Phenotypic and molecular characterization of white calla lily soft rot caused by Pectobacterium carotovorum subsp. carotovorum
Nazerian E, Biramizadeh E, Zarei R, Hosseini Nia A, Shafiei MR
Journal Title:Basic Research Journal of Microbiology
A destructive disease on white calla lily was observed in commercial ornamental plants greenhouses in Markazi province, Iran. Disease symptoms on leaves and stems were gray to pale brown discoloration and expanding water soaked lesions on stem. PCR amplification of pectatelyase encoding gene produced a 434bp banding pattern on 1% agarose gel. The causal agent identification was confirmed by sequencing of the Pel gene. The ITS-PCR products were digested with RsaI restriction enzyme. For discrimination of the P. carotovorum from Pectobacterium odoriferum, all isolates were subjected to ?- methyl glucoside test. All isolates were identified as Pectobacterium carotovorum subsp. carotovorum. Keywords: Molecular detection, Identification, White calla lily, Soft rot.