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Paper Details

Extraction, identification and quantitative HPLC analysis of flavonoids from fruit extracts of Arbutus unedo L. from Tiaret area (Western Algeria)

Khadidja Bouzid1, Fouzia Toumi Benali, Rabah Chadli, Mohamed Bouzouina, Aman Bouzid, Amal Benchohra, Mustapha Mahmoud Dif, Samia Bouzid

Journal Title:Basic Research Journal of Agricultural Science and Review

The aim of the current study was to evaluate the total phenolic, flavonoid content and to investigate the antioxidant capacities of the fruit extracts of Arbutus unedo L. that grows in Tiaret area (Western Algeria). First we have extracted the fruit by some non-polar solvent (chloroform, ethyl acetate, 1-butanol). Total phenolic content and total flavonoid content were evaluated according to the Folin-Ciocalteu procedure, and a colorimetric method, respectively. Extracts content was determined by using a high-performance liquid chromatography (HPLC)-UV method. The total phenolic contents of A.unedo L. varied between 12.750.06 to 34.171.36 mg gallic acid equivalent/g of dry weight of extract. The total flavonoid varied from 2.180.10 to 6.541.14 mg catechin equivalent/g. The antioxidant potential of all extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, the IC50 of acetate ethyl was the best by 0,009 mg/ml may due to the phenolic compound. The antioxidant activity of all extracts was better than ascorbic acid. The extract obtained under optimum conditions was analyzed by HPLC and five flavonoid compounds were identified; they are catechin, apiginin, silybin, fisetine and naringin. Keywords: antioxidant activity, Arbutus unedo L., fruit, flavonoids, phenols, HPLC, Western Algeria.