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Paper Details

Ex situ Biodegradation of Phenol by Native Bacterial Flora Isolated from Industrial Effluent

Sourav Bhattacharya*, Arijit Das and P. Nalini

Journal Title:Journal of Chemical, Biological and physical sciences

Phenol, a synthetically and naturally produced aromatic compound is one of the most widely used chemicals in the industrial processes. High solubility in water and the higher content in sewage make phenol a potential water pollutant. Due to its toxicity, phenol at higher levels reduces or even inhibits microbial population in effluents. A total of 28 bacterial forms were isolated from three industrial effluent samples of which, 3 isolates (A9, B5 and C3) showed considerable amount of growth and degradation potential at 200 mg/l concentration of phenol. Based on the 16S rDNA sequencing, A9, B5 and C3 were identified as Pseudomonas aeruginosa MTCC 1034, Pseudomonas fluorescens MTCC 2421 and Bacillus cereus ATCC 9634. The effects of parameters such as auxiliary carbon sources, nitrogen sources, pH and temperature on the growth and aerobic degradation potential of the isolates were evaluated. For all the isolates, the optimum conditions for growth and phenol degradation were 30C, pH 7.0, and at glucose and peptone concentration of 0.25 g/l. Within 48 hrs, P. aeruginosa MTCC 1034, P. fluorescens MTCC 2421 and B. cereus ATCC 9634 degraded 76%, 83% and 74% of phenol, respectively. Thus, based on their potential to aerobically degrade phenol at a higher concentration these native microorganisms can be cost effectively used in the bioremediation of the polluted sites.