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Paper Details

Screening of Antibiotic Producing Actinomycetes from Streams

J. A. Johnson1, T. Citarasu2, *P. A. Mary Helen3

Journal Title:Journal of Chemical, Biological and physical sciences

Actinomycetes were isolated from the leaf litter sample from streams by serial dilution agar plating method. The antimicrobial activity of actinomycetes extract was tested against pathogenic bacteria and fungi. The extract was tested against seven bacteria and five fungi. Then bioactive compounds were purified by silica gel column chromatography and it was identified by Gas Chromatography-Mass Spectrometry. Ten compounds such as 1H-Cyclopropa[a]naphthalene (11.199%), 2,4-Imidazolidinedione (12.238%), Diethylphthalate (13.494%), 1-Phenanthrenecarboxylic acid (23.825%), 10-Formamido-10,11- dihydro-2,3-dimethoxy (24.934%), Naphthalene (26.816%), 6-Methyl-1-pyridin-2-ylmethylene-1H,5H-f (27.334%), 1Beta,4Beta-Epoxy-6-Hydroxy-A-Homo-5,7,9 (29.227%), 1-Phenanthrenecarboxylic acid (29.943%), Bis(2-Ethylhexyl)phthalate (33.193%) were identified. Cytotoxic activity of actinomycetes extract on A375 cell lines were analysed by Trypan Blue dye exclusion method. The DNA of actinomycetes was isolated and the 16S rRNA gene was amplified using universal eubacterial 16S rRNA gene primers and it was phenotypically identified as Saccharothrix sp.