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Paper Details

Studies on the Production of L-Asparaginase by Aspergillus terreus MTCC 1782 using Agro-Residues under Mixed Substrate Solid State Fermentation

J.V. Suresh* and K. Jaya Raju

Journal Title:Journal of Chemical, Biological and physical sciences

L-asparaginase has been used as anti tumour agent for the effective treatment of acute lymphoblastic leukaemia and as food processing aid to reduce the formation of acrylamide during frying of starchy foods at high temperature. L-asparaginase production under solid state fermentation was carried out by Aspergillus terreus MTCC 1782 using locally available agricultural residues. Among different agricultural materials (sesame oil cake, coconut oil cake, ground nut oil cake, wheat bran, rice bran, black gram husk, green gram husk) screened ,sesame oil cake (SOC) and black gram husk (BH) were selected and these two were mixed in different ratios for obtaining maximum enzyme activity . Out of ten combinations tested the ratio of 7:3 of SOC and BH supported maximum L-asparaginase production. The maximum yield (163.34 U/gds) of L-asparaginase by A.terreus was obtained using SOC and BH mixture of 60% initial moisture content, initial pH 7.0, supplemented with maltose (1.5%), ammonium sulphate (2.0%), and magnesium sulphate (0.1% w/v), inoculated with 1.5ml of 120h old fungal culture and incubated at 32OC for 120h. Both physico-chemical and nutritional parameters had played a significant role in the production of the enzyme L-asparaginase. Medium with optimized conditions and supplementation gave a maximum asparaginase activity of 163.34 U/gds which is above 2.4 fold and 10.2 fold increase in yield than that with medium of sesame oil cake (68.49 U/gds) and black gram husk (15.95 U/gds) alone.