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Paper Details

Genetic Diversity Analysis of Eri Silkworm by RAPD

M Shivashankar1, N Chandan1* and G S Nagananda2

Journal Title:Journal of Chemical, Biological and physical sciences
Abstract


Non-mulberry Erisilkworm, Samia cynthia ricini is an commercial exploitation of Vanya silk. To enhance the productivity and quality of silk fibers many attempts have been made through genetic manipulation. Since the information on the genetic basis, phenotypic variability and genetic diversity within populations of Eri silkworm is scanty, an attempt had been made to understand the genetic diversity among six populations viz., Yellow plain (YP), Yellow spotted (YS), Yellow Zebra (YZ), Blue Green Plain (BGP), Blue Green Spotted (BGS) and Blue Green Zebra (BGZ) by using Randomly Amplified Polymorphic DNA (RAPD) marker. Out of 20 RAPD primers screened, 18 primers generated good amplification of which 64 were polymorphic. RAPD profile generated for selected accessions with morphological dissimilarity showed 12% of polymorphism with primers ES-6, ES-8, ES-9 and ES-15. Cluster analysis (UPGMA) was generated to construct the dendogram. Jaccards similarity coefficients range (0.1 to 0.66) with maximum & minimum similarity noticed between Asc-05/Asc-06 and Asc-01/Asc-02. The range of similarity 0.1 to 0.66 indicated low genetic diversity in eri silkworm germplasm, which would be due to restricted environmental acclimatization.

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