Stability Indicating RP-HPLC Method for Determination of Atenolol and Amlodipine Besylate in Tablets
Milind Ubale1, Sayyed Husain2 and Vilas Chaudhri3.
Journal Title:Journal of Chemical, Biological and physical sciences
A reverse phase method has been developed for the quantitative estimation of
Atenolol and Amlodipine Besylate in Tablets. The Quantification was carried out using RP
stainless steel column ODS C18 250 x 4.6 x 5 ? L1 packing in Isocratic mode with mobile
phase containing 0.03 M Potassium buffer: Acetonitrile in the ratio of 50:50. pH 3.4
adjusted with ortho-phosphoric acid. Solution degassed before use. Flow rate maintained at
1.0 ml/minute and the detection wavelength was set at 237 nm. The linearity was found to
be in the range of 16-22 ?g/ml for Atenolol and 8 to 12 ?g/ml for Amlodipine Besylate .The
proposed method was found to be simple, precise, accurate, and reproducible for the
estimation of Atenolol and Amlodipine Besylate.