Antioxidants (vitamin E and gallic acid) as valuable protective factors against myocardial infarction
Mohamad Sayyed Bakheet, Sameh Soltan, Ahmad Gadalla, Haredy Hassan Haredy, Maher Abdel Shakoor
Journal Title:Basic Research Journal of Medicine and Clinical Science
Myocardial infarction is acute condition of necrosis of the myocardium that occurs as a result of imbalance between coronary blood supply and myocardial demand. It is the leading cause of death in high or middle income countries and second only to lower respiratory infections in lower income countries. Reactive oxygen species participate in normal cell signaling as mediators that regulate vascular function. In the vascular wall, ROS are produced by all layers including endothelium, smooth muscle and adventitia. Under physiological condition, ROS are produced in law concentrations and act as signaling molecule that regulate VSMC contraction and relaxation, and participate in VSMC growth under pathophysiological conditions, these free radicals play important role in various conditions, including atherosclerosis, ischemic heart diseases, arrhythmias, cardiomyopathy and congestive heart failure. So, pharmacological studies in the present work were done to evaluate the protective effects of different antioxidants (vit. E and gallic acid) on cardiac marker enzymes (AST, ALT, CK and LDH), CTnI, lipid peroxidation and antioxidant parameters in isoprenaline-induced MI in rats. In this study fifty six adult male albino rats were divided into four groups (14 rats each), Group I: Negative control rats were subcutaneously injected with saline twice at an interval of 24 hours. Group II: positive control rats were subcutaneously injected with ISO (100 mg/kg) twice at an interval of 24 hours, it serves as control for group III and IV. Group III: rats were pretreated with gallic acid (15mg/kg) once daily orally for 10 days and then subcutaneously injected with ISO at an interval of 24 h for 2 days. Group IV: rats were pretreated with vitamin E (100 mg/kg) once daily orally for 30 days and then subcutaneously injected with ISO at an interval of 24 h for 2 days. At the end of the experimental period, blood samples was collected for estimation of cardiac marker enzymes, hearts were homogenized for the assay of MDA, SOD, GSH and histopathological examinations were done. The results of the present study showed that: 1- Prior treatment with vitamin E and gallic acid significantly decrease the activity of serum cardic marker enzymes in ISO induced MI in rats: 2- Prior treatment with vitamin E and gallic acid significantly increase the levels of antioxidants in ISO induced MI in rats: 3- Prior treatment with vitamin E and gallic acid significantly decrease the level of lipid peroxidation in ISO induced MI in rats. So, the use of antioxidants (vit. E and gallic acid) have a valuable protective role against MI through overcoming free radicals.
Keywords: Myocardial infarction, lipid peroxidation, antioxidants, vitamin E and gallic acid