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Paper Details

Comparison of microscopic, macromorphological and aflatoxin producing capabilities of Aspergillus species associated with rhizosphere of groundnut (A. hypogaea L.)

Manjusha Chakranarayan*and Anita Pati

Journal Title:Journal of Chemical, Biological and physical sciences
Abstract


The role of laboratory in the identification has gained greater attention as the incidence of fungal infection is increased. The objective of the study was to isolate the aflatoxin producing Aspergillus species from the rhizosphere of groundnut. Identification of the Aspergillus species was done on the basis of morphological methods including; microscopic and macroscopic characteristics. Ten standard species of Aspergillus namely A. flavus, A. parasiticus, A. niger, A. repens, A. fumigatus, A. kanagawaensis, A. ochraceus, A. sydowi, A. terreus and A. nidulans and were isolated and characterized. Rapid detection of aflatoxin production was carried out by using coconut agar medium. The colonies were detected under long-wave UV light by the blue-green fluorescence on the reverse side after 2 to 5 days of growth. Further the aflatoxin production capacity was screened using thin layer chromatographic technique. Depending upon the intensity of bands on TLC plates A. flavus and A. parasiticus were found to be highly aflatoxigenic and produce aflatoxin B1 on Richard liquid medium in the range of 966.66-266.66 ?g/kg respectively. Quantitation of aflatoxin was done by using quantitative TLC, which was based on the comparison of the fluorescent intensity of the sample spot with standard spot. While all the other species are non-aflatoxigenic strains.

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