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Paper Details

Evaluation of Beneficial Effects of Medicago Sativa (Alfalfa) In Iron-Overload Conditions

Rinal Patel*and Pravin Tirgar

Journal Title:Journal of Chemical, Biological and physical sciences

In medicine, iron overload indicates accumulation of iron in body from any cause. The most important causes are thalassemia, hereditary haemochromatosis (HHC) and transfusional iron overload, which can result from repeated blood transfusion. Aim of present study is to investigate in-vivo iron chelating potential and beneficial effects of Medicago sativa in iron overload and its complications. Iron chelating and organo protective activity of methanolic (250 mg/kg) and water (500 mg/kg) extracts of Medicago sativa along with standard drug desferoxamine were assessed against irondextran induced iron overload models in Wistar rats which results in condition of chronic iron overload found in thalassemia patients. At the end of 15 and 30 days of in-vivo trial, serum iron and ferritin , urine and fecal iron levels including complications on vital organ by histopathological study and test for biomarkers were (SGPT, SGOT, Serum creatinine, creatine kinase) were estimated to measure organo protective effects. There were significant decreased in serum iron, ferritin were observed compared to iron overloaded rats. These beneficial effects were observed because methanol and water extracts of M. sativa increased excretion of iron in urine and fecal due to iron chelation property of extract. Organo-protective effects on liver, heart and kidney of both extracts of M. sativa in iron overloaded rats were confirmed by histopathological study and reduction in various markers like SGPT, SGOT, creatinine, creatinine kinase levels. Data of our study confirmed that methanol and water extracts of Medicago sativa have significant iron chelating activity and organo-protective effects in iron overload conditions which is very beneficial in management of iron overload disorders like thalassemia, haemochromatosis like conditions.