Potentiometry and Spectrophotometry Studies of Binary Complex of Tripeptide TFA.Leu-Ala-Phe- OH with Cu (II).
Sanjay N. Aher,a,b,*, Supriya S. Aher b, S. D. Rathod a.
Journal Title:Journal of Chemical, Biological and physical sciences
Tripeptide TFA.Leu-Ala-Phe-OH synthesized by using conventional
solution phase peptide synthesis strategies. The proton and Cu (II) solution
equilibrium investigation of tripeptide binary complex are studied by potentiometric
and spectrophotometric measurements in aqueous media. Measurements were carried
out at varying temperature and ionic strength conditions. Potentiometric studies
performed by using Calvin-Bjerrum titration technique as used by Irving and Rossotti
while spectrophotometric measurements performed to determine conditional stability
constant (Log Kcond) by Jobs continuous variation method at pH 5.5. Both
potentiometric and spectrophotometric data evaluation shows inverse trend with
varying temperature and ionic strength conditions on the proton and Cu (II) binary
complex stabilities in aqueous media.