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Paper Details

Recent Advances in Banana Improvement through Biotechnological Approaches

Babita, H.S. Rattanpal* and Kishore Kumar Thakur

Journal Title:Journal of Chemical, Biological and physical sciences
Abstract


Bananas and plantains (Musa spp.) are the most important tropical fruit crops. The major constraints to banana and plantain production are fungal diseases (black Sigatoka and Fusarium wilt), pests (banana weevil and nematodes) and viruses. The development of resistant cultivars is considered to be the most effective approach in controlling diseases and pests. The development of tissue-culture techniques has ensured production of cleans (pest and disease-free) planting material and also establishes faster, grow more vigorously, have a shorter production cycle and produce higher yield. Tissue culture is an excellent mechanism for promoting banana conservation and germplasm distribution since most cultivars are seedless. Embryo rescue, involving the excision and culture of developing zygotes, represents the technology that most easily assists conventional breeding. Several molecularmarker techniques have been applied to banana, including genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), restriction fragment length polymorphism (RFLP), polymorphisms based on the variable number of tandem repeats (VNTR), microsatellite DNA, RAPD, amplified fragment length polymorphism (AFLP) and simple sequence repeats (SSR). Agro bacterium-mediated transformation offers several advantages over direct gene transfer methodologies like particle bombardment, electroporation and marker-assisted selection has become a reliable method to improve disease resistance in Musa. It can therefore be concluded that biotechnology has great potential for the

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