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Paper Details

Sensitive Spectrophotometric Determination of Hg (II) in Different Water Samples, Soil Samples and Liver Samples by using 2, 4-Dimethoxybenzaldehydeisonicotinoylhydrazone (DMBHIH)

K. Ramakrishna Reddy*, N. Devanna and K.B. Chandrasekhar

Journal Title:Journal of Chemical, Biological and physical sciences
Abstract


A rapid, simple, sensitive and selective spectrophotometric method has been developed for the determination of Hg (II) using newly synthesized Chromogenic reagent 2,4-Dimethoxybenzaldehydeisonicotinoylhydrazone (DMBHIH). Hg(II) forms a Yellow coluored water-soluble complex with 2,4- Dimethoxybenzaldehyde isonicotinoylhydrazone (DMBHIH) in the pH range 6.0-10.0. The complex shows maximum absorbance at ?max 390 nm and in the pH range 7.0-9.0. At ?max 390 nm the complex shows maximum absorbance while the reagent blank shows negligible absorbance. Hence, analytical studies are carried out at ?max 390 nm and at pH 8.0 (Phosphate buffer) against reagent blank. Beer's law is obeyed in the range 0.5014-6.0177 mg/mL and the optimum concentration range obtained from ringbom plot is 1.0029-5.0147mg/mL of Hg(II). The molar absorptivity and Sandell's sensitivity for the coloured solution was found to be 2.8x104 L mol-1cm-1 and 0.00571 ?g.cm-2 respectively. The interference effect of various diverse ions has been studied. The complex shows 1:1 [Hg(II) : DMBHIH] stoichiometry with stability constant 4.5653x106 obtained from jobs method. The standard deviation of the method in the determination of 1.00295?g ml-1 of Hg(II) is 0.001 and the Relative standard deviation is 1.30 %. First and second order derivative spectroscopic method is developed at ?max 450 nm and 465 nm respectively for the determination of Hg (II), which is more sensitive than the zero order method. The developed method has been used for the determination of Hg (II) in different water samples, soil samples and liver samples. The results are in good agreement with the certified values.

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