Development and Validation of Analytical Method for Simultaneous Estimation of Cilnidipine and Chlorthalidone in their Combined Dosage Form
Mayank Bapna, Bhoi Khushbu Girishbhai
Journal Title:International Journal of Ayurveda and Pharmaceutical Chemistry
A simple, rapid and precise reverse phase high performance liquid chromatographic method was developed for the Simultaneous Estimation of Cilnidipine and Chlorthalidone in their combined dosage form. The chromatographic separation was achieved on Zorbax Bonus RP (250×4.6) mm, 5 µ column with an isocratic mixture of 0.05 M KH2PO4 Buffer (pH 6.5) : Methanol in the ratio of 50:50 v/v, respectively. The mobile phase was kept at a flow rate of 1.0 ml/min with injection volume of 20μl and wavelength of detection 225nm at room temperature. The retention times for Chlorthalidone and Cilnidipine was found to be 8.107±0.1min and 4.337±0.1min, respectively. The linearity was obtained in the range of 50-150μg/ml for both Chlorthalidone and Cilnidipine with correlation coefficient 0.9993 and 0.9996, respectively. The proposed method was found to be linear, accurate, precise, stable, robust and specific and was successfully applied for the determination of investigated drugs in combined dosage form.