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Paper Details

Development and Validation of A HPLC-UV Method for Simultaneous Determination of Aromatic Fungicide Residues in Red Chilli Followed by Matrix Solid-Phase Dispersion

Tentu. Parvathamma, A. Ramesh* and Tentu. Nageswara Rao

Journal Title:Journal of Chemical, Biological and physical sciences

A simple, sensitive and inexpensive method was developed using matrix solid-phase dispersion (MSPD), together with high performance liquid chromatographic method for determination of aromatic fungicide residues (Chlorothalonil and Biphenyl) in red chilli. The evaluated parameters included the type and amount of sorbent (silica gel, C18 and neutral alumina) and the nature of eluent (ethyl acetate, dichloromethane and acetonitrile).The best results were obtained using 1.0 g of red chilli sample, 1.0 g of C18 as sorbent and 20ml of ethyl acetate-dichloromethane (1:1, (v/v)).The method was validated using in red chilli samples spiked with fungicides at different concentration levels (0.03 and 0.3 ?g/mL). Average recoveries (using each concentration six replicates) ranged 90-98%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.01-2.0 ?g/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.01 ?g/mL and 0.03 ?g/mL respectively.