• We are available for your help 24/7
  • Email: info@isindexing.com, submission@isindexing.com

Paper Details

Activation and Inhibition of Staphylococcus aureus Group 2 AgrC by synthetic AIP and its derivatives

Ayman M. AL-Ghamdi

Journal Title:Basic Research Journal of Microbiology

Staphylococcus aureus is one of the main significant pathogens that are wide spread in human environment. It is responsible for several diseases some of which are considered as life threatening. Although many antibiotics are available, there is the possibility that the organism may develop a new mechanism to evade the effect of those drugs. Many bacteria have been recognised as multiple antibiotic resistant which increase the need to invent a new techniques that can be used to control the spread of those infectious organism. In fact, using quorum sensing (QS) to dominate virulence factors has been shown to have good results. In this project, a group 2 agr bio-reporter was constructed after identifying the correct start codon of the AgrC gene. Then several synthetic AIPs were used in order to activate or inhibit the bio-reporter. There was an obvious inhibition by using AIP-I and Ala5 (AIP-I), while using AIPI-IV presented no inhibition against AgrC-2 activation. A new AIP molecule (CpgAIP73) was used in this study which showed very little inhibition by using the highest concentration (100nM). It is becoming increasingly important to create potent antibacterial agents; inhibition of QS is an excellent mechanism to potentially reduce the virulence of these bacteria Keywords: Pathogens bacteria- Staphylococcus aureus - synthetic AIP AgrC-2 inhibition