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Paper Details

Stability Indicating RP-HPLC Method for Determination of Atenolol and Amlodipine Besylate in Tablets

Milind Ubale1, Sayyed Husain2 and Vilas Chaudhri3.

Journal Title:Journal of Chemical, Biological and physical sciences
Abstract


A reverse phase method has been developed for the quantitative estimation of Atenolol and Amlodipine Besylate in Tablets. The Quantification was carried out using RP stainless steel column ODS C18 250 x 4.6 x 5 ? L1 packing in Isocratic mode with mobile phase containing 0.03 M Potassium buffer: Acetonitrile in the ratio of 50:50. pH 3.4 adjusted with ortho-phosphoric acid. Solution degassed before use. Flow rate maintained at 1.0 ml/minute and the detection wavelength was set at 237 nm. The linearity was found to be in the range of 16-22 ?g/ml for Atenolol and 8 to 12 ?g/ml for Amlodipine Besylate .The proposed method was found to be simple, precise, accurate, and reproducible for the estimation of Atenolol and Amlodipine Besylate.

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