Callus induction from leaf explants and stem nodes of Cumari-do-Pará (Capsicum chinense Jacquin)
Mariane Rabelo Coelho Fernandes, Bruno Henrique Gomes, Ana Paula Oliveira Nogueira
Journal Title:Revista Agraria Academica / Agrarian Academic Journal
Considering the importance of the production of pepper species, tissue culture techniques become important for large-scale seedling production and for the production and exploitation of secondary metabolites with pharmacological activities and as natural herbicides. The aim of this work was to evaluate the induction of calluses from stem nodes and leaf tissues of Capsicum chinenseJacquin under different doses of auxins and cytokininsto determine the best conditions for in vitrocallogenesis. Callus induction and the area covered by callus cells in explants of cumari-do-para was induced using 2,4-dichlorophenoxyacetic acid and 6-Benzylaminopurine in different concentrations. It was observed that the percentage of callus induction and the percentage of callus-covered cell area in both shoot and leaf explants were higher when 2.0 mg L-1and 1.0 mg L-1of 2,4-dichlorophenoxyacetic acid. The highest callus formation and large callus area were obtained from stem nodes when grown in culture medium supplemented with the combination of 2 mg L-12,4-dichlorophenoxyacetic acid + 0 mg L-16-Benzylaminopurine.