A Study on Biochemical and Molecular Methods for Diagnosis of Pulmonary Tuberculosis
Pratibha Rani Dwivedi1, Stuti shriwastava1, Seema Tiwari1*, Rashmi Arnold2,
Arti Saxena3 and Umesh Pandey3
Journal Title:Journal of Chemical, Biological and physical sciences
TB is most common opportunistic disease that affects people infected with
HIV. As HIV debilitates the immune system, vulnerability to TB is increased.
Recently, new multi drug-resist strains of tuberculosis (MDR-TB) have developed and
are spreading. A multidrug resistance strain is defined as M. tuberculosis resistant to
ionized and rifampin with or without resistant to other drugs. This has resulted is
many case of magically treatable, and often fatal diseases. Inadequate therapy is the
most common means by which resistant bacteria are acquired and patients who have
previously undergone therapy should be presumed to harbor MDR-TB, until proved
otherwise. The objective of this study was to estimate the level of certain
pathologically important isozyme (eg LDH) in cases (TB patients) and controls and to
standardize the PCR based identification method for mycobacterial strains in DNA
samples extracted from sputum specimen.