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Production and optimization of L-Glutaminase (EC.3.5.1.2) by serratia marcescens using wheat bran under statistical designs

Production and optimization of L-Glutaminase (EC.3.5.1.2) by serratia marcescens using wheat bran under statistical designs

Journal Title:Journal of Chemical, Biological and physical sciences
Abstract


L-Glutaminase majorly produced by micro organism including bacteria, yeast and fungi. L-Glutaminase mainly catalyzes the hydrolysis of ?-amido bond of L-Glutamine. In this report, Optimization of the culture medium for L-Glutaminase production using serratia marcescens was carried out. The optimization of L-Glutaminase production using Wheat bran as substrate was performed with statistical methodology based on experimental designs. The screening of ten nutrients for their influence with Wheat bran on L-Glutaminase production is achieved using Plackett-Burman design. The basal medium contained Peptone 30 g/L, Manganous sulphate 0.7 g/L, K2HPO4 0.7 g/L, NaCl 9 g/L was selected based on their higher influence on L-Glutaminase production. After medium components optimization, the Temperature, pH, Time, composition of the Wheat bran, and Inoculum size was optimized using Response Surface Methodology (RSM). The predicted optimum levels are as follows: Temperature 28.43 0C, pH 6.07, Time 67.96 h, wheat bran 26 g/L and Inoculum size 1.30 %. This medium components and parameters were projected theoretically to produce an L-Glutaminase activity of 2299.96 IU/ml. Theused methodology was validated using this optimized media components and parameters; the L-Glutaminase activity 2285 IU/ml was obtained.

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