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Paper Details

Population genetic differentiation of Heliotropium indicum as revealed by inter-simple sequence repeat (ISSR) analysis

*P. Benjamin Jeya Rathna Kumar and A. John De Britto

Journal Title:Journal of Chemical, Biological and physical sciences

Heliotropium indicum L. is an important traditional medicinal plant. Establishing the genetic diversity of the species would assist in its conservation and breeding program. Inter-simple sequence repeats (ISSR) markers were used to assess the genetic diversity and population genetic differentiation in 5 wild populations of H.indicum from southern regions of Tamil Nadu. ISSR analysis using six primers produced a total of 177 polymorphic bands. The number of polymorphic loci was 34 and the percentage of polymorphism was 79.07. The overall observed (na) and effective number of alleles (ne) was 1.7907 and 1.4770. Neis (1978) overall gene diversity (He) was 0.2870. The Shannons information index (I) was 0.4305. The genetic distance between the populations ranged from 0.1953 to 0.5034 and the genetic identity is ranged from 0.6045 to 0.8226. The similarity result indicates the presence of high level genetic diversity between populations and a dendrogram constructed by UPGMA method.