Effect of Inorganic Salts and Surfactants on the Production of ?-Amylase by a Mangrove Isolate of Aspergillus flavus using Solid-State Fermentation
Shilpi Bhardwaj1*, Vedamurthy A.B.1, Sourav Bhattacharya2 and Arijit Das2
Journal Title:Journal of Chemical, Biological and physical sciences
Filamentous fungi have been widely used for the production of amylases under solid-state
fermentation, wherein certain cultural parameters may provide good growth of microorganisms and
thereby better enzyme production. The effect of salts of certain metal ions on amylase production by
the mangrove isolate of Aspergillus flavus was studied. The highest yield of amylase production was
obtained by the addition of magnesium sulphate (0.1%) and calcium chloride (0.02%) respectively.
Supplementation of the enzyme production medium with non ionic surfactants in general and Tween
80 in particular resulted in an enhanced secretion of the starch hydrolyzing proteins in the medium.
Inoculum level is an important parameter for any fermentative operation. Optimum inoculum size was
found to be 10% spore suspension, beyond which enzyme production was seriously affected. The
results obtained in the present study suggest that the mangrove isolate of A. flavus may act as a
potent strain for industrial production of ?-amylase using SSF.