The Effects of Allogeneic cADSCs on an Experimental Ear Auricular Defect to Evaluate Cartilage Regeneration in a Canine Model
Mahdieh Ghiasi1*, Sahar Farzaneh2, Mohammad Bigdelo3, Mehran Vosoogh4
Journal Title: Journal of Clinical Medical Research
Objective: Ear is one of the most important organs of the auditory system and may fail as a result of trauma, tumors, or other diseases. Allograft transplantation of adult stem cells has been used as an excellent procedure for restoring damage from different diseases. The aim of this study is to assess the regenerative ability of adipose-derived stem cells in ear auricle cartilage defects in a dog model injected with different treatments.
Materials and Methods: The adipose-derived stem cells were isolated from canine adipose tissue. After cultured in medium, these cells were obtained after four passages. This study evaluated into three groups: Groups 1 and 2 were determined by mixing of the adipose-derived stem cells with fibrin glue biomaterial and without fibrin glue, respectively. The mixing of the adipose-derived stem cells with fibrin glue biomaterial and without fibrin glue were separately injected into the mid-portion of a surgically provided canine auricular cartilage injury. After two months, the auricular defect evaluated histologically.
Results: Our findings demonstrated that points of new cartilage were formed at the location of the surgically stimulated defect. Four months after the surgical injection, it was shown that the defect was completely ameliorated with mature and native cartilage tissues. Also, there was no observed formation of cartilage tissue in the control group.
Conclusion: Our results clearly indicate that allogeneic adipose-derived stem cells implantation into the experimental ear auricle defect is a safe, effective and relatively simple therapy of ear lesion in dogs, with a significant improvement of ear cartilage regenerative.