|1 DIABETIC WOUND HEALING MANAGEMENT- A PEER REVIEW, Harshavardhan Pathapati, T.E. Gopala Krishna Murthy, B. Ramanaiah, Davu Srinivas
Objectives: Diabetes is a metabolic disorder mainly impairs the body glucose utilization capacity due to this perforcely repressing the immuno-dysfunction (decreases chemotaxis, phagocytosis and intracellular killing actions) and collagen synthesis which are essential in wound debridement management of diabetic patients. Delayed wound healing is considered as one of the most repulsive disabling and costly complication of diabetes. People with diabetes have extenuated circulation, poor resistance to infection and mitigate local nutrition, thus their wounds are meticulously susceptible to infection. Moreover diabetes agonizes the equilibrium exists between accumulation of extra-cellular matrix components and their re-modeling by matrix metallo-proteinases (meltrin), due to this extenuated proliferation action of fibroblasts and finally freezes the progress of wound healing frequency in hyper glycemics. However in diabetic persons the nervous system becomes numb and all feasible actions of neurons are skipped, that condition is called as diabetic neuropathy. In that situation patient body features elevated a glucose level which stiffens the arteries and lack of pain sensation in foot resulting in commencement of new wounds. Conclusion: Consider all problems associated with wound healing in diabetic persons, a proper wound healing management which includes controlling measures like optimized systemic and local factors as well as implement suitable wound dressing for necessary wound in diabeties patients.
|2 STUDIES ON THE PHYTOCHEMISTRY, SPECTROSCOPIC CHARACTERIZATION AND SCREENING FOR ANTI-MITOTIC EFFICACY OF SALICORNIA BRACHIATA ROXB, G.V.Pavan Kumar,B.Narendra Naik, R.Ninitha Sri,B.Pooja, B.Brahmaiah, D.Srinivasa Rao
Salicorniabrachiata is a euhalopytic plant belonging to the family Chenopodiaceae. The present study investigates the phytochemistry, characterization and antimitotic activity of ethanolic extract of S.brachiata.Plants popularly known as Sea asparagus are cooked and eaten or pickled. It is also a good fodder for cattle, sheep and goat. Plant material is also used as raw material in paper and board factories. Its seeds yield high quality edible oil which is highly polyunsaturated and similar to safflower oil in fatty acid.S.brachiata was collected from the back waters of Bapatla,Guntur district. The collected plant material was shade dried and pulverized. The plant material Was studied for phytochemistry,spectroscopic analysis i.e.,UV- Visible, FT-IR and anti mitotic activity.S. brachiata has been prescribed in traditional medicines for the treatment of intestinal ailments, nephropathy, and hepatitis in Oriental countries. In addition, S.brachiata has recently reported to be effective on the atherosclerosis, hyperlipidemia, and diabetes. A variety of pharmacological experiments have revealed that solvent-extracted fractions of S.brachiata exhibited anti-oxidative, anti-microbial, anti-proliferative, and anti-inflammatory activities,supporting rationale behind its several traditional uses.The phytochemical analysis indicates the presence of Tannins and Flavonoids in the plant. UV-Vis Spectrum, used for the quantitative analysis of the plant extract showed peaks at 280 and 290 nm. Identification of the functional groups was performed by FT-IR spectroscopy which confirmed the presence of phenolic, alcoholic and aromatic compounds.
|3 SIMPLE UV SPECTROPHOTOMETRIC ASSAY OF CIPROFLOXACIN, Safila Naveed and Nimra Waheed
Objective:Ciprofloxacin is an established broad-spectrum fluoroquinolone antibiotic displaying activity against both Gram-positive and Gram-negative bacteria pathogens. It acts principally by inhibiting DNA-gyrase and topoisomerase IV present in bacterial cells which are responsible for reproduction of DNA of bacteria. A rapid, simple, accurate, and economical least time consuming spectrophotometric method has been developed for the assay of Ciprofloxacin and then compare it with the assay of different brands available in Karachi, Pakistan. A sample of drug was dissolved in water to produce a stock solution containing ciprofloxacin.Methods: Samples of ground tablets of different brand were taken and their diluted solutions of 100ppm, 50ppm , 25ppm,12.5ppm and 6.25ppm were prepared with the same solvent. The assay was determined by measuring the absorbance of stock solution against the solvent blank and comparing with the absorbance of available brands of ciprofloxacin at the wavelength of 278 nm by spectophotometer. Results: The percent assay is calculated and the correlation coefficient for different brands was found to be 0.9998 for (A), 0.9992 for (B), 0.9996 for (C) and CYCIN (D), 0.9989 for (E) and 0.9986 for (F) which are within the limit. Conclusion:Thus we can conclude that the method can be applied for the routine QC quantitative analysis of ciprofloxacin in active and tablet formulation.