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Paper Details

In Silico Analysis of Amino acid Substitutions in DNA gyrase subunit A

Payam Behzadi, Elham Behzadi, Mehrdad Moosazadeh Moghaddam, Ali Najafi, Reza Ranjbar

Journal Title:Journal of Applied Biotechnology Reports
Abstract


The broad consumption of antibiotics such as fluoroquinolones and the genetic adaption of opportunistic pathogenic bacteria including Pseudomonas aeruginosa, has led to develop of floroquinolone-resistant strains of Pseudomonas aeruginosa. Thus, the aim of the present study is to reveal the types of hot spot mutations occurring in selected DNA gyrase subunit A gene in fluoroquinolone-resistant Pseudomonas aeruginosa strains. For this purpose, 70 fluoroquinolone-resistant partial cds genes of Pseudomonas aeruginosa TOHO strains (P12-P377) for DNA gyrase subunit A have been aligned with wild genes for DNA gyrase subunit A by BLAST program and multiple alignment, to detect probable gyrA mutations. Based on analyses hot spot mutations including Thr-83?Ile (92.44%), Ile-83?Thr (1.33%), Asn-87?Asp (0.44%) existing as single and Thr-83?Ile & Asn-87?Asp (5.78%) as double substitutions were detected. According to this survey, the point mutations in DNA gyrase subunit A gene need more consideration about fluroquinolone-resistant Pseudomonas aeruginosa isolates. The strains with hot spot SNP mutations may rise a huge concern from epidemiologic aspect for antibiotic therapy treatment.

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