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Paper Details

Response surface optimization of process variables for alkaline protease production by bacillus subtilis

Tatineni Parimala1, Sneha.P.M1, C.S.V.Ramachandra Rao1, R.Satish Babu1*

Journal Title:Journal of Chemical, Biological and physical sciences

culture medium for the production of bacterial alkaline protease from Bacillus subtilis was developed by Response Surface Methodology (R.S.M). Central composite design was used to optimize the process variables which are responsible for the production of protease. The variables studied for production of protease enzyme were agitation, aeration, pH, and temperature and substrate concentration. The optimized values of variables were found to be agitation 300rpm, aeration 1.7 vvm, pH 7, temperature 300C and substrate concentration 2.09 %( w/v) respectively. At these values maximum alkaline protease specific activity was observed as 12.45 U/mg