Liquid-liquid extraction by low-temperature partition and high-performanceliquid chromatography for determination of flunixin and phenylbutazone in horse hair.
Fernando Luciano Alves de Souza, Andréa Alice da Fonseca Oliveira, Ana Paula Silveira Paim
Journal Title:Revista Agraria Academica / Agrarian Academic Journal
This work proposes the determination of flunixin and phenylbutazone in horse hair using liquid-liquid extraction by low-temperature partition (LLE-LTP) and HPLC-UV detection. The method efficiently extracted the analytes and purified the extract in a single step. The method has never been applied for horse hair and showed to be easy to perform with satisfactoryresults. Limits of detection and quantification were 0.012; 0.024 ng mg-1for flunixin and 0.036; 0.073 ng mg-1for phenylbutazone, respectively. Nine out of 14 samples analyzed showed positive results for at least one analyte. The average recoveries for flunixin and phenylbutazone were above 97.0 and 92.0 %, respectively.