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Paper Details

Isolation, Characterization of Lipase Producing Bacteria from Crude Rice Bran Oil and Optimization studies by Response Surface Methodology (RSM)

B. Sumanjelin*, C.S.V. Ramachandra Rao, R. Satish Babu,

Journal Title:Journal of Chemical, Biological and physical sciences
Abstract


Lipases are enzymes that can be secreted by several micro organisms, making interesting the biodiversity exploration for searching new microorganisms able to produce these enzymes. Many agro-industrial residues can be used as potential substrates for production of enzymes. Lipolytic bacteria were isolated from crude rice bran oil and grown on tributyrin media containing 1 %( w/w) olive oil. The isolate showing maximum activity was identified by ABIS online method. RSM (response surface methodology) was used to develop the culture medium for Lipase production and central composite design (CCD) was used to optimize process variables (temperature, pH, salts, incubation period, inducers, carbon sources and nitrogen sources). Peak lipase activity was observed for beef extract as nitrogen source, olive oil as inducer, starch as carbon source at pH 7.0 and temperature at 25C.

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