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Paper Details

A new analytical method development and validation for estimation of ciproflaxacin and metronidazole byiso absorption method by using UV spectrophotometer

Natesh.G*1, MD.Azeez2, Manoranjan Sabat2, Goli.Venkatehwarlu2, Nazia Begum2 and A.Srivani2

Journal Title:Journal of Chemical, Biological and physical sciences

A simple, validated and sensitive UV method has been developed for the estimation of ciprofloxacin and metronidazole. Literature review reveals that analytical methods like ion exchange chromatography, thin layer ion exchange chromatography are quite expensive comparative UV method .therefore present study has been under taken to develop an efficient UV method like Q- absorbance for the analysis of ciprofloxacin and metronidazole. After optimization of UV method, estimation and validation of method were done according to ICH guidelines. % RSD is found to be 0.601 and correlation coefficient were found to be 0.997 for metronidazole and 0.994 for forciprofloxacin .the?max of ciprofloxacin and metronidazole are determined as 279nm and 313nm. From the overlay spectra the iso -absorptive point is obtained as 290nm .for estimation 279nm and 290nm are considered for calculation . from the standard graph it was revealed that the beer, s limit for both drugs follow the concentration range of 2-10?g/ml. this procedure was applied successfully for analysisof ciprofloxacin and metronidazole without prior separation and without interference of excipients.