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Paper Details

Molecular Characterisation of ESBL producer E. coli

Abdulghani M. Alsamarai, Tikrit University College of Medicine Shler Ali Khorshed, College of Education, Kirkuk Education Authority. Imad Jihad Weli, Kirkuk General Hospital, Kirkuk Health Authority. Correspondence author: Shler Ali Khorshed, Open College of Education, Kirkuk Education Authority

Journal Title:Aalborg Academy Journal of Medical Sciences
Abstract


Background: Antibiotic resistance emerged as clinical problem challenge the effective treatment of infections. Virulence factor may play an important role in the influence of antimicrobial resistance. Objective: To determine the frequency of resistance gene in E. coli clinical isolates from women with urinary tract infection. Materials and Methods: Fifteen E.coli clinical isolates were tested by PCR to determine their molecular characterization. Results: The bla CTX –M gene was not detected in 6.7% out of the tested 15 E. coli clinical isolates from women with urinary tract infection. However, bla OXA gene was detected in all E. coli tested clinical isolates from pregnant women, female student and diabetic women with urinary tract infection. While bla TEM gene and bla SHV gene were not detected in 33.3% and 40% out of the tested E. coli clinical isolates respectively. Conclusions: Four types of ESBL genes were detected, and shows new trend of distribution, which indicated the predominance of OXA and CTX-M genes. Key words: Urinary tract infection, Resistance, PCR, , CTX, OXA, OXA, SHV.

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