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Paper Details

Alterations in Antioxidant Enzymes Levels Following Sub Chronic Exposure of Sodium Arsenite in Female Rats

Madhuri Mehta and S. S. Hundal

Journal Title:Journal of Chemical, Biological and physical sciences

The present work was undertaken to assess the sub chronic effect of sodium arsenite on antioxidative potential of lungs, brain and spleen of female albino rats. Forty eight female rats were divided in to 4 groups consisting of 12 animals each. Group I animals received distilled water throughout the experiment and served as control. Group II, III and IV animals received sodium arsenite at the doses of 10?g/L, 30?g/L and 50?g/L dissolved in distilled water ad libitum for 60 days. Half of the animals from each group were sacrificed after 30 days of sodium arsenite exposure and remaining half after 60 days under mild anaesthesia. Lungs, spleen and brain were collected, weighed, minced and homogenized for the estimation of antioxidant enzymes viz. superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), reduced glutathione (GSH) and estimation of lipid peroxidation (LPO), a marker for oxidative stress. A significant decline in the activity of SOD, CAT, GST and GSH in all tissues at different time periods (30 and 60 days) has been detected while the levels of LPO were found to increase significantly in treated animals as compared to control. In conclusion, we may infer that exposure to sodium arsenite decreased the antioxidative potential of lungs, spleen and brain, making them susceptible for various diseases due to generation of oxidative stress.