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Paper Details

Antibiogram of bacterial species causing skin wound infections

Mahesh Raj Pant, Dipti Shrestha, Shovana Thapa

Journal Title:Novel Research in Microbiology Journal

       Wounds occur when the integrity of any tissues is compromised. Infection causes significant increase in costs, morbidity, and potential mortality. This study was conducted during the period from July, 2015 to January, 2016 with the aims of identifying the etiological agents causing skin wound infections, and their antibiotic susceptibility profile among patients visiting International Friendship Children’s Hospital (IFCH), Maharajgunj, Kathmandu, Nepal. Specimens were processed by conventional culture technique and antibiogram of isolates were done by modified Kirby-Bauer disc-diffusion method. Out of 219 skin pus samples, 132 (60.3%) were reported to be bacterial culture positive. Eight different bacterial species were identified as; S. aureus 75 (56.8%), Coagulase negative S. aureus (CONS) 20 (15.2%), Escherichia coli 13 (9.8%), Citrobacter spp. 7 (5.3%), Pseudomonas aeruginosa 5 (3.7%), Klebsiella spp. 5 (3.7%), Proteus spp. 5 (3.7%) and Enterobacter spp. 2 (1.5%), all were isolated from culture positive specimens. Antibiotic susceptibility test (AST) of all Gram-negative isolates showed that Colistin and Imepenum were the most effective antibacterial drugs. Out of total 75 S. aureus isolates, all were reported to be susceptible to Vancomycin, whereas, 23 (30.7%) were resistant to methicillin. This study reported that S. aureus strains were the predominant isolates. Prevalence of multi-drug resistant strains of S. aureus is increasing. Current results demonstrated that antibiotic resistance in Gram-positive and Gram-negative bacteria is increasing in alarming trends that lead to failure of treatment.