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Paper Details

Effect of Orchidectomy and of Testosterone Administration upon Duodenal Ulceration Induced by Cysteamine

Ashokan Kannarath Valappil,

Journal Title:Journal of Chemical, Biological and physical sciences

Duodenal ulcers were induced in adult and old male mice using cysteamine hydrochloride under various experimental conditions. Under these experimental conditions, orchidectomized adult and normal old male mice strikingly decreased sensitivity to ulcer induction while testosterone propionate administration to orchidectomized adult mice showed an increased sensitivity to ulcer induction. This change in ulcer sensitivity reflected from histological, histochemical and biochemical studies. The histological study was performed by using haematoxylene-eosin staining technique. The histochemistry of the duodenal region was studied by using periodic acid Schiff reaction (PAS) for glycoprotein. The biochemical study was performed to study various constituents of glycoprotein like hexose, fucose, sialic acid and the protein from Brunners gland. The result showed that ulcer severity was more in orchidectomized- cysteamine treated mice and old mice treated with cysteamine. The histological studies showed that orchidectomy decreased or did not show any change in the ulcer sensitivity considering Cryptus Lieburkuhn and Brunners glands. The same result reflected by differential intensity in the staining property of the Brunners gland. The biochemical study showed that the glycoprotein contents were reduced many times in orchidectomized-cysteamine treated-testosterone administered adult and old male mice. These findings prove that testosterone of booth exogenous and endogenous augment the ulcer pathogenesis in cysteamine induced ulcer in male mice.