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Paper Details

High performance thin layer chromatographic determination of lornoxicam in human plasma

Shital S. Bhandari , Santosh V. Gandhi*, Padmanabh B. Deshpande, Navjot Ghobale

Journal Title:Journal of Chemical, Biological and physical sciences

A new simple High Performance Thin Layer Chromatographic (HPTLC) method for determination of Lornoxicam in human plasma has been developed and validated. The quantitative separation was achieved by using precoated silica gel 60 F254 plates as stationary phase and mobile phase consisting of Toluene: Acetic acid (8:2, v/v) with UV detection at 380 nm. Detection and quantitation were performed without using an internal standard. The calibration curve was linear over the range 20-800 ng/band. The limit of quantitation for Lornoxicam in human plasma was 10 ng/band and no interference was found from endogenous compounds. The recovery of Lornoxicam from human plasma was about 94.27 %. The method provides a direct estimate of the amount of Lornoxicam present in human plasma.