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Paper Details

Responses of human osteoblastic cells to natural zeolite biomimetic coat on nanoporous titania plate

Sahar A.Fadlallah, H. F. Yousef, Q. Mohsen and Nahla S. El-Shanawy

Journal Title:Basic Research Journal of Medicine and Clinical Science

Previously, natural zeolite coat/ nanoporous titania (ZNT) plates has been shown to induce osteoblast differentiation in rabbit bone marrow cells in our lab. The present study investigated the effect of continuous immersion of different titanium (Ti) plate treatment on osteoblast differentiation of human osteoblast-like cells (HOB). Primary culture and first passage were cultured in media with or without plates. During the culture period, cells were incubated at 37 C in humidified atmosphere of 5% CO2 and 95% air. Cell proliferation, cell viability, total protein content and alkaline phosphatase (ALP) activity were evaluated at 7, 14, and 21 days. The different plates did not affect cell viability and total protein content, but reduced cell proliferation. ALP activity was increased by the end of experiment in cell culture with ZNT in comparison to the other groups. The results indicate that, for HOB, in the ZNT appear to be required for development of the osteoblast phenotype and did not interfere with osteoblast differentiation expressed by reduced cell proliferation and increased ALP activity. Keywords: Natural zeolite; Nanoporous titania; Cell proliferation; Osteogenic markers.