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Development and Validation of Lercanidipine Hydrochloride and Atenolol by Using RP-HPLC and UV Spectroscopy

Priyadarshani S. Bansode, Mahendra Singh Ranawat, Ravindra Kamble, Chetan Singh Chauhan

Journal Title:Indian Journal of Pharmacy and Pharmacology
Abstract


Lercanidipine hydrochloride (LCD), a dihydropyridine calcium channel blocker is used in the treatment of hypertension. Atenolol (ATN), a cardio selective ?-blocker is used as antihypertensive, antianginal and antiarrhythmic. The proposed method was development and validation of UV-Spectrophotometric and RP-HPLC for determination in bulk and tablet dosage form. In UV Spectroscopic method, LCD showed ?max at 237 nm in 50% v/v acetonitrile. The RP-HPLC analysis was performed by using a mobile phase of methanol: water (95:5 v/v), at a flow rate of 1.0 ml/min. By comparing validation results of both the methods, UV-spectrophotometric method is found to be simple, economical and rapid and RP-HPLC method were found to more precise, accurate, rugged and robust.

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