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Paper Details

Solid-State Fermentation and Characterization of Amylase from a Thermophilic Aspergillus niger Isolated from Municipal Compost Soil

Swati Kumari, Sourav Bhattacharya* and Arijit Das

Journal Title:Journal of Chemical, Biological and physical sciences

Screening of the six compost fungi for their ability to produce amylase using solid-state fermentation (SSF) revealed that Aspergillus niger was the best amylase producer. Among the several agronomic wastes, the highest yield of amylase from A. niger was obtained with rice bran. Soluble starch and peptone (1% w/w) when used as carbon and nitrogen supplements, respectively, enhanced the enzyme production. The optimum pH, temperature and incubation period for amylase production by the isolate were found to be 6.0, 30C and 120 h, respectively. The apparent molecular weight of the enzyme following sodium dodecyl sulphate polyacrylamide gel electrophoresis was found to be 55 kDa. The partially purified enzyme was optimally active at 70C and pH 5.0 and 9.0. The results obtained in the present study suggest that the A. niger isolated from the compost pile may act as a potent source for the industrial production of amylase. The enzyme being stable at elevated temperatures and under both acidic and alkaline pH can meet the requirements of food, beverage and detergent industries where often extremes of pH and temperatures are involved.