Analytical Method Development and Validation of Lamivudin by Using Revers Phase HPLC
Manoranjan S*1, Sharada N1, Venkateswarlu G1, Sravan Prasad M2, Noorunnisa MD1
and Someshwar K1
Journal Title:Journal of Chemical, Biological and physical sciences
A simple, validated and sensitive HPLC method have been developed for the estimation of lamivudin in
bulk and in pharmaceutical formulations. Literature review reveals that analytical methods like ion
exchange chromatography, thin layer ion exchange chromatography and U.V are quite expensive when
compared to HPLC. Therefore the present study has been under taken in order to develop a new simple,
rapid, efficient and reproducible RP-HPLC method for analysis of lamivudin. After the optimization of the
HPLC method, validation of analytical method for the developed RP-HPLC method was done in accordance
with ICH guidelines. The %RSD was calculated to be 0.71 and having the correlation co-efficient 0.9996.
From the calibration curve constructed by plotting concentration Vs peak area, was found that there exist
a linear relationship in the concentration range of 50-150% for lamivudin.