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Paper Details

Invitro culture of Stevia rebaudiana var Bertoni

Patil, N.M.

Journal Title:Journal of Chemical, Biological and physical sciences

The effect of different plant growth regulators on explants was studied by In vitro culture technique in Stevia rebaudiana var Bertoni, an important medicinal, zerocalorie value, sweet tasting and antidiabetic herb. For effective shoot initiation, nodal segments were cultured on Murashige and Skoogs (MS) medium supplemented with varying plant growth regulator combinations. The control was compared with the medium supplemented with different concentrations of NAA and BAP for shoot induction and 2, 4-D and IAA for callus induction. The best results were obtained from MS medium supplemented with BAP and NAA at the concentrations of 2.0 mg/l and 0.5 mg/l for shoot initiation and 2.5mg/l and 1.5 mg/l 2,4-D and IAA for callus induction respectively. Further study on development of plantlets invitro and their hardening are in progress.