioactive compounds founded in endophytic fungi.
556 Evolution of some Biochemical and Hematological Parameters of Thalassemia Patients in Maysan Governorate, Iraq, Nidhal Abdullah Hashim, Younus Jasim Abdullah, Hasan A.Ibadi
Thalassemia is inherited anemia publicity that common in the world and especially in Mediterranean region, and the most important cause of mortality in patients. The study included (108) patients (males & females) with thalassemia in Amara city and (20) healthy individuals as a control group. All subjected to examination tests for assessment hematological parameters as well as liver functions tests.The results showedasignificant increase(p
557 Intestinal Helminth Parasites of Caspian Turtle Mauremys Caspica (Gmelin, 1774) (Testudines, Geoemydidae) From Al-Diwaniya Province, Iraq, Habeeb W. Kadhum Shubber, Ali B. Mohsen Alwaaly, and Mohammad K. Mohammad
The caspian turtle Mauremys caspica (Gmelin, 1774) is a medium-sized semi aquatic freshwater turtle. It is widely distributed in suitable areas throughout Iraq. A total of 28 road-killed specimens have been examined for their intestinal helminthic parasites. Results have been showed that 78.6% of this turtle were infected with one or more of following parasite species of Telorchis assula (Trematoda), Falcaustra araxiana, Serpinema microcephalus, and Contracaecum sp. (Nematoda).The statistical analysis showed that there were significant differences in prevalence between male and female turtles in parasitic infections (P
558 Organizational Behavior and Its Relationship to the Leadership Personality of the University Student, Assistant Professor Dr. Sufyan Saeb Al_Madhidi
The relationship between organizational behavior and the leadership personality is a positive function for males and females and for the benefit of males, as the leadership personality carried by males leads intuitively to organizational behavior. The relationship between organizational behavior and leadership personality is a positive function of scientific and human specialization and in favor of scientific specialization, as the time regulation of scientific disciplines and the organization of the schedule of daily life is always more organized for students of scientific colleges at the expense of students of human colleges. We recommend attention to building the organizational behavior of the individual since the first stages of study, build a character who can take responsibility from childhood. Preparing students who are able to build the leadership personality in a way that enables them to lead the groups they belong to in the classroom or the school stage and establishing positions and information in the curricula that introduce the student to the importance of organizational behavior and leadership personality.
559 Convalescent Plasma Therapy for Pandemics, COVID-19 as example: A Mini Review, Abubakar Yaro, Phiri N, Umar AbdulkadirNda
Pandemics are now becoming more frequently due to change due to human beings change of lifestyle. COVID-19 is a typical example of the global burden faced by humanity. During the pandemics, there is the urgent need of effective therapeutic intervention. In COVID-19, there is no specific drug, although remdesivir in combination with anti-inflammatory agents have been shown to shorten hospital stay. A novel approach is the use of convalescent plasma as therapy. This mini-review discusses the historical aspect of the use of CP as therapy, evidence from data on utilization of CP in COVID-19, acquiring CP and plasma composition, patients recruitments, risk of CP therapy, and potential mechanism of action. The review concludes that CP therapy would be a novel approach in managing pandemic when urgent therapeutic interventions are required. However, more clinical data is required to for definite conclusion on it use.
560 Antimicrobial Resistance Patterns and Beta-lactamases Producing of Carbapenem Resistant Klebsiella pneumonia Isolated from Iraqi Patients, Mariam Hadi Mohammad, Mahmoud Ibrahim Ismail
In this study, a total of 381clinical samples were collected from five hospitals in Baghdadduring the period July2018–January2019. The samples were cultured on MacConkey agarand blood agar plates to isolate Klebsiella Pneumonia in order to estimate its prevalence rate antimicrobial resistance patterns and Beta-lactamases produced. Outof 381 samples, 342 (89.8%) had Klebsiella pneumoniae. Thirty eight of342 of K .pneumoniaeisolateswere resistant to carbapenem (CRKP).A phenotypic detection of beta-lactamases showed that allCRKP isolatesdid not produceextended spectrum beta-lactamases.Whereas all these 38 isolates were produced metallo-betalactamases (MBLs).Regarding to antibiotic susceptibility test, these 38 CRKP isolates were resistant to 13of15antibioticsused in this study.Whereas 25 out of 38 CRKP isolates were sensitive to Levofloxacin. According to MIC values, the metallo-betalactamase producing CRKP isolates were resistance to 11 out of 15 antibiotics used. However,13 out of 38 CRKP isolates were resistant to levofloxacin.
561 Antimicrobial Resistance Patterns and Beta-lactamases Producing of Carbapenem Resistant Klebsiella pneumonia Isolated from Iraqi Patients, Mariam Hadi Mohammad, Mahmoud Ibrahim Ismail
In this study, a total of 381clinical samples were collected from five hospitals in Baghdadduring the period July2018–January2019. The samples were cultured on MacConkey agarand blood agar plates to isolate Klebsiella Pneumonia in order to estimate its prevalence rate antimicrobial resistance patterns and Beta-lactamases produced. Outof 381 samples, 342 (89.8%) had Klebsiella pneumoniae. Thirty eight of342 of K .pneumoniaeisolateswere resistant to carbapenem (CRKP).A phenotypic detection of beta-lactamases showed that allCRKP isolatesdid not produceextended spectrum beta-lactamases.Whereas all these 38 isolates were produced metallo-betalactamases (MBLs).Regarding to antibiotic susceptibility test, these 38 CRKP isolates were resistant to 13of15antibioticsused in this study.Whereas 25 out of 38 CRKP isolates were sensitive to Levofloxacin. According to MIC values, the metallo-betalactamase producing CRKP isolates were resistance to 11 out of 15 antibiotics used. However,13 out of 38 CRKP isolates were resistant to levofloxacin.
562 Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq, Israa Husam Mohammed Al-Hasheme, Thikra Abed-Aoun Hassan Al-Tammime
The current study aimed at finding the relationship between infection of intestinal parasites and some hematological (complete blood count) and immunological parameters (histamine, interleukin-5, and IgE) in children visiting and infertile at Karbala Children's Hospital in Kerbala, Iraq. Two thousand and five hundred and forty-one fecessamples examined using both direct smear and flotation methods using a saturated salt solution for children aged 1 to 15 for the period from December 2017 to November 2018. The results showed that the total infection percentage of intestinal parasites was 18.93%. Five species of intestinal parasites recorder: Entamoebahistolytica (14.60%), Giardia lamblia (3.90%), Trichomonas hominis (0.16%), Hymenolepis nana (0.59%) and Enterobius vermicularis (0.08%). The percentage of infection in male was higher than that of females and the percentage of intestinal parasitic infection fluctuated during the study period. The incidence of infection in the age groups of infected children also affected. The statistical analysis showed significant differences (P< 0.05) between the percentage of intestinal parasitic infection with sex and age of children as well as study months and species of parasites recorded. To conduct the hematological and immunological study, 59 infected children selected (30 males, 29 females) and 31 non-infected children (20 males and 11 females). The blood and serumsof these children obtained and examined by a blood analyzer for the hematological parameters and ELIZA method for immunological parameters. The hematological parameters included RBCs, Hb, PCV, WBCS, Neu., Ba., Es., Ly., Mo., Plt., MCV, MHC, and MCHC. The results of the current study showed the effect of the infection of intestinal parasites on some of the hematological parameters, whether increase or decrease (P < 0.05). The results also showed that other hematological parameters did not affect that infection. The results showed a significant increase (P < 0.05) in IgE, IL-5 and histamineconcentrations in children infected with intestinal parasites compared to non-infected children We conclude from the results of the present study that the infection of intestinal parasites plays an important role in stimulating the immune mechanisms of the body and thus the production of immune materials can harm the body's various tissues.
563 BIOCHEMICAL STUDY FOR PATIENT WITH MAINPROTOZOA DIARRHEAL AGENTS IN BABYLON PROVINCE, IRAQ, Maher Ali Al-Quraishi, Samar Raffia Al Hrbey
The current study during period was conducted from1/September 2018 till 1/February 2019 examination of 60 stool samples (20 samples watery diarrhea for patient with intestinal protozoa,20 samples watery diarrhea for patient without intestinal protozoa and 20 semi sold stool samples for healthy persons, It was examined by direct smear, Ziehl–Neelsen method (acid fast stain ) by using light microscope and Rapid test (chromatography immune assay For make sure that presence injury of those parasites or not,With measured ofSeverity of injury, The samples was taken from (adult 20-40 years male and female),The tests were carried out in the Advanced Parasitology Laboratory - Department of Biology- college of Science / University of Babylon. The current study. The samples was filtrated by tow stapes, At the beginning by filter paper then by sterile syringe filter ,The filtrated liquid examined to determine activity of amylase and lipase enzymes, The result showed thatthere are different between activity of two enzyme in stool samples with intestinal parasite and the other samples (healthy, diarrhea without parasite),And the results showed that there are A positive relationship between the level of activity of the studied enzymes and the severity of intestinal parasites.
564 Association of Anti-Mullerian Hormone and 25-Hydroxyvitamin D in women with low bone mineral density in Babylon, Iraq, Noor Ali Khalil Al Saffar, Hanan AJ Al Taee
Background: Decrease Anti-mullerian hormone and vitamin D was linked to increased risks of osteopenia and osteoporosis. Aim of the study: To find any association of Anti-Mullerian hormone and 25-hydroxyvitamin D serum levelswith bone minerals density test results in women. Material and Methods: The study was design as a cross-sectional; it includes one hundred forty five females, their age range from 23-68 years. These females attended Rheumatology department of Merjan Medical City in Babylon /Iraq through the period from September 2017 till March 2018.Hormonal profile (anti-mullarian hormone, and vitamin D) were assessed; and minerals density of the bone was measured by dualenergy x-ray absorptiometry for all participants. Results:The incidenceof osteoporosis and osteopenia in our studied population was(31, 72%, 23.45%) respectively and (44.83%) were with normal resultof bone mineral density study. Significant positivecorrelation (p
565 Association of Anti-Mullerian Hormone and 25-Hydroxyvitamin D in women with low bone mineral density in Babylon, Iraq, Noor Ali Khalil Al Saffar, Hanan AJ Al Taee
Background: Decrease Anti-mullerian hormone and vitamin D was linked to increased risks of osteopenia and osteoporosis. Aim of the study: To find any association of Anti-Mullerian hormone and 25-hydroxyvitamin D serum levelswith bone minerals density test results in women. Material and Methods: The study was design as a cross-sectional; it includes one hundred forty five females, their age range from 23-68 years. These females attended Rheumatology department of Merjan Medical City in Babylon /Iraq through the period from September 2017 till March 2018.Hormonal profile (anti-mullarian hormone, and vitamin D) were assessed; and minerals density of the bone was measured by dualenergy x-ray absorptiometry for all participants. Results:The incidenceof osteoporosis and osteopenia in our studied population was(31, 72%, 23.45%) respectively and (44.83%) were with normal resultof bone mineral density study. Significant positivecorrelation (p
566 THE USAGE OF ELASTIC TRACTIONS AND CLOSED METHODS IN TREATMENT OF SUBCONDYLAR FRACTURES OF TEMPORO MANDIBULAR JOINT: A COMPARITIVE STUDY, Muntathar Muhsen Hassan Abusanna, Zina Ali Daily, Nawres Bahaa Mohammed
Fractures of the facial bones are very common. The mandible (lower jaw) and zygoma (cheek bone), by nature of their location and anatomy are the two bones most commonly fractured. In the developed world, interpersonal violence is the most common cause of facial fractures; this is often exacerbated by the use of alcohol or illicit drugs.Treatment of condylar fractures of TMJ today seems to be based mostly on three main forms of treatment for patient suffering from condylar process fractures; conservative, closed method, and open method. Aim of the study: The purpose of this study is to make comparison between splint with elastic tractions and closed method in treatment of subcondylar fractures of TMJ. Patients and methods: The study groups are divided into two groups, group A and group B, according to the method of treatment. Group A including 8 patients (all of them were male), (the mean of age 25.5 years), Treatment plane for those patients done by using splints with elastic traction. Group B including15 patients (13 male & 2 female), (mean of age 23 years), Treatment plane for them was done by inter maxillary fixation only (IMF).Those patients were collected during the period from November 2017 to Jun 2019, from the specialized surgery clinic. At the end of follow up period, the clinical parameters including (occlusion mouth opening, deviation, and pain) with radiographic finding of condylar fractures (reduced, reduced with little deviation and still deviated (no results). Results: This study were ,in group A, only one patient (12.5%) out of 8 with slight malocclusion, 2 patients (25 %) showed reduce mouth opening, 1 patient (12.5%) with slight deviation to the fracture side during wide opening, and 1 patient (12.5%) was complain of pain in fracture site.
567 The effect of muscle stretching exercises on some immunological blood indicators of the posterior connective muscle of footballers’ thigh, Abdullah Hawail Farhan Alkabi, Safaa Kazan Radi
The study aimed at the numbers of some positive and negative lengthening exercises and their effect on some immune blood variables of the posterior connective muscle of the thigh for soccer players in addition to exercises for muscle strength and therapeutic methods for working muscles to see their effect on increasing motor flexibility and response of working muscles in all corners of the joint, the study was conducted on the same It consists of ((16 players representing Al Diwaniyah Sports Club in football, they were chosen in an intentional manner, and the experimental method was used by (6) weeks at the rate of (2) training units per week and the time of the main part of the daily training unit ranges at the rate of ( 30-30 minutes) The researcher used statistical treatments in the SPSS statistical program and concluded the following: 1. Continuous stretching exercises reduce muscular tension, ease, speed of movement, reduce pressure on them during intense physical exertion, and increase blood circulation activity. The most important recommendations: 2. The necessity of using this study to strengthen muscles and stimulate the blood circulation of the posterior and anterior connective muscle of football players. 3. The necessity of conducting similar studies for group and individual games.
568 Clinical and therapeutic studies on Bovine Papillomatosis in Al-Najaf Province, Iraq., Abd-Alhadi Jaithom Marzok, Ahmed Jassim Mohammed, Hala B. Thannoon Al-Bayati
Bovine papillomatosis is a contagious viral disease of skin and mucosa, characterized by growth of multiple benign tumour named warts, caused by BPV types 1 to 10. The diagnosis performed by clinical and pathological examinations of the lesion. The prevalence of bovine papillomatosis in A-Najaf was 2.68 %. The most frequent location of the lesions was on the neck, back, right side of the face, around the eyes and the least frequent is the body as a general. Autogenous vaccine therapy was evaluated, in which the lesion was taken from the animal and prepared then injected into the same animal to treat it. Each animal was given three doses, each week the animal injected one ml of the vaccine given intradermaly.About 90% of papilloma lesions were disappeared (cured) one month after the third dose of the vaccine inthe same animal that we took the lesion from it, while it was 70% after a five weeks of last dose of treatment in cases that treated with a vaccine prepared from another animal. We concluded that there is a considerable prevalence of bovine papillomatosis in the region, and the therapeutic effect of the vaccine on the same animal is more rapid and definitive than that of another animal, this allowed recommending of using vaccine of multiple strain of virus in the vaccine preparation.
569 Determination of patient’s knowledge with diabetes type 2 for self- management regarding control blood glucose level, Qasim Ali Khasal, Amer Muhasin Nasir, Dheyaa Kadhim Jabbar
Diabetes Mellitus is a metabolic disorder of multiple etiologic factors characterized by chronic hyperglycemia with disturbance of carbohydrate metabolism. It can play a vital role in the cause of morbidity and mortality through continued clinical consequence. Therefore, good knowledge of glycemic control is necessary for promoting care, enhancing better therapeutic outcomes, and in the prevention and management of diabetes complications. Diabetes self-management is necessary to ensure optimum Blood glucose level Control. However, limited data were available regarding the practice of self-management by Iraqi diabetic patients. A descriptive study was carried out to determine if the Patient’s Knowledge with diabetes type 2 for Self- Management Regarding Blood glucose level Control. From 2nd January, 2018 to 2nd July, 2019 in order to achieve the objectives of the present study. Including (111) males and (89) females. The data were collected by utilization of the study instruments and employment of scheduled interview as means data collection. The data collection process was performed from 2nd January, 2018 to 2nd July, 2019. A questionnaire was designed constructed by the researcher to measure the variable. The questionnaire consisted of 2 parts which are demographical, and diabetics’ self-care skills indicator toward blood glucose level control. The reliability of the questionnaire was determined through a pilot study and the validity through a panel of experts. The data were analyzed through the application of descriptive statistic frequency, percentage, and the application of inferential statistical procedures, which include the Pearson correlation coefficient, and contingency coefficient. This study shows the highest percent of the study samples were (50-59) years old with the mean age (34.5) years, most of them were married male. With a low educational level. With the group's duration are (1-5) years. Insufficient monthly income, unemployed, overweight, they had information from the physician. Also, they were diagnosed incidentally when they measure blood glucose during the physician visit. Most of the study samples were presented with a lack of knowledge regarding self-care management toward blood glucose level control by patients with diabetes type 2 diseases. There is a significant association between self-management toward blood glucose level control and (age, educational level, monthly income). While no significant with another variable. New diabetic patients should be engage in educational program by nurse & supply with booklet which include self- care skills toward blood glucose level control and supported by videotapes to enforce their practices, with the nurse supervision during visit them to the center, instructed to control their blood glucose level, and body weight to improve their self-care skills to avoid complications.
570 EXTRACELLULAR SYNTHESIS OF ZINC OXIDE NANOPARTICLE USING Pseudomonas aeruginosa AND STUDY OF THE ANTIMICROBIAL AND ANTIBIOFILM ACTIVITIES, Thooalnoon Y. Al-Janabi, Esam J. Al-Kalifawi
In this study, ZnONPs were biosynthesized from aqueous zinc sulphate solution through a simple and ecofriendly method using Pseudomonas aeruginosa culture filtrate, which acted as a reductant and stabilizer simultaneously. ZnONPs were characterized using UV-Vis spectroscopy, XRD, FTIR, SEM, AMF, and zeta-potential analysis. The surface plasmon resonance peaks in absorption spectra for ZnONPs colloidal solution showed an absorption peak at 378 nm in a UV-visible spectrum. The functional biomolecules such as carboxyl groups present in the Pseudomonas aeruginosa culture filtrateresponsible for the zinc oxide nanoparticles formation were characterized by FT-IR. The results of AFM show the tip-corrected measured the size of ZnONPs in the range of 30-80 nm. And the 3D view of the sample surface and height distribution around 50 nm. The XRD results suggested that the crystallization of the bio-organic phase occurs on the surface of the ZnONPs or vice versa. The broadening of peaks in the XRD patterns was attributed to particle size effects and the average particle size of ZnONPs can be calculated using the Debye - Scherrer equation, it was found about 20-40 nm. The ZnONPs were scanned using SEM. It reveals that a ZnONPs seems to be irregular and polymorphic where the crystalline forms of these particles varied between rectangular, spherical, radial and cylindrical structures and the average size ranges between 28-43 nm. Measuring the stability of the colloidal solution of ZnONPs gave a value of -51.8 Mv. The result shows the ability of ZnONPs to inhibit the growth and the biofilms of Pseudomonas aeruginosa isolates.We concluded the biosynthesized ZnO NPs which have antibacterial and antibiofilm activities and it can be used as an antibacterial therapeutic agent.
571 INHIBITION EFFECT OF GREEN APPLE PEEL EXTRACTS ON SOME TYPES OF PATHOGENIC BACTERIA ISOLATED FROM VAGINA, Bilal Salim Dawood Al-Taie, Mohammed Yahya Allawi
Escherichia coli and staphylococcus aureus pathogens were collected from 65 vaginal samples of patients aged (15-55). Estimating anti-bacterial effect of Ceftriaxone (CT) and ethanol extract green apple peels (EEGAP) versus E. coli and Staph. aureus through variety of concentrations (125, 250, 500, 1000, 1500, 2000, 2500, 3000) µg/ml each one alone. Furthermore, results obtained by the combination test of CT with EEGAP against E. coli and Staph. aureus, showed that when using concentrations of (250, 500, 1000, 2000,3000) µg/ ml treated with disc dilutions of EEGAP and CT each one alone against E. coli and Staph. aureus respectively, only CT showed inhibitory impact versus two pathogens. The minimum inhibitonconcentration (MIC) of CT at concentration (125) was (1.2 mm for Staph. aureus, R for E.coli), while maximum inhibitory concentration(MXIC) of CT at (3000) was (5.0 mm for Staph. aureus, 4.8 mm for E.coli). In contrast, Staph. aureus and E.coli are R when treated with EEGAP separately. From another hand, the MXIC result seen from the combination of (CT 50% + EEGAP 50% against E.coli ) at 3000 µg/ ml was 16.31%, in addition, (CT 50% + EEGAP 50% against Staph. aureus) at 3000 µg/ ml was 16.33%.
572 ISOLATION AND IDENTIFICATION OF BACTERIAL SPECIES ASSOCIATED WITH ODONTOGENIC INFECTIONS, Mahdi Yakoob Kzar1 , Fatima Malik Abood , Rusul Saleem Abd, Ahmed Mohammed Abas
Odontogenic infection is an infection that originates within a tooth and the closely surrounding tissues as the dental pulp, jawbones, periodontium, or in the closely surrounding tissue. This study aimed to isolate and detect bacteria associated with odontogenic infections and identify antimicrobial susceptibility against predominant bacterial isolates. A total of 30 pus samples were collected by aspiration and placed in transport media. "These pus samples subjected to different methods for identification of bacteria using traditional bacteriological method". The antimicrobial liability of isolated bacteria was determined by using disc diffusion method. Predominant isolated bacteria was Streptococcusviridians group (36%) followed by S. aureus (26.2%) and lowest percentage of isolated bacteria was black pigmented bacteria(Prevotella and Porphyromonasspp), in addition to K. Pneumonia and E. coli. The results showed very high susceptibility of isolates to amoxicillin (97%) followed by cefotaxime and imipenem (94%). Whereas the lowest sensitivity was detected to clindamycin (18%). The present results approve the existence of mixed bacterial infection with the predominance of Streptococcusviridans. Most Streptococcusviridans were inhibited by amoxicillin followed by cefotaxime and imipenem.
573 FUNCTIONAL IMPACT OF ESTROGEN RECEPTOR ALPHA GENE POLYMORPHISM ON POSTMENOPAUSAL OSTEOPOROSIS, Luma M. Edan, Mohemmed A. M. Albayati
Background: Osteoporosis (OP) is one of the most common skeletal disorders which characterized by low bone mass density (BMD) resulting in an increase in the susceptibility to fracture. The etiology of OP is complex and implies the interaction between genetic, hormonal, and environmental factors. Aims: This study aimed to investigate the impact of estrogen receptor alpha (ERα) gene polymorphisms (PvuII and XbaI) on OP development in postmenopausal Iraqi women Subjects and Methods: This is a case-control study which included a total of 84 postmenopausal. The women were divided into two groups: those with OP (42 women) and those without OP (42 women). Peripheral blood samples were collected from all women. DNA was extracted from whole blood using ready commercial kit. Two sets of primers were designed to determine frequency of each polymorphism by quantitative polymerase chain reaction (qPCR) combined with melting curve analysis. The association of different genotypes of these polymorphism with the incidence of OP was analysis using logistic regression test. Results: For the PvuII, the frequency of CC, CT and TT genotypes in controls were 64.29%, 28.57% and 7.14% respectively compared to 45.24%, 50% and 4.76% respectively amongst patients with significant difference in the distribution of heterozygous genotype (OR=2.75, 95%CI=1.09-6.92, p= 0.032). In contrast, there were no significant differences between patients and control regarding XbaI polymorphism, neither at genotypes no at allelic level. The haplotype block TA was more frequent among patients than controls (19.04% versus 7.14%) with a significant difference (OR= 2.9, 95%CI=1.07-7.84, p=0.03). Conclusions: The CT genotype of PvuII polymorphism and the haplotype TA of PvuII and XbaI polymorphisms can increase the risk of OP in postmenopausal women.
574 COMPARATIVE STUDY BETWEEN MULTIPLE DAILY INJECTIONS AND CONVENTIONAL INSULIN REGIMENS IN IRAQI CHILDREN AND ADOLESCENTS WITH TYPE 1 DIABETES MELLITUS, Rihab F. Alabedi
Background: Type 1 diabetes mellitus is the most common endocrine-metabolic disorder of childhood and adolescence. The discoveryof insulin in 1922 make a major breakthrough in therapy of diabetes. There are two known insulin regimens, conventional and the basal bolus regimens. While conventional insulin regimen still used in some areas, it is not the preferred regimen. Management is best accomplished by a multidisciplinary team consisting of child, family, physicians, nurse educators, dietitians, and mental health professionals.Objectives: To assess the effect iof basal bolus regimen on the glycemic control, risk of hypoglycemia and ketoacidosis in comparison with conventional therapy in Iraqi children and adolescents with type 1 diabetes mellitus.Patients and method: A comparative study had been conducted at the pediatrics clinic of the National Diabetic Center , Al-Mustansiriya University during the period from February 2017 to November 2017. Seventy-two patients with type 1 diabetes had been included, of which 40 on conventional regimen and 32 on basal bolus regimen (multiple daily injections). Patients were assessed by HbA1c, hypoglycemic and ketoacidosis episodes.Results: The basal bolus regimen group had more diabetic ketoacidosis (P= 0.035) in comparison to conventional group but didn’t differ in occurrence of hypoglycemic episodes (P= 0.673) and HbA1c (P=0.2).Conclusions: In Iraqi children and adolescents with type 1 diabetes, in comparison with conventional regimen, there was no more hypoglycemic episodes in basal bolus regimen with nearly similar glycemic control as indicated by HbA1c, but it was associated with more diabetic ketoacidosis in the absence of full diabetes team and tools that improve patients' compliance.
575 NUTRITIONAL STATUS OF CHILDREN UNDER FIVE YEARS IN BAGHDAD, Mudher Hasan Noor, Ali HadiMahdi, Khelowd Salih, Jawad Kadhim Hamza, Rusul Faihan Mousa, Sura Falah Abdul Hadi
Nutritional status: is the condition of health of the individual as influenced by utilization of nutrients. Nutrition is a science that studies all the interaction that occur between living organism and food, thepsychological,social,cultural,economic andtechnological factors that influence which foods we choose to eat and the biological processes by which weconsumefoodandutilizedthe nutrient it contains. Nutrients are substance contained in food that are necessary to maintain life and allow growth and reproduction. Nutrients provide energy, contribute to structure, and regulate biological processes. Anthropometric measurements; refers to comparative measurement of human body. This measurement commonly used as indices growth and development for infants include body weight, height/ length, occipitofrontal circumference and midarm circumference. Typically, growth is evaluated by comparing individual measurements to reference standards, represented by percentile curves and a growth chart. Aim of the study Assessment of nutritional status of children under five years in two areas in Baghdad and Compare of nutritional status of children between high socioeconomic area and low socioeconomic area in Baghdad. A cross-sectional study conducts on 200 children under five years (6m to 60m) living in Baghdad to assess the nutritional status of children living in low and high socioeconomic areas. Data collection included interviews the mothers of children focused on demographics and socioeconomic status of the family and anthropometric measurements of the children by using growth chart (Bwt/age, Bwt/height, and height/age), to assess the nutritional status of children. The study shows that the children affected with acute malnutrition (wasting) are more in low socioeconomic area than in high socioeconomic area but there is no significant difference, while children affected with chronic malnutrition (stunting) are more in low socioeconomic area with significant difference. With using arm circumference, we found children with malnutrition are more in low socioeconomic area than children in high socioeconomic area with significant difference. According to the age group, there is significant difference between children under I year, while there is no significant difference between children above 1 year. Malnutrition still a big problem facing Iraqi community in pediatrician, there is a high number of children with acute and chronic malnutrition especially in low socioeconomic area. Children with the chronic malnutrition rather than acute malnutrition are morein low socioeconomic area than in high socioeconomic area with significant difference.
576 Evaluation of Urinary Monocyte Chemoattractant Protein-1and Serum Cystatin C as Markers for Detection of Diabetic Nephropathy in Comparison to Albumin to Creatinine Ratio (ACR), Duaa Hataem, Raad Abudlameer Alasady, Safaa Ali Khudhair
Background: Diabetic nephropathy is known as diabetic kidney disease, is one of the most common microvascular complications of the kidneys resulting from type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM).Aim: Evaluate the diagnostic value of Monocyte chemoattractant protein-1and cystatin c as markers for detection of diabetic nephropathy. Materials and methods: A total of 119 participants diagnosed with T2DM were recruited and these participants were classified into three groups in relation to their albumin creatinine ratio (ACR) including patients with normoalbuminuria, patients with microalbuminuria and patients with macroalbuminuria. Results :According to the results of this study, there was non-significant difference among diabetic nephropathy groups and Cystatin C or MCP-1.Receiver operating characteristic (ROC) curves showed that for primary detection of diabetic nephropathy, the best cut-off value to differentiate diabetic with nephropathy and diabetic without nephropathy groups were ≤ 207.28 pg/ml of Cystatin C with 82.1% sensitivity, 50% specificity, and area under the curve (AUC) =0.659; ≤ 3.15 pg/ml of MCP-1 with 67.3% sensitivity, 50% specificity, and area under the curve (AUC) =0.546, while > 101 pg/ml of eGFR with 42.5% sensitivity, 83.3% specificity, and area under the curve (AUC) =0.528.Conclusion:We conclude that there was no association between serum Cystatin C or urinary MCP-1 and albuminuria.
577 The Antimicrobial Effect Of Ethanol And Methanol Silver Nanoparticle (AgNPs) Colloidal On Enterococcus Faecalis Isolated From Endodontic Infections In Najaf Provence. An In Vitro Study , Dheyaa ShnanAl-Jameel, Bassim Mohammed Ali Alaasam , Wasna’a M. Abdulridha
Enterococcus faecalisis one of the mostcommon causative agent of endodontic infections and this study aimed to investigate the antibacterial activity of silver nanoparticles (AgNPs) which prepared by ethanol, methanol, and ethanol-methanol v/v as a dispersing solutions. The 3-D AFM image of AgNPs had 55nm average particle size. The roughness average for AgNPs layer was 0.462 nm while the root mean square roughness was 0.57nm. Out of 80 patients with endodontic infections their ages ranging from 7-80 years only five (6.25%) E. faecalis isolated and the antibacterial activity result showed by disc diffusion methods, the best activity is for ethanol-methanol AgNPs colloidal with diameter of inhibition zone 20.8 ± 1.643 mm (Mean ± SD) which is significant compared with control treatment 13.6 ± 1.140 mm, while for ethanol AgNPs colloidal and methanol AgNPs colloidal are 15.4 ± 1.140mm and 13.2 ± 0.836mm compared with control treatment 10.6 ± 0.547mm and 9.0 ± 0.707mm respectively. The MIC of ethanol-methanol AgNPs colloidal is 20μg/ml and ethanol AgNPs colloidal and methanol AgNPs colloidal are 30, 60 μg/ml and for control treatment are 50, 60, and 80 respectively, all the result are significant in the level of p≤0.05. the result of antibiotic susceptibility showed that 80% of isolate are susceptible for Chloramphenicol, Carbenicillin and Ampiclox while its completely resistant for Metronidazole and Bacitracin.
578 Conservative versus Surgical Treatment of Perforated Peptic Ulcer, Ayoub Motlak Zedan, Maan Harbi Head, Basim Ghaib Hussein
Background: The perforated peptic ulcer could be treated surgically or conservatively. The treatment of choice is surgical treatment by simple omental patch. Nonsurgical treatment of a patient with a perforated peptic ulcer has proved good outcomes in selected patients with the well general condition. Objectives: To determinewhether surgical treatment can be avoided in some patients of perforated peptic ulcer with selected criteria. Patients and Methods: A prospective study included thirty-nine patients scheduled for treatment of perforated peptic ulcer, attended Salah Addin General Hospital, during the period from June 2017 to December 2018. Fifteen patients treated surgically and 24 patients treated by nonsurgical treatment consisting of nil by mouth, nasogastric aspiration, intravenous fluid, intravenous antibiotic, and intravenous anti-ulcer drugs. The comparison was made between surgical and nonsurgical groups. Results: The total number of patient in our study was 39 patients. Most joint age group was 40-70 years. Fifteen out of 39 patients underwent immediate laparotomy, 24 out of 39 patients Included in conservative treatment. The results of conservative treatment were 18 patients (75%) in the successful group and 6patients (25%) in the failure group. There is no significant difference between surgical and nonsurgical groups regarding complications and median hospital stay.There is no mortality in the nonsurgical group and only one death in the surgical group. Conclusion: The surgical treatment of perforated peptic ulcer could be avoided in some patients. The perforated duodenal ulcer is more common than a perforated gastric ulcer. The perforated peptic ulcer is more common in male than in the female.
579 Study the prevalence of mecA gene in methicillin resistance staphylococcus aureus (MRSA) isolated from different clinical specimens and their antibiotic resistance profile, Basima Q. Hasan Al Saadi , Seham H. Mohaisen, ZainabT. Kazaal, Zeena N. Abdulla
Methicillin-resistant S aureus has progressed into an important pathogen of humans and is endemic in hospitals worldwide. MRSA strains carry multiple antibiotic-resistant genes. Recent work aim to investigate the prevalence of mecA gene among clinical isolates of MRSA .Fifty isolates of Staphylococci were demonstrated as methicillin resistancestaphylococcus aureus from various clinical specimens ( urine ,blood ,wounds ,and burns )after confirming their identity using morphological and biochemical tests ,as well as diagnosis by VITEK2 system. Also antibiotic sensitivity testing performed for all isolates by disc diffusion methods.Fifty isolates of S. aureus classified as MRSA taken from different clinical cases.mecA gene was detected by PCR technique to amplify 533bp using specific primers for the gene. The results showed that MecA gene was detecting in 39(78%) of Staphylococcus aureus isolates.Most of MRSA isolates were multiresistant to three antibiotic classes beta-lactams and multidrug resistant to other common antibiotics, such as aminoglycosides, therefore this work emphasis that there are other possible resistance mechanism may interact with mec A gene and causes development of methicillin resistance staphylococcus aureus
580 Kasim Traim1, Firas Abdullah Noori 2 and Alaa Jamel Hasin*3, Kasim Traim, Firas Abdullah Noori, and Alaa Jamel Hasin
Background: High Fistula-in-ano is a common surgical problem, it is regarded a challenge to the surgeonsdue tocarry a high risk of recurrence and complications especially of anal incontinence, theaim of the surgical procedure is to altogether remove the fistula, prevent recurrence and avoiding the main complications like anal incontinence. The need to reduce the anal sphincter damage lead to the use of fibrin glue, collagen plug, clip closure, mucosal flap advancement techniques, ligation of intersphincteric fistula tract (LIFT), Video-assisted anal fistula treatment (VAAFT) and Fistula laser closure (FiLaCTM). Carbon dioxide (CO2) laser used as a tool in the treatment of anal fistula. The CO2 10600nm laser allowing increased surgical precision and accuracy, thin depth (0.03mm) penetration, thereby reducing unnecessary damage to underlying tissues, and the procedure was with no bleeding, resulting in improving visualization of the surgical field, eliminating the need for electro-cautery which can cause more damage, also closed the lymphatic and nerve endings reduce the oedema and pain. The aim of this study is to evaluate the benefit of CO2 laser to remove anal fistula. Methods: 26patients between 23 to 45year old with a mean age of 32-year-old (SD±6.4) with high fistula in ano measured 7.5 to 10 cm, mean 8.41(SD±0.85), all were direct fistula except one was complicated & only one was a recurrent fistula. Operation (fistuloctomy) was done under spinal anaesthesia, Result: On following all patients, the pain was moderate in fourpatients, no primary bleeding, no recurrence or faecal incontinence; all patients started their Routine work in about fivedays. Conclusion: The CO2 laser may a good tool for anal fistula excision because of almost bloodless surgery, reduce the risk of infection and precisely controlled surgery which limits injury to surrounding tissue.
581 EVALUATE THE TWO WAVELENGTH OF Nd: YAG (532-1064NM) LASER TO REMOVE THE TATTOO FROM HUMAN SKIN, Hana K Hasan
Nd:YAG laser wavelength (532-1064nm) was used tattoo removal. The result of treatment tattoo removal to different type. This laser is better than by using ER: YAG or IPL. By using this process the skin later are absorbed laser beam, especially in superficial treatment these laser532nm. Therefore, there are many different characteristic, which related to the skin layer, such as the thermal and thermal relaxation time (TRT) and the target (TRT) time, which is required for the heated tissue that used to lose about half of its heat.
582 EFFECTS OF THE SYSTEMIC ADMINISTRATION OF OMEGA -3 POLYUNSATURATED FATTY ACID ON EXPERIMENTAL PERIODONTITIS: A histopathological study for rat’s periodontal tissue, Aveen Ageel Jalal, Zewar A.AL-Qassab, Rafel A.Al-Rawi
Periodontitis is a common inflammatory disease characterized by the formation of periodontal pocket and can lead to bone and tooth loss.The aim of this study was to evaluate the effect of omega-3 fatty acids (Eicosapentaenoic acid) as a treatment for ligation induced periodontitis using an animal model. Periodontitis was induced for the studied animals by ligation around the upper central incisor. Twenty five animals were used for the pilot study, and the eighty studied animalswith the induced periodontitis were divided into four equal groups according to the treatment used: Water, scaling/root planing, 60mg/kg eicosapentaenoic acid, and scaling/root planing with eicosapentaenoic acid. After three days, two, four, and six weeks treatment, five animals from each group were sacrificed, and the maxillae were collected, processed, and analyzed. The treatment by scaling and root planingtogether with the eicosapentaenoic acid can significantly reduce the periodontal damage index after four weeks, but whenthese treatment was used each one alone, it causes significant reduction in damage score after six weeks. For short duration of periodontal treatment, eicosapentaenoic acid can be used with scaling and root planing as a good treatment for periodontal disease.
583 THE IMPACT OFTHE HLA-G 14 BP INSERTION /DELETION POLYMORPHISM AND SOLUBLE HLA-G SERUM LEVEL IN SYSTEMIC LUPUS ERYTHEMATOSUS, Ikram Khazal Qasim Al- hasso, Aida Rashid Al- Derzi, Ahmed Abdul-hassan Abbas, Faiq Isho, Ahmed Sameer Alnuimi
Human leukocyte antigen-G (HLA-G) expression has been suggested as a possible mechanism of tissue protection against autoimmune diseases by the interactions between soluble HLA-G)sHLA-G( proteins and their specific inhibitory receptors. The aim is to investigate the role of the HLA-G 14bp insertion/deletion polymorphism and the sHLA-G serum level in the immune-pathogenesis of SLE. A total of35 SLE patients and 35apparently healthy individuals as controls were enrolled in this study. The HLA-G 14bp insertion/deletion polymorphism was determined by the polymerase chain reaction (PCR), while the serum levels of the soluble HLA-G were detected by the ELISA technique. The SLE patients in this study were among the patients who attend the Rheumatology Clinic, Rheumatology Ward in Baghdad Teaching Hospital and Private Nursing Home Hospital in Baghdad Medical City. This study was carried outthrough the period from December 2018 to November 2019. There was no statistically significant difference in the allele frequency of HLA-G14-bp insertion (p=0.11) and deletion (p=0.58) alleles frequency between SLE patients and controls. There is a trend toward a slight increase in the 14bp insertion allele frequency and insertion/insertion genotype in patients as compared to controls. The soluble HLA-G level in SLE was significantly lower than that in healthy controls (p
584 RISK FACTORS AND OUTCOMES OF NEONATAL JAUNDICE AT AL-RAMADI TEACHING HOSPITAL FOR MATERNITY AND CHILDHOOD, Rana F. Shitran,Maysam Y. Abed
Jaundice’ is a yellow-orange discoloration of the skin and sclera because of excessive bilirubin in the skin and mucous membrane. Jaundice caused by indirect neonatal hyperbilirubinemia (INH) is a common and a frequent cause of neonatal admission to health care facilities all around the world. The main aim of this study is to determine the prevalence of neonatal jaundice and explore its risk factors in term and preterm neonates In this cross-sectional study Newborns sampled after delivery and before discharge that developed hyperbilirubinemia and required phototherapy and/or exchange transfusion within the first 14 days of life included in this study. Those who do not require phototherapy excluded from the study. The investigation done according to clinical feature, and include Complete blood count, serum bilirubin, blood group, TSH, Coombs test, reticulocyte count, ABO, Rh, G6PD and TORCH screening The total number of study patients was 487. All of them were neonates diagnosed with neonatal jaundice. In table (1), 47% of patients were aged between 3 – 5 days; 58.7% of them developed jaundice at age between 1 – 3 days; 60.4% were males; 43.5% were delivered at Summer season; 76.8% of them were full-term; 83.6% were weighed ≥ 2500 gm; 55.2% were delivered by NVD; 37.6% were fed by breast and bottle feeding; 2.1% showed kernicterus signs; 9.4% needed
585 GENOTYPING OF GLUTATHIONE-S-TRANSFERASE T1 AND M1 IN IRAQI BEHÇET'S DISEASE, Hawraa Sabah Al-Musawi, Fulla AbdAlsattar Alriyahee, Zahraa Saleh Kamil, Mona Al-Terehi
The Behçet's disease (BD) one of the multisystem diseaseswhich usually presents with oral aphthous and genital ulcers,the elevation offree radicals productionanddecreased efficiency of antioxidant defenses have major role in this disease.The present study was carrying out to assess the possible association between BD disease and glutathione S-transferase Mu1 (GSTM1), glutathione S-transferase T1 (GSTT1) gene polymorphisms using multiplex PCR. The results of present study show four polymorphismofgenes, GSTM normal,GSTT normal, GSTT with GSTMnormal and gene deletion,GSTM gene was normal in 48% and 10% for patients and control. While 90% of patients were deleted in significant differences (p
586 SERUM LEVEL OF HMGB1 VERSUS DEMOGRAPHIC AND FEBRILE CONVULSION CHARACTERISTICS IN IRAQI CHILDREN, Manal M. Kadhim, Ali H.Khlebos, Noor Maki Jabar Alsulaiman
Febrile seizure (FS) is the most common type of seizure in childhood that occurs in 2-5 % of the children younger than 6 years. Fever is induced by pro-inflammatory cytokines during infection, and pro-inflammatory cytokines may trigger the development of febrileseizures.HMGB1 contributes to febrile inflammatory responses. There are conflicting results on increasing HMGB1 in serum during FS. One group 72 febrile children (6 months to 5 years old) and other group consisting of 80 children without seizure which served ashealthy control group. Blood samples were collected from the febrileseizure child patients within 30 minutes of the time of the seizure members of both groups and serum samples were prepared; HMGB1 concentrations were measured using Enzyme-linked immunosorbent assay (ELISA) kit. Serum HMGB1levels were significantly higher in febrile seizure patients than in healthycontrols. Serum HMGB1levels were no significant for dimorphic characteristics (Temperature, type of FS, duration of FS, recurrent of FS, family history of FS or epilepsy).
587 MOLECULAR DETECTION OF BLACTX-M GENE AMONG PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM DIFFERENT CLINICAL SAMPLES IN ERBIL CITY, Fattma A.Ali, Bashdar M. Hussen, Sahar Muhammad Zaki
Increasing prevalence of infections caused by multidrug resistant Pseudomonas aeruginosa due to production of Extend spectrum and Metallo-β-lactamases is a major concern throughout the World,is a serious problem and calls for an effective infection control measure to curb their dissemination,the prevalence among clinical isolates varies greatly worldwide and they are rapidly changing over time. The aim of the present study is to investigate the presence of the blaCTX-Min clinically isolates of P.aeruginosa strains producing Extend spectrum and Metallo-β-lactamases. A total of 227 samples were collected from different clinical specimens (sputum, urine, wound, burn, Bronchial wash) attending public hospitals in Erbil provincebetween (September 2017 – March 2018).isolated microorganisms were processed and identified using standard bacteriological conventional culture methods, biochemical tests, Species identification and antibiograms for pathogens were performed using Vitek 2 system against 15clinically important antibiotics. ESBL producing Pseudomonas aeruginosa isolates were detected using Vitek 2 system and also the screened isolates were further phenotypically studied for MBL production was detected by performing combined disc test by using imipenem discs with and without ethylenediaminetetraacetic acid (EDTA), which chelates zinc required for Bacterial genomic DNA was extracted, and the presence of the blaCTX-M were detected by PCR. Out of 227 samples 40 (17.6%)isolates were positive for P. aeruginosa,the highest isolation rate (2.6%) was observed in burn specimens, high rate (> 90%) of antibiotic resistant P.aeruginosa isolates were observed for penicillin, amikacin, piperacillin, lincomycin, vancomycin, chloramphenicol, rifampicin ,ciprofloxacin, Erythromycin. The prevalence rate of ESBL in our study 32 (80.6%) isolates, 72.5% MBL production by P. aeruginosa was observed and co- resistance was recorded in 21(52.5%). Our results demonstrated that cefotaximase blaCTX-M ESBL protein was expressed in 28 (70%) P. aeruginosa isolates with size(499bp), In addition, high prevalent of blaCTX-M gene was observed in burn patient (25%). The present study underlines the unique problem thatthe emergence of ESBL and MBLs and their broad spectrums and unrivalled drug resistance is creating a therapeutic challenge for clinicians and microbiologists. Based on the results of this study, it is suggested to find ways to prevent blaCTX-M gene activity in order to prevent resistance Hence, Due to the advantages of molecular methods in the diagnosis of opportunistic pathogenic bacteria; it could be convenient and swift technique to prevent the progress of infections and mortality among these patients.
588 ASSESSMENT OF POST COITAL TEST IN FEMALES WITH UNEXPLAINED INFERTILITY IN BAGHDAD CITY 2018- 2019, Aseel Khalid Hameed, Ban Nadhum AbdulFatah
About 200 to 300 million sperms transverse from the vagina to the cervix, only few hundreds of them can achieve the oocyte. The aim is the assessment ofpost coital test in females with unexplained infertility. In this study, 260 infertile women during 14-16- day of the cycle were considered. The women were subjected to the post-coital test at 2 hours after intercourse. The study was conducted in Baghdad during the period from January – April, 2018-2019. The study revealed that less than half of females were over 35 years and more than half were educated from urban areas a