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Paper Details

Isolation, Purification and Characterization of Catalase from Aspergillus Species

Azad Chandrashekar.P

Journal Title:Journal of Chemical, Biological and physical sciences
Abstract


The catalase enzyme was purified to homogeneity from catalase has been purified from Aspergillus terreus and Aspergillus niger that were grown under submerged fermentation. Different purification steps (including Ammonium sulfate fractionation dialysis and column chromatography) were applied to the crude culture filtrate to obtain a pure enzyme preparation. In SDS PAGE, both enzymes migrated as polydisperse aggregates yielding broad and diffused bonds. These two fractions yielded one band corresponding to a molecular weight range from above 35 KDa (catalase). The enzyme exhibited distinctly under different pH and temperature profiles. The optimum pH and temperature for catalase were found to be 6 and 45C respectively. Hence the purified enzyme will be used in industrials applications as well as clinical trials.

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