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Paper Details

Growth and biological production of polyhydroxy butyrate (PHB) by bacillus megaterium MTCC 8075

R. Muralidharan* and K.V.Radha

Journal Title:Journal of Chemical, Biological and physical sciences
Abstract


In this study, a two-stage cultivation strategy was adopted for the production of Poly-?- hydroxy Butyrate (PHB) using Bacillus megaterium MTCC 8075. The culture conditions for PHB production by Bacillus subtilis MTCC 9763 were optimized with different carbon, Nitrogen sources, temperature, pH and Incubation time. PHB production was attained in the presence of glucose as carbon source with a yield of 55.0 mg PHB/g cell weight and peptone as nitrogen source with a yield of 17.2mg/g cell weight. The optimum pH was found to be 7. A maximum yield of 35.46mg PHB/g cell weight corresponding to a temperature of 35C was obtained and at an incubation time of 72 hours (52.2mg/g cell weight). PHB was characterized using Infra-Red (IR) spectroscopy.

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