Screening, isolation and identification of extracellular lipase producing bacteria from oil mill waste
Kalyana Chakravarthy Bonala, Lakshmi Narasu Mangamoori*
Journal Title:Journal of Chemical, Biological and physical sciences
Lipase-producing bacterial strains were isolated from soil by employing enrichment culture techniques.
Medium containing trybutryin agar was employed for isolation studies. Based on the radius of
lypolytic halo, one strain which has shown maximum lipase activity was coded as K7 and was selected
for further studies. The morphological, physiological and cultural characteristics of K7 were
determined according to Bergey's Manual of Determinative Bacteriology. 16S rRNA analysis of the
culture K7 also was carried out for phylogenetic analysis.