Pullulan production from Aureobsidium pullulans by continuous culture

Hani Moubashera and Salwa S. Wahsh

Journal Title:Basic Research Journal of Microbiology
Abstract

Ten isolates of Aureobasidium pullulans were screened for the highest production of pullulan and minimal melanin production from Fayoum Governorate, Egypt. The optimum conditions for continuous culture were studied; temperature was 24°C, while pH 5.0, and agitation speed was at 500 rpm, while the aeration rate was 2.5 vvm and the best flow rate was 1 L/ 24 h. The maximum production of pullulan concentration with minimum melanin production was reached 10.18±0.04 gl-1. Keywords: Aureobasidium, pullulan, Continuous culture, Aeration, Flow rate, pH.

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