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Paper Details

Comparative Esterase Isozyme Variability in two Tephritid Fruit Flies, Bactrocera Dorsalis and B. Tau

Md. Abdur Rashid*, Tarana Siddika, Rowshan Ara Begum and Reza Md. Shahjahan

Journal Title:Journal of Chemical, Biological and physical sciences

Investigations were made on the comparative esterase variability of two tephritid fruit flies of economic importance (Bactrocera dorsalis and B. tau) using larvae, pupae and body parts from different age groups of adults of both sexes. Polyacrylamide gel electrophoresis (PAGE, 7.5%) was employed for this purpose, the gels were stained on ?- and ?- napthyl acetates. Altogether six esterase bands (Est-1, Est-2, Est-3, Est-4, Est-5 and Est-6) were detected in both B. dorsalis and B. tau. A remarkable variation was observed in the expression of esterase isozymes in both species. Expression of these isozymes increased with age in general, i.e., 2nd-, 3rd-instar larvae, pupae to adult on ward. This enzyme expression was also found to be increased from anterior part of the body to the posterior, in both species. Cypermethrin and malathion killed adult samples showed less isozyme expression compared to those of non-treated ones.