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Paper Details

Antioxidant, an anti-inflammatory and anti-arthritic activity of Centella asiatica extracts

Seema Chaitanya Chippada1and Meena Vangalapati 1*

Journal Title:Journal of Chemical, Biological and physical sciences
Abstract


Centella asiatica is a valuable medicinal herbaceous aromatic creeper which has been valued for centuries in ayurvedic medicine. Phytochemical analysis of Centella asiatica plant extracts revealed the presence of various biochemical compounds such as alkaloids, flavonoids, glycosides, phenolic compounds, triterpenoids and saponins etc. Since phenolic compounds, triterpenoids and flavonoids have remarkable anti-inflammatory, anti-arthritic and antioxidant activites, so our present work aims at evaluating the in vitro anti inflammatory activity by HRBC membrane stabilization and in vitro antioxidant activity of Centella asiatica by three in vitro models such as reducing power, nitric oxide scavenging, DPPH assays. The inhibition of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti inflammatory activity. Protein denaturation, membrane stabilisation and protein inhibitory action were taken as a measure of the in vitro anti-arthritic activity. The total phenolic content was found out to be 33.56 1.56 mg/gm GAE. The DPPH free radical scavenging activity, reducing power and Nitric oxide scavenging activities was concentration dependant with IC50 value being 96.15 1.23 ?g/ml, 261.43 0.92 ?g/ml and 106.32 2.19 ?g/ml respectively. The maximum membrane stabilization of C. asiatica extracts was found to be 94.97 % at a dose of 2000 ?g/ml. The maximum percentage inhibiton of protein denaturation, membrane stabilisation and proteinaseinhibitory action for C. asiatica extracts ware found to be 89.76 %, 94.97 % and 91.63% respectively at a dose of 2000 ?g/ml. The results show that the extracts of Centella asiatica exhibited Antioxidant activity, Anti-arthritic activity and anti-inflammatory activities.

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