Optimization of dispersive liquid-liquid micro extraction followed by HPLC-DAD determination of Cr (III) and Co (II) based on the complexation reaction with morpholin -4-carodithioate

A.Chehrehghania, N. Samadia* , M. R. Vardast

Journal Title:Journal of Chemical, Biological and physical sciences
Abstract

In this study, a dispersive liquid-liquid microextraction (DLLME) method was used for preconcentration of Cr (III) and Co (II) cations in water samples. Morpholin -4- carodithioate (MDTC) was used as chelating agent prior to extraction. High-performance liquid chromatography-diode array detection (HPLC-DAD) was used for quantification of the analytes after preconcentration. The effects of extraction, nature and volume of disperser solvent, pH value of sample solution, extraction time and extraction temperature were investigated. Under the optimum conditions, the calibration graphs were linear in the range of (10-4000?g L-1) and (5-4000 ?gL-1) with detection limits of 3 ?g L-1 and 3 ?g L -1 for Co(II) and Cr(III) respectively. Recoveries and enrichment factures for Cobalt and Chromium were obtained (90%, 63.69) and (84%, 58.42), respectively. The DLLMEHPLC-DAD method was successfully applied to the analysis of Cr (III) and Co (II) in aqueous samples.

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