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Paper Details

In Vitro Callus Induction from Stem Explants of Cissus Quadrangularis L. (Hadjod)

* Teware Kalpana 1. Singh Pratibha 2 and Mehta Rakesh 3

Journal Title:Journal of Chemical, Biological and physical sciences

Stem explant of Cissus quadrangularis L., belongs to vitaceae family were cultured on MS media supplemented with 2,4D, NAA, IBA. Callus formation on the surface of stems on high and low concentrations of NAA alone and combined with BAP, stimulated the callus induction. However, concentrations of IBA used here, inhibited Shoot induction. Induced Callus is used for the study of secondary metabolite. Within 90 to 120 days of the culture it showed growth up to 90 %. Aim of this study is to enhance the phytochemical after applying tissue culture technique.