Protective Effects of Turmeric Extracts Against CCl4 -Induced Liver Injury in Rats
Firdaws A. AL-Mashhadani, Deparetment of Medical Laboratory Techniques, College of Medical Technology, AL-Kitab University, Kirkuk, Iraq.
Essam Salim Rasheed, Department of Medical Laboratory Techniques, College of Medical Technology, AL-Kitab University, Kirkuk, Iraq.
Nazdar Nabee Wasman, Department of Food Technology, Agriculture College, Salahaddin University, Erbil, Iraq.
Corresponding author: Firdaws A. AL-Mashhadani, Dep. Of Medical Lab. Techniques, College of medical technology, AL-Kitab University, Kirkuk, Iraq.
Journal Title:Aalborg Academy Journal of Medical Sciences
Background: This study highlights the effect of turmeric extracts on CCl4-induced liver injury by follow up the hematological and biochemical parameter in treated rat group. Objective: Estimate the antioxidant activity of Curcumin and its hepatic protective activity, also to assign the oxidative stress, and antioxidant markers and some hematological parameters in CCl4 treated rat groups. Besides that, this study aims to evaluate the effect of curcumin extract against various pathogenic bacteria growth. Materials and methods: three groups of rats were used, each group having ten albino rats. The first group represent the control group, which received a normal diet and intraperitoneal injection with oil (0.5 ml/kg b.w. (body weight)). The second group represented the CCl4 1ml/kg b.w. (1:1 in olive oil) model. The third group received CCl4 1 ml/kg body weight (1:1 in olive oil) and turmeric 200 mg dissolved in water and given to rates by gavage for four weeks (March to April 2015). Results: The model produced oxidative stress and arising in the level of AST, ALP (Aspartate aminotransferase, Alanine aminotransferase respectively), and direct bilirubin and lowering GSH (glutathione), liver SOD (superoxide dismutase) levels. The treated group showed a significant lowering of AST, ALT, ALP (alkaline phosphatase), direct bilirubin and increasing GSH, liver SOD levels. Also ameliorated inflammation caused by CCl4 treatment via decreasing WBC (White Blood Cells), RBC (Red Blood Cells), and PLT (Platelets). These results referred to a good evidence, that is, turmeric could be used as a source of antioxidant, hepatic protective activities and a good antibiotic agent against some pathogenic bacteria. The watery extracts of Turmeric powder with different concentrations in ml inhibited the growth of the pathogenic E. coli, Staphylococcus aureus, Bacillus subtillus. Conclusions: Turmeric extracts was protective against oxidative damage in CCl4 induced liver injury in rats. Additionally, the alcoholic turmeric extract was with higher antimicrobial activity than watery extract. Keywords: Turmeric, CCl4 – Induced liver injury