Production of Protease through SSF by Bacillus Subtilis NCIM 2724
Meena Vangalapati*, Sree Satya Nandam, Surya Prakash D.V and Sumanjali Avanigadda
Journal Title:Journal of Chemical, Biological and physical sciences
Production of protease employing the laboratory isolates of Bacillus sps. under solid state fermentation.
The present study was designed for study of increasing the production of protease feasible at commercial
level and an attempt has been made to optimize the different physico chemical parameters required for
obtaining the maximum production of protease using Bacillus subtilis and Bacillus lincheniformis. Bacillus
subtilis gives the maximum enzyme production by using papaya peel as the substrate with the optimized
conditions of incubation time 24hr, temperature 300C, moisture content 40%w/v, and inoculum level of
0.8%w/v and with substrate concentration of 10g and pH 8.0, glucose concentration 2.0%w/v. The
maximum production of protease enzyme considering all optimum conditions of various parameters was
found to be 0.69 mg/ml.